JPH11263720A - Antiaging agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antiaging agent for skin capable of retaining the tensity and elasticity of the skin and maintaining a youthful state of the skin by including a plant extract capable of manifesting inhibiting actions on an elastase and glutathione therein. SOLUTION: This antiaging agent is obtained by including a plant extract capable of manifesting inhibiting actions on an elastase (preferably one or more kinds of extracts selected from Sophora flavescens Aiton, Uncaria gambir Roxb., Syzygium aromaticum Merrill et Perry, Saxifraga stolonifera Meerburg, Humulus lupulus Linne, Rosmarinus officinalis Linne, Lithospermum erythrorhizon Sielold et Zuccarini, Hypericum Perforatum Linne, Hypericum erectum Thunberg and Thea sinensis O. Kuntze) and glutathione. The amount of the formulated plant extracts based on the antiaging agent is preferably 0.001-5.0 wt.% and the amount of the formulated glutathione based on the antiaging agent is preferably 0.0005-10.0 wt.%. The antiaging agent can be formulated with a beautifying and whitening agent, a humectant, an antioxidant, etc. Furthermore, the antiaging agent can preferably be applied to a cosmetic and a dosage form such as an aqueous solution system, a solubilized system or an emulsion system can be assumed.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an anti-aging agent.
More specifically, the present invention relates to a skin anti-aging agent that has an elastase inhibitory action, maintains skin firmness and elasticity, and can maintain a youthful skin condition.

[0002]

2. Description of the Related Art Conventionally, the necessity of an anti-aging agent has been considered, but the mechanism and definition of aging have not been clarified. Measurement and visual observation of skin color for judgment.

However, in recent years, research on aging has been promoted, and aging is an important factor as a cause of skin aging from a macro perspective, and the effects of drying, oxidation, sunlight (ultraviolet rays) and the like are also important. Has been cited as a direct factor related to

[0004]

As specific phenomena of skin aging, reduction of collagen and elastin in skin dermis, reduction of mucopolysaccharides such as hyaluronic acid, damage of cells by ultraviolet rays, etc. are known. I have.

[0005] Of these, elastin crosslinks each other and contributes to the elasticity of the tissue. However, elastin, which is an elastin-disrupting enzyme, is overexpressed by exposure to ultraviolet light and aging, and elastin is denatured. It is believed that destruction leads to reduced elasticity of the skin.

[0006] Therefore, it is important to prevent aging of the skin by suppressing the action of elastase and preventing the degeneration and destruction of elastin which gives the skin elasticity and firmness.

[0007] Accordingly, an anti-aging agent is provided which has an effect of preventing skin aging and maintaining a youthful skin state by suppressing and maintaining elastase activity to restore and maintain skin firmness and elasticity. A patent for this purpose has been filed (Japanese Patent Application No. 8-188234).

However, its effect is not always sufficient, and there has been a demand for the development of a drug having a strong anti-aging effect in order to efficiently prevent skin aging.

In view of the above problems, the present inventors have studied a wide variety of substances on the action of elastase inhibitory activator to increase the anti-aging effect. As a result, surprisingly, glutathione having an antioxidant action was converted to elastase inhibitor. It has been found that when combined with an activator, the anti-aging effect of the elastase inhibitory activator is significantly enhanced, and the present invention has been completed.

[0010]

That is, the present invention provides an anti-aging agent comprising a plant extract having elastase inhibitory activity and glutathione.

[0011] Further, the present invention provides a method for producing a plant, comprising the steps of:
(Renoki), Clove, Saxifraga, Hops, Rosemary, Sikon, Hypericum, Hypericum perforatum, Chaex, or an extract of two or more selected from the foregoing anti-aging agents, Is provided.

Further, the present invention provides a skin external preparation characterized by containing the above-mentioned anti-aging agent.

[0013]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The configuration of the present invention will be described below in detail.

The plant extract used in the present invention may be any plant extract as long as it exhibits an elastase inhibitory action.
Clara (scientific name: Sophora flavescens Aiton), Asenyak (scientific name: Uncaria gambir Roxb.), Chow (scientific name:
Syzygium aromaticum Merrill et Perry), Saxifraga (scientific name: Saxifraga stolonifera Meerburg), Hop (scientific name: Humulus lupulus Linne), Rosmari (scientific name: Rosmarinus officinalis Linne), Sikon (scientific name:
Lithospermum erythrorhizon Sielold et Zuccarin
i), Hypericum (scientific name: Hypericum Perforatum Linn)
e), Hypericum perforatum (scientific name: Hypericum erectum)
Thunberg), Chaex (scientific name: Thea sinensis O.Kun)
One or more extracts selected from tze) are preferred.

[0015] The plant extract is an organic solvent extract of a plant. Specifically, for example, dry Clara powder or cut non-dried Clara is cut into methanol, ethanol, 1,3-
Extract with stirring at an elevated temperature of 30 to 70 ° C for 1 to 10 hours with an alcohol such as butylene glycol, or extract at room temperature for 1 to 20 days, filter, concentrate the filtrate, and concentrate under reduced pressure to dryness. Can be used. In the present invention, the plant extract can be used either in the above-mentioned concentrated liquid stage or in the dried product stage.

The amount of the plant extract in the present invention is not particularly limited, but is generally 0.0005 to 10% by weight, preferably 0.001 to 10% by weight, based on the total amount of the anti-aging agent or the external preparation for skin. It is 5.0 weight. The amount is 0.0
If the amount is less than 005% by weight, the elastase inhibitory action tends to be poor. Conversely, if the amount exceeds 10% by weight, an increase in the effect cannot be substantially expected and formulation becomes difficult, which is not preferable. Further, even if the content exceeds 5.0% by weight, the effect is not so greatly improved.

Glutathione used in the present invention is an SH compound which is most present in a living body, has a function of reacting enzymatically and non-enzymatically with proteins and other disulfides, and has a function of maintaining the SH. Converted to oxidized glutathione.

The amount of glutathione in the present invention is as follows:
Although not particularly limited, it is generally 0.0001 to 20% by weight, preferably 0.0005 to 10.0% by weight, based on the total amount of the anti-aging agent or the external preparation for skin. The amount is
If the amount is less than 0.0001% by weight, the anti-aging effect (wrinkles / small wrinkles, firmness / tarumi) tends to be poor.
If the amount is more than 0% by weight, an increase in the effect cannot be substantially expected, and it is not preferable because formulation becomes difficult. Also, 1
Even if it is added in an amount exceeding 0.0% by weight, no significant improvement in the effect is observed.

The anti-aging agent of the present invention may contain, in addition to the above essential components, components usually used in external preparations for skin such as cosmetics and pharmaceuticals, for example, whitening agents, moisturizing agents, antioxidants, oily components, and ultraviolet absorbers. , An anti-inflammatory agent, a surfactant, a thickener, an alcohol, a powder component, a coloring material, an aqueous component, water, various skin nutrients, and the like can be appropriately compounded as necessary.

The anti-aging agent of the present invention can be widely applied to cosmetics applied to the outer skin, skin external preparations such as quasi-drugs, particularly preferably cosmetics. A wide range of dosage forms such as an aqueous solution type, a solubilizing type, an emulsifying type, a powder type, an oil-liquid type, a gel type, an ointment type, an aerosol type, a water-oil two-layer system, a water-oil-powder three-layer system can be adopted. That is, basic cosmetics can be widely applied to various forms such as facial cleanser, lotion, milky lotion, cream, gel, essence (cosmetic), pack, mask and the like. In addition, makeup cosmetics can be widely applied to foundations and the like, and toiletry products can be widely applied to forms such as body soaps and soaps. Furthermore, quasi-drugs can be widely applied to various ointments and the like. The anti-aging agent of the present invention is not limited to these dosage forms and forms.

[0021]

Next, the present invention will be described in more detail by way of examples. Note that the present invention is not limited by this. The compounding amount is% by weight.

"Plant Extract" The plant extract used in the following examples is Clara root, Asenyaku (Gambir-no-ki).
Are leaves and shoots, butterflies are buds, and saxifrage is whole grass,
Hops are female ears, Rosmari is leaves and flowers of Mannenwa, Chikon is the root of purple, Hypericum perforatum and St. John's wort are the above-ground parts, Chaex is the leaves of tea tree,
Water was added to each of the cut pieces, and the mixture was heated to 50 ° C., further added with ethanol, extracted for 5 hours, filtered, and a concentrate obtained by distilling off the solvent of the filtrate was used.

Example 1 Cream (Formulation) Stearic acid 5.0% by weight Stearyl alcohol 4.0 Isopropyl myristate 18.0 Glycerin monostearate 3.0 Propylene glycol 10.0 Glutathione 2.0 Clara extract 0.01 Caustic potash 0.2 Preservatives Appropriate amount Fragrance Appropriate amount Ion-exchanged water Residue (Preparation method) Add propylene glycol, Clara extract, glutathione and caustic potash to ion-exchanged water, dissolve and heat to 70 ° C (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is gradually added to the water phase, and after the addition is completed, the temperature is maintained for a while to cause a reaction. Thereafter, the mixture is uniformly emulsified with a homomixer and cooled to 30 ° C. while stirring well.

Example 2 Cream Stearic Acid 2.0% by Weight Stearyl Alcohol 7.0 Hydrogenated Lanolin 2.0 Squalane 5.0 2-Octyldodecyl Alcohol 6.0 Polyoxyethylene (25 mol) Cetyl Alcohol Ether 3 0.0 Glycerin monostearate 2.0 Propylene glycol 5.0 Glutathione 0.1 Clove extract 0.05 Clara extract 0.1 Ethylparaben 0.3 Perfume Appropriate amount Ion exchange water Residue (Production method) Ion exchange water Add propylene glycol to
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the water phase, pre-emulsified, homogenized with a homomixer, and cooled to 30 ° C. with good stirring.

Example 3 Cream (Formulation) Solid paraffin 5.0% by weight Beeswax 10.0 Vaseline 15.0 Liquid paraffin 41.0 Glycerin monostearate 2.0 Polyoxyethylene (20 mol) sorbitan monolaurate Ester 2.0 Soap powder 0.1 Borax 0.2 Glutathione 1.5 Asenyaku extract 0.1 Ascorbic acid 2.0 Ethyl paraben 0.3 Perfume Appropriate amount Ion exchange water Residue (Production method) Soap powder and borax in ion exchange water Is added, and the mixture is heated and maintained at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is gradually added to the aqueous phase while stirring to carry out the reaction. After completion of the reaction, the mixture is uniformly emulsified with a homomixer, and cooled to 30 ° C. while stirring well after emulsification.

Example 4 Emulsion (Formulation) Stearic acid 2.5% by weight Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 10.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3.0 Triethanolamine 1.0 Carboxyvinyl polymer 0.05 (Product name: Carbopol 941, BFGoodrich Chemical company) Glutathione 0.5 Clara extract 0.01 Saxifrage extract 0.1 Ethylparaben 0.3 Perfume Appropriate amount Ion-exchanged water residue (Preparation method) Dissolve the carboxyvinyl polymer in a small amount of ion-exchanged water (phase A). Polyethylene glycol 1500 and triethanolamine are added to the remaining ion-exchanged water, dissolved by heating, and kept at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the water phase, pre-emulsification is performed, the phase A is added, and the mixture is uniformly emulsified with a homomixer.

Example 5 Emulsion (Formulation) Microcrystalline wax 1.0% by weight Beeswax 2.0 Lanolin 20.0 Liquid paraffin 10.0 Octyl paradimethylaminobenzoate 3.0 2-Ethylhexyl paramethoxycinnamic acid 4.0 Squalane 5.0 Sorbitan sesquioleate 4.0 Polyoxyethylene (20 mol) sorbitan monooleate 1.0 Propylene glycol 7.0 Glutathione 0.01 Hop extract 10.0 Clara extract 1. 0 Magnesium ascorbate phosphate 3.0 Silicon extract 1.0 Ethyl paraben 0.3 Perfume Appropriate amount Ion-exchange water residue (Production method) Add propylene glycol to ion-exchange water,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). While stirring the oil phase, the aqueous phase is gradually added thereto, and the mixture is uniformly emulsified with a homomixer. After emulsification, cool to 30 ° C with good stirring.

Example 6 Jelly (Formulation) 95% ethyl alcohol 10.0% by weight dipropylene glycol 15.0 polyoxyethylene (50 mol) oleyl alcohol ether 2.0 carboxyvinyl polymer 1.0 (trade name: Carbopol 940, BFGoodrich Chemical company) Caustic soda 0.15 L-arginine 0.1 Glutathione 0.5 Arbutin 3.0 Rosmari extract 7.0 Clara extract 1.0 Sodium bisulfite 0.01 2-hydroxy 4-Methoxybenzophenone sodium sulfonate 0.05 Ethylenediaminetetraacetate / trisodium / 2 water 0.05 methylparaben 0.2 Perfume Appropriate amount Ion-exchange water residue (Preparation method) Carbopol 940 is uniformly dissolved in ion-exchange water, while Rosmari in 95% ethanol Distillate,
Clara extract, glutathione, polyoxyethylene (5
0 mol) Oleyl alcohol ether is dissolved and added to the aqueous phase. Next, after adding other components, the mixture is neutralized with caustic soda and L-arginine to increase the viscosity.

Example 7 Beauty Essence (Prescription) (Phase A) Ethyl alcohol (95%) 10.0% by weight Polyoxyethylene (20 mol) octyldodecanol 1.0 Pantothenyl ethyl ether 0.1 Asenyak extraction Product 1.5 Clara extract 1.0 Glutathione 1.5 Methyl paraben 0.15 (B phase) Potassium hydroxide 0.1 (C phase) Glycerin 5.0 Dipropylene glycol 10.0 Carboxyvinyl polymer 0.2 (Product Name: Carbopol 940, BFGoodrich Chemical company) Purified water residue (Preparation method) Dissolve A phase and C phase uniformly, and add A to C phase
Add phases and solubilize. Next, after adding the phase B, the container is filled.

Example 8 Pack (Formulation) (A phase) Dipropylene glycol 5.0% by weight Polyoxyethylene (60 mol) hydrogenated castor oil 5.0 (B phase) Clara extract 0.01 Hypericum extract 0.1 Glutathione 2.0 Olive oil 5.0 Tocopherol acetate 0.2 Ethylparaben 0.2 Fragrance 0.2 (C phase) Polyvinyl alcohol 13.0 (Saponification degree 90, polymerization degree 2,000) Ethanol 7.0 Purified water residue (Production method) A phase, B phase, and C phase are each dissolved uniformly,
Add phase B to phase and solubilize. Next, this is added to the phase C, and then the container is filled.

Example 9 Solid Foundation (Formulation) Talc 43.1% by weight Kaolin 15.0 Sericite 10.0 Zinc white 7.0 Titanium dioxide 3.8 Yellow iron oxide 2.9 Black iron oxide 0.2 Squalane 8.0 Isostearic acid 4.0 POE sorbitan monooleate 3.0 Isocetyl octanoate 2.0 Clara extract 1.0 Sicon extract 0.5 Glutathione 0.5 Preservatives Appropriate amount Perfume Appropriate amount (Production method) talc-black The powdery components of iron oxide are thoroughly mixed in a blender, and the oily components of squalane-isocetyl octanoate, Clara extract, sicon extract, glutathione, preservatives, flavors are added, and the mixture is kneaded well.
Mold.

Example 10 Emulsion Type Foundation (Cream Type) (Prescription) (Powder) Titanium dioxide 5.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Zinc white 0.5 Bengala 0.3 Black iron oxide 0.2 (oil phase) Decamethylcyclopentasiloxane 0.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (aqueous phase) Purified water 50.0 1,3- Butylene glycol 4.5 Glutathione 10.0 Clara extract 1.5 Glucoside ascorbic acid 1.0 Sorbitan sesquioleate 3.0 Preservatives Appropriate amount Perfume Appropriate amount The powder part is added and homomixer-treated. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

Example 11 Emulsion Type Foundation (Cream Type) (Prescription) (Powder) Titanium dioxide 5.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Zinc white 0.5 Bengala 0.3 Black iron oxide 0.2 (oil phase) Decamethylcyclopentasiloxane 0.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (aqueous phase) Purified water 50.0 1,3- Butylene glycol 4.5 Glutathione 10.0 Clara extract 1.5 Asenyaku extract 1.5 Ascorbic acid glucoside 1.0 Sorbitan sesquioleate 3.0 Preservatives Appropriate perfume Appropriate (production method) After heating and stirring the aqueous phase Then, a powder portion sufficiently mixed and pulverized is added and subjected to a homomixer treatment. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

Example 12 Emulsified Foundation (Cream Type) (Prescription) (Powder) Titanium dioxide 5.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Zinc white 0.5 Bengala 0.3 Black iron oxide 0.2 (oil phase) Decamethylcyclopentasiloxane 0.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (aqueous phase) Purified water 50.0 1,3- Butylene glycol 4.5 Glutathione 10.0 Clara extract 1.5 Clove extract 1.5 Ascorbic acid glucoside 1.0 Sorbitan sesquioleate 3.0 Preservatives Appropriate perfume Appropriate (production method) After heating and stirring the aqueous phase Then, a powder portion sufficiently mixed and pulverized is added and subjected to a homomixer treatment. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

Comparative Example 1 Emulsion type foundation (cream type) Glutathione was removed from the formulation of Example 10 to prepare Comparative Example 1. (Prescription) (Powder part) Titanium dioxide 5.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Zinc white 0.5 Bengala 0.3 Black iron oxide 0.2 (Oil phase) Deca Methylcyclopentasiloxane 0.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (aqueous phase) Purified water 60.0 1,3-butylene glycol 4.5 Clara extract 1.5 Ascorbic acid glucoside 1.0 Sorbitan sesquioleate 3.0 Preservatives Appropriate amount Flavors Appropriate amount (Preparation method) After heating and stirring the aqueous phase, a well-mixed and pulverized powder portion is added, and a homomixer treatment is performed. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

Comparative Example 2 Emulsion type foundation (cream type) A comparative example 2 was prepared by removing the Clara extract from the formulation of Example 10. (Prescription) (Powder part) Titanium dioxide 5.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Zinc white 0.5 Bengala 0.3 Black iron oxide 0.2 (Oil phase) Deca Methylcyclopentasiloxane 0.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (aqueous phase) Purified water 60.0 1,3-butylene glycol 4.5 Ascorbic acid glucoside 1.0 Glutathione 1. 0 Sorbitan sesquioleate 3.0 Preservatives Appropriate amount Flavors Appropriate amount (Preparation method) After heating and stirring the aqueous phase, a well-mixed and pulverized powder portion is added, followed by homomixer treatment. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

[Monitoring test] 100 healthy adult women aged 25 to 57 years were randomly selected as subjects.
After using the emulsified foundations of Examples 10 to 12 and Comparative Examples 1 and 2 on the skin of the face for one day every day, the following criteria were used to improve the effect on wrinkles and fine wrinkles, and the effect on improving the skin firmness and skin roughness. Examined. The results are shown in "Table 1".

(A) Improvement effect on wrinkles and fine wrinkles Skin condition was visually observed and evaluated based on the following evaluation criteria. (Evaluation criteria) A: Disappeared cleanly. B: Slightly less noticeable. C: No change. D: Increased a little. E: Increased.

(B) Improving effect on skin firmness / tarmi The condition of the skin was visually observed and evaluated based on the following evaluation criteria. (Evaluation criteria) A: Very improved. B: Improved. C: No change. D: It became somewhat noticeable. E: It became noticeable.

In the above monitor test, when the emulsified foundations obtained in Examples 10 to 12 and Comparative Examples 1 and 2 were used, one subject had abnormal skin.
There was no name. In Example 10, similar results were obtained also in the emulsified foundation in which the Clara extract was replaced with the Acacia catechu extract.

[0041]

[Table 1]

As is clear from the results shown in Table 1, when the emulsion type foundations obtained in Examples 10 to 12 were used, the emulsion type foundations obtained in Comparative Examples 1 and 2 were used. It is recognized that wrinkles, fine wrinkles, and firmness and skin roughness of the skin have been improved as compared with the case where they were present. This indicates that the combination of an elastase inhibitor and glutathione is extremely effective for anti-aging effects such as wrinkles and fine wrinkles and skin firmness and tarmi.

[0043]

According to the present invention, an anti-aging agent exhibiting an excellent anti-aging effect can be provided by combining and combining an elastase inhibitor and glutathione.
That is, by combining and combining elastase inhibitor and glutathione, it is possible to suppress the denaturation of elastin, which is an elastic fiber, to maintain elastic, wrinkled and sagging skin, and to prevent skin aging. And an anti-aging agent having an excellent cosmetic effect such as maintaining a youthful skin condition.

Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI // A61K 7/035 A61K 7/035 (72) Inventor Kenshi 1050 Nippa-cho, Kohoku-ku, Yokohama-shi, Kanagawa Shiseido Daiichi Research Center Co., Ltd. (72) Inventor Masako Naganuma 1050 Nippa-cho, Kohoku-ku, Yokohama City, Kanagawa Prefecture Inside Shiseido Daiichi Research Center Co., Ltd. (72) Inventor Eiichiro Yagi 1050 Nippacho, Kohoku-ku, Yokohama City, Kanagawa Prefecture Shiseido Daiichi Co., Ltd. Inside Research Center (72) Inventor Ichiro Iwai 1050 Nippa-cho, Kohoku-ku, Yokohama City, Kanagawa Prefecture Inside Shiseido Daiichi Research Center Co., Ltd.

Claims (3)

[Claims] 1. An anti-aging agent comprising a plant extract having an elastase inhibitory effect and glutathione. 2. The plant extract exhibiting an elastase inhibitory action is selected from Clara, Acacia catechu (Gambi-Renoki), Clove, Saxifraga, Hops, Rosmari, Sikon, Hypericum, Hypericum perforatum, and Chaex. The anti-aging agent according to claim 1, wherein the anti-aging agent is one or two or more extracts. 3. An external preparation for skin, comprising the anti-aging agent according to claim 1 or 2.