KR20190042883A - Composition for skin and fur of companion animal - Google Patents

Abstract

The present invention relates to a hair/fur care composition for companion animal, which has an effect for skin moisturizing improvement or scalp irritation alleviation, comprising: a natural composite including an extra-cellular matrix (ECM), beta glucan, and silk peptide; and a natural product composite extract including an extract of a licorice, a Houttuynia cordata, a beefsteak plant, and a green tea.

Description

[Composition for skin and fur of companion animal]

The present invention relates to a composition for the care of a feline of a companion animal, and relates to a composition for a companion animal hair follicle composition based on a natural component for improving a skin moisturizing factor, a protective barrier, and a hair.

Skin hygiene, the first barrier to the external environment of the body, is very important to the health of companion animals. However, frequent bathing and washing with a surfactant-containing bath solvent destroys skin moisturizing factors and protective barriers, which are possessed by companion animals, and various skin diseases may occur. In addition, animals with atopic, psoriatic, contact dermatitis, or sensitive skin disease can cause irritation due to dryness even after bathing, causing harm to the health of companion animals. As a result, in bathing and cleaning for skin hygiene, bath solvent for companion animals contains many surfactant components, which may cause serious side effects when applied to long-term skin.

In recent years, development of bath solvents using natural materials has been promoted, and in the case of humans, skin moisturizers and the like have been actively developed as bath additives using natural materials. As a prior art related thereto, a patent application was filed as a "human shampoo composition", "a shampoo composition containing a natural plant extract and a natural herb extract," and a "skin and scalp condition composition containing an extract of a natural product complex fermented extract as an active ingredient".

However, the above prior arts have been developed solely for the purpose of human application, and there is no development of products suitable for skin for companion animals, which is different from human skin.

KR 101478945 B1 KR 101373636 B1

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems of the prior art and the absence of companion animal products, and it is an object of the present invention to combine ingredients capable of stabilizing hair follicles in sensitivity, irritation, And which can remain in the hair follicles for a long time.

It is another object of the present invention to provide a composition for improving skin moisturization and itching of companion animals containing natural products.

The invention relates to natural complexes comprising an extracellular matrix (ECM), beta glucans, silk peptides; And a natural product complex extract containing licorice, lily of the valley, potato, and green tea extract. Preferably, the extracellular matrix (ECM) is extracted by decellularizing and disinfecting endothelial tissue of pig bladder with sodium hypochlorite.

As another example, the present invention provides a composition for managing companion animal follies further comprising a mineral water in the composition. That is, the natural compound, the natural product complex extract and the mineral water are mixed in a weight ratio of 4: 1: 1 as an active ingredient. It is preferred that the mixture comprises 2 to 15% by weight.

In addition, the composition of the present invention has the effect of improving skin moisturizing or fur itchiness, and may be manufactured and provided as a formulation such as a shampoo, a rinse, a conditioner, an essence, a lotion, a treatment, a cleaner and a moisturizer.

The present invention is to provide a composition comprising an effective ingredient of a composition comprising a combination of natural products, which has been found to be effective in skin moisturization, hair gloss and elasticity enhancement, fur itching improvement, skin moisturizing factor and protective barrier.

Further, it has been confirmed that the composition of the present invention is effective as a conditioner capable of administering a bath of a companion animal and a hair care after washing without using a surfactant.

Further, the present invention is a composition for external application for human hair, which is based on a natural ingredient suitable for the skin of a companion animal, has an effect of improving itching and improving atopy.

Figure 1 shows improved results when the finished composition is treated with itching-induced mice.
FIG. 2 shows the results of suppressing the secretion of histamine, which is a cause of itching, when the composition is administered to the peritoneal mast cells separated from the mouse.

Hereinafter, each active ingredient will be specifically described.

(A) Natural complex for skin moisturizing including extracellular matrix

The natural complex for skin moisturizing according to the present invention can be prepared according to the results of previous studies by the present applicant, and the extracellular matrix extracted according to the method described in the '10 dressing agent for treating burns and skin defects and its preparation method', 1020160151332 . The natural complex for skin moisturizing will be briefly described as follows: Extracellular matrix extract, 1% (w / v) aqueous beta-glucan solution, 1% (w / v) hyaluronic acid aqueous solution, 1% (w / v) Silk peptide aqueous solution and marine deep rock mineral mineral water at a weight ratio of 25: 10: 10: 10: 0.66 in an amount of 10 to 12.5% by weight based on the total weight of the total natural skin-moisturizing complex.

The natural complex for skin moisturizing according to the mixing ratio has the effect of extracting collagen and elastin as the main components of the extracellular matrix and enhancing the skin penetration power without peptizing. In addition, since the extracellular matrix includes skin-binding tissue proteins such as elastin and keratin, it is more effective in preventing moisturization and skin aging than when only collagen alone is used. In the present invention, it is possible to maximally increase the penetration of the skin and to maintain the water holding power of the hair follicle continuously, thereby synergistically inducing the effect of improving the itching of the companion animal.

(B) Natural product complex extract

In the composition for skin care of companion animal according to the present invention, the natural product complex extract is prepared by concentrating and producing a mixed extract comprising Sansui oil, Rhodiola, Licorice, Persimmon, Jaso and Green tea extract as an active ingredient . Preferably, the active ingredients of licorice, persimmon, persimmon and green tea are included as much as possible.

Specifically, 1 g of 50% ethanol was added to 100 g of dried and ground liquorice, liquorice, green tea and green tea, and the mixture was firstly extracted in a water bath at 50 ° C for 24 hours. Then, 1 L of 50% . The extracted solution is filtered and concentrated in vacuo to recover about 25 to 30% of the extract.

Recently, the efficacy of various herbal medicines has been reported in human skin, but the problem of cytotoxicity due to administration of one kind of medicinal herb remains a problem to be solved. Therefore, in order to solve these problems, recently, the development of a combination prescription of herbal medicines has attracted great interest, and this approach has the advantage of significantly reducing cytotoxicity.

In the present invention, the above-mentioned licorice extract, Hwasungcho extract, Zygo extract, and green tea extract were selected as candidates and respective studies on the individual extracts were conducted. However, as described above, it has been confirmed that the above-mentioned extract has a problem of cytotoxicity due to the use of a single extract when each individual extract is used alone. Therefore, the use of the individual extract as a conditioner composition for managing animal fingernails due to the problems such as side effects due to the cytotoxicity has a limited use, so that the content of the individual extracts for improving moisturizing and itching is inevitably limited .

Therefore, when a natural medicine extract is mixed with an animal model that induces atopic dermatitis as a combined prescription of the above extracts, the activity of suppressing itching while lowering cytotoxicity is lower than that of using each of the individual extracts alone Respectively.

That is, the natural compound complex extract of the present invention can contain up to 0.2% of each of the whole products of licorice, lobster, potato and green tea. When it is included in the above-mentioned range, it is preferable to use in the above-mentioned range because cytotoxicity is decreased and antioxidant, anti-inflammation and fur itching improving effect can be synergistically induced when used alone or in combination with other combinations.

Here, a brief review of the pharmacological component of the natural product complex extract of the present invention shows that the licorice harmonizes the toxicity of all the drugs to make it appear more effective, regulates the heat and scum of the digestive tract, communicates all blood vessels, . It is known that pharmacological effects are effective for detoxification, hepatitis, urticaria, dermatitis, eczema, etc., and are effective for suppressing peptic ulcers, such as deep-sea germs, muscle relaxation, diuretic action.

Hwasungcho is a perennial plant herbaceous perennial herb that belongs to Saururus chinensis. It has been reported to have antibacterial activity, immunostimulating action, antiinflammatory action, diuretic action, and calming action. Quercitrin, which is a major active ingredient of Rhus verniciflua, has been reported to have various bioactive functions.

Jaso means lobule, and it is known as an effective medicinal agent for antioxidant, anti-inflammation, immune control, sedative, antibacterial, and has a strong anti-inflammatory and immunosuppressive effect on skin and is mainly used for cosmetics and detergents. The effects on hair loss and anti-aging are also known. It helps prevent hair loss by inhibiting enzymes that can cause hair loss. It helps EPA (eicosapentaenoic acid) to strengthen immunity, and exerts excellent effects in improving atopic dermatitis and allergy. It is known.

Catechins extracted from green tea leaves have anti-androgenic properties, so it is very effective to prevent hair loss if they drink green tea frequently. Green tea has the effect of suppressing DHT hormone which causes hair loss lost.

(C) Number of minerals

The mineral water of the present invention is deep ocean rock mineral water, unlike deep ocean water, pure ion mineral is extracted from deep sea rocks rich in minerals, concentrated at a high concentration (20,000 times), stored and diluted according to purpose . Marine deep rock minerals are rich in trace elements such as magnesium, silicon, phosphorus, sulfur, potassium, calcium, vanadium, manganese, iron, cobalt, nickel, copper, zinc, germanium, selenium and molybdenum It replenishes trace elements in minerals. In the composition for improving the skin care or fur itching improvement of companion animals of the present invention, the supply of the deep ocean mineral water to the skin induces the itching-inhibiting effect synergistically, restoring skin immunity, inhibiting itching, natural skin cleansing, antibacterial and moisturizing function ≪ RTI ID = 0.0 > and / or < / RTI >

The components constituting the compositions for companion animal hair care of the present invention may be those that inhibit histamine release, an immune response-related factor of mast cells.

According to one embodiment of the present invention, the composition of the present invention contains the above-mentioned natural complex for skin moisturizing (A), natural extract complex (B) and mineral water (C) in a weight ratio of 4: 1: 1 use. According to the above range, the interaction of the natural complex for skin moisturization, the natural product complex extract and the mineral water can maintain the high fecundity effect of the companion animal in a high level without cytotoxicity.

In addition, in the composition for improving feline care or fur itching of the present invention, the above-mentioned effective ingredient is used so as to contain 14.5% based on the total weight of the composition. If the active ingredient of the natural complex for skin moisturizing (A), the natural product complex extract (B) and the mineral water (C) is contained in an amount of less than 2%, the efficacy thereof is difficult to expect, and if it exceeds 15% Therefore, it is preferable to use it within the above-mentioned range.

According to another embodiment of the present invention, the composition of the present invention may further include vitamin B5 and peppermint oil.

According to another embodiment of the present invention, the present invention includes a composition comprising a composition for improving fur itching.

The cosmetic composition or detergent composition containing the composition of the present invention is most suitable in terms of quality competitiveness and price competitiveness in that the natural complex for skin moisturizing, natural extract compound extract and mineral water are kept in a range of 3 to 4% based on the total weight. In addition, the hair cosmetic base is contained in a range of 96 to 97% based on the total weight, and includes commonly used components such as a natural preservative such as a grapefruit extract, a natural thickener and a natural viscosity regulator, a natural pH regulator, , Natural dyes, natural hair conditioning agents and water.

The composition of the present invention may be formulated into external formulations such as shampoos, conditioners, essences, lotions, sprays, cleaners, and moisturizers, creams, ointments and sticks. In this formulation, the ingredients other than the above-mentioned essential ingredients may be suitably selected and blended by those skilled in the art depending on the type of external preparation, the purpose of use, and the like.

Recently, as the domestic and overseas companion population grows, the market of various products required is growing greatly. The present invention researched and developed peanut management for companion animals for the health of companion animals and companion animals. When developing companion animal preparations, the unexpected toxicity and nonconformity must be fully considered, and as a result, It has been necessary to produce a composition capable of helping to moisturize and relieve itching by using natural ingredients derived from herb medicines and extracellular matrix which are harmless to animals.

The extracellular matrix (ECM), which is a major component, was first discovered about 100 years ago and was initially known to play a structural role as a simple intercellular junction, but beyond that, It plays an important role as a cellular physiological regulator. In particular, the importance of cell therapy and regenerative medicine, which has recently been implicated in embryonic stem cells and adult stem cells, has been emphasized. The extracellular matrix stores and appropriately supplies the biochemical factors necessary for cell growth and differentiation, while providing a physically environment in which the cells can be recognized.

The extracellular matrix is a complex aggregate of biopolymers that fill the tissue or extracellular space and exists in large amounts in the connective tissue, even in small amounts in all tissues. The basement membrane is a kind of extracellular matrix, composed of molecules that are synthesized by cells and secreted and accumulated outside the cells. Examples of the constituent molecules include fibrous proteins such as collagen and elastin, complex proteins such as proteoglycan and glycosaminoglycan, and cell adhesion glycoproteins such as fibronectin, laminin and bitonectin. At present, it is known that cell adhesion activity is also present in collagen and elastic cells.

In the present invention, extracellular matrix is extracted from the endothelium containing the basal membrane, and the matrix fiber is used as a raw material of the pig bladder having excellent biological tissue reconstitativeness and moisturizing properties to fully utilize the characteristics of the extracellular matrix.

In the present invention, the source of the extracellular matrix and the basal fiber is not limited, but it is preferably extracted from the endoplasmic reticulum of the pig, and it may be extracted from the bladder endothelium of animals such as cattle, sheep, deer, and chlorine.

The present invention is primarily aimed at providing a composition for managing companion animal hair, which is not limited to the formulation and can be processed into various formulations.

Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples serve to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

The pig bladder for extracellular matrix extraction described below was purchased from Shimring Co., Ltd., and the silk peptide, hyaluronic acid, and betaclucan were purchased from Kusid BioPharm Co., It is purchased from Bio. Herbal medicines were purchased from Jirisan Medicinal herb.

Manufacturing example  One. Extracellular matrix (ECM) manufacturing

The swine bladder collected is washed well and sterilized by soaking in 500 ml of 10% iodine for 5 minutes. After washing the iodine with sterilized physiological saline, the pig bladder endothelium less than 1 mm in thickness is removed as a white thin film using a surgical knife. Wash three times with aprotonin and 0.1% EDTA in Tris-HCl solution. Then, wash with 0.1% SDS with Tris-HCl solution three times. Separate bladder endothelium is treated sequentially with 40%, 50%, 70% sodium hypochlorite solution at 30-minute intervals for de-aeration and sterilization.

After washing the sodium hypochlorite residue with sterile physiological saline, the reaction is carried out for 3 hours in a Tris-HCL solution containing deoxyribonuclease and ribonuclease for enucleation. It is processed in a dry type high-pressure dry sterilizer at 121 ° C for 15 minutes in a metal tray capable of sterilization at high temperature. The extracellular matrix thus extracted is stored at room temperature or higher, and the remaining bladder endothelium is collected separately, lyophilized, and disrupted with a base fiber powder.

Example  2. For skin moisturizing  Manufacture of natural complexes

An extracellular matrix extract, a 1% (w / v) aqueous solution of betaglucan, a 1% (w / v) aqueous solution of hyaluronic acid and a 1% (w / v) aqueous solution of silk peptide prepared by the method of Example 1 were prepared And deep ocean rock minerals were irradiated with gamma rays to prepare low molecular weight materials. The components were mixed in a weight ratio of 25: 10: 10: 10: 0.66.

Example  3. Preparation of natural composition composite composition

1 g of 50% ethanol was added to 100 g of dried and ground liquorice, persimmon, jaso, and green tea. The mixture was firstly extracted in a water bath at 50 ° C for 24 hours and then subjected to secondary extraction with 1 L of 50% ethanol. The extracted solution was filtered and concentrated in vacuo to recover about 25-30% of the extract.

Manufacturing example  One

The natural compound complex prepared in Examples 1 and 2 and the natural complex including the extracellular matrix and the mineral water were mixed to prepare a composition ratio as shown in Table 1 below.

order Raw material name Well clear content(%) One D.I Water Purified water 80.410 Natrosol 250HR Hydroxyethylcellulose 0.100 Allantoin Allantoin 0.900 2 D.I Water Purified water 3.000 Novaxan 80 Xanthan gum 0.100 3 Ethanol ethanol 10,000 Natural complexes containing extracellular matrix (ECM) Collagen, elastin, beta-glucan, sodium hyaluronate, silk amino acid 2.000 4 L-Menthol menthol 1,000 VE Acetate Tocopheryl acetate 1,000 5 EGY-820 Ethyl hexanediol, glyceryl caprylate 1.300 Corn oil Corn oil extract 0.200 Horseshoe / green leaf / green tea Hwaseongcho / Liliaceae / Green tea extract 0.200 Hong Kyung Chun Hong Kyung Chun Extract 0.200 Licorice extract Licorice extract 0.200 Vitamin B5 Pantothenic acid 0.200 Peppermint oil Peppermint oil 0.200 Mineral water (deep sea water) Seawater (300 times dilution) 0.200 Salicilic acid Salicylic acid 0.010 Triethanol amine (10% sol'n) Triethanolamine 0.500 Olivem 300 Olive oil phage-7 ester 2.000 C-20TX Setares-20 0.200 100

Test Example  One. ' Manufacturing example  1 < / RTI > of composition < RTI ID = 0.0 >

The number of times the mouse scratches the skin after itching with the itch inducer was measured in male ICR mice. Specifically, five ICR mice of each experimental group were each placed in a transparent acrylic cage, and left in the same experimental environment for 30 minutes for stability. 100 μl of 'Preparation Example 1' was dropped between both shoulders and rubbed so as to penetrate well, and Compound 48/80 (50 μg / site) dissolved in physiological saline was subcutaneously injected at a height between both shoulders such as a mouse And 30 minutes later, the number of scratching of the area where the itch inducing material was injected into the hindpaw was counted and evaluated. Conversely, 30 minutes after inducing the itching, the composite composition was dropped on the area, rubbed so as to penetrate well, and the number of scratches was measured.

As shown in FIG. 1, the number of times of the compound 48/80 control group (195 ± 21) was significantly increased compared to the number of scratches of the normal control group. However, when a composite composition containing 1% and 2% of extracellular matrix (ECM) was sprayed, the itching relief effect was significant in the itch induced model mice induced by Compound 48/80 (1%: 75 ± 12, 2 %: 72 ± 11).

Test Example  2. ' Manufacturing example  1 < / RTI >

In order to examine the effect of the 'composition of Preparation Example 1' on the inhibitory effect of mast cell degranulation, histamine release inhibitory effect was confirmed using peritoneal mast cells. Tyrode buffer B (137 mM NaCl, 5.3 mM glucose, 12 mM NaHCO ₃ , 2.7 mM KCl, 0.3 mM NaH ₂ PO ₄ , pH 7.4) was added to BALB / c mouse abdominal cavity and the abdomen was massaged for about 2 minutes The peritoneum was opened and Tyrode buffer solution B containing peritoneal cells was collected with a Pasteur pipette. The cells were centrifuged at 1500 rpm for 10 minutes to precipitate the cells. Typed buffer solution B was added to the precipitated cells, and the cells were untied by pipetting. Then, only mast cells were recovered by percoll density gradient method. Tyrosine buffer A (137 mM NaCl, 1.8 mM CaCl ₂ , 1.1 mM MgCl ₂ , 12 mM NaHCO ₃ , 2.7 mM KCl, 0.4 mM NaH ₂ PO ₄ , 5.6 mM glucose, pH 7.4) containing 0.1% Cells were seeded at 2 × 10 ⁵ cells, cultured at 37 ° C for 20 minutes, and the samples were treated for 20 minutes. The composition of Preparation Example 1 was treated in different amounts, and after 1 hour Compound 48/80 5 占 퐂 / ml was treated for 30 minutes. After 30 minutes, supernatant and cells were separated by centrifugation at 2000 rpm. Then, the supernatant sample thus obtained was quantitated by histamine ELISA kit.

As shown in FIG. 2, when treated with compound 48/80, the normal peritoneal mast cells were increased to 159 ± 20.2 ng / ml. When the compound composition was treated with 5 ~ 50 μl / ml, the free histamine was dose- , Respectively. The inhibition rate was about 51% and 67% at 25 and 50 ㎕ / ml, respectively.

Manufacturing example  2. ' Manufacturing example  1 < / RTI > Snow form  Confirmation of form injection

In order to increase the residual rate of companion animal hair, it is spray type spraying, and the product is made into a viscous spray which spreads like snow. Such a snow foam form was implemented using a foam generating container.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Of course it is possible. Accordingly, the scope of the present invention should not be construed as being limited to the above-described embodiments, but should be determined by equivalents to the appended claims, as well as the following claims.

Claims (7)

Natural complexes including extracellular matrix (ECM), beta glucan, silk peptide; And
A composition for controlling animal fecal matter comprising, as an active ingredient, a natural compound complex extract containing licorice,
The composition according to claim 1, wherein the extracellular matrix (ECM) is derived from a pig bladder endothelial tissue by decellularization with sodium hypochlorite and disinfection.
The composition according to claim 1, wherein the composition further comprises mineral water
[Claim 4] The composition according to claim 3, wherein the natural complex, the natural product complex extract and the mineral water are mixed in a weight ratio of 4: 1: 1
The composition according to claim 4, wherein the mixture comprises 2 to 15% by weight of the composition.
The composition according to claim 1, wherein the composition is for skin moisturizing or fur itching improvement
The composition according to claim 1, wherein the composition is prepared from a shampoo, a rinse, a conditioner, an essence, a lotion, a treatment, a cleaner and a moisturizer

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