JP3171637B2 - Anti-aging cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic having an excellent antiaging effect, which can be suitably used for basic cosmetics, makeup cosmetics, hair cosmetics, bath preparations and the like.

[0002]

2. Description of the Related Art The need for anti-aging cosmetics has been considered so far, but the mechanism and definition of aging have not been clarified. They have been measuring the moisturizing state and the elasticity of the skin as well as visually observing and judging the color of the skin.

[0003] Recent studies on aging show that the main causes of skin aging are collagen cross-linking reaction, reduction of mucopolysaccharides such as hyaluronic acid, and cell damage by ultraviolet rays. Becoming known, it is enough to keep moisture by simply mixing biochemical products such as mucopolysaccharide and collagen and synthetic high polymer products like conventional cosmetics, and to prevent skin aging. I couldn't do it.

[0004] Therefore the present inventors have conducted extensive research in view of the point of the decomposition products which are E was treated with several proteases After extracting rice or rice bran with an alkaline solution, lipid peroxidation Since it has a production inhibitory action, a superoxide dismutase (SOD) -like activity, a collagen synthesis accelerating action, and a power of restoring cell damage caused by ultraviolet rays, cosmetics containing the degraded product are useful for producing fibroblasts in the skin. By acting on cells, it synthesizes collagen, one of the important components of the dermis, and by preventing lipid peroxides, it prevents skin troubles. It has been found that the present invention has an excellent cosmetic effect of restoring cell damage and maintaining a youthful skin condition, and has completed the present invention.

[0005]

That is, the present invention provides an alkaline extract of rice and / or rice bran comprising two or more protein components.
The present invention relates to an anti-aging cosmetic, which comprises a degraded product obtained by treatment with a decomposing enzyme.

[0006]

Action and Examples The anti-aging cosmetic composition of the present invention contains a decomposed product obtained from rice and / or rice bran.
The rice and rice bran are first subjected to an alkali extraction treatment. If rice is used here, grind it in advance.

Solvents used in the alkali extraction treatment include, for example, purified water; lower alcohols such as ethanol; ethylene glycol, propylene glycol,
Polyols such as glycerin and 1,3-butylene glycol; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; ketones such as acetone; esters such as ethyl acetate; hydrocarbons such as hexane, chloroform and benzene And the like. These may be used alone or as a mixture of two or more. Among these, purified water or a solvent in which purified water or a mixture of one or more of ethanol, glycerin, and 1,3-butylene glycol is preferable from the viewpoint of wide application to cosmetics.

When these mixed solvents are used, the volume ratios of purified water and ethanol are 1 to 25: 1, purified water and glycerin are 1 to 15: 1, and purified water and 1,3-butylene glycol are 1 to 15: 1. : 1 is preferred.

Examples of the alkali preparation agent used in the alkali extraction treatment include sodium salts such as sodium hydroxide and sodium carbonate and potassium salts such as potassium hydroxide. The pH of these is set to 7.5 to 14.0. You. Among them, sodium hydroxide and sodium carbonate are preferable because the target pH can be set at a low concentration.

The alkali extraction treatment time varies depending on the type of the solvent and the alkali preparation agent used, the set pH, the extraction temperature and the like, but is usually 8.5 to 14.0 at the set pH and 6 hours to 7 at room temperature.
Days are preferred. The extraction temperature is preferably 4 to 40.
° C.

The alkaline extract obtained as described above is then subjected to an enzyme treatment.

Examples of the proteolytic enzymes used in the above-mentioned enzyme treatment include actinases, pepsins such as pepsin, trypsins such as trypsin, papains such as papain and chymopapain, glycylglycine peptidase, carboxypeptidase, amino acid and the like. Examples include peptidases such as peptidase and bromelain, and two or more of these are used. Of these, actinase , pepsin, trypsin, papain, glycylglycine peptidase, carboxypeptidase,
It is preferable to use a combination of one or more proteases such as aminopeptidase, bromelain, chymopapain and chymotrypsin, and particularly preferable is a combination of actinase, pepsin and trypsin.

[0013] The amount of proteolytic enzyme, 0.0005 parts to the alkaline extraction solution 100 parts per one treatment (parts by weight, hereinafter the same), preferably from 0.001 to 0. 005 parts, in total 0.003-0.
It is preferable that the amount is about 015 parts from the viewpoint of the effect.

[0014] Enzyme treatment time varies depending on the type and the decomposition temperature of the proteolytic enzyme to be used, it is generally 1 to 24 hours is desirable. The decomposition temperature is about 30 to 50 ° C. The decomposed product thus obtained may be concentrated under reduced pressure to adjust the concentration, or the decomposed product may be used as a powder by a freeze-drying method or a spray-drying method.

[0015] The pH of the obtained decomposed product solution is preferably adjusted to pH 4 to 8 from the viewpoint of safety on the skin.

As described above, the decomposed product of the present invention has an excellent inhibitory action on the production of lipid peroxide, an SOD-like activity, a collagen synthesis accelerating action and an ultraviolet ray damage relieving action on human skin, and has an effect on skin aging. It can prevent and maintain youthful and healthy skin condition.

The anti-aging cosmetic of the present invention contains the above-mentioned decomposed product, and the amount thereof varies depending on the type of the cosmetic, and thus cannot be determined unconditionally. The amount is 0.0005 to 5.0 parts, preferably 0.005 to 1.0 part in terms of the solid content of the decomposition product per 100 parts of the raw material. When the amount is less than the above range, the effect of blending the decomposed product tends to decrease, and when the amount exceeds the range, it is technically necessary to stably blend the composition into cosmetics. Tends to be difficult.

Further, the anti-aging cosmetic composition of the present invention may contain, in addition to the decomposition products, excipients and fragrances generally used in cosmetics, oils and fats, surfactants, humectants, pH adjusters. Various cosmetic ingredients such as agents, thickeners, preservatives, antioxidants, ultraviolet absorbers, pigments, detergents, desiccants, and emulsifiers are appropriately blended.

Examples of the fats and oils include vegetable oils generally used in cosmetics such as liquid paraffin, paraffin, cetanol, avocado oil, olive oil, jojoba oil, coconut oil, etc .; beef tallow, lard, horse tallow, turtle oil , Mink oil,
Animal fats and oils such as parserin oil and squalane; and synthetic fats and oils such as methyl polysiloxane, behenyl alcohol, glyceryl tricaprylate, glyceryl trioctanoate, glyceryl triisopalmitate, and silicone oil.

Examples of the surfactant include anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate and diethanolamide laurate; and surfactants such as stearyltrimethylammonium chloride, cetyltrimethylammonium chloride and benzalkonium chloride. Cationic surfactants: Nonionic surfactants such as glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene hydrogenated castor oil, sucrose ester, fatty acid amide, etc. Is raised.

Examples of the humectant include glycerin, propylene glycol, 1,3-butylene glycol,
Synthetic moisturizers such as sodium pyrrolidonecarboxylate and pantothetein-S-sulfonate; natural moisturizers such as hyaluronic acid, collagen, elastin, placenta extract, royal jelly, and microbial fermentation solution.

Examples of the pH adjuster include organic acids such as sodium citrate and citric acid and salts thereof.

Examples of the thickener include carboxymethyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer,
Examples include polyvinyl alcohol, tragacanth gum, sodium alginate, carrageenan and the like.

Examples of the preservative include paraoxybenzoic acid esters such as methylparaben, ethylparaben, propylparaben and butylparaben, phenoxyethanol, ethanol, dehydroacetic acid and the like.

Examples of the antioxidant include vitamin E, butyloxytoluene (BHT), butyloxyanisole (BHA) and the like.

Examples of the pigment include red iron oxide, yellow iron oxide, black iron oxide, titanium oxide, nylon powder, sericite, mica, and talc.

Examples of the detergent include sodium lauryl sulfate.

Examples of the excipient include sodium sulfate.

Examples of the emulsifier include soybean lecithin oil and the like.

The amount of each of these cosmetic components varies depending on the intended use of the cosmetic and the like, and thus cannot be determined unconditionally, but is preferably adjusted appropriately according to the application.

The form of the anti-aging cosmetic composition of the present invention is optional and not limited, but it has excellent properties such as preventing skin aging and maintaining a youthful and healthy skin condition. Examples include basic cosmetics such as creams, emulsions, lotions, essences, facial cleansers, packs, etc., makeup cosmetics such as lipsticks, foundations, liquid foundations, press powders, and toiletries such as body soaps and soaps.

The anti-aging cosmetic composition of the present invention acts on hair roots and hair, and is also effective in preventing split ends and hair breakage and protecting hair. For example, shampoos, rinses, treatments, conditioners, hair conditioners Such as hair products.

Furthermore, when the above-mentioned decomposed product and its dry powder are put into hot water, it is effective in maintaining the feeling of warmth after bathing and improving the condition of the skin by the effect of improving the variation in the area between keratinocytes. Therefore, the anti-aging cosmetic of the present invention can also be used as a bath product such as a bath agent.
As described above, when the anti-aging cosmetic composition of the present invention is used as a bath agent, the amount of the decomposed product in the cosmetic is 0.0005 to 5.0 parts, preferably 100 parts by weight, in terms of the solid content of the decomposed product per 100 parts of the cosmetic. 0.001 to 0.1 part. When the above-mentioned bath agent is used, the amount of the bath agent is preferably adjusted so that the amount of the bath agent is usually about 25 g per 200 liters of hot water.

Next, the anti-aging cosmetic composition of the present invention will be described in more detail based on examples, but the present invention is not limited to these examples.

Preparation Example 1 (Production of Enzymatic Hydrolyzate of Rice Alkaline Extract) 100 g of ground rice is immersed in 400 ml of water adjusted to pH 11 to 13 with sodium hydroxide at room temperature for about 24 hours. ,
Extracted.

Thereafter, the extract was adjusted to the optimum pH of each enzyme to be used and treated with actinase, pepsin and trypsin in an amount of 5 mg each. The processing conditions were all 30 to 40 ° C. for 1 to 2 hours.

This was filtered to give a pale yellow transparent enzyme decomposition product solution (solid content: about 0.5% by weight, weight average molecular weight: 2500)
Below) about 250ml was obtained.

Preparation Example 2 (Production of Enzymatic Hydrolyzate of Rice Alkaline Extract) A pale yellow transparent enzymatic hydrolyzate solution was prepared in the same manner as in Preparation Example 1 except that papain was used instead of pepsin used in Preparation Example 1. Solid content: about 0.5% by weight, weight average molecular weight: 2500
Below) about 250ml was obtained.

Preparation Example 3 (Production of Enzymatically Decomposed Product of Rice Bran Alkaline Extract) 200 g of rice bran was immersed in 800 ml of water adjusted to pH 11 to 13 with sodium hydroxide at room temperature for about 24 hours to extract. .

Thereafter, the extract was adjusted to the optimum pH of each enzyme to be used, and treated with 10 mg of actinase, pepsin and trypsin each. The processing conditions were all 30 to 40 ° C. for 1 to 2 hours.

This was filtered to give a pale yellow transparent enzyme decomposition product solution (solid content: about 1.0% by weight, weight average molecular weight: 2500)
Below) about 300ml was obtained.

Preparation Example 4 (Production of freeze-dried product of enzymatically decomposed product of rice alkali extract) 100 g of the enzymatically decomposed product solution obtained in Preparative Example 1 was concentrated, and then freeze-dried in a vacuum to dry the decomposed product. About 0.5 g of powder (weight average molecular weight: 2500 or less) was obtained.

[0043]

Preparation Example 5 (Production of spray-dried product of enzymatically decomposed product of rice alkali extract) 250 g of the enzymatically decomposed product solution obtained in Preparative Example 2 was spray-dried to obtain a dry powder of the decomposed product (weight average). Molecular weight: 2500
Approximately 1.25 g was obtained.

Test Examples 1 to 4 The following tests were carried out using the enzyme hydrolyzate solution obtained in Preparation Example 1 as a sample.

Test Example 1 (Lipid peroxide production inhibitory action) 1.0 ml of 0.5 M linoleic acid ethanol, 0.2 M phosphate buffer (p
H7.0) 10 ml and ethanol 9.0 ml were each accurately weighed and thoroughly stirred in a stoppered Erlenmeyer flask. To this solution, 5.0 ml of the above-mentioned decomposition product solution accurately weighed was added, followed by sufficient stirring. Immediately after preparation of this solution and 7
0.1 ml each was accurately weighed for each of those left standing for a day, and 4.7 ml of 75% ethanol, 0.1 ml of a 30% ammonium thiocyanate solution, and 0.02 M ferrous chloride.
Add 0.1 ml of 5% hydrochloric acid solution, mix well and stir.
The absorbance at OD.500 nm after exactly 3 minutes was measured, and the peroxide value index was determined from the following equation.

[0047]

(Equation 1)

(Where T ₇ is the absorbance of the solution to which the decomposed product solution was added after 7 days from the start of the test, and B ₇ was the purified water added instead of the decomposed product solution after 7 days from the start of the test.) Absorbance of the solution subjected to the test, T ₀ indicates the absorbance of the solution to which the decomposed product solution was added immediately after the start of the test, and B ₀ indicates the absorbance of the solution to which purified water was added instead of the decomposed product solution immediately after the start of the test.) In addition, the same operation using purified water instead of the decomposition product solution was used as a blank. Table 1 shows the results.

[0049]

[Table 1]

Test Example 2 (SOD-like activity) 0.15 ml of 1 M Tris-HCl buffer, 0.30 ml of 1 mM ethylenediaminetetraacetic acid (EDTA) tetrasodium salt solution, 0.2 m
0.20 ml of M cytochrome C solution, 1 mM xanthine solution
30 ml of each was accurately weighed and thoroughly stirred in a stoppered Erlenmeyer flask. 0.10 ml of the decomposed product solution accurately weighed in this solution and a xanthine oxidase solution (0.01 U / 0.
01 ml) After adding 0.05 ml and 1.90 ml of purified water, OD.550 nm
The absorbance at every 60 seconds was measured.

A blank similarly operated using purified water instead of the decomposition product solution was used. These results are shown in FIG.

Test Example 3 (Collagen synthesis promoting action) Eagle minimum essential medium (MEM) containing 5% FCS was used as a basic medium, and human dermis-derived fibroblasts were used as cells. After culturing the cells in a basal medium for a certain period, a medium containing 5% of the above-mentioned degradation solution was added, and the cells were cultured for 5 days. The medium was exchanged once during that time, and the cells were cultured using only the basal medium as a blank.

With respect to the fibroblasts cultured by the above operation, the effect of promoting the synthesis of collagen was examined.

The amount of collagen was determined using a collagen stain kit from Cosmo Bio. These results are shown in FIG.

Test Example 4 (Effect of recovery from ultraviolet damage) Eagle MEM containing 10% FCS was used as a basic medium, and human-derived fibroblasts were used as cells. After culturing the cells in a basic medium for a certain period of time, the cells were irradiated with ultraviolet rays (Toshiba Health Line Lamp, manufactured by Toshiba Corp., output 30 w), a medium containing 10% of the decomposition solution was added, and the cells were cultured for 10 days. The medium was exchanged twice during that time, and the cells were cultured using only the basal medium as a blank.

For the fibroblasts cultured by the above operation, the cells were detached after culturing using trypsin EDTA, the number of cells was measured, and the effect of recovering damage caused by ultraviolet rays was examined. These results are shown in FIG.

Formulation Examples 1 to 13 and Comparative Formulation Examples 1 to 13 Formulation Example 1 (Cream) [Component (A)] (parts) Liquid paraffin 5.0 hexalane (glyceryl trioctanoate, manufactured by Kyoei Chemical Industry Co., Ltd.) 4.0 paraffin 5.0 Cetanol 2.0 Glyceryl monostearate 2.0 Polyoxyethylene (20) sorbitan monostearate 6.0 Butyl paraben 0.1 [Component (B)] Decomposed product solution obtained in Preparation Example 1 20.0 Glycerin 5.0 Sodium carboxymethyl cellulose 0.1 Methyl paraben 0.1 Moiston C ( (Natural Moisturizing Factor, manufactured by Kyoei Chemical Industry Co., Ltd.) 1.0 Purified water Amount that total amount becomes 100.0 parts [Component (C)] Fragrance 0.3 After heating the above components (A) and (B) to 80 ° C. or higher, respectively. , (A) and (B) were mixed and stirred. This is 50
After cooling to ° C., the component (C) was added, and the mixture was further stirred and mixed to prepare a uniform cream.

Formulation Example 2 (Emulsion) [Component (A)] (parts) Liquid paraffin 6.00 hexalane (glyceryl trioctanoate, manufactured by Kyoei Chemical Industry Co., Ltd.) 4.00 jojoba oil 1.00 polyoxyethylene (20) sorbitan monostearate 2.00 Soybean lecithin oil 1.50 Methylparaben 0.15 Ethylparaben 0.03 [Component (B)] Decomposed product solution obtained in Preparation Example 1 30.00 Glycerin 3.00 1,3-butylene glycol 2.00 Sodium carboxymethylcellulose 0.30 Sodium hyaluronate 0.01 Purified water The total amount is 100.00 parts. Amount [Component (C)] Fragrance 0.05 After the components (A) and (B) were each heated to 80 ° C., the components (A) and (B) were mixed and stirred. After cooling to 50 ° C., the above-mentioned component (C) was added and further stirred to prepare a uniform emulsion.

Formulation Example 3 (Lotion) [Component] (parts) L-ascorbyl magnesium phosphate 2.0 ethanol 10.0 glycerin 3.0 1,3-butylene glycol 2.0 methyl paraben 0.2 citric acid 0.1 sodium citrate 0.3 carboxyvinyl polymer 0.1 water-soluble placenta Extract 1.0 Decomposed product solution obtained in Preparation Example 10.0 10.0 Perfume trace amount Purified water Amount such that total amount becomes 100.0 parts The above components were mixed to prepare a uniform lotion.

Formulation Example 4 (Essence) [Component] (parts) Ethanol 10.0 Glycerin 5.0 1,3-butylene glycol 1.0 Oleyl alcohol 0.1 Polyoxyethylene sorbitan monolaurate (20EO) 1.0 Methyl paraben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.3 Decomposed product solution obtained in Preparation Example 1.0 Fragrance trace amount Water-soluble collagen 1.0 Purified water Amount that total amount becomes 100.0 parts The above components were mixed to prepare a uniform essence.

Formulation Example 5 (pack) [Component] (parts) Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.1 Decomposed product solution obtained in Preparation Example 2 10.0 Perfume trace amount Purified water The total amount was 100.0 parts. Amounts were mixed to make a uniform pack.

Formulation Example 6 (face wash) [Component] (parts) Stearic acid 15.0 Lauric acid 5.0 Myristic acid 15.0 Glyceryl monostearate 4.0 Potassium hydroxide 7.0 Glycerin 8.0 Decomposed product solution obtained in Preparation Example 3 10.0 Methylparaben 0.2 The amount of purified water to be 100.0 parts was heated to 85 ° C and mixed to prepare a uniform face wash.

Formulation Example 7 (Shampoo) [Component] (parts) Polyoxyethylene lauryl ether sodium sulfate 25.0 lauryl sulfate triethanolamine 15.0 coconut oil fatty acid diethanolamide 4.0 ethylene glycol monostearate 1.50 disodium edetate 0.05 methyl paraben 0.10 Fragrance trace amount Yellow No. 4 trace amount Decomposed product solution obtained in Preparation Example 2 50.0 Purified water Amount of the total amount to be 100.00 parts After the above components were heated to 80 ° C. or higher, they were mixed and stirred to prepare a uniform shampoo.

Formulation Example 8 (rinse) [Component] (parts) Stearyl trimethylammonium hydrochloride 1.5 cetanol 2.0 2-octyldodecanol 1.0 cationized cellulose 0.5 polyoxyethylene cetyl ether 1.0 propylene glycol 5.0 methyl paraben 0.1 fragrance trace amount In Preparation Example 3 The obtained decomposed product solution 50.0 Purified water An amount that would make the total amount 100.0 parts The above components were heated to 80 ° C or higher, and then mixed and stirred to prepare a uniform rinse.

Formulation Example 9 (Treatment) [Ingredients] (parts) Stearyltrimethylammonium chloride 6.0 Polyvinylpyrrolidone 4.0 Glycerin 1.0 Ethylparaben 0.1 Decomposed product solution obtained in Preparation Example 5 After heating the components to 80 ° C., they were mixed and stirred to prepare a uniform treatment.

Formulation Example 10 (Hairdressing agent) [Component] (parts) Ethanol 60.0 Polyoxypropylene butyl ether (40EO) 20.0 Diisopropanolamine 2.0 Edetate Trace amount of fragrance Trace amount Decomposed product solution obtained in Preparation Example 2 was 100.0% Parts Amount The above components were mixed to prepare a uniform hairdressing composition.

Formulation Example 11 (bath agent) [Component] (parts) Sodium sulfate 35.0 Sodium bicarbonate 52.0 Borax 2.0 Sodium carboxymethylcellulose 1.0 Red No. 201 Trace amount of fragrance Trace amount of dry powder of decomposed product obtained in Preparation Example 5 The above components were mixed to prepare a uniform bath agent.

Formulation Example 12 (lipstick) [Component (A)] (parts) Castor oil 50.0 octyldodecanol 5.0 lanolin 5.0 liquid lanolin 5.0 beeswax 4.0 ozokerite 7.0 candelilla wax 2.0 carnauba wax 1.0 [component (B)] Titanium oxide 1.0 pigment (Red No. 201, etc.) Total 4.0 Amount of the dried powder of the decomposed product obtained in Preparation Example 4 to be 100.0 parts in total [Component (C)] A trace amount of fragrance The components (A) and (B) were heated individually. Later
The components (A) and (B) were mixed and stirred. The mixture was re-heated, the component (C) was added, the mixture was poured into a mold and rapidly cooled to prepare a lipstick.

Formulation Example 13 (Liquid Foundation) [Component (A)] (parts) Stearic acid 2.4 Propylene glycol monostearate 2.0 Cetostearyl alcohol 0.2 Liquid lanolin 2.0 Liquid paraffin 3.0 Isopropyl myristate 8.5 Propylparaben [Amount] (Component (B)) The amount of the decomposed product solution obtained in Preparation Example 2 to be 100.0 parts The amount of sodium carboxymethylcellulose 0.2 Bentonite 0.5 Propylene glycol 4.0 Triethanolamine 1.1 Methyl paraben Suitable amount [Component (C)] Titanium oxide 8.0 Talc 4.0 Color pigment After heating the appropriate amount of each of the components (A) and (B),
The components (A) and (B) were mixed and stirred. The mixture was heated again, the component (C) was added, the mixture was poured into a mold, and the mixture was stirred until the temperature reached room temperature to prepare a liquid foundation.

Comparative Formulation Example 1 (Cream) A cream was prepared in the same manner as in Formulation Example 1 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 1.

Comparative Formulation Example 2 (Emulsion) An emulsion was prepared in the same manner as in Formulation Example 2 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 1.

Comparative Formulation Example 3 (Lotion) A lotion was prepared in the same manner as in Formulation Example 3 except that purified water was used in place of the decomposition product solution obtained in Preparation Example 1.

Comparative Formulation Example 4 (Essence) An essence was prepared in the same manner as in Formulation Example 4 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 1.

Comparative Formulation Example 5 (Pack) A pack was prepared in the same manner as in Formulation Example 5, except that purified water was used instead of the decomposed product solution obtained in Preparation Example 2.

Comparative Formulation Example 6 (Face Wash) A face wash was prepared in the same manner as in Formulation Example 6, except that purified water was used instead of the decomposition product solution obtained in Preparation Example 3.

Comparative Formulation Example 7 (Shampoo) A shampoo was prepared in the same manner as in Formulation Example 7 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 2.

Comparative Formulation Example 8 (Rinse) A rinse was prepared in the same manner as in Formulation Example 8 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 3.

Comparative Formulation Example 9 (Treatment) A treatment was prepared in the same manner as in Formulation Example 9 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 3.

Comparative Formulation Example 10 (Hairdressing Agent) A hairdressing agent was prepared in the same manner as in Preparation Example 10 except that purified water was used instead of the decomposed product solution obtained in Preparation Example 2.

Comparative Formulation Example 11 (Bath) A bath formulation was prepared in the same manner as in Formulation Example 11, except that sodium sulfate was used instead of the dry powder of the decomposed product obtained in Preparation Example 5.

Comparative Formulation Example 12 (Lipstick) A lipstick was prepared in the same manner as in Formulation Example 12, except that nylon powder was used instead of the dry powder of the decomposed product obtained in Preparation Example 4.

Comparative Formulation Example 13 (Liquid Foundation) A liquid foundation was prepared in the same manner as in Formulation Example 13, except that purified water was used instead of the decomposed product solution obtained in Preparation Example 2.

Example 1 The cosmetics obtained in Formulation Examples 1 to 6 and Comparative Formulation Examples 1 to 6 were respectively subjected to the following monitor tests. Table 2 shows the results.

(Monitor test) Age randomly selected
For 100 women aged 25 to 57 years, after applying each cosmetic to the facial skin for one month every day, the effect of improving skin dullness and spots, the effect of improving fine wrinkles, and the area between keratinocytes The reduction effect on variation was evaluated based on the following criteria.

[Improvement effect on skin dullness and spots] A: Extremely improved B: Somehow improved C: Not changed D: Slightly noticeable E: Increased noticeability [Effect on fine wrinkles] A : Clearly disappeared B: Somehow inconspicuous C: Not changed D: Somehow increased E: Increased [Reduction effect on variation in the area between keratinocytes] Double-sided tape (30 cells on the left cheek of the face of the panelist) Using a Nystack (manufactured by Nichiban Co., Ltd.), the keratinocytes were separated by a tape stripping method, and the area of each cell was measured. The standard deviation was calculated for the obtained cell area and evaluated according to the following criteria.

A: Standard deviation decreased by 50% B: Standard deviation decreased by 25% C: No change D: Standard deviation increased by 25% E: Standard deviation increased by 50% No test showed any abnormalities on the skin.

[0087]

[Table 2]

Example 2 The head cosmetics obtained in Formulation Examples 7 to 10 and Comparative Formulation Examples 7 to 10 were subjected to the following half head tests. Table 3 shows the results.

(Half-Head Test) The hair split ends after using each hair cosmetic once a day for 30 days for 20 randomly selected women of 25 to 60 years of age , Stiffness, and gloss were evaluated based on the following criteria.

[Split ends] A: lost B: reduced somewhat C: not changed D: increased somehow E: increased [Koshi] A: increased B: somewhat increased C: not changed D: Somehow weakened E: Weakened [gloss] A: Improved B: Somehow improved C: No change D: Somehow creased E: Cranked As a result of the half head test, it was found on the hair and scalp No one complained of the anomaly.

[0091]

[Table 3]

Example 3 The bath preparations obtained in Formulation Example 11 and Comparative Formulation Example 11 were
The following monitor tests were respectively performed. Table 4 shows the results.

(Monitor test) Age randomly extracted
For 20 men and women aged 27 to 59 years, each bath agent was used for 4 weeks, and the reduction effect on the variation in the area between keratinocytes as the skin condition was evaluated based on the following criteria. The bath was dissolved by adding 25 g to 200 liters of hot water.

[Reducing Effect on Area Variation Between Keratinocytes] Except for using 30 cells on the left flank of the panel,
Evaluation was performed in the same manner as in Example 1.

A: Standard deviation decreased by 50% B: Standard deviation decreased by 25% C: No change D: Standard deviation increased by 25% E: Standard deviation increased by 50% No test showed any abnormalities on the skin.

[0096]

[Table 4]

Example 4 The cosmetics obtained in Formulation Examples 12 to 13 and Comparative Formulation Examples 12 to 13 were subjected to the following monitor tests. Table 5 shows the results.

(Monitor test) Age randomly selected
For 100 women aged 25 to 57 years, after applying each cosmetic on facial cheek skin for one month every day, improvement effect on fine wrinkles, daily use on lips or facial cheek skin for one month After that, the effect of reducing the variation in the area between keratinocytes was evaluated based on the following criteria.

[Effect on fine wrinkles] A: Clearly disappeared B: Somehow less noticeable C: Not changed D: Somehow increased E: Increased [Reduction effect on variation in area between keratinocytes] Prescription example
The cosmetics of 12 and Comparative Formulation Example 12 are the cells of the lips of the panelist 30
The cosmetics of Formulation Example 13 and Comparative Formulation Example 13 were evaluated in the same manner as in Example 1 using 30 cells on the left cheek of the face of the panelist.

A: Standard deviation decreased by 50% B: Standard deviation decreased by 25% C: No change D: Standard deviation increased by 25% E: Standard deviation increased by 50% No test showed any abnormalities on the skin.

[0101]

[Table 5]

[0102]

Rice and / or alkaline extract the degradation product to be E by treatment with multiple proteolytic enzymes rice bran used in the present invention according to the present invention is indicative of anti-aging effects of the skin has been required conventionally The anti-aging cosmetic composition of the present invention containing the decomposition product has a mere moisturizing effect because it has effects such as a lipid peroxide production inhibitory effect, an SOD-like activation effect, a collagen synthesis promoting effect, and an ultraviolet damage recovery effect. Instead of normal, turnover is normal to human skin, reduces the variation in the area between keratinous cells, prevents dullness and spots on the skin, and improves wrinkles by activating skin cells It has a wonderful effect. In addition, in the case of a hair cosmetic has an effect to improve hair of split ends, Kosi, the tree ya.

[Brief description of the drawings]

FIG. 1 is a graph showing the absorbance at OD.550 nm every 60 seconds of a sample containing a decomposition product solution obtained in Preparation Example 1 and a blank.

[Figure 2] of the sample and the blank containing the hydrolyzate solution was caught in Preparation Example 1 is a graph showing the amount of collagen and non-collagen content of the cells 10 per ^four.

FIG. 3 is a graph showing the number of cells of a sample containing a degradation product solution obtained in Preparation Example 1 and a blank, and the number of resuscitated cells.

Continuation of front page (58) Field surveyed (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (3)

(57) [Claims] 1. A rice and / or anti-aging cosmetic composition, which comprises mixing the alkaline extract decomposition product that is E is treated with two or more proteolytic enzymes rice bran. 2. The method according to claim 1, wherein the protease is an actinase or a pepsi.
, Trypsin, papain, peptidase and
And two or more selected from the group consisting of
The anti-aging cosmetic composition according to claim 1. 3. A proteolytic enzyme, and (A) and Actinase, (B) pepsin acids, trypsin compounds, papain acids, peptidase acids and one proteolytic enzyme selected from the group consisting of bromelain or two or more The anti-aging cosmetic composition according to claim 1, which is a combination of: