JPH09157151A - Melanin generation inhibitor and whitening agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a melanin generation inhibitor having excellent melanin generation inhibiting effect and high safety and stability, containing a specific plant extracted substance. SOLUTION: This melanin generation inhibitor is obtained by mixing one kind or more than two kinds of extracts of Hypericum erectum and Hypericum ascyron as perennial plants in the family Clusiaceae. The extraction is performed to preferably whole herb of the plant in a usual method by using a hydrophilic solvent (e.g. ethanol). A whitening agent is prepared by mixing 0.001-20wt.% of the melanin generation inhibitor. The whitening agent has excellent whitening action, safety and stability, and can be made to a skin preparation for external use for whitening agent and a skin cosmetic for whitening in a shape of lotion, milky agent, cream and ointment, etc.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a melanin production inhibitor having a high effect, high safety and stability, which comprises a specific plant extract, and a whitening agent comprising the same.

[0002]

2. Description of the Related Art Conventionally, whitening cosmetics have been of great interest in improving skin darkness, dark spots, freckles, and the like. In these, ascorbic acid, glutathione, colloidal sulfur and the like are effective. It has been formulated as an ingredient.

There are also examples in which various medicinal plant extracts, plant-derived gallic acid, geraniin and the like are used. further,
Whitening ingredients such as kojic acid and arbutin, a glucopyranoside derivative, have also been used recently. Such a whitening component is a tyrosinase activity inhibitor that inhibits the activity of tyrosinase that catalyzes melanin production, a melanin biosynthesis inhibitor that cleaves part or all of the process of producing melanin from tyrosine via dopa, dopaquinone, and dopachrome, melanin. Known are melanin excretion enhancers that normalize the metabolism of melanin.

[0004]

However, ascorbic acid is liable to be oxidized and unstable, and glutathione and colloidal sulfur have drawbacks of producing a peculiar odor and precipitation.

In addition, the conventional medicinal plant extracts have problems that the effect is insufficient and the quality is not constant. Furthermore, maintenance of the stability of kojic acid and the like had to be considered. In addition, there were some that caused side effects due to continuous use.

The present invention is intended to solve such problems and provide a whitening agent which is excellent in whitening effect and which is highly safe and stable.

[0007]

Since a whitening agent, especially a whitening cosmetic, is continuously used every day, the active ingredient to be added thereto has a mild action and shows sufficient effects by continuous use, Those without side effects due to are desired.
From this point of view, a substance derived from a natural product, particularly an extract of a medicinal plant is preferable, but the effectiveness of the above-mentioned action,
In order to solve problems such as stability of quality, we screened among plant extracts for effective and mild skin lightening effect and high stability.

As a result, Hypericum erect
um Thunb. or Hypericum perforatum L.), Tomoeso
In the extract of ( Hypericum ascyron L.), a high melanin production inhibitory action was found. These extracts had no adverse effects on the skin such as skin irritation and contact sensitization, and when incorporated into cosmetics, the melanin production inhibitory effect was not inactivated and the quality was stable. .

Hypericum erectum Thunb.
Or Hypericum perforatum L.) and Tomoesou ( Hypericum perforatum L.)
um ascyron L.) is a perennial plant of the family Hypericum ( Guttiferae ). Hypericum ( Hypericum erectum Thunb.)
(Hereinafter, it is abbreviated as Hypericum E) is a herbal medicine that is distributed in Japan, Korea, etc., and this whole plant is dried, and it is a herbal medicine called syringa L. ing. Hypericum perforatum L. (hereinafter referred to as Hypericum P) is mainly distributed in Europe and is used as an antidiarrheal containing tannin and Hypericin as active ingredients. (Encyclopedia of medicinal plants Hirokawa, Hirokawa Shoten, 1974)

These Hypericum perforatum E, Hypericum perforatum P,
The extraction solvent when obtaining the extract of Tomoesou is not particularly limited as long as it is an extraction solvent applied to a plant extract to be blended in a normal whitening agent and cosmetics, but a hydrophilic solvent, particularly a hydrous lower alcohol is preferable. Used. Lower alcohols include methanol, ethanol, propanol,
Examples of isopropanol include ethanol, with ethanol being particularly preferred. Alternatively, a water extract or an organic solvent extract such as acetone or hexane may be used. Further, the extraction site is not particularly limited, such as whole grass, flowers, roots, etc., but whole grass extract is particularly preferably used. When extracting, either dried or fresh plants may be used.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, the compounding amount of the melanin production inhibitor comprising the above plant extract in the whitening agent is
0.001 to 20% by weight is suitable. Compounding amount 0.0
If the amount is less than 01% by weight, a sufficient whitening effect cannot be obtained, but since the whitening effect is quite strong, it is not necessary to add a large amount, and if it exceeds 20% by weight, the stability of the whitening agent will be affected. Sometimes.

In the present invention, a whitening agent can be provided by blending the above-mentioned melanin production inhibitor, but the whitening agent can be in the form of a lotion, emulsion, cream, ointment or the like. Furthermore, softening lotion, astringent lotion, lotion such as washing lotion, emollient cream, moisturizing cream, massage cream, cleansing cream, cream such as makeup cream, emollient emulsion, moisturizing emulsion, nourishing Emulsions, cleansing emulsions and other emulsions, jelly packs,
It can also be provided as whitening cosmetics in various formulations, such as packs such as peel-off packs, wash-off packs, powder packs, serums, and face wash.

In the present invention, other active ingredients such as moisturizers, anti-inflammatory agents, and ultraviolet absorbers can also be used in combination, such as sunscreen cosmetics, skin protecting cosmetics, and rough skin improving cosmetics. It can also be provided as a cosmeceutical or a quasi drug.

[0014]

[Function] The effect of suppressing melanin production in the present invention was evaluated. The evaluation was performed as follows.

First, 10 g of dried plant pieces was added to 50% by weight.
It was put in 100 ml of ethanol and extracted at room temperature for 1 week. This plant extract was filtered and then diluted 100-fold with 50% by weight ethanol to prepare a sample solution. This sample solution was added to a mouse-derived melanoma cell line to a final concentration of 2 to 50 μg / ml and cultured for 3 days. Culture has 5 cells
It started from about 000. Separate the cells, 650 nm and 4
The absorbance at 00 nm was measured and designated as A6 and A4, and the number of cells n was counted. As a control, the cells were cultured in a system containing no plant extract, and the absorbances A′6 and A′4 of the cells at 650 nm and 400 nm and the cell number n ′ were measured. The melanin production suppression rate was calculated by the following equation (1).

(Equation 1)

Table 1 shows the measurement results of the melanin production inhibitory rate. As is clear from Table 1, each of the plant extracts used in the present invention has a significantly high melanin production inhibitory rate and exhibits an effective whitening effect.

[Table 1]

[0017]

EXAMPLES Further, the features of the present invention will be described in detail with reference to examples.

[Example 1] Liquid external preparation for skin (1) Glycerin 5.0 (% by weight) (2) Propylene glycol 4.0 (3) Ethanol 10.0 (4) Hypericum P. 50% ethanol extract 0 5 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 80.4 Dissolve (5) in (3) and add to (6), then add (1), (2) and (4) in order. Add, mix and homogenize.

[Example 2] Lotion (1) 1,3-butylene glycol 3.0 (wt%) (2) sorbitol 2.0 (3) ethanol 10.0 (4) carboxyvinyl polymer 1 wt% aqueous solution 10.0 (5) Hypericum perforatum E. 50% ethanol extract 0.5 (6) Methyl paraoxybenzoate 0.1 (7) Perfume 0.1 (8) Purified water 74.3 (6), (7) After dissolving in (3) and adding to (8), (1), (2) and (5) are sequentially added and mixed, then (4) is added, mixed and homogenized.

Example 3 O / W Emulsion Ointment (1) White Vaseline 25.0 (wt%) (2) Stearyl Alcohol 15.0 (3) Sodium Lauryl Sulfate 1.0 (4) Paraoxybenzoic Acid Butyl 0.1 (5) Tomoesou / 98% ethanol extract 0.5 (6) Purified water 58.4 Mix the oil phase components of (1) to (4) and heat to 75 ° C to dissolve and homogenize . The oil phase component is added to (6) heated to 75 ° C to emulsify, and after cooling, (5) is sequentially added, mixed and homogenized at 40 ° C.

[Example 4] O / W emulsion type beauty essence (1) Squalane 5.0 (% by weight) (2) White petrolatum 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0. 8 (5) Polyoxyethylene oleyl ether (20EO) 1.2 (6) Methyl paraoxybenzoate 0.1 (7) Propylene glycol 5.0 (8) Purified water 59.6 (9) Carboxyvinyl polymer 1.0% by weight Aqueous solution 20.0 (10) Potassium hydroxide 0.1 (11) Ethanol 5.0 (12) Hypericum perforatum E. 70% ethanol extract 0.5 (13) Perfume 0.2 Oils of (1) to (6) The phase components are mixed and heated to 75 ° C. to dissolve and homogenize. On the other hand, the water phase components of (7) and (8) are mixed and dissolved to form 7
The mixture is heated to 5 ° C., the above oil phase component is added, and pre-emulsified. After adding (9), emulsify uniformly with a homomixer,
(10) is added to adjust the pH. After cooling at 40 ℃ (11) ~
Add (13), mix, and homogenize.

[Example 5] W / O emulsion type cream (1) Beeswax 3.0 (wt%) (2) Adsorption-purified lanolin 10.0 (3) Squalane 30.0 (4) Solid paraffin 2.0 ( 5) Microcrystalline wax 5.0 (6) Hexyldecyl adipate 10.0 (7) Sorbitan sesquioleate 3.5 (8) Polyoxyethylene hydrogenated castor oil (50EO) 1.0 (9) 1,3- Butylene glycol 5.0 (10) Purified water 29.8 (11) Methyl paraoxybenzoate 0.2 (12) Hypericum perforatum P. water extract 0.5 Mix the oil phase components of (1) to (8) 75 Heat to ℃ to dissolve and homogenize. On the other hand, the aqueous phase components (9) to (11) are mixed and dissolved, heated to 75 ° C., added to the above oil phase components, and uniformly emulsified by a homomixer. After cooling, add (12) at 40 ℃,
Mix.

A usage test was conducted using the above examples.
At that time, those containing no plant extract were used as comparative examples. The panelists selected 20 persons who had pigmentation such as skin spots, freckles, and sunburn as the main symptoms as a group. Each group was allowed to use the examples and comparative examples on the face and hands with a blind, and changes in pigmentation were observed and evaluated. The period of use was 6 months from April to October. About whitening effect, "improved", "slightly improved",
The evaluation was made in three grades of "no change", and the results are shown in Table 2 by the number of panelists who obtained each evaluation.

[0024]

[Table 2] As is clear from the results of the use test shown in Table 2, all of the panelists using the examples in which the extract of the Hypericumaceae plant of a specific type was blended showed improvement in pigmentation. On the other hand, among the panelists using Comparative Examples 1 to 5, no panelists showed a clear improvement, and almost no panelists showed any change. From the above results, it was possible to impart a whitening effect by blending a specific type of Hypericumaceae plant extract.

No panelists complained of skin symptoms such as skin irritation such as pain and itching, or allergic reaction in any of the groups used in the above-mentioned use period. In addition, no deterioration of the emulsified state, no sedimentation and deterioration of the components were observed.

【The invention's effect】

As described above in detail, Hypericum perforatum ( Hyper
The extract of icum erectum Thunb. or Hypericum perforatum L.) and Tomoesou ( Hypericum ascyron L.) has a high inhibitory effect on melanin production. Further, the external agent for whitening skin and whitening skin containing this as a melanin inhibitor Cosmetics exhibit an excellent whitening effect, and also have good safety and stability.

Claims (3)

[Claims] 1. Hypericum erectum Thun
b. or Hypericum perforatum L.), Tomoesou ( Hypericum perforatum L.)
um ascyron L.) 1 type or 2 types or more of the extract is mix | blended, The melanin production inhibitor characterized by the above-mentioned. 2. Hypericum erectum Thun
b. or Hypericum perforatum L.), Tomoesou ( Hypericum perforatum L.)
um ascyron L.) one or more kinds of the extract is blended as a melanin production inhibitor. 3. The whitening agent according to claim 2, wherein the whitening agent is a whitening cosmetic.