KR102001236B1 - Composition for improving microbial flora containing extract of Persicae Semen - Google Patents

Abstract

A composition for improving microflora containing an extract of Angelica gigas Nakai as an active ingredient is disclosed. The composition can inhibit the growth of harmful microorganisms and promote the growth of beneficial bacteria.

Description

TECHNICAL FIELD The present invention relates to a composition for improving a microorganism,

The present invention relates to a composition for improving microflora containing an extract of Angelica keiskei koidz. As an active ingredient.

The human body is an ecosystem that is complex and life-active, and various microorganisms exist in the stomach and skin of the human body. Stomach, and skin are referred to as microbial fungi, and 500 to 1000 different microorganisms are symbiotic to form microbial fungi. Intestinal microorganisms present in the stomach synthesize substances such as vitamins and supply them to the human body, and play a role in inhibiting infection by pathogenic microorganisms entering through contaminated food.

Microorganisms that survive on the surface of the skin affect physiological functions such as resistance to human growth, nutrition, immunity and pathogenicity, and play a role in blocking the invasion of external microorganisms, particularly pathogenic microorganisms. In addition, it is known that the microorganisms that reside in the skin perform pH control and skin barrier function of the skin, and change the lipid content of the skin to affect the skin condition. For example, in atopic patients, the ceramide content of the stratum corneum is lowered to have dry and cracked keratin skin. In these atopic patients, it is known that the skin flora is extremely transformed into Staphylococcus aureus (Non-Patent Document One).

Since microorganisms have microorganisms that have a beneficial effect on the human body, it is necessary not to simply remove (sterilize) microorganisms, but to promote the growth of beneficial bacteria while inhibiting the growth of harmful microorganisms.

The present inventors have completed the present invention as a result of carrying out research for improving microorganism microflora using natural products.

 1. HC Korting et al., Eur J Clin Pharmacol 39 (1), 33-36. 1990.

It is an object of the present invention to provide a composition for improving microflora containing an extract of Angelica gigas Nakai as an active ingredient and a cosmetic composition for skin fungal improvement.

In order to accomplish the above object, one aspect of the present invention provides a composition for improving microflora containing an extract of Angelica gigas Nakai as an active ingredient.

As used herein, the term 'microbial flora' refers to a microbial community comprising all the bacteria, fungi, and other prokaryotes present in the human body, and is inhabited mainly in the stomach and skin. For example, intestinal yuikgyun that live in the stomach has a Lactobacillus bacteria (Lactobacillus), Bifidobacterium (Bifidobacterium), harmful bacteria in the intestines is Clostridium (Clostridium) are known.

As used herein, the term " improvement " refers to a condition in which the composition of microbial fungi changes in a beneficial direction to the human body due to the administration of the composition.

Persicae Semen , which is used as an active ingredient of the composition for improving microflora of the present invention, is a pharmacological agent made of peach tree or acid radish seeds of Rosaceae, and has pharmacological effects such as vasodilating action, anti-inflammatory action and antiallergic action It is known. However, there is no known effect of improving the microflora.

In one embodiment of the present invention, the extract may be prepared according to conventional methods known in the art. For example, a crude extract may be prepared by pulverizing doenne, adding a solvent conventionally used for extraction, and extracting at an appropriate temperature and pressure.

In one embodiment of the invention, the solvent is selected from the group consisting of water, hexane, 1,3-butylene glycol, alcohols having 1 to 4 carbon atoms, ethyl acetate, diethyl ether, dichloromethane, acetone and mixtures thereof .

In one embodiment of the present invention, the solvent may be an alcohol having 1 to 4 carbon atoms, and methanol or ethanol may be used.

On the other hand, the extract of the present invention includes both the extract obtained by the solvent extraction method and the extract obtained through the ordinary purification process. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity) Fractions obtained through various purification methods may also be included in the extract of the present invention. In addition, the extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

In one embodiment of the present invention, the composition for improving microflora may contain 0.005 to 10% by weight, or 1 to 5% by weight of the extract described above relative to the total weight of the composition. Accordingly, the content of the active ingredient can be appropriately controlled.

In one embodiment of the present invention, the composition for improving microorganism fungus can promote the growth of beneficial bacteria while inhibiting the growth of harmful microorganisms. Increasing the proportion of the beneficial bacteria in the microorganism can prevent the infection by the pathogenic microorganism, improve the immunity, improve the skin condition, and improve body odor.

In one embodiment of the invention, the harmful bacteria is a propionyl sludge tumefaciens (Propionibacterium), Streptococcus (Streptococcus), Candida (Candida), Acinetobacter (Acinetobacter), Corynebacterium (Corynebacterium) and Pseudomonas (Pseudomons) But are not limited thereto. In addition, the above-mentioned harmful bacteria may be Propionibacterium acnes or Candida albicans .

In one embodiment of the invention, the harmful bacteria can be Staphylococcus aureus (Staphylococcus aureus, Staphylococcus aureus). Staphylococcus aureus is a gram-positive, tuberous anaerobic bacterium that is generally present in the skin and nasal surface of healthy persons or livestock. S. aureus is known to cause not only food poisoning but also purulent diseases such as skin pustules, ear infections and cystitis.

In one embodiment of the present invention, the beneficial bacteria may be Staphylococcus epidermidis ( S. epidermidis ). Staphylococcus epidermidis is a gram-positive bacterium generally present in the skin and mucous membranes of humans and livestock. Staphylococcus epidermidis produces glycerin to sustain the moisturizing effect of the skin (J. Invest. Dermatol., 1960, 34: 171-174), synthesizes organic acids to keep the skin acidic (Br. J. Dermatol 1986, 80: 279-281), it is known to produce antibiotics and inhibit skin growth of harmful microorganisms such as Staphylococcus aureus (N. Engl. J. Med., 1998, 339: 520- 2010, 465: 346-349; Peptides, 2010, 31: 1661-1668; J. Invest. Dermatol., 2010, 130: 192-200).

Another aspect of the present invention provides a cosmetic composition for improving skin fungi comprising an extract of Angelica keiskei koidz. As an active ingredient.

As used herein, the term 'skin resident flora' refers to a microbial community present in the skin, and it is mainly found on skin surface and pores such as scalp. The skin fungus includes skin benefit bacteria that exhibit a beneficial effect on the skin such as moisturizing the skin, removing active oxygen, and skin harmful bacteria that cause skin troubles such as acne and acne.

In one embodiment of the present invention, the cosmetic composition may be in the form of a gel, an emulsion, a suspension, a microemulsion, a microcapsule, a microgranule, an ionic follicle dispersant, a nonionic type follicle dispersing agent, a cream, a skin, a lotion, a powder, Cones stick, aerosol and mask pack. Examples of the cosmetic composition include basic cosmetics, makeup cosmetics, hair cosmetics, body cosmetics, and the like, and can be appropriately selected according to the purpose.

For example, the cosmetic composition can be formulated into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, But is not limited thereto. More specifically, the present invention relates to a cosmetic composition, which comprises at least one of a softening agent, a nutritional lotion, a lotion, a body lotion, a nutritional cream, a massage cream, a moisturizing cream, a hand cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, (cosmetics) such as oil-in-water (O / W) type and water-in-oil (W / O) type, lipstick, makeup base or foundation, toothpaste or mouthwash Hair cosmetics such as hair tonics, gels or mousse, hair cosmetics such as hair tonic or hair dye, and the like.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol , Benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In one embodiment of the present invention, when the formulation of the cosmetic composition is in the form of a suspension, it is preferable to use, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sor Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracant, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica , Talc or zinc oxide may be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Can additionally include propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

In addition, in the cosmetic composition of each formulation, components other than the above extract may be arbitrarily selected and formulated according to the formulation or purpose of the cosmetic composition. In addition, the compositions of each formulation may contain various bases and additives necessary for formulation of the formulation and may contain various additives such as a nonionic surfactant, a silicone polymer, an extender pigment, a perfume, a preservative Emulsifiers, silicones, alpha-hydroxyesters (such as alpha-hydroxyesters), antioxidants, antioxidants, antioxidants, antioxidants, stabilizers, emollients, a preservative, a surfactant, a filler, a polymer, a propellant, a basicizing agent, an acidifying agent, or a coloring agent.

Another aspect of the present invention provides a detergent composition comprising an extract of Guinea as an active ingredient.

In one embodiment of the present invention, the detergent composition may be selected from the group consisting of shampoos, rinse, hair conditioner, body wash, soap, hand wash, laundry detergent and kitchen detergent. In addition to the extracts described above, various bases and additives may be included depending on the purpose of use.

The doenne extract promotes the growth of beneficial bacteria while inhibiting the growth of harmful microorganisms, and thus can be usefully used for the improvement of microflora and the improvement of skin microflora.

FIG. 1 shows the results of measuring the growth rate changes of Staphylococcus epidermidis and Staphylococcus aureus by treatment with Dane extract.
FIG. 2 shows the growth changes by treatment with methanol extract of Staphylococcus epidermidis and Staphylococcus aureus in a mixed culture.
FIG. 3 shows growth changes by treatment with 95% ethanol extract of Staphylococcus aureus and Staphylococcus epidermidis.
FIG. 4 shows the growth changes according to the treatment concentration of 95% ethanol extract obtained by mixing Staphylococcus aureus and Staphylococcus epidermidis.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example  1: Preparation of Dawin Extract

(5 g) was added 95% ethanol or methanol (50 ml) (drug: solvent ratio 1:10) and shaken in a constant temperature water bath at 50 캜 for 3 hours to obtain a first extract. The primary extract was filtered through a filter paper, and the filtered extract was concentrated and dried to prepare a crude extract. 50 mg of the dried extract was dissolved in 1 ml of each extraction solvent. The solids not dissolved in the solvent were removed by centrifugation, and only the supernatant was used as a sample.

Example  2: Changes of Microorganism Growth by Ethanol Extract

2-1. Microorganisms and culture medium

The skin harmful bacteria Staphylococcus aureus; and using the (Staphylococcus aureus, Staphylococcus aureus, hereinafter abbreviated as S. aureus), the skin yuikgyun is Staphylococcus epidermidis (Staphylococcus epidermidis, Staphylococcus epidermidis; or less S. epidermidis ) was used.

S. aureus KCTC 1916 and S. epidermidis KCTC 1917 were purchased from the BRC (Korea Collection for Type Cultures, Korea). The composition of the medium used for culturing the two microorganisms is shown in Table 1 below.

S. epidermidis
KCTC 1917 NB badge
(100 ml) Nutrient Broth 0.8 g (Agar) 1.5 g water Replenish to a total volume of 100 ml S. aureus
KCTC 1916 TSB badge
(100 ml) Tryptic Soy Broth 3.0 g (Agar) 1.5 g water Replenish to a total volume of 100 ml

2-2. Changes in the Growth of Individual Skin-Residing Microorganisms by Dane Extract

S. aureus strain KCTC 1916 strain was plated on TSB (Tryptic Soy Broth, Becton Dickinson and Company, USA) agar plates and cultured at 37 ° C for 24 hours. Thereafter, a single colony was taken and inoculated into 5 ml of TSB liquid medium and preincubated overnight at 37 < 0 > C and 250 rpm. The pre-cultured S. aureus KCTC 1916 strain was inoculated into 20 ml of TSB liquid medium to have an OD ₆₀₀ of 0.05, and 200 μl of 1% (v / v) methanol extract was added. The control group was supplemented with 1% (v / v) methanol instead of doenne extract. After incubation at 37 ° C and 250 rpm, the absorbance was measured at 600 nm using an Optizen 2120 UV plus spectrophotometer (Mecasys Co., Ltd., Daejeon, Korea) every hour.

S. epidermidis KCTC 1917 Growth was also measured by culturing the stock in the same manner as described above except that the stock was cultured on NB (nutrients broth, Becton Dickinson and Company) agar plates.

As shown in FIG. 1, the growth rate of S. epidermidis was increased and the growth rate of S. aureus was decreased by methanol extract. Specifically, the doubling time of S. epidermidis was reduced by 38.42% in the extract treated group (47.55 min) compared to the control group (77.22 min). On the other hand, the doubling time of S. aureus increased about 32.49% in the extract treated group (46.53 min) compared to the control (35.12 min).

2-3. Growth changes of skin-resident microorganisms by methanol extract

The microorganisms were preincubated in the same manner as in Example 2-2, and S. aureus KCTC 1916 strain and S. epidermidis KCTC 1917 strain pre-cultured were mixedly inoculated into 20 ml of NB liquid medium to have an OD ₆₀₀ of 0.05. (1% (v / v)) of methanol extract was added thereto, and the control group was added with 1% (v / v) methanol and cultured at 37 ° C and 250 rpm for 3 hours. Then, 100 μl of the culture was plated on NB agar plates and cultured at 37 ° C for 24 hours to confirm CFU (colony forming unit).

As a result, as shown in FIG. 2, the growth of S. epidermidis was markedly increased and the growth of S. aureus was slightly decreased by treatment with methanol extract.

2-4. Growth changes of skin-resident microorganisms by ethanol extract of 95% ethanol

The degree of microbial growth was confirmed in the same manner as in 2-3 above, except that the 95% ethanol extract at different concentrations (0 to 0.5 g / L) was treated.

As a result, as shown in FIG. 3, the growth of S. aureus was remarkably decreased by treatment with 95% ethanol extract, and the growth of S. epidermidis was increased.

In addition, 95% ethanol extracts of different concentrations (0 to 0.5 g / L) were treated to confirm microbial growth. As a result, as shown in Fig. 4, the growth of S. epidermidis was increased but the growth of S. aureus was remarkably decreased by the 95% ethanol extract.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (10)

The present invention relates to a composition for improving microflora of skin microorganisms,
Wherein said skin microbial fungus comprises Staphylococcus aureus and Staphylococcus epidermidis ,
Wherein said composition inhibits the growth of Staphylococcus aureus and promotes the growth of Staphylococcus epidermidis to improve skin microflora.
The composition of claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, diethyl ether, dichloromethane, acetone, and mixtures thereof.
The composition of claim 1, wherein the extract is from 0.005 to 10% by weight based on the total weight of the composition.
delete delete delete The composition of claim 1, wherein the composition is a cosmetic composition.

The cosmetic composition according to claim 7, wherein the cosmetic composition is a gel, an emulsion, a suspension, a microemulsion, a microcapsule, a microgranule, an ionic follicle dispersant, a nonionic type follicle dispersant, a cream, a skin, a lotion, a powder, , An aerosol, and a mask pack.

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