KR102337919B1 - Cosmetic composition containing yellow chlorella extract - Google Patents

Abstract

It relates to a cosmetic composition comprising a yellow chlorella extract and saccharides. According to a cosmetic composition comprising a yellow chlorella extract and saccharides according to an aspect, the expression of hyaluronic acid synthase (HAS3) and FLG gene, which is a keratinocyte differentiation gene, is increased in skin cells, thereby moisturizing the skin and improving the skin barrier. appears, and significantly reduces the expression of the inflammatory cytokine IL-1β, thereby having an effect that can be usefully used for the prevention, improvement or treatment of skin damage, skin condition, or inflammatory disease.

Description

Cosmetic composition containing yellow chlorella extract

It relates to a cosmetic composition comprising a yellow chlorella extract and saccharides.

Inflammation is a physiological reaction that protects the living body from the intrusion of foreign substances such as bacteria and mechanical damage from the harmful surrounding environment, that is, excessive inflammatory reaction is also a major cause of skin damage. When inflammation occurs, various types of polynuclear leukocytes (PMNs) and immune substances increase, and these cells secrete various types of proteolytic enzymes and cytokines, which are inflammatory cell products. After that, their action may cause harmful damage to adjacent tissue cells and non-tissue cell components, and may lead to serious tissue damage of chronic inflammation. In addition, damage to the connective tissue reduces the elasticity of the skin and causes wrinkles, and furthermore, it adversely affects the regeneration and proliferation of cells, resulting in rapid skin damage.

Functional cosmetics may include products that help improve skin wrinkles by delaying skin aging, products that protect skin from UV rays and external environments, or products that help skin whitening. For the development of such functional cosmetics, the development of functional raw materials that are physiologically effective in the skin and at the same time safe should be preceded.

Chlorella (Chlorella) are two growth properties of a type of freshwater algae belonging to Chlorophyta chlorellaceae, commonly seen in melted fresh water, wetlands and dependent for growth and by photosynthesis using the growth, proliferation independent nutritional and an organic carbon source for nutrient It is a single-celled plant that contains a large amount of chlorophyll (chlorophyll a, b). The chlorella has been reported to have many effects, and through a series of studies, the chlorella extract is used for microbial or viral infection (Thnaka et al., 1986; Hasegawa et al., 1990) and for the production of various cancers (Morimoto et al., 1995; Konishi et al., 1985), but it is mainly for green chlorella extract, and specific skin protection effect or skin anti-inflammatory effect for yellow chlorella extract is not known.

One aspect is to provide a cosmetic composition comprising a yellow chlorella extract and at least one saccharide selected from the group consisting of glucose, oligosaccharide, and fructose.

Another aspect is to provide a composition for external application for skin for preventing, improving, or treating skin conditions, which includes a yellow chlorella extract and one or more saccharides selected from the group consisting of glucose, oligosaccharide, and fructose.

Another aspect is to provide a pharmaceutical composition for preventing or treating an inflammatory disease comprising a yellow chlorella extract and one or more saccharides selected from the group consisting of glucose, oligosaccharide, and fructose.

Another aspect is to provide a health functional food for improving skin condition comprising yellow chlorella extract and one or more saccharides selected from the group consisting of glucose, oligosaccharide, and fructose.

One aspect provides a cosmetic composition comprising a yellow chlorella extract and at least one saccharide selected from the group consisting of glucose, oligosaccharide, and fructose.

The cosmetic composition according to an aspect contains a yellow chlorella extract (Example 1), which has been confirmed to have a superior moisturizing effect on the skin at the same dose than the conventionally used green chlorella composition, in the cosmetic composition, and includes glucose, oligosaccharide, and fructose. By further including one or more saccharides selected from It was specifically confirmed that -1β expression was significantly reduced to exhibit an anti-inflammatory effect in skin cells (Experimental Examples 1 to 4).

The yellow chlorella extract is, for example, 1 to 30% by weight, 5 to 30% by weight, 5 to 45% by weight, 5 to 50% by weight, 7 to 35% by weight, 7 to 25% by weight based on the total weight of the composition, Or 10 to 20% by weight may be included.

The sugar may be included in, for example, 35 to 80% by weight, 40 to 75% by weight, 40 to 70% by weight, 40 to 65% by weight, 45 to 60% by weight, or 50 to 60% by weight based on the total weight of the composition. can The sugar may be included in an amount of 40% by weight or more based on the total weight of the composition in terms of moisturizing, and may be included in an amount of 70% by weight or less based on the total weight of the composition in terms of stability of the composition.

The saccharides contained in the composition include glucose, oligosaccharide and fructose, for example, in a weight ratio of 1: 0.1 to 3: 0.1 to 3, 0.5 to 2: 0.5 to 2, or 1: 0.5 to 1.5: 0.5 to 1.5 by weight, Alternatively, it may be included in a weight ratio of 1: 0.5 to 1: 0.5 to 1.

In addition, the composition may include, for example, 5 to 30% by weight of yellow chlorella extract, or 10 to 25% by weight, or 10 to 20% by weight, and 15 to 30% by weight of glucose, 15 to 25% by weight, for example. It may contain in weight % or 20 to 25% by weight, and may include, for example, 10 to 30% by weight of oligosaccharide, 10 to 25% by weight, or 10 to 20% by weight of oligosaccharide, and 5 to 20% by weight of fructose. 15% by weight, 5 to 10% by weight or 10 to 15% by weight may be included.

The composition may be for skin moisturizing, skin barrier strengthening, skin damage prevention or improvement, or skin inflammation prevention or improvement, wherein the composition increases the expression of HAS3, FLA, Aquaporin3, and Claudin1 genes in skin cells, It can inhibit the expression of interleukin-1β.

The composition may be, for example, in the form of a lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, nourishing cream, and ampoule.

The cosmetic composition may further include functional additives and components included in general cosmetic compositions in addition to the active ingredients disclosed herein, and conventionally used antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, etc. It may further include adjuvants and carriers. For example, the cosmetic composition may further include auxiliary components such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, squalane, sodium citrate, allantoin, and the like, and the solvent includes It may include hexanediol, purified water, and the like. In addition, the functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract. Other ingredients included include oils and fats, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, disinfectants, antioxidants, plant extracts, alcohols, pigments, fragrances, blood circulation promoters, cooling agents, limiting agents (制汗) agent, purified water, etc. are mentioned.

A person skilled in the art can select any additional ingredients and/or amounts thereof such that the advantageous properties of the composition according to the present disclosure are not adversely or substantially not adversely affected by the expected addition. The blending amount of the additional component can be easily selected by a person skilled in the art within a range that does not impair the purpose and effect of the present invention, and the blending amount is from about 0.001 to about 30% by weight, specifically from about 0.01 to about, based on the total weight of the composition. 25% by weight.

In addition, in the cosmetic composition of each formulation, appropriate ingredients may be selected and formulated according to the formulation or purpose of use of the cosmetic. Since the compounding component and method may follow conventional techniques, a detailed description thereof will be omitted herein.

Another aspect provides a composition for external application for skin for preventing, improving or treating a skin condition comprising a yellow chlorella extract and at least one saccharide selected from the group consisting of glucose, oligosaccharide, and fructose.

The skin condition may be any one or more selected from the group consisting of skin wounds, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, weak rash, and acne.

The term "treatment or amelioration" refers to or includes alleviation, progression inhibition or prevention of a disease, disorder, condition or condition, or one or more symptoms thereof, and "active ingredient" or "pharmaceutically effective amount" refers to a disease, disorder, may mean any amount of a composition utilized in the practice of the invention provided herein sufficient to alleviate, inhibit the progression or prevent the condition or condition, or one or more symptoms thereof. The effective amount (or dosage) may be 0.0001 mg to 10,000 mg, 0.001 mg to 1000 mg, 1.0 mg to 100 mg, 0.01 mg to 1000 mg, 0.01 mg to 100 mg, 0.01 mg to 10 mg, or 0.01 mg to 1 mg.

Examples of the skin condition or skin-related condition include skin aging, skin photoaging, wounds, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, weak rash, or acne, papule scaly disease , insect and parasite-borne diseases, superficial dermatophytosis, bacterial infectious diseases, viral diseases, adult diseases, autoimmune bullous diseases, connective tissue diseases, dyspigmentation, xeroderma pigmentosa, and the like.

The skin damage is external physical damage, penetration of chemicals, bacteria, fungi, viruses, etc., exposure to UV rays, moisture loss of the skin, wrinkles due to aging, oxidation by free radicals (active oxygen), etc., skin pigmentation, from a clinical and cosmetic standpoint, including skin inflammation, seborrheic dermatitis, redness (redness, erythema), edema, lichenification, eczema, pruritus (itchiness), and atopic dermatitis, and the like. .

The skin includes all skin parts of the body including the face, hands, arms, legs, feet, chest, belly, back, buttocks, and scalp.

In the present specification, the external preparation for skin may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermally delivered patch, drug-containing bandage, lotion, or a combination thereof. The external preparation for skin may be prepared and used in the form of an ointment, lotion, spray, patch, cream, powder, suspension, gel or gel. The external preparation for skin is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, an aqueous component, an oily component, a powder component, alcohol, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, a preservative, an antioxidant, a surfactant, a fragrance , colorant, various skin nutrients, or a combination thereof may be appropriately formulated as needed. The external preparation for skin includes metal-blocking agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belapamil, licorice extract, glablidine, and kaline. Fruit hot water extracts, various herbal medicines, tocopherol acetate, glitylittic acid, tranexamic acid and derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can be mix|blended suitably.

In addition, the composition may be a quasi-drug composition.

The term "quasi-drug" refers to textiles, rubber products, or the like, which are used for the purpose of treating, alleviating, treating or preventing diseases of humans or animals, have weak action on the human body or do not directly act on the human body, and are not devices or machines. Articles corresponding to those used for sterilization, insecticidal and similar purposes for the prevention of infection and used for the purpose of diagnosing, treating, alleviating, treating or preventing a human or animal condition or disease It refers to items that are not heavy instruments, machines, or devices, and items used for the purpose of pharmacologically affecting the structure and function of humans or animals, excluding those that are not instruments, machines, or devices. may include

Another aspect provides a pharmaceutical composition for preventing or treating an inflammatory disease comprising a yellow chlorella extract and one or more saccharides selected from the group consisting of glucose, oligosaccharide, and fructose.

Examples of the inflammatory disease or condition related to inflammation include dermatitis, allergy, atopic dermatitis, conjunctivitis, periodontitis, rhinitis, otitis media, pharyngitis, tonsillitis, pneumonia, gastric ulcer, gastritis, Crohn's disease, colitis, gout, ankylosing spondylitis, rheumatic fever, lupus, May include fibromyalgia, psoriatic arthritis, osteoarthritis, rheumatoid arthritis, periarthritis, tendinitis, tendinitis, peritenitis, myositis, hepatitis, cystitis, nephritis, sjogren's syndrome, multiple sclerosis, and acute and chronic inflammatory diseases. have.

The pharmaceutical composition may further include a pharmaceutically acceptable diluent or carrier. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and the lubricant may be magnesium stearate, talc, or a combination thereof. The carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be carboxymethyl cellulose calcium, sodium starch glycolate, anhydrous calcium monohydrogen phosphate, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

The pharmaceutical composition may be formulated as an oral or parenteral dosage form. Oral dosage forms may be granules, powders, solutions, tablets, capsules, dry syrups, or a combination thereof. The parenteral dosage form may be an injection.

Another aspect provides a health functional food for improving skin condition comprising a yellow chlorella extract and at least one saccharide selected from the group consisting of glucose, oligosaccharide, and fructose.

The health functional food composition may be used alone or in combination with other foods or food ingredients selected from the group consisting of yellow chlorella extract and glucose, oligosaccharide, and fructose, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prophylactic, health or therapeutic treatment). In general, in the production of food or beverage, the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the type of the health functional food. Among the types of health functional food, the beverage composition may contain various flavoring agents or natural carbohydrates as an additional component like a conventional beverage. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumartin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The health food composition can also be added to nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonated beverages carbonation agent used, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverage, fruit flesh for the production of vegetable beverage, or a combination thereof.

Another aspect also provides a method of preventing, ameliorating, or treating a condition in a subject comprising treating or administering to the subject in need thereof an effective amount of the composition described above.

The condition or composition of the subject is the same as described above. The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat.

According to a cosmetic composition comprising a yellow chlorella extract and saccharides according to an aspect, the expression of hyaluronic acid synthase (HAS3) and FLG gene, which is a keratinocyte differentiation gene, is increased in skin cells, thereby moisturizing the skin and improving the skin barrier. appears, and significantly reduces the expression of the inflammatory cytokine IL-1β, thereby having an effect that can be usefully used for the prevention, improvement or treatment of skin damage, skin condition, or inflammatory disease.

1 is a diagram confirming the gene expression of hyaluronic acid synthase (HAS3), water channel (Aquaporin3), and intercellular linkage factor (Claudin1) through qRT-PCR 24 hours after contact with green or yellow chlorella extract (*p < 0.05, **p < 0.01 vs. negative control).
2 is a diagram illustrating the expression of HAS3, a hyaluronic acid synthesis gene, through qRT-PCR after 24 hours of contacting a composition containing a yellow chlorella extract with the human keratinocyte line HaCaT (**p < 0.01 vs. negative control).
3 is a diagram illustrating the gene expression of filaggrin through qRT-PCR after 24 hours of contacting a composition containing a yellow chlorella extract with the human keratinocyte line HaCaT (**p < 0.01 vs. negative control).
4 is a diagram confirming the gene expression of IL-1β through qRT-PCR after 4 hours of contacting a composition containing a yellow chlorella extract with the inflammation-induced human keratinocyte line HaCaT (**p < 0.01 vs. negative control, ##p < 0.01 vs. positive control).
FIG. 5 is a diagram showing the results of photographing the skin moisture content after applying M2 to M2 including the yellow chlorella extract to the skin ( FIG. 5A ) and graphing the skin moisture content after skin application ( FIG. 5B ).

Hereinafter, examples and experimental examples will be described in detail to explain the present invention in detail. However, the Examples and Experimental Examples according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the Examples and Experimental Examples described below. The Examples and Experimental Examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1. Confirmation of moisturizing effect of yellow chlorella extract

An experiment was conducted to determine whether yellow chlorella had superior skin moisturizing effect compared to green chlorella used in a number of conventional cosmetic compositions. The green chlorella and yellow chlorella extracts used in the experiment were purchased from Daesang Co., Ltd., and 100 ppm of each of the chlorella extracts was contacted with the human keratinocyte line HaCaT, and hyaluronic acid involved in intracellular moisturizing in the cell line 24 hours after contact was synthesized. Enzyme (HAS3), water channel (Aquaporin3), and cell-to-cell link factor (Claudin1) gene expression was increased by performing qRT-PCR to confirm the increase in gene expression, which is shown in FIG. The group untreated with the extract in the cell line was used as a negative control group.

As shown in FIG. 1 , when the green chlorella extract was treated compared to the negative control, the expression of the skin moisturizing genes increased by about 1.2 times on average, and it was confirmed that the expression of the skin moisturizing genes was slightly increased. On the other hand, when the yellow chlorella extract was treated, it was confirmed that the moisturizing gene was significantly increased by about 2 times on average. Therefore, compared to green chlorella, it was confirmed that the yellow chlorella extract increased the expression of all genes involved in skin moisturizing, so that it could exhibit an excellent moisturizing effect.

Example 2. Preparation of a composition comprising yellow chlorella extract

In order to find a composition showing particularly excellent moisturizing and anti-inflammatory effects on the skin among the cosmetic compositions containing the yellow chlorella extract, which was confirmed to have an excellent moisturizing effect in Example 1, a yellow chlorella extract and a sugar containing glucose, oligosaccharide and fructose were added in the composition, respectively. They were added in different proportions, and a composition was prepared. The content of each composition is shown in Table 1 below.

Ingredient name content (wt%) M1 M2 M3 M4 Purified water 60 40 40 20 glucose
(hydrous crystalline glucose) 10 10 10 20 Oligosaccharide (oligo c-72) 10 20 10 20 fruit sugar
(HFSS 55) 10 20 20 20 Yellow Chlorella Extract
(AlgaRich Vegi-P55) 10 10 20 20

Experimental Example 1. Confirmation of the moisture improvement effect of the composition containing the yellow chlorella extract

When the yellow chlorella extract was treated on the skin, an experiment was performed to confirm whether the moisture improvement ability was increased by increasing the hyaluronic acid synthesis ability. The human keratinocyte cell line HaCaT was contacted with 0.001%, approximately 8 ppm, respectively, of the composition prepared in Example 2, and qRT-PCR was performed on the hyaluronic acid synthesis gene HAS3 24 hours after contact to confirm the increase in HAS3 gene expression, 2 is shown. A group untreated with the extract to the cell line was used as a negative control group, and a group contacted with 10 ppm of hyaluronic acid (HA) was used as a positive control group.

As shown in FIG. 2 , a significant increase in expression of the hyaluronic acid synthesis gene HAS3 was confirmed in M2 to M4 compared to the negative control group. In particular, M3 was confirmed to have an expression increase of about 2-fold compared to the positive control group, and M2 and M4 were confirmed to have an average expression increase of about 3-fold. Therefore, it was confirmed that, when the yellow chlorella extract and the sugar mixture containing glucose, oligosaccharide and fructose in a specific ratio were treated, the expression of HAS3, a gene involved in moisturizing the skin, was significantly increased, so that an excellent moisturizing effect could be obtained.

Experimental Example 2. Confirmation of skin barrier improvement effect of a composition containing yellow chlorella extract

When the yellow chlorella extract was treated on the skin, an experiment was performed to determine whether the skin barrier improvement ability was increased by inducing skin differentiation. The human keratinocyte cell line HaCaT was contacted with each of 0.001%, approximately 8 ppm of the composition prepared in Example 2, and qRT-PCR for the filaggrin (FLG) gene 24 hours after contact was performed to confirm the increase in gene expression, , which is shown in FIG. 3 . The group untreated with the extract in the cell line was used as a negative control group.

As shown in FIG. 3 , a significant increase in the expression of FLG, a keratinocyte differentiation gene, was confirmed in M2 to M4 compared to the negative control group. In particular, M2 was confirmed to have an expression increase of about 6 times compared to the negative control group, and M3 was confirmed to have an average expression increase of about 10 times. Therefore, it was confirmed that, when the yellow chlorella extract and the sugar mixture in which glucose, oligosaccharide and fructose were mixed in a specific ratio were treated, the expression of FLG, a keratinocyte differentiation gene, was significantly increased, so that an excellent skin barrier improvement effect could appear.

Experimental Example 3. Confirmation of skin inflammation improvement effect of yellow chlorella extract-containing composition

When the yellow chlorella extract was treated on the skin, an experiment was performed to determine whether the skin inflammation was improved by inhibiting the expression of interleukin-1β (IL-1β), which induces skin inflammation. Inflammation was induced by treating the human keratinocyte line HaCaT with 10 μg/ml of polyinosinic acid polycytidylic acid (Poly I:C) and 10 ng/ml of interleukin-4 (IL-4). Thereafter, each of the compositions prepared in Example 2 was brought into contact with 0.001%, approximately 8 ppm, and qRT-PCR was performed on interleukin-1β 4 hours after the contact to confirm the increase in gene expression, which is shown in FIG. 4 . The group untreated with the extract in the cell line was used as a negative control group, and the group treated with 1 μM dexamethasone showing anti-inflammatory effect was used as a positive control group.

As shown in FIG. 4 , it was confirmed that the expression of IL-1β was increased by about 5 times in the group inducing inflammation compared to the negative control group. Accordingly, it was confirmed that the group treated with the M2 composition exhibited an anti-inflammatory effect similar to that of the group treated with dexamethasone, which is a positive control group showing an anti-inflammatory effect. In particular, when the M3 composition was treated, it was confirmed that an inflammatory improvement effect of up to 60% or more appeared, and it was confirmed that it exhibits a more significant inflammatory improvement effect than dexamethasone. Therefore, it was confirmed that when the yellow chlorella extract and the sugar mixture in which glucose, oligosaccharide, and fructose were mixed in a specific ratio were treated, the expression of IL-1β in skin cells was significantly suppressed to exhibit excellent anti-inflammatory effect.

Experimental Example 4. Composition of yellow chlorella extract in vivo confirmation of skin moisturizing improvement effect in

When the yellow chlorella extract is treated on the skin, a clinical experiment was performed to confirm that moisture is actually induced by maintaining the amount of moisture in the skin. For 5 adults with an average age of 25.6 years, 100 μl of M2 to M2, which showed an excellent moisturizing effect in Practical Example 2, was applied to the arm, and after 8 hours, using Biox epsilon (Biox Systems Ltd, England), The result of photographing the amount of moisture in the skin is shown in FIG. 5A, and the result of graphing the amount of moisture in the skin after application is shown in FIG. 5B. A group treated with 500 ppm of hyaluronic acid was used as a positive control group, and a group untreated with the composition was used as a negative control group.

As confirmed in FIGS. 5A and 5B, when the amount of skin moisture is high, it is displayed brightly, and when the M2 to M4 compositions are treated, it can be confirmed that the moisture level of the skin is significantly increased compared to the negative control group. In particular, when M2 and M3 were applied, it was confirmed that the amount of moisture contained in the skin after application was large, and also when M4 was applied, it was confirmed that the skin moisture level similar to that of the positive control group appeared. Therefore, when the yellow chlorella extract and the sugar mixture in which glucose, oligosaccharide and fructose were mixed in a specific ratio were treated, it was confirmed that the skin moisturizing effect was excellent even when actually applied to the skin.

Claims (13)

Yellow Chlorella (chlorella) extract; and
A cosmetic composition comprising; sugars including glucose, oligosaccharides, and fructose,
A cosmetic composition comprising 5 to 30% by weight of yellow chlorella extract, 10 to 30% by weight of glucose, 10 to 30% by weight of oligosaccharides, and 15 to 20% by weight of fructose, based on the total weight of the composition. delete The cosmetic composition of claim 1, wherein the saccharide is included in an amount of 40 to 70% by weight. The cosmetic composition according to claim 1, wherein the saccharide comprises glucose, oligosaccharide, and fructose in a weight ratio of 1: 0.5 to 2: 0.5 to 2. delete The cosmetic composition of claim 1, wherein the cosmetic composition is for moisturizing the skin, strengthening the skin barrier, preventing or improving skin damage, or preventing or improving skin inflammation. The cosmetic composition of claim 1, wherein the composition increases the expression of HAS3, FLA, Aquaporin3, and Claudin1 genes in skin cells and inhibits the expression of interleukin-1β. The cosmetic composition of claim 1, wherein the cosmetic composition is in the form of any one of lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, nourishing cream, and ampoule. Yellow Chlorella (chlorella) extract; and
A composition for topical application to the skin for preventing, improving or treating skin conditions, including saccharides including glucose, oligosaccharides, and fructose,
The composition for external application for skin that contains 5 to 30% by weight of yellow chlorella extract, 10 to 30% by weight of glucose, 10 to 30% by weight of oligosaccharide, and 15 to 20% by weight of fructose, based on the total weight of the composition. The external preparation of claim 9, wherein the skin condition is at least one selected from the group consisting of skin wounds, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, weak rash, and acne composition. Yellow Chlorella (chlorella) extract; and
As a pharmaceutical composition for the prevention or treatment of inflammatory diseases, including; glucose, oligosaccharides, and sugars including fructose,
A pharmaceutical composition comprising 5 to 30% by weight of yellow chlorella extract, 10 to 30% by weight of glucose, 10 to 30% by weight of oligosaccharides, and 15 to 20% by weight of fructose, based on the total weight of the composition. The method according to claim 11, wherein the inflammatory disease is dermatitis, allergy, atopic dermatitis, conjunctivitis, periodontitis, rhinitis, otitis media, sore throat, tonsillitis, pneumonia, gastric ulcer, gastritis, Crohn's disease, colitis, arthritis, ankylosing spondylitis, tendinitis, tendinitis, peritenitis, Myositis, hepatitis, cystitis, and any one or more pharmaceutical composition selected from the group consisting of nephritis. Yellow Chlorella (chlorella) extract; and
As a health functional food for improving skin condition, including; sugars including glucose, oligosaccharides, and fructose,
Based on the total weight of the health functional food, yellow chlorella extract is contained in 5 to 30% by weight, glucose is 10 to 30% by weight, oligosaccharide is 10 to 30% by weight, and fructose is 15 to 20% by weight. Health for improving skin condition functional food.

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