JP6000022B2 - Acne preventive / ameliorating agent and skin external preparation composition - Google Patents

Description

  TECHNICAL FIELD The present invention relates to an acne prevention / amelioration agent effective for acne prevention and its improvement or treatment, and a skin external preparation composition used for the prevention / amelioration of acne.

  Acne is a medical condition called acne vulgaris, a skin disease of pores commonly found on the face, chest, and back, mainly in puberty.

  The initial stage of acne is a condition in which the pores swell due to secretion of sebaceous glands accompanying the rise in male hormones, and the pores are closed due to detachment of the stratum corneum. The clogging of the pores is repeatedly opened and closed due to the pressure of sebum ejection, and rises from the skin surface. Acne bacteria overgrow inside the pores, inflammation occurs due to accumulation of the inflammatory substances, and redness increases. As this inflammation progresses, pus accumulates in the pores and the structure of the pores is destroyed by infiltration of immune cells. In addition, when this inflammation and destruction of the structure reaches deep pores, scars that do not disappear permanently may remain.

  When the acne generation process is considered, there are four combined aspects of sebum secretion by male hormones, pore clogging due to keratinization failure, hyperproliferation of acne bacteria, and inflammatory response.

  Acne occurs mainly in the face, for example, because it is a conspicuous skin disease that is difficult to conceal with makeup cosmetics etc., patients' desire to keep the skin clean and prevent by washing and etc. is as much as possible It appears strongly. Conventionally, as a method for preventing and improving such acne, an internal medicine, an external medicine and the like under the guidance of a dermatologist have been used.

  In addition, as a proposal of cosmetics, the use of bactericides such as salicylic acid (Patent Documents 1, 2, 3, 4, 5) and benzoyl peroxide (Patent Documents 4 and 6) that are restricted in Japan is used. Although these drugs have been proposed, they are drugs that suppress some of the causes of acne, such as sterilization.Although salicylic acid and benzoyl peroxide are effective against acne, they are highly irritating to people with sensitive skin. Has been considered unsuitable.

JP 61-72707 Japanese Patent Laid-Open No. 03-157311 JP-A-10-87496 JP 2001-206820 JP 2002-37736 A JP 61-7252

  On the other hand, from the viewpoint of low skin irritation, a relatively mild plant extract has attracted attention compared to the above-mentioned fungicides, and many plant extracts have been effective for the prevention and improvement of acne so far. Has been advocated. However, none of the effects required from the patient's standpoint has been satisfied, and it has been used as a supplement for conventionally used antibacterial agents such as acne and anti-inflammatory agents, or They were only used in combination to alleviate the stimuli they exert.

  So far, plant extracts that have been effective against acne have not been effective in terms of improvement / treatment even in a single or a mixture of several plant extracts.

  The problem of the present invention is that there is almost no skin irritation, and it can be safely used for adolescents, young people, weak skin quality, sensitive skin such as allergies, etc., and prevention, improvement or treatment of acne The purpose of the present invention is to provide an acne prevention / ameliorating agent that exhibits a sufficient effect on the skin and a skin external preparation composition (hereinafter simply referred to as a skin external preparation composition) that is used to prevent or improve acne. It is.

  In the present invention, a specific plurality of plant extracts and a specific fruit juice lactic acid bacteria fermentation solution are used from the viewpoint of low irritation to the skin. That is, the present inventor compared various plant extracts and lactic acid bacteria fermentation liquids of plant components with various observations seen in the process of acne generation, and as a result of intensive studies from each aspect, a specific plant extract And a specific fruit juice lactic acid bacteria fermented liquid, it is difficult to achieve with a conventionally known acne preventive / ameliorating agent using a plant extract, and it becomes a composition that is extremely effective for prevention, improvement or treatment. As a result, the present invention was completed.

  The present invention is a acne preventive / improving agent characterized by containing soybean extract, Giant extract, Western white willow bark extract, Oat extract, Neem extract, Dokudami extract, Ogon extract, and Western pear juice lactic acid bacteria fermentation liquid as constituents The problem is solved by an external preparation composition for skin.

  The acne preventive / ameliorating agent according to the present invention can be safely used for sensitive skin such as weak skin quality and allergies, and the effect is not only antibacterial and inflammation, but also the sebum accompanying acne occurrence. It acts markedly on all symptoms caused by secretion, clogging of pores due to insufficiency of keratinization, hyperproliferation of acne bacteria, inflammatory reaction, etc., and can be improved and treated as well as prevention of acne. Therefore, the same effect can be obtained by using the acne prevention / ameliorating agent in a skin external preparation composition.

It supplements about the plant used as an acne prevention and improvement agent by this invention.
(1) Soybean is a seed of the leguminous (Glycine) soybean “Glycine max (L.) Merrill (= G.hirsta Maxim.)”.

(2) Diou is the root of the genus Scrophulariaceae, Rehmannia, Rehmannia chinensis, or other related genera.

(3) Western white willow is Salix alba L. (= S. aurea Salisb.), Also known as white willow. Leaves, bark, branches, roots, root barks, seeds and the like can be used, but bark or leaves are particularly preferable. In addition, white willow S. jessoensis Seemen (= S. hondoensis Koidz.), Weeping willow S. babylonica L., koganesudare S. alba L. subsp. Vitellina (L.) Arcang. X S. babylonica L, kogome willow S .serissaefolia Kimura, S.yoshinoi Koidz. etc. can also be used.

(4) “Owaku” refers to bark excluding the pericarp of Rutaceae, “Phellodendron amurense Ruprecht” or other related plants. Variants P. amurense Rupr.var.japonicum (Maxim.) Ohwi; P. amurense Rupr.var.sachalinense Fr.Schm .; P. amurense Rupr.var.lavallei (Dode) Sprague ( Miyaki Hada), P. chinense Schireid. And its variants can be used as well.

(5) Neem refers to the flowers of the Melia azadirachta L. (aka Azadirachta indica Juss.) (Also known as neem, Margosa) flowers, ears, pericarp, fruit, leaves, branches and leaves. Bark, rhizome, root bark, root, seed, aerial part or whole grass are used, preferably leaves are used. In addition, Azadirachta excelsa of the same genus can be used.

(6) Dokudami is the above-ground part or whole plant of the Saururaceae genus Houttuynia or Houttuynia cordata Thunberg.

(7) Ogon refers to the roots excluding the pericarp of Labiatae, Scutellaria, and Scutellaria baicalensis Geoegi.

  These plants (1) to (7) are preferably used as they are or after being processed into a chopped or crushed dry product. Extraction from plants is performed with a solvent at room temperature to the boiling point of the solvent. Usable solvents are water, alcohols (for example, lower alcohols such as methanol, ethanol and butanol, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin), ketones such as acetone, ethyl ether, Esters such as dioxane, acetonitrile, and ethyl acetate, and organic solvents such as xylene, benzene, and chloroform can be used alone or in any combination of two or more.

  In the extraction method, the solvent 1 to 20 is usually used for the plant 1, and the immersion extraction is performed for 1 hour to 1 week at room temperature to the boiling point of the solvent under normal pressure. Further, not only normal pressure but also high pressure or supercritical fluid can be used. After extraction, the solution is recovered by filtration, and purification such as deodorization and decolorization is performed as necessary. The extract obtained in this manner may be processed as it is or as needed, such as dilution and concentration.

(8) Pear (also known as pear) is the fruit juice obtained from the peel, fruit and seeds of the Rosaceae (Pyrus) pear (Pyrus communis L.) for fermenting the juice The `` lactic acid bacterium '' to be applied is applicable to the general classification of lactic acid bacteria, `` Lactobacillus genus '', `` Streptococcus genus '', `` Lactococcus genus '', `` Leuconostoc genus '', `` Pediococcus genus '', `` Sporolactobacillus genus '', `` Bifidobacterium genus "and so on. The fungi of the genus `` Lactobacillus '' include Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, Lactobacillus delbruckii subsp.bulgaricus, Lactobacillus delbrueckii subsp.delbrueckii, Lactobacillus delbruckii subsp.lactis, Lactobacillus , Lactobacillus sanfrancisco, etc. are used, but the same genus species can also be used. Any type and any of these hybrids can be used. Streptococcus thermophilus or the like is used as a fungus of the “genus Streptococcus”, but other species of the same genus can also be used. Any type and any of these hybrids can be used. As the fungi of the “genus Lactococcus”, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. Lactis and the like are used, but the same species can also be used. Any type and any of these hybrids can be used. As the fungi of the “Leuconostoc genus”, Leuconostoc citreum, Leuconostoc lactis, Leuconostoc mesenteroides subsp. Cremoris, Leuconostoc mesenteroides subsp. Dextranicum, Leuconostoc mesenteroides subsp. Any type and any of these hybrids can be used. As fungi of the “genus Pediococcus”, Pediococcus acidilactici, Pediococcus halophilus, Pediococcus pentosaceus, and the like are used, but the same species can also be used. Any type and any of these hybrids can be used. As fungi of the “genus Sporolactobacillus”, Sporolactobacillus inulinus and the like are used, but other species of the same genus can also be used. Any type and any of these hybrids can be used. As the fungi of `` genus Bifidobacterium '', Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactentis, Bifidobacterium liberrorum, Bifidobacterium longum, Bifidobacterium parvulorum, Bifidobacterium pseudolongum, Bifidobacterium thermophilum, etc. You can also. Any type and any of these hybrids can be used.

  Fermentation conditions after inoculation with lactic acid bacteria are not particularly limited and can be arbitrarily selected depending on the type of bacteria used, but as a guide, fermentation temperature is 15 to 45 ° C, preferably 25 to 40 ° C, initial pH is 3.0 to 7.0, preferably Is performed for 4.0 to 6.5 and the culture time is 6 to 120 hours, preferably 18 to 64 hours. At this time, various nutrients including sugar, acid for adjusting pH, alkali and the like may be appropriately added. The fermentation may be mixed fermentation or continuous fermentation, and microorganisms other than lactic acid bacteria, such as yeast, may be used for mixed fermentation with lactic acid bacteria or secondary fermentation after lactic acid bacteria fermentation. After fermentation, it is filtered, and if necessary, purification treatment such as deodorization and decolorization is performed using sterilization treatment, pH adjustment, ion exchange resin, activated carbon column, dialysis membrane and the like. In addition, an organic solvent such as ethanol, propylene glycol, 1,3-butylene glycol, or any mixed solution thereof can be added. The extract thus obtained can be used as it is, but may be used after being concentrated and dried to form a paste or powder.

  In addition, the fermentation medium of the fungi used in the present invention can use nutrient components such as sugar, yeast extract, peptone, plant components, L-glutamic acid, sodium L-glutamate in fruit juice or fruit juice. It can be used alone or in combination of two or more. In addition, acid, alkali, etc. for pH adjustment may be added as appropriate, and the juice concentration is not particularly limited, but it is 1 to 30%, preferably 5 to 20% as the fruit juice-derived solid content. It is good.

  Fermentation conditions such as pH and temperature, time, and the like can be arbitrarily set depending on each bacterial species, and the bacterial species used may be a single species or a combination of two or more species. Although the culture medium after fermentation can be used as it is as a fruit juice fermentation metabolite, it is preferable to filter the culture medium after fermentation in terms of the function and appearance of the lactic acid bacteria fermentation product of the fruit juice. Moreover, it is also possible to add arbitrary operation suitably in the range which does not impair the essence of this invention, such as filtration, centrifugation, extraction, refinement | purification, and sterilization, as needed for the culture medium after fermentation. It can also be diluted, concentrated and dried.

  Solvents used for extracting the culture medium after fermentation include water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), acetone, and the like. Ketones, esters such as diethyl ether, dioxane, acetonitrile, and ethyl acetate, and organic solvents such as xylene, benzene, and chloroform can be used alone or in any combination of two or more.

  Acne preventive / ameliorating agents according to the present invention are: 1) topical or systemic external pharmaceutical preparations, skin cleansing agents, skin cosmetics, 2) medicinal or cosmetic preparations applied to the scalp / hair, 3) bath water 4) Prevent or improve acne in the form of impregnating liquids, etc. for medicinal or cosmetic bathing agents, 4) skin adhesive sheets, cosmetic sheets, cosmetic cotton, hygiene products, sanitary cotton, wet tissue, etc. Therefore, it can be used by appropriately blending in the external preparation composition for skin.

  Specifically, basic cosmetics such as creams, gels, ointments, liquid medicinal skin external preparation compositions, lotions, emulsions, creams, ointments, gels, lotions, oils, packs, mists, facial cosmetic sheets, etc. , Shaving agents, facial cleansers, skin cleansers (including soaps), shampoos, rinses, hair treatments, hair styling, hair styling, hair growth / hair restorations, foundations, lipsticks, blushers Makeup cosmetics such as eye shadow, eyeliner, mascara, and bath additives.

The following agents may be used in combination with the acne preventing / ameliorating agent and skin preparation for external use according to the present invention, if necessary.
(A) finasteroids known as male hormone activity inhibitors, cyprosterone, azelaic acid or their derivatives and salts thereof (b) glycolic acid, lactic acid, tartaric acid, malic acid, known as a stratum corneum peeling normalizing agent, Ascorbic acid, salicylic acid or their derivatives and their salts, resorcin, retinoid, whey (c) known as antibacterial agents, acrinol, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, Sulfamine, Mercurochrome, Alkyldiaminoethylglycine chloride solution, Isopropylmethylphenol, Triclosan, Kudin, Salicylic acid or their derivatives and their salts, pionine, quaternary ammonium salts, sulfur preparations, isoforms Lopylmethylphenol, lactoferrin or its hydrolyzate (d) known as anti-inflammatory agents, ictamol, indomethacin, kaolin, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone, guaiazulene, camazulene, maleic acid Chlorpheniramine, glycyrrhizic acid or a derivative thereof or a salt thereof, glycyrrhetinic acid or a derivative thereof or a salt thereof

  When producing a skin external preparation composition using the acne prevention / ameliorating agent according to the present invention, in addition to the above components, the following are generally used for external preparations such as cosmetics, quasi-drugs, and pharmaceuticals. Components can be appropriately blended.

(Various oils and fats)
Avocado oil, almond oil, fennel oil, egoma oil, olive oil, orange oil, orange rafa oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, cucumber nut oil, safflower oil, shea fat, large Bean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, owl, palm oil, beef tallow Or hydrogenated products of these oils and fats (hardened oil, etc.).

(Wax)
Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax.

(mineral oil)
Liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, micro-cristan wax, squalene, squalane, pristane, etc.

(Fatty acids)
Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid , Synthetic fatty acids such as 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(Alcohol)
Natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol and phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol and 2-octyldodecanol.

(Polyhydric alcohols)
Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol , Glycerin, pentaerythritol, sorbitol, mannitol, etc.

(Esters)
Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, phthalate Dibutyl acid, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

(Metal soap)
Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

(Gum, sugars and water-soluble polymer compounds)
Gum arabic, benzoin gum, danmar gum, guaiac gum, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, Carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2 to C4) chitin or chitosan to which alkylene (C2 to C4) oxide such as ethylene oxide is added, low molecular chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorus Oxidized chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxy Polyalkylene oxide such as chill cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide and polypropylene oxide or Its cross-linked polymer, carboxyvinyl polymer, polyethyleneimine, etc.

(Surfactant)
Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkylamine salt, alkyl quaternary ammonium salt), amphoteric surfactant: carboxylic acid Type amphoteric surfactant (amino type, betaine type), sulfate type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic surfactant) Agents, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing type nonionic surfactants), other surfactants (natural surfactants, proteins) Hydrolyzed derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbon surfactants Such as.

(Various vitamins)
Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), Pyridoxine (vitamin B
6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotins, choline, inositol, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D2), cholecalci Ferrol (vitamin D3), dihydrotaxosterol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), In addition, essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

(Various amino acids)
Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine And amino acid derivatives such as sulfates, phosphates, nitrates, citrates, or pyrrolidone carboxylic acids.

(Various additives derived from plant or animal materials)
These are processed in a conventional manner according to the type and form of the product to be added (eg grinding, milling, washing, hydrolysis, fermentation, purification, pressing, extraction, fractionation, filtration, drying, pulverization , Granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., may be arbitrarily selected and combined), and may be arbitrarily selected from various materials.

  The solvent used for the extraction may be selected in consideration of the intended purpose and type of the product to be provided, or the processing performed later. Usually, water, a water-soluble organic solvent (for example, ethanol, propylene glycol) , 1,3-butylene glycol, etc.) is preferably used. However, when it is not preferable to contain an organic solvent depending on the use, it is sufficient to use only water or ethanol that is easy to remove after extraction, and it may be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.

  In addition, when various additives derived from plant or animal materials are applied to systemic or topical skin external preparations, it not only protects skin and hair, but also moisturizes, improves touch and texture, and imparts flexibility. , Relaxation of stimulation, relaxation of stress due to fragrance, cell activation (prevention of cell aging), suppression of inflammation, improvement of skin quality / hair quality, prevention of rough skin and its improvement, hair growth, hair growth, prevention of hair loss, imparting luster, cleanliness In addition to cosmetic effects such as effects, fatigue relief, blood flow promotion, and warm bath effects, effects such as flavoring, deodorizing, thickening, preserving, and buffering can also be expected.

Specific plants (herbal medicines) used as raw materials include, for example, almond (amynna), ai (indigo leaves), aokazura (Keifu Fuji), aotsuki-rafuji (wood moth), aonorus agave, fuchlin agave, acacia, acassuri fruit, Red grapes, redwood (logwood), red-crowned wrinkles, red radish (radish grass roots), red radish (dianthus), agi (Aki), echini (crustacean), akebi (kitsu), asa (asako) , Maruba morning glory or morning glory (seasoned cow), Hydrangea (hydrangea), Achacha (Amacha), Ashitaba (Asuka leaves), Amana (Ako), Acerola, Anise, Abemaki fruit, Avocado, Ama, Achacharu, Amadokoro (Tamatake), Amigasayuri (shellfish mother), Aloe Vera, Angelica, Anzu Honganzu (Anjin), Ansokkou (benzoic incense), Igakouzorina (Gallhead) Rush (shinshin grass), Japanese knotweed (tiger cane), strawberry, fig (no flower fruit) or its leaf, Ichihatsu (first), Ichibibi (winter lion), Ichiyakusou (one medicinal herb), Ichihahagi (distant), locust bean, rice seeds or Seed coat, Inondo seed, Ikisakuso, Nettle, Ylang-Ylang, Fennel (Yellow perfume), Spiraea (Sanryo), Astragalus fruit, Amaranthus japonica, Turmeric (Urbanus), Usubasaicin, Keirinsaicin (Spicy), Usbeniah, Usbeni Hollyhock, deer (summer hay), Japanese apricot (plum) or its flesh, radish, oolong tea, walrus (redberry), Eunsumikan (skin peel), estragon, Ekokogi (蝦 夷 goka), echinashi (Hosobamurakibarengikue), Echinacea榎 茸), Elderberry fruit, Elemi, Elingy (Prerotor) Erinji), Enju (槐花, 槐花 米), Ogi, Kibana-ogi (Yellow), Oulen (Yelen), Pteropus lily (Karonin), Otsu Rafuji (prevention), Psyllium (pre-car, pre-cursor), Toucan ( Eggplant), Ovanaokera, Okera (white birch), Obayayashabushi fruit, Sea hawthorn hawthorn (Yamakoko), Ousei (Naruyori, Kagura-mabanaruyori), Sasayukiyuki, Sasanoyuki, Barley, Grained Grass (Turnover), Okra fruit, Panax ginseng, Tochibaninjin (carrot), Onamomi (Cotton ear), Onigurumi, Onino Yagara (Tempa), Onyuri Sasayuri Hakatayuri (Yuri), Onionis, Halibut (Flyer), Ominaeshi (defeat) Soy), Dutch mustard (watercress), Dutch jellies, Dutch honey bees, olives (fruit, seeds, leaves), oregano, me Di-fruit or pericarp, Silkworm (mature ground yellow), cacao seeds, oysters or their leaves (bamboo shoots), scallops (fish potatoes), cassia, casino berries (fruits), gadgets (bamboo shoots), oak (Leaf), cascarilla, cynomolgus (gold sato), valerian (valerian), birch or fern birch (white birch), pumpkin, kapok seed, kahoku salamander (sweet potato), gama (claw yellow), chamomile, roman chamomile, chamomile ( Tsutsumo), Kamukamu / Duck, Duck (Camellia), Crowd Bisaku (Semi-Summer), Oats, Garana Seeds, Calahoo (Park), Karaya, Karin (Kiso), Garcinia, Kawaratake, Ishikawa Rana (Isotake) , Buckwheat), Chinese mugwort (boiled chin), kansui (kansai), cantalara, candelilla, kanto, kanna, raspberry (Ezo strawberry, Dutch strawberry) Shrimp Strawberry, Narrow White Strawberry, Momiji Strawberry, European Red Strawberry), Kiwi Fruit, Kikarasuuri (Gurone), Chrysanthemum (Chrysanthemum Flower, Striped Ginkgo, Chrysanthemum), Kisage (Kashimi), Shigegishi (Yotei), Kizuta, Kizuta, Kidachi aloe, Kidachi pepper, Kinanoki (Cinchona, Red linden), Gymnema sylvesta, cabbage, cabbage immature fruit, cucumber, Kiraja saponaria, Kiraya, Kiranso (Kinza licorice), kumquat fruit, betel, Kimizuki (Sentsukusa), guava fruit, Guayule, cucumber nuts, wolfberry (bamboo shoots, coconut palm, cocoon leaves, crustal skin), cedar casserole (Tenmon winter), camphor tree, gooseberry fruit, gardenia (mountain lion), kunugi (bamboo grass), kumazasa, pine moth (hortiform grass), Clara (bitter), cranberry fruit, chestnut or its astringent skin, kurukuri・ Ratifolia fruit, grapefruit, buckthorn, black beetle (necessary), clove (clove, clove), kei-gai (cage, cocoon), celosia (chicken flower, chicken clover), bay cabbage (laurel tree), Kenashi Sartorii rose (crust, Mountain return), Gentiana, Kouji / Tachibana / Obenimikan / Ponkan / Santara (Tachibana skin), Koshinbara (moon flower), Kousei mint, Kozo fruit, Kocha (tea), Kohone (river bone), Kohon (Kanhon, Karamoto) , Kouliang, Cilantro fruit, Cowberry (Etsutachibana), Coconut fruit, Goshuyu (Gyuyu), Goshostrawberry (Pepper), Pepper (Pepper), Copaiba balsam, Coffee beans, Kobushi magnolia (Cotton), Burdock (Beef bowl, Gyudon) Child), kobane eel, sesame (sesame), sesame seedling (genus ginseng), trash (gomiko), sanekazura, binankazura, matsubusa, Wheat (wheat), rice, rice bran (red cocoon, white birch), rice oil, cola acminata seeds, cola vera seeds, fenugreek fruit, colombo, condurango, konjac, sisal, sisal asa, saxima button leopard (wei Sensen), cherry ( Leaf, flower, fruit, bark (cherry bark), cherry, pomegranate, sasa, sasanka, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree, cherry tree (Bamboo shoots), sweet potatoes, sugar cane, sugar beet, sunflower jujube (acid vine), saffron (banju blossom, west red blossom), pomelo fruit, savonso, salmon berry, salamander, salvia (sage), ragweed (mountain) Chinese cabbage), Sawagurumi (wild walnuts), Carrot (Ginseng), Sanshuyu (Yamamuro), Sansho (Yamamuro), Sandskon (Yamazune), Shia (Kalite), Cyano fruit, Shiitake mushroom (Shitake), Zion (purple lees), Jikitaris, Sikunshi (Amiko) , Shiso Aojiso Chirimenji Katamenjiso (Shiyo leaf, Shiso child), Rosewood, Shinanoki, Sinahoonoki, Shirarenyou (ream), Shimeji (Hinshimeji, Shakashimeji, Hatake-Shimeji, Oshiro-Shimeji, Buna-Shimeji, Shirotamotake-mochi) (Glaze), shajin (shasan), jade beard (barley winter), juzudama, palm fruit, ginger (ginger), ginger (salmon, root), shozuk fruit, shirakashi seeds, white butterfly seeds, white squirrels , Shirobanatsuta flowers, Shirominanten (Minami Tenmami), Cinchona Sacylbra, Jincho Gue (Mizuka, Mizuka, Shencho), Cinnamon, Watermelon (Sairan), Sorrel (Acami), Swimberry fruit, Stevia, Strawberry fruit, Suzu-Psycho (Xucho), Suginah (question), Surihiyu (horse tooth tendon, Horse tooth (Kenshi), Plum fruit, Japanese red pine cone, Japanese larch, Japanese kizuta, Japanese walnut, Japanese hawthorn, Japanese dandelion, Japanese horse chestnut (Mallonier), Japanese pear fruit, Japanese yellow cherry, Japanese elderberry (Elder), Japanese juniper (Juniper)・ Etsumatsu), rose, pearl butterfly, mistletoe, yellow mint, yellow mint, horseradish, peony (Ishizone), mallow, hirosanega, senega, seri, celery, sendan, senburi (this drug), senna fruit Or leaves, sennin Cormorant (Daisatsu), Soka (grass), buckwheat seeds, Somerna potato, Daio (Dahi), Daikon radishes, Daidai (orange peel, walnuts), Takatodai (Daigai), Takakawarabi (Cirrus), dark sweet cherry fruit, hollyhock , Tachikakuso (Thyme, Perilla), Tachiyanagi, Tamarind Seed, Onion, Tamshiba (Pepper), Taranoki or its root bark, Tanjin, Dandelion (Sankou) or Shirobana Dandelion, Mokodanpopo, Danmaru, Cherry fruit, Chigaya or Its root (Sone), Chixetsu carrot (Bamboo ginseng), Chicory, Ginseng (Dahi), Korean ginger (Ginseng), Ginseng (Ginseng), Ginseng (Ginseng leaves), Choreimaitake (Tsumami) , Tsukuritake (mushroom), camellia, camellia, clover (lacquered grass), camellia (duck grass), azuki bean (red bean), tsuruna ( Apricot), Tsuru carrot (Yoba ginseng), Azabuki, Deco, Teuchigurumi, Duberry fruit, Tengusa, Tencha (boiled tea), Tendai Yaku (glaze), Toga (winter coconut), Pepper (banyu), Tokinsenka (marigold), Maize or corn hair (Southern cocoon hair), Japanese horned potato (Sadako), Torindo (dragon gall), Tokusa (wood band), Tokon (gump root), Toshishi-Mamedaoshi Neashikazura, Tochu (Tochu, Tochu), Toneriko ), Tomato, tragacanth, triacanthus seeds, tormentilla, dronoki, taro aoi, nigerian berry fruit, yam yam (wild boar), nagii kada (butcher bloom), naginata koju, nazuna, rapeseed, jujube, jujube (Sakurako), Nameko, Naruko Yuri (yellow spirit), peanut (peanut), niga Fruits, flowers or stems, (garlic), garlic (large persimmon), Nurde, (bitter tree), Japanese mugwort (bitter melon), nutmeg, Kay, Nikkei (cinnamon bark), Keishi (Katsura branch), leeks (lion) (Penta), leek, white thistle (large coral), yarrow, nodake (former cucumber), wild rose, peach, palm, pineapple fruit, hibiscus (bushouge, furinbusouge, roselle), hakamabo labushi (bone crush), hakusen (white crust) ), Hakuru Berry Fruit, Jacobe (Fresh), Hazel (Chicken), Hashiridokoro (Roth Root), Basil, Lotus (Lotus, Lotus Meat, Lotus), Parsley (Netherlands Jelly), Hakamagi, Batata, Hachiku Madake (Bamboo Mushroom) , Patchouli, mint (light load, light load), flower moth (wisdom mother), banana, hana mint, hanabashi (shitsuri, shitsutsuri), vanilla bins, papaya, scallops (hub grass), hub grass, paprika, hamago Umi Mitsuba Clam (Vulgaris), Hamasuge (Katsukiko), Habibishi (Shitsuko), Hermanus (Maika Flower), Rose (Rose), Agaric (Agaric, White-faced Agaric, Uskimorinokasa), Parietalia, Harunile (Crustacea) , White skin, green leaves), breadfruit, lizard carrot (participant), pecan nuts, longan beetle (stone wall, vine pearl flower), hishi (rhizomi), pistachio, beet, honey tree, stone wall fern, Hinata Kozuchi (cow knee), cypress, hiba, castor, sunflower, bell pepper, himegama (incense bud), himematsutake (prickly agaric mushroom, hiromatsutake), pimenta fruit, juniper, sandalwood, yellow-bellied fruit, broad-tailed oyster, white-headed cypress (枇杷, 枇杷葉), betel wax (large clothes, coconut), Japanese quail (southern rattan), Japanese pheasant, Japanese dandelion (Japanese winter flowers, Japanese winter leaf), Fujibaka Maize (Orchid), wisteria bean (peel), grape fruit or pericarp / seed / leaves, beech, Fuyumushinatsutake (Cycium edulis), Brazilian licorice, Brazilian carrot, black currant fruit, blackberry, plum fruit, fluceraria, blueberry ( (Prunus), prune, blond psyllium, bundo (mung bean), loofah, henken, belladonna, berry fruit, pervian burger, boysenberry fruit, spinach, physalis (Torone), honoki (Japanese rustic, pak), bokeh ( Kiso), Hosobanaokera (蒼朮), Hosobabarengiku, Hops, Jojoba, White berry fruit, Honshimeji, Mizurutennansho (Tennansei), Maitake (Maiko), Macadamia nuts, Macri (Seaman grass), Magnolia sprengeri, Matabi ( Wood tengu), Matsukasa, Matsuba, Mayoram Malvanojadine (bitter ginseng), mulberry fruit, quince, mango, mangosteen, mango gurumi, mandarin fruit, mandarin fruit (ganoderma), citrus fruit (fruit), mandarin orange (shiba), mizokakushi (half-sided lotus), misohagi Chikuma), Mitsuwashi, Mitsuba, Green mint, Mimosa, Mioga, Miracle fruit fruit, Myrrh, Milobaran, Mugge (Mushroom), Mukunoki, Mukuroji, Murasakikikibu, Omurasakikib (purple pearl), (Murasaki maize, Mehajiki (benuse) Meowugi, Melaleuca, Merirot, Melon fruit, Moukomomogi, Mosouchiku, Mokko (wooden incense), Japanese maple peach, Peach leaf (peach leaf) / fruit / seed (peach seed), Moyashi, Morello cherry fruit, Morohaya (burlap), Yakawa Purple potato, yakchi (masutachi), cornflower (cornflower), ya Rumahakka, Yachiyanagi, Millipede (octagonal gold plate), Mistletoe (willow parasitism), Willow tree leaf, Yabgarashi, Yabukouji (purple gold beef), Japanese burdock (shoku land), Yamahanoki (yamabe), Japanese yam (Japanese apricot), Japanese mugwort, Yucca・ Flevifolia, yuzu fruit, lily, yorogusa, lime fruit, rye, rakanka fruit, raspberry leaf / fruit, lavender, longan (longan), ryokcha (green tea), apple fruit, gentian, lubus suabisimus (cool), lettuce, Red currant fruits, lemon fruits, lemongrass, forsythia, forsythia, wax palm, locon (reeds: roots), loganberry fruits, rose hips (no roses), wasabi, and warm mocows (ground).

  In addition, the raw materials derived from animal materials include chicken crown extract, pig / cow stomach, duodenum or intestinal extract or degradation product thereof, pig / cow spleen extract or degradation product, pig / cow brain Extracts of tissues, collagen derivatives such as water-soluble collagen and acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its degradation products or their derivatives, silk proteins and their degradation products Or their derivatives, porcine / bovine blood cell protein degradation products (globin peptides), porcine / cow hemoglobin degradation products (hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and its degradation products or derivatives thereof, Non-fat dry milk and its decomposition products or derivatives thereof, lactoferrin or its decomposition products, chicken egg components, fish decomposition products, etc.

(Microbial culture metabolites)
Yeast metabolites, yeast extract, rice fermented extract, rice bran fermented extract, Euglena extract, trehalose or its derivatives.

(Inorganic pigment)
Silica, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide , Gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(UV absorption / screening agent)
p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, Allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin and the like.

(Astringent)
Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds).

(Active oxygen scavenger)
SOD, catalase, glutathione peroxidase, etc.

(Antioxidant)
Ascorbic acid and salts thereof, stearic acid ester, tocopherol and ester derivatives thereof, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol , Sesamorin, gossypol, etc.

(Humectant)
Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salt, polyethylene glycol, chondroitin sulfate and its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid and its salt, sodium lactate, mini sasanishiki extract, etc.

(Peripheral blood flow promoter)
Vitamin E and its derivatives, assembly extract, garlic extract, carrot extract, gentian extract, toki extract, cephalanthin, carpronium chloride, minoxidil and the like.

(Local stimulant)
Pepper tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(Metabolic active agent)
Photosensitive element 301, hinokitiol, pantothenic acid and its derivatives, allantoin, biotin, pentadecanoic acid glyceride and the like.

(Anti-seborrheic agent)
Pyridoxine and its derivatives, sulfur, vitamin B6 and the like.

(Oxidant)
Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, sodium orthophosphate Sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(Fragrance)
Natural animal flavors such as musk, civet, castrium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, cumin essential oil, black letter Essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriander essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, dairy perfume essential oil, Tuberose Essential Oil, Clove Essential Oil, Orange Flower Essential Oil, Winter Green Essential Oil, Truval Sam Essential Oil, Batule Essential Oil, Rose Essential Oil, Palmarosa Essential Oil, Agate Essential Oil, Hiba Essential Oil, Sandalwood Essential Oil, Petit Glen Essential Oil, Bay Essential Oil, Vetiver Essential Oil, Bergamot Essential Oil, Pe -Plant flavors such as balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil, and other synthetic flavors .

(Dye and colorant)
Red cabbage dye, red rice dye, red dye, anato dye, squid dye, turmeric dye, enju dye, krill dye, amber dye, caramel, gold, silver, gardenia dye, corn dye, onion dye, tamarind dye, spirulina dye, Whole buckwheat pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(Other)
Moisturizers, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their degradation products, animal and vegetable polysaccharides and Its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, anti-allergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, deodorizers A deodorizing agent, an enzyme, etc. are raised, By using together with these, various additive and synergistic effects can be expected.

Production of plant extract and lactic acid bacteria fermentation broth (soybean extract)
For soybean extract, add 100 g of purified water to 10 g of dried soybean seeds, extract by heating, add 1,3-butylene glycol to the extract obtained by filtration to a final concentration of 30%, and filter again. Was obtained. The solid content was about 0.5%.

(Geo extract)
The Giant extract was obtained by adding 100 g of 50% 1.3-butylene glycol aqueous solution to 10 g of dried red gallows root, immersing for 1 week, and filtering. The solid content was about 2%.

(Western Willow Bark Extract)
Western white willow bark extract was obtained by adding 100 g of 30% 1.3-butylene glycol aqueous solution to 10 g of dried western white willow bark, immersing for 1 week, and filtering. The solid content was about 2.5%.

(Owaku extract)
The buckwheat extract was obtained by adding 100 g of 50% 1.3-butylene glycol aqueous solution to 10 g of dried yellowfin bark, immersing for 1 week, and filtering. The solid content was about 1.0%.

(Neem extract)
The neem extract was obtained by adding 100 g of 50% 1.3-butylene glycol aqueous solution to 10 g of dried Melia azadiracta leaves, immersing for 1 week, and filtering. The solid content was about 1.0%.

(Dokudami extract)
The dokudami extract was obtained by adding 100 g of 50% 1.3-butylene glycol aqueous solution to 10 g of the above-ground portion of the dried flowering dokudami, soaking for 1 week, and filtering. The solid content was about 1.0%.

(Ougon extract)
Ougon extract was obtained by adding 100 g of 50% 1.3-butylene glycol aqueous solution to 10 g of dried root of Scutellaria, soaking for 1 week, and filtering. The solid content was about 1.0%.

(Western pear juice lactic acid bacteria fermentation liquid)
Western pear juice lactic acid bacteria fermentation broth was obtained by ingesting Lactobacillus plantarum into 100 g of fruit juice obtained by squeezing fresh pear fruit, and then cultivating 1,3- Butylene glycol was added to a final concentration of 30% and obtained by filtration. The solid content was about 8.0%.

Manufacture of acne prevention / amelioration agent As a result of comparing various plant extracts and plant fermentation broth with various observations seen in the process of acne development, there is almost no skin irritation and comprehensive prevention and improvement of acne Best results were obtained. The case was as follows. In addition, among the constituents that are essential in the present invention, regarding the oak extract, it was found that concentration dependency strongly appears in the acne prevention / improvement effect when the ratio is changed. As a preferred embodiment, acne is prevented or improved by being contained 3 to 8 times with respect to each plant extract as another constituent and about 8 to 12 times with respect to the fermented lactic acid bacteria of pear juice. As a result, the best results were obtained.
weight%
(1) Soybean extract 2.0
(2) Giou extract 2.0
(3) Western white willow bark extract 2.0
(4) Oat extract 10.0
(5) Neem extract 2.0
(6) Dokudami Extract 2.0
(7) Ogon Extract 2.0
(8) Western pear juice lactic acid bacteria fermentation liquid 1.0
(9) Glycolic acid 5.6
(10) 1,3-butylene glycol 30.0
(11) Isopropylmethylphenol 0.25
(12) Polyoxyethylene (60E.O.) hydrogenated castor oil 4.0
(13) Glyceryl caprylate 1.0
(14) Ethylhexylglycerin 1.0
(15) The remaining amount (production method) components (10) to (14) to be purified water 100 are mixed. Add about half of component (15) to this and mix. Next, components (1) to (9) are added one by one and mixed. Finally, the remainder of component (15) was added and mixed to obtain a pimple prevention / amelioration agent.

Acne growth inhibition test The growth inhibitory effect of the acne prevention / ameliorating agent of the examples on acne bacteria (Proteonibacterium acnes) was measured. Salicylic acid and benzoyl peroxide were used as controls.
(Test method) Acne bacteria previously anaerobically cultured were uniformly applied to a GAM agar medium having a diameter of 100 mm, a paper disk having a diameter of 8 mm was placed in the center, and the paper disk was diluted to 3% with DMSO. An anaerobic culture was performed for 48 hours immediately after the acne preventive / ameliorating agent, salicylic acid, and benzoyl peroxide solution of Examples were dropped. The effect was determined by measuring the diameter of the formed growth inhibition circle to determine the acne growth inhibitory effect.

(Test results) As shown in Table 1, the acne prevention / amelioration agent of the present invention showed a stronger acne growth inhibitory effect than the antibacterial agents salicylic acid and benzoyl peroxide.

Corneal delamination test The stratum corneum exfoliation of the subject's arm, with written consent in accordance with the Declaration of Helsinki, was measured.
(Test method) A dihydroxyacetone solution was applied to the subject's arm, and the stratum corneum was stained black. Each 3% aqueous solution of the acne prevention / ameliorating agent and salicylic acid of Example was applied to the stained site at 0.1 mL once, twice a day for 7 days. Judgment of the effect was carried out by measuring the color tone of the stained region and the non-stained region with a color difference meter, and calculating the color tone of the stained region when the color tone of the non-stained region was set to 100, and evaluating the delamination .

(Test results)
As shown in Table 2, the acne prevention / amelioration agent of the present invention disappeared sooner than the antibacterial agent salicylic acid, and showed a strong stratum corneum peeling effect.

Safety test (skin irritation test)
A 24 hour closed patch test was conducted on 24 male and female monitors using samples prepared with purified water so that the acne prevention / amelioration agent produced in the examples was 5 v / v% and 10 v / v%. . Using a patch tester “Torii”, the sample was subjected to occlusion on the left and right upper arm flexors for 24 hours for determination. The sample was removed at the time when 24 hours had elapsed after the application, and after 24 hours, 48 hours, and 1 week later, the skin condition was observed and judged, and the results shown in Table 3 were obtained.

(Criteria) Judgment was performed according to the following criteria.
No reaction 0 points Minor erythema 1 point Evident erythema 2 points Erythema and swelling, erythema and papule 3 points Other above-mentioned small water bubbles 4 points Debris with marked erythema 5 points

Manufacture of skin external preparation composition Various skin external preparation compositions were manufactured using the acne preventing / ameliorating agent of the present invention. The prescription example is as follows.

1) Production of lotion A lotion was produced according to the following formulation.
weight%
1. Acne preventive / ameliorating agent 3 of the present invention
2. Glycerin 1
3. Ethanol 5
4). Perfume appropriate amount 5. Purified water 100

2) Manufacture of emulsion The emulsion was manufactured by the following prescription.
weight%
1. Acne preventive / ameliorating agent 3 of the present invention
2. Squalane 3
3. Vaseline 2
4). Stearyl alcohol 0.3
5. Sorbitan monostearate 1.5
6). Polyoxyethylene (20) sorbitan monooleate 3
7. 1,3-Butylene glycol 10
8). Preservative (methylparaben) Appropriate amount 9. Purified water 100

3) Production of cream A cream was produced according to the following formulation.
weight%
1. Acne preventive / ameliorating agent of the present invention 5
2. Vaseline 8
3. Squalene 20
4). Cetanol 5
5. Glycerol monostearate 2
6). Polyoxyethylene (20) sorbitan monolaurate 2
7). Ceramide 3 0.5
8). Sodium monohydrogen phosphate 0.85
9. Glycerin 5
10.1,3-butylene glycol 5
11 Dimethylpolysiloxane 3
12 Purified water 100

4) Production of ointment An ointment was produced according to the following formulation.
weight%
1. Acne preventive / ameliorating agent 2 of the present invention 2
2. Glycerin 10
3. Liquid paraffin 10
4). Glyceryl ether 1
5. Cholesteryl isostearate 3
6). Remaining amount of white petrolatum 100

5) Production of hair shampoo A hair shampoo was produced according to the following formulation.
weight%
1. Acne preventive / ameliorating agent 1 of the present invention
2. Lauryl sulfate triethanolamine 5
3. Polyoxyethylene lauryl ether sulfate Na 12
4. 1,3-Butylene glycol 4
5. Lauric acid diethanolamide 2
6). Arginine or its salt 0.2
7). Preservative (ethyl paraben) Appropriate amount 8. Purified water 100

6) Production of body soap A body soap was produced according to the following formulation.
weight%
1. Acne preventive / ameliorating agent of the present invention 5
2. Potassium laurate 15
3. Potassium myristate 5
4). Propylene glycol 5
5. Hydrolyzed collagen solution 3
6). Sodium lactate 0.1
7). pH adjuster appropriate amount8. Purified water 100

(Clinical study) Acne improvement test 42 men and women aged 18 to 25 years with mild to moderate acne on the face in accordance with the Declaration of Helsinki and obtained written consent A clinical trial using the lotion produced in the examples was conducted under the supervision of a dermatologist. As a comparative test, a comparative lotion was used in which the acne preventing / ameliorating agent of the present invention in the lotion was replaced with salicylic acid (3%).

  Each lotion was applied to the face 0.2 mL once, twice a day for 8 weeks, and the dermatologist diagnosed the acne condition before the start of the test and after 8 weeks to determine the effect. . Judgment of the effect is based on GAGS (Global Acne Grading System), and the dermatologist classifies the subject's severity into 4 grades (mild, moderate, severe, very severe), and 8 weeks before the start of the study. The improvement status was determined by comparing later severity.

  As shown in Table 4, the severity of the subjects before the start of the test was 16 moderate and 5 mild. As a result of using a lotion containing 3% of the acne preventive / ameliorating agent according to the present invention for 8 weeks, the moderate level decreased to 4 people, the mild level increased to 17 people, and an obvious improvement in the severity of acne was observed. . In addition, a slightly moderate decrease in the number of subjects and a slight increase in the number of subjects were observed in the lotion containing 3% of salicylic acid as a comparative test. The use of a lotion was more prominent.

  The acne preventive / ameliorating agent according to the present invention hardly exhibits skin irritation and exhibits a very remarkable effect. Therefore, it can be safely used for sensitive skin such as weak skin quality and allergies. Its effects are not only antibacterial and inflammation, but also the secretion of sebum accompanying acne, It acts markedly on all symptoms that occur due to the combination of obstruction, acne overgrowth, inflammatory reaction, etc., and it can be improved and treated as well as preventing acne. Therefore, it is extremely excellent in utility as a skin external preparation composition used for preventing and improving acne.

Claims (2)

As a component,
1 part by weight of soybean extract,
1 part by weight of ziou extract,
Western white willow bark extract 1 part by weight,
1 part by weight of neem extract,
1 part by weight of dokudami extract,
1 part by weight of ougon extract,
3-8 parts by weight of agate extract,
Western pear juice lactic acid bacteria fermented liquid is 1/12 to 1/8 parts by weight with respect to parts by weight of Oat extract
Acne preventive / ameliorating agent characterized by containing in the ratio . A skin external preparation composition for use in preventing or improving acne, which comprises the acne preventing / ameliorating agent according to claim 1 .