KR100753186B1 - A anti-aging cosmetic composition containing a herb extracts mixture - Google Patents

Abstract

An anti-aging cosmetic composition containing a mixture of herb extracts is provided to promote growth of skin cells, inhibit oxidation, improve skin wrinkles and elasticity by promoting collagen synthesis, moisturize the skin and increase an immune response in the skin without side effects. The anti-aging cosmetic composition contains 0.0001-30 wt.% of a mixture of herb extracts which consist of Pleuropterus multiflorus, Poria cocos, Achyranthes japonica, Angelica gigas Nakai, Lycium chinense, Cuscuta japonica Choisy and Panax schinseng and are prepared by extracting with water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, chloroform or 1,3-butyleneglycol. The anti-aging cosmetic composition is formulated as cosmetic lotion, essence, lotion, cream, pack, gel, ointment, patch or spray.

Description

A anti-aging cosmetic composition containing a herb extracts mixture

The present invention relates to a cosmetic composition for preventing skin aging containing a herbal extract mixture, and more particularly, a herbal extract mixture consisting of sesame, baekbokyeong, dew, donkey, wolfberry, earth and sand and ginseng extract (hereinafter referred to as "chilbo yeodan") It relates to a cosmetic composition for preventing skin aging containing a) as an active ingredient.

As the largest organ of the human body, which occupies about 16% of the total body volume, the skin is in direct contact with the external environment and is important to protect the body from many deadly harmful factors such as temperature, humidity, and ultraviolet radiation. Act as a shield. However, as they age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111: 31-37 (1996)). Intrinsic aging is a aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. Clinically, the elasticity is reduced, the skin texture is rough, deep wrinkles are formed, and pigments are deposited. Arch Dermatol., 130: 87-95 (1994). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a marked decrease in elasticity.

Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, when the skin is exposed to harmful free radicals or free radicals, lipid peroxide is produced by oxidative action in the body and causes modification of various proteins constituting the skin. Meanwhile, collagen and elastic elastin proteins, which make up most of the dermis (about 70-80% of the total dry weight of the skin), are the major proteins produced by fibroblasts and are involved in the skin's mechanical firmness, tissue adhesion, and elasticity in the dermal layer. (Journal of the american academy of dermatology, 21: 610-613 (1989)). Collagen decreases with age and with increasing exposure to ultraviolet light. Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen types I and III constitute the constituents of the cytoplasm of the dermis, and collagen type IV is the major component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermal and dermal layers of the skin to regulate the permeation and transport of materials between the two layers, to control the differentiation of adjacent skin cells and to maintain the firmness of the skin. Collagen and elastin, the major structural components of the skin, are either denatured or reduced in biosynthesis by natural or external stimuli, thereby accelerating skin aging.

In order to suppress the skin aging or to slow the progression, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids and tocopherol have been developed. However, such cosmetics or skin protectors have a problem in that the potency is low in the final formulation, the efficacy is slight, and the skin irritation is severe.

On the other hand, each herbal extract used in the present invention is a component already known in cosmetics. In case of sewage, moisturizing (Korean Patent Publication No. 2005-0110720), Anti-aging (Korean Patent Publication No. 2005-0073918, Korean Patent Publication No. 2005-0002581), Whitening (Korean Patent Publication No. 2003-0021811) There is an example used. In addition, Baekbongryeong was used for anti-aging (Korean Patent Publication No. 2003-0039389), atopy improvement (Korean Patent Publication No. 2004-0065126), and whitening (Korean Patent Publication No. 2003-0076829). In addition, in case of dew, it was used for hair loss prevention (Korean Patent Publication No. 2003-0079075) and whitening (Korean Patent Publication No. 2002-0044266). In addition, Angelica was used to prevent skin aging (Korean Patent Publication No. 2005-0073918). In addition, goji berries were used for skin aging prevention (Korean Patent Publication No. 2005-0080893), skin moisturizing (Korean Patent Publication No. 2001-0067911). In addition, earth and sand were used to prevent skin aging (Korean Patent Publication No. 2003-0061847). In addition, ginseng was used to prevent skin aging (Korean Patent Publication No. 1999-0086516).

However, cosmetics have not been mentioned since they contain all of the sewage, Baekbongryeong, hyssop, Angelica, Gugija, Tozasa and Ginseng used in the present invention.

The present inventors have diligently researched to develop a cosmetic composition for preventing skin aging, ie, skin protection using folk medicines and herbal herbal medicines, and as a result, a herbal extract mixture containing sewage, baekbokyeong, dew, donkey, wolfberry, earth and sand and ginseng extracts Promotes proliferation, antioxidant effects, promotes collagen synthesis, enhances skin immunity and improves skin moisturizing effect. Cosmetics containing the herbal extract mixture as an active ingredient include complex skin aging such as wrinkles, reduced elasticity and loss of moisturizing ability without skin irritation. The present invention has been completed by confirming that it is possible to effectively protect the skin by improving skin immunity by eliminating the phenomenon and having an excellent anti-aging effect.

Accordingly, it is an object of the present invention to provide compositions for skin cell proliferation and antioxidant.

Another object of the present invention to provide a composition for enhancing collagen synthesis.

Another object of the present invention to provide a composition for enhancing skin immunity.

Another object of the present invention to provide a skin moisturizing composition.

Another object of the present invention to provide a complex skin anti-aging cosmetic composition without skin irritation and side effects.

The present invention provides a cosmetic composition for preventing skin aging, comprising a herb extract mixture consisting of sewage, baekbokyeong, dew, donkey, wolfberry, earth and sand and ginseng extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present inventors conducted research on various substances in order to search for excellent anti-aging efficacy substances from natural natural products, which have been conventionally used as herbal medicines, and as a result, sewage, baekbokyeong, dew, donkey, wolfberry, earthenware and ginseng extract The herbal medicine extract mixture consisting of Chilbo Miryeodan confirmed that the skin cell proliferation promotion, antioxidant efficacy, collagen synthesis promotion, skin immunity enhancement and skin moisturizing effect is excellent and completed the present invention.

Chilbo Mirdan provided in the present invention is obtained by the mixing of sewage, Baekbokyeong, hyssop, Angelica, wolfberry, earth and sand and ginseng extract.

The sewage (Scientific name: Pleuropterus multiflorus ) is a perennial herb belonging to the Polygonaceae family and is known to come from China and is a long-growing vine medicinal plant. The height is 3 ~ 4m and the roots are extended to the side, often forming tubers. Heart-shaped leaves are about 3-6cm long and 2.5 ~ 4.5cm wide, with petioles and chin leaves, but without jagged edges. Flowers bloom in August-September in conical inflorescences. There are no petals and 5 calyxes. Fruits are ovate, glossy, 2.5mm long. In Korea, there is P. cilinervis on Jirisan, which is different from sewage because its leaves are large and the fruit is wrapped in flower cover with triangular ovate without wings. Tuber roots are used as tonics, tonics, tong economy, yangjeung, antitussives and used to treat colds, hemorrhage, nervous breakdowns, and arthritis.

The Baek Bok-Ryong (People: Poria cocos ) is a medicinal mushroom that has been used as a herbal material since ancient times as a mushroom of the fungus fungi Democracy. Medicinal is the fungus that this mushroom makes on pine roots in the ground, and fruiting bodies rarely form on the surface of the fungus when it is exposed to the surface. The bacterium is a potato or oval lump with a diameter of 10-30 cm, the surface is dark brown, and is covered with a hard wrinkled shell. Meat is similar to semi-dried cheese and is white in color. When the tissue is viewed under a microscope, many granules are mixed into the mycelium pieces and severely refract light rays. This is a variation of mycelium, which exhibits a dextrin reaction that changes color to dark reddish brown in potassium iodide solution. The fruiting body of Bokryeong spreads flat on the fungal nucleus, having only 2 to 20 mm of depth and 0.5 to 2 mm of pores arranged on the surface, showing no mushrooms, and almost white in color. Diuretics, soothing agents, tonics, used for the treatment of edema, mental anxiety, palpitations, dyspepsia. In recent years, there has been an announcement that anti-tumor action is given to pakiman, a polysaccharide as one of the ingredients, and the medicinal effect is expected.

The hyssop ( Achyranthes japonica ) is a root of perennial herb, belonging to the amaranth family, and is used for food and medicine. Its main ingredient contains saponin and large amounts of calcium. Efficacy is under the action of blood circulation (活血 行 下) to induce women's physiology to normal, and to treat lobster, postpartum abdominal pain. In addition, palliative pain (緩和 止痛) acts to treat the inflammation caused by arthritis, rheumatoid arthritis, and bruises of the knee disease, and has the action of fish (·) and diuresis. Back and legs are heavy and painful, sometimes used for muscle cramps. In addition, the blood vessels are diminished by the temporary blood pressure lowering effect, which is used when there is a headache or dizziness of hypertension.

Angelica Angelica (scientific name: Angelica gigas Nakai ) is the root of Angelica perennial, a perennial aromatic herb belonging to the family Apiaceae. Looking at the plant shape of the donkey 60-90㎝ high. Flowers are white and bloom at the end of main stems and branches in August-September. Fruits are long, long oval, with narrow wings at the edges. The medicinal form of Angelica gigas leaves stem and leaf residues from the coarse and short roots. The main root is about 3 ~ 7㎝ long, 2 ~ 5㎝ diameter and the branch root is 15 ~ 20㎝ long. The outer surface is light yellowish brown to blackish brown, and there are many vertical wrinkles in the root and branch roots, and there are also horizontal wrinkles in the main root. Angelica is a representative herbal medicine of blood remedies among herbal medicines to send blood and improve the clearing and blood flow. Therefore, it is greatly used for pain resulting from anemia or blood circulation disorder caused by blood. It also promotes peristalsis of the intestines and helps the body to absorb enough nutrients by releasing the gas smoothly. The components of Angelica mucin include essential oils and pyranucmarins of decursinol, decursin, nadakenetine, umbeliferon, beta-sitosterol and other water-soluble nonvolatile crystals.

The wolfberry (scientific name: Lycium chinense ) is a deciduous broad-leaved shrub that grows wild around the village. In the deep soil, the main stem leans obliquely and sags to the bottom and grows well. The branches have common thorns, the outer shell is gray, and the leaves are fresh and ovate or lanceolate. The flowers continue to bloom in summer, and the fruits ripen in red oval in autumn. The leaves are edible and the fruits are used for medicinal purposes. The name of each part is called Goji berries, leaves are Goji leaves, and the roots are called Jigolpi (地骨皮). Since ancient times, it is known as a mysterious medicine of the unbroken life, the fruit contains betaine, the skin is pisalin, and the leaves contain rutin. It contains a lot of vitamin A and contains a lot of minerals such as phosphorus, carbohydrates and protein. The leaves contain vitamin C, which strengthens the walls of blood vessels such as capillaries and prevents arteriosclerosis, and the fruit contains ingredients that improve blood circulation. Long-term use of wolfberry prevents the disease and strengthens the body, especially for people with high blood pressure. It can lower blood pressure and prevent strokes. In addition, there is a tonic action, antipyretic action is good for patients with fatty liver with liver oil due to eating a lot of diabetes, alcohol, meat, etc., accompanied by a weak back or inflammatory thirst of the chest. Especially for women, the skin becomes hard and no blemishes. This has been used as a cosmetic for roots, stems, leaves, and berries of goji berry. Eyes become dim, dizziness and tired people, the production of immune enhancing substances, hematopoietic and growth hormone is also effective in promoting the effect. There is an action to increase the phospholipids in the serum and liver and to promote the creation of hepatocytes, and to stimulate the central and peripheral parasympathetic nerve.

Cuscuta japonica Choisy is a perennial herb that grows on vines and grows in hills, fields and hills. The main stem is yellow, wire-shaped, hairless, seeds germinate, parasitic on other trees, absorbs nutrients, and grows tangled about 5m long. The leaves are like scales, 0.2cm long, triangular, and flowers are white in watery inflorescences. Small peduncles are short or short, and several are clustered together. The seeds are called tosa, and the seeds are rich in resin-like glycosides, amylases, and provitamin A. In oriental medicine, it was used as a gangjeong tonic, oil well, low back pain, and also as a sedative, analgesic and diarrhea. In the private sector, the face was washed with starch juice to remove papules. It has also been used as a sputum and blood drops.

Ginseng (Scientific name: Panax schinseng ) is a plant called perennial perennial herb, or the root of it, belonging to the genus Asteraceae. Also called ginseng . Originally a wild plant that grows in the forests of temperate mountain areas, it is grown as demand for medicinal plants. Plants grown sufficiently for many years are about 60 cm tall, with only one stem growing straight or obliquely each year, with 3-4 leaves at the end. The leaves have long petioles, and the leaves usually form palm-shaped double leaf divided into 5 small leaves, 3 of which are large (4.5-1.5cm long, 3-5.5cm butterfly) and 2 smaller. Small leaves are pointed or egg-shaped, with fine jagged teeth on the edges, with hairs scattered on the upper veins. In the summer, one slender stem grows at the tip of the stem, and at the end of this, 14-40 pale yellow-green small flowers bloom in the shape of umbel. The flower consists of 5 petals and 1 pistil. In autumn, small, wide-spread spherical crimson fruit is produced. Fruits are 5-9 ㎜ in diameter, the nucleus is a semicircular nucleus in it, and each contains one seed in the nucleus. Roots are yellowish white on the outside and grow a little over a year after germination, with lengths of 10 ~ 20㎝ and 2 ~ 3㎝ thick. In addition, the roots are similar in shape to carrots, but have strong branching properties, so that the roots of many fingers and many small roots are split from the roots, so the roots resemble those of the human body. It is called ginseng. The root of ginseng has various medicinal effects such as nourishment, tonic, kangsim, correction (건 精), health, and soothing. It has little toxicity and has been used as a herb and herbal medicine for long-term rule, long life. In recent years, many such effects have been recognized scientifically. In general, the ginseng's medicinal effects, which are recognized in Korean medicine, are widely used as medicines such as tonic, stiffness, health, correction, soothing, and nourishment. It is also used as an agent for stomach illness, indigestion, vomiting, chest pain, laxative diarrhea, and loss of appetite.

The various herbal components in the "chilbo yeodan" mixture provided in the present invention may be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous having 1 to 4 carbon atoms. Or a hydrous lower alcohol (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol with water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3 -Butylene glycol can be obtained as an extraction solvent.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also herbal extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the aforementioned extraction solvent, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

The cosmetic composition having an anti-aging effect according to the present invention is not particularly limited in its formulation, but may be prepared, for example, in a formulation such as lotion, essence, cream, pack, gel, lotion, ointment, patch or spray. have. The content of the herbal extract mixture in the formulation may be contained in 0.0001 to 30% by weight, preferably 0.001 to 20% by weight relative to the total weight of the composition. If the content of the mixture is less than 0.0001% by weight relative to the total weight, it is difficult to expect the desired effect in the present invention, if it is more than 30% by weight may cause a problem in formulation stability.

The cosmetic composition of the present invention has the use of skin anti-aging (skin protection). As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging. Also, in this specification, "anti-aging of skin" and "skin protection" are used in the same sense unless otherwise specified.

The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and skin elasticity, moisturizing and skin immunity by promoting the proliferation of skin cells, anti-oxidation effect, promotion of collagen synthesis, and the like. It is specified in the example. Thus, skin aging prevention (ie, skin protection), which is a use of the present invention, is construed to include wrinkle improvement, moisturizing improvement and skin elasticity improvement use.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Production Example  One: " Cloisonne Beauty Group "Preparation of herbal extract mixture

Firstly, after completely drying the sewage, Baekbokyeong, hyssop, Angelica, wolfberry, earthenware and ginseng, respectively, sewage 40g, Baekbokyeong 40g, dew 10g, donkey 10g, wolfberry 10g, earthenware 10g and ginseng 5g After mixing by weight, 10 to 20-fold volume of water was added as an extraction solvent. It was heated at 70 ° C. for about 1 hour. Subsequently, the mixture was cooled and then cooled to room temperature, and then aged at low temperature at 4 ° C. for 7 days, followed by filtration.

Experimental Example  1: skin cell proliferation effect

In order to examine the effect of Chilbo-Ryodan on skin cell proliferation, human normal fibroblasts (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate. Incubated in DMEM medium (Sigma) for 24 hours at 37 ℃. Subsequently, the herbal extract mixture was replaced with the serum-free DMEM medium containing the serum concentrations of 0.01%, 0.05%, 0.1%, 0.5% and 1% and the experimental group without the herbal extract mixture. One control group was further incubated for 24 hours. Then, 10 μl each of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added thereto, followed by incubation for 4 hours. The medium was removed, shaken for 20 minutes by addition of 100 μl of DMSO solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in Table 1 below.

Cell proliferation effect (%) = [(absorbance of experimental group-absorbance of control group) / absorbance of control group] x 100

Sample concentration (%) Absorbance (570nm) Cell proliferation effect (%) Control 0.631 - Cloisonne Beauty Group 0.01 0.765 21.2 0.05 0.854 35.3 0.10 0.981 55.5 0.50 1.064 68.6 1.00 1.245 97.3

As can be seen in Table 1, compared with the control group not treated with the herbal extract mixture, the experimental group treated with the herbal extract mixture shows a very high fibroblast proliferation effect.

Experimental Example  2: antioxidant effect

Free Radical Scavenging Activity Test was conducted to investigate the antioxidant effect of the "chilbo Miranda" herbal extract mixture obtained according to the preparation example of the present invention. The free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), and the stable free radical DPPH (1,1-diphenyl-2- picryhydrazyl (Sigma) reagent was used. An experimental group was prepared in 150 μl of 0.2mM DPPH solution (ethanol in the case of Blanc) at various concentrations, and 150 μl of these mixtures were added to each mixture, mixed for 30 minutes at room temperature, and then absorbed at 517 nm. As a control for this, it was set as the case using purified water. After measuring the absorbance for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below and the results are shown in Table 2 below.

Free radical scavenging effect (%) = 100-[(absorbance of experimental group-absorbance of blank) / absorbance of control] x 100

Cloisonne Mir concentration (%) Free radical scavenging effect (%) 0.01 20.2 0.05 38.1 0.10 57.9 0.50 79.2 1.00 93.7

As can be seen from the results shown in Table 2, the herbal extract mixture of the present invention was confirmed that the free radical scavenging ability is very excellent.

Experimental Example  3: collagen synthesis promoting effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the experimental group prepared with the "Chilbo Miranda" herbal extract mixture was replaced with serum-free DMEM medium containing the final concentrations of 0.01, 0.05 and 0.10% and serum-free DMEM medium without the herbal extract mixture. The control was further incubated for 48 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a kit (Takara, Japan). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

Sample concentration (%) Collagen Synthesis (ng / 2 x 10 ⁴ ) Control 124 Cloisonne Beauty Group 0.005 159 0.01 187 0.05 223

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the herbal extract mixture of the present invention increases, and thus the herbal extract mixture of the present invention shows an excellent collagen synthesis enhancing effect. .

Experimental Example 4: Skin Immune Enhancement Efficacy Test

After shaving the abdomen in 8-10-week-old C57BL / 6 mice, ultraviolet B wavelength (UVB) of 2.4KJ / m ² using FS-20 sun lamp was used to control the hypersensitivity of the control group. Compared to 44.3% suppressed immunosuppressive phenomenon.

The herbal extract extract of the present invention was applied after UV irradiation, and then sensitized with 3% trinitrochlorobenzene in the UV irradiation area after 3 days. After 6 days, 1% trinitrogen chloride was applied to the ears of mice and edema status was measured. The results are summarized in Table 4 below.

Sample concentration (%) Skin immune enhancement effect (%) Control - Cloisonne Beauty Group 0.01 12.6 0.05 24.5 0.10 39.5

As can be seen in Table 4, it can be seen that the herbal extract mixture of the present invention increases the skin immune response concentration-dependently.

Examples and Comparative Examples

To the cosmetic composition having a composition ratio of Table 5, the "chilbomi sweet" herbal extract mixture was prepared in the embodiment and 2% by weight based on the total weight of the composition and a comparative example was not included in the herbal extract mixture.

Experimental Example  5: moisturizing effect

In order to evaluate the moisturizing power of each of the cosmetic preparations prepared in Examples and Comparative Examples, the moisture retention ability and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Experimental Example  5-1: moisture Capacity  Measure

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the lower half of 30 test subjects, before application, after 1 hour of application, and The moisture content of the skin 2 hours after application was measured, and normal skin without any treatment was used as a control. The measuring device used a moisture content measuring instrument (corneometer CM825, Conrage + Khazaka, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 6 below.

division Example Comparative example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 83 69 50 Skin electrical conductivity 2 hours after application 73 57 50

Experimental Example  5-2: Measurement of moisture evaporation amount

After applying tape stripping to the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 5-1, and then use a moisture evaporator (Tewameter TM300, Conrage + Khazaka). Co., Germany Federal Bureau), the water evaporation amount was measured 5 times at 1 minute intervals every 12 hours to obtain an average value. The results are shown in Table 7 below.

  division Example (%) Comparative example (%) Control group (%) Moisture evaporation after 0 hours 100.0 100.0 100.0 Moisture evaporation after 12 hours 81.3 89.5 93.0 Moisture evaporation after 24 hours 63.5 76.6 85.3 Moisture evaporation after 36 hours 48.0 65.2 79.5 Moisture evaporation after 48 hours 31.5 53.0 72.6

As shown in the results of Experimental Examples 5-1 and 5-2, the Example of the present invention has a higher electrical conductivity of the skin and a smaller amount of water loss than the Comparative Example. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example  6: skin elasticity effect

The skin elasticity enhancing effect of the cosmetic composition containing the "chilbo beauty sweet" herbal extract mixture of the present invention was measured.

20 healthy women (average age 37 years old) of 20 years or older (mean age 37 years) were divided into 2 groups under the conditions of temperature 24-26 ° C and humidity 75. After 12 weeks of application (morning and evening), skin elasticity was measured using a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, Germany). The test results are described as R8 (R8 (12 weeks) -R8 (0 weeks)) values of cutometer MPA580, where R8 values indicate the nature of the viscoelasticity, and the results are shown in Table 9 below.

division Skin elasticity effect Example 0.61 Comparative example 0.29

As shown in the results of Table 8, it was confirmed that the skin elasticity of the example containing the herbal extract mixture is more than doubled compared to the comparative example does not contain the herbal mixture extract.

Experimental Example  7: human skin Patch test

In the past, the skin irritation test of the cosmetic compositions of Examples and Comparative Examples was carried out on 20 women in their 20s and 30s who had no hypersensitivity reactions to skin irritation and had no skin disease or skin allergy symptoms. I checked it. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland) and then airtightly wrapped in the forearm of the subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. After 1 hour and 24 hours, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to check for erythema and edema.

Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG), and the results are shown in Table 9 below.

-Evaluation criteria and score of skin reaction-

     Symbol Score Criteria

      -0 no response

      ± 0.5 faint or light erythema

      + 1 clear but weak erythema, edema and papules

      ++ 2 distinct erythema, papules and vesicles

      +++ 3 Severe erythema and alveolar, scleroderma

       Mean score = [Score X No. of response X 100 X 1/2] / [3 (Maximum score) X total number of subjects (n)]

As can be seen in Table 9, it was confirmed that the embodiment containing the herbal extract mixture is a very safe cosmetic composition without skin irritation and side effects.

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition containing the "chilbo Miranda" herbal extract mixture of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation example  1: Softener (skin lotion)

As shown in the following table, the softening lotion was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Cloisonne Beauty Group 1.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation example  2. Nutritional Cosmetics ( Milk Croissant )

Nutrients were prepared according to a conventional method as shown in the table below.

Compounding ingredients Content (% by weight) Cloisonne Beauty Group 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation example  3. Nutrition Cream

Nutritious cream was prepared according to a conventional method as shown in the following table.

Compounding ingredients Content (% by weight) Cloisonne Beauty Group 10.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation example  4. Massage Cream

Massage cream was prepared according to a conventional method as shown in the table below.

Compounding ingredients Content (% by weight) Cloisonne Beauty Group 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation example  5. Pack

Packs were prepared according to conventional methods as shown in the table below.

Compounding ingredients Content (% by weight) Cloisonne Beauty Group 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

As described above, the present invention provides a cosmetic composition for preventing skin aging containing “chilbo yeodan”, which is a herbal extract mixture consisting of sewage, baekbokyeong, hyssop, donkey, wolfberry, earth and sand and ginseng extract as an active ingredient. The cosmetic composition of the present invention prevents skin aging by promoting skin cell proliferation, antioxidant efficacy, skin wrinkle improvement and skin elasticity, moisturizing and skin immunity by promoting collagen synthesis. Furthermore, the cosmetic composition of the present invention is a safe substance having no skin irritation, and may be used in the formulation of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

Claims (6)

A cosmetic composition for preventing skin aging, characterized in that it contains a herbal extract mixture consisting of sewage, Baekbokryeong, dew, donkey, wolfberry, earth and sand and ginseng as an active ingredient. The cosmetic composition for preventing skin aging according to claim 1, wherein the herbal extract mixture is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition. According to claim 1, wherein the herbal extract mixture is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol Skin aging preventing cosmetic composition, characterized in that the extraction.        The cosmetic composition for preventing skin aging according to claim 1, wherein the herbal extract mixture is characterized by promoting skin cell proliferation, promoting antioxidant, promoting collagen synthesis, or enhancing skin immunity. The cosmetic composition for preventing skin aging according to claim 1, wherein the cosmetic composition has a formulation of a lotion, an essence, a lotion, a cream, a pack, a gel, an ointment, a patch, or a spray. A cosmetic composition for enhancing skin moisturizing, comprising a herbal extract mixture consisting of sewage, baekbokyeong, dew, donkey, goji, earth and sand and ginseng as an active ingredient.