JP2000264834A - Bleaching cosmetic - Google Patents
Bleaching cosmeticInfo
- Publication number
- JP2000264834A JP2000264834A JP11112728A JP11272899A JP2000264834A JP 2000264834 A JP2000264834 A JP 2000264834A JP 11112728 A JP11112728 A JP 11112728A JP 11272899 A JP11272899 A JP 11272899A JP 2000264834 A JP2000264834 A JP 2000264834A
- Authority
- JP
- Japan
- Prior art keywords
- rice bran
- extract
- acid
- extraction
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
Landscapes
- Cosmetics (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、すぐれた美白効果
を有し、しかも皮膚に対する安全性の高い美白化粧料に
関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic which has an excellent whitening effect and is highly safe for the skin.
【0002】[0002]
【従来の技術】日焼け或いは加齢に伴って生ずる色素沈
着、特にシミ、ソバカスの予防、症状改善を目的とし
て、従来よりコウジ酸、アスコルビン酸誘導体、ハイド
ロキノン誘導体など種々の美白剤が提案され、それらを
配合した美白化粧料が上市されている。しかしながら、
それら従来の美白剤は、美白効果それ自体が必ずしも十
分満足し得るものではないことに加えて、美白効果を最
大限に発揮させるためその配合量を増やすと、皮膚刺激
の問題を生ずることがあるなど、実用性の面でなお改善
すべき点が多い。2. Description of the Related Art Various whitening agents such as kojic acid, ascorbic acid derivatives and hydroquinone derivatives have been conventionally proposed for the purpose of preventing pigmentation caused by sunburn or aging, especially spots and freckles, and improving symptoms. Is marketed. However,
These conventional whitening agents, in addition to the whitening effect itself cannot always be sufficiently satisfactory, may cause skin irritation problems when the amount thereof is increased to maximize the whitening effect. There are many points that need to be improved in terms of practicality.
【0003】[0003]
【発明が解決しようとする課題】本発明者らは、かかる
従来技術の問題点に鑑み、美白効果にすぐれると共に皮
膚に対する安全性の高い美白化粧料を提供ずべく、天然
物中の美白成分の探索とその化粧料への応用について鋭
意研究を進めた結果、米糠の特定の抽出画分がチロシナ
ーセ活性抑制作用に基づくすぐれた美白効果を有し、又
天然物由来であるため皮膚刺激性も少ないことを見い出
し、本発明を完成するに至った。DISCLOSURE OF THE INVENTION In view of the problems of the prior art, the present inventors have attempted to provide a whitening component in natural products in order to provide a whitening cosmetic which has an excellent whitening effect and is highly safe for the skin. As a result of intensive research on the search for and application of it to cosmetics, the specific extract fraction of rice bran has an excellent whitening effect based on the tyrosinase activity inhibitory effect, and also has skin irritation due to its natural origin. They have found that there are few, and have completed the present invention.
【0004】[0004]
【課題を解決するための手段】即ち、本発明は、米糠を
酸性水性媒体で抽出して得られる抽出物を配合したこと
を特徴とする美白化粧料である。That is, the present invention is a whitening cosmetic comprising an extract obtained by extracting rice bran with an acidic aqueous medium.
【0005】以下、本発明について詳細に説明する。本
発明にいう米糠としては、玄米を精米する際に得られる
所謂米糠のほか、玄米それ自体を使用することも可能で
あり、要はその全部又は一部が米糠相当成分からなるも
のであればよく、その種類に特に制限はない。Hereinafter, the present invention will be described in detail. As the rice bran referred to in the present invention, besides the so-called rice bran obtained when milling brown rice, it is also possible to use brown rice itself, that is, if all or a part thereof is composed of a component equivalent to rice bran. Well, there is no particular limitation on the type.
【0006】米糠の抽出に用いる酸性水性媒体として
は、一般には水を酸でpH5以下、好ましくは1〜4に
調整したものが使用される。水に代えて、水と水混和性
有機溶媒との混合物を用いることもできる。[0006] As the acidic aqueous medium used for extraction of rice bran, generally, water adjusted to pH 5 or less, preferably 1 to 4 with acid is used. Instead of water, a mixture of water and a water-miscible organic solvent can be used.
【0007】水性媒体のpH調整に用いる酸としては、
塩酸、硫酸、燐酸などの無機酸、酢酸、乳酸、リンゴ
酸、クエン酸などの有機酸がある。それらのうちでも、
特に乳酸の使用が好ましい。又、水混和性有機溶媒を併
用する場合、該溶媒としては、メタノール、エタノー
ル、プロパノールなどの一価アルコール類、エチレング
リコール、プロピレングリコール、1.3−ブチレング
リコール、ジエチレングリコール、グリセリンなどの多
価アルコール類、酢酸エチル、酢酸ブチル、プロピオン
酸メチルなどのエステル類、アセトン、メチルエチルケ
トン、ジオキサンなどのケトン類等を挙げることができ
る。水に対するそれら有機溶媒の混合割合は、一般には
70重量%以下、特に50重量%以下が望ましい。The acids used for adjusting the pH of the aqueous medium include:
There are inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid, and organic acids such as acetic acid, lactic acid, malic acid and citric acid. Among them,
Particularly, use of lactic acid is preferred. When a water-miscible organic solvent is used in combination, examples of the solvent include monohydric alcohols such as methanol, ethanol, and propanol; and polyhydric alcohols such as ethylene glycol, propylene glycol, 1.3-butylene glycol, diethylene glycol, and glycerin. And esters such as ethyl acetate, butyl acetate and methyl propionate, and ketones such as acetone, methyl ethyl ketone and dioxane. The mixing ratio of these organic solvents to water is generally 70% by weight or less, preferably 50% by weight or less.
【0008】かかる酸性水性媒体を用いる米糠抽出物の
調製方法を、例えば被抽出物として通常の米糠を用いる
場合について述べれば、以下の通りである。即ち、まず
米糠に、必要ならば加熱等による酵素失活処理さらには
脱脂処理等を施した後、その乾燥重量に対して、通常1
〜10倍量、好ましくは2〜5倍量の酸性水性媒体を加
え、十分混合した上、攪拌下もしくは無攪拌下に抽出を
行う。抽出条件としては、用いる溶媒の組成、pH等に
よって異なるが、一般には4〜40℃で6時間〜7日程
度である。A method for preparing a rice bran extract using such an acidic aqueous medium, for example, in the case of using ordinary rice bran as an extract, is as follows. That is, first, rice bran is subjected to an enzyme inactivation treatment and a degreasing treatment by heating or the like, if necessary.
Add 10 to 10 times, preferably 2 to 5 times the amount of acidic aqueous medium, mix well, and extract with stirring or without stirring. The extraction conditions vary depending on the composition of the solvent used, pH, etc., but are generally at 4 to 40 ° C. for about 6 hours to 7 days.
【0009】抽出を終わったならば、次に濾過、遠心分
離等の固液分離操作を施して固形物を除去した後、ここ
に得られる抽出液を、一般にはさらに、通常美白化粧料
で用いられるpH領域であるpH6.0〜8.0の範囲
に調整し、析出物を濾過等によって除去して米糠抽出物
を得る。After completion of the extraction, the solid is removed by performing a solid-liquid separation operation such as filtration and centrifugation, and the extract obtained here is generally further used in a whitening cosmetic. The pH is adjusted to the range of 6.0 to 8.0, which is the pH range obtained, and the precipitate is removed by filtration or the like to obtain a rice bran extract.
【0010】上記の工程中、例えば固形物除去後の抽出
液に、蛋白分解酵素等による酵素処理を施してもよく、
これによって米糠抽出物の保存安定性を高めることがで
きる。[0010] In the above-mentioned step, for example, the extract after removing solids may be subjected to an enzyme treatment with a protease or the like.
Thereby, the storage stability of the rice bran extract can be enhanced.
【0011】かくして得られる米糠抽出物は、そのま
ま、もしくはさらに減圧濃縮等により適宜の濃度に調整
して、美白化粧料中に配合するか、場合によっては、ス
プレードライ法、凍結乾燥法など常法に従って粉末化し
た上、該化粧料中に配合される。The rice bran extract thus obtained is used as it is, or further adjusted to an appropriate concentration by concentration under reduced pressure or the like, and blended into a whitening cosmetic, or in some cases, a conventional method such as a spray drying method or a freeze drying method. And then blended into the cosmetic.
【0012】本発明の美白化粧料中に於ける上記の米糠
抽出物の配合量は、用いた米糠原料等によっても異なる
が、通常の米糠を使用した場合であれば、固形分として
一般に0.002〜2重量%、好ましくは0.01〜
0.5重量%の範囲である。配合量が0.002重量%
を下回ると、所望の美白効果を得ることが困難となり、
一方2重量%を越えて多く配合しても、配合量に見合っ
たそれ以上の美白効果の向上は期待し難く、コスト、配
合操作等の面で問題を生ずるのみで好ましくない。The amount of the above-mentioned rice bran extract in the whitening cosmetic of the present invention varies depending on the rice bran raw material used and the like. However, when ordinary rice bran is used, it is generally 0.1% as a solid content. 002 to 2% by weight, preferably 0.01 to
It is in the range of 0.5% by weight. 0.002% by weight
Below, it becomes difficult to obtain the desired whitening effect,
On the other hand, if the amount is more than 2% by weight, it is difficult to expect a further improvement in the whitening effect in proportion to the amount of the compounding.
【0013】米糠原料として玄米等を用いて得られる抽
出物を化粧料に配合する場合は、用いた原料中の米糠含
量或いは抽出物の美白効果等を勘案して、配合量が調整
される。When an extract obtained by using brown rice or the like as a rice bran raw material is incorporated into a cosmetic, the amount is adjusted in consideration of the rice bran content in the raw material used or the whitening effect of the extract.
【0014】本発明の美白化粧料には、必須成分の米糠
抽出物の他に、通常化粧料に用いられる配合成分である
油脂成分、界面活性剤、保湿剤、増粘剤、防腐殺菌剤、
粉体成分、紫外線吸収剤、色素、香料等が必要に応じて
適宜配合され、それら成分によってクリーム、乳液、ロ
ーション、軟膏、パック剤等適宜の剤型とした上使用に
供される。The whitening cosmetic composition of the present invention contains, in addition to the rice bran extract, which is an essential component, oil and fat components, surfactants, moisturizers, thickeners, preservatives and bactericides which are commonly used in cosmetics.
Powder components, ultraviolet absorbers, pigments, fragrances, and the like are appropriately compounded as necessary, and the components are used in the form of creams, emulsions, lotions, ointments, packs, and the like.
【0015】ここで、油脂成分としては、例えばオリー
ブ油、ホホバ油、ヒマシ油、大豆油、ヤシ油、パーム
油、カカオ油、ミンク油等の油脂類:ミツロウ、カルナ
ウバロウ、ラノリン等のロウ類:流動パラフィン、ワセ
リン、パラフィンワックス、スクワラン等の炭化水素
類:ミリスチン酸、パルミチン酸、ステアリン酸、オレ
イン酸、イソステアリン酸等の脂肪酸類:ラウリルアル
コール、セタノール、ステアリルアルコール等の高級ア
ルコール類:ミリスチン酸イソプロピル、パルミチン酸
イソプロピル、オレイン酸ブチル等の合成エステル類が
挙げられる。The fats and oils include, for example, oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, coconut oil, palm oil, cacao oil and mink oil: waxes such as beeswax, carnauba wax and lanolin: fluid Hydrocarbons such as paraffin, petrolatum, paraffin wax and squalane: fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid and isostearic acid: higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol: isopropyl myristate, Synthetic esters such as isopropyl palmitate and butyl oleate are exemplified.
【0016】界面活性剤としては、ポリオキシエチレン
アルキルエーテル、ポリオキシエチレン脂肪酸エステ
ル、ポリオキシエチレンソルビタン脂肪酸エステル、グ
リセリン脂肪酸エステル、ポリグリセリン脂肪酸エステ
ル、ポリオキシエチレングリセリン脂肪酸エステル、ポ
リオキシエチレン硬化ヒマシ油、ポリオキシエチレンソ
ルビトール脂肪酸エステル等の非イオン界面活性剤:脂
肪酸塩、アルキル硫酸塩、アルキルベンゼンスルホン酸
塩、ポリオキシエチレンアルキルエーテル硫酸塩、ポリ
オキシエチレン脂肪アミン硫酸塩、ポリオキシエチレン
アルキルフェニルエーテル硫酸塩、ポリオキシエチレン
アルキルエーテル燐酸塩、α−スルホン化脂肪酸アルキ
ルエステル塩、ポリオキシエチレンアルキルフェニルエ
ーテル燐酸塩等のアニオン界面活性剤:第四級アンモニ
ウム塩、第一級〜第三級脂肪アミン塩、トリアルキルベ
ンジルアンモニウム塩、アルキルビリジニウム塩、2−
アルキル−1−アルキル−1−ヒドロキシエチルイミダ
ゾリニウム塩、N.N−ジアルキルモルフォルニウム
塩、ポリエチレンポリアミン脂肪酸アミド塩等のカチオ
ン界面活性剤:N.N−ジメチル−N−アルキル−N−
カルボキシメチルアンモニオベタイン、N.N.N−ト
リアルキル−N−アルキレンアンモニオカルボキシベタ
イン、N−アシルアミドプロピル−N’N’−ジメチル
−N’−β−ヒドロキシプロビルアンモニオスルホベタ
イン等の両イオン性界面活性剤等が挙げられる。Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene hydrogenated castor oil. , Non-ionic surfactants such as polyoxyethylene sorbitol fatty acid esters: fatty acid salts, alkyl sulfates, alkyl benzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates Salt, polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate, etc. On surfactant: quaternary ammonium salts, primary to tertiary fatty amine salts, trialkyl benzyl ammonium salts, alkyl pyridinium salts, 2-
Alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N.P. Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts: N-dimethyl-N-alkyl-N-
Carboxymethylammoniobetaine, N.P. N. Zwitterionic surfactants such as N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N'N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine, and the like. .
【0017】保湿剤としては、例えばグリセリン、プロ
ピレングリコール、ジプロピレングリコール、1.3−
ブチレングリコール、ポリエチレングリコール、ソルビ
トール、ピロリドンカルボン酸塩、ヒアルロン酸等のム
コボリサッカライド、尿素等がある。Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1.3-
There are mucoboli saccharides such as butylene glycol, polyethylene glycol, sorbitol, pyrrolidone carboxylate, and hyaluronic acid, and urea.
【0018】増粘剤としては、例えばトラガントガム、
アルギン酸ナトリウム、ペクチン、カルボキシメチルセ
ルロースナトリウム、ポリビニルアルコール、カルボキ
シビニルポリマー、ポリアクリル酸及びその誘導体、ゼ
ラチン、ポリビニルピロリドン、ビーガム、メチルポリ
シロキサン、デキストラン等がある。Examples of the thickener include tragacanth gum,
Examples include sodium alginate, pectin, sodium carboxymethylcellulose, polyvinyl alcohol, carboxyvinyl polymer, polyacrylic acid and its derivatives, gelatin, polyvinylpyrrolidone, veegum, methylpolysiloxane, and dextran.
【0019】防腐殺菌剤としては、例えば尿素、パラオ
キシ安息香酸メチル、パラオキシ安息香酸エチル、パラ
オキシ安息香酸プロピル、パラオキシ安息香酸ブチル、
フェノキシエタノール、ジクロロフェン、ヘキサクロロ
フェン、塩酸クロルヘキシジン、塩化ベンザルコニウ
ム、サリチル酸、エタノール等がある。Examples of the antiseptic disinfectant include urea, methyl parahydroxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate,
Phenoxyethanol, dichlorophen, hexachlorophen, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, ethanol and the like.
【0020】粉体成分としては、例えば酸化チタン、タ
ルク、カオリン、ベントナイト、酸化亜鉛、炭酸マグネ
シウム、酸化マグネシウム、酸化ジルコニウム、硫酸バ
リウム、無水ケイ酸、雲母、セリサイト、ナイロンパウ
ダー、シルクパウダー等がある。Examples of the powder component include titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, sericite, nylon powder, silk powder and the like. is there.
【0021】紫外線吸収剤としては、例えばパラアミノ
安息香酸エチル、パラジメチルアミノ安息香酸エチルヘ
キシル、サリチル酸アミル、パラメトキシ桂皮酸エチル
ヘキシル、桂皮酸オクチル、2.4−ジヒドロキシベン
ゾフェノン、2−ヒドロキシ−4−メトキシベンゾフェ
ノン−5−スルホン酸塩、2−(2−ヒドロキシ−5−
メチルフェニル)ベンゾトリアゾール、ウロカニン酸、
ウロカニン酸エチル等がある。Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate, ethylhexyl paramethoxycinnamate, octyl cinnamate, 2.4-dihydroxybenzophenone, and 2-hydroxy-4-methoxybenzophenone-. 5-sulfonate, 2- (2-hydroxy-5-
Methylphenyl) benzotriazole, urocanic acid,
And ethyl urocanate.
【0022】本発明の美白化粧料には、本発明の米糠抽
出物の有効性を損なわない範囲で、他の美白剤をはじめ
各種化粧料活性成分をさらに配合し、併用することがで
きる。かかるものとしては、例えばコウジ酸及びその誘
導体、アルブチン、アスコルビン酸及びその誘導体(L
−アスコルビン酸−2−燐酸エステル塩、L−アスコル
ビン酸−2−グルコシドなど)、エラグ酸、レゾルシノ
ール誘導体(4−n−ブチルレゾルシノールなど)ソウ
ハクヒ抽出液、ユキノシタ抽出液等の美白剤:ビタミン
E及びその誘導体(ビタミンEニコチネート、ビタミン
Eリノレートなど)、ニコチン酸及びその誘導体(ニコ
チン酸アミド、ニコチン酸ベンジルなど)、α−ヒドロ
キシ酸類(乳酸、クエン酸、α−ヒドロキシオクタン酸
など)、ジイソプロピルアミンジクロロアセテート、γ
−アミノ−β−ヒドロキシ酪酸等の老化防止・肌荒れ改
善剤等が挙げられる。The whitening cosmetic composition of the present invention may further contain other cosmetic active ingredients, including other whitening agents, and may be used in combination, as long as the effectiveness of the rice bran extract of the present invention is not impaired. Such substances include, for example, kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives (L
Whitening agents such as ascorbic acid-2-phosphate ester salt, L-ascorbic acid-2-glucoside), ellagic acid, resorcinol derivatives (such as 4-n-butylresorcinol), soybean extract, and saxifrage extract: vitamin E and Derivatives (vitamin E nicotinate, vitamin E linoleate, etc.), nicotinic acid and its derivatives (nicotinamide, benzyl nicotinate, etc.), α-hydroxy acids (lactic acid, citric acid, α-hydroxyoctanoic acid, etc.), diisopropylamine dichloro Acetate, γ
Anti-aging and skin roughness improving agents such as -amino-β-hydroxybutyric acid.
【0023】以下、製造例、実施例及び試験例を挙げて
本発明をさらに具体的に説明するが、本発明はそれらに
限定されるものではない。なお、以下に於いて、%及び
部はそれぞれ重量%及び重量部を意味する。Hereinafter, the present invention will be described more specifically with reference to Production Examples, Examples, and Test Examples, but the present invention is not limited thereto. In the following,% and parts mean% by weight and parts by weight, respectively.
【0024】製造例1 米糠抽出物溶液の調製 米糠400gに0.1M乳酸水溶液(pH2.6)12
00gを加え、攪拌して米糠と乳酸水溶液を十分混合し
た後、室温に2日間静置した。次に不溶物をろ過で除
き、ろ液のpHを1N苛性ソーダ水溶液で8.5に調整
して生じた不溶物をろ別し、ろ液を乳酸でpH6.5と
して米糠抽出物溶液を得た(収量:約800g、固形分
濃度:4.4%)。Production Example 1 Preparation of Rice Bran Extract Solution To 400 g of rice bran was added a 0.1 M lactic acid aqueous solution (pH 2.6) 12
After adding rice bran and an aqueous lactic acid solution with stirring, the mixture was allowed to stand at room temperature for 2 days. Next, the insoluble matter was removed by filtration, the pH of the filtrate was adjusted to 8.5 with a 1N aqueous sodium hydroxide solution, and the resulting insoluble matter was filtered off. The filtrate was adjusted to pH 6.5 with lactic acid to obtain a rice bran extract solution. (Yield: about 800 g, solids concentration: 4.4%).
【0025】製造例2 米糠抽出物溶液の調製 抽出媒体として、0.1M乳酸水溶液に代えて、10%
エタノール含有0.1M乳酸水溶液(pH2.7)を用
いるほかは、製造例1と同様にして米糠抽出物溶液を得
た(収量:約750g、固形分濃度:5.2%)Preparation Example 2 Preparation of Rice Bran Extract Solution As an extraction medium, 10%
A rice bran extract solution was obtained in the same manner as in Production Example 1 except that an ethanol-containing 0.1 M aqueous lactic acid solution (pH 2.7) was used (yield: about 750 g, solid content concentration: 5.2%).
【0026】製造例3 米糠抽出物溶液の調製 抽出媒体として、0.1M乳酸水溶液に代えて、0.1
M燐酸水溶液(pH1.6)を用いるほかは、製造例1
と同様にして米糠抽出物溶液を得た(収量:約700
g、固形分濃度:4.4%)Production Example 3 Preparation of Rice Bran Extract Solution As an extraction medium, 0.1 M lactic acid aqueous solution was used instead of 0.1 M lactic acid aqueous solution.
Production Example 1 except that an aqueous M phosphoric acid solution (pH 1.6) was used.
A rice bran extract solution was obtained in the same manner as described above (yield: about 700
g, solid content concentration: 4.4%)
【0027】製造例4 米糠抽出物溶液の調製 抽出媒体として、0.1M乳酸水溶液に代えて、0.1
N塩酸(pH1.1)を用いるほかは、製造例1と同様
にして米糠抽出物溶液を得た(収量:約650g、固形
分濃度:4.0%)Production Example 4 Preparation of Rice Bran Extract Solution As an extraction medium, 0.1 M lactic acid aqueous solution was used instead of 0.1 M lactic acid aqueous solution.
A rice bran extract solution was obtained in the same manner as in Production Example 1 except that N hydrochloric acid (pH 1.1) was used (yield: about 650 g, solid content concentration: 4.0%).
【0028】製造例5米糠抽出物溶液の調製 抽出媒体として、0.1M乳酸水溶液に代えて、0.1
Mクエン酸(pH2.3)を用いるほかは、製造例1と
同様にして米糠抽出物溶液を得た(収量:約600g、
固形分濃度:4.8%)Production Example 5 Preparation of Rice Bran Extract Solution As an extraction medium, 0.1 M lactic acid aqueous solution was used instead of 0.1 M lactic acid aqueous solution.
Except for using M citric acid (pH 2.3), a rice bran extract solution was obtained in the same manner as in Production Example 1 (yield: about 600 g,
(Solid concentration: 4.8%)
【0029】製造例6 米糠抽出物溶液の調製 米糠400gに0.1M乳酸水溶液(pH2.6)12
00gを加え、攪拌して米糠と乳酸水溶液を十分混合し
た後、室温に2日間静置した。次に不溶物をろ過で除
き、ろ液のpHを7.5に調整し、蛋白分解酵素20m
gを加えて40℃で2時間酵素処理を行った後、処理液
をろ過し、ろ液のpHを1N苛性ソーダ水溶液で8.5
に調整して生じた不溶物をろ別し、ろ液を乳酸でpH6
−5として米糠抽出物溶液を得た(収量:約750g、
固形分濃度:4.7%)。Production Example 6 Preparation of Rice Bran Extract Solution A solution of 0.1 M lactic acid aqueous solution (pH 2.6) was added to 400 g of rice bran.
After adding rice bran and an aqueous lactic acid solution with stirring, the mixture was allowed to stand at room temperature for 2 days. Next, insoluble materials were removed by filtration, the pH of the filtrate was adjusted to 7.5, and the amount of
g, and the mixture was subjected to an enzyme treatment at 40 ° C. for 2 hours. The treated liquid was filtered, and the pH of the filtrate was adjusted to 8.5 with a 1N aqueous solution of sodium hydroxide.
The resulting insolubles were filtered off and the filtrate was adjusted to pH 6 with lactic acid.
-5 was obtained as a rice bran extract solution (yield: about 750 g,
Solids concentration: 4.7%).
【0030】製造例7 米糠抽出物粉末の調製 製造例1で得られた米糠抽出液100gを減圧濃縮した
後凍結乾燥し、米糠抽出物粉末約4.4gを得た。Preparation Example 7 Preparation of Rice Bran Extract Powder 100 g of the rice bran extract obtained in Preparation Example 1 was concentrated under reduced pressure and freeze-dried to obtain about 4.4 g of rice bran extract powder.
【0031】製造例8 米糠抽出物溶液の調製 製造例1に於いて、米糠に代えて玄米を用いるほかは製
造例1と同様にして米糠抽出物溶液を得た(収量:約1
000g、固形分濃度:0.5%)。Production Example 8 Preparation of Rice Bran Extract Solution A rice bran extract solution was obtained in the same manner as in Production Example 1 except that brown rice was used instead of rice bran (yield: about 1).
000 g, solid content concentration: 0.5%).
【0032】 実施例1 クリーム [A成分] 部 流動パラフィン 5.0 ヘキサラン((株)テクノーブル製 トリオクタン酸グリセリル) 4.0 パラフィン 5.0 グリセリルモノステアレート 2.0 ポリオキシエチレン(20)ソルビタンモノステアレート 6.0 ブチルパラベン 0.1 [B成分] 製造例1で得られた米糠抽出物溶液 5.0 グリセリン 5.0 カルボキシメチルセルロースナトリウム 0.1 メチルパラベン 0.1 モイストン・C((株)テクノーブル製 NMF成分) 1.0 精製水 全量が100部となる量 [C成分] 香料 適量 上記のA成分とB成分をそれぞれ80℃以上に加熱した
後、攪拌混合した。これを50℃まで冷却した後、C成
分を加えてさらに攪拌混合してクリームを調製した。Example 1 Cream [Component A] Part Liquid paraffin 5.0 Hexalan (Glyceryl trioctanoate manufactured by Technoble Co., Ltd.) 4.0 Paraffin 5.0 Glyceryl monostearate 2.0 Polyoxyethylene (20) sorbitan Monostearate 6.0 Butyl paraben 0.1 [Component B] Rice bran extract solution obtained in Production Example 1 5.0 Glycerin 5.0 Sodium carboxymethyl cellulose 0.1 Methyl paraben 0.1 Moiston C (Co., Ltd.) NMF component manufactured by Technoble) 1.0 Purified water Amount to make the total amount 100 parts [Component C] Fragrance Appropriate amount After heating each of the above components A and B to 80 ° C. or higher, they were mixed by stirring. After cooling to 50 ° C., the C component was added and further stirred and mixed to prepare a cream.
【0033】 実施例2 乳液 [A成分] 部 流動パラフィン 6.0 ヘキサラン((株)テクノーブル製 トリオクタン酸グリセリル) 4.0 ホホバ油 1.0 ポリオキシエチレン(20)ソルビタンモノステアレート 2.0 大豆レシチン 1.5 メチルパラベン 0.15 エチルパラベン 0.03 [B成分] 製造例2で得られた米糠抽出物溶液 5.0 グリセリン 3.0 1.3−ブチレングリコール 2.0 カルボキシメチルセルロースナトリウム 0.3 ヒアルロン酸ナトリウム 0.01 精製水 全量が100部となる量 [C成分] 香料 適量 上記のA成分とB成分をそれぞれ80℃以上に加熱した
後、攪拌混合した。これを50℃まで冷却した後、C成
分を加えてさらに攪拌混合し、乳液を調製した。Example 2 Emulsion [Component A] Part Liquid paraffin 6.0 Hexalan (Glyceryl trioctanoate manufactured by Technoble Co., Ltd.) 4.0 Jojoba oil 1.0 Polyoxyethylene (20) sorbitan monostearate 2.0 Soybean lecithin 1.5 Methylparaben 0.15 Ethylparaben 0.03 [Component B] Rice bran extract solution obtained in Production Example 2 5.0 Glycerin 3.0 1.3 Butylene glycol 2.0 Sodium carboxymethylcellulose 0. 3 Sodium hyaluronate 0.01 Purified water Amount to make the total amount 100 parts [Component C] Perfume Appropriate amount After heating each of the above components A and B to 80 ° C. or higher, they were mixed by stirring. After cooling this to 50 ° C., the C component was added and further stirred and mixed to prepare an emulsion.
【0034】 実施例3 ローション [成分] 部 製造例1で得られた米糠抽出物溶液 5.0 エタノール 10.0 グリセリン 3.0 1.3−ブチレングリコール 2.0 メチルパラベン 0.2 クエン酸 0.1 クエン酸ナトリウム 0.3 カルボキシビニルポリマー 0.1 香料 適量 精製水 全量が100部となる量 上記の成分を混合してローションを調製した。Example 3 Lotion [Component] Part Rice bran extract solution obtained in Production Example 1 5.0 Ethanol 10.0 Glycerin 3.0 1.3 Butylene glycol 2.0 Methylparaben 0.2 Citric acid 1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Perfume Appropriate amount Purified water An amount that gives a total amount of 100 parts The above components were mixed to prepare a lotion.
【0035】 実施例4 パック [成分] 部 ポリビニルアルコール 15.0 ヒドロキシメチルセルロース 5.0 プロピレングリコール 5.0 エタノール 10.0 メチルパラベン 0.2 製造例2で得られた米糠抽出物溶液 3.0 香料 1.0 精製水 適量 全量が100部となる量 上記の成分を攪拌混合してパックを調製した。Example 4 Pack [Components] Part Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.2 Rice bran extract solution obtained in Production Example 2 3.0 Fragrance 1 0.0 Purified water Appropriate amount An amount that makes the total amount 100 parts. The above components were stirred and mixed to prepare a pack.
【0036】 実施例5 洗顔料 [成分] 部 ステアリン酸 15.0 ラウリン酸 5.0 ミリスチン酸 15.0 グリセリルモノステアレート 4.0 水酸化カリウム 7.0 グリセリン 8.0 製造例7で得られた米糠抽出物粉末 0.2 メチルパラベン 0.2 精製水 全量が100部となる量 上記の成分を85℃に加温して攪拌混合し、洗顔料を調
製した。Example 5 Facial Cleanser [Component] Part Stearic Acid 15.0 Lauric Acid 5.0 Myristate 15.0 Glyceryl Monostearate 4.0 Potassium Hydroxide 7.0 Glycerin 8.0 Obtained in Production Example 7 Rice bran extract powder 0.2 Methyl paraben 0.2 Purified water Amount to make the total amount 100 parts The above components were heated to 85 ° C and mixed by stirring to prepare a face wash.
【0037】実施例6 クリーム 実施例1に於いて、製造例1の米糠抽出物溶液に代え
て、製造例4の米糠抽出物溶液を用いるほかは実施例1
と同様にしてクリームを調製した。Example 6 Cream In Example 1, except that the rice bran extract solution of Production Example 4 was used instead of the rice bran extract solution of Production Example 1,
A cream was prepared in the same manner as described above.
【0038】実施例7 乳液 実施例2に於いて、製造例2の米糠抽出物溶液に代え
て、製造例5の米糠抽出物溶液を用いるほかは実施例1
と同様にして乳液を調製した。Example 7 Emulsion Example 1 was repeated except that the rice bran extract solution of Production Example 5 was used in place of the rice bran extract solution of Production Example 2.
An emulsion was prepared in the same manner as described above.
【0039】実施例8 クリーム 実施例1に於いて、製造例1の米糠抽出物溶液に代え
て、製造例7の米糠抽出物溶液を用いるほかは実施例1
と同様にしてクリームを調製した。Example 8 Cream In Example 1, except that the rice bran extract solution of Production Example 7 was used instead of the rice bran extract solution of Production Example 1,
A cream was prepared in the same manner as described above.
【0040】実施例9 クリーム 実施例1に於いて、製造例1の米糠抽出物溶液5.0部
に代えて、該抽出物溶液4.0部とコウジ酸1.0部を
用いるほかは実施例1と同様にしてクリームを調製し
た。Example 9 Cream The procedure of Example 1 was repeated, except that 5.0 parts of the rice bran extract solution of Preparation Example 1 was replaced with 4.0 parts of the extract solution and 1.0 part of kojic acid. A cream was prepared as in Example 1.
【0041】比較例 クリーム 実施例1に於いて、製造例1の米糠抽出物溶液5.0部
に代えてコウジ酸1.0部を用いるほかは実施例1と同
様にしてクリームを調製した。Comparative Example Cream A cream was prepared in the same manner as in Example 1, except that 1.0 part of kojic acid was used instead of 5.0 parts of the rice bran extract solution of Production Example 1.
【0042】対照例 クリーム 実施例1に於いて、製造例1の米糠抽出物溶液を用いな
いほかは実施例1と同様にしてクリームを調製した。Comparative Example Cream A cream was prepared in the same manner as in Example 1, except that the rice bran extract solution of Production Example 1 was not used.
【0043】試験例1 細胞内チロシナーゼ活性抑制作
用 [試験方法]培養B16マウスメラノーマ細胞を、96
穴マイクロプレートに8×103個/穴播種し、10%
仔牛血清(FBS)含有イーグル最少必須培地(ME
M)中、37℃、5%CO2の条件下に1日間プレ培養
した後、培養液を、製造例1の米糠抽出物溶液を1.
0、2.5又は5.0%の濃度となるように添加した1
0%FBS含有イーグルMEM、もしくは製造例2〜5
の米糠抽出物溶液を夫々5.0%の濃度となるように添
加した10%FBS含有イーグルMEMで置換し、同条
件で4日間培養した。次に、培養液を除去し、界面活性
剤(Triton X−100)を添加した細胞処理液
に、5mMのL−ドーパ又は0.2%のMTTを添加し
て37℃でチロシナーゼ反応を行った後、マイクロプレ
ートリーダー(Mode1450、バイオラッド社製)
を用い、波長490nmでドーパ値を又570−630
nmでMTT値をそれぞれ測定した。なお比較のため、
製造例1の米糠抽出物溶液の代わりに、2mMのコウジ
酸を添加した場合及び試料無添加の場合(ブランク)に
ついても、同様の試験を行った。Test Example 1 Inhibition of Intracellular Tyrosinase Activity [Test Method] Cultured B16 mouse melanoma cells were
Seed 8 × 10 3 / well in a well microplate, 10%
Eagle minimum essential medium containing calf serum (FBS) (ME
M), the cells were pre-cultured for 1 day at 37 ° C. and 5% CO 2 , and the culture solution was added to the rice bran extract solution of Production Example 1 in 1.
1 added to a concentration of 0, 2.5 or 5.0%
Eagle MEM containing 0% FBS, or Production Examples 2 to 5
Was replaced with 10% FBS-containing Eagle MEM added to a concentration of 5.0%, respectively, and cultured under the same conditions for 4 days. Next, the culture solution was removed, 5 mM L-dopa or 0.2% MTT was added to the cell treatment solution to which a surfactant (Triton X-100) was added, and a tyrosinase reaction was performed at 37 ° C. Then, a microplate reader (Mode 1450, manufactured by Bio-Rad)
And the dopa value at a wavelength of 490 nm is also 570-630.
MTT values were measured in nm. For comparison,
The same test was performed when 2 mM kojic acid was added instead of the rice bran extract solution of Production Example 1 and when no sample was added (blank).
【0044】[結果]各試料のドーパ値を図1に、又各
試料のMTT値を図2に示す。図1及び図2の結果か
ら、本発明の米糠抽出物が、細胞活性を低下せしめるこ
となく、細胞内チロシナーゼ活性を濃度依存的にかつ有
意に抑制することが判る。[Results] The dopa value of each sample is shown in FIG. 1, and the MTT value of each sample is shown in FIG. From the results shown in FIGS. 1 and 2, it can be seen that the rice bran extract of the present invention significantly inhibits intracellular tyrosinase activity in a concentration-dependent manner without reducing cell activity.
【0045】試験例2 色素沈着抑制試験 本発明の米糠抽出物のin vivo美白作用を、モル
モットを用いた色素沈着試験により調べた。Test Example 2 Pigmentation Inhibition Test The in vivo whitening action of the rice bran extract of the present invention was examined by a pigmentation test using guinea pigs.
【0046】[試料]製造例1の米糠抽出物を精製水で
希釈して固形分濃度0.2%としたものを試料として用
いた。 [試験方法]有色モルモット(雄、8週齢)の背部中央
部のタテ60mm×ヨコ30mmの体毛を剃毛し、該部
分を左右二つに区画した。この区画の一方に試料溶液
を、他方に対照として精製水を、それぞれ朝、夕2回各
5ml宛6日間塗布すると共に、該塗布部位に毎日1回
朝の塗布直前に500mJ/cm2のUV−Bを照射
し、6日目の夕方に照射部位の色素沈着状態を目視によ
り観察し、以下の基準により評価した。[Sample] The rice bran extract of Production Example 1 was diluted with purified water to a solid content of 0.2% and used as a sample. [Test Method] The hair of a colored guinea pig (male, 8 weeks of age) at the center of the back at 60 mm in the vertical direction and 30 mm in the horizontal direction was shaved, and this part was divided into two right and left parts. A sample solution was applied to one of the compartments and purified water as a control was applied to the other side twice each in the morning and evening for 5 days each for 5 ml for 6 days, and once every day immediately before application in the morning at 500 mJ / cm 2 to the application site. -B was irradiated, and in the evening of the sixth day, the pigmentation state of the irradiated site was visually observed and evaluated according to the following criteria.
【0047】 [0047]
【0048】[結果]結果を表1に示す。[Results] The results are shown in Table 1.
【表1】 [Table 1]
【0049】表1に示す通り、本発明の米糠抽出物は紫
外線による皮膚への色素沈着を顕著に防止する効果を有
する。As shown in Table 1, the rice bran extract of the present invention has an effect of remarkably preventing pigmentation on skin due to ultraviolet rays.
【0050】試験例3 皮膚刺激性評価試験 眼粘膜刺激性試験の代替法である培養ウサギ角膜細胞
(SIRC)を用いたニュートラルレッド取り込み試験
により、各種美白剤の皮膚刺激性を評価した。Test Example 3 Skin Irritation Evaluation Test The skin irritation of various whitening agents was evaluated by a neutral red uptake test using cultured rabbit corneal cells (SIRC), which is an alternative to the ocular mucosal irritation test.
【0051】[試料] (1)製造例1の米糠抽出物溶液 (2)コウジ酸 (3)アルブチン[Samples] (1) Rice bran extract solution of Production Example 1 (2) Kojic acid (3) Arbutin
【0052】[試験方法]SIRCを96穴マイクロプ
レートに5000個播種し、5%仔牛血清含有イーグル
最少必須培地(MEM)で3日間培養した後、これに試
料を0.2%の固形分濃度となるように添加し、さらに
2日間培養した。次に、SRICを0.05%ニュート
ラルレッド(NR)含有イーグルMEM培地で3時間培
養し、ここに得られたSRICについでNRの取り込み
率を求め、各試料の皮膚刺激性を評価した。[Test Method] After 5,000 SIRCs were seeded on a 96-well microplate and cultured in Eagle's minimum essential medium (MEM) containing 5% calf serum for 3 days, a sample was added to the solid content concentration of 0.2%. And cultured for 2 days. Next, the SRIC was cultured in an Eagle MEM medium containing 0.05% neutral red (NR) for 3 hours, and the NR uptake rate was determined for the obtained SRIC, and the skin irritation of each sample was evaluated.
【0053】[結果]結果を表2に示す。[Results] The results are shown in Table 2.
【表2】 [Table 2]
【0054】表2に示す通り、本発明の米糠抽出物は、
既存の美白剤に比べて皮膚に対する刺激が少なく、これ
によって安全性のより高い美白化粧料を得ることができ
る。As shown in Table 2, the rice bran extract of the present invention
There is less irritation to the skin as compared to existing whitening agents, which makes it possible to obtain safer whitening cosmetics.
【0055】試験例4 モニターテスト 実施例1、比較例及び対照例の各クリームについて、そ
れらの皮膚色素沈着に対する抑制効果並びに皮膚刺激性
を調べた。 [試験方法]無作為に抽出した年齢18〜50歳の女性
20名を被験者とし、各クリームを1日2回(朝、
晩)、1ヵ月間上背部に塗布し、塗布部の色素沈着の状
態及び皮膚の紅斑を目視で観察し、以下の評価基準に基
づいて評価した。Test Example 4 Monitor Test The creams of Example 1, Comparative Examples and Control Examples were examined for their inhibitory effect on skin pigmentation and skin irritation. [Test method] Twenty women aged 18 to 50 years randomly selected as subjects were given each cream twice a day (morning,
Evening) The composition was applied to the upper back for one month, and the state of pigmentation of the applied part and erythema of the skin were visually observed, and evaluated based on the following evaluation criteria.
【0056】[評価基準] 〈色素沈着〉 A:なくなった B:明らかに少なくなった C:いくらか少なくなった D:殆ど変化がなかった E:かえって多くなった 〈紅斑〉 A:対照例と差がない B:対照例と殆んど差がない C:対照例に比べて多少紅斑が目立つ D 対照例に比べて相当紅斑が目立つ E 対照例に比べて明らかに紅斑が目立つ[Evaluation Criteria] <Pigmentation> A: disappeared B: apparently decreased C: somewhat decreased D: hardly changed E: increased rather <erythema> A: difference from control example No: B: Almost no difference from control C: Erythema is slightly more noticeable than control D Dery erythema is more noticeable than control E E Clearly more erythema is noticeable than control
【0057】[結果]結果を表3に示す。[Results] The results are shown in Table 3.
【表3】 [Table 3]
【0058】表3に示す通り、本発明の米糠抽出物を配
合したクリームは、すぐれた色素沈着防止効果を具え、
しかも皮膚に対する刺激が少なく安全性が高い。As shown in Table 3, the cream containing the rice bran extract of the present invention has an excellent pigmentation preventing effect,
Moreover, there is little irritation to the skin and the safety is high.
【0059】[0059]
【発明の効果】本発明の酸性水性媒体による米糠抽出物
を配合してなる美白化粧料は、有効成分の上記抽出物の
示す強いチロシナーゼ活性抑制作用により、シミ、ソバ
カスなど皮膚への色素沈着を顕著に抑制或いは軽減する
と共に、該抽出物が天然物由来であるため皮膚に対する
刺激性が少なく安全性にもすぐれている。EFFECT OF THE INVENTION The whitening cosmetic composition of the present invention comprising the rice bran extract in an acidic aqueous medium is effective in inhibiting the tyrosinase activity of the above-mentioned extract of the active ingredient to prevent pigmentation on the skin such as spots and freckles. The extract is significantly suppressed or reduced, and since the extract is derived from natural products, it is less irritating to the skin and has excellent safety.
【図1】図1は、試験例1の各試料のドーパ値を示すグ
ラフである。FIG. 1 is a graph showing the Dopa value of each sample of Test Example 1.
【図2】図2は、試験例1の各試料のMTT値を示すグ
ラフである。FIG. 2 is a graph showing the MTT value of each sample of Test Example 1.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 大澤 豊 大阪市西区北堀江1丁目6番8号 株式会 社テクノーブル内 Fターム(参考) 4C083 AA112 AA122 AB032 AB052 AC022 AC102 AC122 AC242 AC302 AC422 AC482 AD092 AD112 AD272 AD282 AD332 AD572 CC04 CC05 CC07 CC23 DD27 EE10 EE16 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Yutaka Osawa 1-6-8 Kitahorie, Nishi-ku, Osaka-shi F-term in Technoble Co., Ltd. 4C083 AA112 AA122 AB032 AB052 AC022 AC102 AC122 AC242 AC302 AC422 AC482 AD092 AD112 AD272 AD282 AD332 AD572 CC04 CC05 CC07 CC23 DD27 EE10 EE16
Claims (4)
出物を配合したことを特徴とする美白化粧料。(1) A whitening cosmetic comprising an extract obtained by extracting rice bran with an acidic aqueous medium.
1に記載の美白化粧料。2. The whitening cosmetic according to claim 1, wherein the pH of the acidic aqueous medium is 5 or less.
2に記載の美白化粧料。3. The whitening cosmetic according to claim 2, wherein the pH of the acidic aqueous medium is from 1 to 4.
溶媒との混合物を用いる請求項1から3に記載の美白化
粧料4. The whitening cosmetic according to claim 1, wherein water or a mixture of water and a water-miscible organic solvent is used as the aqueous medium.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11272899A JP4424561B2 (en) | 1999-03-15 | 1999-03-15 | Whitening cosmetics |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11272899A JP4424561B2 (en) | 1999-03-15 | 1999-03-15 | Whitening cosmetics |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2000264834A true JP2000264834A (en) | 2000-09-26 |
JP2000264834A5 JP2000264834A5 (en) | 2005-01-06 |
JP4424561B2 JP4424561B2 (en) | 2010-03-03 |
Family
ID=14594064
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP11272899A Expired - Lifetime JP4424561B2 (en) | 1999-03-15 | 1999-03-15 | Whitening cosmetics |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002255784A (en) * | 2001-03-02 | 2002-09-11 | Oriza Yuka Kk | Composition for beautiful skin |
JP2005015450A (en) * | 2003-06-30 | 2005-01-20 | Kanebo Cosmetics Inc | Skin cosmetic |
JP2005132850A (en) * | 2001-01-31 | 2005-05-26 | Tekunooburu:Kk | Compounding agent for cosmetic and cosmetic compounded with the same |
JP2005255613A (en) * | 2004-03-11 | 2005-09-22 | Kyoei Kagaku Kogyo Kk | Cosmetic |
JP2006193487A (en) * | 2005-01-14 | 2006-07-27 | Kyoei Kagaku Kogyo Kk | Cosmetic |
JP2010273598A (en) * | 2009-05-28 | 2010-12-09 | Kanazawa Inst Of Technology | Method for manufacturing tyrosinase activity inhibitor |
JP2011036168A (en) * | 2009-08-10 | 2011-02-24 | Okuno Chemical Industries Co Ltd | Wheat flour and method for producing the same |
JP2016179970A (en) * | 2016-02-29 | 2016-10-13 | 株式会社創研 | Whitening agent and internal or external composition comprising the same |
-
1999
- 1999-03-15 JP JP11272899A patent/JP4424561B2/en not_active Expired - Lifetime
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005132850A (en) * | 2001-01-31 | 2005-05-26 | Tekunooburu:Kk | Compounding agent for cosmetic and cosmetic compounded with the same |
JP2002255784A (en) * | 2001-03-02 | 2002-09-11 | Oriza Yuka Kk | Composition for beautiful skin |
JP2005015450A (en) * | 2003-06-30 | 2005-01-20 | Kanebo Cosmetics Inc | Skin cosmetic |
JP2005255613A (en) * | 2004-03-11 | 2005-09-22 | Kyoei Kagaku Kogyo Kk | Cosmetic |
JP4573545B2 (en) * | 2004-03-11 | 2010-11-04 | 共栄化学工業株式会社 | Cosmetics |
JP2006193487A (en) * | 2005-01-14 | 2006-07-27 | Kyoei Kagaku Kogyo Kk | Cosmetic |
JP2010273598A (en) * | 2009-05-28 | 2010-12-09 | Kanazawa Inst Of Technology | Method for manufacturing tyrosinase activity inhibitor |
JP2011036168A (en) * | 2009-08-10 | 2011-02-24 | Okuno Chemical Industries Co Ltd | Wheat flour and method for producing the same |
JP2016179970A (en) * | 2016-02-29 | 2016-10-13 | 株式会社創研 | Whitening agent and internal or external composition comprising the same |
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