KR102006712B1 - Cosmetic Compositions Containing Extract of Deer Antler - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of deer antler. A methylene chloride extract of deer antler has the effect of improving skin moisturizing and restoring damaged skin barrier, and thus can be used in cosmetics for the purpose of moisturizing the skin, anti-aging of the skin, and improving the skin barrier.

Description

Cosmetic composition containing antler extract {Cosmetic Compositions Containing Extract of Deer Antler}

The present invention relates to a cosmetic composition for skin moisturizing, skin aging prevention, skin barrier improvement comprising the antler extract.

The skin is the largest organ in our body, occupying about 16% of our total volume, and is directly in contact with the external environment, acting as a barrier to protect the body from many deadly harmful factors and external stimuli that try to break into the body. The barrier function is to protect against various stimuli from the outside such as chemicals, air pollutants, dry environment, ultraviolet rays, etc. and to prevent excessive release of moisture through the skin. This protection function is provided by the stratum corneum consisting of keratinocytes. It can be maintained only if it is formed normally.

The stratum corneum in the outermost stratum corneum is formed from keratinocytes, and is composed of keratinocytes with differentiation and lipid membranes between them. Keratinocytes are characteristic cells in which basal cells that continuously proliferate in the epidermal layer have risen to the surface of the skin through morphological and functional changes in stages.After a certain period of time, old keratinocytes are eliminated from the skin and new keratinocytes function. This iterative series of changes is called "differentiation of epidermal cells" or "keratosis". In addition, during keratinization, keratinocytes form a stratum corneum while generating natural moisturizing factor and intercellular fat, so that the stratum corneum has a firm and flexible function to retain its function as a skin barrier. The intercellular fat is composed of ceramide, cholesterol and free fatty acids, which comprise about 40-65%, 10% and 25% of the stratum corneum lipids, respectively. In addition, it has a composition in which a small amount of phytosphingosine and sphingosine are present together.

As the body ages, the amount of collagen, mucopolysaccharides and hyaluronic acid in the dermis matrix, which play an important role in maintaining skin elasticity, decreases and the amount of insoluble collagen increases, making the dermis difficult to maintain moisture in the skin and making the skin rough. do. In addition, as skin aging progresses, cell division does not proceed well, and thus, the keratinization cycle is delayed and becomes uneven, thereby deteriorating the skin barrier function of the stratum corneum. In addition, the function of sebaceous glands and sweat glands is reduced, and the amount of sebum and sweat is gradually reduced, and the sebum membranes are not properly produced, and thus the protection for skin moisturization is lost, resulting in dry skin and loss of transparency. Elasticity is reduced and wrinkles are created. In other words, the loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related.

In addition to aging, the stratum corneum can easily lose its function due to lifestyle factors such as excessive washing or bathing, environmental factors such as dry air pollutants, and diseases such as atopic skin. Recently, due to the increased variety of factors, the symptoms of dry skin and the resulting symptoms are gradually increasing.

In order to suppress the skin aging or to slow the progression of the skin, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoids, tocopherol have been developed, but such cosmetics or skin protectants In the final formulation, the potency is low, the efficacy is mild, and the skin irritation has a serious problem.

Recently, cosmetic products using natural vegetable raw materials, which are more safe and irritating to the skin due to the skin irritation problem of these raw materials, have been developed. In particular, in Korea, many cosmetic products using natural herbal ingredients have been developed. This is because natural herbal ingredients have been used in private for a long time and safety and effectiveness have already been secured. Because there is. As a raw material of cosmetics, the situation where plants are generally studied, but raw materials derived from animals have not been studied.

Deer antler is a dry horn taken from a freshly grown horn of a deer. In mid-March each year, deer lose their old horns and new horns start to sprout. At this time, young horns that are not ossified are called antlers. Ganglioside, a type of glycolipid among the fat-soluble components of antler, is a glycolipid of polymers accumulated in the gray matter of kidney, liver, spleen and brain, and clinical trials have shown that it is effective for diabetes and neurotoxicity related to neurotransmission disorders. It is known (patent document 1).

As can be seen from the results of the animal and clinical experiments as described above, antler is an animal herbal medicine of high value as a therapeutic agent containing a large amount of various components effective for the human body. The present inventors studied whether the extract derived from the antler has an effect of enhancing the skin moisturizing ability and improving the damage of the skin barrier in addition to the known physiological effects of the antler, and the antler is described as methylene chloride (hereinafter referred to as MC). The present invention was completed by confirming that the extract obtained by extracting with a solvent was effective in improving the moisturizing power of the skin and improving the damaged skin barrier as compared with the extract prepared by extracting with another solvent.

Korean Unexamined Patent Publication No. 1994-0013381

In order to solve the problem that the skin protection agent, which has been used to suppress skin aging or to slow the progression, has a slight effect and severe skin irritation, it enhances the skin's moisturizing power among natural herbal ingredients, which have secured stability and effectiveness, We investigated raw materials that could improve the damage to the barrier and alleviate the symptoms associated with skin barrier damage.

In order to solve the above problems, the present invention provides a skin moisturizing cosmetic composition comprising a methylene chloride extract of antler.

The present invention provides a cosmetic composition for preventing skin aging comprising methylene chloride extract of antler.

The present invention provides a cosmetic composition for improving skin barriers comprising methylene chloride extract of antler.

The methylene chloride extract of the antler of the present invention has an excellent skin moisturizing effect, and has an effect of restoring a damaged skin barrier, thereby improving reduction of skin elasticity and wrinkle formation by lowering skin moisturizing ability, and drying of skin due to skin barrier damage or Symptoms such as dermatitis can be improved.

1 shows a method for preparing MC extract of antler origin.
Figure 2 shows a method of producing MC extract of the antler residue, ethanol extract of the antler residue, water extract of the antler residue.
Figure 3 shows the skin moisturizing effect of MC extract of the antler extract, MC extract of the antler residue.
4 shows the skin barrier improvement effect of the MC extract of the antler extract, MC extract of the antler residue.

Hereinafter, the present invention will be described in detail.

The present invention provides a skin moisturizing cosmetic composition comprising an antler methylene chloride extract, a skin anti-aging cosmetic composition comprising an antler methylene chloride extract, or a cosmetic composition for improving a skin barrier comprising an methylene chloride extract of an antler.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material may be used without limitation to those raised or commercially available.

When the extract is obtained by extracting it from raw materials, all conventionally known extraction methods such as solvent extraction, ultrasonic extraction, filtration and reflux extraction can be used as extraction methods, and preferably prepared by using solvent extraction or reflux extraction. can do. The extraction process may be repeated several times, after which it may be further subjected to steps such as concentration or lyophilization. Specifically, the obtained extract may be concentrated under reduced pressure to obtain a concentrate, and the extract may be lyophilized to prepare a high concentration of extract powder using a grinder.

The antler may be an unprocessed deer antler raw material (hereinafter, referred to as 'an antler raw material'), or may be an antler residue (hereinafter, referred to as 'an antler residue') except liquid after extracting an antler raw material with an extraction solvent. .

The extraction solvent is water, C1-C4 alcohol, n-hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene and a mixed solvent thereof It may be any one selected from, and preferably the solvent may be any one selected from the group consisting of water, a C1-C4 alcohol and a mixed solvent of water, more preferably ethanol or an aqueous ethanol solution.

The ethanol aqueous solution may be 20% (v / v) to 55% (v / v) aqueous solution of ethanol, preferably 25% (v / v) to 50% (v / v) aqueous solution of ethanol, More preferably, the aqueous solution of ethanol may be 35% (v / v) to 45% (v / v), but is not limited thereto. When the ethanol in the ethanol aqueous solution is contained below the lower limit, there is a problem that the active ingredient extraction of the antler is inhibited, and when the ethanol in the ethanol aqueous solution is contained above the upper limit, the MC extract of the residue obtained after the extraction of the antler ethanol finally obtained The skin moisturizing effect or the skin barrier improving effect may be inhibited.

In one embodiment of the present invention, it was confirmed that the MC extract of the antler origin obtained by extracting the antler origin with methylene chloride has a skin moisturizing effect and improves the damaged skin barrier (FIG. 3).

In another embodiment of the present invention, it was confirmed that the MC extract of the antler residue obtained by primary extraction of the antler with ethanol extracted methylene chloride has a skin moisturizing effect and improves the damaged skin barrier (FIG. 4). .

The active extract of the MC extract of the antler extract or the MC extract of the antler residue is separated from the extract containing a high concentration of the active ingredient having a skin moisturizing effect or a skin barrier improvement effect, also called an active fraction or an active fraction. . Particularly active fractions having higher active ingredients can be prepared by separating them according to the nature of the active ingredient through concentration gradient column chromatography among the fractions obtained by the usual fractionation process such as systematic fractionation. have. The column chromatography performs column chromatography using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl and XAD resin to separate active fractions. And may be purified, and column chromatography may be performed several times by selecting an appropriate filler as necessary, but is not limited thereto. In using the chromatography, an eluting solvent, an eluting rate, and an eluting time may apply a solvent, a rate, or a time generally used in the art.

The amount of methylene chloride extract of the antler of the present invention may be included in the cosmetic composition in an amount of 0.01 to 30% by weight, preferably 0.01 to 20% by weight, more preferably 0.01 to 10% by weight, Even more preferably, it may be included in 0.01 to 5% by weight. If the content is less than 0.01% by weight, the effect of the antler methylene chloride extract may be insignificant. If it exceeds 30% by weight, the effect of the antler methylene chloride extract relative to the input concentration may be relatively low.

In one embodiment of the present invention, the MC extract of the antler extract and the MC extract of the antler residue are added to the cosmetic formulation at a final concentration of 5% by weight, and when applied to the skin, the moisture of the skin is maintained as compared with the extraction with other solvents. The improvement rate of skin moisturizing was high compared with glycerol which is a positive control group. In addition, in terms of skin barrier effect, MC extract of the antler extract and MC extract of the antler residue had a higher skin barrier recovery rate than the other extracts.

As described above, the antler methylene chloride extract of the present invention has an effect of improving skin moisturizing, and thus may be used to enhance skin moisturizing power. In addition, it is used to prevent skin aging because the skin moisturizing is increased by increasing skin elasticity and skin wrinkles are reduced. In addition, the methylene chloride extract of the antler of the present invention is damaged and has an effect of restoring the skin barrier, thereby preventing or improving skin dryness, atopic dermatitis, contact dermatitis or psoriasis, etc., caused by incomplete function of the skin barrier.

In the cosmetic composition of the present invention, the fraction may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form may include, but are not limited to, for example, cosmetics, creams, lotions, baths and the like.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited and include, for example, water, alcohols, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin and the like.

When the composition of the present invention is made into a cosmetic composition, the composition of the present invention may include not only methylene chloride extract of antler, but also components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins , Conventional adjuvants such as pigments and perfumes, and carriers.

Cosmetic compositions of the invention can be prepared in any formulations conventionally prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin moisturizing effect and skin barrier improvement effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the present invention will be described in detail by the experimental and production examples.

However, the following Experimental Examples and Preparation Examples are only for illustrating the present invention, and the content of the present invention is not limited by the following Experimental Examples and Preparation Examples.

Preparation Example 1 Preparation of Deer Antler Methylene Chloride (hereinafter referred to as MC) Extract

The antler was dried and carved out. The antler was added with 8 times the MC to the weight of the antler, and was first cooled for 24 hours. After 24 hours, the primary MC extract was recovered, and the remaining residue was added with 8 times the number of MC in the same manner, followed by secondary cooling for 24 hours. After mixing the primary and secondary extracts, the solvent was removed to obtain 10 g of the dried product of the extract. The manufacturing method of MC extract of a deer antler is shown in FIG.

Preparation Example 2 Preparation of Methylene Chloride, Ethanol, and Water Extract of Residue After Extraction of Deer Antler Ethanol

The antler was dried and carved out. The antler was immersed in 35% ethanol at 5.8 times the weight of the antler for 3 days. After recovering only the residue from the extract and hot-air dried for 2 days, MC, ethanol, distilled water was added to the dried residue 8 times the weight of the dried residue, respectively, and was first cooled for 24 hours. The first extract was recovered and secondly soaked for 24 hours for each solvent in the same manner as the first extraction. After mixing the primary and secondary extracts, the solvent was removed to obtain 10 g of dried MC extract of the antler residue, 100 g of dried ethanol extract of the antler residue, and 114 g of dried water extract of the antler residue. The extract manufacturing method of the antler residue is shown in FIG.

Example 1 Checking skin moisturizing power

In the experimental group, the MC extract of the antler extract obtained in Preparation Examples 1 and 2, the MC extract of the antler residue obtained by extracting the residue remaining after the extraction of the antler ethanol with MC, ethanol and distilled water, the ethanol extract of the antler residue, and the antler residue Water extract of was used as a sample. Glycerol was used as a positive control. Each sample was dissolved in a cosmetic formulation (vehicle) shown in Table 1 to a final 5% concentration.

function Raw material name content
((w / w)%) Paid




Emulsifier Cetearyl alcohol 2.0 Glyceryl stearate 0.4 Sorbitan stearate 0.4 oil

Squalene 4.0 Caprylic / capric triglyceride 2.0 Dimethicone 0.4 Awards





Purified water Water to 100 Moisturizer Glycerin 6.0 antiseptic
Caprylyl glycol 0.3 Ethyl hexanediol 0.2 Polymer Polyacrylate crosspolymer-6 1.0 chief ingredient Extract of Preparation Examples 1 and 2 5.0 Spices incense 0.1

The forearm of the subject was washed with running water, and a total of seven test sites were marked by a square of 2 × 2 cm. The test site was an uncoated area, MC extract of the antler source of Preparation Example 1, MC extract of the antler residue of Preparation Example 2, which is a residue extract of antler ethanol extract, ethanol extract of the antler residue, water extract of the antler residue, and positive The control application site, the cosmetic formulation except for the main component in Table 1, the application site, and was labeled to be divided by test sites. Prior to the application of cosmetics, the moisture content of the skin before application of cosmetics at each test site was measured by Corneometer CM825. The prepared cosmetics were evenly applied to each test site by 2 μl / cm 2, and the water content was measured by Corneometer CM825 at 1 hour intervals after application. The moisture content after application was calculated based on the moisture content 100 of each test site before application.

Moisture content after the application of cosmetic formulations was higher than that of MC extract of the antler extract of Preparation Example 1 and MC extract of the antler residue of Preparation Example 2, compared to ethanol extract of the antler residue of Preparation Example 2 and water extract of the antler residue. Is shown (FIG. 3). In addition, there was no difference in the moisturizing effect between the MC extract of the antler extract and the MC extract of the antler residue, and both extracts obtained using MC as a solvent showed an equivalent or superior moisturizing improvement effect compared to glycerol used as a positive control group.

Example 2 Confirmation of Skin Barrier Effect

After washing the forearm of the subject with running water, five test sites (no coating site, MC extract of antler residue, MC extract of antler residue, ethanol extract of antler residue) using a fin chamber were used. 20 μl of 1% sodium lauryl sulfate was closed closed for 24 hours to damage the skin barrier at the application site of the water extract of the antler residue. After 24 hours, the pin chamber was removed, the test sites were rinsed with water, dried, and the Tewameter TM300 was used to measure the transdermal water loss (TEWL: Trans Epidermal Water Loss, also referred to as 'epidermal moisture loss'). The transdermal moisture loss means that water supplied from the dermis of the skin is mainly diffused to the upper part of the stratum corneum by passive diffusion and lost to the outside. Cosmetics containing each sample prepared in Example 1 were applied to each test site twice daily and evening. Percutaneous moisture loss was measured at 4 days, 7 days, 9 days and 15 days after application. The skin barrier recovery rate was calculated by setting the transdermal moisture loss of the test site to 100 after the skin barrier injury.

As a result of the experiment, the moisture content after applying the cosmetic formulation was higher than that of MC extract of Deer Antler in Preparation Example 1 and MC extract of Deer Antler residue in Preparation Example 2, compared to ethanol extract of Deer Antler residue in Preparation Example 2 and water extract of Deer Antler residue. It was confirmed that the skin barrier improvement effect was excellent, in particular, the degree of skin barrier recovery was significantly higher than other samples at the beginning of application after 4 days of application (FIG. 4). In addition, there was no difference in the skin barrier improvement effect between the MC extract of the antler extract and the MC extract of the antler residue.

Preparation Example 3 Preparation of Cosmetic Composition

<2-1> Preparation of Essence

Essence was prepared according to the content (parts by weight) shown in Table 2 below using the MC extract of the antler raw material of Preparation Example 1.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 MC extract of the antler origin of Preparation Example 1 1.5 Wax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Suitable amount

<2-2> Preparation of Flexible Cosmetic Water

Softening water containing MC extract of the antler residue of Preparation Example 2 as an active ingredient was prepared as shown in Table 3.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated Castor Oil 1.00 ethanol 30.00 MC extract of the antler residue of Preparation Example 2 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<2-3> Preparation of nutrition cream

Nutritional cream containing MC extract of the antler raw material of Preparation Example 1 was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 MC extract of the antler origin of Preparation Example 1 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Flavoring agent Suitable amount Purified water Balance

<2-4> Preparation of Lotion

A lotion containing MC extract of the antler residue of Preparation Example 2 as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Pro-type glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbic acid sesquioleate 0.6 Squalene 4.8 Macadia oil 2 Jojoba oil 2 Tocopherol Acetate 0.4 Methylpolysiloxane 0.2 Tocopherol Acetate 0.4 1,3-butylene glycol 4 Xanthan gum 0.1 glycerin 4 d-Pandenol 0.15 MC extract of the antler residue of Preparation Example 2 1.0 Allantoin 0.1 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Suitable amount

Claims (12)

A cosmetic composition for moisturizing skin comprising methylene chloride extract of antler,
The antler is an antler antler, or a skin moisturizing cosmetic composition which is an antler residue except for liquid after extracting the antler ant from the extraction solvent. delete The method according to claim 1,
The extraction solvents used in the preparation of the antler residues are water, C1-C4 alcohol, n-hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene and Cosmetic composition for skin moisturizing which is any one selected from the group consisting of these mixed solvents. The method according to claim 3,
The extraction solvent is ethanol or ethanol aqueous solution for skin moisturizing cosmetic composition. As a cosmetic composition for preventing skin aging by improving skin moisturizing containing methylene chloride extract of antler,
The antler is an antler antler, or a skin composition for preventing skin aging by extracting an antler antler with an extraction solvent and improving the skin moisturizing residue of the antler except for liquid. delete The method according to claim 5,
The extraction solvents used in the preparation of the antler residues are water, C1-C4 alcohol, n-hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene and Cosmetic composition for preventing skin aging by improving skin moisturizing which is any one selected from the group consisting of these mixed solvents. The method of claim 7,
The extraction solvent is a cosmetic composition for preventing skin aging by improving skin moisturizing ethanol or aqueous ethanol solution. As a cosmetic composition for improving the skin barrier comprising methylene chloride extract of antler,
The antler is an antler antagonist, or after extracting the antler antler with an extraction solvent, a cosmetic composition for improving skin barrier, which is a antler residue except liquid. delete The method of claim 9,
The extraction solvents used in the preparation of the antler residues are water, C1-C4 alcohol, n-hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene and Cosmetic composition for skin barrier improvement which is any one selected from the group consisting of these mixed solvents. The method of claim 11,
The extraction solvent is a cosmetic composition for improving skin barrier ethanol or ethanol aqueous solution.

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