JP2000264834A - Bleaching cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject cosmetic such as a milky lotion, having strong tyrosinase activity inhibitory action, capable of inhibiting or reducing pigmentation to the skin such as fleckles, having slight irritancy to the skin, and also having high safety to the skin, by including an extract obtained by subjecting rice bran to extraction with an acidic aqueous midium. SOLUTION: This bleaching cosmetic is obtained by including an extract from rice bran subjected to extraction using an acidic aqueous medium adjusted to pH 1 to 4 by lactic acid or the like. The above-mentioned aqueous medium is desirable to be water or a mixture of water with a water-miscible organic solvent. The amount of the above-mentioned extract to be compounded is pref. 0.01 to 0.5 wt.% on a solid basis, when normal rice bran is used. The above- mentioned extract, when normal rice bran is to be used as an object for extraction, is obtained, for example, by the following process comprising first, if necessary, conducting enzyme deactivation treatment such as heating on rice bran, and then, adding pref. 2 to 5 wt. times of the above-mentioned acidic aqueous medium based on the weight of the dried rice bran and conducting an extraction normally at 4 to 40 deg.C for about 6 h to 7 days.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic which has an excellent whitening effect and is highly safe for the skin.

[0002]

2. Description of the Related Art Various whitening agents such as kojic acid, ascorbic acid derivatives and hydroquinone derivatives have been conventionally proposed for the purpose of preventing pigmentation caused by sunburn or aging, especially spots and freckles, and improving symptoms. Is marketed. However,
These conventional whitening agents, in addition to the whitening effect itself cannot always be sufficiently satisfactory, may cause skin irritation problems when the amount thereof is increased to maximize the whitening effect. There are many points that need to be improved in terms of practicality.

[0003]

DISCLOSURE OF THE INVENTION In view of the problems of the prior art, the present inventors have attempted to provide a whitening component in natural products in order to provide a whitening cosmetic which has an excellent whitening effect and is highly safe for the skin. As a result of intensive research on the search for and application of it to cosmetics, the specific extract fraction of rice bran has an excellent whitening effect based on the tyrosinase activity inhibitory effect, and also has skin irritation due to its natural origin. They have found that there are few, and have completed the present invention.

[0004]

That is, the present invention is a whitening cosmetic comprising an extract obtained by extracting rice bran with an acidic aqueous medium.

Hereinafter, the present invention will be described in detail. As the rice bran referred to in the present invention, besides the so-called rice bran obtained when milling brown rice, it is also possible to use brown rice itself, that is, if all or a part thereof is composed of a component equivalent to rice bran. Well, there is no particular limitation on the type.

[0006] As the acidic aqueous medium used for extraction of rice bran, generally, water adjusted to pH 5 or less, preferably 1 to 4 with acid is used. Instead of water, a mixture of water and a water-miscible organic solvent can be used.

The acids used for adjusting the pH of the aqueous medium include:
There are inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid, and organic acids such as acetic acid, lactic acid, malic acid and citric acid. Among them,
Particularly, use of lactic acid is preferred. When a water-miscible organic solvent is used in combination, examples of the solvent include monohydric alcohols such as methanol, ethanol, and propanol; and polyhydric alcohols such as ethylene glycol, propylene glycol, 1.3-butylene glycol, diethylene glycol, and glycerin. And esters such as ethyl acetate, butyl acetate and methyl propionate, and ketones such as acetone, methyl ethyl ketone and dioxane. The mixing ratio of these organic solvents to water is generally 70% by weight or less, preferably 50% by weight or less.

A method for preparing a rice bran extract using such an acidic aqueous medium, for example, in the case of using ordinary rice bran as an extract, is as follows. That is, first, rice bran is subjected to an enzyme inactivation treatment and a degreasing treatment by heating or the like, if necessary.
Add 10 to 10 times, preferably 2 to 5 times the amount of acidic aqueous medium, mix well, and extract with stirring or without stirring. The extraction conditions vary depending on the composition of the solvent used, pH, etc., but are generally at 4 to 40 ° C. for about 6 hours to 7 days.

After completion of the extraction, the solid is removed by performing a solid-liquid separation operation such as filtration and centrifugation, and the extract obtained here is generally further used in a whitening cosmetic. The pH is adjusted to the range of 6.0 to 8.0, which is the pH range obtained, and the precipitate is removed by filtration or the like to obtain a rice bran extract.

[0010] In the above-mentioned step, for example, the extract after removing solids may be subjected to an enzyme treatment with a protease or the like.
Thereby, the storage stability of the rice bran extract can be enhanced.

The rice bran extract thus obtained is used as it is, or further adjusted to an appropriate concentration by concentration under reduced pressure or the like, and blended into a whitening cosmetic, or in some cases, a conventional method such as a spray drying method or a freeze drying method. And then blended into the cosmetic.

The amount of the above-mentioned rice bran extract in the whitening cosmetic of the present invention varies depending on the rice bran raw material used and the like. However, when ordinary rice bran is used, it is generally 0.1% as a solid content. 002 to 2% by weight, preferably 0.01 to
It is in the range of 0.5% by weight. 0.002% by weight
Below, it becomes difficult to obtain the desired whitening effect,
On the other hand, if the amount is more than 2% by weight, it is difficult to expect a further improvement in the whitening effect in proportion to the amount of the compounding.

When an extract obtained by using brown rice or the like as a rice bran raw material is incorporated into a cosmetic, the amount is adjusted in consideration of the rice bran content in the raw material used or the whitening effect of the extract.

The whitening cosmetic composition of the present invention contains, in addition to the rice bran extract, which is an essential component, oil and fat components, surfactants, moisturizers, thickeners, preservatives and bactericides which are commonly used in cosmetics.
Powder components, ultraviolet absorbers, pigments, fragrances, and the like are appropriately compounded as necessary, and the components are used in the form of creams, emulsions, lotions, ointments, packs, and the like.

The fats and oils include, for example, oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, coconut oil, palm oil, cacao oil and mink oil: waxes such as beeswax, carnauba wax and lanolin: fluid Hydrocarbons such as paraffin, petrolatum, paraffin wax and squalane: fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid and isostearic acid: higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol: isopropyl myristate, Synthetic esters such as isopropyl palmitate and butyl oleate are exemplified.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene hydrogenated castor oil. , Non-ionic surfactants such as polyoxyethylene sorbitol fatty acid esters: fatty acid salts, alkyl sulfates, alkyl benzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates Salt, polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate, etc. On surfactant: quaternary ammonium salts, primary to tertiary fatty amine salts, trialkyl benzyl ammonium salts, alkyl pyridinium salts, 2-
Alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N.P. Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts: N-dimethyl-N-alkyl-N-
Carboxymethylammoniobetaine, N.P. N. Zwitterionic surfactants such as N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N'N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine, and the like. .

Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1.3-
There are mucoboli saccharides such as butylene glycol, polyethylene glycol, sorbitol, pyrrolidone carboxylate, and hyaluronic acid, and urea.

Examples of the thickener include tragacanth gum,
Examples include sodium alginate, pectin, sodium carboxymethylcellulose, polyvinyl alcohol, carboxyvinyl polymer, polyacrylic acid and its derivatives, gelatin, polyvinylpyrrolidone, veegum, methylpolysiloxane, and dextran.

Examples of the antiseptic disinfectant include urea, methyl parahydroxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate,
Phenoxyethanol, dichlorophen, hexachlorophen, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, ethanol and the like.

Examples of the powder component include titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, sericite, nylon powder, silk powder and the like. is there.

Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate, ethylhexyl paramethoxycinnamate, octyl cinnamate, 2.4-dihydroxybenzophenone, and 2-hydroxy-4-methoxybenzophenone-. 5-sulfonate, 2- (2-hydroxy-5-
Methylphenyl) benzotriazole, urocanic acid,
And ethyl urocanate.

The whitening cosmetic composition of the present invention may further contain other cosmetic active ingredients, including other whitening agents, and may be used in combination, as long as the effectiveness of the rice bran extract of the present invention is not impaired. Such substances include, for example, kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives (L
Whitening agents such as ascorbic acid-2-phosphate ester salt, L-ascorbic acid-2-glucoside), ellagic acid, resorcinol derivatives (such as 4-n-butylresorcinol), soybean extract, and saxifrage extract: vitamin E and Derivatives (vitamin E nicotinate, vitamin E linoleate, etc.), nicotinic acid and its derivatives (nicotinamide, benzyl nicotinate, etc.), α-hydroxy acids (lactic acid, citric acid, α-hydroxyoctanoic acid, etc.), diisopropylamine dichloro Acetate, γ
Anti-aging and skin roughness improving agents such as -amino-β-hydroxybutyric acid.

Hereinafter, the present invention will be described more specifically with reference to Production Examples, Examples, and Test Examples, but the present invention is not limited thereto. In the following,% and parts mean% by weight and parts by weight, respectively.

Production Example 1 Preparation of Rice Bran Extract Solution To 400 g of rice bran was added a 0.1 M lactic acid aqueous solution (pH 2.6) 12
After adding rice bran and an aqueous lactic acid solution with stirring, the mixture was allowed to stand at room temperature for 2 days. Next, the insoluble matter was removed by filtration, the pH of the filtrate was adjusted to 8.5 with a 1N aqueous sodium hydroxide solution, and the resulting insoluble matter was filtered off. The filtrate was adjusted to pH 6.5 with lactic acid to obtain a rice bran extract solution. (Yield: about 800 g, solids concentration: 4.4%).

Preparation Example 2 Preparation of Rice Bran Extract Solution As an extraction medium, 10%
A rice bran extract solution was obtained in the same manner as in Production Example 1 except that an ethanol-containing 0.1 M aqueous lactic acid solution (pH 2.7) was used (yield: about 750 g, solid content concentration: 5.2%).

Production Example 3 Preparation of Rice Bran Extract Solution As an extraction medium, 0.1 M lactic acid aqueous solution was used instead of 0.1 M lactic acid aqueous solution.
Production Example 1 except that an aqueous M phosphoric acid solution (pH 1.6) was used.
A rice bran extract solution was obtained in the same manner as described above (yield: about 700
g, solid content concentration: 4.4%)

Production Example 4 Preparation of Rice Bran Extract Solution As an extraction medium, 0.1 M lactic acid aqueous solution was used instead of 0.1 M lactic acid aqueous solution.
A rice bran extract solution was obtained in the same manner as in Production Example 1 except that N hydrochloric acid (pH 1.1) was used (yield: about 650 g, solid content concentration: 4.0%).

Production Example 5 Preparation of Rice Bran Extract Solution As an extraction medium, 0.1 M lactic acid aqueous solution was used instead of 0.1 M lactic acid aqueous solution.
Except for using M citric acid (pH 2.3), a rice bran extract solution was obtained in the same manner as in Production Example 1 (yield: about 600 g,
(Solid concentration: 4.8%)

Production Example 6 Preparation of Rice Bran Extract Solution A solution of 0.1 M lactic acid aqueous solution (pH 2.6) was added to 400 g of rice bran.
After adding rice bran and an aqueous lactic acid solution with stirring, the mixture was allowed to stand at room temperature for 2 days. Next, insoluble materials were removed by filtration, the pH of the filtrate was adjusted to 7.5, and the amount of
g, and the mixture was subjected to an enzyme treatment at 40 ° C. for 2 hours. The treated liquid was filtered, and the pH of the filtrate was adjusted to 8.5 with a 1N aqueous solution of sodium hydroxide.
The resulting insolubles were filtered off and the filtrate was adjusted to pH 6 with lactic acid.
-5 was obtained as a rice bran extract solution (yield: about 750 g,
Solids concentration: 4.7%).

Preparation Example 7 Preparation of Rice Bran Extract Powder 100 g of the rice bran extract obtained in Preparation Example 1 was concentrated under reduced pressure and freeze-dried to obtain about 4.4 g of rice bran extract powder.

Production Example 8 Preparation of Rice Bran Extract Solution A rice bran extract solution was obtained in the same manner as in Production Example 1 except that brown rice was used instead of rice bran (yield: about 1).
000 g, solid content concentration: 0.5%).

Example 1 Cream [Component A] Part Liquid paraffin 5.0 Hexalan (Glyceryl trioctanoate manufactured by Technoble Co., Ltd.) 4.0 Paraffin 5.0 Glyceryl monostearate 2.0 Polyoxyethylene (20) sorbitan Monostearate 6.0 Butyl paraben 0.1 [Component B] Rice bran extract solution obtained in Production Example 1 5.0 Glycerin 5.0 Sodium carboxymethyl cellulose 0.1 Methyl paraben 0.1 Moiston C (Co., Ltd.) NMF component manufactured by Technoble) 1.0 Purified water Amount to make the total amount 100 parts [Component C] Fragrance Appropriate amount After heating each of the above components A and B to 80 ° C. or higher, they were mixed by stirring. After cooling to 50 ° C., the C component was added and further stirred and mixed to prepare a cream.

Example 2 Emulsion [Component A] Part Liquid paraffin 6.0 Hexalan (Glyceryl trioctanoate manufactured by Technoble Co., Ltd.) 4.0 Jojoba oil 1.0 Polyoxyethylene (20) sorbitan monostearate 2.0 Soybean lecithin 1.5 Methylparaben 0.15 Ethylparaben 0.03 [Component B] Rice bran extract solution obtained in Production Example 2 5.0 Glycerin 3.0 1.3 Butylene glycol 2.0 Sodium carboxymethylcellulose 0. 3 Sodium hyaluronate 0.01 Purified water Amount to make the total amount 100 parts [Component C] Perfume Appropriate amount After heating each of the above components A and B to 80 ° C. or higher, they were mixed by stirring. After cooling this to 50 ° C., the C component was added and further stirred and mixed to prepare an emulsion.

Example 3 Lotion [Component] Part Rice bran extract solution obtained in Production Example 1 5.0 Ethanol 10.0 Glycerin 3.0 1.3 Butylene glycol 2.0 Methylparaben 0.2 Citric acid 1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Perfume Appropriate amount Purified water An amount that gives a total amount of 100 parts The above components were mixed to prepare a lotion.

Example 4 Pack [Components] Part Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.2 Rice bran extract solution obtained in Production Example 2 3.0 Fragrance 1 0.0 Purified water Appropriate amount An amount that makes the total amount 100 parts. The above components were stirred and mixed to prepare a pack.

Example 5 Facial Cleanser [Component] Part Stearic Acid 15.0 Lauric Acid 5.0 Myristate 15.0 Glyceryl Monostearate 4.0 Potassium Hydroxide 7.0 Glycerin 8.0 Obtained in Production Example 7 Rice bran extract powder 0.2 Methyl paraben 0.2 Purified water Amount to make the total amount 100 parts The above components were heated to 85 ° C and mixed by stirring to prepare a face wash.

Example 6 Cream In Example 1, except that the rice bran extract solution of Production Example 4 was used instead of the rice bran extract solution of Production Example 1,
A cream was prepared in the same manner as described above.

Example 7 Emulsion Example 1 was repeated except that the rice bran extract solution of Production Example 5 was used in place of the rice bran extract solution of Production Example 2.
An emulsion was prepared in the same manner as described above.

Example 8 Cream In Example 1, except that the rice bran extract solution of Production Example 7 was used instead of the rice bran extract solution of Production Example 1,
A cream was prepared in the same manner as described above.

Example 9 Cream The procedure of Example 1 was repeated, except that 5.0 parts of the rice bran extract solution of Preparation Example 1 was replaced with 4.0 parts of the extract solution and 1.0 part of kojic acid. A cream was prepared as in Example 1.

Comparative Example Cream A cream was prepared in the same manner as in Example 1, except that 1.0 part of kojic acid was used instead of 5.0 parts of the rice bran extract solution of Production Example 1.

Comparative Example Cream A cream was prepared in the same manner as in Example 1, except that the rice bran extract solution of Production Example 1 was not used.

Test Example 1 Inhibition of Intracellular Tyrosinase Activity [Test Method] Cultured B16 mouse melanoma cells were
Seed 8 × 10 ³ / well in a well microplate, 10%
Eagle minimum essential medium containing calf serum (FBS) (ME
M), the cells were pre-cultured for 1 day at 37 ° C. and 5% CO ₂ , and the culture solution was added to the rice bran extract solution of Production Example 1 in 1.
1 added to a concentration of 0, 2.5 or 5.0%
Eagle MEM containing 0% FBS, or Production Examples 2 to 5
Was replaced with 10% FBS-containing Eagle MEM added to a concentration of 5.0%, respectively, and cultured under the same conditions for 4 days. Next, the culture solution was removed, 5 mM L-dopa or 0.2% MTT was added to the cell treatment solution to which a surfactant (Triton X-100) was added, and a tyrosinase reaction was performed at 37 ° C. Then, a microplate reader (Mode 1450, manufactured by Bio-Rad)
And the dopa value at a wavelength of 490 nm is also 570-630.
MTT values were measured in nm. For comparison,
The same test was performed when 2 mM kojic acid was added instead of the rice bran extract solution of Production Example 1 and when no sample was added (blank).

[Results] The dopa value of each sample is shown in FIG. 1, and the MTT value of each sample is shown in FIG. From the results shown in FIGS. 1 and 2, it can be seen that the rice bran extract of the present invention significantly inhibits intracellular tyrosinase activity in a concentration-dependent manner without reducing cell activity.

Test Example 2 Pigmentation Inhibition Test The in vivo whitening action of the rice bran extract of the present invention was examined by a pigmentation test using guinea pigs.

[Sample] The rice bran extract of Production Example 1 was diluted with purified water to a solid content of 0.2% and used as a sample. [Test Method] The hair of a colored guinea pig (male, 8 weeks of age) at the center of the back at 60 mm in the vertical direction and 30 mm in the horizontal direction was shaved, and this part was divided into two right and left parts. A sample solution was applied to one of the compartments and purified water as a control was applied to the other side twice each in the morning and evening for 5 days each for 5 ml for 6 days, and once every day immediately before application in the morning at 500 mJ / cm ² to the application site. -B was irradiated, and in the evening of the sixth day, the pigmentation state of the irradiated site was visually observed and evaluated according to the following criteria.

[0047]

[Results] The results are shown in Table 1.

[Table 1]

As shown in Table 1, the rice bran extract of the present invention has an effect of remarkably preventing pigmentation on skin due to ultraviolet rays.

Test Example 3 Skin Irritation Evaluation Test The skin irritation of various whitening agents was evaluated by a neutral red uptake test using cultured rabbit corneal cells (SIRC), which is an alternative to the ocular mucosal irritation test.

[Samples] (1) Rice bran extract solution of Production Example 1 (2) Kojic acid (3) Arbutin

[Test Method] After 5,000 SIRCs were seeded on a 96-well microplate and cultured in Eagle's minimum essential medium (MEM) containing 5% calf serum for 3 days, a sample was added to the solid content concentration of 0.2%. And cultured for 2 days. Next, the SRIC was cultured in an Eagle MEM medium containing 0.05% neutral red (NR) for 3 hours, and the NR uptake rate was determined for the obtained SRIC, and the skin irritation of each sample was evaluated.

[Results] The results are shown in Table 2.

[Table 2]

As shown in Table 2, the rice bran extract of the present invention
There is less irritation to the skin as compared to existing whitening agents, which makes it possible to obtain safer whitening cosmetics.

Test Example 4 Monitor Test The creams of Example 1, Comparative Examples and Control Examples were examined for their inhibitory effect on skin pigmentation and skin irritation. [Test method] Twenty women aged 18 to 50 years randomly selected as subjects were given each cream twice a day (morning,
Evening) The composition was applied to the upper back for one month, and the state of pigmentation of the applied part and erythema of the skin were visually observed, and evaluated based on the following evaluation criteria.

[Evaluation Criteria] <Pigmentation> A: disappeared B: apparently decreased C: somewhat decreased D: hardly changed E: increased rather <erythema> A: difference from control example No: B: Almost no difference from control C: Erythema is slightly more noticeable than control D Dery erythema is more noticeable than control E E Clearly more erythema is noticeable than control

[Results] The results are shown in Table 3.

[Table 3]

As shown in Table 3, the cream containing the rice bran extract of the present invention has an excellent pigmentation preventing effect,
Moreover, there is little irritation to the skin and the safety is high.

[0059]

EFFECT OF THE INVENTION The whitening cosmetic composition of the present invention comprising the rice bran extract in an acidic aqueous medium is effective in inhibiting the tyrosinase activity of the above-mentioned extract of the active ingredient to prevent pigmentation on the skin such as spots and freckles. The extract is significantly suppressed or reduced, and since the extract is derived from natural products, it is less irritating to the skin and has excellent safety.

[Brief description of the drawings]

FIG. 1 is a graph showing the Dopa value of each sample of Test Example 1.

FIG. 2 is a graph showing the MTT value of each sample of Test Example 1.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Yutaka Osawa 1-6-8 Kitahorie, Nishi-ku, Osaka-shi F-term in Technoble Co., Ltd. 4C083 AA112 AA122 AB032 AB052 AC022 AC102 AC122 AC242 AC302 AC422 AC482 AD092 AD112 AD272 AD282 AD332 AD572 CC04 CC05 CC07 CC23 DD27 EE10 EE16

Claims (4)

[Claims] (1) A whitening cosmetic comprising an extract obtained by extracting rice bran with an acidic aqueous medium. 2. The whitening cosmetic according to claim 1, wherein the pH of the acidic aqueous medium is 5 or less. 3. The whitening cosmetic according to claim 2, wherein the pH of the acidic aqueous medium is from 1 to 4. 4. The whitening cosmetic according to claim 1, wherein water or a mixture of water and a water-miscible organic solvent is used as the aqueous medium.