KR20220078838A - Antioxidant and antibacterial cosmetic composition containing an extract of a fermented natural medicinal plant extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermented medicinal plant having antioxidant and antibacterial functions, and since the fermented medicinal plant of the present invention has antioxidant and antibacterial functions, it may be usefully used as a cosmetic composition.

Description

Antioxidant and antibacterial cosmetic composition containing an extract of a fermented natural medicinal plant extract

The present invention relates to a cosmetic composition comprising a fermented medicinal plant having antioxidant and antibacterial functions.

A medicinal plant is a plant that has medicinal effects on humans and animals by using the whole plant or leaves, stems, roots, etc. Among them, those cultivated for medicinal or herbal use are called medicinal crops. Medicinal plants (crops) are representative herbs that are often talked about in oriental medicine. A wide variety of plants, including woody plants and herbaceous plants, are classified as medicinal plants, and there are cases where the chemical components contained as medicinal resource plants are used for medicinal purposes, and there are cases where secretions are used medicinally.

For the development of cosmetics using medicinal plants, various studies are being conducted from confirming traditional efficacy to methods of increasing active ingredients through identification of scientific effects and identifying new effects. In particular, based on the usefulness of various medicinal plants, Therefore, it is used as a cosmetic composition.

Medicinal plants may have useful effects and toxicity of their own. Accordingly, the present inventors have completed the present invention by establishing a manufacturing process of a cosmetic composition that enhances functionality while making fragrance soft by fermenting medicinal plants.

(001) Republic of Korea registered patent KR 10-0964428 (002) Korean Patent Registration KR 10-0803925

The present inventors have prepared a fermented medicinal plant containing licorice root, champagne root, mulberry root, eoseongcho, cheongung, chrysanthemum root, long-leaved rhubarb root, mint, cornus fruit, sauerkraut root and red ginseng, and the fermented medicinal plant has antioxidant effects and It was found that it can provide an excellent antibacterial effect, and the functionality as a cosmetic material was confirmed.

Accordingly, an object of the present invention is to provide an antioxidant and antibacterial cosmetic composition comprising a fermented medicinal plant as an active ingredient.

In order to achieve the above object, the present invention is 1) licorice root, chamomile, mulberry root, eoseongcho, cheongung, chrysanthemum root, jangyeop rhubarb root, mint, cornus fruit, sauerkraut root and red ginseng at a temperature of 85 to 95 ℃ 4 to Preparing a medicinal plant extract by hot water extraction for 6 hours; 2) concentrating the extract of step 1) to 12 to 15 brix; 3) Lactobacillus casei ( Lactobacillus casei ) or Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) Inoculated and primary fermentation for 48 to 72 hours at 32 to 38 ℃; 4) Secondary fermentation of the fermented product of step 3) at 24 to 30° C. for 5 to 10 days; 5) centrifuging the secondary fermentation product of step 4) to obtain a supernatant and filtering; 6) concentrating the filtrate of step 5) at 40 to 50° C. to 35 to 45 brix; and 7) sterilizing the concentrate of step 6) at 90 to 100° C. for 30 to 90 minutes.

The medicinal plant extract is 20 to 30 parts by weight of licorice root, 20 to 30 parts by weight of licorice root, 10 to 15 parts by weight of mulberry root, 10 to 15 parts by weight of Eoseongcho, 5 to 10 parts by weight of cheongung, 5 to 10 parts by weight of chrysanthemum based on the total weight. 8 parts by weight, 3 to 7 parts by weight of long leaf rhubarb root, 1 to 5 parts by weight of mint, 3 to 7 parts by weight of cornflower fruit, and 1 to 3 parts by weight of red ginseng.

In addition, the present invention provides a fermented medicinal plant prepared by the above method.

In addition, the present invention provides an antioxidant and antibacterial cosmetic composition comprising a fermented medicinal plant as an active ingredient.

In the cosmetic of the present invention, in addition to the above essential ingredients, other ingredients usually blended in cosmetics may be blended as needed.

Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, A blood circulation promoter, a cooling agent, a restrictive agent, purified water, etc. are mentioned.

Examples of the fats and oils include ester fats and oils, hydrocarbon oils, silicone oils, fluorine oils, animal fats, and vegetable oils.

As ester-based fats and oils, tri2-ethylhexanoate glyceryl, 2-ethylhexanoate cetyl, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(caprylic acid, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, tetra 2-ethylhexanoate pentaellithritol , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostea laurate Lil, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearic acid isopropyl, 2-ethylhexanoate cetoster Aryl, 2-ethyl stearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(caprylic acid, capric acid) propylene glycol, dicaprylic acid propylene glycol, dicapric acid Neopentyl glycol phosphate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecyl, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , isononanoate octyl, neodecanoate hexyldecyl, neodecanoate octyldodecyl, isostearate isocetyl, isostearyl isostearate, isostearic acid octyldecyl, polyglycerol oleate, polyglycerol isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, dimalate Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl Ester type, such as hydroxy stearate isocetyl, 12-stealoyl hydroxy stearyl stearate, and 12-stealoyl hydroxy stearate isostearyl, etc. are mentioned.

Hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, petrolatum, etc. are mentioned as hydrocarbon-based fats and oils.

Examples of silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, and alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

As animal or vegetable oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, saffron oil, soybean oil, corn oil, rapeseed oil, passerine oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , Cottonseed Oil, Palm Oil, Cucui Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Wormwood Colostrum, Marker Damien Nut Oil, Meadow Home Oil, Egg Yolk Oil, Tallow Oil, Horse Oil, Orange Raffy Oil, Jojoba Oil, Candler and animal or vegetable oils and fats such as wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As water-soluble low molecular weight moisturizers, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n=2 or more), polyglycerol B (polymerization degree n=2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Cholesterol, cholesterol ester, etc. are mentioned as a fat-soluble low molecular weight humectant.

In addition, blending components that may be added other than this are not limited thereto, and any of the above components can be blended within a range that does not impair the object and effect of the present invention, but preferably 0.01 to 5% by weight based on the total weight, more Preferably it is blended in 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the fermented herbal plant as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments and fragrances and and such conventional adjuvants and carriers.

The cosmetic composition includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing It may be a formulation selected from the group consisting of formulations of foam, cleansing lotion, cleansing cream, body lotion or body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

Since the fermented medicinal plant of the present invention has antioxidant and antibacterial functions, it may be usefully used as a cosmetic composition.

1 is a schematic diagram showing a manufacturing process of a cosmetic composition comprising an extract of a variety of natural medicinal plants and a fermented product of the extract.
Figure 2 shows the antibacterial activity results of a cosmetic composition comprising an extract of a variety of natural medicinal plants and a fermented product of the extract.
3 shows the antioxidant activity results of a cosmetic composition comprising an extract of a variety of natural medicinal plants and a fermented product of the extract.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is those well known and commonly used in the art.

<Example 1> Manufacturing process of a cosmetic composition comprising fermented medicinal plants

<1-1> Cleaning

Wash all raw materials.

<1-2> Extraction

All of the washed raw materials except red ginseng are extracted with hot water for 4-6 hours at a temperature of 85-95°C, respectively. First, red ginseng is extracted twice at a temperature of 60~55℃ using alcoholic undiluted solution, and the alcohol is recovered to obtain a primary fat-soluble extract phase without alcohol. At a temperature of 4 to 6 hours, the secondary extract phase is obtained through the hot water extraction process, and the primary and secondary extract phases are mixed.

<1-3> Concentration and dilution

Concentrate or dilute all of the extracted raw materials to 12~15 brix, respectively.

<1-4> First fermentation

After concentrating or diluting all of the raw materials whose sugar content is adjusted into three, the total volume of the extract is 200% or more of the volume, and divide it into a glass container or HDPE container that is easy to open and close.

For each container in which each raw material is separated into three, Lactobacillus casei (Lactobacillus casei) in the first container, Saccharomyces cerevisiae (Saccharomyces cerevisiae ) in the second container, and a mixture of these two strains in the third container is inoculated and fermented for 72 hours at a temperature of 32~34℃. The reason for inoculating and fermenting different strains on the same raw material is that the process of fermenting the raw material is different and the metabolites produced are different.

<1-5> Second fermentation

In each product obtained in the first fermentation of Example 1-4, before fermentation of each product, the same material as the material obtained in the dilution process of Example 1-3 is mixed well with 300 to 500% of the first fermentation product After sealing, it is fermented for 5-7 days at a temperature of 24~26℃. In the second fermentation process, the activity of the strain is weaker than that of the first fermentation, so it takes a lot of fermentation time, but the process is easy and the effect of soft and rich in flavor and taste can be obtained.

<1-6> tablet

The resultant obtained in the second fermentation of Example 1-5 is mixed with the same raw materials and centrifuged to obtain a supernatant. The obtained supernatant is again filtered through a sieve of 0.25 μm.

<1-7> Concentration and sterilization

The filtered and purified liquid phase is vacuum-concentrated to 40brix or higher at a temperature of 40°C or less. Put the concentrated liquid in an airtight container and sterilize for 30~90 minutes at 95℃.

<1-8> Formulation and composition

Each sterilized raw material is mixed according to a predetermined ratio to form a cosmetic composition. However, the compounding ratio can be changed as needed.

medicinal plant name ratio licorice root 31 donkey 28 mulberry root 14 Eoseongcho 12 celestial palace 7 nasturtium 6 long-leaved rhubarb root 5 Mint (flowers, leaves, stems) 3 Cornus fruit 4 sauerkraut root 3 red ginseng 2

<Example 2> Antibacterial and antioxidant activity test analysis

<2-1> Sample preparation

For the sample used for the test, the sample prepared in Example 1 was centrifuged for test analysis, filtered through a 0.45 μm filter, and then either directly or freeze-dried was used.

<2-2> Antibacterial activity analysis

1) Measurement of antibacterial activity

The strains used in the test were 1 Gram(+) strain and 2 Gram(-) strains, a total of 3 strains, Staphylococcusaureus(KCTC3881) as aerobic Gram(+), Escherichia coli(KCTC1039), Pseudomonasaeruginosa(KCTC1039) as aerobic Gram(-). KCTC1637), and fungi that cause candidiasis include Candiaalbicans (KCTC7965), Aspergillus niger (KCTC6910), also known as Heukgeukgyun, was used as a microorganism sold from the Center for Biological Resources (KCTC).

The paper-disc method was used to measure the antibacterial activity of a cosmetic composition comprising an extract of a variety of natural medicinal plants and a fermented product of the extract. Through subculture, a single colony of each strain with good activity is taken and smeared evenly over the entire solid medium. Place the sterilized paper-disc on the smeared plate and take 40-60 μl of each extract. Incubate under optimal conditions (30°C) for each strain. After incubation, check and measure the clear zone.

As a result, a cosmetic composition comprising an extract of a variety of natural medicinal plants and a fermented product of the extract was observed in a clear zone of a wide range (5 mm) in Staphylococcus aureus ( S. aureus ), and E. coli ( E. coli ) A clear zone of 2 mm was also observed in P. aeruginosa and Pseudomonas aeruginosa. On the other hand, a clear clear zone could not be observed in the fungi Candida and Heukgeukgyun, and the antibacterial activity of the G&M sample was superior to that of bacteria rather than fungi.

<2-3> Antioxidant activity analysis

By measuring the ability to remove free radicals that cause skin aging, antioxidant activity was evaluated from the scavenging capacity of DPPH (2,2-diphenyl-1-picrylhydrazyl). . DPPH itself is a substance with very stable free radicals. It is a purple compound that exhibits absorbance at 517 nm. It is quantitatively discolored from purple to yellow by an antioxidant that has radical scavenging activity, so it is easy to measure antioxidant activity. Therefore, it is widely used in the search for antioxidants.

The antioxidant ability of a cosmetic composition containing extracts of various kinds of natural medicinal plants and fermented products of the extracts was measured by DPPH assay. Add 0.1 mL of sample dissolved in ethanol (or distilled water) to 1 mL of DPPH 0.2 mM concentration (ethanol solvent). Leave it in the dark at 24°C for 30 minutes. The concentration of scavenged DPPH is quantified by measuring the absorbance at 517 nm. Free radical scavenging activity is calculated by the following equation. As a positive control sample, vitamin C (ascorbic acid) prepared at 100 μg/mL was used.

A: Absorbance after reaction without adding sample

B: Absorbance after reaction with sample addition

C: Absorbance measured by substituting distilled water for DPPH

As a result, DPPH scavenging activity was confirmed in S1 (53.9%) and S2 (82.4%) of the cosmetic composition containing extracts of various kinds of natural medicinal plants and fermented products of the extracts. was confirmed.

Claims (5)

1) Preparation of medicinal plant extracts by hot water extraction of licorice root, chrysanthemum root, mulberry root, eoseongcho, cheongung, chrysanthemum root, rhubarb root, mint, cornflower root and red ginseng at a temperature of 85 to 95 ° C. for 4 to 6 hours. to do;
2) concentrating the extract of step 1) to 12 to 15 brix;
3) Lactobacillus casei ( Lactobacillus casei ) or Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) Inoculated and primary fermentation for 48 to 72 hours at 32 to 38 ℃;
4) Secondary fermentation of the fermented product of step 3) at 24 to 30° C. for 5 to 10 days;
5) centrifuging the secondary fermentation product of step 4) to obtain a supernatant and filtering;
6) concentrating the filtrate of step 5) at 40 to 50° C. to 35 to 45 brix; and
7) A method for producing a fermented medicinal plant comprising the step of sterilizing the concentrate of step 6) at 90 to 100° C. for 30 to 90 minutes. The method according to claim 1, wherein the medicinal plant extract is based on the total weight of licorice root 20 to 30 parts by weight, Angelica basil 20 to 30 parts by weight, mulberry root 10 to 15 parts by weight, Eoseongcho 10 to 15 parts by weight, Cheongung 5 to 10 A method of producing a fermented medicinal plant that is 5 to 8 parts by weight of orchid plant, 3 to 7 parts by weight of long leaf rhubarb root, 1 to 5 parts by weight of mint, 3 to 7 parts by weight of cornflower fruit, and 1 to 3 parts by weight of red ginseng A fermented medicinal plant prepared by the method of claim 1. An antioxidant and antibacterial cosmetic composition comprising the fermented medicinal plant of claim 3 as an active ingredient. According to claim 4, wherein the cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence , packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions or body cleanser formulations selected from the group consisting of an antioxidant and antibacterial cosmetic composition.

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