KR20080002224A - Method of manufacturing shampoo having antibiotic activity against staphylococcus genus and shampoo composition therefrom - Google Patents

Abstract

A method for manufacturing shampoo having antimicrobial activity against Staphylococcus genus is provided to improve stability by inhibiting phase separation phenomenon by using powder, increase antimicrobial activity by increasing the content of effective ingredients, and inhibit toxicity to human. A method for manufacturing shampoo having antimicrobial activity against Staphylococcus genus comprises the steps of: (1) independently preparing the extracts of Scutellaria baicalensis Georgi and grapefruit seeds; (2) drying the extracts of Scutellaria baicalensis Georgi and grapefruit seeds and pulverizing the dried extracts; (3) mixing the powders of Scutellaria baicalensis Georgi and grapefruit seed extracts in a ratio of 1-2:1(w/w); (4) and adding 0.5-20.0 wt.% of the Scutellaria baicalensis Georgi and grapefruit seed extract powder into a shampoo composition and mixing them.

Description

Method of manufacturing shampoo having antibiotic activity against Staphylococcus genus and shampoo composition therefrom}

1 is an electron micrograph of bacteria present in the hair.

Figure 2 is a diagram confirming the growth inhibitory effect on the staphylococcus aureus of the golden extract, grapefruit seed extract, other herbal extracts.

Figure 3 is a view confirming the antimicrobial activity against Staphylococcus aureus by the concentration of golden extract.

Figure 4 is the result of comparing the antimicrobial effect against Staphylococcus aureus in the hair treated with the antimicrobial shampoo composition and the surfactant of the present invention.

5 is an electron micrograph comparing and confirming the effect of removing Staphylococcus aureus from the hair treated with the antimicrobial shampoo composition, the general shampoo, and the comparative example composition of the present invention.

The present invention relates to a method for producing an antibacterial shampoo and an antibacterial shampoo composition prepared by the method.

Staphylococcus , Streptococcus , Lactobactillus , Escherichia , etc. inhabit our bodies as normal microorganisms, but it is known to cause opportunistic infections in some cases.

Especially staphylococci The genus is a Gram-positive bacterium present in the upper respiratory tract, nose, throat, skin, etc., and is known to be easily infected with dust particles through the air because it is resistant to dryness.

In particular, S. aureus is an opportunistic strain that causes fatal diseases in children and the elderly by developing pneumonia, osteomyelitis, sepsis, toxic shock syndrome, or acute bacterial conjunctivitis. (2003, Korean Journal of Ophthalmology, vol 44, pp. 2305-2311). To reside in the human body Staphylococcus aureus (Staphylococcus aureus ) showed 34% in the nose, 21% in the hair, and 15.5% in the facial hair among the 3,039 isolates, and more than 70% were reported to be related to human hair (2000 Japanese microorganisms). Journal, vol 44, pp. 453-656). Patients who are admitted to the intensive care unit of a hospital or long-term inpatients are more likely to have opportunistic infections caused by bacteria attached to the hair.

Therefore, it is necessary to remove these bacteria and keep them clean. However, many bacteria causing opportunistic infections secrete a protein called adhesin and are strongly attached to the hair, making it difficult to remove them with ordinary shampoo or hair cleaner. 1).

There is a method for sterilization in the attached state to remove the bacteria attached to the hair does not fall into the shampoo. To this end, it is possible to think of shampoos with antibiotics in general, but due to the indiscriminate abuse of antibiotics, various resistant bacteria are produced, which is a big problem not only in Korea, but also worldwide. Has been actively conducted to search for antimicrobial active substances and to identify active bodies.

In this regard, the present inventors searched for edible natural products with high selective antimicrobial activity in order to remove Staphylococcus aureus, a hair-adhesive opportunistic bacterium, which is difficult to remove with a general shampoo without using antibiotics.

On the other hand, when manufacturing a cosmetic or shampoo composition using a natural extract, since the phase separation is caused by a solvent when a predetermined amount or more of the extract is added to the composition, the ratio of the extract in the general shampoo is used at 0.1 ~ 0.5% or less. In this case, there is a problem in that it is not possible to add a sufficient amount to have an effect by the extract, especially in the case of the cleaning composition, such as shampoo composition when the period of application is relatively short to obtain the effect by the added extract There is no problem.

To solve the above problem,

The present inventors can replace the chemical agent used as an existing antimicrobial agent, and by selecting the golden and grapefruit seeds that have a specific antimicrobial effect against opportunistic strains Staphylococcus, and also by using the dry powder of these extracts,

Provided is a method for preparing an antibacterial shampoo containing a dry powder mixture of golden extract and grapefruit seed extract, which increases the phase stability of the final antibacterial shampoo composition and increases the content of the active ingredient having antibacterial activity in the composition, and the antibacterial shampoo composition prepared therefrom. I will.

The present invention provides a method for preparing an antibacterial shampoo containing a dry powder mixture of a golden extract and a grapefruit seed extract and an antimicrobial shampoo composition prepared therefrom.

Method for producing an antibacterial shampoo of the present invention,

1) preparing a golden and grapefruit seed extract, respectively;

2) drying and powdering the golden and grapefruit seed extracts;

3) mixing the dry powder of the golden and grapefruit seed extract in a ratio of 1-2: 1 (w: w);

4) adding the dry powder mixture of the golden and grapefruit seed extract in an amount of 0.5 to 20.0% based on the total weight of the shampoo composition and mixing the mixture uniformly.

In the preparation method of the present invention, it is preferable that 0.1 ~ 10.0% by weight of the golden extract dry powder, and 0.1 ~ 10.0% by weight of the grapefruit seed extract dry powder, based on the total weight of the shampoo composition, the dry powder mixture is based on the total weight of the composition Preferably it contains 0.5-20.0%, More preferably, it contains 2.0-10.0 weight%.

In addition, in the production method of the present invention, the bacterium is preferably Staphylococcus microorganism, in particular S. aureus .

The herbal extracts used in the composition of the present invention are dried in the form of dried herbs or dried products in the form of dried or powdered powders according to a conventional drying method, and a conventional extraction method, for example, hot water extraction or extraction with an organic solvent. To extract.

Specific method for producing a herbal extract used in the composition of the present invention, after cutting the dry herbal medicine, water of about 2 to 20 times the weight of the herbal medicine, lower alcohol having a carbon number of C1 to C4 or a mixed solvent thereof, preferably Preferably, using water or ethanol or 70% ethanol, the extraction method such as hot water extraction, cold extraction, ultrasonic extraction or reflux cooling extraction for about 2 hours to 10 days at an extraction temperature of room temperature to 100 ℃ After extracting repeatedly, the extract is prepared by filtration, concentrated under reduced pressure or lyophilization or spray drying.

According to an embodiment of the present invention, the golden extract is first obtained by adding gold and distilled water at a ratio of 1:10 (V / V) and heating for 1 hour to filter the extract obtained with a Millipore filter (0.45㎛) and the filtered liquid is sprayed A dry powder is used as a golden extract to be added to the shampoo using a spray dryer.

The solvent exhibits a preferable extraction effect to use at least twice the weight of the herbal medicine, when using more than 20 times the solvent tends to take a lot compared to the extraction amount.

Grapefruit seed extract of the present invention may be prepared by the same method as described above, it may be used commercially available.

The golden extract used in the antimicrobial shampoo preparation method of the present invention exhibits antimicrobial activity against gram-positive bacteria and the grapefruit seed extract exhibits the antimicrobial activity against gram-positive and negative bacteria, in particular the golden and grapefruit used in the antimicrobial shampoo preparation method of the present invention. Seed extract mixed powder shows strong antimicrobial activity against Staphylococcus microorganisms, in particular Staphylococcus aureus.

In the fourth step of the manufacturing method of the present invention, the shampoo composition contains a thickener, a surfactant, an emulsifier, a hair conditioner, a water-soluble inorganic salt, a pH adjuster, a humectant, a preservative, a solvent, a dye, a perfume, and the like, which are conventionally contained in the shampoo. do.

The thickener is methyl cellulose, carboxymethyl cellulose, carboxymethyl hydroxyguanine, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer, cocaramide die, polyquaternium, cetearyl alcohol, stearic acid, carrageenan It may be one kind or two or more kinds selected from conventionally used thickeners.

Such surfactants include anionic surfactants, cationic surfactants, nonionic surfactants, amphoteric surfactants.

Anionic surfactants among the surfactants are specifically ammonium laurylsulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isionate, sodium lauryl isceionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium lauryl Ether sulfate (1-3 ethylene oxide) and the like.

The cationic surfactant in the surfactant may specifically be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, or the like.

Among the surfactants, the nonionic surfactant may specifically be polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, polysorbate 80, hardened castor oil derivative, fatty acid diethanolamide, glyceryl stearate, or the like. .

Among the surfactants, the amphoteric surfactant may be specifically betaine, amidebetaine type, sulfobetaine type, steartriammonium chloride, or the like.

The emulsifier is a span series or sorbitan monolaurate, sorbitan monooleate, such as span series or polyethylene oxide-5 sorbitol oleate (polyethyleoxide-5 sorbitol oleate). It may be one or more selected from glycerol monostearate, polyethylene oxide derivative tween series or Myrj series and span series.

The hair conditioner may be used alone or in combination of silicone components such as silicone or silicone gum and oil components such as ester oil and mineral oil.

As the water-soluble inorganic salt, sodium chloride, sodium nitrate, sodium bromide, potassium chloride, potassium bromide, or the like can be used.

In addition, the pH adjusting agent may be any of organic acids such as citric acid, succinic acid, tartaric acid, lactic acid, malic acid, fumaric acid, maleic acid, glycolic acid, inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, and the like. Sodium salt, potassium salt or ammonium salt can be used in combination, and triethanolamine, sodium chloride, sodium hydroxide, potassium hydroxide, etc. can also be used.

Specifically, the moisturizing agent is one of the moisturizing ingredients used in the past, such as ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, sorbitol, and collagen. Or two or more types can be mixed and used.

Specifically, the preservative may be benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMH hydantoin, methyl paraben, ethyl paraben, propyl paraben, and the like.

The solvent may be specifically purified water, ethanol, Tween 20, cyclomethicone, mineral oil, dimethicone and the like.

The fragrances and colors may be used in the components used in general hair cleaner.

In addition, in the antimicrobial shampoo manufacturing method of the present invention, the shampoo may be formulated as a liquid, solid soap, or powder.

In addition, the present invention relates to an antimicrobial shampoo prepared by the method for preparing the antimicrobial shampoo and containing 0.5 to 20.0% of a dry powder mixture of golden and grapefruit seed extracts based on the total weight of the shampoo composition.

When preparing a cosmetic or shampoo composition using a natural extract, since the phase separation is caused by a solvent when a certain amount of the extract of the composition is added, the ratio of the extract in the general shampoo is used at 0.1 to 0.5% or less. In this case, there is a problem in that it is not possible to add a sufficient amount to have an effect by the extract, especially in the case of the cleaning composition, such as shampoo composition when the period of application is relatively short to obtain the effect by the added extract There is no problem.

In addition, when the extract is added to the shampoo composition, the viscosity decreases in the final composition, so that phase separation occurs easily, so that a large amount of thickener is used, thereby increasing the cost. Therefore, there is a problem in that the appearance and efficacy of the product, or become uneconomical, so that only a small amount of a certain amount or less is added.

However, in the case of the antimicrobial shampoo manufacturing method of the present invention, the freeze-dried powder does not occur phase separation phenomenon, thereby increasing the stability of the product, and also has a high antibacterial activity against opportunistic strains of golden and grapefruit seeds. It can be made to contain the extract active ingredient in a sufficiently high dose to exhibit an effect that can effectively prepare a shampoo having excellent antimicrobial activity.

In addition, the antimicrobial shampoo composition of the present invention prepared by this production method is also excellent in human safety by containing an antibacterial component obtained from natural products.

Hereinafter, preferred examples are provided to help understanding of the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

[ Example Preparation of Shampoo Composition Containing Dry Powder Mixture of Golden and Grapefruit Seed Extracts of the Present Invention

1) Preparation of Golden Extract

In order to prepare the shampoo composition of the present invention, 0.1 kg of dried golden (root) was mixed with 1 liter of distilled water, and hot water was extracted using a shaker until the final volume became 300 ml to prepare a golden extract.

2) Preparation of Grapefruit Seed Extract

0.1 kg of the crushed grapefruit seeds were mixed with 1 liter of 100% ethanol, and then hot water extracted using a shaker to prepare a grapefruit seed extract.

3) of golden and grapefruit seed extract Powder

300 ml of the golden extract and 300 ml of grapefruit seed extract were vacuum filtered and concentrated under reduced pressure using a millipore filter (0.45 μm), respectively, and sprayed / frozen dried, respectively, 22 g and 13 g in powder form. Obtained.

4) Determination of antimicrobial activity of golden and grapefruit seed extracts

The antibacterial activity of the golden and grapefruit seed extracts was measured using the Agar hole method.

First, the antibacterial activity was used Staphylococcus aureus strains.

After incubating the bacteria in a liquid medium, the number of bacteria was measured and plated in the same amount in a plate count Agar (PCA) solid medium, and then incubated for 48 hours in an anaerobic state at 37 ℃. Then, a hole about 1 cm in diameter was made in the plate inoculated with bacteria, and the golden and grapefruit seed extracts obtained by the method of 1 and 2 were diluted in 1% in distilled water and dropped in an amount of 100 μl. After that, the diameter of the clear ring (clear zone) produced by further culturing for 24 hours (bacteria) was measured, and the antibacterial activity was measured.

Transparent ring is a part of the growth inhibition of bacteria, can determine the antimicrobial activity by the extract through the diameter size of the transparent ring.

In addition, in this case, for the comparison of antimicrobial activity, extracts of Angelica, red ginseng, allium, phosphorus, ginkgo biloba, pine needles, eoseongcho, persimmon leaves were prepared by the method of 1, 2), and antimicrobial experiments were used.

The size of the inhibitory ring (Agar hole method) for Staphylococcus aureus of the extracted drugs is shown in Figure 2 ((a); gold, (b); Angelica, (c); red ginseng, (d) (B); grapefruit seeds, (b); ginkgo biloba, (c); pine needles, (d); eochocho, (e); persimmon leaf extract).

Referring to the results of Figure 2, the test of the various herbal extracts, it was found that the antibacterial activity of the gold and grapefruit seed extract is the best.

In addition, the antibacterial activity (1%, 0.5%, 0.1%, 0.05%) by the dilution concentration of the golden extract against Staphylococcus aureus was confirmed by the Agar hole method as described above.

3, it can be seen that as the concentration of the golden extract increases, the size of the inhibitory ring increases and the antimicrobial activity is proportional.

In addition, the antibacterial activity was tested as follows to demonstrate the efficacy of various bacteria of the golden and grapefruit seed extract.

Antibacterial activity is Bacillus subtilis subtilis ), Staphylococcus aureus ), Serratia sp.), E. coli , Salmonella spp. sp.), Shigella species sp.) Aspergillus niger , A total of seven strains were used.

After incubating the bacteria in a liquid medium, the number of bacteria was measured and inoculated in a PCA solid medium in the same amount, and then incubated for 48 hours in an anaerobic state at 37 ℃. A hole about 1 cm in diameter was made in each inoculated plate, and the golden extract dried powder and grapefruit seed extract dried powder obtained by the method of 1, 2, and 3 were added to 0.05% and 0.1% in distilled water. After diluting and dropping to an amount of 100 μl, the diameter of the transparent ring formed by further culturing for 24 hours (bacteria) or 48 hours (fungus) was measured, and antimicrobial activity was measured.

The experimental results are shown in Table 1.

   Strain   Gram +/- Inhibitory ring size Golden extract Grapefruit seed extract Dilution Ratio 0.05% Dilution Ratio 0.1% Dilution Ratio 0.05% Dilution Ratio 0.1% Bacillus subtilis + 1.0 1.5 1.2 1.5 Staphylococcus aureus + 0.7 1.8 1.5 2.0 Serratia sp . - 0 0.5 0.5 1.0 E. coli - 0 0.7 1.0 1.5 Sallmonella sp. - 0 0.5 0 0.5 Shigella sp. - 0 0.5 1.0 1.5 Aspergillus niger 0.2 0.5 1.5 2.0 Unit: cm

Referring to Table 1, the golden extract showed antimicrobial activity against Gram-positive Bacillus subtilis, Staphylococcus aureus, and showed antimicrobial activity against various test strains at a concentration of 0.1%, among the test strains Staphylococcus aureus ) was the most effective.

For a grapefruit seed extract, it showed a strong antibacterial activity against Gram-positive and Gram-negative bacteria, the most effective was superior to Staphylococcus aureus (Staphylococcus aureus).

In addition, the gold and grapefruit seed extracts exhibited antifungal activity against Aspergillus niger, a fungus.

5) Determination of antimicrobial activity of golden and grapefruit seed extract dry powder mixtures

In order to compare the antimicrobial activity of the golden, grapefruit seed mixed extract and the dry powder mixture of these extracts were tested as follows.

The golden and grapefruit seed extracts of 1) and 2) were mixed at a ratio of 1: 1 (v: v) to prepare a mixed extract, and the dry powder of the golden and grapefruit seed extracts of 3) was 2: 1 (w). to a dry powder mixture.

This was dissolved in distilled water by concentration to measure the antimicrobial activity.

The results are shown in Table 2, and it was confirmed that the dry powder mixture exhibited about 30 times or more antibacterial activity than the mixed extract of golden and grapefruit seed extracts.

Comparison of Antimicrobial Activity of Mixed Extracts and Dry Powder Mixtures   Strain Inhibitory ring size Mixed Extract Dry powder mixture Dilution Ratio 0.5% Dilution Ratio 1.0% Dilution Ratio 0.05% Dilution Ratio 0.1% Staphylococcus aureus 0.6 1.1 2.0 3.2 Unit: cm

6) Preparation of Antimicrobial Shampoo Composition of the Present Invention

The antimicrobial shampoo composition and the comparative example composition of the present invention were prepared by mixing the components and contents of Table 3 below.

Optimal compounding ratio (%) of the antimicrobial shampoo composition of the present invention Raw material name Preparation Example 1 Preparation Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Process water 33.38 31.38 35.38 33.38 33.38 Thickener 0.15 0.15 0.15 0.15 0.15 Sodium chloride 2.00 2.00 2.00 2.00 2.00 Anionic surfactant 50.00 50.00 50.00 50.00 50.00 Cationic Surfactants 4.00 4.00 4.00 4.00 4.00 Nonionic surfactant 2.00 2.00 2.00 2.00 2.00 Emulsifier 1.40 1.40 1.40 1.40 1.40 Advanced Alcohol 0.80 0.80 0.80 0.80 0.80 Amphoteric surfactants 0.80 0.80 0.80 0.80 0.80 paraffin 1.00 1.00 1.00 1.00 1.00 Silicone 1.50 1.50 1.50 1.50 1.50 keratin 0.10 0.10 0.10 0.10 0.10 Panthenol 0.05 0.05 0.05 0.05 0.05 Spices 0.80 0.80 0.80 0.80 0.80 Citric acid 0.07 0.10 0.10 0.10 0.10 Sodium propionate 0.10 0.07 0.07 0.07 0.07 Golden Extract Dry Powder 1.00 2.00 - 2.00 - Grapefruit Extract Dry Powder 1.00 2.00 - - 2.00

7) Antibacterial and antibacterial shampoo composition of the present invention Antifungal  Activity measurement

The antimicrobial effect of the antimicrobial shampoo composition of the present invention was measured as follows.

Shampoo composition prepared according to the composition of Table 3 Preparation Example 1, 2 and Comparative Examples 1, 2, 3 composition was diluted in distilled water at a concentration of 10.0% to test the antibacterial and antifungal activity in the same manner as in 4) It was.

The results are shown in Table 4.

Antibacterial and Antifungal Activity of the Antibacterial Shampoo of the Present Invention  Strain Inhibitory ring size Preparation Example 1 Preparation Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Bacillus subtilis 1.1 2.0 0 0.3 0.7 Staphylococcus aureus 1.5 2.5 0 0.4 0.8 E.coli 0.7 1.0 0 0.1 0.3 Sallmonella sp. 0.5 1.0 0 0.1 0.3 Shigella sp. 1.1 1.5 0 0.2 0.4 Aspergillus niger 1.0 2.0 0 0.2 0.5 Unit: cm

8) Confirm the antimicrobial effect on the hair of the antimicrobial shampoo composition of the present invention

When the antibacterial shampoo of the present invention was treated to hair infected with Staphylococcus aureus, the antimicrobial effect was tested.

First, the same human hair surfactant and main composition of the shampoo and the composition of the antimicrobial shampoo preparation example 2) according to the concentration (5, 10, 20%) after treatment and washing, then placed in a PCA solid medium, 37 Incubation was carried out for 24 hours in an anaerobic state of ℃.

4 is a result of comparing and confirming the removal effect against Staphylococcus aureus in the hair treated with the antimicrobial shampoo composition and the surfactant of the present invention [control: control (S. aureus infected hair), (a); SDS 5% treatment, (b); SDS 10% treatment, (d); SDS 20% treatment, (d); Antibacterial shampoo 5% treatment, (e); Antibacterial shampoo 10% treatment, (f); Antibacterial Shampoo 20% Treatment]

The antimicrobial shampoo of the present invention was treated with low and high concentrations of hair (d), (e), it can be seen that the bacteria do not proliferate in all can be confirmed that the effective removal. On the other hand, hair (a), (b), (c) treated with a surfactant, which is the main component of the shampoo, tends to decrease with concentration, but it can be seen that Staphylococcus aureus proliferates.

Therefore, it was confirmed that the Staphylococcus aureus can be effectively removed by the antibacterial shampoo of the present invention.

In addition, the hair of the same person was washed with 5 ml each of the general shampoo, Comparative Example 3) composition, water, and the composition of the antimicrobial shampoo preparation example 2) of the present application, and then photographed by electron microscopy (SEM) of the hair-adhesive Staphylococcus aureus. The removal was confirmed.

5 is an electron micrograph showing the effect of removing staphylococcus aureus in the hair treated with the antibacterial shampoo of the present invention (A: general shampoo, B: Comparative Example 3 composition, C: water, D: antibacterial shampoo treatment).

Staphylococcus aureus attached to the hair was not removed in all of the experimental groups A, B, and C, and was removed in the experimental group of D. Experimental results showed that C <B <A <D in order to remove. This is confirmed that even using a normal shampoo or grapefruit seed extract-containing shampoo can not effectively remove the staphylococcus aureus attached to the infected hair, it can be effectively removed by the antibacterial shampoo of the present invention.

9) of the antibacterial shampoo of the present invention Stability  Confirm

After the accelerated test with an antibacterial shampoo containing a dry powder mixture (1: 1 (w: w)) and an extract (1: 1 (v: v)) of the golden and grapefruit seed extract of the present invention, The change in viscosity was tested.

The results are shown in Table 5.

Sample containing shampoo Viscosity (Unit: cps) Shampoo base Initial addition 1 day later 2 days later 7 days later After 20 days 30 days later Contains dry powder mixture One% 8000 7800 7800 7800 7800 7800 7800 2% 8000 7500 7200 7200 7200 7100 7100 3% 8000 7300 7200 7200 7000 7000 7000 4% 8000 7000 6900 6800 6800 6800 6600 5% 8000 6800 6700 6700 6700 6600 6400 Contains extract One% 8000 7000 6700 6200 6000 5900 5900 2% 8000 6400 6000 5500 5000 4800 4800 3% 8000 6000 5000 4800 4500 4400 4200 4% 8000 5500 4400 4000 3900 3800 3200 5% 8000 4800 4000 3900 3800 3300 3000

Looking at the results, it can be seen that in the case of the shampoo added 1 ~ 5% of the extract, the viscosity was measured to be less than 6000 cps, which is the normal shampoo viscosity after 30 days of the accelerated test, indicating that the viscosity was significantly decreased. Also, as the addition ratio of the extract increased, the viscosity dropped further, and when 5% of the extract was added, the viscosity dropped to half the level of 6000 cps, which is the normal shampoo viscosity, after 30 days, indicating that there was a problem in phase stability. have.

On the other hand, in the case of the shampoo containing the dry powder mixture of the present invention, it can be seen that after the increase of the addition ratio and 30 days, the normal shampoo viscosity of more than 6000cps is satisfied, it can be confirmed that the phase stability meets the standard value. .

As described above, the production method of the present invention uses a freeze-dried powder to increase the stability of the product because the phase separation does not occur, and also has a high antimicrobial activity of gold and grapefruit seed extract effective ingredient selective opportunistic strains It can be contained in a sufficiently high dose so that a shampoo having excellent antimicrobial activity can be effectively produced. In addition, the shampoo composition of the present invention prepared by this manufacturing method is also excellent in human safety by containing an antibacterial component obtained from natural products.

Claims (5)

Method for producing an antibacterial shampoo containing a dry powder mixture of the golden extract and grapefruit seed extract consisting of the following steps; 1) preparing a golden and grapefruit seed extract, respectively; 2) drying and powdering the golden and grapefruit seed extracts; 3) mixing the dry powder of the golden and grapefruit seed extract in a ratio of 1-2: 1 (w: w); 4) adding the dry powder mixture of the golden and grapefruit seed extract in an amount of 0.5 to 20.0% based on the total weight of the shampoo composition and uniformly mixing. The method of claim 1, wherein the dry powder mixture of the golden and grapefruit seed extracts of step 4) is added in an amount of 2.0 to 10.0% based on the total weight of the shampoo composition. The method of claim 1, wherein the bacterium is a microorganism of glucose cocci. The method of claim 3, wherein the microorganism of the genus Glucose is Staphylococcus aureus. An antibacterial shampoo prepared by the method of any one of claims 1 to 4 and containing 0.5 to 20.0% of a dry powder mixture of golden and grapefruit seed extracts based on the total weight of the shampoo composition.

Images