KR102327236B1 - Compositions for Anti-microbial and Deodorization Effect Comprising Complex Extract of Plants - Google Patents

Abstract

The present invention relates to an antibacterial and deodorizing composition comprising a golden subcritical complex extract as an active ingredient, and more specifically, to a subcritical complex extract of gold, cedar, cypress, cypress, bamboo sundae, and fir tree as an active ingredient. It relates to an antibacterial and deodorizing composition.
The antibacterial and deodorant composition of the present invention effectively inhibits the growth of harmful bacteria and exhibits an excellent antibacterial effect, and exhibits an excellent effect on deodorization through removal of ammonia gas and inhibition of isovalic acid production. It can be usefully used for

Description

Compositions for Anti-microbial and Deodorization Effect Comprising Complex Extract of Plants

The present invention relates to an antibacterial and deodorizing composition comprising a plant complex extract as an active ingredient, and more specifically, to an antibacterial and deodorizing composition comprising a subcritical complex extract of gold, cedar, cypress, cypress, bamboo sundae, and fir as an active ingredient. And it relates to a deodorizing composition.

Although many air fresheners with antibacterial and deodorant functions are available on the market, most of these air fresheners use synthetic raw materials to cause respiratory problems or irritate the skin, causing problems for people with sensitive skin. . In particular, components such as formaldehyde, methanol, naphthalene, and benzene are known carcinogens and are avoided by consumers. Accordingly, studies are underway to use natural substances such as phytoncide synthesized in nature for antibacterial and deodorizing fields.

Phytoncide produced by conifers such as cedar, cypress, and fir is a self-defense substance of plants. It is a substance emitted or secreted by plants to resist pathogens, pests, and fungi. It contains sterilizing and insecticidal ingredients. The constituent substances of phytoncide are organic compounds composed of phenolic compounds, alkaloids, glycosides, etc. including terpenes, and are known to have antibacterial, sedative, deodorant, and stress relieving effects. These phytoncides are natural substances, not chemically synthesized substances, are easily absorbed by the human body, and selectively sterilize harmful bacteria to humans. Therefore, various studies such as Patent Publication No. 10-2019-0031983 and Patent Registration No. 10-1966673 are being conducted in order to utilize the active ingredients isolated from conifers for the purpose of antibacterial or deodorization.

However, the antibacterial and deodorant activities of cedar, cypress, and fir extracts are not yet sufficient for industrial use, so they are not used in real life. is the state

Under this background, the present inventors made diligent efforts to find a combination of plant extracts with excellent antibacterial and deodorant activity, and as a result, the combination of subcritical extracts of gold, cedar, cypress, cypress, bamboo sundae, and fir can kill various harmful bacteria It was confirmed that it can be effectively used as an antibacterial agent and a deodorant by effectively inhibiting it, effectively removing ammonia that causes body odor, and inhibiting the production of isovalic acid, and completed the present invention.

Accordingly, the main object of the present invention is to effectively inhibit the growth of harmful bacteria and to provide a composition comprising, as an active ingredient, a subcritical extract of gold, cedar, cypress, cypress, bamboo sundae, and fir, which exhibits excellent deodorizing effect. have.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention is gold ( Scutellaria baicalensis ) subcritical extract, cedar ( Cryptomeria Japonica ) subcritical extract, cypress ( Chamaecyparis ) Obtusa ) Subcritical Extract, Cypress Tree ( Thujopsis Dolabrata ) Subcritical Extract, Bamboo Sundae ( Phyllostachys ) Heterocycla ) subcritical extract, and fir tree ( Abies Sibirica ) Provides an antibacterial and deodorizing composition containing the subcritical extract as an active ingredient.

The present inventors are effective in inhibiting the growth of harmful bacteria, and at the same time, as a result of intensive research efforts to find a combination of effective plant extracts that have a deodorizing effect, gold ( Scutellaria baicalensis ) subcritical extract, cedar ( Cryptomeria Japonica ) subcritical extract, cypress ( Chamaecyparis ) Obtusa ) Subcritical Extract, Cypress Tree ( Thujopsis Dolabrata ) Subcritical Extract, Bamboo Sundae ( Phyllostachys ) Heterocycla ) subcritical extract, and fir tree ( Abies Sibirica ) When the subcritical extract was combined, it was found that the antibacterial and deodorant effects against harmful bacteria were dramatically increased, and there was no problem in safety even when applied to the skin. Confirmed.

The term gold of the present invention ( Scutellaria baicalensis )” means the root of Scutellaria baicalensis Georgi, a perennial herb of the Lamiaceae, and the term “cedar ( Cryptomeria Japonica )” refers to an evergreen tree of the gymnosperm family Lamiaceae, the term “cypress” tree ( Chamaecyparis Obtusa )" means an evergreen arboreous tree of the Arboraceae family , and the term "Bamboo Sundae ( Phyllostachys ) Heterocycla )” means a monocotyledonous bamboo in the genus, and the term “fir tree ( Abies)” Sibirica )” means an evergreen tree of the gymnosperm conifers, subclass Conifers, Pineaceae.

As used herein, the term "extract" refers to an extract obtained by extraction treatment of plants, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc., It includes extracts of all formulations that can be formed using the extract itself and the extract. The extract of the present invention may be prepared and used in the form of a dry powder after extraction.

The subcritical extracts of gold, cedar, cypress, cypress, bamboosundae, and fir trees of the present invention may be extracted from natural, hybrid or mutated plants of gold, cedar, cypress, cypress, bamboosundae, and fir, and , can be extracted from plant tissue culture, and includes extracts extracted from parts such as leaves, stems, roots, flowers, and fruits.

In the present invention, subcritical extraction (SE) is a method of extracting a specific organic material within a short time by using a change in the dielectric constant of a solvent according to temperature and pressure from a solid or semi-solid sample. When water is used as a solvent in the subcritical extraction, the organic solvent used in the separation process can be greatly reduced. The subcritical condition of the present invention refers to a temperature and pressure range below the critical point (critical point: T = 374.2 °C, P = 218.3 atm) in which liquid and gaseous states of water coexist. Water loses its intrinsic polarity as intermolecular hydrogen bonds are broken at a pressure of 50 to 150 atmospheres near the critical point and a temperature of 200 to 300° C., so that the dielectric constant is rapidly reduced. At this time, the dielectric constant of water becomes 2.5 or less, so that it can be used as an extraction solvent for a specific organic material.

Therefore, in the antibacterial and deodorizing composition of the present invention, the subcritical extract is preferably extracted under a temperature condition of 160 to 230 °C and a subcritical condition of 50 to 150 atm, and a temperature condition of 180 to 200 °C and 100 to It is most preferable to extract under subcritical conditions of 130 atmospheres. Under the above conditions, water as a solvent is converted to subcritical water and undergoes intense hydrolysis, and excellent extraction ability is exhibited for gold, cedar, cypress, cypress, bamboo shoots, and fir.

In the antibacterial and deodorizing composition of the present invention, the composition is Escherichia coli KCTC2571, Pseudomonas aeruginosa KCTC2513), Staphylococcus aureus (Staphylococcus aureus KCTC3881), Candida albicans (Candida albicans) , and Aspergillus brasiliensis (Aspergillus brasiliensis KCTC6317) characterized in that it exhibits antibacterial activity. In an embodiment of the present invention, an antibacterial composition against harmful bacteria was prepared by combining the subcritical extracts of gold, cedar, cypress, cypress, bamboo sundae, and fir, and the minimum microbial growth in order to confirm the antibacterial activity of the prepared composition. Inhibitory concentration assessments were performed. As a result, it was confirmed that the combination of six subcritical extracts of gold, cedar, cypress, cypress, bamboosundae, and fir tree had superior antibacterial activity against harmful bacteria compared to a single subcritical extract. In particular, it was confirmed that there was an excellent synergistic effect compared to the combination of the hot water extract or the distilled extract used as a control.

In the antibacterial and deodorizing composition of the present invention, the composition is characterized in that it removes ammonia gas. In an embodiment of the present invention, it was confirmed that the subcritical complex extracts of gold, cedar, cypress, cypress, bamboosundae, and fir had a very excellent ammonia gas deodorization effect, and the subcritical extract combination of 6 components increased It was confirmed that synergistic effect was exerted by the deodorizing effect of ammonia gas.

In addition, in the antibacterial and deodorant composition of the present invention, the composition is characterized in that it inhibits the leucine dehydrogenase enzyme activity to inhibit the production of isovalic acid. In an embodiment of the present invention, the leucine dehydrogenase enzyme activity was measured to be 72% to 95% in the test groups treated with subcritical extracts of plant single raw materials of gold, cedar, cypress, cypress, bamboosundae, and fir. It was found that the enzyme did not effectively inhibit the leucine dehydrogenase enzyme. On the other hand, in the test group treated with the six plant subcritical complex extracts, the leucine dehydrogenase enzyme activity was measured to be 42%, confirming that it was very effective in inhibiting isovalic acid production.

According to another aspect of the present invention, the present invention is gold ( Scutellaria baicalensis ) subcritical extract, cedar ( Cryptomeria Japonica ) subcritical extract, cypress ( Chamaecyparis ) Obtusa ) Subcritical Extract, Cypress Tree ( Thujopsis Dolabrata ) Subcritical Extract, Bamboo Sundae ( Phyllostachys ) Heterocycla ) subcritical extract, and fir tree ( Abies Sibirica ) Provides a cosmetic composition for antibacterial and deodorant containing the subcritical extract as an active ingredient.

The cosmetic composition for antibacterial and deodorant comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboo sundae, and fir as an active ingredient of the present invention exhibits strong growth inhibitory activity against harmful bacteria, removes ammonia gas and Since it has an excellent effect on deodorization through inhibition of isovalic acid production, it can be usefully used as an antibacterial and deodorizing cosmetic composition.

The cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions in addition to the subcritical complex extracts of gold, cedar, cypress, cypress, bamboo sundae, and fir as active ingredients, for example, antioxidants, stabilizers, and dissolving agents. conventional adjuvants such as topical agents, vitamins, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it can be prepared in the form of flexible lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essential oil, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. have.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. are used as carrier components. can be

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tragacanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

Specific examples of the cosmetic of the present invention include feminine cleanser, hand sanitizer, face cream, face wash foam, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisture cream, emulsion, lotion, pack, after-saving cream, anti-sunburn cream , Suntan oil, soap, body shampoo, hair shampoo, hair conditioner, hair treatment, hair conditioner, hair growth, hair cream, hair liquid, set lotion, hair spray, hair bridge, color rinse, color spray, permanent wave solution , press powder, loose powder, eye shadow, hand cream, and lipstick.

According to another aspect of the present invention, the present invention is gold ( Scutellaria baicalensis ) subcritical extract, cedar ( Cryptomeria Japonica ) subcritical extract, cypress ( Chamaecyparis ) Obtusa ) Subcritical Extract, Cypress Tree ( Thujopsis Dolabrata ) Subcritical Extract, Bamboo Sundae ( Phyllostachys ) Heterocycla ) subcritical extract, and fir tree ( Abies Sibirica ) Provides a pharmaceutical composition for antibacterial and deodorant containing the subcritical extract as an active ingredient.

The antibacterial and deodorant composition comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboo sundae, and fir as an active ingredient of the present invention exhibits strong growth inhibitory activity against harmful bacteria, removes ammonia gas, and isolates Since it has an excellent effect on deodorization through inhibition of valic acid production, it can be usefully used as a pharmaceutical composition for antibacterial and deodorizing.

On the other hand, the antibacterial and deodorant pharmaceutical composition comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboo sundae, and fir as an active ingredient of the present invention can be prepared in the form of a pharmaceutically acceptable salt. . Specifically, salts can be formed by addition of acids, for example, inorganic acids (e.g. hydrochloric acid, hydrobromic acid, phosphoric acid, nitric acid, sulfuric acid, etc.), organic carboxylic acids (e.g. acetic acid, trifluoroacetic acid, etc.) acetic acid, propionic acid, maleic acid, succinic acid, malic acid, citric acid, tartaric acid, salicylic acid), and acidic sugars (glucuronic acid, galacturonic acid, gluconic acid, ascorbic acid), acidic polysaccharides such as hyaluronic acid, chondroitin sulfate, arginic acid), organic sulfonic acids including sulfonic acid sugar esters such as chondroitin sulfate (eg, methane, sulfonic acid, p-toluene sulfonic acid), etc. may be added to form salts.

The antibacterial and deodorant pharmaceutical composition comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboosundae, and fir as an active ingredient of the present invention is a substance separated from a natural plant extract, and when administered orally or parenterally, clinically It can be administered orally and can be used in the form of general pharmaceutical formulations.

That is, the antibacterial and deodorant pharmaceutical composition comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboosundae, and fir as an active ingredient of the present invention can be administered in various oral or parenteral formulations. However, in the case of formulation, it is usually prepared using a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, and a surfactant. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, liulinji, glycerogelatin, and the like can be used.

In addition, the antibacterial and deodorant pharmaceutical composition comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboosundae, and fir as an active ingredient of the present invention is a physiological saline solution or organic solvent. It can be used in admixture with a carrier, and to increase stability or absorption, carbohydrates such as glucose, sucrose or dextran, antioxidants such as ascorbic acid or glutathione, chelating agents agents), low molecular weight proteins or other stabilizers may be used as pharmaceuticals.

The effective dose of the pharmaceutical composition for antibacterial and deodorization comprising the subcritical complex extract of gold, cedar, cypress, cypress, bamboosundae, and fir as an active ingredient of the present invention is 0.01 to 10 mg/kg, preferably 0.1 to 1 mg/kg, and may be administered 1 to 3 times a day.

The total effective amount of the pharmaceutical composition or the culture medium of the present invention may be administered to the patient in a single dose in the form of a bolus or by infusion for a relatively short period of time, and multiple doses (multiple does) can be administered by a fractionated treatment protocol that is administered over a long period of time. Since the concentration is determined by considering various factors such as the age and health status of the patient as well as the administration route and number of treatments, the effective dosage of the patient is determined. It will be possible to determine an appropriate effective dosage according to the particular use of the eugenol and gallic acid combination as a pharmaceutical composition.

The features and advantages of the present invention are summarized as follows:

(a) the present invention is gold ( Scutellaria baicalensis ) subcritical extract, cedar ( Cryptomeria Japonica ) subcritical extract, cypress ( Chamaecyparis ) Obtusa ) subcritical extract, Thujopsis Dolabrata ) Subcritical Extract, Bamboo Sundae ( Phyllostachys) Heterocycla ) Subcritical Extract, and Fir ( Abies) Sibirica ) provides an antibacterial and deodorizing composition containing a subcritical extract as an active ingredient.

(b) the antibacterial and deodorizing composition of the present invention is harmful bacteria Escherichia coli KCTC2571, Pseudomonas aeruginosa KCTC2513, Staphylococcus aureus (Staphylococcus aureus KCTC3881), Candida albicans (Candida albicans) KCTC7965), and Aspergillus brasiliensis (Aspergillus brasiliensis KCTC6317) to effectively inhibit the growth of the strain shows an excellent antibacterial effect.

(c) In addition, the composition of the present invention exhibits an excellent effect on deodorization through the removal of ammonia gas and inhibition of isovalic acid production.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and therefore, the scope of the present invention should not be construed as being limited by these examples.

Example 1. Preparation of Subcritical Plant Extract

After grinding 100 g of dried gold, 3 L of water as a solvent using subcritical extraction equipment (Dionex Accelerated Solvent Extractor, ASE Model 100) at a temperature of 180 to 200 ° C and 100 to 130 atm for 8 to 20 minutes After subcritical extraction, the mixture was cooled and filtered with a filter paper having a penetration size of 1.2 μm to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure and dried under reduced pressure to prepare a golden subcritical extraction dry powder (Test Example 1).

Dry powders of cedar leaves, cypress leaves, cypress leaves, bamboo sundae roots, and fir leaves were prepared in the same manner as above, respectively (Test Examples 2 to 6).

[Table 1]

In addition, the six subcritical complex extracts of Test Example 7 were prepared by complex extraction of the six plant components.

For the comparative experiment, after pulverizing 100 g of a mixed raw material mixed in equal amounts of 6 types of plant components, using 3 L of water as a solvent, hot water extraction for 12 hours, cooling, and filtering with filter paper having a 1.2 μm permeation size, 6 types A hot water complex extract was prepared (Comparative Example 1).

In addition, 100 g of a mixed raw material obtained by mixing equal amounts of six plant components was washed with purified water and dried, then pulverized and finely divided to obtain a powder. Each powdered plant material was heated to 100° C., and then water vapor was collected and cooled to prepare six distillation complex extracts (Comparative Example 2).

Example 2. Antibacterial testing of single and complex subcritical extracts

In order to measure the antimicrobial activity of the six subcritical plant extracts of the present invention, the microbial growth minimum inhibitory concentration (MIC) was evaluated for harmful microorganisms.

Harmful microorganisms include Escherichia coli KCTC2571, Pseudomonas aeruginosa KCTC2513, Staphylococcus aureus KCTC3881 strain, the fungi Candida albicans (Candcilida albicans KCTC7965), and Ensis (Aspergillus brasiliensis KCTC6317) was used (KCTC Korea Research Institute of Bioscience and Biotechnology Biological Resources Center).

The medium used for the culture of E. coli was LB medium, and the culture of Pseudomonas aeruginosa and S. aureus strains was NA medium, and Candida albiosis. Cans (C. albicans) and Aspergillus brasiliensis (Aspergillus brasiliensis) strains were used YM medium and PDA medium, respectively. Each bacteria was inoculated by obtaining 2-3 colonies (1×10 ⁷ CFU/mL). It was incubated with the subcritical extract sample prepared in Example 1 for 18 hours. After incubation, the growth of the bacteria was observed with the naked eye and a microscope, and the absorbance was measured at an absorption wavelength of 600 nm.

For MIC measurement, after culturing each bacteria and fungus on a solid agar medium, ^{5 colonies were obtained according to} 1×10 5-6 CFU/mL, diluted in the medium, inoculated, and maintained at 37° C. for 2-3 hours.

The subcritical extract used in this experiment was titrated to a concentration of 2% (mg/ml) and each diluted two-fold. The medium containing each bacteria and subcritical extract was transferred to a 96 well plate, incubated at 37° C. for 16 to 24 hours, and measured with a Multilabel Counter (1420, Perkin Elmer) at 600 nm. The subcritical extract prepared in Example 1 The minimum inhibitory concentration values of them were obtained, and it is shown in [Table 2] below.

[Table 2]

As a result of the minimum inhibitory concentration test for the subcritical extracts of the six plants, the minimum inhibitory concentration values of the subcritical extracts using the plant components of Test Examples 1 to 6 as raw materials were 7 to 17 mg/mL, E for the S. aurues strain. 12 to 17 mg/mL for the .coli strain, 11 to 20 mg/mL for the P. aeruginosa strain, 15 to 30 mg/mL for the C. albicans strain, and 10 to 20 mg/mL for the A. brasiliensis strain. .

However, Test Example 7, which combines six subcritical extracts of gold, cedar, cypress, cypress, bamboosundae, and fir, has increased antibacterial and antibacterial and It could be confirmed that it has an antifungal effect, and the minimum inhibitory concentration of microbial growth reduced by 60% to 525% was measured for all five types of harmful microorganisms, confirming that there is the most excellent synergistic effect.

In addition, it was confirmed that the six subcritical complex extracts of Test Example 7 had very excellent antibacterial and antifungal effects compared to the six types of hot-water complex extracts of Comparative Example 1 or the six types of distillation complex extracts of Comparative Example 2.

[Table 3]

Through the above results, it was confirmed that the combination of six components of golden, cedar, cypress, cypress, bamboosundae, and fir tree exerts an increased antibacterial and antifungal effect to create synergy, and antibacterial through subcritical extraction method. And it was confirmed that the antifungal effect is further enhanced.

Example 4. Measurement of Deodorization Effect on Ammonia Gas

The deodorization effect was measured by a gas detection tube (Gastec Corporation, Japan/GASTEC No. 3M) method based on ammonia gas. The combination composition used to measure the deodorizing effect was prepared by a prescription of 15% ethanol and 83% purified water in 2% (v/v) of the samples of Test Examples 1 to 7 prepared in Example 1. After preparing two 5,000ml chambers, the specimens were dried until they became constant in a dryer capable of maintaining at 110°C, and after cooling in a desiccator, the specimens were immediately mounted in the prepared chambers. After injecting a certain amount of gas to 500ppm each into the chamber for the blank test and the chamber where the specimen is mounted, the concentration in the two chambers according to the time elapsed (initial, 30 minutes, 60 minutes, 90 minutes, 120 minutes) for each gas is measured. Measured with a gas detector tube, the ammonia gas deodorization rate results were shown according to the following [Equation 1].

[Equation 1]

Deodorization rate = (Cb-Cs)/Cb×100

Cb: concentration of the blank test chamber after a certain period of time has elapsed

Cs: the concentration of the chamber into which the sample is added after a certain period of time has elapsed

[Table 4]

As a result, in the subcritical extracts of single raw materials of plants of Test Examples 1 to 6, ammonia gas deodorization rates of 38% to 54% were measured after 60 minutes, but the six plant subcritical complex extracts of Test Example 7 were obtained after 60 minutes. After 98% ammonia gas deodorization rate was measured.

[Table 5]

In addition, the 6 types of subcritical complex extract of Test Example 7 is excellent in ammonia gas deodorization compared to the 38% deodorization rate of the 6 types of hot water complex extract of Comparative Example 1 or the 46% deodorization rate of the 6 types of distillation complex extract of Comparative Example 2 It was confirmed that it had an effect.

Through the above results, it was confirmed that the subcritical extract combination of six components of gold, cedar, cypress, cypress, bamboosundae, and fir tree exerts a synergistic effect by exerting an elevated ammonia gas deodorization effect, and subcritical extraction method It was confirmed that the ammonia gas deodorization effect was further strengthened.

Example 5. Measurement of the inhibitory effect of isovalic acid production

Regarding body odor, a substance contained in sweat, etc., and a substance that induces bad odor is isovaleric acid having a structure of the following formula (1).

[Formula 1]

In relation to the production mechanism of isovalic acid, leucine dehydrogenase enzyme that induces the conversion of α-ketoisocaproic acid from L-Leucine to inhibit the production of α-ketoisocaproic acid, a precursor of Isovaleryl CoA By inhibiting it, it is possible to block the production of isovalic acid.

From the above facts, since there is a limit to achieving the purpose of deodorization simply by suppressing perspiration, the effect of inhibiting the production of isovalic acid, which is the causative agent of bad odor, that is, the inhibitory effect of the enzyme leucine dehydrogenase was confirmed. Samples for performing the experiment were prepared according to the type, composition, and concentration of the extract used in the mixture, using the subcritical extracts of Test Examples 1 to 7 prepared in Example 1.

For a single sample among the samples, each of the plant extracts prepared in the above example was treated by concentration, and in the case of a composite sample, a composite sample in which the extracts to be mixed were mixed at the same content was used. The isovalic acid production inhibitory effect of the prepared sample was confirmed as an enzyme activity inhibitory effect on leucine dehydrogenase.

More specifically, 2.5U/mL of leucine dehydrogenase was diluted in 100mmol/L glycine buffer (pH 9.5) to prepare 10uL, and the samples of Test Examples 1 to 7 were treated at a concentration of 10.0mg/mL. After processing the sample, 0.75 mL of 250 mmol/L glycine buffer (pH 10.5), 0.5 mL of 60 mmol/L L-leucine, 100 mmol NAD ⁺ 49 uL and 171 uL of purified water were added and reacted. After the reaction, absorbance was measured at 405 nm. Using the change in the measured absorbance, the enzyme activity was calculated through the amount of NADH generated, and the results are shown in [Table 6]. Enzyme activity shown in [Table 6] was shown as a comparative activity when the untreated group was 100%, that is, a known substance known as an enzyme inhibitor, that is, an aluminum chlorohydrate compound (positive control) as a comparison group. Instead, the processing results are shown. The results in [Table 6] below are shown as average values of the results of repeating the treatment three times in total.

[Table 6]

As a result, in the test groups of Test Examples 1 to 6, which were treated with the subcritical extract of a single plant raw material of Test Examples 1 to 6, the leucine dehydrogenase enzyme activity was measured to be 72% to 95%, and the leucine dehydrogenase enzyme It was observed that it was not effective in inhibiting the production of isovalic acid, which is the cause of odor. In contrast, in the test group treated with the six plant subcritical complex extracts of Test Example 7, the leucine dehydrogenase enzyme activity was measured to be 42%, which was shown to be effective in inhibiting isovalic acid production.

In addition, it can be confirmed that the six subcritical complex extracts of Test Example 7 have very excellent leucine dehydrogenase enzyme inhibitory activity compared to the six types of hot water complex extracts of Comparative Example 1 or the six types of distillation complex extracts of Comparative Example 2. there was.

Through the above results, it was confirmed that the subcritical extract combination of six components of gold, cedar, cypress, cypress, bamboosundae, and fir tree exerts elevated leucine dehydrogenase enzyme inhibitory activity to create synergy, It was confirmed that the leucine dehydrogenase enzyme inhibitory activity was further enhanced through the subcritical extraction method.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

Golden ( Scutellaria baicalensis ) subcritical extract, cedar ( Cryptomeria Japonica ) subcritical extract, cypress ( Chamaecyparis Obtusa ) subcritical extract, Thujopsis Dolabrata subcritical extract, bamboo sundae ( Phyllostachys Heterocycla ) subcritical extract, and Abies Sibirica ) contains a complex extract composed of subcritical extracts as an active ingredient,
The subcritical extraction is characterized in that the subcritical extraction is performed at a temperature of 180 to 200 ° C and a pressure of 100 to 130 atmospheres,
The complex extract is Escherichia coli KCTC2571, Pseudomonas aeruginosa KCTC2513, Staphylococcus aureus KCTC3881, Candida albicans (Candida albicans), and Aspergillus KCTC7965 It is characterized in that it exhibits antibacterial activity against Aspergillus brasiliensis KCTC6317),
The complex extract is characterized in that ammonia gas is removed,
The complex extract is characterized in that it inhibits the production of isovalic acid by inhibiting the leucine dehydrogenase enzyme activity,
Antibacterial and deodorizing composition. delete delete delete delete According to claim 1,
The composition is an antibacterial and deodorizing composition, characterized in that it is a cosmetic composition. delete