JP2019031455A - Antibacterial agent, deodorant, and cosmetic for deodorant, containing cypress acid - Google Patents

Abstract

To provide an antibacterial agent, a deodorant and a cosmetic for deodorant, excellent in antibacterial property.SOLUTION: The antibacterial agent, the deodorant and the cosmetic for deodorant contain effective dose of cypress acid.SELECTED DRAWING: None

Description

  The present invention relates to an antibacterial agent, a deodorant, and a deodorizing cosmetic material that contain hinokic acid and have excellent antibacterial properties, particularly antibacterial properties against bacteria that cause odors.

  Hinokic acid is a natural compound found in cypress leaves. It has already been reported that hinokic acid has an antifungal action against Trichophyton mentagrophytes (Non-patent Documents 1 and 2). However, it has been reported that only 0.08% of hinokic acid is purified from the essential oil obtained by the usual distillation method (Non-patent Document 3).

Holzforschung, vol. 48 (1994), pp 193-198. “Forest Biomass Utilization Society Journal” Vol. 8 (2013), pp 13-19 “Pharmaceutical Journal” Vol. 72 (1952) pp703-705

  As described above, only 0.08% of hinokic acid is purified from an essential oil obtained by a normal distillation method, and it has been difficult to obtain a hinokic acid in an amount sufficient for practical use. For this reason, compared with hinokitiol, research on various properties of hinokic acid and industrial utilization of hinokic acid are not progressing.

  An object of the present invention is to provide an antibacterial agent, a deodorizing agent, and a deodorizing cosmetic material that contain hinocic acid and have excellent antibacterial properties, in particular, antibacterial properties against bacteria that cause odors.

  The present inventors succeeded in extracting and purifying hinokic acid from a cypress plant material containing hinokic acid more efficiently than before. As a result of diligent research on the properties of the obtained hinokic acid, the present inventors have newly discovered that hinokic acid is excellent in antibacterial properties, particularly in antibacterial properties against bacteria that cause odors.

  The antibacterial agent, deodorant, and deodorant cosmetic (hereinafter collectively referred to as “antibacterial agent etc.”) of the present invention contain an effective amount of hinokic acid.

  The antibacterial agent, deodorant, and deodorant cosmetic composition of the present invention are excellent in antibacterial properties, particularly antibacterial properties against bacteria that cause odors, and therefore can be used for antibacterial or deodorant applications. it can.

It is a schematic diagram of the high pressure steam distillation apparatus in an Example.

  Embodiments of the present invention will be described below. This embodiment is an example for carrying out the present invention, and the present invention is not limited to this embodiment.

  The hinokic acid may be isolated, purified or synthesized hinokic acid, or may be an extract containing hinokic acid, for example, an extract (such as an essential oil) obtained by extraction from a cypress family raw material. The specific content of the cypress plant material is not limited as long as it is a part of a cypress plant containing hinokic acid. Specific examples of the cypress family include Taiwan cypress, Western red cedar, Asunaro, Taiwan hiba, Taiwan cypress, and incense cedar. The cypress plant material may be used alone or in combination with two or more cypress plant materials. Further, as the part, a leaf and a branch and leaf part including a leaf are preferably used. Non-Patent Document 3 reports that hinokitiol is not detected in the acidic part of hinoki leaf oil and the central component is hinokic acid. Therefore, by using an extract from a leaf and a branch and leaf part including the leaf, effective utilization of the leaf and the branch part including the leaf that has not been used in relation to hinokitiol can be expected.

  There is no particular limitation on the method for obtaining the extract containing hinocic acid. The extract can be obtained, for example, by a high-pressure steam press distillation method using an apparatus described in Japanese Patent No. 4388715.

  The “antibacterial” (antibacterial effect) means a bacteriostatic action and / or a bactericidal action. That is, it may have both a bacteriostatic action and a bactericidal action, or may have only one of them. The bacteriostatic action means inhibiting or preventing the growth of bacteria, and the bactericidal action means killing or removing part or all of the bacteria.

  The “effective amount” is not particularly limited as long as it exhibits an antibacterial effect. The effective amount can be, for example, 100 mg / L (ppm) or more, or 200 mg / L (ppm) or more in the total amount. The upper limit value of the effective amount is not particularly limited as long as it exhibits an antibacterial effect, and can be appropriately set in consideration of usage, cost, and the like. The upper limit value of the effective amount can be, for example, 500 mg / L (ppm) (in addition, the description of the upper limit value of the effective amount recognizes that the antibacterial effect is not achieved at a content exceeding the upper limit value. Not described.)

  The antibacterial agent or the like of the present invention may contain other components as necessary as long as the antibacterial property is not inhibited. Examples of the other components include various known additives in cosmetics and pharmaceuticals. Examples of additives used in the cosmetic field include oily components, surfactants, lower alcohols, humectants, pigments, ultraviolet absorbers, antioxidants, and fragrances. Further, as the other components, other active components such as other antibacterial agents, vitamins, protein hydrolysates, anti-inflammatory agents, and whitening agents can be used. The other components may be used alone or in combination of two or more.

  There is no limitation in particular in the kind of bacteria used as the antibacterial agent of this invention. The antibacterial agents and the like of the present invention are particularly known as bacteria that cause unpleasant odors in the body, for example, the genus Staphylococcus known as causative bacteria of aging odor and causative bacteria of odor Excellent antibacterial effect against bacteria belonging to the genus Corynebacterium. Examples of the bacterium belonging to the genus Staphylococcus include Staphylococcus aureus and Staphylococcus epidemidis. Examples of the bacterium belonging to the genus Corynebacterium include Corynebacterium xerosis.

  There is no limitation in particular in the use of the antibacterial agent of this invention. The antibacterial agent of the present invention may be used as it is for applications requiring antibacterial properties, or may be used as an additive added to products requiring antibacterial properties. Specific embodiments of the antibacterial agent of the present invention include, for example, medical agents (including drugs), cleaning agents, cosmetics, foods and drinks, medical devices, sanitary materials, and functional materials. As a further specific embodiment of the antibacterial agent of the present invention, an embodiment in which the antibacterial agent of the present invention is used directly on the human or animal body such as cosmetics or foods and drinks can be mentioned. As a further specific embodiment of the antibacterial agent of the present invention, there is an embodiment used for various products in order to impart antibacterial properties. As a further specific embodiment of the antibacterial agent of the present invention, there can be mentioned an embodiment in which various products (for example, resin film, fiber product) imparted with antibacterial properties are obtained by adding to the resin or fiber.

  There is no limitation in particular in the use of the deodorizer of this invention. The deodorant of the present invention may be used as it is for applications that require deodorizing properties, and can be used as an additive added to products that require deodorizing properties. As a specific embodiment of the deodorant of the present invention, for example, an embodiment in which the deodorizer is used directly on a human or animal body such as cosmetics or foods and drinks can be mentioned. As a further specific embodiment of the antibacterial agent of the present invention, there is an embodiment used for various products in order to impart deodorant properties. For example, the deodorizer of the present invention can be used for deodorizing textiles such as clothes, bags, bedding, curtains, sofas, cushions, carpets, and stuffed animals. In addition, the deodorant of the present invention can be added to the cleaning agent in order to impart deodorizing properties to clothing and the like. As a further specific embodiment of the deodorant of the present invention, there is an embodiment in which various products (for example, resin film, fiber product) imparted with deodorizing properties are added by adding to the resin or fiber. As a further specific embodiment of the deodorant of the present invention, there is an indoor deodorization aimed at deodorization in a certain closed space. Specific examples of the closed space include a room, a closet, a toilet, a shoe box, and a car interior. Specific embodiments of the indoor deodorization include, for example, an arrangement in a space, hanging, spraying, or application, an addition to a humidifying liquid used in an air cleaner or a humidifier, an air cleaner, and ventilation. The aspect applied to the filter used for an apparatus and an air conditioner is mentioned.

  The deodorant cosmetic composition of the present invention is not particularly limited to specific embodiments as long as it can be applied to a human or animal body for the purpose of deodorization. For example, there is no limitation in particular in the application location of the deodorant cosmetics of this invention. Examples of the application location include skin (whole body or part) and hair. Specific embodiments of the deodorant cosmetic of the present invention include, for example, cosmetics, hair care products, toiletry products (soaps, hand soaps, body soaps, facial cleansers, toothpastes, etc.), and deodorant products (antiperspirants, Deodorant, fragrance and the like.

  There are no particular limitations on the dosage form and usage of the antibacterial agent of the present invention. Specific examples of the dosage form of the antibacterial agent of the present invention include aqueous solutions, emulsions, gels, ointments / waxes, foams, solids / powder, and mists. The antibacterial agent or the like of the present invention can be used by directly attaching to the surface or inside of a human or animal body or various products, or can be used by spraying it on an indoor environment.

  Hereinafter, the present invention will be described specifically by way of examples. In addition, this invention is not limited to the form shown in the Example. The embodiment of the present invention can be variously modified within the scope of the present invention depending on the purpose and application. Further, it is added that all the considerations on the results in the examples are only the views of the inventor and do not explain the purpose of defining the present invention.

(1) Preparation of hinokic acid High-pressure steam distillation is performed using a wood compression molding apparatus (Type HVI-40 / 58, Hisaka Works Ltd., Osaka) equipped with a steam recovery valve, a cooling device, and a precipitation tank. It was. A schematic diagram of the high-pressure steam distillation apparatus of the example is shown in FIG.

  The hinoki cypress essential oil is filled with the cypress branches and leaves (500 g) immediately after collection into the distillation / compression jig (cylindrical shape with a diameter of 200 mm) of the apparatus, and distilled for 2 hours while keeping the steam in the steam kettle at 180 ° C. Got. As a control, hinoki essential oil was obtained by performing atmospheric distillation (100 ° C.) for 10 hours using “PureStiller Standard / M” (Type SHJK25L, Kohga International Trading Co., Ltd., Fukuoka).

  10 mL of 0.1N sodium hydroxide solution was added to 10 mL (271.7 mg equivalent) of the obtained cypress essential oil and stirred for 1 hour. After standing overnight, the aqueous layer was collected. The same operation was repeated two more times, and the collected aqueous layers were combined into one. To the collected aqueous layer (30 mL), 10 mL of ethyl acetate was added and stirred for 1 hour. After allowing to stand until the aqueous layer was separated, the aqueous layer was recovered. The recovered aqueous layer was allowed to stand until crystals precipitated, and then heated at 85 ° C. or higher for 1 hour. After slow cooling, the crystals were collected by filtration with a membrane filter. The recovered crystals were washed with 20% ethanol and dried to obtain hinokic acid crystals (needle crystals) (yield; 171.7 mg).

(2) Antibacterial activity test Antibacterial activity was evaluated using hinokic acid obtained by the above method. As target substances, benzalkonium chloride (manufactured by Wako Pure Chemical Industries, Ltd.), which is widely used as a disinfectant, and isopropylmethylphenol (manufactured by Wako Pure Chemical Industries, Ltd.), which is used as a preservative for cosmetics, were used. As a test cell, S. epidemidis JCM2414 strain and C.I. xerosis JCM1971 strain was used (both were obtained from RIKEN BioResource Center).

  Hinocic acid, benzalkonium chloride, and isopropylmethylphenol were each dissolved in DMSO (4 mg / mL). Test solutions prepared to various concentrations (final concentrations; 400, 200, 100, 50, 25, 12.5 mg / L) were prepared by appropriately diluting the solution. The cell stock (glycerol) was added to a medium for measuring MIC (manufactured by Nissui Pharmaceutical) and cultured for 24 hours. The culture solution was diluted 1/20 (OD600 = 0.1) with the MIC measurement medium. The diluted solution was dispensed in a volume of 135 μL into a 96-well plate. Subsequently, 15 μL of the above test solution was added and cultured. S. epidemidis is cultured at 37 ° C. for 30 hours. In xerosis, the cells were cultured at 30 ° C. for 5 days. The minimum growth inhibitory concentration (MIC; mg / L) was determined by confirming bacterial precipitation on the bottom of the well. The results are shown in Table 1.

  C. xerosis is known as a odor-causing bacterium. S. epidemidis is a causative bacterium of age-related odor. It belongs to the same genus as aureus. From Table 1, all of hinokic acid showed antibacterial activity against these strains. In addition, its antibacterial activity was superior to known isopropylmethylphenol. From these results, hinokic acid exhibits excellent antibacterial activity against bacteria that cause unpleasant odors, and can be suitably used for deodorization.

Claims (3)

  An antibacterial agent containing an effective amount of hinokic acid.   A deodorant containing an effective amount of hinokic acid. A deodorizing cosmetic containing an effective amount of hinokic acid.