KR101763373B1 - Cosmetic composition comprising plant extracts as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a plant extract as an active ingredient. According to the present invention, it is possible to provide an antimicrobial / anti-inflammatory and anti-aging effect promptly and continuously by promoting transdermal absorption effectively, Thus, it is expected to be useful as a cosmetic raw material of a multifunctional function.

Description

[0001] The present invention relates to a cosmetic composition comprising plant extracts as active ingredients,

TECHNICAL FIELD The present invention relates to a cosmetic composition comprising a plant extract as an active ingredient, and more particularly to a technique for expressing the enhanced efficacy and effect of a plant extract and stabilizing the same.

The human skin undergoes changes due to various internal and external factors as it gets older. Among these, changes caused by internal factors (endogenous aging, natural aging) decrease the secretion of various hormones that regulate metabolism, decrease the functions of immune cells and the activities of cells, and biosynthesis of immune proteins and bioproteins . In addition, changes due to external factors (photoaging) are caused by an increase in the amount of ultraviolet rays in the sunlight due to the destruction of the ozone layer and an increase in free radicals and harmful free radicals as the environmental pollution is further intensified.

Various functional products such as antioxidant, wrinkle improvement, whitening, antimicrobial and anti-inflammation promotion have been proposed in order to suppress such changes, and various studies for the complexation of various functionalities have been conducted.

One example of a functional cosmetic raw material currently in widespread use is an antioxidant material. Vitamin E derivatives, vitamin C and flavonoids are poor in stability and are hardly used as cosmetic raw materials. In addition, BHA (butylated hydroxyl anisol) and BHT (butylated hydroxyl toluene) have been used more than chemically synthesized cosmetic raw materials that are economical and stable. However, recent studies have reported that there is a side effect in long- .

Therefore, research on finding natural antioxidants that are harmless to humans and have no side effects when using for a long time has been steadily carried out, but most of them have not reached the expectation.

For example, recently, various functional cosmetics have been developed, and a lot of raw materials such as AHA, retinol, and arbutin have been developed and used. However, these raw materials are known to cause various side effects such as inflammation, rash, swelling and the like.

As another example, studies have been actively conducted on raw materials that are natural extracts that do not cause side effects to skin and have antioxidative effects, but functional materials that have antioxidative effects with excellent stability without causing side effects are presented It is a fact that it does not become.

Accordingly, the present inventors have repeatedly studied various plant extracts, and found that the combination of specific plant extracts exhibits remarkable efficacy in inhibiting skin aging. In addition, it has been confirmed that by applying a self-assembling polyion complex to the combination of plant extracts according to the present invention, the body stability can be imparted with no irritation, and the transdermal absorption of the active ingredient can be promoted and its efficacy can be increased Thus completing the present invention.

It is an object of the present invention to provide a cosmetic composition which can enhance the stability of a combined plant extract and maximize skin care effect.

It is still another object of the present invention to provide a cosmetic composition excellent in anti-aging properties without cytotoxicity.

It is still another object of the present invention to provide a cosmetic composition excellent in anti-inflammatory and antibacterial effects.

In order to attain the above object, the present invention is characterized in that it comprises a hyssop extract, an acidified flower extract, an Elder flower extract, a primrose extract, and a self-assembling polyion complex. At this time, the polyionic complex may be one in which a water-soluble anionic polymer and hydrophobic cation monomers are ionically bonded.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to solve problems such as crystal precipitation of an active ingredient which may occur when different extracts are combined, lowering of stability, and the like.

In addition, the hyssop extract, the acidified flower extract, the Elder flower extract, and the primrose extract described above can not be used in a high content (a solid content of 1 wt% or more based on the total weight of the extract) of each extract due to solubility and the like It has surprisingly been found that the present invention can solve this problem.

The composition according to an embodiment of the present invention may be an antimicrobial cosmetic composition.

The composition according to an embodiment of the present invention may be a cosmetic composition for anti-inflammation.

The composition according to an embodiment of the present invention may be an anti-aging cosmetic composition.

The composition according to an embodiment of the present invention can be formulated into various formulations such as a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder But is not limited thereto.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a multifunctional natural cosmetic composition showing excellence in antibacterial, anti-inflammatory and anti-aging effects.

In addition, the natural extract itself can not easily penetrate into the stratum corneum, and thus it is insignificant in manifesting the efficacy and effect. However, according to the present invention, the percutaneous absorption can be effectively promoted and immediate and lasting effect in the complex function described above can be expected.

Further, according to the present invention, not only the degree of solubilization in the combined plant extracts can be remarkably improved, but also the polyphosphate complex carries a lipophilic active pharmacologically active ingredient in the hydrophobic interior formed by the self-assembly process in an aqueous solution, Function and thus the increased efficacy and effect can be conferred.

The cosmetic composition containing the plant extract according to the present invention as an active ingredient is described below, but unless otherwise defined in the technical terms and scientific terms used herein, those skilled in the art will be able to In the following description, well-known functions and constructions are not described in order to avoid unnecessary obscuration of the present invention.

The present invention provides a cosmetic composition comprising a natural plant extract capable of dramatically improving the stability of an active ingredient having an efficacy and an effect useful for skin and continuously supplying it to the skin, thereby maximizing the skin care effect.

Also, according to the present invention, the transdermal absorption of plant extracts can be effectively increased, and thus the efficacy and effect thereof can be promptly and continuously provided to the skin.

As used herein, the term "anti-aging" is intended to encompass skin irritation, skin irritation, skin cell proliferation and regeneration, prevention or improvement of skin whitening, skin aging and wrinkles, It is a concept that encompasses all of function, antioxidant ability, collagen synthesis promoting ability and moisturizing ability. It can be all effects and effects for skin protection and skin condition improvement. In the present invention, especially, it is excellent in moisture and antioxidant ability, The effect has priority but is not limited to this.

The term "application, " as used herein, refers to contacting a composition according to the present invention to the skin of an individual by any suitable method, thereby aiming to absorb the composition into the skin.

As used herein, the term "prevention" refers to all actions that inhibit or delay the occurrence of wrinkles by application of the composition according to the present invention, and the term "improvement" Refers to all the actions that improve or wrinkle the wrinkle state, that the skin elasticity becomes stronger than before, or slow down the loss of elasticity of the skin due to aging, or it may mean all actions that have advantages relative to the conventional effect.

As used herein, the term "wrinkle " refers to a scarred skin caused by a genetic cause, reduced collagen and elastin present in the skin dermis, and an external environment. "Elasticity" refers to the elasticity exhibited by elastic fibers composed of elastin existing in the dermal layer of the skin.

The term "antibacterial" as used in the present invention means the ability to resist bacteria and means all mechanisms that are performed to defend against the action of microorganisms such as bacteria or fungi.

The term " anti-inflammatory " as used herein means resistance to inflammation. The inflammation is caused by a signal transduction system of the immune system and includes atopic dermatitis, allergic dermatitis, inflammatory skin diseases, Asthma, and the like.

In addition, the extract according to the present invention may be prepared by adding an extraction solvent to at least one selected from the outposts, leaves, roots, fruits, seeds, flowers, stems, It may be extracted. That is, it may be extracted by a method such as cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction, supercritical extraction, etc. using a conventional extraction solvent under ordinary temperature and pressure conditions, But is not limited thereto. The extraction solvent may be, but is not limited to, water, anhydrous or lower alcohol having 1 to 7 carbon atoms, hexane, chloroform, ethyl ether, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, Propylene glycol, and 1,3-propylene glycol. Preferably, the solvent is selected from water, methanol, ethanol, propanol, butanol, and the like. In addition, the extract extracted with the above-mentioned extraction solvent includes all the extracts, fractions and purified products obtained in each step of fractionation or purification, their diluted solution, concentrated solution or dried product, and if necessary, they are subjected to freeze-drying, vacuum drying , Dried by hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like, and further pulverizing the dried extract. Accordingly, the extract may have a liquid phase, a concentrated solid phase, and a lyophilized powder phase, as the case may be, after filtration, an additional step such as drying and grinding.

The cosmetic composition according to one embodiment of the present invention is characterized by comprising a hyssop extract, an acidified flower extract, an Elder flower extract, a primrose extract, and a self-assembling polyion complex. At this time, the polyion complex is formed by ion-binding the water-soluble anionic polymer and the hydrophobic cation monomers, thereby forming a stable particle structure by self-assembly in a water system.

The cosmetic composition according to an embodiment of the present invention exhibits a synergistic effect on anti-inflammatory and antibacterial properties due to the combination of hysosan extract, acid type flower extract, Elder flower extract, and primrose extract. However, It is very difficult to redissolve these active components with a complex of active ingredients which are different from each other. However, according to the present invention, it has been confirmed that not only the crystal precipitation of the active ingredient is inhibited but also the degree of solubilization of each active ingredient can be dramatically improved.

Therefore, solubility of solubilized plant extracts which can not be used at a solubility of 1 wt% or more (weight of the total extract) can be remarkably improved due to the problem of solubility in the past, and long-term stability of the combined plant extracts can be imparted , Cosmetic materials and formulations using the same.

The cosmetic composition according to one aspect of the present invention may include various plant extracts to achieve various functional complexations. Herein, the additional plant extract may be, but is not limited to, a plant extract selected from peppermint extract, melon extract, moxa extract, bitter peppermint extract, Speedwell extract, lemon balm extract and Yarrow extract.

According to an embodiment of the present invention, a self-assembly may be formed in a variety of shapes in a water system depending on the kinds of the water-soluble anionic polymer and the hydrophobic cationic monomer constituting the polyionic complex and the mixing ratio thereof.

The water-soluble anionic polymer may include any one of hyaluronic acid, gamma-polyglutamic acid, sodium-carboxymethylcellulose, and carbomer, or a combination thereof. However, the present invention is not limited thereto, and it is needless to say that other natural or synthetic water-soluble anionic high polymer materials having suitable moisture resistance may be used.

The water-soluble anionic polymer preferably has a weight average molecular weight of 500,000 to 3,000,000 Da, more preferably 1,000,000 to 3,000,000 Da, in view of imparting excellent moisture resistance, but is not limited thereto.

The hydrophobic cationic monomer may be selected from the group consisting of phosphatidylethanolamine, dioloylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dipalmitoylphosphatidylethanolamine and distearoylphosphatidylethanolamine, cetyltrimethylammonium bromide, cetyltrimethylammonium chloride , Dimethyl dioctadecyl ammonium chloride, or a combination thereof. However, the present invention is not limited thereto.

The hydrophobic cationic monomers increase the hydrophobicity of the polyionic complex to assist in the formation of a self-assembly of the polyionic complex in the aqueous solution. In addition, since the electrical repulsion between the human skin and the water-soluble anionic polymer that are negatively charged by the hydrophobic cationic monomer is reduced or canceled, the water-soluble anionic polymer can further improve the moisturizing ability of the polymer, And at the same time, the combined plant extract according to the present invention can impart transdermal penetration and excellence in the expression of the efficacy and effects thereof.

According to one embodiment of the present invention, the polyion complex is prepared by subjecting a lipophilic pharmacologically active ingredient selected from ceramides, retinol, cholesterol, saturated fatty acids having 12 to 18 carbon atoms and unsaturated fatty acids and the like to a polyion complex by self- It can be carried on the inside of the formed hydrophobic. Such a particle structure can be applied to solubilize the above-described fat soluble pharmacologically active ingredient or improve its dispersion stability.

The cosmetic composition according to one embodiment of the present invention comprises 0.1 to 30% by weight of the hysos extract, 0.1 to 30% by weight of the acidified extract, 0.1 to 20% by weight of the Elder flower extract, 0.1 to 20% 20% by weight of the polyion complex and 0.1 to 5% by weight of the polyion complex.

The cosmetic composition according to an embodiment of the present invention may be a water-based cosmetic composition having a visible light transmittance of 70% or more at a wavelength of 400 to 700 nm and having transparency. Preferably, the visible light transmittance (T _{550 nm} ) of the cosmetic composition according to an embodiment of the present invention may be 70 to 90%, more preferably 80 to 90%, but is not limited thereto.

Moreover, one or more plant extracts selected from peppermint extract, melon extract, moxa extract, bitter peppermint extract, Speedwell extract, lemon balm extract and Yarrow extract, etc., can be used to add synergy or impart additional functions in the above- 0.1 to 20% by weight.

Hereinafter, the efficacy and effects of the cosmetic composition according to one embodiment of the present invention will be described in detail.

First, the cosmetic composition according to one embodiment of the present invention has excellent antibacterial and anti-inflammatory activity.

Specifically, the cosmetic composition according to the present invention can be used as a cosmetic composition containing a bacterium such as Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa, and a bacterium such as Candida albicans, Aspergillus niger ) And the like. In particular, it shows excellent antibacterial activity against bacteria such as E. coli and Pseudomonas aeruginosa.

In addition, the cosmetic composition according to one embodiment of the present invention effectively inhibits the production of nitrogen oxides (NO) in cells, and can exhibit an excellent anti-inflammatory effect.

Conventionally, the hapyso extract has anti-inflammatory effect as well as antioxidant effect. However, according to the present invention, the hapyso extract has remarkably improved anti-inflammatory effect compared with the case of using the hapyso extract alone. In particular, according to the present invention, transdermal absorption is promoted, and an immediate and lasting effect can be expected in the above-mentioned effects and effects.

Next, the cosmetic composition according to one embodiment of the present invention has an excellent anti-aging effect. In the present invention, the anti-aging may be represented by the improvement of the moisturizing and / or antioxidant ability, but may include all the effects and effects for skin protection and skin condition improvement.

The cosmetic composition according to one embodiment of the present invention shows excellent antioxidant ability by free radical scavenging and / or inhibition of active oxygen species.

In addition, the cosmetic composition according to an embodiment of the present invention works together with a water-soluble anionic polymer having excellent water-binding ability, so that water can be held very strongly, and the water and the combined plant extract according to the present invention can be strongly It can be released continuously after percutaneous absorption to maximize anti-aging effect.

Next, the cosmetic composition according to one embodiment of the present invention has an excellent skin soothing effect. Thus, when the cosmetic composition according to the present invention is applied to the skin, not only the erythema reduction but also the oil content can be effectively controlled and the moisturizing effect can be maximized to help calm the skin condition and improve the sensitivity at the same time.

It is needless to say that the cosmetic composition according to an embodiment of the present invention may further include various additives in addition to the cosmetic composition. The additive may be at least one selected from the group consisting of stabilizers, emulsifiers, thickeners, moisturizers, liquid crystal film stabilizers, pH adjusters, antibacterial agents, water soluble polymers, encapsulating agents, sequestering agents, amino acids, organic amines, polymer emulsions, , An antioxidant auxiliary, a preservative, a perfume, and the like; And one or more oily additives selected from oils, waxes, hydrocarbonaceous oils, higher fatty acid oils, higher alcohols, synthetic ester oils, and silicone oils; And the like.

The aqueous additive is not limited as long as it is a commonly used raw material in the art, and specific examples thereof include glycerin, dipropylene glycol, butyleneglycol, pentylene glycol, methylpropanediol, sorbitol, diglycerin, Erythritol, pentaerythritol, polybutylene glycol-10, polyglycerin-3, polyglycerin-4, polyglycerin-6, polyglycerin-10, polyglycerin-20, polyglycerin-40, sorbes- 6, sorbes-20, sorbes-30, sorbes-40, inositol, maltitol, maltose, mannan, mannitol, mannose, lactitol, lactose, dihydroxypropyl PG-glucoside, dithiaoctanediol, Glucosamine, glucose, 1,2,6-hexanediol, methylglucose-10, methylglucose-20, ozonized glycerin, pyranthiol, thioglycerin, traitol, trimethylol Ol propane, xylitol, (EDTA), guar gum, quinseed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, curdlan, methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, methylhydroxypropylcellulose, chondroitin Sulfuric acid, dermatan sulfate, glycogen, heparan sulfate, hyaluronic acid, sodium hyaluronate, traganth gum, keratan sulfate, chondroitin, mucoitin sulfate, hydroxyethyl guar gum, carboxymethylguanoside, dextran, Carboxymethyl chitin, agar, polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, sodium polyacrylate, polyethylene glycol, bentonite, methyl paraben, propyl paraben , Phenoxyethanol, 1,2-hexanediol, ethylhexyl glycerin, and the like.

The oily additive is not limited as long as it is a raw material generally used in the art, and may be a liquid oil such as olive oil, camellia oil, jojoba oil, triglyceride, glycerin trioctanoate, glycerin triisopalmitate, palm oil, , Solid oils such as hardened oil and hardened castor oil, bees wax, candelilla wax, carnauba wax, lanolin and jojoba wax. Examples of the hydrocarbon flow path include liquid paraffin, squalane, petrolatum, microcrystalline wax and the like. Examples of the higher fatty acids include waxes such as lauric acid, myristic acid, palmitic acid, stearic acid and behenic acid, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol and cetostearyl alcohol And the synthetic ester oils may include isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, hexyl laurate, myristyl myristate, cetyl lactate, isocetylisostearate, neopentyl Higher alcohols such as glycerol dicaprate, ethylhexyl glycerin, cetyl ethylhexanoate, ethylhexyl palmitate and cetostearyl alcohol, chain silicone oils such as dimethylpolysiloxane, methylphenylpolysiloxane and methylhydrogenpolysiloxane, dodecamethylcyclohexa Cyclic silicone oil such as siloxane, octamethylcyclotetrasiloxane and decamethylcyclopentasiloxane, and the like It is, but is not limited thereto.

It should be understood that the formulation according to an embodiment of the present invention may be prepared in the form of a general emulsified formulation and a solubilized formulation or the like, using a conventionally known manufacturing method, in addition to the manufacturing method specifically disclosed in the present invention. In this case, the cosmetic composition may be appropriately selected and formulated according to the purpose. Examples of the cosmetic composition include a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, But may be formulated into a formulation such as a pack, spray or powder, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these embodiments are provided to aid understanding of the present invention, and the scope of the present invention is not limited thereto in any sense.

(Assessment Methods)

1. Storage stability evaluation

Each of the cosmetic compositions prepared in the following examples and comparative examples was placed in a vial having an inner volume of 20 g and placed in a room temperature (25 占 2 占 폚), a cold room thermostat room (4 占 2 占 폚), a high temperature room thermostat room (Freeze-Thaw Chamber, circulated at -20 ° C to 45 ° C once a day), and turbidity and crystal precipitation of each sample were visually observed at intervals of one week.

2. Antibacterial test

Staphylococcus aureus ATCC 6538P as gram-positive bacteria, Escherichia coli ATCC 8739 as gram-negative bacteria, Pseudomonas aeruginosa as a bacterium were used to measure the antibacterial activity of the cosmetic composition prepared in the following Examples and Comparative Examples. ATCC 9027), Candida albicans ATCC 10231 and Aspergillus niger ATCC 22343 were used as yeast.

Approximately 50 μl of each of the cultivated strains was added to a solidified plate medium, and the mixture was dispensed into a plate culture medium and uniformly smoothed using a spreader. About 40 μl of the antimicrobial composition was absorbed into a paper disk having a diameter of 10 mm, the solvent was dried, the microorganism was closely adhered to the surface of the medium on which the strain was coated, and cultured for 48 hours or more in an incubator at 30 ° C. And the size of the clear zone formed in the test piece was measured to confirm the antibacterial effect. Since microorganisms can not grow near the paper disk, the larger the average diameter value, the better the antimicrobial activity. In addition, the same method was used as a positive control, using dimethyl sulfoxide (DMSO), propanediol (PD), and methyl paraben (MP).

3. Anti-inflammatory test

RAW 264.7 cells, a mouse macrophage cell, were treated with LPS (Lipopolysaccharide), an inflammation inducing substance, to confirm inflammation inhibitory effect. Cells were seeded at 2 × 10 ⁵ cells per well in a 96-well plate and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. Overnight and replaced with fresh medium. LPS (1 ug / mL) and each of the cosmetic compositions prepared in the following examples and comparative examples were each administered at different concentrations and cultured for 24 hours. After incubation, the supernatant was taken and centrifuged at 13,000 rpm for 3 minutes. The supernatant was collected, reacted with Griess reagent 1: 1 and absorbance was measured at 570 nm.

4. Antioxidant activity test

Free radical scavenging activity was measured to confirm antioxidant activity. The free radical scavenging activity was measured using a DPPH (1,1-diphenyl-2-picryl-hydrazyl) assay and L-ascorbic acid was used as a positive control. The DPPH itself is a very stable free radical, a violet compound that exhibits characteristic light absorption at 517 nm, and is stable against alcohol and decolorized by a proton-radical scavenger during the antioxidation process, It can be easily observed with the naked eye. In addition, IC ₅₀ means the concentration of the extract required to remove 50% of the free radicals of the no-added control group.

5. Short-term moisturizing test

The dermatologist's physical examination and questionnaires were conducted to select 10 persons who met the selection criteria and did not qualify as exclusion criteria. The subjects were selected 24 hours after the completion of the evaluation, Skin moisturizing sustainability evaluation was performed.

The test site (forearm) is the left or right arm of the subject, or the side of the front ophthalmic cranium 5 cm from the wrists of both arms, and a 1 cm circle is drawn at 1.5 cm intervals for each test site. Prior to instrument evaluation using a noninvasive measuring device, subjects waited for 30 minutes in a waiting room under constant temperature and humidity conditions (temperature 20 ° C, humidity 40%). After 30 minutes, the researchers measured the test site five times with a coneometer (CM825, Courage & Khazaka, Germany) and the average of the three measurements, excluding the maximum and minimum, was determined. After the evaluation of the device, each of the cosmetic compositions prepared in the following Examples and Comparative Examples was applied to the test site of the subject at 2 mg / cm 2, sufficiently absorbed, and allowed to stand at constant temperature and humidity. After 30 minutes, 1 hour, 2 hours, 4 hours, 6 hours and 24 hours after application of the samples, each test site was measured by the same method.

6. Cytotoxicity test

Raw264.7 and B16 / F10 cells were cultured in DMEM (Lonza) medium containing 1% penicillin / streptomycin (GIBCO) and 10% fetal bovine serum (GIBCO) Were cultured in a thermostat at 37 ° C and 5% CO ₂ . At this time, the cytotoxicity was performed using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) method. Specifically, the viability of cells was measured by measuring the absorbance at 570 nm by dissolving Formazan produced by reacting with the enzyme in mitochondria of cells in dimethyl sulfoxide (DMSO).

7. Human skin test

In order to evaluate the effect of the skin soothing composition of the present invention on the skin soothing and sensitivity improving effect, 40 patients (10 patients in Example 1, 10 patients in Example 2, 10 patients in Comparative Example 1 and 10 patients in Comparative Example 2) Were randomly assigned to use for 4 weeks each. Each subject was subjected to skin erythema, oil and moisture measurements before and 4 weeks after use.

Skin erythema was measured using a colorimeter (Chromameter CR-300, Minolta, Japan). When skin tone is measured by a color difference meter, it is represented by L * a * b * three dimensions. Erythema is evaluated by a * value which is a value between a passive value and a negative value. At this time, the higher the a * value, the more severe the skin erythema was evaluated.

Skin fat was measured in the T zone once for 25 seconds using a sebum analyzer (Sebumeter SM810, C + K Electronic Co., Germany). At this time, the higher the value, the higher the skin oil content was evaluated.

Skin moisture was measured using a moisture meter (Corneometer CM820, C + K Electronic Co., Germany) three times and the mean value was used. At this time, it was evaluated that the higher the value, the higher the moisture content of the skin.

(Example 1)

Step 1. Preparation of hyssop extract

The pulverized hyssop of 100 g of dry weight was heated and refluxed with 70% (v / v) ethanol aqueous solution for 12 hours, cooled for one day, and then filtered through a filter paper having a size of 1.2 탆 penetration. Thereafter, the ethanol was removed by distillation under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.5% by weight.

Step 2. Production of acidified flower extract

The pulverized acidified powder having a dry weight of 100 g was heated and refluxed with an aqueous 70% (v / v) ethanol solution for 12 hours, cooled for one day, and then filtered through a filter paper having a throughput size of 1.2 탆. Thereafter, the ethanol was distilled off under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.3% by weight.

Step 3. Manufacture of Elder Flower Extract

The pulverized elder flower having a dry weight of 100 g was heated and refluxed with an aqueous 70% (v / v) ethanol solution for 12 hours, cooled for one day, and then filtered with a filter paper having a permeation size of 1.2 탆. Thereafter, the ethanol was distilled off under reduced pressure, and dissolved in distilled water so that the solid content became 0.25% by weight.

Step 4. Preparation of primrose extract

Flowers and leaves of 100 g of dried ground candles were heated and refluxed with 70% (v / v) ethanol aqueous solution for 12 hours. After cooling for one day, the leaves were filtered with a filter paper having a size of 1.2 μm. Thereafter, the ethanol was removed by distillation under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.5% by weight.

Step 5. Preparation of polyion complex (A)

1 g of hyaluronic acid was dissolved in 90 g of water with stirring. 30 mg of phosphatidylethanolamine was dissolved in 10 g of ethanol, and the resultant was mixed with the above solution of hyaluronic acid. Ethanol was distilled while stirring at 70 DEG C for 2 hours.

Step 6. Preparation of cosmetic composition

Each of the extracts prepared in the above steps 1 to 4 and the polyion complex prepared in the step 5 were mixed in the composition shown in Table 1 to prepare a cosmetic composition.

The storage stability was evaluated by the above-mentioned method using the cosmetic composition prepared by the above method, and the results are shown in Table 2 below. In addition, the efficacy and effectiveness of the cosmetic composition prepared by the above method were evaluated by the above-described method, and the results are shown in Tables 3 to 8.

(Example 2)

Step 1. Preparation of polyion complex (B)

10 g of 300 mg of ceramide DS-Y30 was dissolved in 1,3-butylene glycol heated to 70 占 폚. Then, the polyimide complex solution prepared in Step 5 of Example 1 was added and stirred at 70 ° C. for 2 hours to prepare a solution of the ceramide DS-Y30 in a solution of physically carried on the hyaluronic acid-phosphatidylethanolamine polyion complex Particles were prepared.

Step 2. Preparation of cosmetic composition

Except that the polyion complex of step 1 was used instead of the polyion complex prepared in step 5 of Example 1, to prepare a cosmetic composition.

The storage stability was evaluated by the above-mentioned method using the cosmetic composition prepared by the above method, and the results are shown in Table 2 below. In addition, the efficacy and effectiveness of the cosmetic composition prepared by the above method were evaluated by the above-described method, and the results are shown in Tables 3 to 8.

(Example 3-5)

Were mixed with the composition shown in Table 1 below to prepare a cosmetic composition. The method of producing each plant extract is as follows.

The storage stability was evaluated by the above-mentioned method using the cosmetic composition prepared by the above method, and the results are shown in Table 2 below. In addition, the efficacy and effectiveness of the cosmetic composition prepared by the above method were evaluated by the above-described method, and the results are shown in Tables 3 to 8.

Step 1. Production of peppermint extract

The pulverized mint having a dry weight of 100 g was heated and refluxed with an 80% (v / v) aqueous methanol solution for 12 hours, cooled for one day, and then filtered through a filter paper having a permeation size of 1.2 탆. Thereafter, the methanol was distilled off under reduced pressure, and dissolved in distilled water so that the solid content became 0.2 wt%.

Step 2. Preparation of melo extract

The pulverized melon outpowder having a dry weight of 100 g was heated and refluxed with 100% ethanol for 12 hours, followed by cooling for one day, followed by filtration with a filter paper having a permeation size of 1.2 탆. Thereafter, the ethanol was distilled off under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.15% by weight.

Step 3. Preparation of Lepidoptera

100 g of dried mallow leaves were heated and refluxed for 12 hours in a 100% aqueous solution, cooled for 1 day, and filtered through a filter paper having a pore size of 1.2 mu m (solid content 0.2% by weight).

Step 4. Manufacture of Speedwell extract

The pulverized speed wells having a dry weight of 100 g were heated and refluxed with a 70% (v / v) aqueous ethanol solution for 12 hours, cooled for one day, and then filtered with a filter paper having a permeation size of 1.2 탆. Thereafter, the ethanol was removed by distillation under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.5% by weight.

Step 5. Preparation of lemon balm extract

The pulverized lemon balm having a dry weight of 100 g was heated and refluxed with an aqueous 70% (v / v) ethanol solution for 12 hours, cooled for one day, and then filtered with a filter paper having a permeation size of 1.2 탆. Thereafter, the ethanol was distilled off under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.15% by weight.

Step 6. Manufacture of Yarrow extract

The pulverized Yarrow having a dry weight of 100 g was heated and refluxed with an aqueous 70% (v / v) ethanol solution for 12 hours, cooled for one day, and then filtered through a filter paper having a permeation size of 1.2 탆. Thereafter, the ethanol was distilled off under reduced pressure, and dissolved in distilled water so as to have a solid content of 0.15% by weight.

(Comparative Example 1)

The composition of the following Table 1 was mixed to prepare a cosmetic composition, except that the polyion complex (A) was not used in Example 1.

(Comparative Example 2)

The composition of the cosmetic composition was prepared by mixing the acidified flower extract, elder flower extract, primrose extract, peppermint extract and 1% solution of hyaluronic acid in the composition shown in Table 1 below.

(Comparative Example 3)

The composition of the cosmetic composition was prepared by mixing the hyssop extract, elder flower extract, primrose extract, peppermint extract, melon extract, moxa extract, speedwell extract, lemon balm extract and Yarrow extract according to the composition shown in Table 1 below.

Storage stability was evaluated by the above method using the cosmetic compositions prepared by the methods of Comparative Examples 1 to 3, and the results are shown in Table 2 below. In addition, the efficacy and effectiveness of the cosmetic composition prepared by the above method were evaluated by the above-described method, and the results are shown in Tables 3 to 8.

Example Comparative Example One 2 3 4 5 One 2 3 Hyssop extract 30 30 30 30 30 30 - 30 Acidified extract 30 30 30 30 30 30 30 - Elder flower extract 20 20 20 20 20 20 20 20 Primrose extract 15 18 7 7 7 20 15 15 Polyion complex A 5 - - - - - - - B - 5 5 5 5 - - - Hyaluronic acid - - - - - - 5 - Peppermint extract - - 8 - - - 30 5 Melo extract - - - 5 - - - 5 Moxa extract - - - 3 - - - 5 Speedwell extract - - - - 5 - - 5 Lemon balm extract - - - - 2 - - 5 Yarrow extract - - - - One - - 5 Sum 100 100 100 100 100 100 100 100

Example (1 week / 2 weeks / 3 weeks / 4 weeks) Comparative Example (1 week / 2 weeks / 3 weeks / 4 weeks) One 2 3 One 2 3 Room temperature ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ◎ / ○ / ○ / ○ ◎ / ◎ / ◎ / ○ ◎ / ◎ / ○ / ○ Cold temperature chamber ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ◎ / ○ / ○ / △ ◎ / ◎ / ◎ / ○ ◎ / ◎ / ◎ / ○ High temperature chamber ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ Δ / Δ / × / × ◎ / ○ / Δ / Δ ◎ / ○ / △ / × Cycle chamber ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ◎ / ◎ / ◎ / ◎ ○ / Δ / × / × ◎ / ○ / Δ / Δ ◎ / △ / △ / × ◎: stable; No change in crystal precipitation or turbidity
?: Relatively stable; Turbidity change observed (opaque)
B: Relatively bad; Fluctuation of turbidity is severe (floatation occurs)
X: very bad; Show crystal precipitation

As shown in Table 2, the cosmetic composition according to the present invention was found to have remarkable storage stability with respect to the temperature of Comparative Example. This phenomenon is due to the fact that the active ingredients of different plant extracts effectively inhibit the floating or precipitation of crystals into a complex, which is expected to be a synergistic effect due to the combination of the polyion complex of the present invention and each extract.

division S.aureus E. coli P. aeruginosa C. albicans A. niger Example 1 22.34 20.15 12.48 26.51 21.67 DMSO 6.12 6.43 6.35 6.27 6.84 Propanediol 6.23 6.34 6.75 6.82 6.31 Methyl paraben 13.58 27.15 18.94 40.52 23.48

As shown in Table 3, the cosmetic composition according to the present invention showed excellent antibacterial activity. Especially, it was confirmed that it exhibits excellent antimicrobial activity against E. coli and P. aeruginosa.

division Inhibitory effect on nitrogen monoxide formation (%) Positive control group 50 Example 1 48 Example 2 49 Example 3 53 Example 4 53 Example 5 55 Comparative Example 1 43 Comparative Example 2 38 Comparative Example 3 45 * Positive control:
L-NMMA (L-NG-Monomethylarginine) 10 mg / ml
* Suppression of nitrogen monoxide production (%)
= 1- (Nitrogen monoxide formation amount upon sample addition / Nitric oxide formation amount upon sample addition) x 100

As shown in Table 4 above, it was confirmed that the cosmetic composition according to the present invention showed the ability to inhibit the production of nitrogen monoxide equal to or more than that of the positive control group. When the hyssop extract, the acidified flower extract, the elder flower extract, and the primrose extract were used alone, the inhibitory effect on nitric oxide production was 30%, 21%, 11% and 8%, respectively. The synergistic effect of the cosmetic composition according to the present invention on the inhibitory effect on nitrogen monoxide formation is remarkable when the plant extract obtained by combining these extracts with two kinds is similar to the inhibitory effect on the nitrogen monoxide formation (30%) of the hyssop extract can do.

division IC ₅₀ Ascorbic acid 0.0007 Example One 0.0022 2 0.0020 3 0.0016 4 0.0015 5 0.0013 Comparative Example One 0.037 2 0.051 3 0.042

As shown in Table 5, it was confirmed that the cosmetic composition according to the present invention exhibits a slightly higher free radical scavenging ability than the comparative example, though it has a somewhat higher IC ₅₀ value than ascorbic acid. In addition, the cosmetic composition according to the present invention further comprises at least one plant extract selected from peppermint extract, melon extract, moxa extract, speedwell extract, lemon balm extract, and shallow extract, see.

division Short-term moisturizing test result (%) Example 1 80 Example 2 90 Example 3 95 Example 4 98 Example 5 97 Comparative Example 1 30 Comparative Example 2 45 Comparative Example 3 33 * Short-term moisturizing test result
= (Example # 24 after lapse of time-Immediately after application of the sample) / Immediately after application of the sample * 100
= (Comparative Example # 24 after elapse of time-Immediately after application of the sample) / Immediately after application of the sample * 100

As shown in Table 6, the cosmetic composition according to the present invention showed a short-term moisturizing of not less than 80% in all. This corresponds to an improved moisture retention of 56% or more even in the case of Comparative Example 2 containing hyaluronic acid. The cosmetic composition according to the present invention exhibits remarkable improvement in moisture resistance as compared with Comparative Example.

division IC ₅₀ (%, W / V) Sodium lauryl sulfate (SLS) 0.005 Example One 5.0 2 5.3 3 5.1 4 5.1 5 5.0 Comparative Example One 3.0 2 1.2 3 0.1

As shown in Table 7, the cosmetic composition according to the present invention had an IC ₅₀ value of 5.0 or more and a cytotoxicity of 1000 times or more than that of negative control sodium lauryl sulfate and 50 times or less of that of Comparative Example. That is, it was confirmed that the cosmetic composition according to the present invention is an excellent material with excellent cytotoxicity and excellent stability.

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 four
for
I'm 4
week
after side
anger
rate
(%) four
for
I'm 4
week
after side
anger
rate
(%) four
for
I'm 4
week
after side
anger
rate
(%) four
for
I'm 4
week
after side
anger
rate
(%) Skin erythema
(a _* ) 16.75 12.34 26.32 16.22 12.71 21.63 15.97 14.75 7.63 16.01 15.88 0.81 Oil
(占 퐂 / cm2 占)) 108.2 88.7 18.02 106.7 87.3 18.18 103.3 68.7 33.49 106.2 75.1 29.28 moisture
(AU) 22.4 78.3 249.5 23.1 80.3 247.6 22.8 48.1 110.9 23.4 56.7 142.3

As shown in Table 8, it was confirmed that the cosmetic composition according to the present invention helps skin erythema, oil and moisture in comparison with the comparative example. From this, it is expected that when the cosmetic composition according to the present invention is applied to the skin, skin erythema is relieved effectively, the amount of oily secretion is controlled, the moisture content is increased, and skin relaxation and sensitivity are excellently improved.

Claims (10)

Including hisop extract 0.1 to 30% by weight, dispersed in flower extract of 0.1 to 30% by weight, 0.1 to 20% by weight of elder flower extract, 0.1 to 20% by weight of primrose extract and 0.1 to 5% by weight of a magnetic poly-ion complex assembly is possible, and Wherein the polyion complex is ionically bound to a water-soluble anionic polymer and hydrophobic cationic monomers. The method according to claim 1,
Wherein the cosmetic composition further comprises at least one selected from the group consisting of mint extract, melon extract, moxa extract, bitter mint extract, Speedwell extract, lemon balm extract and Yarrow extract. The method according to claim 1,
Wherein at least one selected from ceramides, retinol, cholesterol, saturated fatty acids having 12 to 18 carbon atoms and unsaturated fatty acids is carried inside self-assembled particles of the polyion complex. delete The method according to claim 1,
Wherein the composition has a visible light transmittance of 70% or more at a wavelength of 400 to 700 nm. The method according to claim 1,
Wherein the composition is antimicrobial. The method according to claim 1,
Wherein the composition is for anti-inflammation. The method according to claim 1,
Wherein the composition is for moisturizing or antioxidizing. The method according to claim 1,
Wherein the composition is for skin soothing. The method according to claim 1,
Wherein the composition is formulated into a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder formulation.