JP2002097149A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin care preparation having synergistically improved preventing and ameliorating effects on dry skin and aging of the skin. SOLUTION: This skin care preparation comprises one or more kinds selected from an extract and extracted fractions of Puerariae Radix and/or one or more kinds selected from an extract and extracted fractions of Sanguisorba officinalis L. and one or more kinds selected from an extract and extracted fractions of malt. 4',7-Dihydroxyisoflavone may be used instead of the extract or the extracted fraction of Puerariae Radix.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin which has a synergistically improved effect of preventing and improving skin roughness and skin aging. More specifically, one or two or more selected from cuckoo extracts and extract fractions, and / or one or two or more selected from crimson extract and extract fractions, and malt extract And an external preparation for skin comprising a combination of one or more selected from extracted fractions.

[0002]

2. Description of the Related Art Skin inflammatory reactions and aging of the skin may progress due to aging, exposure to ultraviolet rays, oxidative stress due to reactive oxygen species generated in skin tissues, contact with drugs and various allergens, and the like. Are known. In the field of skin external preparations, many physiologically active ingredients such as active oxygen species scavengers, anti-inflammatory agents and anti-allergic agents have been searched and examined in order to prevent such skin inflammation and aging.
In recent years, reflecting the natural and plant orientation of consumers,
There is an increasing tendency to seek such components from plants.

[0003] However, among the above-mentioned components of plant origin which have already been reported, their activity is low, so that a considerably high concentration is required to obtain a sufficient effect when incorporated into a skin external preparation, There is a problem in terms of stability and safety, and there is something that gives an undesired color or odor to the external preparation for skin. At present, there are few things that can be satisfied in all aspects. Further, the skin inflammatory reaction, aging, and the like proceed with various factors involved in a complicated manner, and thus, even if a substance that acts on only a part of the reaction is not sufficiently effective.

[0004]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide an external preparation for skin which suppresses complex skin inflammatory reactions and the like, and has a synergistically improved effect of preventing and improving skin roughness and skin aging. The purpose was.

[0005]

It made various studies to solve the above problems [SUMMARY OF INVENTION The present inventors have Kakkonekisu (Puerariae
Radix ), one or more selected from extracts and extractable fractions, and / or Warmomko ( Sanguisorba offici)
nalis L.) extract and / or two or more selected from malt extracts and extract fractions in addition to one or more selected from extracts and extract fractions The present invention has been found to provide a good anti-inflammatory action and the like, and to provide a synergistic improvement in the effects of preventing and improving skin roughness and aging of the skin, thereby completing the present invention.

[0006] With respect to the cuckoo extract and the extract fraction, particularly those having a high content of 4 ', 7-dihydroxyisoflavone (daidzein) can provide a good effect, and 4', 7-dihydroxyisoflavone itself can be obtained. May be used.

[0007] In addition, the extract of cucumbers, mucopolysaccharide fragmentation suppression, active oxygen scavenging, antioxidant activity (Japanese Unexamined Patent Publication No.
24937), a fat synthesis promoting action (JP-A-11-199)
499), dermal papilla activating action (JP-A-11-24082)
No. 3) has been disclosed, and the action of promoting hyaluronic acid production has also been reported (Abstracts of the 120th Annual Meeting of the Pharmaceutical Society of Japan, Vol. 2, No. 58).
Page, 2000). Furthermore, a metabolite of 7-isopropoxy isoflavone has been reported to promote the synthesis of type I collagen (Calcified Tissue International
55 (5) 356-362 (1994)) shows that 4 ', 7-
It is well known that dihydroxyisoflavone has an antiacetylcholine effect, but no report has been made on its effect on collagen production or the like.

[0008] Further, with respect to the extract of Warmoko, the water extract of the dry powder has been disclosed to have a collagenase inhibitory effect (Japanese Patent Application Laid-Open No. 7-196526), and other hydroxyl radical or singlet oxygen scavenging effects (Japanese Patent Application Laid-Open No. Hei 7-13321).
6), the action of inhibiting the release of chemical mediators
0-36276), improvement of skin strength, keratinocyte morphology improving action (JP-A-11-29460), dermal collagen fiber bundle improving action (JP-A-11-12122), elastase inhibitory action (Japanese Patent No. 2969451), and the like. ing. However, the effects of the present invention obtained by using the extract or extract fraction of cuckoo and / or the extract or extract fraction of Warmoko in combination with the extract or extract fraction of malt have been described so far. Not even suggested at all.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Cuckoo ( Puerariae Radix ) used in the present invention is a root excluding the pericarp of kudzu ( Pueraria lobata Ohwi), which is a deciduous fujimoto belonging to the leguminous family ( Leguminosae ), and is produced in Japan and Korea. And square brackets, board brackets and powdered brackets produced in China can be used.

[0010] In addition, Waremokou ( Sanguisorba officina)
lis L.) is a perennial herb belonging to the family Rosaceae ,
Although various parts such as leaves, stems, flowers, and roots can be used, it is preferable to use roots.

Next, barley ( Hordeum vu) is used as malt.
lgare L.), wheat ( Triticum sativum Lamarck), rye ( Secale cereale L.), oats ( Avena fat )
ua L.), oat ( Avena sativa L.) and the like, which have germinated grains. These are Gramineae ( Gr
It is preferable to use barley malt in the first or second year herb belonging to amineae ). As barley, Rokujo barley ( Hordeum vulgare L. var. He
xastichon Aschers.), Barley ( Hordeum vu)
lgare L.var. vulgare ), barley ( Hordeum)
vulgare L. var. distichon Alefeld) and various types of barley can be used.

[0012] Cuckoo ( Puerariae Radix ), Waremokou ( Sanguisorba officinalis L.) and malt may be subjected to extraction as raw.
It is preferable to perform extraction after performing processing such as drying and pulverization. In particular, malt can be extracted by fermentation with bacteria, yeast, or the like. The extraction is performed by immersion in an extraction solvent. Stirring may be performed to increase extraction efficiency, homogenization may be performed in an extraction solvent, or hydrolysis may be performed by enzymatic treatment or the like. It is appropriate that the extraction temperature is set to a temperature of about 5 ° C. to the boiling point of the extraction solvent or lower. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but from 4 hours to
It is appropriate to set it to about 14 days.

As an extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether; esters such as ethyl acetate and butyl acetate And polar organic solvents such as ketones such as acetone and ethyl methyl ketone, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.

The extract of the above-mentioned solvents of cuckoo ( Puerariae Radix ), squid ( Sanguisorba officinalis L.) and malt can be contained as it is in the external preparation for skin according to the present invention. It may be used after being redissolved in water or a polar solvent, or subjected to a purification treatment such as decolorization, deodorization, desalting or the like within a range that does not impair these physiological actions, or after a fractionation treatment by column chromatography or the like. . The extract can be used after further fermentation or hydrolysis treatment. In particular, with respect to the extract of Puerariae Radix , the fraction eluted by 50% to 99.5% by volume of the aqueous ethanol solution when adsorbed on the synthetic adsorption resin and then sequentially eluted with a water / ethanol mixed solvent. It contains the most amount of 4 ', 7-dihydroxyisoflavone and is most preferably used in terms of action and effect. As the synthetic adsorption resin, DIAION MCI gel HP-20 (manufactured by Mitsubishi Chemical Corporation), which is an adsorption resin made of a styrene polymer, is preferably used. The above-mentioned extract of cuckoo, sprouts and malt, and its processed and fractionated products can be freeze-dried after each treatment and fractionation, and can be used by dissolving in a solvent at the time of use. Also,
It can also be used by being encapsulated in vesicles such as liposomes or microcapsules.

In the present invention, purified 4 ', 7-dihydroxyisoflavone may be used in place of the above-mentioned extract of cuckoo or a fraction thereof.

In the present invention, the parentheses ( Puerar)
iae Radix ), one or more of the extracts and extract fractions, or 4 ', 7-dihydroxyisoflavone, and / or
Or one or more kinds of extracts and extract fractions of Warmomko ( Sanguisorba officinalis L.) and one or more kinds of malt extracts and extract fractions are contained in a skin external preparation base. . The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders, granules and the like. Also, skin cosmetics such as lotion, milky lotion, cream, serum, pack, etc.
It can be provided as a base cosmetic such as a make-up base lotion and a make-up base cream, a foundation of various dosage forms such as emulsion, oily and solid forms, and a make-up cosmetic such as an eye color and a cheek color.

The external preparation for skin according to the present invention includes, in addition to an extract of cuckoo ( Puerariae Radix ) and / or an extract of Warm grub ( Sanguisorba officinalis L.) and an extract of malt, oily components, Raw materials for general pharmaceuticals and cosmetics such as surfactants, moisturizers, pigments, ultraviolet absorbers, antioxidants, fragrances, antibacterial and antifungal agents, skin cell activators, anti-inflammatory agents, whitening agents, etc. A physiologically active ingredient can be contained.

[0018]

EXAMPLES Further, the features of the present invention will be described in detail with reference to examples.

First, an extract and an extract of cuckoo ( Puerariae Radix ), an extract and an extract of warre moth ( Sanguisorba officinalis L.), and an extract of malt contained in the external preparation for skin according to the present invention. And preparation of the extract fraction.

[Cuckon extract 1] 200 g of dried powder of cuckold was immersed in 1 liter of ethanol, and allowed to stand at 20 ° C for 7 days to extract. The extract was collected by filtration, concentrated, and dried. Lyophilized to give the title parentheses extract.

[Cuckold extract 2] 500 g of cuckoo is cut into small pieces, extracted with stirring in 2 liters of ethanol at 20 ° C. for 3 days, and the extract is collected by filtration, concentrated and dried.
It was dissolved in 1 liter of glycerin to obtain the title extract of parentheses.

[Brack extract 3] 250 g of bracken are dried and pulverized, immersed in 2 liters of a 50% by volume aqueous ethanol solution at 20 ° C. for 7 days, and then filtered to collect a filtrate. did.

[Cuckon Extraction Fractions 1-4] 500 g of Puerariae Radix is dried, pulverized, and immersed in 2 liters of a 50% by volume aqueous ethanol solution at 20 ° C.
After extraction for 7 days, the filtrate was collected by filtration,
Then concentrate, dry and freeze-dry. This dry powder 3
6.0 g was dissolved in 500 ml of ethanol, 1500 ml of purified water was added, and 600 ml of DIAION M was added.
After CI gel HP-20 (manufactured by Mitsubishi Chemical Corporation) was added and stirred for 1 hour, the resin was collected by filtration, packed in a column, and eluted stepwise with a mixed solvent of water and ethanol. . At that time, 50% by volume ethanol aqueous solution, 70%
The fractions eluted with a 10% by volume aqueous ethanol solution, a 90% by volume aqueous solution of ethanol, and a 99.5% by volume aqueous solution of ethanol were collected, respectively, freeze-dried, and the title extract 1
To 4.

[0024] [burnet extract 1] Burnet (Sang
uisorba officinalis L.) was dried and pulverized, immersed in 1.5 liter of 50% by volume ethanol aqueous solution at 20 ° C. for 7 days, and then filtered to collect a filtrate.
It was designated as extract 1 of the title.

[0025] [burnet extract 2] Burnet (Sang
uisorba officinalis L.)
3-butylene glycol at 20 ° C in 2 liters
Extracted with stirring for days. The filtrate was collected by filtration to give the title extract 2.

[0026] [burnet extract 3] Burnet (Sang
uisorba officinalis L.)
The mixture was homogenized in 2.5 liter of phosphate buffered saline (pH = 7.2), centrifuged at 3,000 rpm for 10 minutes, and the supernatant was collected to obtain the title extract 3.

[0027] [burnet extract 4] Burnet (Sang
uisorba officinalis L.) was dried, pulverized, immersed in 2.5 liters of ethanol,
The mixture was allowed to stand for 0 days for extraction, and the extract was collected by filtration, concentrated, dried and then freeze-dried to obtain the title extract 4.

[Warm Mocks Extracted Fractions 1 and 2] 500 g of roots of Warm Mocks ( Sanguisorba officinalis L.) were dried, pulverized, and immersed in 2.5 liters of ethanol.
After extracting by leaving still at 20 ° C. for 7 days, the filtrate is recovered by filtration, then concentrated, dried and freeze-dried. This dry powder is dissolved in 500 ml of ethanol, and purified water 15
After adding 00 ml, 600 ml of DIAION MCI gel HP-20 (manufactured by Mitsubishi Chemical Corporation) was added, and the mixture was stirred for 1 hour. Then, the resin was collected by filtration, packed in a column, and mixed with a mixed solvent of water and ethanol. And eluted step by step. At this time, the fractions eluted with a 30% by volume aqueous ethanol solution and a 50% by volume aqueous ethanol solution were collected and freeze-dried to obtain fractionated extracts 1 and 2, respectively.

[0029] [malt extract 1] six Zhou barley (Ho
rdeum vulgare L. var. hexastichon Aschers.) was crushed and extracted with stirring in 2 liters of a 50% by volume aqueous ethanol solution at 20 ° C. for 3 days, followed by filtration to collect the filtrate and extract the title extract 1 And

[Malt extract 2] Wheat ( Triticum sativ)
um Lamarck) was immersed in 2.5 liters of hot water and extracted for 4 hours, and then filtered to collect a filtrate.
It was designated as title extract 2.

[Malt extract 3] Rye ( Secale cerea)
le L.) was homogenized in 2 liters of physiological saline at 10 ° C., and then stirred for 5 hours.
The mixture was centrifuged at 3,000 rpm for 10 minutes, and the supernatant was recovered to obtain the title extract 3.

[Malt extract 4] Hordeum barley ( Hord)
eum vulgare L.var. vulgare) malt 500g was immersed in purified water in a 2.0-liter, stirred 2 hours at 40 ° C. to 45 ° C., the pH was adjusted to 5.5-6.0, lactic acid bacteria ( Lactobacillus delbruckii ), and add 45 ℃ -50 ℃
Fermented anaerobically for 5 days. Then, the mixture was centrifuged at 3,000 rpm for 10 minutes, and the supernatant was recovered and filtered with a Millipore filter to obtain the title extract 4.

[Malt Extract Fractions 1 and 2] Hokumou barley ( Hordeum vulgare L. var. Hexastichon Ascher)
s.) is dried and pulverized, immersed in 2.5 liters of a 20% by volume aqueous ethanol solution, allowed to stand at 20 ° C. for 7 days, extracted, and then filtered to collect a filtrate. To this filtrate, 600 ml of DIAION MCI gel HP-2
After adding 1 (manufactured by Mitsubishi Chemical Corporation) and stirring for 1 hour, the resin was recovered by filtration, packed in a column, and mixed with water and water.
Elution was carried out stepwise with a mixed solvent of ethanol. At this time, the fractions eluted with a 15% by volume aqueous solution of ethanol and a 30% by volume of aqueous solution of ethanol were collected and freeze-dried to obtain fractionated extracts 1 and 2, respectively.

Next, the formulations of the examples of the external preparation for skin according to the present invention will be shown.

[Example 1] Lotion agent (1) Ethanol 20.00 (% by weight) (2) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.00 (3) Cuckold extract fraction 10 02 (4) Methyl paraoxybenzoate 0.10 (5) Dipropylene glycol 5.00 (6) 1,3-butylene glycol 10.00 (7) Malt extract 10.20 (8) Purified water 63.68 Manufacturing method: (2) to (4) are added to (1) and dissolved to form an alcohol phase. On the other hand, (5) to (7) are sequentially dissolved in (8) to form an aqueous phase. Add the alcohol phase to the aqueous phase, stir and mix.

Example 2 Emulsion (1) Cetanol 1.00 (% by weight) (2) Beeswax 0.50 (3) Vaseline 2.00 (4) Squalane 6.00 (5) Dimethylpolysiloxane 2.00 (6) polyoxyethylene (20E.O.) sorbitan 1.00 monostearate (7) glyceryl monostearate 1.00 (8) glycerin 4.00 (9) 1,3-butylene glycol 4.00 (10) Methyl paraoxybenzoate 0.10 (11) Purified water 62.95 (12) Carboxyvinyl polymer 10.00 (1.0% by weight aqueous solution) (13) Potassium hydroxide 0.10 (14) Ethanol 5. 00 (15) Crack extract extract 1 0.05 (16) Malt extract 2 0.30 Production method: The oil phase components of (1) to (7) were mixed and dissolved by heating.
° C. On the other hand, the aqueous phase components (8) to (11) are mixed and dissolved to 75 ° C. The oil phase was added thereto, and the mixture was pre-emulsified. (12) was added, and the mixture was uniformly emulsified with a homomixer.Then, (13) was added to increase the viscosity, followed by cooling. To
Dissolve in (14), add and mix with (16).

[Example 3] Oil-in-water cream (1) Beeswax 6.00 (wt%) (2) Cetanol 5.00 (3) Reduced lanolin 8.00 (4) Squalane 27.50 (5) Glyceryl Fatty acid ester 4.00 (6) Lipophilic glyceryl monostearate 2.00 (7) Polyoxyethylene (20E.O.) sorbitan 5.00 Monolaurate (8) Propylene glycol 5.00 (9) Paraoxy Methyl benzoate 0.10 (10) Cuckoo extract fraction 2 0.02 (11) Warm grub extract 2 0.25 (12) Malt extract 3 0.25 (13) Purified water 36.88 Production method: (1) ) To (7) are mixed and dissolved to reach 75 ° C. On the other hand, the aqueous phase components (8) to (13)
Heat to ° C. Next, the oil phase component is added to the aqueous phase component, preliminarily emulsified, uniformly emulsified by a homomixer, and cooled.

Example 4 Gel (1) Dipropylene glycol 10.00 (% by weight) (2) Carboxyvinyl polymer 0.50 (3) Potassium hydroxide 0.10 (4) Methyl paraoxybenzoate 10 (5) Cuckoo extract fraction 3 0.02 (6) Crackworm extract 4 0.05 (7) Malt extract 4 0.15 (8) Purified water 89.08 Production method: (8) to (2) After dissolving uniformly, (4) to (7) are dissolved and added to (1), and then (3) is added to increase the viscosity.

[Example 5] Oil-in-water emulsion ointment (1) White petrolatum 25.00 (wt%) (2) Stearyl alcohol 25.00 (3) Glycerin 12.00 (4) Sodium lauryl sulfate 1.00 (5) Methyl paraoxybenzoate 0.10 (6) Purified water 36.20 (7) 1.0% (w / v)% 0.15 ethanol solution of the parenthesized extract 4 (8) Warmoko extract 10 .20 (9) Warmberry extract 3 0.20 (10) 1.0 (w / v)% aqueous solution of malt extract fraction 1 0.15 Production method: mixing oil phase components (1) to (4) , Heat to uniformly dissolve and bring to 75 ° C. On the other hand, the aqueous phase components of (5) and (6) are mixed,
Heat to 75 ° C. The oil phase component was gradually added to the water phase component with stirring to emulsify, and after cooling, 40
Add (7) to (10) at ℃ and mix.

Example 6 Liposome preparation (1) Glycerin 2.0 (% by weight) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25E.O.) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 79.7 (7) 4 ', 7-dihydroxyisoflavone, Waremokou 5.0 Extractable fraction 2, Malt extract fraction Product 2: Encapsulated liposome Manufacturing method: Dissolve (5) in (4), add to (6) together with (1) to (3), mix uniformly, add (7) to this, and disperse. The liposome of (7) was prepared by adding 1.0% (w / v) of 4 ′, 7-dihydroxyisoflavone and 2.5%
(w / v)% and malt extract fraction 2 in 100 ml of a 50% by volume ethanol aqueous solution containing 2.5 (w / v)%, 80 g of soybean lecithin was added and suspended at 55 ° C. A liposome was prepared by treatment and collected by centrifugation.

Example 7 Water-in-oil emollient cream (1) Liquid paraffin 30.00 (% by weight) (2) Microcrystalline wax 2.00 (3) Vaseline 5.00 (4) Diglyceryl dioleate Ester 5.00 (5) Sodium L-glutamate 1.60 (6) L-Serine 0.40 (7) Propylene glycol 3.00 (8) Methyl parahydroxybenzoate 0.10 (9) Cuckoo extract 10 02 (10) Malt extract 4 0.05 (11) Purified water 52.73 (12) Fragrance 0.10 Production method: (5), (6) is dissolved in a part of (11) to 50 ° C. It is gradually added to (4), which has been heated to 50 ° C. in advance, with stirring. This is mixed in advance, and is uniformly dispersed in (1) to (3) heated and dissolved at 70 ° C. In addition, (8), (9)
And added to the remainder of (11) together with (10), and the mixture heated to 70 ° C. is added with stirring and emulsified by a homomixer. After cooling, (12) is added and mixed at 40 ° C.

Example 8 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self-emulsification Glyceryl monostearic acid 2.0 ester (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Methyl parahydroxybenzoate 0.1 (8) Cuckon extract 2 0.1 (9) Purification Water 68.2 (10) Titanium oxide 2.0 (11) Bengala 0.4 (12) Yellow iron oxide 0.1 (13) Fragrance 0.1 (14) Malt extract 10.2 Production method: (1) Mix and dissolve the oil phase components of (4) to (75). On the other hand, the aqueous phase components (5) to (9) are mixed and dissolved by heating, and the pigment components (10) to (12) are added thereto and uniformly dispersed by a homomixer to 75 ° C. Next, the oil phase component is added to the water phase component, and the mixture is uniformly emulsified by a homomixer. After cooling, (13) and (14) are added and mixed at 40 ° C.

Example 9 Emulsion Foundation (1) Stearic acid 2.00 (% by weight) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitate 9.00 (6) 1,3-butylene crychol 6.00 (7) Potassium hydroxide 0.08 (8) Methyl parahydroxybenzoate 0.10 (9) Warmoko extract 40 0.05 (10) Malt extract 3 0.10 (11) Purified water 53.32 (12) Titanium oxide 9.00 (13) Talc 7.40 (14) Bengala 0.50 (15) Yellow iron oxide 10 (16) Black iron oxide 0.10 (17) Fragrance 0.15 (18) Cuckoo extract 3 0.10 Production method: Mix and dissolve oil phase components (1) to (5) to 75 ° C . On the other hand, the aqueous phase components (6) to (11) are mixed and dissolved by heating.
The pigment components (12) to (16) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the water phase component, and the mixture is uniformly emulsified by a homomixer. After cooling, (17) and (18) are added and mixed at 40 ° C.

Example 10 Hand cream (1) Cetanol 4.00 (% by weight) (2) Vaseline 2.00 (3) Liquid paraffin 10.00 (4) Glyceryl monostearate 1.50 (5) Polyoxyethylene (60E.O.) glyceryl 2.50 Isostearate (6) Tocopherol acetate 0.25 (7) Glycerin 20.00 (8) Methyl parahydroxybenzoate 0.10 (9) Warmoko extract extract 1 0.03 (10) Malt extract fraction 1 0.03 (11) Purified water 59.59 Production method: Mix and dissolve oil phase components (1) to (6) to 75 ° C. On the other hand, the aqueous phase components (7) to (11) are mixed and dissolved by heating to 75 ° C. Next, the oil phase component is added to the aqueous phase component, uniformly emulsified by a homomixer, and cooled.

Among the examples according to the present invention described above, Examples 1 to 6 were evaluated for the effect of suppressing the formation of wrinkles on the skin by medium wavelength ultraviolet (UVB). At that time, with respect to the extract or fraction of cuckoo extract, scallop extract or malt blended in each of Examples 1 to 5, a part of these was replaced with purified water, and blended in Example 6. 4 ', 7-dihydroxyisoflavone,
The liposomes containing the extract fractions of Warmoko and Malt were also substituted to Comparative Examples 1 to 6, and evaluated simultaneously. The extracts and the like contained in Comparative Examples 1 to 6 are as shown in Table 1. For evaluation, five hairless mice were used as one group, and the examples and comparative examples were applied to the back once a day for each group, and 100 mJ / cm.
² / times UVB was irradiated three times a week for 20 weeks, and the formation of wrinkles on the skin of hairless mice was observed.
The evaluation was made by scoring according to the criterion shown in (1). On this occasion,
A group to which only purified water was applied was used as a control. The results were calculated as the average of each group, and are shown in Table 3 in relation to the number of UVB irradiation days.

[0046]

[Table 1]

[0047]

[Table 2]

[0048]

[Table 3]

As is clear from Table 3, in the control, the depth of wrinkles formed on the skin reached a medium level when the number of UVB irradiation days exceeded 10 weeks, and deep wrinkles formed after 20 weeks. Was recognized. On the other hand, in the group applied with the example of the present invention, a significant effect of suppressing formation of wrinkles was observed in all groups, and after 20 weeks, the fine wrinkles were applied in the group applied in Example 1 and in the group applied in Examples 3 to 6. In the application group of Example 2, slight wrinkles were formed.
On the other hand, Comparative Example 1 containing an extract of cuckoo, and
In the group coated with Comparative Example 6 containing liposomes containing only 4 ', 7-dihydroxyisoflavone, the formation of minor wrinkles was observed only after 20 weeks, and the formation of wrinkles was well suppressed. Was smaller than each corresponding Example application group. In Comparative Example 2 and Comparative Example 5 applied groups containing the extract or extract fraction of Warmoko, wrinkle formation was considerably suppressed, but the degree was smaller than that of each corresponding Example applied group. After all it was small. In Comparative Example 3 and Comparative Example 4 application groups, formation of wrinkles near medium was observed after 20 weeks, and the effect of suppressing wrinkles was small.

Subsequently, use tests were conducted on Examples 1 to 10 of the present invention to evaluate the effect of improving the aging symptoms of the skin. At that time, the cuckoo extract and Warmoko, malt extract or extract fraction blended in Examples 7 to 10 were replaced with Comparative Examples 7 to 10 and along with Comparative Examples 1 to 6 above. At the same time, it was subjected to a use test. Table 4 shows extracts and the like contained in Comparative Examples 7 to 10.
It was shown to.

[0051]

[Table 4]

The effect of ameliorating the aging of the skin is in the range of 40's to 60's, in which fine wrinkles are formed and skin elasticity is remarkably reduced.
Twenty-year-old female panelists were set as one group, and each group was evaluated using each of the Examples and Comparative Examples twice a day blindly for two consecutive months. The degree of fine wrinkles was evaluated by visual observation and photography, and the skin elasticity was measured with a cute meter. The conditions before and after the start of the use test were compared. None ". The results are shown in Table 5 by the number of panelists who obtained each evaluation.

[0053]

[Table 5]

As is evident from Table 5, in the group using the examples of the present invention, good wrinkles and improvement in skin elasticity were observed in all the groups, and particularly in the groups using the examples 3 to 6. The effect was remarkable. On the other hand, wrinkles and skin elasticity were observed in the comparative group containing only the cuckoo extract or a fraction thereof, or 4 ′, 7-dihydroxyisoflavone, a waremokou extract or a fraction thereof, or a malt extract. Although the tendency of improvement was observed, the number of panelists who recognized a clear improvement was significantly less than the corresponding groups using the examples.

Further, the effects of the present invention on improving skin roughness symptoms were evaluated for Examples 1 to 10 and Comparative Examples 1 to 10. The effect of improving the skin roughness symptoms was as follows. Twenty female panelists in their twenties to sixties who exhibited remarkable skin roughness symptoms were grouped into one group, and each group was blinded with each of the Examples and Comparative Examples twice daily. The evaluation was performed by using continuously for months. The skin condition before and after the start of the use test was evaluated and scored according to the evaluation criteria shown in Table 6, and the average value of 20 persons was calculated and shown in Table 7.

[0056]

[Table 6]

[0057]

[Table 7]

As is evident from Table 7, in each of the groups using the examples of the present invention, remarkable improvement in skin roughness was observed, and the skin condition was improved to a satisfactory state after the end of the use test. On the other hand, even in the comparative example use group, quite good improvement of skin roughness was recognized,
The degree was smaller than that of the corresponding group using the example.

Note that in Examples 1 to 10, 2
When stored at 5 ° C. for 6 months, no change in the condition was observed, and no problematic skin irritation was observed in a 48-hour back obstruction sticking test by 30 male panelists.

[0060]

As described in detail above, according to the present invention, an external preparation for skin having good stability and safety, and synergistically improved prevention and improvement of skin roughness and skin aging can be obtained. .

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/00 A61K 7/00 D 31/352 31/352 A61P 17/00 A61P 17/00 29/00 29 / 00 // C07D 311/36 C07D 311/36 F term (reference) 4C062 EE44 4C083 AA082 AA111 AA112 AB032 AB232 AB242 AB432 AC012 AC022 AC072 AC102 AC122 AC182 AC242 AC352 AC422 AC432 AC442 AC482 AC582 AC841 AC842 AD092 CC152 CC CC12 DD23 DD31 DD32 DD33 DD41 DD45 EE10 EE12 EE13 4C086 AA01 AA02 BA08 MA03 MA04 MA63 NA14 ZA89 ZB11 4C088 AB51 AB59 AB73 AB75 AC03 AC04 AC05 AC11 BA08 BA14 BA37 CA14 MA07 MA08 MA63 NA14 ZA89 ZB11

Claims (3)

[Claims] 1. One or more selected from extracts and extract fractions of cuckoo ( Puerariae Radix ), and / or
Or one or more selected from extracts and extract fractions of Warm grub ( Sanguisorba officinalis L.) and one or more selected from malt extracts and extract fractions , External preparation for skin. 2. When the extract of cuckoo ( Puerariae Radix ) is adsorbed on a synthetic adsorption resin and subsequently eluted with a mixed solvent of water and ethanol, 50% by volume to 9% by volume.
The skin external preparation according to claim 1, wherein the fraction is a fraction eluted with a 9.5% by volume aqueous ethanol solution. 3. One or more selected from 4 ′, 7-dihydroxyisoflavone and / or an extract and extract fraction of Warm Mob ( Sanguisorba officinalis L.),
An external preparation for skin comprising one or more selected from malt extracts and extract fractions.