KR100824212B1 - Composition for skin protection containing placenta and preparation method thereof - Google Patents

Abstract

A skin moisturizing cosmetic composition comprising placenta is provided to recover damaged skin barrier function and maintain the skin moisturizing power for a long time by stabilizing the placenta with an organogel, thereby protecting skin from various diseases such as atopic dermatitis and dry dermatitis and being effective for preventing skin aging. A skin moisturizing cosmetic composition comprises an aqueous solution of placenta encapsulated into a lecithin gel, wherein 0.0001-20 parts by weight of the placenta is used regarding 100 parts by weight of the total composition. A method for preparing the composition comprises the steps of: (a) after adjusting pH of the aqueous solution of the placenta into 3-4, filtering it to obtain filtrate; and (b) adding the filtrate to a solution comprising phosphatidylcholine and glycol with agitating to prepare the lecithin gel. Further, the composition is used as a form of face lotion, eye cream, massage cream, cleansing cream, cleansing foam, cleansing water, pack or essence.

Description

Composition for skin protection containing placenta and preparation method thereof {Composition for skin protection containing placenta and preparation method}

The present invention relates to a composition for protecting skin containing placenta and a method for manufacturing the same, and more particularly, to recover excellent damaged skin barrier function by collecting the placenta in an organogel and stabilizing the skin. The present invention relates to a composition having a moisturizing ability, to preventing skin diseases, and to preventing skin aging, and a method of manufacturing the same.

Skin is always exposed to the outside environment and is an important organ to protect the body. That is, the skin suppresses the loss of water and electrolytes, provides a normal biochemical metabolic environment, and performs a mechanical function and a barrier function to protect the human body from ultraviolet rays and various microorganisms. However, as you age, your skin may age naturally and wrinkles and elasticity may decrease due to the natural environment, especially photoaging, and it may become rough and dry, causing blemishes, freckles, etc. In addition, severe skin diseases may occur. In order to prevent or improve such aging phenomenon or disease, research and product development has been steadily made for the skin protection composition using vitamins, natural extracts and the like. However, satisfactory research results in terms of safety and stability have not been achieved.

Recently, it has been found that the function of the skin barrier plays an important role in relation to aging of the skin and various skin diseases, and interest in the skin barrier is increasing. The main component of the placenta (Zhahager) consists of dozens of amino acids and various active peptides and hundreds of enzymes that are central to pharmacological activity in addition to the essential amino acid. The efficacy of the placenta, known to the extent of consent, has been shown to enhance immunity, antioxidant, anti-inflammatory, whitening, and skin regeneration (Acta Pharmacol. Sin. 2003, 24 (2), 187-92). In particular, Korean Patent No. 24417 discloses a cosmetic composition exhibiting a skin whitening effect by containing a placental extract and kojic acid or kojic acid derivatives to inhibit tyrosinase involved in the initial stage of melanogenesis. However, until now, the research on the skin protection composition for preventing aging using the placenta has not been known.

On the other hand, the term "organogel" was first used to describe the specific concept of gelation of water-in-oil inverse microemulsions with gelatin solutions (Luisi et al. , Colloid & Polymer Science, 1990, vol. 268, pp. 356-374]. The term has recently been extended to gelled systems, mainly hydrogenated systems, containing two immiscible phases (oil-in-water) stabilized with lecithin enriched with phosphatidylcholine (Williman et al. ., Journal of Pharmaceutical Sciences, 1992, vol. 81, pp. 871- 874 and Schchipunov et al., Colloid Journal, 1995, vol. 57, pp. 556-560)). Since this emulsion has a lamellar phase and is in the form of a gel even in the absence of a gelling agent, the name organogel denotes an emulsion of that form regardless of the meaning of the emulsion, whether W / O or O / W. Subsequently, similar emulsions have been developed, in which water is replaced with polyols, which form glycol / oil emulsions and stabilize with hydrogenated lecithin. Emulsions of this type containing high amounts of polyols reduce the epithelial moisture loss (i.e., TEWL) by the membrane formed by the emulsion on the skin, and by supplying a large amount of hygroscopic polyols while allowing skin moisturization, It is particularly advantageous for imparting excellent moisturizing effect to the skin. However, until now, no technique has been known to capture and stabilize the placenta in organogel.

The present inventors have found that the placenta has an excellent effect during the study on the restoration of function of the damaged skin barrier. Furthermore, the present inventors found that dissolving the placenta in water and wrapping the solution treated with organic acid in an organogel structure maximizes the efficacy when applying the skin and can prepare a convenient skin care composition. It was completed.

Accordingly, an object of the present invention is to provide a skin protection cosmetic composition that can restore the function of the damaged skin barrier using the placenta collected in the organogel, protect the skin from various diseases, and prevent skin aging.

Another object of the present invention is to provide a method for preparing the composition.

First, the present invention relates to a cosmetic composition for skin protection, containing the placenta collected in the organogel. In the composition of the present invention, the organogel is preferably lecithin gel, and it is preferable to contain the placenta at 0.0001 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition. The composition of the present invention may be prepared in the form of softening cream, astringent makeup, nourishing cream, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, pack or essence.

Second, the present invention

1) adding an organic acid to the placental aqueous solution to adjust the pH to 3-4, and then filtrate to obtain a filtrate;

2) adding the filtrate of step 1) to a solution containing oil and glycol in an organic solvent and stirring to prepare an organogel:

It relates to a method of preparing the composition, comprising the step. In the process according to the invention, the oil may be one containing phosphatidylcholine, in particular phosphatidylcholine and medium chain triglycerides, the glycol may be propylene glycol, and the organic solvent may be ethanol.

Hereinafter, the present invention will be described in detail.

In the present invention, the placenta is a commercially available product, for example, prepared in an aqueous solution, and then filtered using an organic acid, for example lactic acid, by adjusting the pH to a range of 3-4. .

Organogel is composed of a water-in-oil (W / O) microemulsion system and is an optimal system for stabilizing a water-soluble substance in a gel. Representative examples thereof include gelatin gel and lecithin gel. In the present invention, in particular, lecithin gel can be used, and the placenta aqueous solution prepared by dissolving the placenta in water and adding an organic acid can be collected in the lecithin gel to maximize its effect. Lecithin gel has a skin-like structure that increases the amount of bound water in the product, has a strong moisturizing effect to continuously supply moisture to the skin for a long time when applying the skin, and to prevent the loss of moisture.

The content of the placenta in the composition of the present invention is preferably 0.0001 to 20 parts by weight, more preferably 0.001 to 10 parts by weight, and most preferably 0.01 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition. This is because it is possible to maximize the desired effect within the content range.

The composition of the present invention

1) adding an organic acid, for example lactic acid, to the placental aqueous solution to adjust the pH to 3-4, followed by filtration to obtain a filtrate;

2) The filtrate of step 1) is added to a solution containing oils such as phosphatidylcholine and medium chain fatty acid triglycerides, and glycols such as propylene glycol in an organic solvent such as ethanol and stirred to prepare an organogel:

It may be prepared by a method comprising the step.

The compositions of the present invention may be prepared in any formulation conventional in the art, and may, for example, be formulated in solutions, suspensions, emulsions, pastes, ointments, gels, creams, lotions, powders, soaps, and the like. It is not limited to these. In addition, the composition of the present invention may be prepared in the form of softening cream, astringent makeup, nutrition cream, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, pack or essence, but is not limited thereto no.

Since the composition according to the present invention contains the placenta that restores impaired skin barrier function, the skin moisturizing ability can be greatly improved, and this effect is effective for the prevention and treatment of atopic dermatitis or dry dermatitis.

Hereinafter, the present invention will be described in more detail with reference to Examples, which are intended to aid the understanding of the present invention but are not intended to limit the scope of the present invention in any way.

Preparation Example: Preparation of Placental Aqueous Solution

2 g of placenta were added to 10 ml of water, and then adjusted to pH 3-4 using lactic acid. The aqueous solution was stirred for 10 minutes and the filtrate was collected through filter paper.

Example Preparation of Lecithin Gel Containing Placenta

To a solution in which 6 g of phosphatidylcholine (Lipoid S100-3), 10 g of ethanol, 10 g of medium chain triglyceride (MCT), and 54.0 g of propylene glycol were added, 20 g of the placental aqueous solution prepared in Preparation Example was added and stirred at room temperature to make lecithin. Gels were prepared.

Formulation Example: Preparation of Cream Containing Lecithin Gel

ingredient Content (% by weight) Example Lecithin Gel 20.0 Camellia oil  3.0 Stearyl alcohol  1.5 Stearic acid  1.5 Glyceryl Stearate  1.5 Liquid paraffin 10.0 Beeswax  2.0 Polysorbate  2.5 Sorbitan  0.5 Squalane  3.0 1,3-BG (1,3-butylene glycol)  3.0 glycerin  5.0 Triethanol amine  0.5 Tocopheryl Acetate  0.5 Mountain Mulberry Extract  2.0 antiseptic Quantity Pigment Quantity Spices Quantity Purified water Up to 100

A cream was prepared according to a conventional method with the ingredients and contents shown in Table 1 above.

Comparative Formulation Example: Preparation of Cream without Lecithin Gel

In Table 1, except for the lecithin gel, a cream was prepared in substantially the same manner as in Formulation Example 1 and the content.

Experimental Example 1: Evaluation of moisturizing effect

The moisturizing power of the composition prepared according to the above formulation example and the comparative formulation example was evaluated as follows.

1) water-holding ability test

In a constant temperature and humidity room with a temperature of 25 ° C. and a relative humidity of 45%, the creams obtained in Formulation Examples and Comparative Formulation Examples were respectively applied in an amount of 0.03 g / 16 cm 2 to the inner half of 30 test subjects, followed by rubbing well, and before and after application. After 1 hour and 2 hours the moisture content (moisturizing) of the skin was measured. As a measuring device, a skin moisture content measuring instrument (corneometer CM820, Khazaka Co., Ltd.) was used to measure the moisturizing power by measuring the electric capacitance of the skin according to the moisture content of the skin.

Electrical conductivity Formulation example Comparative Formulation Example Before application  50 50 1 hour after application 111 76 2 hours after application  96 65

As shown in Table 2, the composition containing the lecithin gel of the present invention was found to exhibit a significantly increased (about 1.5 times) moisturizing effect compared to the composition containing no lecithin gel.

2) Moisture evaporation test

After tape stripping on the upper arm of the arm to weaken the skin's protective film, the creams obtained in Formulation Example and Comparative Formulation Example were prepared in 20 constant temperature and humidity chambers with a temperature of 25 ° C. and a relative humidity of 40%. After coating in an amount of 0.03 g / 16 cm 2 inside the upper arm and rubbed well, using a water evaporation meter (TEWL meter, KOLN Co., Ltd.) was measured 5 times at 1 minute intervals every 8 hours to obtain an average value thereof.

Post kill time (hours) Formulation example (%) Comparative formulation example (%)  0   4.1  4.1  8 -10.5 -5.5 16  -8.8 -1.7 24  -6.7  4.1 32  -4.5  5.3

As shown in Table 3, the composition containing the lecithin gel of the present invention was shown to have a significantly increased water evaporation inhibiting effect compared to the composition containing no lecithin gel.

Experimental Example 2: Measurement of the exfoliation effect

In order to measure the exfoliation effect of the composition according to the present invention, the following experiment was performed.

0.4 ml of a 10% dihydroxy acetone (DHA) solution was placed in a hilltop chamber and attached to the inside of the upper arm of 20 testers for 1 hour and then removed. In order to increase the degree of coloration, after 6 hours, the resultant was treated with DHA solution and colored again. Twenty people were randomly divided into two groups, A and B. Group A was applied a cream agent of the formulation example containing lecithin gel, and group B a cream agent of the comparative formulation example containing no lecithin gel was applied twice a day for 2 weeks, and then the change of the skin color was measured by a chromatometer ( Minolta CR300) was used to measure the change in brightness of color (ΔL). In addition, objective visual observation by a plurality of skilled workers and subjective visual observation by a subject were carried out, and the whitening effect was measured according to the following classification: -3: very worse, -2: worse, -1: slightly worse, 0 : No change, 1: slightly improved, 2: improved, 3: highly improved. The results are shown in Table 4.

Skin color change (ΔL) Objective evaluation of the skilled person Subjective Evaluation of the Subject Subject A B A B A B  One 7.2 4.9 3 One 3 2  2 6.9 5.1 3 2 2 3  3 7.7 4.8 3 3 3 3  4 7.3 4.1 2 2 2 One  5 8.0 4.9 3 2 3 One  6 7.5 5.4 3 2 2 One  7 8.8 5.2 3 One 3 0  8 6.7 4.8 3 3 3 2  9 7.4 5.9 3 2 3 3 10 8.7 5.6 2 One 3 3 Average 7.62 5.07 2.9 1.9 2.7 2.0

As shown in Table 4, the group A coated with the lecithin gel-containing cream of the present invention has excellent keratin exfoliation effect in all three evaluations compared to the group B coated with the cream containing no lecithin gel. It was confirmed that.

According to the present invention, by stabilizing the placenta as an organogel can restore the damaged skin barrier function and maintain the skin moisturizing power for a long time can not only protect the skin from various diseases, including atopic dermatitis, dry dermatitis, but also to prevent skin aging Effective compositions can be provided.

Claims (7)

delete A cosmetic composition for moisturizing skin containing an aqueous solution of placenta collected in lecithin gel. The composition of claim 2, wherein the placenta is used in an amount of 0.0001 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition. The composition according to claim 2, which is prepared in the form of softening cream, converging cream, nourishing cream, eye cream, nourishing cream, massage cream, cleansing cream, cleansing foam, cleansing water, pack or essence. delete 1) the pH of the placental aqueous solution was adjusted to 3-4, followed by filtration to obtain a filtrate; 2) adding the filtrate of step 1) to a solution containing phosphatidylcholine and glycol and stirring to prepare a lecithin gel: Method for producing a composition according to any one of claims 2 to 4, comprising the step. The method of claim 6, wherein the solution further comprises a medium chain fatty acid triglyceride and the glycol is propylene glycol.