KR100795512B1 - Skin care composition comprising the extract of Centella asiatica and Magnolia kobus - Google Patents

Abstract

The present invention relates to a skin protective composition for synergistic antibacterial and skin moisturizing comprising Centella asiatica and Magnolia extract. The composition of the present invention is not only a safe composition having antimicrobial effect, moisturizing effect, and antioxidant effect and not toxic to human skin, but also protects skin showing synergistically superior antimicrobial activity compared to the use of each extract alone. Composition.

Centella, Magnolia, Antibacterial, Moisturizing, Antioxidant

Description

Skin care composition comprising the extract of Centella asiatica and Magnolia kobus

The present invention relates to a skin care composition for antibacterial and skin moisturizing, including centella and magnolia extract.

The skin consists of several layers of cells that coat and protect keratin and collagen fiber proteins that form the framework of the skin structure. In addition, since our skin is in direct contact with the external environment, it is very sensitive to external stimuli and changes, protecting our body's internal environment. However, skin damage is caused by various external or internal stressors, and the skin damage inducing factors include very high oxidizing substances such as free radicals and oxides. Due to these skin damage inducing factors, the lipid layer forming the skin is damaged, and the percutaneous water loss is rapidly increased, which causes skin dryness. In addition to the skin drying phenomenon, infections caused by bacteria or viruses also occur, accompanied by inflammation on the skin. In order to block skin damage caused by such a series of processes, it is necessary to block moisture loss to increase the moisturizing effect, to block the activity of bacteria, and to remove oxidizing substances.

Accordingly, the present inventors have found a composition comprising an extract of centella and magnolia which is excellent in antimicrobial, moisturizing and antioxidant power and is safe for human application, and has completed the present invention.

Therefore, an object of the present invention is to provide a composition for protecting the skin, which has an excellent antimicrobial activity, moisturizing effect and antioxidant effect, and includes centella and magnolia extracts, which are secured in human application.

In addition, an object of the present invention is to provide a skin care food, cosmetics and medicines using the skin care composition.

In one embodiment, the present invention provides a composition for protecting the skin, including centella and magnolia extract.

Skin protection compositions of the invention provide antimicrobial, skin moisturizing and antioxidant effects.

Centella asiatica , which is included in the composition of the present invention, is a perennial plant belonging to the family Apiaceae. Glycosides or derivatives thereof, which are isolated from the centella, are known to be effective for skin ulcers, extensive trauma treatment, skin veins, ulcerative ulcers, erosive skin ulcers, and alopecia areata. In addition, a new material for the treatment of dementia has recently been synthesized therefrom.

Also, a deciduous arborescent of Magnolia (Magnolia kobus) are magnolia and included in the compositions of the present invention, also referred to as a god (辛夷). Magnolia buds and buds are known to be effective in atherosclerosis and sinusitis. The bark is used as a fragrance and is known to be effective against hookworms and headaches.

Prior to the present invention, it has already been known that by using the centella extract on the skin, as described above, there is a wound healing effect. However, it is not known that the centella extract may be used in conjunction with the magnolia extract to synergistically increase the effect of the extract. The present inventors have found that the combination of Centella asiatica and Magnolia extract is synergistically superior to the use of each of them, and the synergistic effect can be further increased by adjusting the compounding ratio.

Thus, in one specific embodiment, the composition of the present invention is a skin care composition for antibacterial and moisturizing skin comprising Centella and Magnolia extract in a ratio of 1: 0.3 to 3. In a more preferred embodiment, the compositions of the present invention comprise centella and magnolia extract in a ratio of 1: 1.5 to 2.5. In even more preferred embodiments, the compositions of the present invention comprise Centella and Magnolia extract in a ratio of 1: 2.

Centella and magnolia extracts included in the composition of the present invention can be extracted using water, an organic solvent or a mixed solvent thereof. All of these components may be mixed and extracted at the same time, or each component may be extracted and mixed under optimum conditions. For example, water is added to the components of the composition of the present invention and heated at a temperature of at least 90 ° C, preferably at least 80 ° C, more preferably at least 70 ° C, for at least 2 hours, preferably at least 1 hour, The mixture can be prepared by filtration. When heating at a high temperature, the time is reduced, but the active ingredient is destroyed and can be lost. When heating at a temperature lower than the temperature, it is important to control the temperature and time properly because the active ingredient of the mixture is not completely extracted. This extract can be dried or used as it is. When extracted using an organic solvent, alcohols such as methanol, ethanol, isoprotanol, butanol, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N-dimethylformamide), DMSO (dimethyl sulfoxide) Side) or a mixture thereof, or the like, to extract at room temperature or by heating. In addition, it can also be extracted by supercritical extraction according to a known method, it may be extracted by combining a variety of known methods for natural products.

As described above, the separated extract is filtered through paper or nylon or the like to remove suspended solid particles, or filtered using a freeze filtration method, and then used as it is or freeze-dried, vacuum dried, hot air dried. , Spray drying can be used for drying.

The extract included in the skin care composition of the present invention is 0.001 to 30% by weight, preferably 0.001 to 20% by weight, more preferably 0.001 to 10% by weight relative to the total weight of the composition.

Centella asiatica extract included in the skin protection composition of the present invention has a wide range of antimicrobial activity (Table 2) and antioxidant effects (Table 1) against various microorganisms, and also has no cytotoxicity (Table 4) to human cell lines, as well as human skin Safe (Table 5) and did not show acute oral toxicity. In addition, in the experiments on the human body, the skin moisturizing power (Table 8) is excellent and the skin protection effect (Table 9) is shown.

The skin protection composition of the present invention may include additional components that do not inhibit the antimicrobial, skin moisturizing, and antioxidant effects thereof in addition to the extract described above.

As further components having antimicrobial properties, conventionally known antibiotics can be used, including natural antimicrobials. As a natural antimicrobial agent, there are tea tree extracts, and tetracycline, metronidazole, amoxicillin, clarithromycin and the like as known antibiotics within the effective content range of the composition of the present invention. It may be prepared by mixing to a content of 50% by weight.

As an additional component, it can be prepared by mixing jade porridge, lily, peony, lotus root and sulphurous extract, which are reported to have a skin moisturizing effect, in an amount of 5 to 50% by weight within the effective content range of the present invention composition.

Forsythia Fructus extract, ascorbic acid, beta-carotene, dibutylhydroxyltoluene (BHT), DL-alpha-tocopherol (Dl-alpha-) reported as antioxidants as additional ingredients tocopherol) and the like can be prepared by mixing in an amount of 5 to 50% by weight within the effective content range of the composition of the present invention.

As described above, the skin protection composition according to the present invention having a broad skin protection effect can be widely used to achieve the purpose of skin improvement in medicine, cosmetics, food and the like. Specifically, for medicines, such as antibiotics or antifouling agents, foods for skin protection health foods, cosmetics and household goods, atopic or psoriasis and general products related to moisturizing, dandruff suppression, athlete's foot prevention, armpit collection It can be used for products directly related to microorganisms such as inhibition, acne, and anti-aging products related to antioxidant. However, the skin protection composition of the present invention is not limited to these products.

Centella as used in the compositions according to the invention are known to have little toxicity and side effects. Therefore, the composition of the present invention can be used in medicine, cosmetics, food, and the like without concern for toxicity and side effects.

Therefore, as one specific embodiment, the present invention provides a pharmaceutical comprising the composition for protecting skin having the antibacterial, antioxidant, and moisturizing effects as an active ingredient.

The medicament contains the composition for skin protection according to the invention as described above in the range of about 1 to 30% by weight, preferably about 1 to 20% by weight, based on the total weight.

The medicine may be formulated as a conventional formulation in the pharmaceutical field according to the purpose of application, for example, tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warnings, ointments, sprays, Oils, gels, spirits, tinctures, baths, linings, lotions, patches, pads, creams and the like. Although it can be preferably used as an external preparation for skin for the purpose of topical application directly applied to the affected area, an ointment, a lotion, a spray, a gel or the like is preferable. These dermal external preparations may also be included in support bases or matrices or the like, which can be applied directly to the treatment site, and examples of the support bases include gauze or bandages. The natural plant extract used in the formulation may be in colloidal form or dried powder form.

When formulated as an ointment, the skin surface temperature, skin pH, transdermal water loss value, epidermal total lipid value, etc. should be taken into account. Vaseline, liquid paraffin, paraffin, plastibase, silicone, lung (lard), vegetable oils, waxes, formulated in combination with oil-based, water-soluble substrates, emulsion bases, suspension bases, such as lanolin. These ointments include antioxidants (e.g. tocopherol, BHA, BHT, NDGA, etc.), preservatives (e.g. phenolic substances, chlorobutanol, benzyl alcohol, parabens, benzoic acid, etc.), moisturizers (e.g. glycerin, propyleneclicone, sorbitol) ), Dissolution aids (eg ethanol, propylene glycol), softening aids (eg liquid paraffin, glycerin, propylene glycol, surfactants, etc.) and the like.

When formulated into a lotion, it may be prepared in a lotion such as a solution, suspension or emulsion type, in the case of a lotion applied to the skin may be prepared to have a viscosity of 200 cps to 500 cps, for example, In order to give a soft feeling, it is preferable to combine a humectant such as glycerin and propylene glycol.

When formulated with a spray, a propellant or the like may be combined with the additives to disperse the dispersed dispersion or wet powder.

When formulated as a patch, absorption accelerators can be used to increase the absorption of the composition through the skin.

The medicine of the present invention can be administered in various dosage forms, such as oral, parenteral, topical administration, rectal administration, intranasal administration.

In the case of oral administration, for example, oral solids, together with the actives, can be diluted (eg lactose, dextrose, sucrose, cellulose, corn starch, potato starch, etc.), suspending agents (eg silica, talc, stearic acid). , Magnesium or calcium stearate, polyethylene glycol, etc.), binders (e.g. starch, arabic gum, gelatin methylcellulose, carboxymethylcellulose, polyvinyl pyrrolidone, etc.), disintegrants (e.g. starch, alginic acid, alginate, Sodium starch glycolate, etc.), dyes, sweeteners, wetting agents (eg, lecithin, polysorbates, laurylsulfate, etc.), and other generally pharmacologically inactive substances used in pharmaceuticals.

When administered topically, excipients (e.g. lactose, starch, cellulose, lactose, polyethylene glycol, etc.), lubricants (e.g. magnesium stearate, stearic acid, glyceryl behenate, talc, etc.), preservatives (e.g. benzylalkonium chloride) And the like). Topical administration includes the treatment of areas or organs of infection that are easily accessible by topical application such as, for example, ear, vagina, open and closed wounds or closed wounds and skin, including eye, outer and middle ear infections. It also includes transdermal delivery resulting in systemic effects.

Parenteral administration includes injections that produce a systemic effect or injections administered directly to the suffering area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intradural, intraocular and general infusion techniques. Rectal administration includes the form of suppositories, and intranasal administration includes nasal aerosol or inhalation applications.

The pharmaceutical product of the present invention is administered in a pharmaceutically effective amount. As used herein, "pharmaceutically effective amount" means an amount sufficient to treat or prevent a disease at a reasonable benefit / risk ratio applicable to medical treatment or prophylaxis, and an effective dose level means the type of disease and its severity; Activity of the drug; The age, body weight, health and sex of the patient; Sensitivity to the drug of the patient; The time of administration, route of administration, and rate of excretion of the drug used; Duration of treatment; Or factors well known in the medical field and other factors, including concurrently used drugs. In general, adults may be administered an amount of 0.1 to 1000 mg / kg per day, preferably a dose of 10 to 100 mg / kg, once to several times daily.

The compositions of the present invention may also be added to foods or beverages for the purpose of protecting the skin.

Therefore, as another aspect, the present invention relates to a functional food comprising the composition for skin protection as described above.

Natural foods such as cereals, potatoes, beans, vegetables, fruits, seafood, meat, milk, and seaweeds; Or processed foods, such as bread, confectionery, ramen, and ice cream; Alternatively, the functional food as described above may be prepared by including the composition of the present invention in a beverage or the like. The amount of extract in the food or beverage may generally be added at 0.01 to 5% by weight of the total weight of the food, and the beverage may be added at a rate of 0.01 to 2 g, preferably 0.3 to 1 g, based on 100 ml.

The beverage to which the skin-protecting composition of the present invention is added has no special limitation except for including the extract of Centella as an essential ingredient in the indicated ratio, and additional ingredients such as various flavors or natural carbohydrates, such as ordinary drinks. It may contain as. Examples of the natural carbohydrate include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Conventional sugars such as dextrin, cyclodextrin, and the like; And sugar alcohols such as xylitol, sorbitol, and erythritol. As said flavoring agent, natural flavoring agents, such as tautin and a stevia extract (for example, rebaudioside A, glycyrrhizin, etc.), and synthetic flavoring agents, such as saccharin and aspartame, can be used advantageously. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the skin care composition of the present invention.

In addition to the above, food and beverages include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH Regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages and the like. Others may contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently. The proportion of such additives is generally selected from the range of 0 to about 20% by weight per 100% by weight of the functional food of the present invention.

The skin protection composition of the present invention may be added directly to foods, but the skin protection effect may be obtained by directly immersing the target food in the skin protection composition or spraying the skin protection composition on the food.

As another aspect, the present invention relates to a cosmetic comprising the composition for skin protection described above.

Skin protection composition included in the cosmetic of the present invention is 0.01 to 20% by weight, preferably 0.01 to 10% by weight, most preferably 0.1 to 3.0% by weight relative to the total weight of the cosmetic.

Ingredients included in the cosmetic of the present invention as an active ingredient includes components commonly used in cosmetics in addition to the above skin care composition. Conventional adjuvants such as, for example, thickeners, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers and the like.

Cosmetics of the present invention can be prepared in any formulation commonly prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, and the like. It is not limited to this. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the cosmetic formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier components. Can be.

When the cosmetic formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the cosmetic formulation of the present invention is a suspension, as a carrier component, a diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the cosmetic formulation of the present invention is a surfactant-containing cleansing agent, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid. Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are only intended to illustrate the present invention in more detail and are not intended to limit the present invention thereto.

Example 1: antibacterial effect

Agar diffusion was used to investigate the antimicrobial activity of Centella asiatica extract. The concentration of Centella asiatica extract was 300 ug / ml, and microbial strains were Gram-positive bacteria, Bacillus subtilis ATCC 6633 ( B. subtilis ), Staphylococcus aureus ATCC 6538P ( S. aureus ), Micrococcus luteus ( M. luteus ) ATCC 9341, Methicillin resistant staphylococcus aureus (MRSA) C2207, gram negative bacteria Escherichia coli (E. coli) ATCC 10536, Escherichia coli (E. coli) 0157: K88ac, Pseudomonas aeroginosa (P. aeroginosa ) ATCC 1636, Klebsiella pneumoniae (K. pneumoniae) ATCC 10031, Candida As Yeast albicans (C. albicans ) ATCC 10231, fungi Aspergillus niger (A. niger ) ATCC 9029, Trichophyton Mentagrophytes (T. mentagrophytes) KCTC 6077, Fusarium solani (F. solani) was tested for antimicrobial activity by agar diffusion method. The results are shown in Table 1.

Microbial strain B. subtilis S. aureus M. luteus MRSA E. coli E. coli 0157: K88ac P. aerosinosa K. pneumonia C. albicans A. niger T. mentagrophyte F. solani Antimicrobial activity +++ +++ ++ ++ + + ++ +++ +++ ++ +++ +++

+: Weak (transparent stand size: less than 2 mm), ++: strong (transparent stand size: less than 2 to 5 mm), +++: very strong (transparent stand size: 5 mm or more)

Example 2 Synergistic Antimicrobial Effects of Centella asiatica and Magnolia Extracts

From the test results of Example 1, it can be seen that the antibacterial efficacy of Centella asiatica extract against E. coli is lower than other strains. In order to increase the antimicrobial efficacy against E. coli , an experiment was performed to determine the composition ratio to maximize synergistic antimicrobial effect by adding magnolia extract known as antimicrobial substance. Agar-diffusion and magnolia extracts were mixed in various ratios, and agar diffusion was used as an antimicrobial test for E. coli .

100 g of dried stem bark of Magnolia (Kyungdong Market) was extracted by immersing in 1 L of 80% ethanol for 48 hours in the dark and at room temperature, and then completely dried with a reduced pressure dryer to obtain dry matter, and 0.1 ml of DMSO in 10 mg of dried matter. In addition, 100 mg / ml stock was prepared and used diluted to suit each test.

As shown in Table 2, it was confirmed that synergistic efficacy was observed when the centellar and magnolia extracts were treated simultaneously than when the centellar extract or magnolia extract alone was treated. In addition, the combination of centella extract and magnolia extract at various ratios showed the highest synergistic antimicrobial effect at a specific combination ratio of 1: 2 (centrum extract: magnolia extract).

extract Antimicrobial Activity against E.coli Centella (150ug / ml) + Magnolia (150ug / ml) [Composition ratio = 1: 1] ++ Centella (100ug / ml) + Magnolia (200ug / ml) [Composition ratio = 1: 2] ++++ Centella (75ug / ml) + Magnolia (225ug / ml) [Composition ratio = 1: 3] ++ Centella (200ug / ml) + Magnolia (100ug / ml) [Composition ratio = 2: 1] ++ Centella (225ug / ml) + Magnolia (75ug / ml) [Composition ratio = 3: 1] ++ Centella (300ug / ml) + Magnolia (300ug / ml) +

+: Weak (transparent stand size: less than 2 mm), ++: strong (transparent stand size: less than 2 to 5 mm), +++: very strong (transparent stand size: 5 mm or more), Centella: centella extract, magnolia: magnolia extract

Example 3 Antioxidant Effect

100 g of dried stems and leaves of Centella asiatica (Gyeongdong Market) were extracted by immersing in 1 L of 80% ethanol for 48 hours in the dark and at room temperature, followed by complete drying with a reduced pressure dryer to obtain dry matter. 100 mg / ml stock was prepared by addition of 0.1 ml, and diluted to suit each test.

100 g of dried stem bark of Magnolia (Kyungdong Market) was extracted by immersing in 1 L of 80% ethanol for 48 hours in the dark and at room temperature, and then completely dried with a reduced pressure dryer to obtain dry matter, and 0.1 ml of DMSO in 10 mg of dried matter. In addition, 100 mg / ml stock was prepared and used diluted to suit each test.

DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to investigate the antioxidant effects of centella, magnolia, or a mixture of centella and magnolia. DPPH is a colored radical that can be used to directly determine the radical removal capacity of a sample. The sample was dissolved in 4 mL of distilled water or solvent (methanol), mixed well with 1 mL of 100 μM DPPH, incubated for 30 minutes at room temperature, and the amount of DPPH remaining was measured for absorbance at 517 nm to determine the antioxidant effect. Distilled water was used for blank test, DPPH and distilled water were used for control, and vitamin C was used for positive control. As a result, the antioxidant activity (Radical Scavenging Activity (RSA)) was determined at a lower absorbance ratio by comparing each hydrolyzate with the absorbance of the control according to Equation 1.

Antioxidant Effect of Centella asiatica Extract Concentration of extract RSA (%) Centella (150ug / ml) + Magnolia (150ug / ml) [Composition ratio = 1: 1] 40 Centella (100ug / ml) + Magnolia (200ug / ml) [Composition ratio = 1: 2] 22 Centella (75ug / ml) + Magnolia (225ug / ml) [Composition ratio = 1: 3] 38 Centella (200ug / ml) + Magnolia (100ug / ml) [Composition ratio = 2: 1] 45 Centella (225ug / ml) + Magnolia (75ug / ml) [Composition ratio = 3: 1] 35 Centella (300ug / ml) 55 Magnolia (300ug / ml) 65 Vitamin C (1 mM) 30 Control 100

As shown in Table 3, it was confirmed that synergistic antioxidant effect appeared when the centella and magnolia extract were treated at the same time than when treated with centella extract or magnolia extract alone. In addition, the combination of centella extract and magnolia extract at various ratios showed the highest synergistic antioxidant activity at a specific combination ratio of 1: 2 (centella extract: magnolia extract).

Example 4: Cytotoxicity

Cytotoxicity experiments (MTT: 3- (4,5-dimetylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) were performed using HaCaT cell culture, a human keratinocyte line. After treatment with HaCaT cells, the cells were cultured in a serum-free state, and the cytotoxic effects of the cells were measured using the MTT assay method. MTT is a pale yellow substrate that is cleaved by respiratory enzymes in the mitochondria of living cells and produces dark blue formazan. Since the reaction does not occur in dead cells, the amount of formazan produced is used to determine the number of living cells. The results are shown in Table 4.

As shown in Table 4, in the case of HaCaT, neither the centella extract nor the magnolia extract showed cytotoxicity at a concentration of 1000 ug / ml or less. In addition, no cytotoxicity was observed at concentrations showing the best synergistic antimicrobial and antioxidant efficacy.

Example 5 Safety to Human Skin

When the cytotoxicity test of Example 4 proves that there is no cytotoxicity, in order to confirm whether or not it is actually safe to human skin, skin safety verification experiments were performed. As a test method, a normal cumulative skin irritation test was performed.

Formulation based on squalene known to be non-irritating to humans, Centella asiatica extract (30 mg / ml), Magnolia extract (30 mg / ml), or Centella asiatica and Magnolia extract 10 mg / ml and 20 mg / ml respectively Was prepared. Centella, magnolia extract was added to squalene, and mixed for 5 minutes at room temperature 3000rpm using a homo. Using the sample prepared as described above, 30 healthy adults were subjected to a total of 9 24-hour cumulative patches on the upper forearm every other day, indicating whether Centella, Magnolia, or Centella and Magnolia extract irritated the skin. Whether or not was measured.

The patching method used a fin chamber (Finn chamber, Epitest Ltd, Finland). 15 µl of each of the external skin preparations was added dropwise to the chamber, and then patched. The degree of response to the skin each time was scored using Equation 2 below, and the results are shown in Table 6 below. At this time, ± 1 point, + 2 points, + + 4 points in the reaction degree, and the average response was determined to be a safe composition when less than 3.

In Table 5, the number of people corresponding to ±, +, and ++ in the control group and test groups 1, 2, 3, and 4 were 2, 0, and 0, respectively. When calculated according to Equation 2 above, since the average reactivity of the test groups 1,2,3,4 are all 0.18 and 3 or less, the test groups 1,2,3 of the present invention do not show a distinct cumulative stimulation pattern and the human skin Was determined to be a safe substance.

Example 6 Preparation of Skin Improvement Cosmetic Composition

Nutrition Cream

Examples of preparation of nutrient cream containing Centella, Magnolia, or Centella and Magnolia extract are shown in Table 6 below. Purified water, triethanolamine, and propylene glycol, which were aqueous phases, were dissolved by heating at 70 ° C. The oily fatty acid, an oily component, an emulsifier, and the preservative were melt | dissolved by adding the liquid which heated and melted at 70 degreeC here. After the emulsification was completed, the solution was cooled to 45 ° C., followed by adding and dispersing centella and magnolia extract and flavor, and then cooling to 30 ° C.

Ingredients and Contents of Nutritional Cream Containing Centella asiatica Extract Content (% by weight) ingredient  Test group 1 Test group 2 Test group 3 Centella extract 3 0 One Magnolia extract 0 3 2 Jojoba oil 4.0 4.0 4.0 Floating paraffin 5.0 5.0 5.0 Cetylaryl alcohol 1.5 1.5 1.5 Polyglyceryl-3 Methyl Glucose Distearate 1.5 1.5 1.5 Glyceryl Stearate 0.5 0.5 0.5 Squalane 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 glycerin 6.0 6.0 6.0 Triethanolamine 0.2 0.2 0.2 Carboxy Vinyl Polymer 0.2 0.2 0.2 Tocopheryl acetate 0.3 0.3 0.3 Preservative, incense a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Sum 100 100 100

compare Production Example  : Purified water  Cream containing

In the preparation example of the nutrition cream containing the centella extract, the composition of the cream substitute with purified water without adding centella and magnolia extract is as follows.

ingredient Content (% by weight) Jojoba oil 4.0 Floating paraffin 5.0 Cetylaryl alcohol 1.5 Polyglyceryl-3 Methyl Glucose Distearate 1.5 Glyceryl Stearate 0.5 Squalane 2.0 Propylene glycol 2.0 glycerin 6.0 Triethanolamine 0.2 Carboxy Vinyl Polymer 0.2 Tocopheryl acetate 0.3 Preservative, incense a very small amount Purified water Remaining amount Sum 100

Example  7: moisturizing effect

The degree of transepidermal water loss (TEWL) was measured using a TEWA (Tewameter TM210, Germany) evaporator. Measurements were performed by treating the centella extract alone, magnolia extract alone, centella and magnolia extract mixed cream and a cream containing no sample at all, and performed once every 4 weeks, and then statistically compared. All measurements were taken in a constant temperature and humidity room with no air movement, no direct sunlight, and a room temperature of 22 ° C and a relative humidity of 40%. In 30 subjects, the measurement site was the abdomen. As shown in Table 8, in case of nutritive cream containing centella extract, magnolia extract, centella and magnolia mixed extract, all moisture loss was reduced. In particular, the nourishing cream of Centella asiatica extract (10 mg / ml) and Magnolia extract (20 mg / ml), it was found that the moisturizing effect is superior to the Centella asiatica or Magnolia extract alone. In other words, if the composition ratio of Centella extract and Magnolia extract is 1: 2. It can be seen that there is also a synergistic effect on the skin moisturizing effect.

Example  8: skin improvement test

SDS (0.01%) irritates the skin and damages the skin tissue, and then applies the nourishing cream containing Centella asiatica, Magnolia extract, or a mixture of Centella asiatica and Magnolia extract to the upper forearm area to see if the skin tissue is improved. Observed. In this experiment, 30 healthy adults were included.

The patching method used a fin chamber (Finn chamber, Epitest Ltd, Finland). 15 μl of each of the external skin preparations was added dropwise to the chamber, followed by patching. The degree of response to the skin each time was scored using Equation 3 below, and the results are shown in Table 9 below.

At this time, ± 1 point, + 2 points, + + 4 points in the reactivity was given.

Test substance Responsiveness Average responsiveness 1 week 2 weeks 3 weeks Control group (squalene) 0.10 0.14 0.14 0.127 SDS (0.01%) 4.5 4.3 4.1 4.3 Centella asiatica Extract (3%) Nourishing Cream [Test Group 1] 3.7 2.1 1.7 2.50 Magnolia Extract (3%) Nourishing Cream [Test Group 2] 3.3 2.0 1.5 2.26 Centella asiatica Extract (1%) + Magnolia Extract (2%) Nourishing Cream [Test Group 3] 0.86 0.75 0.67 0.76 Centella asiatica Extract, Magnolia Extract (0%) Nourishing Cream [Comparative Example] 4.4 4.0 4.1 4.17

As shown in Table 9, when the SDS (0.01%) is applied to the skin, it can be seen that the average reactivity is severely damaged skin. On the other hand, when applying the test groups 1, 2, 3, respectively, it was confirmed that there is an effect to improve the skin. In particular, it was found that the nutritive cream containing Centella extract (1%) and Magnolia extract (2%) had better skin improvement effects than Centella extract (3%) or Magnolia extract (3%) alone. In other words, if the composition ratio of Centella extract and Magnolia extract is 1: 2. It can be seen that there is a synergistic effect on the skin improvement effect.

Example 9: Acute Oral Toxicity Test

Acute oral toxicity test was conducted to confirm whether the centella extract and magnolia extract of the present invention are non-toxic. Twenty five to six week old SPF SD rats were used as an experimental animal under the acute toxicity test under the following conditions.

Temperature and humidity conditions: temperature 22 ℃ ± 2 ℃. Relative Humidity 50% ± 10%

Contrast cycle: Fluorescent light illumination (lights off at 8:00 after lighting at 8:00)

Illuminance: 200-300 Lux

Drinking free UV water

In the test group, test substances were diluted and administered in sterile distilled water, and in the control group, sterile distilled water was administered in the same amount.

On the day of administration, the general condition is observed every hour up to 4 hours, and once a day from the day after the administration, the general condition changes, poisoning symptoms, mobility, appearance, autonomic nerves, and the presence of dead animals are carefully observed. Pathological anatomical verification was performed to determine the toxicity. LD ₅₀ was 2800 mg / kg BW for centellae and 2750 mg / kg BW for magnolia. Therefore, both centella and magnolia extracts were judged to be non-toxic.

Skin protection composition comprising Centella asiatica and Magnolia extract according to the present invention is a useful substance having antibacterial, moisturizing and antioxidant effects at the same time can be widely used in skin protection, synergistically compared to the use of each extract alone It is a skin protection composition showing excellent antimicrobial activity.

Claims (6)

Skin care composition for antibacterial and skin moisturizing including centella and magnolia extract in a ratio of 1: 0.3 to 3. The composition of claim 1 comprising centella and magnolia extract in a ratio of 1: 1.5 to 2.5. The composition of claim 2 comprising centella and magnolia extract in a ratio of 1: 2. The composition of claim 1, wherein the extract of the centella and magnolia is included in an amount of 0.001 to 30% by weight relative to the total weight of the composition. An antibacterial and skin moisturizing product comprising the composition of claim 1. 6. The product of claim 5, wherein said product is a cosmetic.