KR20130008211A - Extracts isolated from animal bladders, constituents of the extracellular matrix that contains material that promotes intercellular signaling, and protein composition of the manufacturing process that includes cell regeneration - Google Patents

Abstract

PURPOSE: A cell regenerative functional composition containing a protein with an intercellular signal transduction promoting material is provided to regenerate and recover damaged cells. CONSTITUTION: A cell regenerative functional composition which regenerates cells of an injured animal contains 0.1-99 wt% of a protein as a medicine which is a constituent of ECM(extracellular matrix) containing intracellular signal transduction material(STPM) isolated from the bladder of an animal such as a pig, cow, sheep, deer, or goat. The protein is prepared by washing the bladder with surfactant and water, quickly freezing, primarily pulverizing, freeze-drying, secondarily pulverizing, and filtering. The protein is collagen containing elastin and chondroitin.

Description

Extractive isolated composition from animal bladders, constituents of the extracellular matrix that contains material that contains protein, a component of extracellular matrix, which contains intercellular signal transduction promoter isolated from animal bladder promotes intercellular signaling, and protein composition of the manufacturing process that includes cell regeneration}

The present invention provides a protein (collagen, elastin, chondroitin) which is a component of an extracellular matrix (hereinafter referred to as ECM) containing an intercellular signaling promoter (hereinafter referred to as STPM) isolated from an animal bladder and injects the protein into an injection, It relates to providing a cell regeneration functional composition comprising as an internal or external drug.

To regenerate cells damaged by abrasions, burns, surgery, ultraviolet rays, skin cells damaged by aging, and organ cells damaged by various diseases, cell regeneration material is extracted from plants and minerals to regenerate cells damaged by various causes. And it is widely used for the treatment and prevention, treatment, and improvement of aging symptoms.

However, the existing cell regeneration agent has a slight effect on abrasion, regeneration of damaged skin by surgery, but the effect of regeneration of cells damaged by burns, ultraviolet rays, aging skin is very small.

In addition, skin diseases caused by lack of collagen contained in cells include protein collagen essential oils extracted from skins of fish, cows, pigs, and plant materials, to prepare external preparations, and to absorb collagen contained in the external preparations into the skin. Many external skin compositions including collagen have been provided for regenerating damaged cells.

However, the collagen extracted from the skin and botanicals of the fish protein, cattle, pigs is very limited to absorb the collagen into the subcutaneous tissue because the skin does not open the cell membrane.

Thus, DMS or DMSO or IPM, which is a sulfur compound, is included in the external composition for skin to force collagen into the subcutaneous tissue.

However, collagen infiltrated collagen differently from the human body through the skin cell membrane does not sufficiently perform the role of collagen as compared to collagen produced by the cell itself.

The reason for this is to share information on the mechanisms for cell regeneration between and within cells. In other words, a signal material for information transmission is required.

Accordingly, the external preparation for skin regeneration of wounds containing only existing collagen has a very small effect on cell regeneration.

In cellular regeneration, intracellular signaling is generally performed by a signaling material, and hormones, neurotransmitters, and the like are typical signaling materials. When a signaling substance is released from one cell, the foreign substance acts on a receptor ion channel on the other cell membrane to transmit a signal.

When these signals are transmitted, intracellular signaling mechanisms are activated in the cells that receive them, resulting in a variety of reactions, such as the expression of specific genes in the nucleus and the production of specific proteins or the release of signaling substances.

All the phenomena that influence cell death, such as cell division or apoptosis, also occur according to signals. As such, intercellular signaling is an important physiological phenomenon that is involved in all life phenomena, and various diseases are caused when abnormalities occur in the body's signaling mechanism.

In the intercellular signaling mechanism, a substance that promotes a signaling substance is involved. In other words, if there is a lack of a substance that promotes signal transduction, many cells that make up a living organism have problems with signal trancluction, and physiological phenomena cannot exchange information with each other through intracellular or intracellular signal transduction processes. Control is impossible, and disease occurs in cells that do not have physiological control.

From ancient times, ancestors usually kept pigs, cows, sheep, goats, and deer's bladder dry in the shade, and when there were deep wounds on the skin, the bladder dried with a knife was sprayed and healed.

The inventors of the present invention, while studying the collagen contained in the skin of each type of living things, such as cattle, pigs, sheep, goats, deer bladder and fish, skin, most of the animal bladder is made of collagen, It is hard to damage even if it is put under pressure, and in the study of the components of bladder tissues of animals, it has been found that especially pigs have the most similar conditions to the human body. As a result of intensive research and analysis, it was found that a large amount of G protein that promotes intercellular signaling material was included, and the result of the study of the protein of the extracellular matrix as an E-cell cushion material led to the present invention.

The bladder of the pig is composed of collagen, the tissue of which is mostly protein, and the protein contains a large amount of a signal transduction facilitator for transmitting information for the smooth regeneration of cells between the cell and the surrounding environment.

In addition, the bladder of animals, including pigs, is a waste material that is disposed of in slaughterhouses, and its resources are so rich that it can produce economical intercellular signaling and its promoters.

Accordingly,

Accordingly, an object of the present invention is to provide a protein, which is a constituent of ECM containing intercellular signal transduction promoter, from a bladder of an animal, in particular a pig, without any change in its components.

In addition, the present invention provides a method of absorbing an active substance through a cell membrane using a cell membrane ion channel and absorbing it into a cell, and a use material thereof.

In addition, another object of the present invention is to provide a cell regeneration composition as an injection, an oral or external skin preparation including a protein containing a cell regeneration signaling promoting agent as a drug.

In order to achieve the above object, the present invention, the collagen containing the intercellular signal transduction promoter (hereinafter referred to as STPM) isolated from the bladder of the animal, including pigs, in the form of a powder or liquid in the cell regeneration agent composition of the animal It can be characterized by including 0.1-99% by weight of the cell regeneration active material.

The present invention is prepared by performing the following process.

1. Bladder collection of animals-washing-primary crushing-freezing-vacuum drying-secondary crushing-filtering-powder collection.

2. Bladder collection-washing-quenching and freezing-grinding-impregnating with alcohol-stirring-filtering-protein collection and drying-powder collection

3. a. Bladder collection-washing-quenching-grinding-fermenter input-sap and microbial input-fermentation-filtering-protein liquid collection.

b. Filtering after fermentation-protein collection drying-grinding-powder collection.

2 and 3 a. Sulfur or its compounds and derivatives thereof may be included depending on the intended use of the target in step b.

In addition, the sap of the tree may be used instead of water in the process of 3, and a detailed description thereof will be described below.

Extraction or STPM extraction of the protein containing the IM (intracellular messenger) from the bladder of the animal including the pig may be performed by performing a number of processes as follows.

For example, implementations of the following process may be provided.

1. Powder extraction process.

The powdering process is a drying process by any one of the drying process selected from thermal drying, cold drying.

In the extraction process by hot water, active ingredients other than collagen may be destroyed or altered.

In this process, the general purpose of quenching, then crushed to freeze vacuum drying process is performed.

50-300 mesh crushed fine particles of frozen bladder frozen at -15 to -35 degrees, finely formed, and then put into a vacuum chamber and heated to a temperature of 30 degrees Celsius to 70 degrees Celsius, immediately before being converted into water. Gasification to obtain a dried bladder crushed, and the dried material is pulverized with a second 300 mesh-1 nanomolecules by a pulverizer to prepare a high-purity protein containing the collected STPM.

By another method, the bladder of the animal, including the pig, can be lyophilized.

Animal bladder may be rapidly frozen by contact with liquid nitrogen, and frozen bladder may be extracted with protein powder from the animal bladder by the vacuum drying method.

2. Hot Water Extraction Process

The second powdering process is a powdering process by conventional hot water extraction.

Extracting collagen by the hydrothermal process may destroy or change the components of an active substance useful for cell regeneration other than collagen.

The washed animal's bladder was crushed into 1mm-15mm particles and impregnated in distilled water. The impregnated material was stirred and heated for 1 hour-24 hours at a temperature of 60-70 degrees Celsius, or 10 minutes-1 hours 30 minutes at 100 degrees Celsius or higher. In addition, collagen containing STPM can be isolated from the bladder cells of the animal. In the above temperature, the STPM may be destroyed above 70 degrees Celsius.

3. Alcohol extraction process.

The washed and shredded animal's bladder lysate was impregnated with about 70% alcohol for 3-30 days to filter out the regenerated collagen from the animal's bladder, and the alcohol containing residual STPM was heated to 30 degrees Celsius to 70 degrees Celsius. Evaporate to extract STPM.

In the alcohol extraction step, a protein as an IM or a protein as an ECM can be obtained from the bladder of an animal including a pig.

4. fermentation extraction;

The bladder lysate of the animal, which is washed by the washing process and crushed by the crushing process, is put into a fermenter, and the fermentation broth of fermented STPM-containing collagen is obtained by fermenting the sap and microorganisms.

The fermentation method is a liquid fermentation method,

The fermentation method and the microorganism used may be selected from a general liquid fermentation method and a general purpose microorganism.

The hydrothermal extract can produce collagen (including elastin and chondroitin), and can produce high quality STPM by the alcohol extraction and fermentation extraction. Accordingly, by mixing the hot water extract and the alcohol extract, it is possible to specify a feature of obtaining a protein having a high quality IM or STPM mixed therein.

In addition, a process of drying and pulverizing the washed bladder of the animal by using a low temperature warm air drying method of 30 degrees Celsius-70 degrees Celsius may be used.

In addition, the sap of the tree can be used to replace the water in the fermentation extraction process.

The sap of the tree contains abundant amino acids necessary for the human body, and calcium among these amino acids works with other sap components to promote the production of filaggrin and / or propyllagrin, and to promote the production of inblue cleansers. There is a function of producing and cell regeneration.

STPM promotes the smooth signal transmission between cells and the surrounding environment of the filaggrin and / or propyllagrin and inbuluclin, which plays a large role in regenerating cellular tissues, thereby repairing wounds. The time is shortened.

Sulfur may be added to the bladder fermentation process in animals, including pigs.

The use of tree sap and sulfur in fermentation of bladder including pigs is involved in fermentation of bladder with sulfur amino acids Methionin, cysteine and cystine by working with amino acids contained in tree sap.

In addition, one of the reasons for mixing tree sap and sulfur in a composition comprising ECM including STPM, which is a bladder extract of an animal including pigs, as a cell regenerative agent, is an amino acid which is a protein contained in sap of tree. Sulfur amino acids such as cysteine, cittin, and methionine, which act as sulfur, act as keratin protein structure in the epidermal layer of the epidermis, purifying and detoxifying harmful substances that accumulate in cells and interfere with cell regeneration. Preventing water loss increases STPM's cell regeneration function.

In addition, another reason for including tree sap and sulfur in the collagen composition, which is a bladder extract of an animal including pigs, is one of the roles of sulfur, a triple spiral structure as a protein for maintaining elasticity of regenerated cells. Amino acids constituting collagenase enzymes directly involved in collagen molecules, which are proteins containing STPM extracted from bladder cells of animals, including pigs, which aggregate with various tissues, that is, adhesives As a holding role, including the regenerated cells to enhance the function of maintaining the elasticity of the cells.

Accordingly, the tree sap and sulfur or a compound or a derivative thereof are added to a protein extracted from an animal bladder including a pig, so that the composition may be formed.

Another reason why tree sap is included in the composition of collagen comprising STPM extracted from an animal bladder comprising a pig is that tree sap is an anionized water containing substances essential for body cells. It penetrates into the cell as ionized water and uses the ion channel to open the cell membrane without rejection.

At this time, ECM or STPM as a signaling system protein containing STPM and other functional active ingredients required for cell regeneration are rapidly infiltrated into the cells like sap of trees.

The sap of the tree refers to the liquid raised to the upper part between the epidermis and the wood of the tree, and the sap may be leaked by cleaning the epidermis and the wood.

Tree sap is nontoxic and can be used by spilling sap.

Accordingly, the characteristics of using the sap of the tree as an absorbent for penetrating into the cell the composition containing the protein (collagen, elastin, cordroycin) containing the ECM and STPM extracted from the bladder of the animal including the pig can be specified. have.

In order to improve the function of the protein containing the ECM, STPM extracted from the bladder of the animal, including pigs, among the substances having a cell or cell regeneration function of plant or animal and mineral in the range that does not affect the degeneration and degradation of the protein One or more selected cell regeneration materials may be included to form a cell regeneration agent composition.

The composition of the present invention may be formulated in powder form, cream form, liquid form, or the like,

For example,

1. Powder form composition may be composed of 50-95% by weight of protein containing ECM, STPM obtained by the powder process, 5.0-50% by weight of sulfur or a compound thereof.

2. The composition in the form of ointment or cream may comprise 1.0-10% by weight of collagen containing STPM, and 0.01-3.0% by weight of sulfur or its compounds or derivatives thereof. And 0.01-3.0% by weight of alum, 0.05-3.0% by weight of rosin or calendula, and 50-85% by weight of tree sap to enhance the effects of cell production. 0.01-0.1% by weight of calcium oxide, 0.5-1.0% by weight of seaweed may be further included.

4. The liquid composition may be composed of 1-10% by weight of the ECM construct containing STPM protein, 0.01-3.0% sulfur, 50-95% tree sap. In addition, it may be configured to include 0.01-1% by weight of alum, 0.05-1% by weight of rosin or calendula, 0.5-1.0% by weight of seaweed extract, 0.01-0.1% by weight of calcium oxide solution.

In the composition of the present invention, homocysteine may be generated in the metabolism of intracellular sulfur amino acid, and homocysteine functions to indiscriminately attack and destroy blood vessels.

At this time, B6 converts homocysteine to cysteine, and B12 reduces homocysteine to methionine.

According to the functional test of the composition of the present invention, there was no homocysteine in the metabolism of sulfur amino acid in the composition of the present invention, but vitamin B6 (pyridokicin) and vitamin B12 (cyanocobalamin) were further added to prevent this from occurring. You may add 0.001-0.1 weight%, respectively.

The bladder of the animal is a bladder of at least one animal selected from pigs, cows, sheep, goats, and deer, and the choice of the bladder of pigs is a priority.

In the present invention, materials added to a composition comprising a protein which is a construct of an ECM including STPM will be described.

Sap

The type of sap used in the present invention is not limited to the sap collected from any particular tree, and it is a sap that is not toxic, and the effect lowering effect due to the chemical reaction between the components of the sap and the components of the composition. If there is no sap of the tree can be used to mix more than one kind.

Trees that can shed a large amount of sap by drilling the skin and wood parts of trees are as follows.

At least one tree of the Betaceae, at least one tree of the Aceracea, at least one tree of the Bambusoideae, at least one of the vines, and at least one of the Cornaceae. At least one tree in the Juglandaceae, at least one tree in the Oleaceae. It may be one of the tree species of the Tiliaceae. Or Pistachia letiscus native to a subtropical region.

For example,

Trees that can use sap in the Birchaceae family include Birtaceae, Betula platyphylla, Betula ermanii, Betula davurica, and Betula schmidtii Alder tree. , Carpinus, Betula costata, and more than one kind of tree sap can be selected and used.

Trees that can use sap among maple trees are maple tree, cypress, maple, mountain elm, and god tree, and sap trees among prunus trees are royal trees, and sap trees among dogwood trees are dogwood and cornus Trees that can be used in the sap of the sputum tree are sputum, hawthorn tree, and ash tree in the ash family.

A vine family that can cut the stems and give a large amount of sap to sunrise is the stalk tree (Actinidiaceae). Grapes (Annonaceae). Nova vines and trees (Celastraceae). Sap of one or more trees selected from ivy and trees (Lardizabalaceae) can be used.

The nature and components of the tree and its sap used for the present invention will be described.

The sap in the present invention is not limited to the sap of the tree described below.

For example, there are about 200 kinds of maple trees in two families, but among them, old cedar, mountain elm, and maple. You can use sap taken from more than one type of tree such as maple.

For example, the physicochemical properties and food components of mixed sap of buckthorn tree, alder tree, beech, birch, and cypress sap collected from Cheorwon-gun, Gangwon-do, Korea are as follows.

In the mixed sap of the maple family, the water content is 97.6, the solid content is 2.3%, the crude protein is 0.08%, the ash content is 0.03%, the pH is in the range of 6.4, and the mixed sap specific gravity is 1.007. Nucleic acid-related substances in the sap are CMP, UMP, IMP, GMP, AMP and hypoxanthine, totaling 0.1um / ml. The main free sugars of maple trees were sucrose 23g / L, fructose 0.3g / L and glucose 0.5g / L.

A total of seven minerals are contained, and their contents are as follows. Calcium 112mg / L, Iron 0.1mg / L, Copper 0.01mg / L, Zinc 0.24mg / L, Potassium 65mg / L, Magnesium 8.2mg / L, Sodium 5.14mg / L. The constituent amino acids in the sap are 10.3mg% Taurine, 0.5mg% Serine, 1.8mg% Glutamic acid, trace Aspartic acid, trace Thr, trace Gly, trace Ala, Cys trace, Val 0.1mg%, Met trace, Ile 0.1mg%, Leu trace, Tyr trace, Phe 0.1 mg percent, Lys 0.3 mg percent, His 0.1 mg percent, Arg trace, Pro trace. (Trace: 0.1mg% or less)

Another example of the birch family is described as follows.

Birch family consists of 7 genera and 100 species, including birch, manchurian birch, birch, water birch, birches, giantwood, seobari, alder, and so on. The physicochemical properties and food components of the collected birch and mixed sap are as follows.

Sugar content is 0.1 ° -1.8 ° Brix, crude protein 0.1% -0.3%, mineral is calcium 50.5mg-206.0mg / L, potassium 57mg-181.9mg / L, magnesium 6.6mg-26mg / L, sodium 0.1mg-23.2mg / L, iron 0.0mg / L-0.1mg / L. The content of free sugar is Glucose 2.5-42.1g / L, Fructose 4.5-36.9g / L and Sucrose 0.2-5.7g / L.

Amino acids were 0.2 mg% -3.2mg% Glutamic acid, 0.3-1.2 Tau. Asp 0.2-0.6. Thr 0.1-1.9. Ser 0.1-0.7. Gly0.1-0.5. Ala 0.1-2.9. Cys 0.1-0.8. Val 0.1-0.7. Met 0.1-0.3. Ile 0.1-0.6. Leu 0.1-0.6. Tyr 0.1-0.2. Phe0.1-0.6. Lys 0.4-0.6. His 0.3-0.6. Arg 0.1-0.4. Pro 0.0-0.1 with an average of 2.3-14.2 m /%.

Nucleic acid related material is CMP 0.2-0.3 μ mol / ml. UMP 0.5-1.2.

Another tree is Bamboo, which has about 1,250 genera. Among them, cotton, blind and royal, the composition is slightly different depending on the species.

Physical and chemical properties and food analysis of bamboo mixed sap collected from Damyang-gun, Jeonnam, Korea are as follows.

Sugar 0.5-0.8 Brix, Crude Protein 0.07-0.18, Minerals Calcium 242.0mg-422.1mg / L, Potassium 793.8mg-2504mg / L, Magnesium 72.6mg-165.9mg / L, Sodium 37.4mg-46.2mg / L, Iron 0.3 . The content of free sugar is Glucose 1.8-3.4g / L, Fructose 1.4-2.9g / L and Sucrose 1.6-2.5g / L.

Amino acid is Glutamic acid 0.2mg% -3.5mg%, Tau 0.0-0.2. Asp 0.1-2.9. Thr 0.6-2.0. Ser 1.3-3.6. Gly 0.6-1.4. Ala 0.3-1.8. Cys 0.1-0.3. Val 0.4-1.5. Met 0.1-0.4. Ile 0.2-1.2. Leu 0.2-2.2. Tyr 0.1-4.5. Phe 0.2-1.2. Lys 1.0.-2.3. His 0.3-0.6. Arg 0.3-1.3. Pro 0.5-1.8, average 7.6-29.2 m /%. Nucleic acid related material is CMP 0.0-0.1 μmol / ml.

There are about 300 species of vines and tarasiaceae in 4 genera. Among them, there is a worm, a dog worm, a rat worm, a sheep worm and the like.

The physical, chemical, and food analysis of the mixed sap of Caesarena and T. aerugia in Cheorwon-gun, Gangwon-do, Korea, are as follows.

Sugar 0.0-0.1 Brix, crude protein 0.07, minerals calcium 223.4mg / L, potassium 857.5mg / L, magnesium 69.9mg / L, sodium 15.2mg / L, iron 0.2mg / L. The content of free sugar is 0.3 g / L Glucose and 0.5 g / L Fructose.

Amino acid was 15.1 mg% of Glutamic acid, Tau 0.0-0.0. Asp 0.2. Thr 0.6. Ser 0.5. Gly 0.6. Ala 2.6. Cys 0.2. Val 0.9. Met 0.2. Ile 0.5. Leu 0.8. Tyr 5.7. Phe0.7. Lys 1.3. His 0.0. Arg0.1. Pro 0.4 averaged 30.4 m /%. Nucleic acid related material is CMP 0.0-0.1 μmol / ml.

As can be seen from the above components of the tree sap is similar.

The description of alum is as follows.

It is called alum. It has the general formula of MIA1 (SO4) 2 * 12H2O or MI2SO4 * Al2 (SO4) 3 * 24H2O. It is also called potassium alum Kali (SO4) 2 · 12H2O, ammonium alum (Cermigite) (NH4) Al (SO4) 2 · 12H2O, etc., depending on the monovalent metal ions contained. .

In addition, MIMIII (SO4) 2 · 12H2O (MIII = Ti, V, Cr, Mn, Fe, Co, Rn, Ir, Ga, In, etc.) in which other trivalent metal ions are substituted in place of aluminum is also alum. According to each metal ion, for example, ammonium-iron alum, rubidium-cobalt alum, and the like. At this time, potassium and aluminum are omitted, for example, KFe (SO4) 2.12H2O may be referred to as iron alum, and KAl (SO4) 2.12H2O may be called only alum. Used in medicine as astringent.

When alum is mixed with sulfur, it promotes cell activity, softens the skin, and enhances the bactericidal effect, the treatment effect of boil and boil.

Rosin (Resin, rosin).

Rosin is also called Songji (松脂). Precipitate a white solid from resinous acid. It is soluble in ethanol, chloroform and acetic acid but insoluble in water, and rosin remains after distillation of water vapor to obtain tevin oil. Resin is composed of 70-75% of resin (rosin), 18-22% of essential oil (terebin oil), and 5-7% of water and other impurities, among which 60-65% of lactic acid is levopimaric acid. It consists of neoabietin acid, etc. Sterilization, sebum secretion control, moisturizing, anti-arregi, promotes skin metabolism, antioxidant activity.

Shell calcium oxide

Calcium oxide obtained by calcining the shell at high temperature with a strong alkaline pH of 13-14. However, even though the pH is strong alkali, it is not corrosive at all. Prolonged contact with skin does not cause any irritation. Cell regeneration, bactericidal action.

Sulfur or compounds thereof.

Sulfur is as described above and adding the effect, there is an effect of whitening, skin flexibility, blemishes, skin elasticity, exfoliation of the skin.

Sulfur compounds are DMS, DMSO, DMSO2 and one of the compounds is selected and included in the composition.

The choice of sulfur compounds is DMSO2 because of dimethylsulfonymethane, which is a side effect that can occur in sulfur or other sulfur compounds and is a very stable material with no toxicity or odor.

Haedyocho

It is bone of cuttlefish, a kind of sea fish, and dried Chinese medicine name. Cell regeneration function and bactericidal function.

The use of the composition of the present invention will be described below.

The composition in the form of powder can be injected into the human body through the oral cavity, the function can be used for the regeneration of damaged cells in the body, and can be used for cell regeneration of severe burns or severely damaged wounds of more than 3 degrees.

In addition, ointments, creams, liquid forms of the composition can be used as an external preparation for skin regeneration of damaged cells of the skin, the prevention, improvement and prevention of aging wrinkles.

The following materials may be further added for improving the functionality and formulation of the composition.

For example:

Vegetable wild grass that can extract a functional material in the present invention. The neck is as follows: Sanyacho. At least one of the roots, stems, leaves, and fruits of the neck may be used.

San-yacho refers to the type of grass that grows in the mountains and fields, and neck refers to the type of tree.

In the following, it is described to include a kind of tree in Sanyacho.

Depending on the efficacy and use of the material, the same name as the tree species described below may be described in duplicate.

cabbage. radish. Lettuce. Wormwood. sweet potato. potato. pumpkin. Cheonma. mint. Lavender. Trilobite vinegar. rose. yarrow. Darae. Seokchangpo. ringworm. Wasabi. Guava. Hot pepper, shovel. Korean ginseng vine. Kalanchoe Fruit. Bokbunja. Sewao. Buddha hand. Cold grass. Noisy. Blooming tree. mistletoe. Ginseng. Non-repair. Sky. Fast. Ho Jang Geun. Gallweed. Sawing bread. yam. Country of origin. hepatica. purslane. dandelion. Hawthorn. Seaweed. Three hundred seconds. Maple leaves. Morning Star Flower. Body herb. camphor tree. Pine tree. Bellflower. New Year. Soothe. swimming. Forget-me-not. Birch tree. Tenza, acacia (flowers). Buling. Seokgok. Chicken cobs. Injin mugwort. Turtle tail. rowan. 꽃 (flower). Ginseng. Stone top. It's round. Write. Gomari. File line. Brier. Angelica. Inner bird. Spinach Sprouts. celandine. Nose. Ash water. Crush vines. Celestial. Sulfa lang. Scab flower. primrose. Morel cabbage. Lagerstroemia. Juniper. Todeok. It's baby. cottonweed. Eoseongcho. Pear cancer tea. Azaleas. Aftertaste. cranesbill. Fat. Sunny flower. sulphate of soda. Ginger tree. olive. Noni. Blue rice vine. Wild ginseng. motherwort. White hair root. Elegy. Bat Sprouts. Yeok Tree. Gardenia (flowers). Camphor. Zinnia. knotgrass. Thistle. Bat tree. Nasturtium. Paulownia. Arrowwood Bark. Saenghwanghwang. Silkworm grass. water chestnut. Here. Beetle herb. Oak. High light tree flower. Reed. Shellfish. Matari. Lotus flower (leaves). Scruffy. Honeysuckle. Perilla leaves. Cortex. Tired. convolvulus. Cucumber. Hyangbuja. New beans. Albizia julibrissin. Sesame Tree Fruit. Kowloon tree. Income. Daughter-in-law. Nose. Scoop. Graft flower. Tofu (leaves). pasqueflower. Water and water. Umbrella herbs. Campanula. Privet. Chinese quince. Gratefully. Clogs. Water seal. Self-operated. Foxtail. plum. Plum blossoms. Madly. Buttercup. Jang-chae. Repair odor. Arms & Trees. Dumbberry fruit. Gosam. Copper violet. Singa. Castor elderflower. Ripples. Michaelmas daisy. Seaweed smell. Jovan. Worms. Rhododendron. Evening primrose. Rainy tree. Camellia. Gujeolcho. Individual flower. Park Jugary. Little Pheasant's Leg. Aeginari. Wolfberry. Pheasant's amaranth. Sea wind. Pearweed incense. Wrinkled leaves. Assurance. boxwood. Wangsam. Presser foot. Spinal sprouts. Count. Qin. Pole Herb. Thick wood. B. Heat up. Vast herbs. magnolia. Snake Strawberry. Plantain. It's kinda cool. Hazel. Yeonsam. Docomari. Hoebab. Burnout. Straw Sprouts. Roots. Oak tree. Goldenwood. Herbs. Golden Rancho. Flea herb. Loquat leaf. Chazuki. Virgin body. Gucci mulberry. Loincloth. Round rock brush. Giraffe. chickweed. A rumor. Tiered Remnants. Novak. Macmundong. In the middle. Amaranth. Booze. Large dogball ball. Tree berry. Rock hands. Fixed car. Hong Kyungcheon. Flowers. purslane. Magpie. Large repair. Cedar. Rock brush. Marigold. Casualties. Hairy ears. Hairy manure. Branch. plum. Graft alley. Cheonnyeoncho (College Cactus Cactus). Centennial. Paprika, fixed car. Schisandra. peach. Jasmine. geranium. Sorghum. rice. article. Big head. red bean. barley. wheat. corn. green gram. Bon Bridge. Rice bran. plum. Lemongrass,

Trees from which cell regeneration functional extracts can be obtained are, for example, as follows.

Depending on the efficacy and the use of the material, the same name as that described above may be described in duplicate.

Red bean tree. Nettle. Noni tree. Eggplant tree. Persimmon tree. Bat tree. sumac. Oleander. Ratberry. Gourd trees. Barberry. Chichi. boxwood. A base flower. Pine wood. Hibiscus tree. Cassia. Single widower flower. Plum duck. Chrysanthemum. Crassulaceae. Cycads. Oyster tree. Pit bush. Shiromi Tree. Rhododendron. Great Jack. kind of oak. Paddy. Evergreen. Horse chestnut. Water and water. Irises. Lamiaceae. camphor tree. Beanstalk. Lily tree. Horsewood. Mistletoe tree. Buddha flower tree. Magnolia tree. Mallow. Mulberry. Bangui. Noble tree. The capital. Nissa. Needle flower tree. Peony tree. Palm. Paper mulberry, pine. Black pepper. Money tree. Plane tree. Han Han Song. Roundwood. Pomegranate. Buttercup. Rosewood. Madly. Tree without leaves. Guilty ears. Schisandra chinensis. figwort. Pine tree. Chilli. Stink bug tree. Satellites. Larch. tea. Verbena. Elm. Cypress. Plum tree. Birch. Ginkgo. Cedar tree. fist. Persimmon tree. Buckthorn. Fortune telling. Lime tree. mulberry tree. Beech. Bud tree. Bamboo, Jacaranda, Birch, Skeleton, Maple, Bamboo, Sputum, Dogwood, Blooming Tree, Cedar, etc. Among the above-mentioned trees, more than one kind selected from the root, stem, leaf, bark, fruit that.

And juices extracted by pressing at least two kinds of stems, bark, roots, leaves, fruits of at least one tree selected from the group of the genus can be used.

In addition, the present invention can be added by functionally classifying and selecting wild grass and trees according to the condition of the skin as follows. Next, the same name as the above-mentioned wild grass or tree may be described as a plurality of uses as extracts of wild grass.

Vegetable antipruritic

Morel cabbage extract, Seokchangpo extract, Eoseongcho extract, nasturtium extract, casualty extract, oxtail extract, tanza extract and the like.

Vegetable antioxidants

Cinnamon extract, Geraniumthunbergii extract, Mountain lion extract, Peony extract, Ginkgo biloba extract, Scutellaria baicalensis Georgi (Golden) extract, Rosae rugosae Flos. Lactobacillus, Gypsum Extract, Tormentilla Extract, Grape Extract, Chacha (Yasha) Extract, Saxifragastolonifera Extract, Rosemary Extract, Tea Extract (Oolong Tea) , Black tea, green tea, etc.), apricot extract, cherry extract, plum extract, peach extract, tangerine extract, rose extract, plum extract, lemongrass extract, yabasanta extract, miracle berry extract and the like.

Vegetable anti-inflammatory

Aloe extract, fresh vinegar extract, nettle extract, nettle extract, Injin mugwort extract, turmeric extract, bark extract, HyperiCum erectum extract, chamomile (kamille) extract, Comfrey extract, Lonicerajaponica extract, watercress extract, salvia extract, Sanguisorba officinalis (fatty oil) extract, jasminoide extract, birch extract, graft tree extract, vulgaris extract, unaffected extract, eucalyptus Extract, mugwort extract, lotus leaf extract, Sasa veitchii extract, zinc oxide and the like.

Plant cell inactivators

Asparagus extract, soybean extract, jujube (大 조) extract, garlic extract, ginseng extract, beech extract, royal jelly, yeast extract, microbial fermentation metabolite, ganoderma lucidum extract.

Vegetable moisturizer

Almond Extract, Avocado Extract, Althea Extract, Aloe Extract, Mallow Extract, Ononis Extract, Oat Extract, Glabridin, Glabrene, Liquiritin, Yissori Curietin and licorice extract containing it, Quince seed extract, Red ginseng extract, Gardenia Jasminoides extract, Grapefruit extract, Watercress extract, Gentiana extract, (Geranium thunbergii) Extract, Burdock Extract, Wheat Extract, Sessin Extract, Cactus Extract, Saponaria officinalis Extract, Sansa Extract, Gemini Extract, Spirea japonica Extract, Ginger Extract, Mallow Extract, Mulberry Extract, Thyme Extract, Cordyceps Sinensis Extract, Cordyceps Sinensis Extract, Peppermint Extract, Yul Radish Extract, Hamamelis Extract Water, rosewood extract, cypress extract, Tussilagofarfara extract, grape extract, prune extract, scrubber extract, linden extract, hop extract, pine extract, quince extract, Marronnier Extract, Melissa Extract, Centaurea cyanus Extract, Lily Extract, Lime Extract, Lavender Extract, Apple Extract, Rice and Rice Bran Extract, Black Currant Extract, Bistortamajor Extract, Brier (Rosamultiflora) (vine brier) extract, prickly pear extract.

Seaweeds of the above-mentioned plants of the present invention may be one or more kinds of seaweeds selected from the following.

It is an extract of green algae, red seaweeds, brown algae, southern algae and axle algae.

The above-mentioned animal substances of the present invention are as follows.

Earthworms, lizards, snakes, seahorses, ants, centipedes, scorpions, hens, bonbons, turtles, growing up, cattle fences, sojira, small groups, cattle shells, pigs, pig fences, pig shells, antlers. Animal placenta. mantis. Fowler aphid bumps. Sea cucumber. squirrel. silkworm. Gomdam. Bracket. Animal placenta. It is water quality.

Cholesterol may be added to the composition of the present invention, and the types thereof are as follows.

Eggplant mushrooms. Liver mushrooms. Fresh mushrooms. Hazelnut mushroom. Egg mushrooms. Tiled mushrooms. Blossom mushroom. Oriole Mushroom. Crate mushroom. Oyster mushroom. Twill mushrooms. Chicken thigh mushrooms. Duck leg mushrooms. Horse mushroom. Wheat mushrooms. Giant mushroom. Mushrooms. Horseshoe mushrooms. Thirsty mushrooms. Shiitake mushrooms. Situation mushroom. Chestnut mushrooms. Pung fu mushroom. Mulberry Mushroom. Pine frequent stool mushroom. Cucumber flower mushroom. Matsutake. Mori mushroom. Fingering mushrooms. Shellfish Mushroom. Pine mushrooms. Shiitake mushrooms. Oak mushrooms. Ganoderma lucidum mushroom. Hin chimney mushrooms. Cherry mushrooms. Silk mushrooms. Drunken mushrooms. Private kitchen mushroom. Hackberry Mushroom. Blackcurrant. Cordyceps mushrooms. Tibetan mushrooms. Chaga is a mushroom.

The extracts may be hot water extraction, compression extraction, alcohol extraction, extract by fermentation.

Fatty acid, moisturizers, fatty acids, minerals, alcohols, polyhydric alcohols, emulsifier waxes, esters, vitamins, α-hydroxy acids, ultraviolet absorbers or blockers, whitening agents, amino acids, etc. Gums, sugars or water-soluble high molecular compounds, organic powders, inorganic pigments, active oxygen scavengers, turnover accelerators, cell activators, surfactants, chirosin activators, melanin pigment reduction or degradation agents, Astringents, lipid peroxidation inhibitors, anti-inflammatory agents, elastase inhibitors, anti-androgens, blood stimulators, antioxidants, metabolic agents, keratin-soluble agents, cell-activators, alcohols, flavors, Fungicides, ph-adjusting agents may be additionally included in the composition.

Oil in the composition of the skin cosmetic composition may include one or more kinds of fats and oils selected from the following fats and oils.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, isopropyl palmitate, ethyl stearate, octyl palmitate, isotear stearate, butyl stearate, ethyl linoleate, isopropyl isopropyl, ethyl oleate, isocetyl triglyceride, tri2-ethylhexanoate, 2 ethyl hexane cetyl, isopropyl myristin, butyl myristic acid, isostyl acid isostearyl, isostearyl palmitate, octyldodedecyl, isocetyl acid isocetyl neopentanoate, isoalkyl, tri (capryl Caprylic acid) Glyceryl, tri2 ethylhexanoic acid trimethylol propane, diethyl sebacinate, diisopropyl adipic acid, trimethylolpropane triisostearate, penta erythritol tetraethyl hexanoate , Decyl laurate, hexyl laurate, mysterylate decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, cetyl ricinoleate, isolaurate Stearyl, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate Stearyl, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid) propylene glycol, neopentyl glycol dicapric acid Propylene glycol dicaprylate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tritridecyl acid, glyceryl triisopalmitate, triisostearic acid, glyceryl, octyl dodecyl octane, isocyanate Tearyl, octyl isononate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, octyldecyl isostearate, poly Lyserin Oleic Acid Ester, Polyglycerin Isostearic Acid Ester, Triisocetyl Citrate, Triisoalkyl Citrate, Triisoctyl Citrate, Lauryl Lactic Acid, Myristyl Lactic Acid, Cetyl Lactate, Octyl Decyl Lactate, Triethyl Citrate, Acetyl Triethyl Citrate Acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearic acid, diethyl 2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, dioctyl sebacate, stearic acid Acid cholesteryl, isostearic acid cholesterol, hydroxystearate cholesterol, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid phyteryl, oleic acid pitsteryl, 12 stearoyl hydroxy Examples of isocetyl stearate, 12 stealoyl hydroxy stearic acid stearyl, 12 stealoyl hydroxy stearic acid isostearyl It may be based, such as a hotel.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, Alkyl modified silicone oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable fats and oils include pus oil, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, camellia oil, castor oil, sunflower Oil, grape seed oil, cottonseed oil, palm oil, squaraine, cucunut nut oil, wheat germ oil, rice germ oil, shea butter, borage, chamomile oil, carrot oil, walnut colostrum oil, macadamia nut oil, meadow home oil, Animal or plant fats and oils such as egg yolk oil, tallow oil, horse oil, mink oil, orange rape oil, jojoba oil, candder wax, perilla oil, safflower oil, carnava wax, liquid lanolin, fish oil and hardened castor oil. .

Addition of humectants .

Moisturizer may include one or more of the following moisturizers.

Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or salts thereof, polyethylene glycol, chondroitin sulfate or salts thereof, water soluble kitchen or derivatives or chitosan derivatives, pyridone carboxylic acid or salts thereof, sodium lactate, minisasa Nishiki Ekisu, Naphruid bacteria metabolite, natto extract extract, loofah water, shirakaba (birch) or pine extract.

Addition of fatty acids.

One or more of the following fatty acids may be included.

Natural such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, polyunsaturated fatty acid, 12-hydroxy stearic acid, undecylenic acid, fee for use, lanolin fatty acid And synthetic fatty acids such as fatty acid, isononanoic acid, caproic acid, 2-ethyl butanoic acid, isopentanoic acid, 2-methylphenic acid, 2-ethyl hexanoic acid and isopentanoic acid.

Addition of minerals.

At least one selected from the following minerals can be added.

Liquid paraffin, petrolatum, paraffin, ozokeride, ceresin, microcrystalline wax, germanium, illite and the like.

Addition of alcohols.

One or more of the following alcohols may be added.

Natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, cetyl, stearyl alcohol, nile alcohol, lanolin alcohol, cholesterol, pitsteol, phenoxyethanol, 2-hexysirdecanor, ISO stearyl Synthetic alcohol, such as alcohol and 2 oak cyl decanol, etc. are mentioned.

Addition of polyhydric alcohols.

Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, multi, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide , Propylene glycol, polypropylene glycol, 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, trays, arabitol, xylitol, ribitor, garacchitor, sorbitol, mannitol, lactcitor, etc. Can be mentioned.

Emulsifier Wax (low) added

Meat, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montana wax, cerac wax, rice wax, emulsion wax. You can add more than one kind.

Ester addition

Myristin isopropyl, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristyl, nile oleate, diyl oleate, octylic acid octyl dodecyl, dimethyl octanoic acid disil, lactic acid cetyl, lactic acid myristyl , Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol monostearate and the like can be selected and added.

Add vitamins.

Vitamin A Group: Retinol, Recinar (Vitamin A1), Dehydroregina (Vitamin A2), Carotene, Lycopene (Provitamin A), Vitamin B Group: Thiamine Hydrochloride, Thiamine Lactate (Vitamin B1), Ribofurabine ( Vitamin B2), leaf acid, nicotinic acid, pantothenic acid, biotin, corin, inositol, vitamin C group: vitamin C acid or its derivatives, vitamin D group: ergocarciferol (vitamin D2), chorecar Cipherol (vitamin D3), geohydrotachisterol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: pitanzion (vitamin K1), menakinone (vitamin K2), menazion ( Vitamin K3), menazio (vitamin K4), and other essential fatty acids (vitamin F), carnitine, ferraic acid, ν-orizanol, orotic acid, vitamin P (rutin, erythrocirine, hesperidin ), You can add more than one kind of vitamin U.

α-hydroxy acid addition

One or more of glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid may be selected and added.

UV absorption or blocker addition

Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium , Geohydrocysimethoxy benzophenone, geohydrocysimethoxy benzophenone suronic acid, 2,4-zihydrokisi benzophenone, tetrahydrokisi benzophenone, etc.), paraamino benzoic acid derivative (paraamino benzoic acid, para) Ethyl amino benzoate, Glyceryl paraamino benzoic acid, Amyl benzoic acid benzoate, Paramethyl amino amino octyl benzoate, etc. Paramethic acid cinnamic acid 2-ethoxy ethyl, paramethic acid sodium cinnamic acid, paramethic acid potassium cinnamic acid, zipparamethic acid cinnamon products-2 ethylhexanoic acid glyceryl), salicylic acid derivatives (octyl salicylate, salicylic acid) , Satritylate homomentyl, satritylic acid propyrene glycol, ethylene glycol salicylate, salicylic acid myristyl, methyl salicylate), enthranilic acid derivatives (methyl entranilate), urokanoic acid derivatives (urocanoic acid, urokanine) Ethyl acid), bear phosphorus derivatives, amino acid compounds, benzotriazor derivatives, tetrazo derivatives, imidazorine derivatives, filimizine derivatives, dioxane derivatives, cardioid derivatives, franc derivatives, pyrone derivatives, nucleic acid derivatives, aran Open derivatives, nicotinic acid derivatives, vitamin B6 derivatives, unberyferon, escries, cinnamic acid benzyl, cynocitasse, okisibenzone, ziokibenzon, octabenzone, thrisobenzone, benzoresorcinolone, arbutin One or more substances selected from azulene, siconin, bicarin, bicarrain, berberine, neoheliopan, escarol, zinc oxide, talc, kaolin and the like may be added.

Addition of whitening agents

Paraamino benzoic acid derivatives, salicylic acid derivatives, enthranilic acid derivatives, bear phosphorus derivatives, amino acid compounds, benzotriazor derivatives, tetrazor derivatives, imidazorine derivatives, filimidine derivatives, dioxane derivatives, cardiac derivatives, franc derivatives, pyrones Derivatives, nucleic acid derivatives, aranthine derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate ester magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, koji acid or derivatives thereof, Okishibenzone, benzo It may be added by selecting at least one of phenone, arbutin, iaazene, siconin, bicarin, bicarin, berberine, placental extract, errag acid, lecinonor and the like.

Amino acid addition

Varine, leucine, isoleucine, threonine, methionine, phenyl alanine, lyphthhan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystin, chirosine, pro-phosphine, hydroxyproin, aspartic acid, glutamic acid, hydrin Loxy lysine, arginine, ornithine, histidine and amino acid derivatives such as lactate, phosphate, nitrate, citrate, or pyridonecarboxylic acid. It can be added by selecting more than one kind.

Adding gum, sugars or water soluble high molecular compounds.

gum arabic, benzoin bear, sweet margot, gayak, irish moss, karaya bear, tragant bear, carob gum, queen seed, agar, cold phosphorus, lactose, fructose, sucrose or esters thereof, trehalose or derivatives thereof , Dextrin, gelatin, pectin, starch, color capsule, carboxymethyl kitchen or hydroxyalkyl (C2 to C4) kitchen or chitosan, low molecular weight kitchen or chitosan with addition of alkylene (C2 to C4) oxide such as chitosan and ethylene oxide Salt, chitosan, sulfated kitchen or chitosan, phosphorylated kitchen or chitosan, alginic acid or salts thereof, hyaluronic acid or salts thereof, chondroitin sulfate or salts thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carbocycityl cellulose Rexroth, Carbocycisyl Cellulose Natrium, Hydroxyethyl Cellulose, Hydroxypropyl Cellulose, Nitrocellulose, Crystalline Cellulose, Polyvinyl Egg Polyalkylene oxides or crosslinked polymers thereof, such as polyols, polyvinyl methyl ethers, polyvinyl pyrrolidones, polyvinyl methacrylates, polyacrylates, polyethylene oxides and polypropylene oxides, carbokishibinirporima, and poriechirene It can be added by selecting more than one kind among immigration.

Addition of Organic Powder

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-paratoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) hardened fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Inorganic Pigment Addition

Anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron sulfate, red iron oxide, black iron oxide, group It may be added by selecting at least one of jaw, chromium oxide, chromium hydroxide, carbon black, caramine and the like.

Addition of free radical scavenger.

It may be added by selecting one or more of SOD, catarases, and glutathione paokisidase. '

Promotion of turnover / addition of cell vitality substances

Hydroquinone, lactobacillus extract, placental extract, ganoderma extract, vitamin A, vitamin E, aranthin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, golden, sugina, gentian, burdock, root root , Carrot, hamamarisu, hops, yokinin, audrey kosou, senbri, tuki, tukinsenka, flax tea, oat giriso, cucumber, tachija kosou, rosemary, parsley) Can be added by selection '

Add surfactant

Anionic surfactant (arkyrcarboxylic acid salt, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric surfactant: carboxylic acid type surfactant ( Amino type, betaine type), sulfuric ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant, ether ester type nonionic surfactant, ester type ratio Ionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants, other surfactants (natural surfactants, derivatives of protein hydrolysates, polymer surfactants, surfactants including titanium and silicon, hooked carbon-based Surfactant) and the like can be selected and added.

Addition of chirosinase activity inhibitor

Vitamin C or its derivatives (vitamin C phosphate ester magnesium salt, vitamin C glucoside, etc.), hydroquinone or its derivatives (hydroquinone benzyl ether, etc.), kojic acid or its derivatives, vitamin E or its derivatives, N-acecir Chirosine or its derivatives, glutathione, hydrogen peroxide, zinc peroxide, placental extract, erraic acid, arbutin, lecinonor, silk extract, plant extract (camomile, kuwa, gardenia, tuuki, waremokou, clara, mugwort, suica Zura, Kida, buckwheat, Mathode, chopsticks, Audreykosou, Hope, Sanzashi, Eucalyptus, Seiyo-no-kogirisou, Artea, Keihi, Mankeishi, Hamamarisu, Yamagwa , Bellflower, Tshishi, Inspector, Pan-Dress, Ephedra, Senkyu, Jokkat, Psycho, Bowhoo, Hamaboufu, Golden, Peony-dried Herbal Medicine, Peony, Gennoshoko, Brown root, Licorice, Mallow, Aloe, Shouma, Safflower, green tea, black tea, Sub-medicine) and the like may be selected and added.

Melanin Pigment Reduction / Decomposition

Hydrogen peroxide, zinc peroxide, hydroquinone or derivatives thereof (hydroquinone benzyl ether) and the like may be selected and added.

Addition of astringents

Select one or more of succinic acid, aranthine, zinc chloride, zinc sulfate, zinc oxide, caramine, zinc paraphenor succinate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds) Can be added.

Added Peroxide Lipid Inhibitor

β-carotene, plant extracts (sesame cultured cells, flax tea, oatgirissou, hamamarisu, chouji, merissa, enmeisou, shirakaba, salvia, rosemary, southern skyberry, aging, ginkgo, Green tea) and the like may be selected and added.

Anti-inflammatory

Ictamohr, indmethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetyrsaritilic acid, ziphhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, giaazrene, camaz It may be added by selecting one or more selected from the group consisting of ren, chloropheniramine, chloryrrhetinic acid or salts thereof, grichretinic acid or salts thereof, licorice essence, root extract, age stick kiss, propolis and the like.

Add Erasase Inhibitor

Furororinic acid isopropyl, plant extract (golden, oat girsour, clara, mulberry leaf, Keihi, Kenno Shoko, canopy, salvia, Seiyou Watko, Boiju, Botanfi), seaweed extract, etc. It may be added by selecting one or more of the.

Antiandrogen Addition

Follicle hormones (estrone, estradiol, echinir estradiol, etc.), ISO flavones, Okisendron and the like may be selected and added.

Adding blood stimulants,

Citron extract, Arnica extract, Capsicum tincture, Iris extract, ν-orizanol, Vitamin E and its derivatives, Garlic extract, Ginseng extract, Aloe extract, Gentianaekis, Tukie It may be added by selecting at least one kind from kiss, separanthin, carpronium chloride, minoxycidyl and the like.

Adding antioxidants

Vitamin E and its derivatives, Vitamin C and its derivatives, carotinoids, dibutylhydroxytoluene, butylhydroxyanisole, rutin and its derivatives, glutathione and its derivatives, superoxide dismutase (superoxide dismutase), mannitol, and an anti-inflammatory agent may be added by selecting at least one of glycyrrhizic acid and derivatives thereof, glycyrrhetinic acid and derivatives thereof, sulfur and derivatives thereof, and the like.

Add metabolic activator

At least one selected from photosensitizer No. 301, cypress thiol, pantothenic acid or a derivative thereof, aranthine, placental extract, biotin, pentadecanoic acid, and the like may be added.

Add keratin dissolving agent

Resorcin, salicylic acid, lactic acid, urea and the like can be added to select one or more.

Addition of cell activator

Examples of vitamin A and its derivatives, and anti-UV agents include paramethoxy cinnamic acid-2-ethylhexyl, 4-tert-butyl-4'-methoxydibenzoylmethane, oxybenzone and its derivatives, titanium oxide, fine titanium oxide, zinc oxide and Particulate zinc oxide and the like. Inorganic powders such as titanium oxide and zinc oxide exhibit higher effects when they are used as fine particles. Examples of the moisturizing agent include glycerin, 1,3-butylene glycol, proteins or their derivatives or hydrolysates, and salts thereof (collagen and elastin). And the like, hyaluronic acid and salts thereof, amino acids and derivatives thereof, sugars, D-panthenol and derivatives thereof, glycolipids, phospholipids, ceramides, urea, mucin, and the like.

Alcohol addition

Higher alcohols such as cetyl alcohol can be added.

Flavoring

Natural animal flavors such as Jakow, civet, castium, amber grease, anise oil, angelica oil, Iran Iran oil, Iris oil, Wikyo oil oil, orange oil oil, Kananga oil oil, Karawe oil oil, cardamom oil oil Yak Wood Essential Oil, Cumin Oil, Toothpick Oil, Cinnamon Oil, Cinnamon Oil, Geranium Oil, Copaiba Barsam Oil, Korean Del Oil Oil, Perilla Oil, Fern-Wood Oil, Citronera Oil, Jasmine Oil, Ginger Glass Oil , Samjung Oil, Spare Mint Oil, Western Peppermint Oil, Evergreen Tree Oil, Chew Billows Oil, Clove Oil, Equalization Oil, Dongrok Oil, Trvarsam Oil, Bacary Oil, Rose Oil, Parmesan Rose Oil, Cypress oil, Khiva oil, sandalwood oil, Petit Gren oil, Beige oil, Becchibar oil, Belgamode oil, Pervarsam oil, Boadrose oil, Canva oil oil, Mandarin oil, Eucalyptus oil , Lime essential oil, lavender essential oil, Linaro essential oil, lemon glass essential oil, lemon essential oil, rosemary essential oil, Hwajong peppermint essential oil and the like can be selected by one or more selected, the essential oil of the fragrance is maintained in the composition of the present invention It can also be used for inclusion.

Add fungicide

Azulene, salicylic acid, ginxylthione, benzalkonium chloride, photosensitive No. 301, sodium mononitroguicol, hinokithiol, triclosan, trichlorohydroxydiphenyl ether, resorcin, isopropylmethylphenol, chlorhexidine Gluconate, phenoxyethanol, undecylenic acid, etc. are mentioned.

Ph adjuster added

Examples of the pH regulator include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide and sodium dihydrogen phosphate. Shell calcium and the like.

Other hormones, metal ion sequestrants, chelating agents, antiseptics, antiviral agents, coolants, stabilizers, emulsifiers, animal and vegetable proteins and their seafood, animal and vegetable polysaccharides and their equivalents, animal and vegetable glycoproteins and their compounds As much as seafood, blood stimulator, anti-inflammatory, anti-inflammatory, anti-allergic agent, wound healing agent, defoamer, thickener, enzyme, purified water (electron water, small clustering), oral solvent, deodorant, deodorant, bitter flavor, seasoning By using together with these, the additive or synergistic improvement of the effect of this invention or another cosmetic effect can be anticipated.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More Preferably it is mix | blended with 0.01 to 3 weight%

The composition of the present invention, acne, eczema, psoriasis, ringworm, pruritus, allergic or atopic dermatitis, atopic acne, atopic eczema, atopic psoriasis, atopic dermatitis, atopic dermatitis, pustules, blemishes, dead skin, etc. It has the effect of treating, preventing and whitening, moisturizing and exfoliating the skin of the disease, and the external preparation of the skin can be formulated into various forms such as liquid skin, powder powder, gel, lotion, massage pack, cream, ointment. have.

Specific examples of cosmetics include face wash, face wash, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisturizing cream, milky lotion, lotion, pack, aftershave cream, sunburn prevention cream, suntan oil, soap, body shampoo , Hair shampoo, hair rinse, hair treatment, wool, hair care, hair cream, hair liquid, set lotion, hair spray, hair bridge, coloring, color spray, permanent wave liquid, press powder, loose powder, eye shadow , Hand cream, lipstick and the like.

According to the present invention, a cell product composition comprising a protein which is a constituent of an extracellular matrix (ECM) containing an intercellular signal transduction promoting substance (STPM) extracted from an animal bladder, and a method of manufacturing the same, Has an effect.

Although the following Examples, Experimental Examples, and Preparation Examples will be described in more detail, but the present invention is not limited thereto and is not limited thereto.

Example 1.

Ten pig bladders were purchased at the slaughterhouse in Eumseong-gun, South Korea.

Protein extraction

1. 3 washes-rapid freeze-crushed-lyophilized-crushed-filtered-powdered.

2. 2 washings-crushing-alcohol impregnation-stirring-filtration-lyophilization-grinding-filler-powder collection. Residual Alcohol Vaporization-Capture of STPM.

3. 3 washings-crushing-fermenter input-sap and microorganism input-fermentation-fermentation broth collection

4. 2 washes-crushing-fermenter-input-sap and microbial input-fermentation-filtering-lyophilization-powder collection.

In the above process, 106 g of STPM-containing collagen powder in step 1, 68 g of collagen in step 2, 72 g of collagen in step 3, and 0.07 g of STPM. In step 4, 59 g of collagen and 0.08 of STPM were obtained.

In the third or fourth process, after removing the impurities by primary filtering, collagen containing STPM may be used as a liquid.

Neutrality was determined by measuring pH, amino acid analysis, SAD-PAGE electrophoresis analysis, gel strength measurement, viscoelastic analysis, denaturation temperature measurement, and gel strength change with fermentation time. Measurement of strength change of gel upon freezing. Gel Formation Point and Melting Point Measurement. Viscoelastic analysis was confirmed to be collagen as an excellent protein.

Example 2.

Composition of external skin preparations and oral administration

Oral administration and composition of cell regeneration material in powder form.

The composition of Table 1 may be administered orally and may be added to the external preparation for skin.

In addition, the basic composition of the external preparation for skin in Table 1 is copper, vegetable extracts, oils, moisturizers, fatty acids, minerals, alcohols, polyhydric alcohols, emulsifier waxes, esters, vitamins, α-hydroxy acids, ultraviolet absorbers Or blocking agents, whitening agents, amino acids, gums, sugars or water-soluble high molecular compounds, organic powders, inorganic pigments, active oxygen scavengers, turnover accelerators, cell activators, surfactants, chirosin activators, Melanin reduction or degradation agents, astringents, lipid peroxidation inhibitors, anti-inflammatory agents, elastase inhibitors, anti-androgens, blood stimulators, antioxidants, metabolizing agents, keratin-soluble agents, cells The activators, alcohols, fragrances, fungicides, ph regulators may be further included.

(Table 1)

The basic composition of the cellular renewal functional skin external preparation containing STPM-containing collagen as a medicament is prepared in the form of an ointment, cream, liquid form, the composition and the amount of the composition are shown in Table 2.

(Table 2)

Composition of ointment or cream cell regeneration agent for skin external use.

(Table 2)

Composition of the cell regeneration agent composition in liquid form.

(Table 3)

Composition of wrinkle improvement functional cosmetic composition.

(Table 4)

[Experimental Example]

After purchasing molemot, after one week's normal feeding,

After performing general anesthesia using ether, hemostasis was made by cutting both hips with a mass of 2 cm, and then spraying the powder prepared in Example 1 above, and then treating the wound with petrolatum and bandages for 20 days. Was observed.

RESULTS: After 3 days, the cell texture was 20% regenerated, after 10 days, the tissue regeneration was 90%, and after 20 days, the tissue was restored to normal.

Claims (12)

ECM (<extracellular matrix>) which acts as a cushion of E cells in the tissues containing the intercellular signal transduction promoter (STPM) extracted from the animal bladder Cellular regeneration functional composition comprising a protein, which is a component of an extracellular matrix containing an intercellular signal transduction facilitator, separated from the bladder of an animal having a characteristic of 0.1-99% by weight of the protein as a drug And its manufacturing method The bladder of claim 1, wherein the bladder of the animal is at least one selected from among pigs, cows, sheep, deer, and goats. The protein of claim 1, wherein the bladder of the animal is washed with a surfactant and water, rapidly frozen and pulverized first to 100 mash-1000 mash, and then the pulverized product is freeze-vacuum dried and secondly crushed to 1,000 mash-nano size. And a method for producing a cell regeneration functional composition comprising a protein, which is a constituent of an extracellular matrix containing an intercellular signal transduction facilitator, isolated from the bladder of an animal prepared by a method of extracting by hilting. The method of claim 1, the protein is a liquid animal prepared by the method of washing the bladder of the animal with a surfactant and water, rapid freezing and pulverization, fermenting the pulverized bladder powder with wood sap and microorganisms, then filtering Cell regeneration functional composition comprising a protein isolated from the bladder as a drug. The protein of claim 1, wherein the bladder of the animal is washed, quenched and pulverized, and the pulverized bladder powder is placed in a fermentation vessel, wood sap and microorganisms are added to liquid fermentation, and the liquid fermentation product of claim 4 is lyophilized. Cell regeneration functional composition comprising a protein isolated from the bladder of the animal produced by the method as a drug. 1. The cell regenerative agent according to claim 3-5, which is an injection, oral administration, or external skin preparation. 1. The cytogenetic agent according to claim 3-5, wherein the protein isolated from the bladder of the animal contains 0.1% -99%. 1. The protein according to claim 3-5, wherein the protein is collagen which is a construct of ECM (extracellular matrix) containing STPM as an intercellular signaling substance. The collagen according to claim 8, which is collagen containing elastin and chondroitin. 1. The protein according to claim 3-5, wherein the protein comprises a sulfur-containing amino acid. The cell regeneration agent of claim 1, wherein the cellular regenerating agent, which is separated and extracted from the animal's bladder as an medicament, is absorbed from the sap of the tree or the sap fermentation solution of the tree as a protein absorber in order to absorb the animal bladder extract protein into the cells. % Contained. Infiltration of active substances into skin tissue through skin membrane ion channels by incorporating active substances into the sap of trees.