KR102320873B1 - Composition for improving pet animals' skin and hair - Google Patents

Abstract

The present invention relates to a composition for improving the skin and fur of companion animals, containing a scutellaria extract, a cypress leaf extract, a rosemary extract, a cocoon extract, a ginseng extract, collagen hydrolyzate, and beta-glucan as active ingredients, and to a composition having excellent effects such as inflammation alleviation, the skin moisturizing, antibacterial activity, fur gloss enhancement, and prevention of fur static electricity.

Description

A composition for improving pet skin and fur {Composition for improving pet animals' skin and hair}

The present invention relates to a composition for improving the skin and fur of companion animals containing golden extract, cypress leaf extract, rosemary extract, cocoon extract, ginseng extract, collagen hydrolyzate, and beta-glucan as active ingredients, specifically It relates to a composition that relieves inflammation expressed on the skin of companion animals, exhibits a skin moisturizing effect, has antibacterial activity against disease-causing bacteria, and imparts gloss and elasticity to fur.

Beta-glucan is a substance present in the cell wall of mushrooms, yeast, grains, etc., and corresponds to a water-soluble polysaccharide dietary fiber. Specifically, polysaccharides polymerized around beta-1,3 chemical bonds are collectively called beta-glucan, and it is known that there are microorganism-derived beta-glucan and vegetable beta-glucan. According to the nutritional journal related to beta-glucan, when beta-glucan was fed to rats, it was confirmed that the number of lymphocytes, which are immune cells, increased, and it was confirmed that it was helpful in enhancing immunity. It corresponds to a substance widely used as an additive.

Since beta-glucan is a generic term for polysaccharides centered on beta-1,3 bonds, various structures including beta-1,4 bonds and beta-1,6 bonds can be formed even in beta-glucan, depending on the material to be extracted. form various structures. For example, beta-glucan extracted from oats and barley, including 1,4-beta-glucan, has a molecular structure that forms a single layer, exhibits high viscosity at a low concentration, and can be gelled. As beta-glucan may have various characteristics depending on the extraction source, extraction form, etc., the effects and uses are diverse. In particular, the anticancer effect is widely known, and in addition, it has skin improvement effects such as moisturizing and soothing effects, so it is used as a cosmetic material.
Gold is the root of gold ( Scutellaria baicalensis ) belonging to the genus Scutellaria of the Lamiaceae family, which grows widely in Europe and East Asia, as it is or refers to a product with the bark removed. Gold is widely known to have antipyretic, anticancer, and anti-inflammatory effects.
Cypress ( Chamaecyparis obtusa ) is an evergreen arboreous tree of the Japanese cypress family, also called cypress. In addition, in the case of cypress leaf extract, it is used as a part of human cosmetics to prevent skin damage.
Rosemary is a perennial plant belonging to the Lamiaceae family, and it is widely used as a raw material for human cosmetics because it gently soothes the skin.
Ginseng is a root of the Alaliaceae family and has been reported to have anti-fatigue, anti-aging, immune enhancement, and anti-cancer effects.
The silkworm is an insect belonging to the order Lepidoptera, and a cocoon is a type of house made by spitting out threads to surround the outer perimeter of the body when the silkworm pupates. In the past, it was raised by farmers to obtain silk thread, and it is known that it is rich in amino acids.
Collagen hydrolyzate refers to a product obtained by hydrolyzing collagen, which is the main protein of all connective tissues such as skin, blood vessels, bones, teeth, and muscles, using acids, alkalis, heat, and enzymes. It is known that when collagen is added to cosmetics, its moisturizing properties are improved, so it is widely used as an ingredient in basic cosmetics.
However, although each component is used as a component of cosmetics, it is known that it is used for human skin damage prevention, moisturizing, etc., and it is not known whether such an effect on the skin of companion animals is known.

It is known that the skin of most companion animals is covered with long hair, the dermis is thinner than that of humans, there are no pores, and the skin pH is about 8 to 9, which is slightly alkaline. In addition, since companion animals are densely covered with fur, they do not have good contact with the outside air, so even if a rash or inflammation occurs, it is difficult to check with the naked eye. On the other hand, the skin and fur of companion animals, such as dogs or cats, parasitize mites, Demodex and other various bacteria, which in many cases damage hair and cause hair loss. .

In the case of skin diseases, atopic dermatitis, vegetable allergy, contact allergy, flea allergy, Cushing syndrome, hypothyroidism, nail mitosis, alopecia, seborrhea, pyoderma, pyoderma phobiasis, trichomoniasis, parotidosis, yeast dermatosis, etc. Various diseases exist, and skin diseases are caused by various causes such as house dust, fleas, mites, pollen, drugs, plastics, wool rubber, shampoo, protein, and endocrine disorders. In addition, when a skin disease is induced, it is accompanied by itching, and there is a problem that the companion animal is more likely to be irritated by scratching itself. In addition, animal medicines can be used in relation to the treatment of skin diseases, but if not isolated from other animals, other animals may lick the drug and the treatment effect may not be easily seen. There are reports of side effects such as erythema, itchiness, hair loss, sagging, hyperexcitement, vomiting, and mucous membrane/eye irritation. However, skin diseases can be prevented by sanitary management of companion animals, grooming, provision of balanced food, and improvement of skin regeneration through hair or skin condition management. It can be easily treated through condition management, etc.

Accordingly, there is a demand for the development of a cosmetic composition for companion animals that can safely and effectively alleviate and improve skin diseases of companion animals, maintain the oil-water balance through moisturizing, and impart elasticity and gloss to the fur.
Accordingly, the present inventors have led to the present invention as a result of studying various naturally-derived ingredients and combinations thereof, including beta-glucan, which has a skin improvement effect.

Republic of Korea Patent Publication No. 10-2017-0114208 (2017.10.13.)

The present invention has been devised to solve the above problems, relieves inflammation expressed on the skin of companion animals, exhibits a skin moisturizing effect, has antibacterial activity against disease-causing bacteria, and provides luster and To provide a composition that gives elasticity

The present invention solves the above problems by providing a composition for improving the skin and fur of companion animals containing golden extract, cypress leaf extract, rosemary extract, cocoon extract, ginseng extract, collagen hydrolyzate, and beta-glucan as active ingredients Through various experiments, it has been confirmed that the present invention can be achieved.

In one aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract and ginseng extract may be an extract extracted through water, ethanol, or a mixed solution thereof.

In one aspect of the present invention, the cocoon extract may be an extract extracted through water, ethanol, or a mixed solution thereof.

In one aspect of the present invention, the collagen hydrolyzate may be a bovine collagen hydrolyzate.

In one aspect of the present invention, the beta-glucan may be extracted from Chima mushroom.

In a specific aspect of the present invention, the beta-glucan may be beta-1,6-branched-beta-1,3-glucan.

In one aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan are each 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, relative to the weight of the golden extract, 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, and 0.8 to 3.5 parts by weight.

In one aspect of the present invention, the composition may have anti-inflammatory or antibacterial activity.

In addition, in one aspect of the present invention, the composition can increase the oil and moisture content of skin and fur.

The present invention provides a cosmetic composition for companion animals comprising the composition for improving the skin and fur of the companion animal.

In one aspect of the present invention, the composition for improving the skin and fur of companion animals in the cosmetic composition may be 1.0 to 10.0 parts by weight based on 100 parts by weight of the cosmetic composition.

The present invention relates to a composition for improving the skin and fur of companion animals containing golden extract, cypress leaf extract, rosemary extract, cocoon extract, ginseng extract, collagen hydrolyzate, and beta-glucan as active ingredients, and has strong skin moisturizing power It has the advantage that it is possible to improve the skin and fur of companion animals through advantageous effects such as anti-inflammatory, skin irritation relief, deodorization, hair gloss enhancement, and fur static prevention.

Hereinafter, the present invention will be described in more detail through Examples and Experimental Examples. However, this is intended to help the understanding of the present invention and is not intended to limit the scope of the present invention.

The present invention relates to a composition for improving the skin and fur of companion animals containing golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan as active ingredients.

In the present invention, the "extract" includes an extract obtained by extracting substances such as gold, cypress leaf, rosemary, ginseng, and cocoon, and a solid dried product obtained by drying the extract by a method such as hot air drying or freeze drying. , a solvent dilution of the extract, a concentrate of the extract, a purified extract of the extract, or a combination thereof, and the like, and includes all types of extracts that can be formed using the extract itself and the extract.

In the present invention, the extract can be extracted from both natural forms of the respective plants, etc., as well as artificial forms such as mutated plants, mutated silkworms, hybrids, etc., and the form of the material for obtaining the extract is not limited. does not

In the present invention, the gold includes bikarin as a main active ingredient, and may further include other ingredients.

In the present invention, the rosemary includes flavonoids and terpenes, and may further include other components.

In the present invention, the ginseng contains saponin or ginsenoside as a main active ingredient, and may further include other ingredients.

In the present invention, the cocoon is a moth belonging to the subgenus Lepidoptera, and the cocoon may use various types of cocoons known in the art. The cocoon extract preferably contains 1-deoxynojirimycine. In one aspect of the present invention, the cocoon extract may be a golden cocoon extract.

In the present invention, the collagen hydrolyzate refers to a substance obtained by hydrolyzing known collagen, and includes all peptides in the hydrolyzed form such as monopeptide, dipeptide, and tripeptide. The method for hydrolysis may be carried out by a method known in the art, but is not limited thereto, but may be hydrolyzed through a hydrolase. Specifically, the collagen hydrolyzate may be a hydrolyzate of collagen obtained from an animal source.

In the present invention, beta-glucan includes all forms of glucose polysaccharides linked by beta-type glycosidic bonds. The beta-glucan may be included in the beta-glucan, even if it includes a 1,4-alpha bond, as long as the beta-type bond is the main bond.

In the present invention, the extraction method to obtain the extract is not particularly limited, and may be extracted according to a method commonly used in the art. For example, it includes hot water extraction method, filtration method, reflux extraction method, etc., which are exemplary and not limited thereto. In addition, the extraction method may be performed alone or may be performed through a method in which two or more types are sequentially or in combination.

In the present invention, the type of extraction solvent used to extract the gold, cypress leaf, rosemary, ginseng, cocoon, etc. is not particularly limited, and any solvent known in the art may be used. Examples include, but are not limited to, C1 to C4 lower alcohols such as water, methanol, ethanol, propyl alcohol, and butyl alcohol. In addition, the extraction solvent may be used alone or in the form of a mixed solution in which two or more types are mixed.

In one aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract and ginseng extract may be an extract extracted through water, ethanol, or a mixed solution thereof.

In addition, in one aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract and ginseng extract may be a dry extract obtained by extracting through water, ethanol or a mixed solution thereof, and then freeze-drying or spray-drying.

In a specific aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract and ginseng extract may be extracts extracted through ethanol.

In one aspect of the present invention, the cocoon extract may be an extract extracted through water, ethanol, or a mixed solution thereof.

During extraction, the extraction temperature may be 50 to 150° C., more specifically 90 to 120° C., but is not limited thereto. In addition, the extraction solvent may be added 15 to 25 times, more specifically 17 to 22 times the weight of each of gold, cypress leaf, rosemary, and ginseng used for extraction, but is not limited thereto. In addition, the extraction time may be 10 minutes to 10 hours, more specifically 2 hours to 5 hours, but is not limited thereto. In addition, the number of repetitions of the extraction may be 1 to 5 times, more specifically 2 to 3 times, but is not limited thereto.

In addition, in one embodiment of the present invention, the cocoon extract may be a dried extract obtained by extracting through water, ethanol, or a mixed solution thereof, then freeze-drying or spray-drying.

In a specific aspect of the present invention, the cocoon extract may be an extract extracted through water.

In the present invention, the cocoon extract may have a temperature of 90 to 120 ° C during extraction, and when the extraction temperature is less than 90 ° C, sterilization may not be performed properly, and when it exceeds 120 ° C, a preferred component of the silkworm extract transformation may occur.

In one aspect of the present invention, the collagen hydrolyzate may be a bovine collagen hydrolyzate.

In the present invention, the cow skin collagen refers to collagen contained in the hide or hide of livestock. In the present invention, the hydrolysis and the method of purifying the hydrolyzate may be performed by a method generally used in the art, and is not particularly limited.

In the present invention, the collagen hydrolyzate is pepsin, trypsin, α-chymotrypsin, papain, flavozyme, alcalase, neutrase (Neutrace), Bacillus subtilis ( Bacillus subtilis ) It may be hydrolyzed by a hydrolase, and specifically, it may be hydrolyzed by Bacillus subtilis ( Bacillus subtilis ).

In one aspect of the present invention, the beta-glucan may be extracted from Chima mushroom.

In a specific embodiment of the present invention, the beta-glucan may be beta-1,6-branched-beta-1,3-glucan extracted from Chima mushroom.

In the present invention, Schizophyllum mushroom includes all forms of Schizophyllum mycelium known to produce polysaccharides, and includes Schizophyllum commune Fr. However, this is illustrative and not limited thereto.

In the present invention, the beta-glucan may be extracted from the mycelium of Chima mushroom through an extraction solvent such as water or ethanol. The extraction method may be extracted according to a method commonly used in the art.

In one aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan are each 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, relative to the weight of the golden extract, 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, and 0.8 to 3.5 parts by weight.

In a specific aspect of the present invention, the golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan are each 0.8 to 1.2 parts by weight, 0.8 to 1.2 parts by weight relative to the weight of the golden extract. , 0.8 to 1.2 parts by weight, 0.8 to 1.2 parts by weight, 0.8 to 1.2 parts by weight, and 1 to 3 parts by weight.

When each extract is less than the range by weight, a desirable effective effect cannot be exhibited, and when it exceeds the range by weight, the effect of other active ingredients may not appear. In addition, by using the extract in an appropriate ratio, the composition of the present invention exhibits excellent effects such as anti-inflammatory effect, antibacterial effect, and oil/moisture enhancement.

The composition of the present invention has an effect that can improve skin and fur, and improvement of skin and fur can be achieved through moisturizing enhancement, antibacterial, anti-inflammatory, antioxidant, oil/water balance, oil enhancement, exfoliation, deodorization, etc. , and furthermore, it can exhibit effects such as enhancing fur luster and preventing static electricity.

In one aspect of the present invention, the composition is characterized in that it has anti-inflammatory or antibacterial activity.

In addition, in one aspect of the present invention, the composition is characterized in that it increases the oil and moisture content of the skin and fur.

In the present invention, the golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan promote collagen production, promote the production of skin cell growth factors, and provide high skin moisturizing power. have In addition, the composition is non-toxic and does not cause side effects even when applied to animals, so it can be used as a cosmetic composition for improving the skin and fur of companion animals.

The cosmetic composition of the present invention is an essence, solution, ointment, paste, cream, gel, foam, soft water, emulsion, soap, liquid detergent, suspension, emulsion, lotion, powder, surfactant-containing cleansing, oil, powder foundation, emulsion It can be prepared in all types of formulations, including liquid foundation, wax foundation, patch, and spray.

In the present invention, the cosmetic composition may further include other ingredients that are conventionally formulated in cosmetics, if necessary. For example, oil and fat ingredients, moisturizing agents, emollients, surfactants, organic pigments, inorganic pigments, organic powders, preservatives, fragrances, disinfectants, antioxidants, antioxidants, pH adjusters, alcohols, foaming agents, fillers, colorants, gelling agents, purified water and the like, which are illustrative and not limited thereto.

In the present invention, the cosmetic composition may further include an acceptable carrier as a cosmetic composition in the art. For example, one or more of oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, color, preservative, fragrance, etc. may be used, but the present invention is not limited thereto.

When the formulation of the cosmetic composition of the present invention is an ointment, paste, cream or gel, the carrier is animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, bentonite, silica, etc. may be used as a carrier, It is not limited thereto, and the carrier may be used alone or in combination of two or more.

When the cosmetic composition of the present invention is a soap, foam, liquid detergent or surfactant-containing cleansing agent, the carrier is an anionic surfactant, a nonionic surfactant, a fatty acid, an alkali metal salt of a fatty acid, an aliphatic alcohol, a vegetable oil, glycerol, glycerin , sugar, lanolin derivatives, etc. may be used as the carrier, but is not limited thereto, and the carrier may be used alone or in combination of two or more.

In the cosmetic composition of the present invention, the golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan may be included in an amount of 0.1 to 50% by weight based on the total weight of the cosmetic composition. More specifically, it may be included in an amount of 1.0 to 25% by weight based on the total weight of the cosmetic composition. More specifically, it may be included in an amount of 1.0 to 10% by weight based on the total weight of the cosmetic composition, and the weight ratio of each component is as described above. When it corresponds to the above weight part, as a cosmetic composition, the formulation such as the extract is in a stabilized form and can effectively exhibit the skin and fur improvement effect of companion animals.

Hereinafter, the present invention will be described in detail by way of Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

<Example 1> Preparation of each component of the composition

1. Preparation of herbal extracts

1) Preparation of Scutellaria Baicalensis Root Extract

10 g of dry gold, and 20 times the weight of dry gold, ethanol was added thereto, followed by extraction at 100° C. for 4 hours. Thereafter, it was freeze-dried at a temperature of -30 °C or less to obtain an extract in powder form.

2) Preparation of Chamaecyparis Obtusa Leaf Extract

10 g of dried cypress leaves and 20 times the weight of dried cypress leaves were added with ethanol, followed by extraction at 100° C. for 4 hours. Thereafter, it was freeze-dried at a temperature of -30 °C or less to obtain an extract in powder form.

3) Preparation of Rosemary Extract (Rosmarinus Officinalis Extract)

10 g of dried rosemary and 20 times the weight of dried rosemary were added with ethanol and extracted at 100° C. for 4 hours. Thereafter, it was freeze-dried at a temperature of -30 °C or less to obtain an extract in powder form.

4) Preparation of Panax Ginseng Root Extract

10 g of dry ginseng, and 20 times the weight of dry ginseng, ethanol was added, and extraction was performed at 100 ° C. for 4 hours. Thereafter, it was freeze-dried at a temperature of -30 °C or less to obtain an extract in powder form.

2. Preparation of Silkworm Cocoon Extract

After washing 500 g of golden cocoon, it was sterilized with high-temperature steam. Thereafter, purified water was added at 10 times the weight of silkworm larvae and extracted at 50° C. for 40 hours to obtain a cocoon extract.

3. Preparation of Collagen Hydrolyzate

The tissue was softened by adding purified water to bovine skin and 10 times the weight based on the weight of bovine skin, and applying heat at 50° C. for 6 hours. Then, the hydrolyzate Bacillus subtilis ( Bacillus subtilis ) was added and reacted for 1 day, followed by heat treatment at 100 ° C. for 10 minutes to obtain a collagen hydrolyzate.

4. Preparation of beta 1,6-branched-beta 1,3 glucan

The mycelium of Schizophyllum commune was added to the agar medium and cultured at 30 °C for 7 days. The cultured mycelium was aseptically collected and cultured for 7 days in a fermenter (27.5° C., 120 rpm) to obtain a culture solution. Then, purified water three times the weight of the culture medium was added to obtain beta-glucan derived from Chima mushroom.

<Example 2> Preparation of mixed composition

The golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate and beta-glucan prepared in <Example 1> were respectively 1.0 g, 1.0 g, 1.0 g, 1.0 g, 1.0 g, 1.0 g, and 2.5 g to prepare a mixed composition.

<Preparation Example> Preparation of a cosmetic composition

A cosmetic composition was prepared by adding the herbal medicine extract, cocoon extract, fish-derived collagen hydrolyzate, and beta 1,6-branched-beta 1,3 glucan prepared in Example above in the following configuration.

Preparation Example 1. Cream Formulation Cosmetic Composition composition Preparation Example 1 (%) golden extract 0.5 Cypress Leaf Extract 0.5 rosemary extract 0.5 Cocoon Extract 0.5 Collagen hydrolyzate 0.5 beta glucan 1.5 butylene glycol 10.0 propylene glycol 5.0 Arginine 1.0 squalane 5.0 polyethylene glycol 1.5 polysorbate 1.6 glycerin 5.0 paraffin 4.5 incense a very small amount Purified water to 100.0

Preparation Example 2. Essence-type cosmetic composition composition Preparation Example 2 (%) golden extract 0.5 Cypress Leaf Extract 0.5 rosemary extract 0.5 Cocoon Extract 0.5 Collagen hydrolyzate 0.5 beta glucan 1.5 propylene glycol 10.0 glycerin 10.0 ethanol 5.0 incense a very small amount Purified water to 100.0

Preparation Example 3. Ointment Formulation Cosmetic Composition composition Preparation Example 3 (%) golden extract 0.5 Cypress Leaf Extract 0.5 rosemary extract 0.5 Cocoon Extract 0.5 Collagen hydrolyzate 0.5 beta glucan 1.5 butylene glycol 2.0 wax 5.0 paraffin 17.0 polysorbate 0.5 glycerin 6.0 incense a very small amount Purified water to 100.0

Preparation Example 4. Shampoo type cosmetic composition composition Preparation 4 (%) golden extract 0.5 Cypress Leaf Extract 0.5 rosemary extract 0.5 Cocoon Extract 0.5 Collagen hydrolyzate 0.5 beta glucan 1.5 anionic surfactant
(Sodium dodecylbenzenesulfonate) 25.0 coco betaine 10.0 oil 1.0 glycerin 4.0 incense a very small amount citric acid a very small amount Purified water to 100.0

<Experimental Example 1> Check the anti-inflammatory effect of the composition

Dermatitis was induced by injecting 0.1 ml of histamine phosphate at a concentration of 0.01 mg/ml into the skin of three experimental dogs (2-3 year old female beagle). After confirming that dermatitis was induced, the mixture composition prepared in Example 2 was sprayed on the dermatitis-inducing site of the test dog three times at intervals of 6 hours a day for 3 days. As a comparative example, no action was taken on three experimental dogs (2-3 year old female beagle) that had dermatitis induced by the same method as above. In order to make the other conditions the same, the amount of feed eaten and the amount of water supplied were the same. Thereafter, the size of inflammation induced in the skin was measured on the fourth day after spraying the composition, and the measurement results are shown in Table 5.

experimental dog Initial size of inflammation (mm) Inflammation size (mm) composition spray group lab dog 1 8.0 2 lab dog 2 7.9 1.8 lab dog 3 8.1 2.4 composition spray group lab dog 1 8.2 10.1 lab dog 2 8.0 9.8 lab dog 3 7.9 11.1

As a result of the measurement, as shown in Table 5, in the case of the test dogs sprayed with the composition of the present invention, the size of inflammation was reduced, confirming the anti-inflammatory effect of the composition of the present invention.

<Experimental Example 2> Confirmation of antibacterial effect of the composition

As bacteria used to confirm the antibacterial effect, Staphylococcus aureus ATCC 6538P as Gram-positive bacteria, Escherichia coli ATCC 8739, and Pseudomonas aeruginosa ATCC 9027 as Gram-negative bacteria were used.

After inoculating a vial with a mixed suspension of three types of bacteria (S. aureus, Ecoli, P. aeruginosa), after storage at 25 ° C, samples are collected under aseptic manipulation at intervals of 1 day, appropriately diluted with a diluent, and stored at 37 ° C. After final incubation for 48 hours in a thermo-hygrostat, the diameter (mm) of the clear zone was measured, and the measurement results are shown in Table 6.

strain Composition Amount (mg) 5g 1 g 0g clear zone size (mm) S. aureus 20 8 0.1 Ecoli 25 5 0.2 P. aeruginosa 22 4 One

As a result of the measurement, as shown in Table 6, the size of the clear zone increased according to the amount of the composition of the present invention, and it was confirmed that there was an antibacterial activity against Gram-positive bacteria, Gram-negative bacteria and Pseudomonas aeruginosa.

<Experimental Example 3> Confirmation of skin and fur improvement effect of the composition

The mixture composition was sprayed on the non-inflammatory skin of 3 experimental dogs (2-3 year old female beagle) 3 times a day at 6 hour intervals for 3 days, and then oil and moisture were measured. As a comparative example, the oil and moisture content of three experimental dogs that did not spray the composition were measured. In order to make the other conditions the same, the amount of feed eaten and the amount of water supplied were the same. Measurements were taken on the 4th day after spraying on the skin, and the measurement results are shown in Table 7.

experimental dog Moisture content (%) Oil content (%) composition spray group lab dog 1 25.4 10.1 lab dog 2 24.8 12.1 lab dog 3 26.0 10.3 composition spray group lab dog 1 10.5 6.4 lab dog 2 11.0 5.5 lab dog 3 8.9 6.2

As shown in Table 7, it was confirmed that the oil and moisture content of the test dogs increased in the group sprayed with the composition of the present invention, indicating an improvement effect on skin and fur.

Claims (11)

It contains golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan as active ingredients, and the golden extract, cypress leaf extract, rosemary extract and ginseng extract are water, ethanol or these A composition for improving the skin and fur of companion animals, which is an extract extracted through a mixed solution of
delete According to claim 1,
The cocoon extract is an extract extracted through water, ethanol or a mixed solution thereof, a composition for improving the skin and fur of companion animals. According to claim 1,
The collagen hydrolyzate is a cow skin collagen hydrolyzate, a composition for improving skin and fur of companion animals. According to claim 1,
The beta-glucan is a composition for improving the skin and fur of companion animals, which is extracted from chima pepper. According to claim 1,
The beta-glucan is beta-1,6-branched-beta-1,3-glucan, a composition for improving skin and fur of companion animals. According to claim 1,
The golden extract, cypress leaf extract, rosemary extract, ginseng extract, cocoon extract, collagen hydrolyzate, and beta-glucan are each 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, 0.5 to 2 parts by weight, 0.5 based on the weight of the golden extract to 2 parts by weight, 0.5 to 2 parts by weight, and 0.8 to 3.5 parts by weight, a composition for improving skin and fur of companion animals. According to claim 1,
The composition has anti-inflammatory or antibacterial activity, a composition for improving the skin and fur of companion animals. According to claim 1,
The composition is to increase the oil and moisture content of the skin and fur, a composition for improving the skin and fur of companion animals. A cosmetic composition for companion animals comprising the composition for improving skin and fur of companion animals according to any one of claims 1 and 3 to 9. 11. The method of claim 10,
In the cosmetic composition, the composition for improving the skin and fur of companion animals is 1.0 to 10.0 parts by weight based on 100 parts by weight of the cosmetic composition, the cosmetic composition.