JP2015086183A - Composition for skin photoaging prevention - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition that has skin aging prevention effect, which has been impossible in conventional compositions.SOLUTION: This invention provides a composition that inhibits MMP-1 expression enhancement due to ultraviolet damage and also inhibits MMP-1 expression enhancement due to damage caused by near infrared rays.

Description

  The present invention relates to a composition for preventing skin aging, which prevents skin aging caused by light irradiation.

Light irradiation is known as one of the causes of skin aging.
It is said that ultraviolet rays A (UVA), ultraviolet rays B (UVB), and near-infrared rays are mixed with light of various wavelengths.
It is widely known that UVA (wavelength: 320 nm to 400 nm) and UVB (wavelength: 280 nm to 320 nm), which have shorter wavelengths than visible light, have high energy and adversely affect the skin.
It is also known that near infrared rays (wavelengths of 780 nm to 3000 nm) also have an adverse effect on the skin.

  Patent Document 1 discloses a cosmetic composition capable of simultaneously blocking ultraviolet rays and near infrared rays that adversely affect the skin. Specifically, at least one selected from the group consisting of boron nitride for blocking near infrared rays and cerium oxide, titanium oxide, talc, aluminum oxide, iron oxide, zinc oxide and mica for blocking ultraviolet rays A cosmetic composition comprising an inorganic powder is disclosed.

On the other hand, it is known that the expression of MMP-1 (collagen degrading enzyme) is increased by ultraviolet rays, and wrinkles are formed or elasticity is lowered, and skin aging is maintained without wrinkles and sagging. Plant extracts having an MMP-1 inhibitory action are known as anti-aging agents that have an action to prevent the above (see Patent Document 2).
Patent Document 1 describes that wrinkles are formed by increasing the expression of MMP-1 even by irradiation with near infrared rays.

Special table 2013-511515 gazette JP 2000-154132 A

  This invention makes it a subject to provide the new composition which prevents the aging of the skin by the damage of an ultraviolet-ray and a near-infrared ray.

In order to provide the above-mentioned new composition, the present inventors have paid attention to MMP-1 expression and repeated research. As a result, the present inventors have found a new finding that the expression enhancement of MMP-1 differs between ultraviolet irradiation and near infrared irradiation, and there is a large time lag in the expression enhancement of MMP-1.
The present inventors have further studied based on the new findings, and not only suppress the increase in MMP-1 expression due to ultraviolet rays, but also suppress the increase in MMP-1 expression due to near infrared rays, thereby preventing skin aging. It was conceived that a composition that can make a great contribution to the above can be provided.

  The present invention is a composition for preventing skin aging, comprising an ultraviolet damage inhibitor and a near infrared damage inhibitor.

The near-infrared damage inhibitor is preferably a sustained release carrier containing an MMP-1 inhibitor.
Moreover, it is preferable that the said near-infrared damage inhibitor contains 1 or more types selected from the group which consists of a peony extract, a sage extract, a chamomile extract, a geno shochu extract, a cypress extract, a pomegranate extract, and an altea extract.
In addition, the above-mentioned UV damage inhibitor includes loquat extract, carrot extract, ginseng extract, neem extract, assembly extract, rosemary extract, clove extract, horsetail extract, clove extract, loofah extract, mugwort extract, carambola extract, assembly extract, lotus root It is preferable to include one or more selected from the group consisting of an extract, an aurene extract, an arnica extract, a melissa extract, a clara extract, a yarrow extract, a ginger extract, and a hypericum extract.

The composition for preventing skin aging according to the present invention is based on a new finding that the expression state of MMP-1 differs between ultraviolet irradiation and near infrared irradiation, and has a conventional MMP-1 inhibitory action. Compared to a composition simply containing an extract, it has a remarkable anti-aging effect on skin.
Therefore, the composition for preventing skin aging according to the present invention is preferably used as an external preparation for skin, and more preferably used as a cosmetic. Moreover, since it does not absorb ultraviolet rays and near infrared rays but suppresses damage caused by ultraviolet rays and near infrared rays, it can also be used as food.

It is a graph which shows MMP-1 mRNA expression level by UVA irradiation as MMP-1 mRNA expression level of a non-irradiation group as 1 (irradiation amount: 25 J / cm < ² >). It is a graph which shows the MMP-1 mRNA expression level by near-infrared irradiation as MMP-1 mRNA expression level of a non-irradiation group as 1 (irradiation amount: 400 J / cm < ² >). It is a figure which shows the morphological change of the cell by near infrared irradiation (drawing substitute photograph).

  Hereinafter, the composition for preventing skin aging of the present invention will be described, but the technical scope of the present invention is not limited to the following specific embodiments.

The composition for preventing skin aging according to the present invention is a composition for preventing skin aging comprising an ultraviolet damage inhibitor and a near infrared damage inhibitor.
The present invention is based on the new finding that the expression enhancement of MMP-1 differs between ultraviolet irradiation and near infrared irradiation, and there is a large time lag in the expression enhancement of MMP-1.
The present inventors have obtained such new knowledge through experiments described below.

<Experimental example 1: MMP-1 expression enhancement by UVA irradiation>
Fibroblasts were irradiated with UVA (wavelength 350 to 390 nm) so that the irradiation dose was 25 J / cm ² .
After irradiation with UVA, fibroblasts were recovered 24 hours, 48 hours, and 72 hours, respectively, and MMP-1 mRNA expression analysis was performed. MMP-1 mRNA expression analysis is StepOne Real
It was performed by Time PCR System (Applied Biosystems).
The MMP-1 mRNA expression level in the UVA non-irradiated group is 1, and the MMP-1 mRNA expression level in the cells 24 hours, 48 hours, and 72 hours after irradiation is shown in FIG.

<Experimental example 2: MMP-1 expression enhancement by near infrared irradiation>
Fibroblasts were irradiated with near-infrared rays (wavelength 700-1400 nm) at an irradiation dose of 400 J / cm ² .
After irradiation with near infrared rays, fibroblasts were collected 24 hours, 48 hours, and 72 hours later, respectively, and MMP-1 mRNA expression analysis was performed. MMP-1 mRNA expression analysis was performed by StepOne Real Time PCR System (Applied Biosystems).
FIG. 2 (A) shows the MMP-1 mRNA expression level in the cells 24 hours, 48 hours, and 72 hours after irradiation, assuming that the MIR-1 mRNA expression level in the near-infrared non-irradiated group is 1.
In addition, peony extract was added to fibroblasts immediately after near infrared irradiation, and the expression level of MMP-1 mRNA in the cells after 72 hours is shown in FIG. 2 (B).

<Experimental Example 3: Morphological Deterioration of Fibroblasts by Near-Infrared Irradiation>
In the said Experimental example 2, when the fibroblast after near infrared irradiation was observed with the phase-contrast microscope, cell extension was suppressed by near infrared irradiation as shown in FIG.3 (B). However, when peony extract was added to fibroblasts immediately after near-infrared irradiation, no morphological deterioration of fibroblasts was observed as shown in FIG.
On the other hand, the morphological deterioration of the fibroblasts was not confirmed in the UVA irradiation group.

In the UVA irradiation group, increased expression of MMP-1 was observed early after irradiation, and in the near infrared irradiation group, increased expression of MMP-1 was observed after a certain period after irradiation.
Based on such knowledge, in order to suppress skin aging based on damage to the skin caused by light irradiation, the present inventors have caused damage caused by ultraviolet rays, that is, increased expression of MMP-1 observed at an early stage after light irradiation. And the present inventors have found that it is important to inhibit the damage caused by near infrared rays, that is, the enhancement of MMP-1 expression observed after a certain period of time has elapsed after light irradiation.

<UV damage inhibitor>
A UV damage inhibitor refers to a component that can suppress the increase in MMP-1 expression caused by UV irradiation, which occurs at an early stage after light irradiation.
It has been clarified by the present inventors that MMP-1 expression enhancement by ultraviolet rays occurs at an early stage after light irradiation. Damage due to ultraviolet rays refers to increased expression of MMP-1 occurring after 10 minutes after ultraviolet irradiation, may be increased expression of MMP-1 occurring after 30 minutes, and increased expression of MMP-1 occurring after 45 minutes It may be the expression enhancement of MMP-1 occurring after 1 hour. On the other hand, it refers to the increased expression of MMP-1 before the lapse of 42 hours, may be the increased expression of MMP-1 before the lapse of 36 hours, or may be the increased expression of MMP-1 before the lapse of 30 hours MMP-1 expression may be increased before 24 hours.

Judgment whether or not it is an ultraviolet damage inhibitor can be performed as follows.
A cell to which the test substance is added and a control cell to which the test substance is not added are prepared and irradiated with ultraviolet rays. When MMP-1 expression enhancement or MMP-1 mRNA expression enhancement is suppressed by 1% or more at 24 hours after ultraviolet irradiation, it can be regarded as an ultraviolet damage inhibitor and suppressed by 3% or more. It is preferably 5% or more, more preferably 10% or more, more preferably 20% or more, particularly preferably 30% or more. Particularly preferred.

As the ultraviolet damage inhibitor, known ultraviolet damage inhibitors can be used, and various plant extracts and the like can be used.
Specific examples of such plant extracts include loquat extract, carrot extract, ginseng extract, neem extract, assembly extract, rosemary extract, clove extract, horsetail extract, clove extract, loofah extract, mugwort extract, carambola extract, assembly extract , Astragalus extract, Aurelia extract, Arnica extract, Melissa extract, Clara extract, Achillea millefolium extract, Ginger extract and Hypericum extract.
The ultraviolet damage inhibitor is usually contained in the composition of the present invention in an amount of 0.001% by mass or more, preferably 0.01% by mass or more, more preferably 0.1% by mass or more, and usually 30% by mass or less, preferably Is 20% by mass or less, more preferably 10% by mass or less.
In addition, only one type of ultraviolet damage inhibitor may be contained, or two or more types may be contained.

<Near-infrared damage inhibitor>
A near-infrared damage inhibitor refers to a component that can suppress the increase in MMP-1 expression caused by irradiation with near-infrared light that occurs after a certain period of time has elapsed after light irradiation.
It has been clarified by the present inventors that MMP-1 expression enhancement by near-infrared light occurs after a certain period after light irradiation. The near-infrared damage refers to an increase in the expression of MMP-1 that usually occurs after 42 hours from the irradiation of the near-infrared ray, and may be an increase in the expression of MMP-1 that occurs after 48 hours. May be increased expression of. On the other hand, it may be an increase in the expression of MMP-1 before 90 hours, an increase in the expression of MMP-1 before 84 hours, or an increase in the expression of MMP-1 before 78 hours. It may be an increase in the expression of MMP-1 before 72 hours.

Judgment whether it is a near-infrared damage inhibitor can be performed as follows.
A cell to which the test substance is added and a control cell to which the test substance is not added are prepared and irradiated with near infrared rays. When the increase in MMP-1 expression or the increase in MMP-1 mRNA expression is suppressed by 1% or more at 72 hours after the near infrared irradiation, it can be regarded as a near-infrared damage inhibitor and can be suppressed by 3% or more. Is preferably suppressed by 5% or more, more preferably is suppressed by 10% or more, particularly preferably is suppressed by 20% or more, and is suppressed by 30% or more. It is particularly more preferable that 50% or more is suppressed.

The near-infrared damage inhibitor is not particularly limited as long as it suppresses the increase in MMP-1 expression after a certain period of time has passed since near-infrared irradiation. For example, an MMP-1 expression inhibitor such as the aforementioned ultraviolet damage inhibitor can be controlled. It may be included in the carrier.
The sustained-release carrier may be any one that can release the contained MMP-1 expression inhibitor with delay, and is usually 24 hours after administration, preferably 30 hours after administration, more preferably 36 hours after administration. It is sufficient that the contained MMP-1 expression inhibitor can be released until 42 hours after administration, more preferably 48 hours after administration.

  As the sustained-release carrier, a sustained-release carrier used for an external preparation for skin and a sustained-release carrier used for food are appropriately used. Specific examples include liposomes, niosomes, microcapsules and the like.

Moreover, as a near-infrared damage inhibitor, various plant extracts etc. which can suppress near-infrared damage can be used.
Specific examples include peony extract, sage extract, chamomile extract, genus shochu extract, saxifrage extract, pomegranate extract, and altea extract.
The near-infrared damage inhibitor is usually contained in the composition of the present invention in an amount of 0.001% by mass or more, preferably 0.01% by mass or more, more preferably 0.1% by mass or more, and usually 30% by mass or less. Preferably it is 20 mass% or less, More preferably, it is 10 mass% or less.
In addition, only 1 type may be contained for a near-infrared damage inhibitor, and 2 or more types may be contained.

  The content ratio (mass ratio) in the composition of the ultraviolet damage inhibitor and the near infrared damage inhibitor is not particularly limited, but the ultraviolet damage inhibitor: near infrared damage inhibitor = 1: 100 to 100: 1. What is necessary is just to be 1: 10-10: 1.

The composition for preventing skin aging can be applied to cosmetics, quasi-drugs, skin external preparations including pharmaceuticals, and foods, and can contain necessary components as appropriate according to each application. .
Among these, it is preferable to apply to an external preparation for skin, particularly cosmetics.
When applied to cosmetics, it is possible to broadly blend the components normally used in cosmetics, and the dosage form and use are not limited at all. Hereinafter, the components that can be contained in the cosmetic when applied to the cosmetic will be described.

Examples of active ingredients include whitening ingredients, anti-inflammatory ingredients, plant extracts and the like. In addition, you may overlap with the ultraviolet-ray damage inhibitor and near-infrared damage inhibitor demonstrated above.
The whitening component is not particularly limited as long as it is generally used in cosmetics. For example, 4-n-butylresorcinol, ascorbic acid glucoside, 3-O-ethylascorbic acid, tranexamic acid, arbutin, 2-[(triphenylmethyl) oxy] ethanol, 1- (triphenylmethyl) piperidine, N -(P-toluyl) cysteic acid,
N- (p-methoxybenzoyl) cysteic acid and the like can be mentioned. Some of these whitening components are already on the market, or they can be obtained by synthesis. For example, 3-O-ethylascorbic acid can be synthesized by a known method described in JP-A-8-134055. There are also commercially available products (“VC ethyl” manufactured by Nippon Seika Co., Ltd.), and these can be obtained and used. 2-[(Triphenylmethyl) oxy] ethanol and 1- (triphenylmethyl) piperidine are disclosed in Patent Document WO 2010-074052 as N- (p-toluyl) cysteic acid and N- (p-methoxybenzoyl). Cysteinic acid can be synthesized according to the disclosure since its synthesis method is disclosed in WO2010-058730.
Content of the whitening component in cosmetics is 0.01-30 mass% normally, 0.1-10 mass% is preferable and 1-5 mass% is more preferable.

The plant extract is not particularly limited as long as it is generally used for pharmaceuticals, cosmetics and the like. For example, akebi extract, asunaro extract, asparagus extract, avocado extract, achacha extract, almond extract, arnica extract, aloe extract, apricot extract, ginkgo biloba extract, fennel extract, ages extract, enmiso extract, oxon extract, agar extract, auren Extract, ginseng extract, hypericum extract, nettle extract, orange extract, oyster extract, cuckoo extract, chamomile extract, carrot extract, kawara mugi extract, licorice extract, kiwi extract, cucumber extract, guava extract, cucumber extract, gardenia extract, kumazasa extract, clara Extract, walnut extract, grapefruit extract, black rice extract, chlorella extract, mulberry extract, ghetto extract, gentian Kiss, Genno pepper extract, Black tea extract, Burdock extract, Rice extract, Rice fermented extract, Rice bran fermented extract, Rice germ oil, Cowberry extract, Salvia extract, Soap extract, Sasa extract, Hawthorn extract, Sansha extract, Salamander extract, Shiitake extract, Giant extract, lion extract, perilla extract, linden extract, citrus extract, peony extract, ginger extract, ginger root extract, birch extract, Japanese horse chestnut extract, stevia extract, stevia fermented product, Atlantic kizuta extract, hawthorn extract, elderberry extract, and yarrow Extract, mint extract, sage extract, mallow extract, nematode extract, assembly extract, Sorakuhi extract, Daiou extract, soybean extract Vinegar, Thailand Saw extract, thyme extract, dandelion extract, tea extract, clove extract, Chinpiekisu, Tien-cha extract, pepper extract, Japanese angelica root extract, Toukinsenkaekisu, Tounin'ekisu,
Spruce extract, Dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract, buckwheat extract, lotus extract, parsley extract, birch extract, hamamelis extract, cypress extract, hinoki extract, loquat extract, dandelion extract, Fukinotou extract, Bukuryu extract, Butcher bloom extract, Grape extract, Grape seed extract, Loofah extract, Safflower extract, Peppermint extract, Bodhi extract, Button extract, Hop extract, Pine extract, Marronnier extract, Citrus extract, Mukuroji extract, Melissa extract, Mozuku Extract, peach extract, cornflower extract, eucalyptus extract, yukinoshita extract, yuzu extract, lily extract, yokuinin extract, mugwort , Extracts such as lavender extract, green tea extract, apple extract, rooibos tea extract, litchi extract, lettuce extract, lemon extract, forsythia extract, forsythia extract, rose extract, rosemary extract, roman chamomile extract, royal jelly extract, burberry extract It is mentioned as a thing.
Content of the plant extract in cosmetics is 0.01-30 mass% normally, 0.1-10 mass% is preferable and 1-5 mass% is more preferable.

Examples of the anti-inflammatory component include clarinone, grabridine, glycyrrhizic acid, glycyrrhetinic acid, and the like, and preferred are glycyrrhizic acid and its salt, glycyrrhetinic acid alkyl and its salt, and glycyrrhetic acid and its salt.
Content of the anti-inflammatory component in cosmetics is 0.01-30 mass% normally, 0.1-10 mass% is preferable and 1-5 mass% is more preferable.

Examples of the oil component include polar oil and volatile hydrocarbon oil.
Polar oils include synthetic ester oils such as isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, decyl oleate, hexyldecyl dimethyloctanoate, lactic acid Cetyl, myristyl lactate, lanolin acetate, isocetyl stearate, isocetyl isostearate, cholesteryl 12-hydroxystearylate, ethylene glycol di-2-ethylhexylate, dipentaerythritol fatty acid ester, N-alkylglycol monoisostearate, neopentyl dicaprate Glycol, diisostearyl malate, glycerin di-2-heptylundecanoate, trimethylolpropane tri-2-ethylhexylate May be mentioned trimethylolpropane triisostearate, tetra-2-ethylhexyl acid pentane erythritol, tri-2-ethylhexyl acid glycerin, trimethylolpropane triisostearate.

  Furthermore, cetyl 2-ethylhexanoate, 2-ethylhexyl palmitate, glyceryl trimyristate, glyceride tri-2-heptylundecanoate, castor oil fatty acid methyl ester, oleic acid oil, cetostearyl alcohol, acetoglyceride, palmitic acid 2 -Heptylundecyl, diisobutyl adipate, N-lauroyl-L-glutamic acid-2-octyldodecyl ester, di-2-heptylundecyl adipate, ethyl laurate, di-2-ethylhexyl sebacate, 2-hexyl myristate Decyl, 2-hexyldecyl palmitate, 2-hexyldecyl adipate, diisopropyl sebacate, 2-ethylhexyl succinate, ethyl acetate, butyl acetate, amyl acetate, triethyl citrate, octyl Toki Shishi runner formate may also be mentioned.

  Natural oils include avocado oil, camellia oil, turtle oil, macadamia nut oil, corn oil, mink oil, olive oil, rapeseed oil, egg yolk oil, sesame oil, persic oil, wheat germ oil, sasanca oil, castor oil, flaxseed oil, Safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, cinnagar oil, Japanese kiri oil, jojoba oil, germ oil, triglycerin, trioctanoic acid glycerin, triisopalmitic acid glycerin Etc.

  Examples of the volatile hydrocarbon oil include isododecane and isohexadecane.

As surfactants, fatty acid soap (sodium laurate, sodium palmitate, etc.), anionic surfactants such as potassium lauryl sulfate, alkylsulfuric triethanolamine ether, stearyltrimethylammonium chloride, benzalkonium chloride, laurylamine oxide Cationic surfactants such as
Betaine surfactants (alkyl betaines, amide betaines, sulfobetaines, etc.), imidazoline amphoteric surfactants (such as 2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy disodium salt), acylmethyl taurine Amphoteric surfactants such as
Sorbitan fatty acid esters (such as sorbitan monostearate and sorbitan sesquioleate), glycerin fatty acids (such as glyceryl monostearate), propylene glycol fatty acid esters (such as propylene glycol monostearate), hydrogenated castor oil derivatives, glycerin alkyl ether POE sorbitan fatty acid esters (POE sorbitan monooleate, polyoxyethylene sorbitan monostearate, etc.), POE sorbite fatty acid esters (POE-sorbitol monolaurate, etc.), POE glycerin fatty acid esters (POE-glycerin monoisosteare) Rate), POE fatty acid esters (polyethylene glycol monooleate, POE distearate, etc.), POE alkyl ethers (POE2-octyl) Decyl ether, etc.), POE alkylphenyl ethers (POE nonylphenyl ether, etc.), pluronic types, POE / POP alkyl ethers (POE / POP2-decyltetradecyl ether, etc.), tetronics, POE castor oil / cured castor Examples include oil derivatives (POE castor oil, POE hydrogenated castor oil, etc.), nonionic surfactants such as sucrose fatty acid esters, alkyl glucosides, and the like.

  Polyhydric alcohols include polyethylene glycol, glycerin, 1,3-butylene glycol, erythritol, sorbitol, xylitol, maltitol, propylene glycol, dipropylene glycol, diglycerin, isoprene glycol, 1,2-pentanediol, 2,4 -Hexylene glycol, 1,2-hexanediol, 1,2-octanediol and the like.

  Thickeners include guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, curdlan, methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, methylhydroxypropylcellulose, chondroitin sulfate, dermatan sulfate, glycogen, Heparan sulfate, hyaluronic acid, sodium hyaluronate, tragacanth gum, keratan sulfate, chondroitin, mucoitin sulfate, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, kerato sulfate, locust bean gum, succinoglucan, caronic acid, chitin, chitosan, carboxymethyl Chitin, agar, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, al Le-modified carboxyvinyl polymers, sodium polyacrylate, polyethylene glycol, and bentonite.

As powders, the surface may be treated, mica, talc, kaolin, synthetic mica, calcium carbonate, magnesium carbonate, silicic anhydride (silica), aluminum oxide, barium sulfate, etc. May be treated, bengara, yellow iron oxide, black iron oxide, cobalt oxide, ultramarine, bitumen, titanium oxide, zinc oxide inorganic pigments, surface may be treated, titanium mica, fish phosphorus foil, Pearl agent such as bismuth oxychloride, red 202, red 228, red 226, yellow 4, blue 404, yellow 5, red 505, red 230, red 223 which may be raked No., Orange No. 201, Red No. 213, Yellow No. 204, Yellow No. 203, Blue No. 1, Green No. 201, Purple No. 201, Red No. 204, organic dyes, polyethylene powder, polymeta Methyl acrylic acid, nylon powder, organic powders such as organopolysiloxane elastomers, and the like.

Examples of the UV absorber include paraaminobenzoic acid UV absorbers, anthranilic acid UV absorbers, salicylic acid UV absorbers, cinnamic acid UV absorbers, benzophenone UV absorbers, sugar UV absorbers, 2- (2 UV absorbers such as'-hydroxy-5'-t-octylphenyl) benzotriazole, 4-methoxy-4'-t-butyldibenzoylmethane, and the like.

  Moreover, the dosage form in the case of applying as an external preparation for skin can take any of the conventionally known lotion dosage forms, emulsion dosage forms, essence dosage forms, cream dosage forms, and powder-containing dosage forms.

<Example 1>
According to the formulation shown in Table 1 below, a cream that is an external preparation for skin of the present invention was prepared. The component (A) was heated to 80 ° C. and added to the component (B) which was also heated to 80 ° C. with stirring, and a uniform emulsion was prepared with a homogenizer. This was stirred and cooled, the component (C) was added at 45 ° C., and the cooling was stopped at 30 ° C. to obtain Cream 1.

<Example 2>
According to the formulation shown in Table 2 below, a cream that is an external preparation for skin of the present invention was prepared. First, in advance, the components (A) and (B) are heated to 70 ° C., the component (B) is added to the component (A) with stirring, and a crude liposome is prepared. Liposome dispersion (D). A part of the prepared liposome dispersion was added to (C) heated to 80 ° C. and mixed, and the mixture was added to the gelled (D) gradually and emulsified with stirring. Thus, a water-in-oil emulsifier type cream 2 (liposome-containing water-in-oil emulsifier type) cream 2 was obtained in the form of water droplets containing liposomes in the oil phase.

<Comparative Example 1>
Cream 3 was prepared by replacing the peonies extract of Example 1 with water.

<Test Example 4: Examination of wrinkle improvement effect of composition for preventing skin aging according to the present invention>
Using cream 1 and cream 2 prepared according to the methods described in Examples 1 and 2, and cream 3 prepared in Comparative Example 1, the wrinkle improving effect was examined according to the following method. That is, 12 panelists (women, age group 40-60) who are worried about wrinkles in the corners of the eyes are divided into 3 groups of 4 people, and cream 1, cream 2, and cream 3 are handed to each group. The wrinkle-improving effect was examined by comparing the eye corner replicas before and after the test twice a morning and evening for 8 weeks. The replica is a white one that does not transmit light, and the surface shape of the skin is transferred to this, and this replica is fixed to the sample stage of the stereomicroscope, irradiated with light at an angle of 45 degrees, and the replica is rotated, A shadow image (1 × 1 cm ² ) in the direction in which the shadow of the skin groove is strongly observed was taken into the image analysis apparatus. According to the wrinkles, the image has low brightness at deep wrinkles and high brightness at no wrinkles, forming a shadow. The luminance distribution in the shadow image is obtained, and with the median value of the luminance as a boundary, the bright spot with the luminance higher than the median value is converted to the maximum luminance, and the bright spot with the luminance less than the median value is converted to the luminance 0, and binarization is performed. The area ratio of the shaded portion (the portion with 0 luminance) was obtained. The wrinkle improvement rate (%) was determined by (area ratio of shadow before test−area ratio of shadow after test) / (area ratio of shadow before test) × 100. The results are shown in Table 1 as the mean value ± standard deviation of 4 people in each group. From the results in Table 3, it can be seen that Cream 1 and Cream 2 of the present invention have an excellent wrinkle improving action.

The composition for preventing skin aging of the present invention can be applied to anti-aging cosmetics, anti-aging foods and the like.
It is possible to provide a composition having an effect of preventing skin aging that could not be achieved by a conventional composition.

Claims (7)

  A composition for preventing skin aging, comprising an ultraviolet damage inhibitor and a near infrared damage inhibitor.   The composition for preventing skin aging according to claim 1, wherein the near-infrared damage inhibitor is a sustained release carrier containing an MMP-1 inhibitor.   The skin aging prevention according to claim 1, wherein the near-infrared damage inhibitor comprises one or more selected from the group consisting of peony extract, sage extract, chamomile extract, geno shoko extract, yukinoshita extract, pomegranate extract and altea extract. Composition.   The UV damage inhibitor is loquat extract, carrot extract, ginseng extract, neem extract, assembly extract, rosemary extract, clove extract, cedar extract, clove extract, loofah extract, mugwort extract, carambola extract, assembly extract, forsythia extract, The skin aging according to any one of claims 1 to 3, comprising at least one selected from the group consisting of an auren extract, an arnica extract, a melissa extract, a clara extract, a yarrow extract, a ginger extract, and a hypericum extract. Prevention composition.   The skin external preparation containing the composition for skin aging prevention of any one of Claims 1-4.   The skin external preparation according to claim 5, which is a cosmetic.   The foodstuff containing the composition for skin aging prevention of any one of Claims 1-4.