JP2008105984A - Collagen production promoter, skin care preparation for external use, bathing agent, and food and drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a collagen production promoter that causes a skin fibroblast to produce collagen which exerts large influences on elasticity and tightness of the skin, and to provide a skin care preparation for external use, a bathing agent, and foods and drinks that comprise the collagen production promoter and exhibit effects of improving tightness of the skin and wrinkles. <P>SOLUTION: The collagen production promoter comprises one or two or more selected from among Sophora flavescens extracts, Polygala tenuifolia extracts, Diospyros kaki extracts, Salvia miltiorrhiza extracts, Peonia lactiflora extracts, puer extracts, arbutin, and oligosaccharide alginates. The skin care preparation for external use, the bathing agent, and foods and drinks comprise the collagen production promoter and exhibit effects of improving tightness of the skin and wrinkles. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a collagen production promoter that plays an important role in skin elasticity, tension and moisture. Furthermore, it is related with the skin external preparation, the bath preparation, and food and drink containing a collagen production promoter.

  In recent years, beauty awareness has increased with the aging of society and the advancement of women in society. However, the function of the skin decreases with age, and aging causes loss of elasticity and tension and wrinkles. This is due to a decrease in the amount of collagen and hyaluronic acid present in the skin.

The skin consists of three layers: the subcutaneous tissue, the dermis, and the epidermis. The dermis has the most influence on the skin's functions, such as the elasticity of the skin. This dermis is rich in collagen and hyaluronic acid. Yes. These collagens and hyaluronic acid are known to be produced by dermal fibroblasts present in the dermis and play an important role in maintaining the elasticity of the skin.
Collagen accounts for approximately 30% of human protein, of which approximately 70% is present in the dermis. Collagen works to hold cells together, but it decreases with age, and it is thought that wrinkles and sagging occur in the skin.

  Furthermore, conventional collagen plays an important role in giving mechanical strength to various connective tissues, and currently affects various activities such as cell metabolism, morphology, proliferation, differentiation, and migration. It is known that it is expected to be applied to the treatment of collagen diseases, the prevention of keloids, and the prevention of skin aging by regulating the amount of collagen production in cells such as fibroblasts.

  As cosmetics for improving skin wrinkles and improving skin elasticity, Patent Document 1 discloses cosmetics containing retinol as an active ingredient. Further, Patent Document 2 discloses a nanocapsule containing a lipophilic active agent as a composition used for the care of keratin substances (skin etc.).

However, the retinol and lipophilic active agent described in Patent Document 1 or 2 have strong skin irritation and are unstable substances, and therefore require special formulation techniques. Furthermore, Patent Documents 1 and 2 disclose nothing about the fact that specific plant extracts such as Clara extract or specific compounds such as arbutin promote collagen production and prevent skin aging.
JP 2003-81737 A JP 2003-292420 A

  The present invention relates to a collagen production promoter that greatly affects the elasticity and tension of the skin and produces collagen that is important in maintaining youthful skin to the skin fibroblasts, and the skin tension containing the collagen production promoter. And an object of the present invention is to provide a composition having an effect of improving wrinkles.

  As a result of intensive studies to solve the above problems, the present inventors have found that specific plant extracts, arbutin and alginic acid oligosaccharides have an action of promoting collagen production. Was completed.

That is, the present invention comprises a collagen production promoter characterized by containing one or more selected from a clara extract, a citrus extract, a oyster extract, a tanjin extract, a peony extract, a puer extract, an arbutin, and an alginate oligosaccharide. I will provide a.
Furthermore, this invention provides the skin external preparation, the bath preparation, or food-drinks characterized by including the said collagen production promoter.

  Collagen production promoter containing, as an active ingredient, one or more selected from the clara extract, citrus extract, oyster extract, tanjin extract, peony extract, puer extract, arbutin and alginic acid oligosaccharide of the present invention is a skin fiber Has an effect of promoting collagen production in blast cells. These collagen production promoters can increase the amount of fibroblast collagen in the skin, and as a result, can improve skin wrinkles and sagging, anti-aging cosmetics, anti-aging bath agents and anti-aging bath agents. Use as an active ingredient of aging foods and drinks can be expected.

  The collagen production promoter of the present invention is characterized by containing one or more selected from Clara extract, Cyprinus extract, oyster extract, Tanjin extract, peony extract, Puaru extract, arbutin and alginic acid oligosaccharide. It is.

As the collagen production promoter of the present invention containing two or more selected from the plant extract, arbutin and alginic acid oligosaccharides,
A combination of Clara extract and alginate oligosaccharide, arbutin, Itohime extract, oyster extract, peonies extract, tanjin extract, or puer extract;
A combination of Itohime extract and arbutin, peony extract, oyster extract, tanjin extract, puer extract, or alginate oligosaccharides;
A combination of Clara extract, Cypress extract, and alginate oligosaccharides;
A combination of Itohimehagi extract, arbutin, and peony extract;
A combination of Itohime extract, arbutin, and oyster extract;
Those containing a combination selected from the above are preferred because they are particularly excellent in the action of improving wrinkles and sagging of the skin.

  The Clara extract used in the present invention is extracted by using the roots of the leguminous plant Clara, but can be extracted by using the above-mentioned part and a plant belonging to the same genus.

  The extract of Itohimehae used in the present invention is extracted using the root of Itohimehagi, which is a plant belonging to the human family, but can also be extracted using the above-mentioned genus plant.

  The oyster extract used in the present invention is extracted using oyster leaves of a cynoaceae plant, but can be extracted using the above-mentioned part and a genus plant.

  The tanjin extract used in the present invention is extracted using roots of tannins of Labiatae plants, but can be extracted using the above-ground parts and the same genus plants.

  The peony extract used in the present invention is extracted using a peony root that has been removed from the peony root, but can be extracted from the above-ground part and a plant belonging to the same genus.

  The puer extract used in the present invention is extracted by using a so-called puer leaf obtained by post-fermenting tea leaves of a camellia plant with black koji mold or the like.

  In the present invention, as a plant extract such as Clara extract, citrus extract, oyster extract, tanjin extract, peony extract, puer extract, etc. It can be used after being crushed or ground. Furthermore, the extract obtained by extracting these with a solvent, and the non-volatile content obtained by evaporating or lyophilizing the extraction solvent from the extract can be used.

  Examples of the extraction solvent used herein include water, lower alcohols such as methanol, ethanol, ethylene glycol, propylene glycol, and 1,3-butylene glycol, ketones such as acetone, esters such as ethyl acetate, and diethyl ether. Various solvents, such as ethers and aromatics, such as toluene, are mentioned, It can use individually or in combination of 2 or more types.

  Arbutin used in the present invention is a natural type (β-glucoside type) glycoside contained in plants such as cowberry, walrus, and pear, and has a chemical name of 4-hydroxyphenyl-β-D-glucosipyrano. Sid's can be used.

  As the alginate oligosaccharide used in the present invention, it is preferable to use a polymer alginic acid or a polymer alginate (sodium salt, magnesium salt, potassium salt, etc.) whose molecular weight is adjusted to 2000-20000. The decomposition method may be acid or alkali decomposition, enzymatic decomposition, thermal decomposition, etc., regardless of the method, and after converting high molecular alginic acid to low molecular alginic acid, low molecular alginate is used. But it ’s okay.

  The plant extract used in the present invention is generally used as a component of medicines or folk medicines, foods and cosmetics, and its safety has been confirmed.

  The collagen production promoter of the present invention contains one or more selected from the above plant extracts, arbutin and alginic acid oligosaccharides, and the total amount added to the collagen production promoter varies depending on the dosage form. The evaporation residue may be used as it is, and the blending amount may be appropriately adjusted depending on the intended use. Also, the amount of the collagen production promoter of the present invention is not particularly limited, and is appropriately determined depending on the use and application. Can be adjusted.

  The collagen production promoter of the present invention can be applied to various forms such as treatment for external or internal materials. In addition, it can be used in combination with a drug used for treatment of ordinary external or internal materials, and the effect of the present invention is more easily exhibited by the combined drug.

  Collagen production promoters of the present invention include pharmaceuticals, quasi-drugs, topical or systemic skin cosmetics, various skin external preparations such as medicinal or cosmetic preparations applied to the scalp / hair, bath preparations and It can mix | blend with food and drink.

  The skin external preparation, bath preparation, and food and drink of the present invention contain one or more of the collagen production promoters. The total amount of the collagen production promoter to the skin external preparation or the like varies depending on the dosage form, but generally, the collagen production promotion is based on the mass of the skin external preparation, bath preparation, and overall food and drink. In terms of the amount of plant extract, arbutin and alginic acid oligosaccharide contained in the agent, 0.01% by mass to 10% by mass, preferably 0.025% by mass to 5% by mass, and more specifically 0.05% by mass. It is desirable to contain so that it may become% -2.5 mass%.

  The skin external preparation and bath preparation of the present invention include, in addition to the collagen production promoter, known functional components conventionally used in normal skin external preparations or bath preparations, such as moisturizers, emollients, blood circulations. Accelerators, cell activators, antioxidants, anti-inflammatory agents, antibacterial agents, whitening agents, peroxide inhibitors and the like can be blended.

More specifically, known functional ingredients include humectants such as glycerin, butylene glycol, urea, and amino acids; emollients such as squalane, macadamia nut oil, olive oil, jojoba oil, and silicone oil; vitamin Es, pepper tincture, and the like. Blood circulation promoters; cell activators such as nucleic acids, antioxidants such as dibutylhydroxytoluene (BHT), dibutylhydroxyanisole (BHA), tocopherol acetate; anti-inflammatory agents such as glycyrrhizin and allantoin; hinokitiol, benzalkonium chloride, Various functional components such as antibacterial agents such as chlorhexidine salt and parahydroxybenzoate; whitening agents such as ascorbic acid; peroxide inhibitors such as superoxide dismutase (SOD) can be blended.
In addition, various extracts derived from plants, animals, and microorganisms such as hornon extract, ginkgo biloba extract, placenta extract, and lactic acid bacteria culture extract can be added and used without limitation.

  The external preparation for skin of the present invention is an agent that can be applied externally, and there is no particular limitation on the dosage form, for example, paste, cream, gel, ointment, lotion, emulsion, pack, powder, haptic agent, etc. It can be illustrated.

  There is no restriction | limiting in particular in the dosage form of the bath preparation of this invention, For example, liquid preparations, such as solid preparations, such as a powder and a granular form, an emulsion, and a paste form, etc. can be illustrated.

  In addition to the collagen production promoter, various materials that are usually used in foods can be used in combination with the food and drink of the present invention without any particular limitation.

  The dosage form of the food and drink of the present invention includes all applicable forms, for example, solid preparations such as biscuits, cookies, tablets, capsules, candy, gum, and powder, liquid preparations such as beverages, and semisolids such as jelly Examples include preparations.

  In addition, the external preparation for skin, bath preparation and food and drink of the present invention include base ingredients such as surfactants and fats and oils, and thickeners, preservatives, isotonic agents as necessary. Various additives such as an agent, an antioxidant, an ultraviolet absorber, a chelating agent, a fragrance, and a coloring agent can be used in combination.

  Although it does not specifically limit as said surfactant, A general nonionic surfactant, anionic surfactant, a cationic surfactant, and an amphoteric surfactant can be used. For example, alkylene oxide adduct of higher alcohol, alkylene oxide adduct of higher alkylamine, alkylene oxide adduct of higher fatty acid, alkylene oxide adduct of higher fatty acid amide, fatty acid ester of polyhydric alcohol, alkylene oxide adduct of hardened castor oil , Nonionic surfactants such as alkylene oxide adducts such as polyethylene glycol sorbitan alkyl esters and sterols; anions such as sodium alkyl sulfate, sodium alkylylmethyl taurate, sodium α-olefin sulfonate, sodium polyoxyalkylene alkyl ether sulfate Surfactants; Cationic surfactants such as alkylpyridinium chloride and distearyl dimerylammonium chloride; Sodium aminopropionate Amphoteric surfactants such as alkylpolyaminoethylglycine the like. And these surfactant can select and use 1 type (s) or 2 or more types.

  The base component usable in the present invention is not particularly limited, and examples thereof include olive oil, camellia oil, avocado oil, macadamia nut oil, apricot oil, jojoba oil, squalane, squalene, horse oil, paraffin, silicon and the like. And generally known fats and oils.

  Although it does not specifically limit as a thickener which can be used in this invention, For example, polyvinyl alcohol, polyvinyl acrylamide, polyethyleneglycol, and these various derivatives; Celluloses, such as a hydroxyalkyl cellulose, and its derivative; Dextran, Examples include gums such as gelatin, gum arabic, and gum tragacanth; and water-soluble polymers such as carboxyvinyl polymer.

  Examples of the preservative that can be used in the present invention include, but are not limited to, parahydroxybenzoic acid esters, paraoxybenzoic acid salts and derivatives thereof, phenoxyethanol, hinokitiol, benzalkonium chloride, chlorhexidine salts, and the like. .

  The isotonic agent that can be used in the present invention is not particularly limited, and examples thereof include inorganic salts such as sodium chloride, potassium chloride, and calcium chloride.

  Although it does not specifically limit as an ultraviolet absorber which can be used in this invention, For example, a para amino benzoic acid, a benzophenone series ultraviolet absorber, a benzotriazole type ultraviolet absorber, etc. are mentioned.

  Although it does not specifically limit as a chelating agent which can be used in this invention, For example, ethylenediaminetetraacetic acid, phytic acid, a citric acid, these water-soluble salts, etc. are mentioned.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples.
(1) Production method of plant extract The plant extract is obtained by extracting each part of each plant shown in Table 1 below under reflux of a 50 mass% ethanol aqueous solution over 1 hour, followed by filtration, and from the obtained extract. Water and ethanol were distilled off under reduced pressure to obtain each plant extract. The obtained plant extract was used for the Example shown below.

(2) Production method of alginic acid oligosaccharide 1.5 g of commercially available sodium alginate SKAT-ULV (manufactured by Kimika), 0.05 g of ammonium phosphate, 0.01 g of yeast extract, and 2 g of sodium chloride in 100 g of ion-exchanged water Dissolved and sterilized by autoclave at 2.1 atm, 121 ° C. for 20 minutes. The alginate solution after sterilization was platinized with Pseudoalteromonas halloplastic (ITM 222), which is an alginate-degrading bacterium, and pre-cultured at room temperature for 8 hours.

  Next, 1 mL of the obtained preculture solution was ion-exchanged with sodium alginate decomposition medium (0.15 g of ammonium phosphate, 0.01 g of yeast extract, 9.0 g of sodium alginate (manufactured by Kimika), 6.0 g of sodium chloride) It was dissolved in 300 g of water and added to an autoclave that had been sterilized at 2.1 atm, 121 ° C. for 20 minutes, and then cultured with shaking at room temperature for 48 hours.

  Centrifugation operation (10000 rpm × 10 minutes) was performed to remove bacterial cells from the sodium alginate decomposition solution after shaking culture, and a supernatant was obtained. Add the same amount of methanol to the resulting supernatant, precipitate high molecular weight (tens of thousands) sodium alginate from the decomposed sodium alginate solution, and filter and contain low molecular weight sodium alginate oligosaccharides. A filtrate was obtained.

  To remove impurities such as sodium chloride from the filtrate containing sodium alginate oligosaccharide and perform purification, gel filtration operation (Sephadex LH-20, Pharmacia Fine Chemicals) was performed. After the gel filtration operation, the obtained liquid was concentrated and dried with a freeze dryer to obtain 5.4 g (yield 60%) of the desired sodium alginate oligosaccharide (average molecular weight: about 2000).

In addition, alginate oligosaccharide sodium having a weight average molecular weight of about 20,000 was obtained in the same manner as above except that the shaking culture time was changed from 48 hours to 8 hours.
Further, the obtained sodium alginate oligosaccharide having a weight average molecular weight of about 2000 is operated together with an ion exchange resin (Amberlite 1006F H, manufactured by Rohm and Haas Japan) to obtain an alginate oligosaccharide having a weight average molecular weight of about 2000. Got.

  The weight average molecular weight was measured using an aqueous gel permeation chromatography apparatus GPC-8020 (manufactured by Tosoh Corporation). The measurement conditions were as follows: G3000 (manufactured by Tosoh Corp.) and G5000 (manufactured by Tosoh Corp.) were directly connected to the column, column temperature 40 ° C., column flow rate 1.0 mL / min, eluent 0.1M NaCl aqueous solution, RI detection, and the weight average molecular weight is a value obtained by converting PEG (polyethylene glycol) as a standard.

  The obtained sodium alginate oligosaccharide and alginate oligosaccharide were used in the following examples.

(3) Arbutin Arbutin manufactured by Wako Pure Chemical Industries, Ltd. was used in the following examples.
(I) Collagen production promoter [Example 1]
Arbutin was prepared to 10 mg / mL, and then sterilized using a 0.2 μm syringe filter in a clean bench. Then, sterilized purified water was added to adjust to 2 mg / mL to prepare a diluted solution of arbutin to be added to the medium.
Next, normal human dermal fibroblasts manufactured by Kurashiki Boseki Co., Ltd. were differentiated and cultured according to a prescribed preparation method. The medium used was a low serum medium (Medium 106S) for normal human skin fibroblast proliferation supplemented with a low serum growth additive (LSGS).
The medium was seeded with a solution of normal human adult fibroblasts (500000 cells / mL), placed in a humidified incubator at 37 ° C., 5% -CO ₂ , the medium was changed every other day, and cultured for 6 days.
Six days later, cells were peeled from the petri dish to prepare a cell suspension (15000 cell / mL). 1 mL of the cell suspension was seeded on a 24-well plate and cultured for 2 days. At the time of medium exchange, 10 μL of the diluted arbutin solution (final concentration becomes 20 μg / mL) was added, and further cultured for 2 days. The medium was changed every two days, and the medium was collected on the 8th day of cell seeding. The recovered medium is present in the supernatant after 48 hours of culture from the 6th day to the 8th day according to the usage method using Procollagen type I C-peptide (PIP) EIA Kit of TaKaRa. The amount of type I procollagen C-terminal propeptide (hereinafter abbreviated as PIP), which is a collagen precursor protein, was measured. This PIP reflects the amount of collagen.
The amount of PIP at this time was the same as that of Comparative Example 1, that is, the amount of PIP of Comparative Example 1 in which sterilized purified water was added instead of the diluted solution of collagen production promoting component (arbutin) and the other operations were the same. Compared with, collagen production promoting ability was 175%.

[Examples 2 to 37]
The same procedure as in Example 1 was conducted except that the collagen production promoting component and the final concentration added were changed as shown in Table 2, and the amount of PIP obtained and the amount of PIP in Comparative Example 1 were similarly determined. The comparison results are shown in Table 2.

  As shown in the results of Examples 1 to 37 in Table 2, those using various plant extracts, arbutin, alginic acid oligosaccharides, and compositions thereof as collagen production promoting components were effective against collagen production in normal human skin fibroblasts. It can be seen that the collagen production promotion effect is significantly exhibited.

(II) Formulation examples of external preparations for skin Preparations of external preparations for skin (skin lotion, emulsion, cream, lipstick, body cream) formulated with collagen production promoters with the compositions shown in Tables 3 to 17 were prepared. The preparation method is as follows. The unit of the blending amount is g, and the “remaining amount” of purified water in the table is an amount that makes the total amount 100 g. Further, the “remaining amount” of castor oil in the table of the lipstick is also an amount that makes the total amount 100 g.

Lotion (Examples 38-53, Comparative Example 2)
Each of A component and B component of Tables 3-5 was heated and melt | dissolved at 80 degreeC, and B component was gradually added and emulsified to A component, stirring. Then, it cooled, stirring, and after adding C component at 40 degreeC, preparation was complete | finished at 35 degreeC.

Latex (Examples 54-69, Comparative Example 3)
Each of D component and E component of Tables 6-8 was heated and melt | dissolved at 80 degreeC, it added with stirring D component to E component, and it emulsified with the homomixer after making it uniform. Then, it cooled, stirring with a handle mixer, added F component at 40 degrees C or less, and complete | finished preparation.

Cream (Examples 70 to 85, Comparative Example 4)
The G component and the H component in Table 9 were each dissolved by heating to 75 ° C., and the H component was added little by little while stirring the G component with a paddle mixer. Then, it cooled, stirring, and added I component at 40 degreeC, Then, preparation was complete | finished at about 35 degreeC.

Lipstick (Examples 86 to 101, Comparative Example 5)
The J components in Tables 12 to 14 were dissolved by heating to 85 ° C., and the J component was added little by little while stirring the K component and mixed uniformly. Then, it cooled, stirring, and after adding L component at 60 degreeC vicinity, it cooled further. Thereafter, the container was filled and the preparation was completed.

Body cream (Examples 102 to 117, Comparative Example 6)
M component and N component of Tables 15-17 were each heated to 75 degreeC, and N component was added little by little, stirring M component with a paddle mixer. Then, it cooled, stirring, O component was added at 45 degrees C or less, and preparation was complete | finished.

Evaluation Regarding the external preparations for skin of Examples 38 to 117 and Comparative Examples 2 to 6, a monitor test was conducted on women in their 30s and 40s who had problems of wrinkles and tension of the skin and a decrease in elasticity. Went.
For each topical skin preparation, 3 women (17 groups x 5 prescriptions) for a total of 255 people, lotions, emulsions and creams applied to the face twice a day (morning and evening), lipstick Applying to the lips three times a day (morning, noon, evening) for the skin, and one month for a monitor test for normal life except for applying the body cream to the body twice a day (morning, evening) Continued for a while. Table 18 shows the results of evaluating the feeling of use according to the following criteria after one month. None of the patients complained of skin abnormalities during the period of use.
Effective: Feels the skin tension and elasticity compared to before use Slightly effective: Feels the skin tension and elasticity slightly compared to before use No effect: No change from before use

  In the external preparations for skin of Examples 38 to 117 in which the collagen production promoter of the present invention was blended, there were many people who felt tension or elasticity at each site, but the collagen production promoter of the present invention was not blended. Since it was evaluated that there was no change in the external preparations for skin of Comparative Examples 2 to 6, the external preparation for skin containing the collagen production promoter of the present invention was used for improving the tension or elasticity of the skin or lips. It was significant.

(III) Formulation of Bath Agent Next, a formulation of an anti-aging bath agent (bath agent) having a composition shown in Tables 19 to 21 and a collagen production promoter was prepared. The preparation method is as follows. The unit of the blending amount is g, and the “remaining amount” of purified water in the table is an amount that makes the total amount 100 g.

Bath agent (Examples 118 to 133, Comparative Example 7)
After mixing P component and Q component of Tables 19-21 until it became uniform respectively, P component and Q component were mixed, and it mixed well until it became uniform, and preparation was complete | finished.

Evaluation Regarding the bath preparations of Examples 118 to 133 and Comparative Example 7, a monitor test was conducted on women in their 30s and 40s who have problems of wrinkles and tension of the skin and a decrease in elasticity. .
For each bathing agent, a total of 51 women (17 groups) for a total of 51 women will be able to perform a normal test, except that 30 mL of bathing agent is dissolved in 180 liters of hot water at the time of daily bathing. Continued for a month. Table 22 shows the results of evaluating the feeling of use according to the following criteria after one month. None of the patients complained of skin abnormalities during the period of use.
Effective: Feels the skin tension and elasticity compared to before use Slightly effective: Feels the skin tension and elasticity slightly compared to before use No effect: No change from before use

  In the bath preparations of Examples 118 to 133 in which the collagen production promoter of the present invention was blended, there were many people who felt tension and elasticity on the skin, but comparative examples in which the collagen production promoter of the present invention was not blended. Since it was evaluated that there was no change in the bathing agent of No. 7, the bathing agent containing the collagen production accelerator of the present invention was significant for improving the skin tension and elasticity.

(IV) Food / Beverage Formulation Next, a formulation of anti-aging food / beverage (bread, jelly, sausage, supplement (tablet)) blended with a collagen production promoter with the composition shown in Tables 23 to 30 was prepared. The preparation method is as follows. The unit of the blending amount is g, and the “remaining amount” of purified water in the table is an amount that makes the total amount 100 g.

Bread (Examples 134 to 141, Comparative Example 8)
A mixture of all the R components in Tables 23 and 24 was added to the mixture of the S and T components heated to 40 ° C., and kneaded well when the mixture became uniform. Primary fermentation was performed at 35 ° C. for 40 minutes, and kneaded again so as to remove air. After secondary fermentation at 40 ° C. for 40 minutes, baking was performed at 180 ° C. for 25 minutes.

Jelly (Examples 142-149, Comparative Example 9)
The U component was dissolved in 80 ° C. hot water in which the V component was dissolved in the W component in Tables 25 and 26 and mixed well. After pouring 10 g into the mold, it was hardened in a refrigerator to complete the preparation.

Sausage (Examples 150 to 157, Comparative Example 10)
The X component and Y component in Tables 27 and 28 were mixed well, cooled in a refrigerator, and then packed in the intestines of salted sheep. The intestinal stuffed product was smoked at 60 to 70 ° C. for 1 hour and further dried for 1 hour. The dried product was boiled with hot water of about 70 ° C. for about 30 minutes and sterilized. And it dried again, and preparation was complete | finished.

Supplement (Tablet) (Examples 158 to 165, Comparative Example 11)
A mixture of the AA component and the AB component in Tables 29 and 30 was mixed into tablets using a tableting machine to form tablets (0.3 g).

Evaluation Regarding the food and drink of Examples 134 to 165 and Comparative Examples 7 to 11, a monitor test was conducted for women in their 30s and 40s who have worries about skin wrinkles and tension and a decrease in elasticity. went.
For each food and drink, 3 women (9 groups x 4 prescriptions) for a total of 108 people, for bread, eat 90g of the above example or comparative example bread as a staple food at the time of daily breakfast, about jelly Ingesting 50 g of the jelly of the above example or comparative example after dinner every day, about sausage, eating about 60 g of the sausage of the example or comparative example as a side meal at dinner every day, about supplements (tablets) The monitoring test for normal life was continued for one month except that 5 supplements (1.5 g) of the supplements (tablets) of the examples or comparative examples were taken 3 times daily. Table 31 shows the results of evaluating the feeling of use according to the following criteria after one month. None of the patients complained of skin abnormalities during the period of use.
Effective: Feels the skin tension and elasticity compared to before use Slightly effective: Feels the skin tension and elasticity slightly compared to before use No effect: No change from before use

  In the foods and drinks of Examples 134 to 165 blended with the collagen production promoter of the present invention, there were many people who felt tension and elasticity on the skin, but comparative examples not blended with the collagen production promoter of the present invention. Since it was evaluation that there was no change in the foods and drinks of 8-11, all the foods and drinks which mix | blended the collagen production promoter of this invention were significant with respect to the improvement of skin tension and elasticity.

  Clara extract, citrus extract, oyster extract, tanjin extract, peony extract, puer extract, arbutin or alginic acid oligosaccharide and a mixture thereof have an action of promoting collagen production and are useful as a collagen production promoter. The collagen production promoter of the present invention increases the amount of collagen produced in fibroblasts. As a result, wrinkles and sagging can be prevented, alleviated, and improved, with the aim of preventing and improving the aging phenomenon. It can be used for various skin external preparations such as cosmetics, bath preparations and foods and drinks.

Claims (4)

  A collagen production promoter characterized by containing one or more selected from Clara extract, Cypress extract, Oyster extract, Tanjin extract, Peonies extract, Pual extract, Arbutin and Alginate oligosaccharide.   An external preparation for skin, comprising the collagen production promoter according to claim 1.   A bath preparation comprising the collagen production promoter according to claim 1.   A food or drink comprising the collagen production promoter according to claim 1.