KR102507175B1 - Antimicrobial cosmetic composition comprising Salvia Officinalis extract, Caesalpinia Sappan Bark Extract, Houttuynia cordata Extract, Rosmarinus Officinalis Extract, Allium scorodorpasum Extract, and Coptis Chinensis Root Extract as active ingredients - Google Patents

Abstract

It relates to an antibacterial cosmetic composition comprising sage extract, somok extract, eoseongcho extract, rosemary extract, garlic extract, and barberry extract as active ingredients. An antimicrobial cosmetic composition comprising sage extract, somok extract, houttuynia cordata extract, rosemary extract, garlic extract, and barberry extract as active ingredients according to one aspect effectively suppresses the proliferation of bacteria represented by skin flora, thereby providing excellent antibacterial activity. Since it represents a hand sanitizer, it can be widely used as an antibacterial cosmetic composition. In particular, since it contains natural ingredients as its main ingredients, it has excellent antioxidant, anti-inflammatory, elasticity and whitening activities while minimizing skin irritation, and even shows skin improvement effects, so it can exhibit the same sterilization effect as conventional antibacterial compositions.

Description

Antimicrobial cosmetic composition comprising Salvia Officinalis extract, Caesalpinia Sappan Bark Extract, Houttuynia cordata Extract, Rosmarinus Officinalis Extract, Allium scorodorpasum Extract, and Coptis Chinensis Root Extract as active ingredients}

It relates to an antibacterial cosmetic composition comprising sage extract, somok extract, eoseongcho extract, rosemary extract, garlic extract, and barberry extract as active ingredients and a method for preparing the same.

In order to improve personal safety and hygiene from diseases, it is particularly important to remove germs and viruses from hands, and accordingly, many people have recently used antibacterial compositions including hand sanitizers. In particular, when it is difficult to wash hands immediately or there is a risk of infection with pathogens, the use of hand sanitizer is essential.

In particular, as people's interest in personal hygiene such as hand disinfection to prevent food poisoning and infection increases, the demand for antibacterial cosmetic compositions including hand sanitizers increases, and the need for the development of new products in related industries is increasing day by day. In particular, when it is difficult to wash hands immediately or when there is a risk of infection by external pathogens, the use of hand sanitizers is actively recommended, and demand for them is expected to increase.

These antibacterial cosmetic compositions are used to prevent infection of pathogens by rubbing on hands instead of hand washing with water and soap, and their forms are classified into gel type and liquid type. Currently, antibacterial compositions sold as personal hygiene products are prepared based on alcohol, and other chemical additives are added to enhance moisturizing properties. It is made based on alcohol and dries again at an early point after application, so the durability of moisturizing is not excellent, and chemical additives are used. It is a situation that needs development, but is still incomplete.

One aspect provides an antibacterial cosmetic composition and an antibacterial composition for external application for skin containing sage extract, somok extract, eoseongcho extract, rosemary extract, garlic extract, and barberry extract as active ingredients.

Another aspect provides a method for preparing the antibacterial cosmetic composition.

One aspect is Sage Extract ( Salvia Officinalis (Sage) Leaf Extract), Caesalpinia Sappan Bark Extract, Houttuynia cordata Extract, Rosemary Extract ( Rosmarinus Officinalis (Rosemary) Extract), Garlic Extract ( Allium scorodorpasum Extract) , And it provides an antibacterial cosmetic composition and an antibacterial composition for external application for skin containing Coptis Chinensis Root Extract as an active ingredient.

The sage ( Salvia Officinalis (Sage)) is also known as medicinal salvia, and for medicinal purposes, boiled leaves are known to be used for sore throat and gastroenteritis.

The small order ( Caesalpinia Sappan L. ) is known as a deciduous shrub of the leguminous family and is native to Southeast Asia. In particular, the wood of somok is known to be used as a medicinal material in oriental medicine because it has various active ingredients.

The eoseongcho ( Houttuynia cordata ) is a perennial plant of more than 300 and is known as the only species constituting the monotype yakmomil genus, and is called by names such as Chinese herbal medicine and herbal medicine.

The rosemary ( Rosmarinus Officinalis (Rosemary)) is a perennial plant with needle-like leaves and belongs to the same family as mint. It is known that rosemary has anti-aging, anti-inflammatory, elastic recovery, anti-wrinkle, alleviating age spots, and strengthening skin barrier functions.

The garlic ( Allium scorodorpasum ) is a perennial herb of the Amaryllidaceae family Allium family. It has been cultivated for food and medicinal purposes for a long time.

The Coptis Chinensis Root is native to China and is also cultivated in Korea, Japan, and China for herbal medicine. It is known to grow in eczema soil in the shade of a mountain forest.

The inventors of the present invention, in the case of a cosmetic composition containing the above sage extract, somok extract, eoseongcho extract, rosemary extract, garlic extract, and barberry extract as active ingredients, specifically in a specific mixing ratio or weight ratio, It was confirmed that it can be used as an antibacterial cosmetic composition by effectively inhibiting the growth of the antibacterial agent and exhibiting excellent antibacterial activity. In addition, it was specifically confirmed that the composition exhibited excellent antioxidant, anti-inflammatory, elasticity and whitening activities, and even a skin improvement effect.

In this specification, the term "cosmetic composition" means an article used on the human body to clean and beautify the human body to add charm, brighten the appearance, or maintain or promote the health of skin and hair. The cosmetic composition may be for antibacterial purposes.

The skin flora is, for example, Escherichia coli ( E. coli ), Candida albicans ( Candida albicans ), Bacillus subtilis ( Bacillus subtilis ), Staphylococcus aureus ( Staphylococcus aureus ), Aspergillus niger ( Aspergillus niger ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), Escherichia coli ( Escherichia coli ), Micrococcus lutues ( Micrococcus lutues ), Klebsiella new moniee ( Klebsiella pnemoniae ), and Staphylococcus epidermidis ( Staphylococcus epidermidis ) It may be one or more selected from the group consisting of.

The sage extract, somok extract, houttuynia cordata extract, rosemary extract, garlic extract, and barberry extract may be extracts extracted by a solvent from the whole or a part of the aerial part of the tree, or a material derived therefrom, respectively. The part may be a tree stem, root, leaf, flower, petal, seed (seed), fruit flesh, fruit peel, or fruit. Whole trees, parts thereof, or materials derived therefrom used for extraction may be ground or minced or suitably dried.

The antibacterial cosmetic composition is, for example, sage extract, somok extract, eoseongcho extract, rosemary extract, garlic extract and barberry extract, 1 to 3: 0.5 to 2: 1 to 3: 1 to 3: 1 to 3: 1 of Blending ratio (weight ratio), 1 to 2:0.5 to 1:1 to 2:1 to 2:1 to 2:1 blending ratio (weight ratio), 1:1:1:1:1:1 or 2:0.5:1: It may be included in a mixing ratio (weight ratio) of 1:1:1.

Sage extract, Somok extract, Houttuynia cordata extract, rosemary extract, garlic extract, and barberry extract, which are active ingredients included in the antibacterial cosmetic composition, are, for example, 0.001 to 35% by weight, 0.001 to 32.5% by weight, 0.001% by weight, based on the total weight of the composition. to 30% by weight, 0.01 to 32.5% by weight, 0.01 to 30% by weight, 0.1 to 32.5% by weight, 0.1 to 30% by weight, 1 to 32.5% by weight, or 1 to 30% by weight. In the composition, the active ingredient of the composition including sage extract, somok extract, houttuynia cordata extract, rosemary extract, garlic extract, and barberry extract, when the content thereof is less than 0.001% by weight, effectively inhibits the growth of bacteria and achieves excellent antibacterial activity. It is difficult to do, and if it exceeds 35% by weight, formulation is difficult and the feeling of use may be reduced.

The extract contained in the antibacterial cosmetic composition may be extracted using a hydrophilic solvent. The hydrophilic solvent may be water, C ₁ to C ₁₀ monohydric, dihydric, and trihydric alcohols, or mixtures thereof. The alcohol may be a compound having at least one -OH group of C ₁ to C ₁₀ , C ₁ to C ₆ or C ₁ to C ₄ . The alcohol may be methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, isobutanol, tert-butanol, butylene glycol, or mixtures thereof. In one embodiment, the extraction solvent of the jujube tree root extract may be water, a C ₁ to C ₅ monohydric alcohol, a C ₁ to C ₅ dihydric alcohol, or a mixed solvent thereof. In one embodiment, the extraction solvent of the jujube tree root extract may be butylene glycol.

The extract contained in the antibacterial cosmetic composition has a concentration of polyhydric alcohol, for example, about 1 to 100 (w / w)%, for example, 1 to 99.5 (w / w)%, 5 to 90 (w / w) )%, 5 to 85 (w / w)%, 5 to 80 (w / w)%, 5 to 75 (w / w)%, 5 to 70 (w / w)%, 5 to 65 (w / w) )%, 5 to 60 (w / w)%, 5 to 55 (w / w)%, 5 to 50 (w / w)%, 5 to 45 (w / w)%, 5 to 40 (w / w) )%, or 5 to 35 (w/w)%, or 10 to 30 (w/w)%.

The extraction may further include a process of separating the plant residue and the extract by a known method such as filtration. The extraction may also include a step of removing the solvent from the obtained extract by a known method such as concentration under reduced pressure. The extraction may also include preparing a dried extract by drying the obtained extract, such as nitrogen drying or freeze drying. The extract may be in the form of a concentrated solution, or after being dried and then re-solvated with an appropriate solvent such as DMSO, or freeze-dried and powdered.

The composition may further include a crude extract, a fraction, or a combination thereof of the extract. The crude extract is obtained by contacting the sage, somok, houttuynia cordata, rosemary, garlic, and barberry with an extraction solvent, and refers to one that is not separated as containing specific components. The fraction refers to a material containing a specific component separated from the crude extract. The separation may be separation using an organic solvent, chromatography or filtration. The chromatography may be, for example, silica gel column chromatography, Sephadex LH-20 chromatography, ion exchange chromatography, affinity chromatography, size exclusion chromatography, HPLC, or a combination thereof.

The extraction may further include subjecting the crude extract to silica gel column chromatography or Sephadex LH-20 chromatography.

The extract may be extracted by a conventional method in the art, such as heating extraction, pressure extraction, ultrasonic extraction, low-temperature ultrasonic extraction, hot water extraction, reflux cooling extraction, subcritical extraction, or supercritical extraction. The extraction is carried out at 4 ° C to 70 ° C, for example, 4 ° C to 50 ° C, 4 ° C to 40 ° C, 4 ° C to 30 ° C, 10 ° C to 70 ° C, 15 ° C to 70 ° C, 20 ° C to 70 ° C, 4 °C to 50 °C, 10 °C to 50 °C, 15 °C to 40 °C, 15 °C to 35 °C, 15 °C to 30 °C, 15 °C to 25 °C, or 17 °C to 22 °C, or 20 °C. there is. The extraction time may vary depending on the selected temperature and extraction method, for example, 1 hour to 3 days, 1 hour to 2 days, 1 hour to 1 day, 5 hours to 3 days, 5 hours to 2 days, 5 hours to 1 day, 10 hours to 3 days, 15 hours to 2 days, 15 hours to 36 hours, 18 hours to 30 hours, 1 day to 3 days, 1 day to 2 days, 24 hours, 1 hour to 20 hours, 1 hour to 15 hours, 1 hour to 10 hours, 30 minutes to 5 hours, 30 minutes to 3 hours, or 30 minutes to 2 hours. The ultrasonic extraction is, for example, 1 to 200 kHz, 5 to 180 kHz, 10 to 150 kHz, 15 to 120 kHz, 15 to 100 kHz, 18 to 80 kHz, 20 to 60 kHz, 22 to 40 kHz, or 22 to 38 It can be performed with a frequency of kHz. The extraction may be repeated one or more times, for example, 1 to 5 times, 2 to 4 times, or 3 times, and each extraction may be performed in the same way or in a different way. Specifically, for example, each sage, somok, eoseongcho, rosemary, garlic, and barberry extract were added with 1 to 15 times the weight of the extraction solvent compared to each raw material, and a frequency of 20 to 40 kHz for 30 minutes to 3 hours at 15 to 25 ° C. It may be obtained by low-temperature ultrasonic treatment, and more specifically, the low-temperature ultrasonic treatment may be performed at a temperature of 17 to 22 ° C. for 30 minutes to 2 hours at a frequency of 22 to 38 kHz.

The antibacterial cosmetic composition is lotion (skin lotion), toner, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, hand cleanser, foundation, Essences, nutritional essences, packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, suspensions, gels, powders, pastes, mask packs, and sheets may be prepared. Compositions of such formulations may be prepared according to conventional methods in the art. The blending amount of the additional ingredients such as the moisturizer can be easily selected by those skilled in the art within a range not impairing the objects and effects of the present invention, and the blending amount is 0.001 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. weight percent.

The cosmetic composition may further include functional additives and ingredients included in general cosmetic compositions in addition to the active ingredients disclosed herein, and commonly used purified water, thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, A flavoring agent or a combination thereof may be further included. The cosmetic composition may further include, for example, purified water, a thickener, a preservative, a stabilizer, a solubilizer, a surfactant, a carrier, a flavoring agent, or a combination thereof. In addition, the functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts. Ingredients other than those included include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, cooling agents, antiperspirants, purified water and the like.

One skilled in the art may select any additional ingredients and/or amounts thereof such that the beneficial properties of the compositions according to the present disclosure are not adversely affected or substantially unaffected by the anticipated addition. The compounding amount of the additional components can be easily selected by those skilled in the art within a range that does not impair the objects and effects of the present invention, and the compounding amount is about 0.001 to about 30% by weight, specifically about 0.01 to about 30% by weight based on the total weight of the composition. 25% by weight.

In addition, in the cosmetic composition of each formulation, appropriate components may be selected and blended according to the cosmetic formulation or purpose of use. Since the formulation components and methods may follow conventional techniques, detailed descriptions thereof are omitted herein.

The composition for external application for skin for antibacterial use includes, for example, sage extract, somok extract, eoseongcho extract, rosemary extract, garlic extract and barberry extract, 0.5 to 5: 0.5 to 5: 0.5 to 5: 0.5 to 3: 0.5 to 3: 1 compounding ratio (weight ratio), 1 to 4: 1 to 4: 1 to 1.5: 1 to 2: 1 to 2: 1 compounding ratio (weight ratio), 1: 1: 1: 1: 1: 1 compounding ratio (weight ratio), 3 : 3 : 1 : 2 : 2 : 1 mixing ratio (weight ratio), 4 : 4 : 1 : 1 : 1 : 1 mixing ratio (weight ratio), 1.5 : 1.5 : 0.5 : 1 : 1 : 0.5 mixing ratio (weight ratio), or 2 : 2: 0.5: 0.5: 0.5: may be included in a compounding ratio (weight ratio) of 0.5.

The active ingredient included in the composition for external application for skin according to one aspect may be included in, for example, 0.001 to 35% by weight, 0.01 to 32.5% by weight, or 0.1 to 32.5% by weight, or 0.1 to 30% by weight based on the total weight of the composition. .

The external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed. The external skin preparations include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridin, and calin. Hot-water extracts of fruits, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts and other drugs, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can also be mix|blended suitably.

The skin includes all skin areas of the body, including the face, hands, arms, legs, feet, chest, stomach, back, buttocks, and scalp.

The compositions of one aspect can be applied, administered, and applied to a subject in need of antibacterial and disinfecting. The "individual" means an object requiring antibacterial and disinfection, and more specifically, includes all mammals such as humans or non-human primates, mice, dogs, cats, horses, and cows. .

As used herein, the terms "applying", "administering", and "applying" are used interchangeably and result in at least partial localization of a composition according to an embodiment to a desired site, or route of administration. It may mean disposing of a composition according to an embodiment into an object by.

In addition, the composition may be a quasi-drug composition for skin antibacterial having antibacterial activity against skin flora.

The term “quasi-drugs” refers to textiles, rubber products, or similar products used for the purpose of treating, mitigating, treating or preventing human or animal diseases, weak or non-directly acting on the human body, and not instruments or machines. Products used for the purpose of diagnosing, treating, mitigating, treating, or preventing human or animal conditions or diseases, which fall under one of the following categories: It refers to items other than instruments, machines, or devices, and items other than instruments, machines, or devices among items used for the purpose of pharmacologically affecting the structure and function of humans or animals, including external skin products and personal hygiene products. can include

The composition may include the extract in an effective amount or as an active ingredient. The effective amount can be appropriately selected according to the individual. The severity of the disease or condition, the subject's age, weight, health, sex, the subject's sensitivity to the extract, the administration time, administration route and excretion rate, administration period, factors including other compositions combined or used simultaneously with the composition, and other It may be determined according to factors well known in the physiological or medical fields.

Another aspect is drying and cutting sage, somok, eoseongcho, rosemary, garlic and barberry; Extracting the cut sage, somok, eoseongcho, rosemary, garlic, and barberry with water, C ₁ to C ₅ monohydric alcohol, C ₁ to C ₅ dihydric alcohol, or a mixed solvent thereof; And it provides a method for producing an antibacterial cosmetic composition comprising the step of mixing the obtained extract.

The step of extracting the extract may be extracted by a conventional method in the art, such as warming extraction, pressure extraction, ultrasonic extraction, low-temperature ultrasonic extraction, hot water extraction, reflux cooling extraction, subcritical extraction, or supercritical extraction. can be extracted through low-temperature ultrasonic extraction. The extraction is carried out at 4 ° C to 70 ° C, for example, 4 ° C to 50 ° C, 4 ° C to 40 ° C, 4 ° C to 30 ° C, 10 ° C to 70 ° C, 15 ° C to 70 ° C, 20 ° C to 70 ° C, 4 °C to 50 °C, 10 °C to 50 °C, 15 °C to 40 °C, 15 °C to 35 °C, 15 °C to 30 °C, 15 °C to 25 °C, or 17 °C to 22 °C, or 20 °C. there is. The extraction time may vary depending on the selected temperature and extraction method, for example, 1 hour to 3 days, 1 hour to 2 days, 1 hour to 1 day, 5 hours to 3 days, 5 hours to 2 days, 5 hours to 1 day, 10 hours to 3 days, 15 hours to 2 days, 15 hours to 36 hours, 18 hours to 30 hours, 1 day to 3 days, 1 day to 2 days, 24 hours, 1 hour to 20 hours, 1 hour to 15 hours, 1 hour to 10 hours, 30 minutes to 5 hours, 30 minutes to 3 hours, or 30 minutes to 2 hours. The ultrasonic extraction is, for example, 1 to 200 kHz, 5 to 180 kHz, 10 to 150 kHz, 15 to 120 kHz, 15 to 100 kHz, 18 to 80 kHz, 20 to 60 kHz, 22 to 40 kHz, or 22 to 38 It can be performed with a frequency of kHz. The extraction may be repeated one or more times, for example, 1 to 5 times, 2 to 4 times, or 3 times, and each extraction may be performed in the same method or in a different method. Specifically, for example, each sage, somok, eoseongcho, rosemary, garlic, and barberry extract were added with 1 to 15 times the weight of the extraction solvent compared to each raw material, and a frequency of 20 to 40 kHz for 30 minutes to 3 hours at 15 to 25 ° C. It may be obtained by low-temperature ultrasonic treatment, and more specifically, the low-temperature ultrasonic treatment may be performed at a temperature of 17 to 22 ° C. for 30 minutes to 2 hours at a frequency of 22 to 38 kHz.

Other objects and advantages of this application will become more apparent from the following detailed description taken in conjunction with the appended claims and drawings. Any person skilled in the art of this application or a similar technical field can sufficiently recognize and infer the content not described in this specification, and thus the description thereof will be omitted.

Each description and embodiment disclosed in this application can also be applied to each other description and embodiment. That is, all combinations of various elements disclosed in this application fall within the scope of this application. In addition, the scope of the present application is not to be construed as being limited by the specific descriptions described below.

An antimicrobial cosmetic composition comprising sage extract, somok extract, houttuynia cordata extract, rosemary extract, garlic extract, and barberry extract as active ingredients according to one aspect effectively suppresses the proliferation of bacteria represented by skin flora, thereby providing excellent antibacterial activity. Since it represents a hand sanitizer, it can be widely used as an antibacterial cosmetic composition. In particular, since it contains natural ingredients as its main ingredients, it has excellent antioxidant, anti-inflammatory, elasticity and whitening activities while minimizing skin irritation, and even shows skin improvement effects, so it can exhibit the same sterilization effect as conventional antibacterial compositions.

1 is a view confirming the results shown by comparing the antibacterial activity shown when the compositions of Comparative Example and Examples 1 to 3 were treated with positive and negative control groups in Escherichia coli strain and Staphylococcus orius strain (FIG. 1B).
Figure 2 is a diagram relating to the results of confirming the antibacterial activity of the hand sanitizer in Escherichia coli (Fig. 2A) and Staphylococcus orius (Fig. 2).
Figure 3 is a schematic diagram of a method for preparing an example composition.

It will be described in more detail through the following examples. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

Comparative Example 1: Preparation of sage extract

Take 25 g of raw material (Germany) for Sage ( Salvia Officinalis (Sage), dry it, cut it into 1 to 5 cm pieces, put it in a container, and use 150 mL of 30 wt% butylene glycol as an extraction solvent at a temperature of 85 ° C. 5% sage extract was prepared by hot water extraction or ultrasonic extraction for 2 hours, and then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Comparative Example 2: Preparation of Somok Extract

Take 25g of raw material (Indonesia) for Caesalpinia Sappan L. , dry it, cut it into 1 to 5cm pieces, put it in a container, and use 150mL of 30% by weight of butylene glycol as an extraction solvent at a temperature of 85 ° C. A 5% sorghum extract was prepared by hot water extraction or ultrasonic extraction for 2 hours. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Comparative Example 3: Preparation of Eoseongcho extract

Take 25g of raw material (domestic) for Houttuynia cordata , dry it, cut it into 1 to 5cm pieces, put it in a container, and 2 hours at a temperature of 85 ℃ with 150mL of 30% by weight butylene glycol as an extraction solvent 5% Eoseongcho extract was prepared by hot water extraction or ultrasonic extraction. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Comparative Example 4: Preparation of rosemary extract

Take 25g of raw material (Jeju Plant Resources Research Institute) for rosemary ( Rosmarinus Officinalis (Rosemary)), dry it, cut it into pieces of 1 to 5cm, put it in a container, and mix it with 150mL of 30% by weight of butylene glycol as an extraction solvent. A 5% rosemary extract was prepared by hot water extraction or ultrasonic extraction at a temperature of °C for 2 hours. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Comparative Example 5: Preparation of Garlic Extract

Take 25g of raw material (domestic) for garlic ( Allium scorodorpasum ), dry it, cut it into pieces of 1 to 5cm, put it in a container, and use 150mL of 30% by weight of butylene glycol as an extraction solvent for 2 hours at a temperature of 85 ° C. 5% garlic extract was prepared by hot water extraction or ultrasonic extraction. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Comparative Example 6: Preparation of Barberry Extract

Take 25g of raw material (China) for Coptis Chinensis Root, dry it, cut it into pieces of 1 to 5cm, put it in a container, and use 150mL of 30% by weight of butylene glycol as an extraction solvent at a temperature of 85 ° C. A 5% Barberry extract was prepared by hot water extraction or ultrasonic extraction for a period of time. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Examples 1 to 3: Preparation of mixed extracts of sage, somok, eoseongcho, rosemary, garlic, and barberry

Sage obtained from each extract of sage, barberry, somok, garlic, rosemary and eoseongcho obtained in Comparative Examples 1-6: sage: barberry: garlic: rosemary: somok: eoseongcho blending ratio (weight ratio) = 1: 1: 1: 1: 1: 1 (Example 1), 3: 3: 1: 2: 2: 1 (Example 2), 4: 4: 1: 1: 1: 1 (Example 3) were mixed and used in the following experimental examples. .

Experimental Example 1: Confirmation of skin improvement effect of compositions of Examples and Comparative Examples

1.1 Confirmation of skin improvement effect by extraction method

In the case of preparing the rosemary, garlic, sage, somok, houttuynia cordata, and barberry extracts of Comparative Examples 1-6, the extraction method is used to determine whether the skin improvement effect, specifically antioxidant, anti-inflammatory, elasticity, and whitening effect can be changed. An experiment was conducted. Specifically, an experiment was conducted to confirm the DPPH free radical scavenging activity to confirm the antioxidant activity, and to confirm the anti-inflammatory effect, it was confirmed whether the production of nitric oxide could be inhibited, and the activity for skin elasticity In order to find out, an experiment to confirm the elastase inhibitory activity was performed as a skin wrinkle improvement experiment, and an experiment to confirm the tyrosinase inhibitory activity was performed to confirm the activity for skin whitening, Table 1 shows the results of the compositions prepared by hot water extraction of Comparative Examples 1-6, and Table 2 shows the results of the compositions prepared by ultrasonic extraction.

hot water extract Efficacy (%) Rosemary garlic sage joiner Eoseongcho goldthread Antioxidant (DPPH) 12.2 5.3 41.0 2.3 4.9 12.5 Anti-inflammatory (NO) 84.5 1.3 X 10.3 X 9.0 Elastase 6.0 4.0 12.9 8.9 20.6 3.0 Whitening (Tyrosinase) 2.5 2.2 X 1.6 5.4 X

ultrasonic extract Efficacy (%) Rosemary garlic sage joiner Eoseongcho goldthread Antioxidant (DPPH) 18.7 15.5 49.5 6.3 8.5 5.5 Anti-inflammatory (NO) 86.5 8.7 25.3 25.3 X 17.3 Elastase 10.1 8.9 13.5 10.5 35.3 3.7 Whitening (Tyrosinase) 5.6 5.2 X 4.3 4.8 X

As confirmed in Tables 1 and 2, it was confirmed that each of the extract compositions extracted through ultrasonic extraction was superior to the hot water extract in antioxidant, anti-inflammatory, elasticity and whitening effects on skin improvement. In addition, when extracted with hot water, it was confirmed that some extracts did not show any skin improvement effect, and it was confirmed that the skin improvement effect occurred when ultrasonically extracted. Accordingly, in the following examples, the skin improvement ability was confirmed using the ultrasonic extract.

1.2 Confirmation of skin improvement effect by optimal mixing ratio

An experiment was performed to confirm the skin improvement effect of the compositions of Examples 1 to 3 containing sage, barberry, garlic, rosemary, somok, and houttuynia cordata in an optimal mixing ratio compared to the comparative composition, and these are shown in Table 3. The skin improvement effect was performed under the same conditions as in Experimental Example 1.1, and a commercially available hand sanitizer was used as a control group.

Efficacy (%) Example 1 Example 2 Example 3 Hand sanitizer Antioxidant (DPPH) 39.5 38.4 40.1 X Anti-inflammatory (NO) 38.6 32.9 45.4 X Elastase 15.3 11.1 8.9 X Whitening (Tyrosinase) 3.5 2.3 2.1 X

As confirmed in Table 3, it was confirmed that the hand sanitizer composition used as the control example did not show antioxidant, anti-inflammatory, elasticity and whitening activities, but the compositions of Examples 1 to 3 showed antioxidant, anti-inflammatory, elasticity and whitening activities overall. It was confirmed that the activity was excellent. Therefore, it was confirmed that the compositions of Examples 1-3 exhibited excellent antioxidant, anti-inflammatory, elasticity, and whitening activities, and thus could be usefully utilized, compared to Comparative Example, in which only some of the active effects were shown as skin improvement effects.

Experimental Example 2: Confirmation of antibacterial activity of compositions of Examples and Comparative Examples against skin flora

The antibacterial activity of the compositions of Comparative Examples 1, 2, and 6 and Examples 1 to 3 against Escherichia coli (E. coli ) and Staphylococcus aureus , which is a skin flora, was measured by a disc diffusion method (Disc diffusion assay, clear zone). Specifically, after activating two stock strains (E.coli, S.aureus) in a liquid medium, the primary culture was performed. Thereafter, the positive control was set as 0.025% of tetracycline, an antibacterial substance, and the negative control was set as BG 30%, and a sample thereof was prepared and the primary culture medium smeared on a solid medium suitable for each strain condition (sterilized using a cotton swab) and then dried. Thereafter, sterilized Paper disc 8mm Thin is attached so that the flat side touches the solid medium, and the positive control, negative control, commercially available hand sanitizer composition (ethanol 62%, Korea Life Science Co., Ltd.) and samples are dispensed on each 20ul Paper disc, It was cultured at 37 ° C for 24 H, and the antibacterial activity was confirmed by measuring the final clear zone diameter. The sample was subjected to antibacterial experiments of the hot water and ultrasonic compositions of Comparative Examples 1-6, and the results are shown in Table 4. Then, the positive and negative control groups and the antibacterial activity of the compositions of Examples 1-3 were tested against Escherichia coli (Fig. 1A), Staphylococcus orius (FIG. 1B) and the results summarized by analyzing their antibacterial activity are shown in Table 5, and the antibacterial activity of other commercially available hand sanitizers was confirmed in Escherichia coli and Staphylococcus orius. 2A and 2B.

positive control sage
(Comparative Example 1) goldthread
(Comparative Example 6) joiner
(Comparative Example 2) hydrothermal Escherichia coli 17 9 9 9 Staphylococcus Orius 17 11 11 10 ultrasonic wave Escherichia coli 17 12 12 10 Staphylococcus Orius 17 14 14 11

As confirmed in Table 4, it was confirmed that the compositions of Comparative Examples 1, 2, and 6, which consisted of hot water extraction versus ultrasonic extraction, exhibited antibacterial activity in both Escherichia coli and Staphylococcus orius, and in particular, antibacterial activity similar to that of the positive control group. activity was confirmed.

positive control
(One) negative control group
(2) Example 1(3) Example 2(4) Example 3(5) Escherichia coli 13 X X X 10 Staphylococcus Orius 26 X 16 16 20

As confirmed in Table 5 and FIGS. 1A and 1B, it was confirmed that all of Examples 1 to 3 showed antibacterial activity in the skin flora, Staphylococcus orius strain. Even in Escherichia coli, it was confirmed that the composition of Example 3 exhibited the same level of antimicrobial activity as that of the positive control group. It was confirmed that such antibacterial activity is a result different from that in which the hand sanitizer stock solution and the 50% dilution of the hand sanitizer as confirmed in FIG. 2 do not exhibit antibacterial activity at all against the two strains.

Therefore, in the case of Example Compositions 1 to 3 containing these in a specific mixing ratio, compared to Comparative Examples 1 to 6, each of which is a sage extract, barberry extract, sorghum extract, garlic extract, rosemary extract, and houttuynia cordata extract, the skin improving activity is shown and at the same time the skin It was confirmed that it can be usefully used as an antibacterial composition because it exhibits excellent antibacterial activity against all of the normal flora.

Claims (10)

Sage Extract ( Salvia Officinalis (Sage) Leaf Extract), Coptis Chinensis Root Extract, Caesalpinia Sappan Bark Extract, Garlic Extract ( Allium scorodorpasum Extract), Rosemary Extract ( Rosmarinus Officinalis (Rosemary) Extract) and Hoseongcho An antibacterial cosmetic composition comprising an extract ( Houttuynia cordata Extract) as an active ingredient,
The composition comprises sage extract, barberry extract, somok extract, garlic extract, rosemary extract and eoseongcho extract in a compounding ratio (weight ratio) of 0.5 to 5: 0.5 to 5: 0.5 to 5: 0.5 to 3: 0.5 to 3: 1 composition. delete The cosmetic composition according to claim 1, wherein the active ingredient is included in an amount of 0.001 to 35% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the extraction solvent of the extract is extracted with water, C ₁ to C ₅ monohydric alcohol, C ₁ to C ₅ dihydric alcohol, or a mixed solvent thereof. The cosmetic composition according to claim 4, wherein the dihydric alcohol of the extract is butylene glycol. The cosmetic composition according to claim 1, wherein the extract is extracted by an extraction solvent at a concentration of 1 to 100 (w/w)%. The method of claim 1, lotion, toner, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, hand sanitizer, foundation, essence, nutrient essence, A cosmetic composition of at least one formulation selected from the group consisting of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, suspensions, gels, powders, pastes, mask packs, and sheets. The cosmetic composition according to claim 1, wherein the composition further comprises purified water, a thickener, a preservative, a stabilizer, a solubilizer, a surfactant, a carrier, a flavoring agent, or a combination thereof. Sage Extract ( Salvia Officinalis (Sage) Leaf Extract), Caesalpinia Sappan Bark Extract, Houttuynia cordata Extract, Rosemary Extract ( Rosmarinus Officinalis (Rosemary) Extract), Garlic Extract ( Allium scorodorpasum Extract), and Bark Lily An antibacterial composition for external application for skin containing Coptis Chinensis Root Extract as an active ingredient,
The composition comprises sage extract, barberry extract, somok extract, garlic extract, rosemary extract and eoseongcho extract in a compounding ratio (weight ratio) of 0.5 to 5: 0.5 to 5: 0.5 to 5: 0.5 to 3: 0.5 to 3: 1 composition. Drying and cutting sage, somok, eoseongcho, rosemary, garlic and barberry;
Extracting the cut sage, somok, eoseongcho, rosemary, garlic, and barberry with water, C ₁ to C ₅ monohydric alcohol, C ₁ to C ₅ dihydric alcohol, or a mixed solvent thereof; and
Mixing the obtained sage extract, barberry extract, somok extract, garlic extract, rosemary extract and eoseongcho extract in a compounding ratio (weight ratio) of 0.5 to 5: 0.5 to 5: 0.5 to 5: 0.5 to 3: 0.5 to 3: 1 Method for producing an antibacterial cosmetic composition comprising the step.

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