KR102057829B1 - A cosmetic composition comprising beta-glucan isolated from mycelium of shizophyllum commune fr. or a culture thereof as an active ingredient and a vacuum-filled ampule containing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising beta-glucan isolated from mycelium of Shizophyllum commune or a culture thereof as an active ingredient, and a vacuum-filled ampule comprising the same. The present invention does not include artificial preservatives and alcohols, thereby having no skin irritation when used as a cosmetic composition, and has excellent skin moisturizing, wrinkle reduction and skin whitening effect. In addition, the present invention can be used as a cosmetic, as the state does not change even during long time distribution, even though a preservative is not used, thereby improving the convenience at the time of single use.

Description

A cosmetic composition comprising beta-glucan isolated from a chia mushroom mycelium or a culture thereof as an active ingredient and a vacuum filling ampoule comprising the same. or a culture according as an active ingredient and a vacuum-filled ampule containing the same}

The present invention relates to a cosmetic composition comprising a beta glucan isolated from the mushroom mushroom mycelium or its culture as an active ingredient and a vacuum filling ampoule comprising the same. More specifically, when used as a cosmetic composition, it contains a pure beta glucan does not contain alcohols as an active ingredient, and although it does not contain a preservative, relates to a vacuum filling ampoule that does not change the state even for a long time distribution.

The skin is divided into three layers of epidermis, dermis, and subcutaneous fat tissue in order from the outside, and is a very important tissue that not only protects the human body while directly contacting the external environment but also has biochemical and physical functions.

The skin is covered with sebum, sweat, dust, and dead skin cells.In summer, when the outside temperature is high, or during puberty, when hormones are secreted, excess sebum can cause acne or seborrheic dermatitis. There is a possibility of being damaged by exposure to ultraviolet rays due to contamination and destruction of the ozone layer.

Therefore, the selection of functional products related to cosmetics or cosmetics used directly on the skin has emerged as an important issue in terms of protecting the skin with concern about side effects.

These cosmetics should be free of side effects, human-friendly, and safe to use on specific constitutions, atopic skin or sensitive skin.

Therefore, natural plant materials are not only excellent in safety, but also have various ingredients beneficial to the human body, and thus are preferred as ingredients that provide anti-inflammatory, moisturizing, whitening, and anti-aging effects.

Existing cosmetic compositions can be easily made microbial contamination during the manufacturing process, distribution process, or consumer's use, without the addition of chemical preservatives (designated ingredients), which may cause skin safety problems.

Labeled ingredients are those ingredients that must be labeled by the Korea Food and Drug Administration and have been legally designated for use if they are used in consideration of hazards to humans.

As the designated ingredients, tar colour, antiseptic, fungicide, antihistamine, sunscreen, gold leaf, and hair products include phosphate as a whole phosphate item.

Among these, a preservative is a drug which prevents the decay of a substance, and it is an antiseptic which prevents the flora and fauna organic substance from decaying by the action of a microorganism, and the agent added in order to preserve | preserve the substance is a preservative. In order to maintain the quality of food, medicine, and cosmetics for a long time, preservatives that prevent decay by microorganisms are essential.

In particular, cosmetics have a long shelf life, and in the process of use and storage thereof, there is a high possibility of contact with microorganisms, various carbon sources and nitrogen sources are combined, and the amount of moisture essential for microbial growth is higher than that of food. There are more and more needs and problems with preservative treatment.

If an appropriate preservative system is not established in cosmetics, microorganisms multiply during manufacturing and consumer use, causing metabolism of microorganisms, causing odors and odors, or discoloration and pH change of products. In addition, it causes harmful effects on the human body, such as causing irritation to the human body due to metabolites and microorganisms generated during the microbial metabolism process. Especially pathogenic microorganisms cause disease.

Thus, in cosmetics, preservatives are not simply additives added to prevent spoilage of the product, but are irreversibly or from the time the cosmetics are manufactured at the factory until the end of their use by the consumer. By suppressing the growth of microorganisms that are accidentally contained or contaminated in the product, it can be referred to collectively as additives added for the purpose of preventing the deterioration, odor, deterioration of cosmetics caused by the growth of microorganisms.

However, these chemical preservatives inhibit the growth of microorganisms to impart safety and stability to cosmetics, but may also cause irritation to the user's skin. Representative examples include formalin free preservatives. Formalin free preservatives, including diazolidinylurea and imidazolidinylurea, have recently been reviewed for safety around the world, and their use is on the decline. Its use is limited to cleaning products and some wash off products. It is also a preservative that is banned or strictly controlled in some countries, including Japan.

In addition, even parabens preservatives, which are the safest as conventional chemical preservatives and are widely used in cosmetics and medicines, are allergic to skin (Andrea Counti et al., Contact Dermatitis, 1997, 37, 35-36) and potential as environmental hormones (Edwin et al. , Toxicology and Applied Pharmacology, 1998, 153, 12-19) and resistant bacteria. In addition, the use of accepted standards has been distrusted, and new problems such as acute, chronic toxicity, and mutagenesis due to persistent body accumulation are emerging (Xinhua, Food Science and Industry, 1990, 23 (4) 68-72).

In the case of the aforementioned plant natural material, it is not only excellent in safety but also has various components beneficial to the human body, and when used as a cosmetic composition, it may have various beneficial effects.

However, the natural plant material is prone to decay when distributed for a long time due to its properties, and when used as a cosmetic, there may be a problem that the quality is reduced by contact with oxygen in the air.

In order to improve such a problem, it is necessary to develop a product that can be stored for a long time without using a preservative, as well as providing a natural material with excellent skin moisturizing, wrinkle improvement and skin whitening effect.

KR 10-0295623 B1

It is an object of the present invention to provide a cosmetic composition comprising a beta glucan isolated from a mushroom mycelium or a culture thereof as an active ingredient and a vacuum filling ampoule comprising the same.

Another object of the present invention does not include artificial preservatives and alcohols, beta glucan isolated from the mushroom mushroom mycelium or its culture having no skin irritation, excellent skin moisturizing, wrinkle improvement and skin whitening effect when used as a cosmetic composition It is to provide a cosmetic composition and a vacuum filling ampoule comprising the same as an active ingredient.

Another object of the present invention, even without using a preservative, it can be used as a cosmetic because the state does not change even during long-term distribution, beta isolated from the mushroom mushroom mycelium or its culture that has improved the convenience of single use It is to provide a cosmetic composition comprising a glucan as an active ingredient and a vacuum filling ampoule comprising the same.

In order to achieve the above object, the cosmetic composition comprising a beta glucan according to an embodiment of the present invention as an active ingredient beta glucan (β-glucan) isolated from the mushroom (Schizophyllum commune) or its culture as an active ingredient It is excellent in skin moisturizing, wrinkle improvement and skin whitening effect.

The betaglucan is β- (1,6) -branched (1,3) -glycan.

The cosmetic composition includes beta glucan, 1,2-hexanediol, glycerin and purified water.

The vacuum filling ampoule according to another embodiment of the present invention is a cosmetic container in which a lid is sealed to the main body, and the inside of the main body includes the cosmetic composition according to the above, and the upper part of the main body coupled to the lid is a lid. When it is removed and the external force is applied to the main body portion, an outlet portion for discharging the cosmetic composition is formed, the main body portion is filled in a nitrogen state when filling the cosmetic composition, and the lid is sealed and the inside is filled with nitrogen and the cosmetic composition will be.

The cosmetic composition does not contain a preservative and does not come in contact with oxygen, so that the state does not change even during long-term distribution.

Hereinafter, the present invention will be described in more detail.

Cosmetic composition comprising a beta glucan as an active ingredient according to an embodiment of the present invention comprises a beta glucan (β-glucan) isolated from Schizophyllum commune or its culture as an active ingredient, moisturizing skin, wrinkles Improves and whitens the skin.

The beta glucan is more specifically characterized as β- (1,6) -branched (1,3) -glycan.

In order to obtain beta glucan, it must be extracted or separated from a natural product containing beta glucan, such as a skirt mushroom.

At this time, in the case of using an extraction step, it is generally used to extract and use an organic solvent as an extraction solvent.

In the case of extraction with an organic solvent, the beneficial components included in natural products are extracted, purified and fractionated to obtain, there is an advantage that can be produced in large quantities.

However, when the beta glucan extracted using the organic solvent is used as a cosmetic composition, as the organic solvent, which is an extraction solvent, is mixed together in the cosmetic composition, a problem may occur in which the organic solvent is delivered to the skin.

In other words, if the organic solvent is used in the process of separating the active ingredient from the natural product, the active ingredient can be separated within a short time, and it will be said to be very effective for mass production, but the natural extract extracted with the organic solvent is used as a cosmetic composition. In this case, not only the active ingredient of the natural extract is delivered to the skin, but an organic solvent is delivered together to cause skin irritation.

In general, ethanol, which is a representative organic solvent used in cosmetic compositions, has a high volatility, and thus, when used as one component of a cosmetic composition, can provide a refreshing feeling and is used as a cosmetic composition.

In addition, one can exhibit a disinfecting effect due to ethanol finely, there is a problem that can cause irritation to the skin.

In addition, in the case of ethanol, when used on the skin serves as a percutaneous absorption enhancer, it can promote the absorption of other harmful components contained in cosmetics. That is, when parabens are used as a preservative in cosmetic components, there may be a problem that parabens components can be easily absorbed into the skin by the action of ethanol.

In addition, there is a problem that skin inflammation or irritation occurs in people with aldehyde dehydrogenase deficiency.

In the case of alcoholic solvents that are easily used as organic solvents, it may be desirable to limit the use as a cosmetic composition, as it may cause irritation to the skin or act as a transdermal absorption accelerator as in the aforementioned ethanol.

The use of such a conventional chemical composition as a cosmetic composition is problematic, and the use of natural substances as cosmetic compositions, but when using an organic solvent to extract the active ingredients of such natural substances, the effect of using natural substances may be halved. There is a problem that can be.

Thus, in the present invention, in order to prevent such a problem, the chichi mushroom (Schizophyllum commune) or beta-glucan (β-glucan) isolated from the culture thereof is included as an active ingredient.

More specifically, to separate beta glucan from the culture prepared by culturing the skirt mushroom to use it as a cosmetic composition.

The beta glucan is a polysaccharide in which glucose is polymerized around β-1,3 chemical bonds, and is a beta glucan derived from microorganisms produced by separating from cell walls or microsaccharides of microorganisms such as mushrooms and yeasts. Glucan or β-1,3-1,6-glucan) and vegetable beta glucan (β-1,3-1,4-glucan) extracted from dietary fiber of cereals such as barley and oats. They can exhibit a variety of physiological activities according to more specific glucose binding structure, and are also used in a variety of high value-added biological materials such as cosmetic additives, health supplements, food additives, concrete admixtures, feed additives.

Β-Glucan, in the form of β- (1,6) -branched (1,3) -glucan, known to be present in Schizophyllan of the Schizophyllum commune, enhances immunity At the same time, natural immunomodulators that are not tolerated and their biological activity against anticancer and antioxidant are reported.

The beta glucan is known as BRM (biological response modifiers) that act on the body's immune system to enhance the body's immunity, and in particular, the beta glucan activates the function of macrophage in the immune system. It has been reported to enhance the overall function of the immune system by secreting cytokines such as interferon or interleukin, which are proliferation factors of other lymphoid or leukocytes.

Separating beta glucan from the culture of the skirt mushroom is specifically as follows.

The mycelium of the skirt mushroom was used mycelium obtained by germinating spores obtained from the fruiting body of the skirt mushroom from the wild. Yeast-potato starch-glucose agar medium (3g yeast extract, 10g potato starch 10g glucose, 15g agar, 1 L distilled water) and obtained by incubating at 24 ℃ temperature for 7 days. On the other hand, the mycelia were incubated in a test tube containing yeast-potato starch-glucose agar medium, stored at 4 ° C, and subcultured every month.

The mycelia grown on the slope medium are aseptically collected, and then inoculated in a liquid medium at 5% (v / v). As a liquid medium, glucose 5%, yeast extract 1%, malt extract 0.4%, skim milk 0.4%, potassium monophosphate 0.3%, iron sulfate 0.04% pH was adjusted to 5.5 in terms of mycelial growth and beta-glucan productivity Good. Liquid culture of the mycelium was incubated for 7 days under conditions of 28 ° C., agitation speed of 250 rpm, and aeration of 1 vmv in a fermentor. Skirt mushroom-derived beta glucan was recovered through the alcohol purification.

By separating the beta glucan from the skirting mushroom through the manufacturing method as described above, it can be produced directly, or can be used as a cosmetic composition by being supplied from Kyuzen Biotech.

The beta glucan is isolated from the culture of the mushroom mushroom mycelium without using an extraction solvent, the beta glucan with high purity separated skin is very excellent in moisturizing, wrinkle improvement and skin whitening effect.

However, compared to the case of using only beta glucan alone, when a small amount of the natural extract is added due to the synergistic effect of the mixed use with beta glucan can further increase the skin moisturizing, wrinkle improvement and skin whitening effect.

More preferably, the natural extract may further include Angelica gigas Nakai, Cicada extract (Populus alba glandulosa) and Shrimp extract (Crotalaria sessiliflora L.).

Angelica gigas Nakai (Angelica gigas Nakai) is a perennial herbaceous plant belonging to the genus ie, mountain-type family produced in Korea, mainly cultivated for medicinal purposes in Korea, Japan, China, etc. (Angelica acutiloba Kitagaw) and Chinese-made Angelica sinensis Diels, which are known to differ in composition and pharmacological effect. Since ancient times, Angelica has been used as a herb for young sprouts, and its roots have been used as a medicine for various diseases such as pain relief, anticancer, renal toxicity reduction, liver function improvement, treatment of diabetic hypertension, and blood circulation improvement.

The thorn tree (Populus alba glandulosa) is a species made by artificial breeding of P. alba and P. glandulosa 30 years ago. The heartwood is dark brown, and the sapwood is pale yellow. Due to the poor quality and mechanical properties of wood, it was not widely used in the wood industry. The bark of all Populus species, including these hawthorn trees, has a structure similar to aspirin, which is characterized by salicylic derivatives of salicylic acid and salicylic alcohol, which are widely used as anti-inflammatory, analgesic and antipyretic agents. Progressed.

The Sprouts (Crotalaria sessiliflora L.) is a year-old plant growing in the grass, 20-70 cm high, with the long hairs except for the leaf surface. Leaves are broad and broad, linear or lanceolate, pointed or dull. Flowers are blue purple and run like spikes on the ends of main stems and branches in July to September. Fruits are small, with brown hairs dense, oval, flat, and divided into two.

When cultivation of the mycelium mushroom mycelium and beta glucan isolated from it to be used as a complex extract by mixing the Angelica extract, thorny bark extract and bowel extract, moisturizing the skin, compared to using only beta glucan alone Wrinkle improvement and skin whitening effect are further enhanced.

The Angelica Angelica extract, Sashimi extract and Sanamul extract are subjected to the extraction process using purified water, without using an organic solvent as the extraction solvent. After drying and grinding the Angelica Angelica, Sashimi and Shrimp, the extract may be prepared by leaching using purified water 2 to 50 times based on the weight of the sample.

More specifically, 2 to 50 times purified water based on the weight of the sample is heated to 90 to 100 ℃, put the sample in the heated purified water, can be left for 1 to 72 hours for leaching, more specifically Prepared as extract by leaving for 24 to 48 hours. After extraction, the extract can be obtained in powder form by the method of concentration or lyophilization.

As mentioned above, although the manufacturing process of the extract takes some time, compared to the case of using an organic solvent such as ethanol, the organic solvent is not used to extract the organic solvent for use as a cosmetic composition, To prepare an extract, when used as a cosmetic composition, to prevent the occurrence of skin irritation by the organic solvent.

The cosmetic composition includes beta glucan, 1,2-hexanediol, glycerin and purified water, and may further include the natural extract.

Preferably, with respect to 100 parts by weight of purified water, 1 to 5 parts by weight of beta glucan, 0.5 to 1.5 parts by weight of extract of Angelica gigas, 0.5 to 1.5 parts by weight of hawthorn extract, 0.5 to 1.5 parts by weight of wild sprout extract, 1,2-hexane 5 to 10 parts by weight of diol and 20 to 30 parts by weight of glycerin.

When used within the above range, the skin moisturizing, wrinkle improvement and skin whitening effect can be maximized. If the value is less than or above the above range, the synergy between components is insufficient or economically ineffective. have.

The cosmetic composition of the present invention may be prepared by mixing a dermatologically acceptable carrier in addition to the beta glucan of the present invention.

The dermatologically acceptable carrier may include purified water, oils, waxes, fatty acids, fatty alcohols, fatty acid esters, surfactants, hygroscopic agents, thickeners, antioxidants, viscosity stabilizers, chelating agents, buffers, preservatives, lower alcohols, and the like. However, the present invention is not limited thereto, and the types and concentrations thereof are various and include a part that can be changed by those skilled in the art within the scope of the present invention.

However, in the present invention, in order to limit the use of chemical components such as alcohols, other components except 1,2-hexanediol and glycerin described above are not used, but the use of the above-exemplified components is not necessarily impossible. Ingredients that can be added to the cosmetic composition by those skilled in the art can be used without limitation.

In addition, functional substances such as whitening agents, moisturizers, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, dyes may be included, if necessary, which are amounts commonly used in the cosmetic field. It may be included in the cosmetic composition according to the present invention.

Cosmetics prepared from the cosmetic composition of the present invention may be prepared in the form of general emulsion formulations and solubilized formulations. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics.

In addition to the beta glucan of the present invention, the cosmetics may be prepared in the form of adjuvants that can be used topically or systemically as commonly used in the art by including a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, face washes, soaps, treatments or cosmetics. Etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing Formulations such as foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders or eye shadows.

In another embodiment of the present invention, it is possible to use the ampoule by adding the cosmetic composition.

Recently, many ampoules in cosmetic formulations have been released and sold. Ampoule is a term for a small glass container that seals a batch of injectables. Ampoule-type cosmetics is applied to the cosmetic field by hygienic packaging of highly concentrated ingredients that are easily deteriorated by external contact.

Ampoule-type cosmetics are characterized by high concentration of the main nutritional ingredients, to be used while dropping a small amount on the skin.

Due to the nature of the ampoule type cosmetics, the interior of the stopper is composed of a dropper form, or a number of products that can be used in combination with the dropper on the stopper has been released.

That is, when considering the characteristics of the muffle-type cosmetics to be used by dropping a small amount of skin, the inside of the stopper is configured in the form of a dropper, to remove some of the cosmetic composition in the cosmetic container, to fall to the skin, or to combine the stopper with the dropper, The cosmetic composition in the container may be made to fall outward through the dropper of the stopper.

In the case of commercially available ampoule cosmetics, it is provided in the form of direct contact with air, which is very likely to cause oxidation by oxygen, which means that the product that is oxidized is deformed and cannot be used and distributed for a long time. will be.

That is, the cosmetic composition used in the ampoule-type cosmetics has a problem of being easily oxidized with oxygen in the air because it uses a highly concentrated cosmetic composition as described above, since it contains a large amount of active ingredients.

In order to prevent such a problem and to be able to use for a long time, the conventional ampoule type cosmetics essentially contain a preservative.

That is, ampoule-type cosmetics are exposed to air by use of product characteristics and shapes, and due to this use state, there is a high possibility of being oxidized with oxygen in the air. In order to prevent such a problem, a small amount of a preservative is included to prevent oxidation.

However, even if a very small amount of preservative is added, the use of ampoule-type cosmetics to deliver a highly concentrated cosmetic composition to the skin, to deliver a beneficial ingredient, but when containing a preservative for a long time use, such a preservative component Since it is delivered to the skin, skin irritation occurs or skin trouble occurs.

In fact, the muffle cosmetics are used to deliver highly concentrated cosmetic compositions to the skin. When the preservatives are not used to provide no harmful ingredients to the skin, the skin improvement effect is excellent, but there is a problem that cannot be used for a long time. On the other hand, when using preservatives to enable long-term use, there is a problem of providing a preservative to the skin, causing skin irritation or skin troubles.

In order to improve such a problem, the present invention is a cosmetic container sealingly coupled to the body portion, the inside of the body portion includes the cosmetic composition according to claim 1, the top of the body portion coupled to the lid portion is removed the lid portion When the external force is applied to the main body portion, an outlet portion for discharging the cosmetic composition is formed, and when the cosmetic composition is filled, the main body portion is filled in a nitrogen state, and the lid is sealed, and the inside is filled with nitrogen and the cosmetic composition. To provide a filling ampoule cosmetics.

In the case of the vacuum filling ampoule, it is manufactured using a biodegradable polymer material, and is composed of a main body portion and a lid portion. The lid is configured in the form of a seal coupled to the top of the main body portion, the lid portion is characterized in that the outlet portion is formed so that the cosmetic composition contained in the main body portion can be discharged.

The main body is convex, and is pressed by an external force, and the cosmetic composition contained in the main body is brought out through the outlet by an external pressing force.

The outlet portion is configured in a cylindrical shape having a diameter of 0.5 to 1mm, even if the cosmetic composition of the main body portion is moved to the outlet portion by an external force, a large amount of the cosmetic composition is discharged to the outside one by one without being discharged.

 In addition, when filling the cosmetic composition into the main body portion is injected into the interior using a syringe-type injection device, the filling process is carried out under a nitrogen environment.

When the cosmetic composition is filled into the inside of the main body portion under a nitrogen environment, and the outlet portion and the lid portion of the main body portion are sealed, the inside of the main body portion is filled with only the cosmetic composition and nitrogen.

As described above, when the interior is sealed, the cosmetic composition is blocked from contact with oxygen so that the state change due to oxidation does not occur.

That is, the cosmetic composition is not in contact with oxygen or external harmful substances are not directly added, it is possible to use without changing the state for up to three years.

In addition, by limiting the amount of the cosmetic composition included in the main body portion to a single use amount, the ampoule cosmetic of the present invention can be used in a form that is discarded after a single use.

That is, as it is provided as a container that can be used once instead of being provided as a container for multiple use, it prevents the possibility of air contact and delivery of harmful substances by the use of ampoule cosmetics, and can be distributed and used for a long time. It is possible to provide.

More specifically, the use method can be easily removed by twisting the lid portion by an external force. Thereafter, the outlet part is positioned at the site where the cosmetic composition is to be applied, and when the main body part is pressed, the cosmetic composition is dropped by one to two drops through the outlet part.

Considering how to use the ampoule-type cosmetics used to deliver a small amount to the skin, a large amount can be configured to fall by one to two drops than delivered at a time can further improve the convenience of use.

The present invention relates to a cosmetic composition comprising a beta glucan isolated from the mushroom mycelium or a culture thereof as an active ingredient, and a vacuum filling ampoule comprising the same, and does not include artificial preservatives and alcohols, the skin when used as a cosmetic composition No irritation, excellent skin moisturizing, wrinkle improvement and skin whitening effect.

In addition, despite not using a preservative, the state does not change even during long-term distribution can be used as a cosmetic, it is to increase the convenience at the time of use.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention.

Preparation Example: Production of Cosmetic Composition

1. Preparation of Natural Extracts

The hawthorn leaves, stems and roots were washed, dried and crushed. The milled mixture was mixed with water and kept at 98-100 ° C. for 2 hours, cooled and filtered through Whatman filter paper to obtain the filtrate.

Angelica gigas and Shrimp were also prepared as hydrothermal extracts using the same method as the cultivars.

2. Preparation of Cosmetic Composition

Beta glucan isolated from the Schizophyll mycelium or its culture was used as supplied from Qyuzen Biotech Co., Ltd.

The beta glucan (BG), glycerin (GS), 1,2-hexanediol (HD), Angelica extract (AE), hawthorn extract (PE), bowel extract (CE) and purified water (WA) to the following table Mixing as described in 1 to prepare a cosmetic composition.

CM1 CM2 CM3 CM4 CM5 CM6 BG 5 0.5 One 3 5 10 GS 30 30 30 30 30 30 HD 10 10 10 10 10 10 AE - 0.1 0.5 One 1.5 2 PE - 0.1 0.5 One 1.5 2 CE - 0.1 0.5 One 1.5 2 WA 100 100 100 100 100 100

(Unit weight part)

3. Preparation of Vacuum Filled Ampoules

An outlet portion having a diameter of 1 mm was formed at the upper end portion, and the cosmetic composition was injected into the main body portion having an empty space therein in the form of an injection. During the injection, the injection process was carried out in a nitrogen environment, and the vacuum filling ampoule was prepared by sealingly coupling with the lid immediately after the injection was completed.

Experimental Example 1: Skin Moisturizing Effect

In order to evaluate the moisturizing power of the cosmetic composition, the moisture retention capacity and the water evaporation amount of the skin to which each cosmetic composition was applied were measured as follows.

Measurement of Moisture Retention Moisture retention capacity was measured using Corneometer (Courage + Khazaka, GmbH, Germany) to measure the electrical conductivity of the skin surface, and the measurement temperature was 22 ° C. and CM1 to 6 in a constant temperature and humidity room maintaining 45% relative humidity. This filled ampoule was applied to one cheek on 28 test subjects and the moisture content of the skin 2 hours after the application was measured. The relative and non-coated places where the cosmetic composition was applied were measured for relative comparison, and the results are described as average values as shown in Table 2 below.

CM1 CM2 CM3 CM4 CM5 CM6 ○ 60 62 70 74 78 61 Χ 50 48 50 53 54 51

(Wherein ○ is a ball coated with a cosmetic and Χ is a result of electric conductivity measurement on a ball not coated with a cosmetic).

According to the measurement results, the value of the high electrical conductivity means that the water content is excellent, it can be seen that the moisturizing effect is superior to the unused ball in the ball using the cosmetic composition of CM1 to CM6 by beta glucan have.

However, at 2 hours, it can be seen that a superior skin moisturizing effect appears in CM3 to CM5.

Experimental Example 2: Skin Wrinkle Improvement Effect

Among women over 40 years of age, those with wrinkles near the eyes were selected for questionnaire and physical examination. The eye area wrinkle was determined as the test site.

The ampoules filled with CM1 to CM6 were randomly assigned to the recruited experimenters, and then applied twice a day for 6 weeks to permeate the wrinkles around the eyes.

Four experimenters were assigned to each experimental group, and a total of 28 experiments were conducted. A replica was prepared for the facial wrinkle wrinkles, and a visometer (Visiometer, SkinVisiometer SV 600, Courage & Khazaka, Germany) ) Was used to analyze the image of skin wrinkle replica (Replica). Wrinkle analysis analyzed the change of skin wrinkle depth by grasping the depth of wrinkles by the light and shade of shadow generated by irradiating a certain angle of light on the miter plate.

The smaller the depth of wrinkles, the better the skin wrinkles.

CM1 CM2 CM3 CM4 CM5 CM6 Week 0 0.26 ± 0.12 0.27 ± 0.11 0.28 ± 0.14 0.26 ± 0.12 0.27 ± 0.11 0.26 ± 0.12 6 Weeks 0.25 ± 0.11 0.26 ± 0.14 0.25 ± 0.11 0.24 ± 0.10 0.27 ± 0.14 0.26 ± 0.11

(Unit mm, mean ± standard deviation)

According to the results of the experiment, the wrinkle improvement effect was partially confirmed in CM1, but the best wrinkle improvement effect was confirmed in CM3 to CM5.

Experimental Example 3: Skin Whitening Effect

Inhibition rate was determined by comparing the melanin production in the cells with B16F10 cell system. 1 × 10 ⁵ cells were incubated for 1 to 3 days in a 35 mm dish. To induce melanin production, the medium was treated with α-MSH or 3-isobutyl-1-methylanthine (IBMX) to promote melanin production.

The medium was removed, the cells were washed with PBS, 150 μl of 1N sodium hydroxide solution was added to the precipitated cells, and the cells were lysed and centrifuged at 80 ° C. in a thermostat. The supernatant was collected, the absorbance was measured at 490 nm, and the amount of melanin was calculated using a calibration curve using synthetic melanin.

Melanin production inhibition rate (%) = [(A-B) / A] * 100

A: Melanin amount in wells without sample

B: Melanin amount in wells to which sample was added

Concentration (mg / ml) (-) α-MSH (ug / ml) (+) α-MSH (ug / ml) Melanin
% Creation Melanin production inhibition rate (%) Control 0 268.1 637.6 100 0 Arbutin 0.1 370.4 58.1 41.9 CM1 0.1 399.2 60.5 39.5 CM2 1.0 399.2 62.6 37.4 CM3 1.0 387.4 48.1 51.9 CM4 1.0 249.4 39.1 60.9 CM5 1.0 240.6 37.7 62.3 CM6 1.0 431.7 67.7 32.3

As shown in Table 4, when compared with Arbutin, which is known to have an effect on skin whitening, it was confirmed that only beta glucan had excellent skin whitening effect at an equivalent level, and exhibited excellent skin whitening effect in CM3 to CM5. It was confirmed.

Experimental Example 4: Sensory Test

In addition to the skin wrinkle improvement effect test, an ampoule was applied to the wrinkles around the eyes, and a sensory test for applicability and absorption was conducted.

The evaluation score was requested to evaluate 1 to 5 points, the higher the score means that the excellent evaluation.

CM1 CM2 CM3 CM4 CM5 CM6 Application 2 3 4 4 5 One Absorbency 2 2 4 4 4 One

According to the sensory evaluation, it was confirmed that excellent evaluation in the application and absorbency in CM3 to CM5. According to the sensory evaluation, when providing a functional ampoule, it will be confirmed that it is possible to provide a high degree of muffle.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

Claims (5)

As a cosmetic composition comprising beta glucan (β-glucan) isolated from a schizophyllum commune or a culture thereof,
The cosmetic composition further comprises 1,2-hexanediol, glycerin, Angelica Angelica hydrothermal extract, Cinnabar hydrothermal extract, bowel hydrothermal extract and purified water,
The cosmetic composition is based on 100 parts by weight of purified water, 1 to 5 parts by weight of beta glucan, 0.5 to 1.5 parts by weight of hot Angelica extract, 0.5 to 1.5 parts by weight of Hibiscus hydrothermal extract, 0.5 to 1.5 parts by weight of hydrothermal extract, 1 5 to 10 parts by weight of 2-hexanediol and 20 to 30 parts by weight of glycerin,
Excellent skin moisturizing, wrinkle improvement and skin whitening effect
Cosmetic composition comprising beta glucan as an active ingredient. The method of claim 1,
The beta glucan is β- (1,6) -branched (1,3) -glycan
Cosmetic composition comprising beta glucan as an active ingredient. delete In the cosmetic container that the lid is sealed to the body portion,
The interior of the body portion comprises a cosmetic composition according to claim 1,
The upper portion of the main body portion coupled to the lid portion is formed when the lid portion is removed and the external force is applied to the main body, the outlet portion is discharged is formed,
When filling the cosmetic composition, the main body portion is filled in the nitrogen state and the lid portion is sealed and the inside is filled with nitrogen and the cosmetic composition
Vacuum filling ampoule. The method of claim 4, wherein
The cosmetic composition does not contain a preservative, does not come into contact with oxygen does not change the state even for a long time distribution
Vacuum filling ampoule.