JP2000198712A - Cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cosmetic composition having anti-tyrosinase activity, exhibiting excellent skin whitening effect and moisture retaining effect by making the composition include Platycodon grandiflorum or its extract. SOLUTION: This composition contains preferably 0.001-20 wt.%, calculated as solid content (the solid content of a plant used in the extraction in the case of an extract) based on the whole composition, of Platycodon grandiflorum (perennial of the family Campanulaceae and the place of production is Hokkaidou, Miyagi, Iwate, Nagoya Prefectures, or the like). The extract is obtained by extraction using a solvent such as a hydrophilic organic solvent e.g. water or an alcohol or its mixed liquid suitably extracting the active ingredient of Platycodon grandiflorum [e.g. compounds shown by formula I to formula III] by a cold immersion method, a warm immersion method, or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition, and its object is to exhibit an excellent whitening effect by having an anti-tyrosinase activity and to suppress or treat pigmentation such as spots, freckles, and black and white. It is an object of the present invention to provide a cosmetic composition which can provide fair and beautiful skin by using the composition, and can provide moisture to the skin by having a moisturizing effect.

[0002]

2. Description of the Related Art Various components are blended in cosmetics according to the purpose. For example, whitening agents are incorporated in many cosmetics for the purpose of improving, alleviating or preventing pigmentation such as spots, freckles, and black and white, and suppress the activity of tyrosinase to cause the cause of pigmentation. Has the function of suppressing the deposition of the melanin pigments on the skin. Also,
In recent years, since many people prefer a fair skin, many cosmetics, such as a whitening milky lotion and a whitening lotion, have been created which claim the effect of a whitening agent. As this whitening agent, ascorbic acid, kojic acid, arbutin, glutathione, sulfur preparations and the like have been conventionally used.

[0003] A moisturizing agent is also one of the components blended in many cosmetics, and is blended in order to provide moisture to the skin that tends to dry and maintain freshness. That is, if the moisturizing property of the skin decreases due to aging or other causes, the skin becomes dry and loses the flexibility and elasticity of the skin, and as a result, the skin may become rough or wrinkles may occur. Moisturizers are included to prevent and maintain healthy skin. As the humectant, glycerin, propylene glycol, 1,3-butylene glycol, polyhydric alcohol, pyrrolidone carboxylic acid, saccharides, amino acids, collagen and the like have been conventionally used.

[0004]

However, the conventional whitening agents and moisturizing agents have the following problems. That is, conventionally used whitening agents are not satisfactory in their whitening effect, and glutathione and sulfur preparations have a peculiar off-odor, and precipitates when mixed with water-soluble cosmetics. There is also a problem that it may occur. Therefore, such whitening agents cannot be used widely as additives for cosmetics. On the other hand, moisturizing agents that have been used in the past have the problem of increased skin damage due to their formulation and low safety on the skin. For example, glycerin
Since it has not only a moisture-retaining ability but also a moisture-absorbing ability, it has a drawback that the water in the stratum corneum is deprived, causing skin inflammation and darkening of the entire skin.

[0005] Therefore, it is desired to create a whitening agent which has a high whitening effect and can be widely used as an additive for cosmetics, and a moisturizing agent which has a high moisturizing property and is excellent in safety. After continuing intensive research on whitening agents and moisturizers that are highly safe and originated, Kikyo (Platyco
don grandiflorum A.DC.) and its extract have both a whitening effect and a moisturizing effect, and have completed the present invention.

[0006]

Means for Solving the Problems That is, the present invention according to claim 1 is characterized by the fact that the present invention relates to a fennel (Platycodon grandiflorum A.DC.) and / or
Or a cosmetic composition comprising an extract thereof.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The cosmetic composition according to the present invention comprises:
Kikyo (Platycodon grandiflorum A.DC.) is used. Kikyo (Platycodon grandiflorum A.DC.) is a perennial herbaceous family and is produced in Hokkaido, Miyagi, Iwate, and Nagano prefectures of Japan, as well as in China and the Korean Peninsula. The height is 1m, the roots are thick and the stems are branched and upright. The leaves are 4 to 6 cm long, and bluish purple to white flowers bloom from summer to autumn.

[0008] Kikyo (Platycodon grandiflorum AD)
It is known that the roots of C.) contain triterpenoid saponins and sterols. Inulin is used as a saccharide, and the following formula 1 is used as a sapogenin.
Polygalacicacid represented by the following formula (1), the following formula (2)
Platycodinin represented by the following formulas 3 (formula 3) to platycogenic acids A, B, C represented by the following formula 5 (formula 5), and the following formula 6
Platycogenin represented by (Chemical formula 6) is converted into 3-O-β-glucosylplatyco represented by the following formula 7 (Chemical formula 7) as prosapogenin.
Digenin is α-spinasterol represented by the following formula 8 (formula 8) as a sterol, and Δ ⁷ -s represented by the following formula 9 (formula 9)
tigmastenol, α-spinast represented by the following formula 10
eryl-β-D-glucoside, represented by the following formula 11
betulin is isolated.

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[0009] Kikyo (Platycodon grandiflorum AD)
The pharmacological action of C.) has been proven to be sedative, analgesic, antipyretic and anti-inflammatory, as well as antitussive, hypotensive and anticholinergic. It is used as an antitussive for cough and bronchitis. It has also been applied as a purulent drug to purulent diseases, tonsillar circles, sore throat and the like.

In the present invention, the above-mentioned fennel (Platyco
don grandiflorum A.DC.) can be used in all areas, above and below the ground, using whole grass, or using individual parts such as leaves, roots, stems, etc., alone or as appropriate You may.

Kikyo (Platycodon grandiflorum AD)
The form of use of C.) is not particularly limited, and it may be used as a powder or as an extract. Also,
These may be used in combination.

[0012] The extraction solvent used for obtaining the extract is not particularly limited, and may be a hydrophilic organic solvent such as water or alcohol, or a mixture thereof, such as Platycodon grandi.
florum A.DC.) can be suitably used as long as it is a solvent from which the active ingredient can be suitably extracted. In addition, as a hydrophilic organic solvent, monohydric alcohols, such as methanol and ethanol, polyhydric alcohols, acetone, etc. can be illustrated. Examples of the extraction method include a cold immersion method and a hot immersion method, but are not particularly limited.

The extract obtained by the above method may be used as it is, but it can be concentrated and used as a soft extract, or dried by freeze-drying or spray-drying and used as a dry extract.

In the cosmetic composition, the fennel (Platycodon gr)
andiflorum A.DC.) is not particularly limited, but the solid content (in the case of an extract, the solid content of the plant used for extraction) is 0.001 to 20% by weight, more preferably 0. The content is desirably from 0.01 to 5% by weight.

The cosmetic composition having the above constitution has an anti-tyrosinase activity and a moisturizing effect, as is clear from the test examples described later. Therefore, since it exhibits an excellent whitening effect due to its anti-tyrosinase activity, it can be used for the suppression or treatment of pigmentation such as spots, freckles, and black and white, and it can be a fair and beautiful skin, and its moisturizing. The effect moisturizes the skin and keeps the skin fresh.

The dosage form of the cosmetic composition according to the present invention is not particularly limited. In addition to ordinary cosmetics, its anti-tyrosinase activity is used to make it possible to use the sunburn to cause pigmentation such as spots, freckles, and black and white caused by sunburn. It can also be used as a medicated cosmetic (quasi-drug) with a specific use purpose such as prevention of inflammation and recovery of skin after sunburn, or as a medicated external preparation for the treatment of skin irritation caused by sunburn. it can. Specifically, lotions, creams, emulsions, foundations, oily cosmetics, packs,
Cosmetics such as skin cleanser and medicated cosmetics (quasi-drugs),
It can be used as a medicated external preparation such as an ointment, liniment and lotion.

In the present invention, the above-mentioned Kikyo
In addition to (Platycodon grandiflorum A.DC.) and / or an extract thereof, components to be blended in a usual cosmetic composition, for example, oils, surfactants, ultraviolet absorbers, medicinal components, alcohols, preservatives, pigments , Fragrance and the like can be appropriately compounded within a range that does not impair the effects of the present invention.

[0018]

EXAMPLES Hereinafter, the present invention will be described based on Examples and Comparative Examples.
This will be described in detail. However, the present invention is not limited to these examples. (Examples 1-2) Kikyo (Platycodon grandiflorum)
A. DC.) Was immersed in warm water at 60 ° C. for about 5 hours and filtered. After repeating this several times, the obtained warm water extracts were combined and concentrated under reduced pressure, and the obtained extract 6.5 was obtained.
g was used as the sample of Example 1. Kikyo (Platycodon)
grandiflorum A.DC.) was similarly extracted and concentrated under reduced pressure using 100 g of the root, and 6.4 g of the obtained extract was used as a sample of Example 2.

(Examples 3 and 4) Platycodon gr
andiflorum A.DC.) was used as a sample in Example 3 and dried (Platycodon grandiflorum AD).
100 g of the dried powder of the root of C.) was used as the sample of Example 4.

(Comparative Examples 1 and 2) Standard samples of arbutin were used as samples of Comparative Example 1, and standard products of glycerin were used as samples of Comparative Example 2.

[0021]

Test Examples The following tests were performed using the samples of Examples 1 to 4 and Comparative Examples 1 and 2. (Test Example 1: Measurement of anti-tyrosinase activity) 10% FBS
-B16 melanoma 4A5 (RIKEN cell bank) cultured in DMEM was seeded in a 96-well plate at a concentration of 5 x 10 ⁴ cells / well (total volume: 195 µl), and was incubated at 37 ° C and 5% -CO.
The cells were cultured under the conditions of ₂ for 24 hours. Here, the final concentration of each of the samples of Examples 1 to 4 and Comparative Example 1 was 5, 10, 5 respectively.
The mixture was diluted with a 50% ethanol / PBS solution to a concentration of 0, 100, and 500 ppm, and sterilized by filtration through a 0.2-μm membrane filter. After the culture, the culture solution was removed, and 1% Triton X-100 / PBS solution 5
After solubilizing the cell membrane with 0 μl, 10 mM
100 μl of L-DOPA was added and reacted at 37 ° C. for 3 hours, and the wavelength was 475 nm using a microplate reader.
Was measured. Also, as a control, the absorbance of the reaction system when no sample solution was added was measured.
The tyrosinase biosynthesis inhibition rate (%) was calculated based on (Equation 1).

(Equation 1) A: Absorbance without sample solution added B: Absorbance with sample solution added

The results are shown in Table 1.

[Table 1]

(Test Example 2; Moisturizing Effect Test)
Samples of No. 4 and Comparative Example 2 were dissolved in ethanol to prepare a 1.0% by weight sample solution, and 5 ml of each sample solution was collected in a weighing bottle. The weighing bottle was left for 12 hours under the conditions of a humidity of 60 to 70% and a temperature of 23 to 25 ° C., and the moisture evaporation rate was examined. In addition, water was used as a control.

The results are shown in Table 2.

[Table 2]

(Test Example 3; Moisturizing Effect Test) Using the samples of Examples 1 to 4 and Comparative Example 2 as humectants, five kinds of creams were prepared according to the following composition. Composition Blending amount (% by weight) Moisturizer 2.0 Beeswax 6.0 Ethanol 5.0 Reduced lanolin 8.0 Squalane 37.5 Perfume Appropriate amount Antioxidant Appropriate amount Propylene glycol 5.0 Purified water Remaining total 100. 0

Women with dry skin and rough skin 50
Names were panelists and divided into five groups of ten. Each of the five groups was a group using the above-mentioned five types of creams, and was applied 3 to 5 times a day to a site having rough skin after water work. One month later, the degree of improvement in skin roughness and moist feeling were scored on the following four levels. Skin roughness improvement effect 1 ... worse than before use 2 ... almost the same as before use 3 ... slightly improved compared to before use 4 ... moist feeling significantly improved compared to before use 1 ... use Deteriorated compared to before 2 ... almost the same as before use 3 ... slightly moist compared to before use 4 ... fairly moist compared to before use

Table 3 shows the result of calculating the average value of the points of 10 persons.
Shown in

[Table 3]

From the results shown in Tables 1 to 3, it can be seen that Kikyo (Platycodo
n grandiflorum A.DC.) or a cosmetic composition containing the extract thereof has an anti-tyrosinase activity;
Moreover, it can be seen that the moisturizing effect is high. Therefore, the cosmetic composition according to the present invention exhibits an excellent whitening effect due to the anti-tyrosinase activity, can be effectively used as a cosmetic for whitening, and has pigmentation such as spots, freckles, and black and white. It can also be used to control or treat the disease. In addition, the skin can be moisturized by its excellent moisturizing properties.

Hereinafter, examples of the composition of the cosmetic composition according to the present invention will be described. (Formulation Example 1; lotion) Composition Mixing amount (% by weight) Extract of Example 1 1.5 Squalane 5.0 Vaseline 2.0 Beeswax 0.5 Sol Bitane sesquioleate (20E.O) 8 Propylene glycol 5.0 Ethanol 5.0 Potassium hydroxide 0.1 Antioxidant qs perfume qs Purified water balance 100.0

(Formulation Example 2: Emulsion) Composition Blending amount (% by weight) Extract of Example 2 1.5 Squalane 5.0 Vaseline 2.0 Sol Bitane sesquioleate 0.8 Polyoxyethylene oleyl ether ( 20E.O) 1.2 Propylene glycol 5.0 Ethanol 3.0 Carboxyvinyl polymer (1% aqueous solution) 18.0 Potassium hydroxide 0.1 Preservative 0.3 Perfume Appropriate amount Purified water Residual total 100. 0

[0031]

[0032]

As described above in detail, the invention according to claim 1 is characterized by the fact that the present invention relates to a Japanese fennel (Platycodon grandiflorum A.DC.) and / or
Or a cosmetic composition characterized by containing an extract thereof, exhibits a whitening effect by anti-tyrosinase activity, can be effectively used as a cosmetic for whitening, It can be used for suppressing or treating pigmentation such as spots, freckles, and black and white. Furthermore, it has an excellent effect of moisturizing the skin due to its moisturizing effect, and enabling the skin to be fresh.

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Claims (1)

[Claims] [Claim 1] Kikyo (Platycodon grandiflorum AD)
C.) and / or an extract thereof.