KR101959551B1 - A composition for improving skin hypochromatism and protecting the skin from ultraviolet comprising apple mint extracts - Google Patents

Abstract

The present invention relates to a composition for protecting skin against hypoxia and hypochlorite containing apple mint extract as an active ingredient, and a composition comprising an apple mint extract of the present invention as an active ingredient, which comprises Nrf-2 (Nuclear erythroid 2-related factor to promote skin hypogonadism as well as to protect the skin from ultraviolet rays. In addition, the apple mint extract has no cytotoxicity and skin side effects and can be used safely in cosmetic, pharmaceutical and food compositions.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for improving skin hypochromatosis and protecting skin from ultraviolet rays containing an apple mint extract as an active ingredient,

The present invention relates to a composition for protecting skin against ultraviolet rays and improving skin hypochromatosis containing an apple mint extract as an active ingredient. More particularly, the present invention relates to a composition for promoting Nrf-2 (Nuclear erythroid 2-related factor) A pharmaceutical composition and a food composition characterized by comprising an apple mint extract which is capable of improving and preventing hypocholality and protecting the skin from ultraviolet rays as an effective ingredient.

Skin is an organ that acts as a barrier to the external environment and the body, and is always exposed by external or internal stressors. Excessive production of ROS (Reactive Oxygen Species) due to oxidative stress caused by UV, xenobiotics, allergens, and temperature can lead to imbalance of redox state, resulting in lipid, protein (enzyme) , And oxidative damage to DNA, resulting in cell death and deterioration of cellular metabolism (Scharfetter-Kochanek et al ., Biol. Chem. 1997; 378: 1247-1257). It promotes skin aging, inflammatory response and further develops into skin cancer (Wlaschek et al ., J Photoch Photobio B. 2001; 63: 41-51).

Skin is a protective mechanism of oxidative stress and regulates the expression of Nrf-2 (Nuclear erythroid 2-related factor), a cytoplasmic protection mechanism gene, which is mainly present throughout epidermal cells including keratinocytes (Ling et al ., J Immunol. 173: 3467-3481.). Nrf-2 is biosynthesized at a constant level, but its amount is regulated in the cytoplasm by the Nrf-2-Keap1-Cul3 complex. It is attached by Keap1 and degraded by Cul3, resulting in a low level of Nrf-2. However, under oxidative stress conditions, oxidation is induced in the residue of Keap1, which causes Nrf-2 to separate and stabilize in the Nrf-2-Keap1-Cul3 complex and accumulate in the nucleus. .

Nrf-2 is a GST (glutathione S-transferase), NQO1 (quinone reductase NAD (P) H), UTI (UDP-glucuronosyltransferases) and EPHX (epoxide hydrolase), which are phase II detoxifying enzymes with an antioxidant responsive element promotes expression of GCL (c-glutamylcysteine ligase), HO-1 (heme oxygenase-1), GR (glutathione reductase), TrxR (thioredoxin reductase), CAT (catalase), SOD (superoxide dismutase) (Kensler et al , Annu Rev Pharmacol Toxicol 2007; 47: 89-116). This makes it possible to protect skin cells from oxidative stress.

Nrf-2 activation can protect skin cells from UV. Overexpression of Nrf-2 in mice results in increased resistance to apoptosis by UVB irradiation (Kokot et al ., Endocrinology. 2009; 150: 3197-3206). HaCaT, a keratinocyte cell line, is treated with a flavonoid such as quercetin and kaempferol to protect against UV, which increases the amount of Nrf-2, thereby increasing cell viability (Kang et al ., Exp Mol Med 2008; 40: 208-219). SFN (sulforaphane) extracted from broccoli and cabbage increased the expression of antioxidant enzymes NQO1, HO-1 and cGCS by releasing Nrf-2 activated from Nrf-2-Keap1-Cul3 complex to the cytoplasm through modification of Keap1 (Saw et al ., Mol Carcinog. 2011; 50: 479-486). Cancer incidence induced by UV irradiation in mouse cell line SKH-1 was reported to be reduced by SFN (Dickinson et al ., Can Res. 2009; 69: 7103-7110). In addition, in clinical studies, UV irradiation after treatment with SFN was found to reduce erythema by more than 90% (Talalay et al ., Proc Natl Acad Sci USA 2007; 104: 17500-17505).

Recent studies have shown that the Nrf-2-ARE mechanism plays an important role in skin metabolism in the context of oxidative stress. The Nrf-2-ARE mechanism detects oxidative stress and enhances the expression of phase II detoxifying enzymes. In melanocytes, it is known that oxidative stress causes vitiligo. Nrf-2, an oxidative stress defense, plays an important role in the metabolism of melanocytes. People with vitiligo has been reported that the degree of difference between a vitiligo, depending on the activity of Nrf-2 expression was investigated by the lower, Nrf-2 of the (Amin et al, BMC Microbiol 2013 ; 13:.. 158, Jian et al , J Invest Dermatol, 2014; 134 (8): 2221-30). Although the exact cause of such vitiligo is not known, many cases have been reported after physical trauma such as psychological stress, mental shock, sunburn, accident or surgery, pregnancy, internal organs or other diseases. Active oxygen, hydrogen peroxide (H ₂ O ₂ ), is also known to be the cause of vitiligo (Schallreuter et al ., Exp Dermatol. 2008; 17 (2): 139-40). In the case of vitiligo patients, the lack of catalase to remove hydrogen peroxide causes melanin cells to be easily damaged by reactive oxygen species, resulting in immunological attack (Schallreuter et et al. al ., J Invest Dermatol 97: 1081-5, 1991).

On the other hand, apple mint ( Mentha Suaveolens ) is a perennial herb plant, one of the mints belonging to the mint. This plant has been used in a variety of ways, with pain relief and cooling effects, and was used in insect bites and bees. In addition, the effect of clearing the brain was used to alleviate epilepsy symptoms. Apple mint leaf promotes digestion, so when you have a stomachache or intestinal problems, take it by car. It is also known to have anti-cancer effects. Leaves contain various ions, potassium, calcium, vitamins A and C. However, until now, there has been no research on whether apple mint activates the Nrf-2-ARE mechanism.

Thus, the present inventors have observed the activity in the Nrf-2-ARE mechanism for apple mint and found that apple mint can promote Nrf-2 activation, thus completing the present invention.

Accordingly, the technical problem to be solved by the present invention is to provide a skin-protecting agent which not only protects skin from ultraviolet rays but also has excellent human safety by promoting activation of Nrf-2 (Nuclear erythroid 2-related factor) .

In order to solve the above technical problems, the present invention provides a composition for improving skin hypochromatosis or protecting skin from ultraviolet rays, which comprises an apple mint extract as an active ingredient.

Preferably, the composition comprising the apple mint extract in the present invention may be a cosmetic composition, a pharmaceutical composition or a food composition.

The term " extract " of the present invention refers to a preparation which is obtained by squeezing a herbal medicine with an appropriate leaching solution and concentrating the liquid by evaporating the leaching solution. The extract solution obtained by the extraction treatment, diluted solution of the extract solution, Dried products obtained by these methods, their controlled products or purified products.

The apple mint extract of the present invention can be produced using common extraction, isolation and purification methods known in the art. Examples of the extraction method include, but are not limited to, hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction.

According to a specific embodiment of the present invention, the apple mint extract may be prepared by drying apple mint, pulverizing it, extracting it with water, an organic solvent or a mixture thereof, and then filtering it under reduced pressure. In this case, examples of the organic solvent include methanol, ethanol, propanol or butanol, preferably ethanol, more preferably 70% ethanol.

In the composition of the present invention, the apple mint extract may contain 0.00001 to 50 wt%, more preferably 0.0001 to 30 wt%, and most preferably 0.0001 to 10 wt%, based on the total weight of the composition. When the content of the apple mint extract is less than 0.00001% by weight, the effect of improving the skin's low tone and protecting the skin from ultraviolet rays is weak. When the content of the apple mint extract is more than 50% by weight, There is a problem that the stability of the shape can not be secured.

According to one embodiment of the present invention, there is provided a composition for improving skin hypochromatosis comprising an apple mint extract as an active ingredient. The composition of the present invention protects skin cells from ultraviolet rays while having little cytotoxicity. In particular, it activates melanocytes that protect skin from ultraviolet rays to exert skin protection effect. (Skin soothing effect, skin blackening inducing effect, skin cancer prevention effect).

As used herein, the term " hypochromogenesis " means an action to improve skin troubles such as vitiligo and white moth caused by abnormal melanin cell or melanin pigment synthesis function and reduced melanin production.

In the composition for improving skin hypochromatosis according to the present invention, the apple mint extract increases the melanin production of human-derived melanocytes, has high stability, and has little side effects such as skin irritation induction.

According to one specific embodiment of the present invention, the apple mint extract can improve skin hypochloremia by promoting Nrf-2 activation, and not only inhibits the cell death of keratinocytes induced by ultraviolet rays, It has excellent preventive effect.

According to one preferred embodiment of the present invention, the present invention provides a cosmetic composition comprising an apple mint extract as an active ingredient.

The cosmetic composition according to the present invention may contain, in addition to the apple mint extract as an active ingredient, conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, can do.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a powder or a spray, tosse, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tragacanth.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

According to one preferred embodiment of the present invention, the present invention provides a pharmaceutical composition comprising an apple mint extract as an active ingredient.

The pharmaceutical composition according to the present invention includes a pharmaceutically acceptable carrier in addition to the apple mint extract. The pharmaceutically acceptable carrier to be contained in the pharmaceutical composition of the present invention is one usually used at the time of formulation, and includes lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably applied by parenteral administration, more preferably topical application by application.

A suitable dosage of the pharmaceutical composition of the present invention may vary depending on such factors as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, . The dosage of the pharmaceutical composition of the present invention is in the range of 0.001-100 mg / kg on an adult basis. When the composition is an external preparation, it is preferably applied in an amount of 1.0 to 3.0 ml on an adult basis once to five times a day, and continued for 1 month or more. However, the dose is not intended to limit the scope of the present invention.

The pharmaceutical composition of the present invention may be prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art, Into the container. The formulations may be in the form of solutions, suspensions, syrups or emulsions in an oil or aqueous medium, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

According to one preferred embodiment of the present invention, the present invention provides a food composition comprising an apple mint extract as an active ingredient.

The food composition according to the present invention may further contain not only an apple mint extract as an active ingredient but also ingredients normally added in food production such as proteins, carbohydrates, fats, nutrients, seasonings and flavors.

Examples of such carbohydrates are monosaccharides, such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, .

On the other hand, the apple mint extract of the present invention is a natural substance which is harmless to the human body and has little toxicity and side effects, so that it can be safely used for a long period of use and can be safely applied to cosmetic, pharmaceutical and food compositions as described above .

As described above, the composition comprising the apple mint extract of the present invention as an active ingredient not only improves skin hypochromatosis by promoting Nrf-2 (Nuclear erythroid 2-related factor) activation, but also has an effect of protecting skin from ultraviolet rays . In addition, the apple mint extract has no cytotoxicity and skin side effects and can be used safely in cosmetic, pharmaceutical and food compositions.

1 is a graph showing the effect of promoting Nrf-2 activity of apple mint extract in keratinocyte.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1: Preparation of apple mint extract

The ground portion of apple mint was taken and cleaned thoroughly to remove impurities and impurities. 2 L of purified water was added to 40 g of the raw material, and the mixture was subjected to hot water extraction in a sterilizer. The supernatant was concentrated using a concentrator and then lyophilized to give a powder. The powdered extract was dissolved in sterilized distilled water at a concentration of 100 mg / mL, and then passed through a water syringe filter of 0.2 μm pore size and used in the experiment.

<Example 2> Nrf-2 activity test

In order to measure Nrf-2 transcription factor activity, an ARE-LUC vector (Promega, Madison, WI, USA) containing a luciferase gene was inserted into the ARE (Antioxidant response elements) of the promoter to which Nrf- (Human Keratinocyte) HaCaT cell line was used. HaCaT cells were inoculated with 35 X 10 ⁴ cells in a 60-mm culture dish containing DMEM medium containing 10% FBS, 1% penicillin-streptomycin and 200 μg / ml hygromycin-B, CO ₂ and 37 ° C for 48 hours. After 48 hours, the apple mint extract of Example 1 diluted to 50 μg / ml in DMEM medium containing 1% FBS was treated and cultured for 24 hours.

The experiments were carried out according to the method described in the commercially available Luciferase assay kit (Promega, E1500). After 24 hours of treatment with apple mint extract, the medium was removed, washed with PBS, and cells were lysed using a cell culture eluant (Promega, E153A). The cell lysate was centrifuged at 12,000 g at 4 ° C for 2 minutes, and the supernatant was used.

20 μl of the obtained supernatant was reacted with 100 μl of luciferase assay substrate and fluorescence intensity was measured using a microplate reader (Infinite f200, Tecan). HaCaT cells treated with 3 uM t-butylhydroquinone (tBHQ) were used as the positive control group and HaCaT cells treated with D.W were used as the negative control group. The results are shown in Fig.

1 is a graph showing the effect of promoting Nrf-2 activity of apple mint extract in keratinocyte. As shown here, the experimental group treated with 50 μg / ml apple mint extract showed a 4-fold increase in Nrf-2 activity compared to the negative control group.

<Example 3> UV protection effect of apple mint extract

Cell viability tests were conducted to evaluate whether apple mint extracts could prevent UV induced cytotoxicity by activating antioxidant enzymes in the skin.

The keratinocytes were cultured in medium consisting of penicillin-streptomycin and serum in DMEM base medium. The cells were inoculated into a 12-well plate at 1 × 10 ⁵ cells per well and cultured at 37 ° C. under 5% CO ₂ until cells were attached to the bottom of the wells at about 80% or more. Apple mint extract was treated by concentration. After 24 hours of treatment, the culture medium was changed to PBS, and ultraviolet rays (UVB 20 mJ) were irradiated. After UV irradiation, the medium was replaced with DMEM medium containing apple mint extract, and the cell viability was further measured by MTT assay at the end of culture for 24 hours. The cytoprotective effect of the apple mint extract is shown in Table 1 below.

sample Treatment concentration  (%) Untreated group reference cell viability Untreated group - 100 UVB 20 mJ 54 UVB + Apple Mint Extract 10 ppm 52 50 ppm 67 100 ppm 77

As shown in Table 1, the apple mint extract showed an effect of suppressing the cell death of keratinocytes induced by ultraviolet rays in a concentration-dependent manner.

< Example  4> Effect of apple mint extract on prevention of vitiligo

In this experiment, we examined melanin test to determine whether apple mint extract inhibits H ₂ O ₂ - induced decrease in melanin production in melanocytes. Human epidermal melanocytes were inoculated into 6-well plates at 2 × 10 ⁵ per well and cultured at 37 ° C in 5% CO ₂ until the cells were attached to the wells at least 80%. After culturing, ₂₀₀ μM H ₂ O ₂ and apple mint extract were added to the culture medium at a concentration of 5% CO ₂ at 37 ° C. for 5 days. On the last day of culture, the medium was removed, washed with PBS (phosphatized buffer saline), and treated with trypsin to recover the cells. The recovered cells were counted using a hematocytometer, and the number of cells in each treatment group was adjusted to the same number, and the cells were divided into 2-mL tubes, centrifuged at 5,000 to 10,000 rpm for 10 minutes, and then the supernatant was removed The pellet was obtained. The cell pellet was dried at 60 ° C., and 100 μl of a 1 M sodium hydroxide solution containing 10% DMSO was added thereto to obtain intracellular melanin in a 60 ° C. thermostatic chamber.

Then, the absorbance at 490 nm was measured with a microplate reader, and the amount of melanin per cell constant was determined, and converted into a percentage. The results are shown in Table 2 below.

sample Treatment concentration  (%) Untreated group Melanin level Untreated group - 100 H ₂ O ₂ 200 uM 63 H ₂ O ₂ + apple mint extract 10 ppm 69 50 ppm 74 100 ppm 82

As shown in Table 2, it can be confirmed that the apple mint extract increases the amount of melanin produced by H ₂ O ₂ in a concentration-dependent manner.

<Example 5> Test for confirming safety of human mint extract against human skin

To verify that the apple mint extract is safe for human skin, a skin safety verification experiment was conducted. For this purpose, cumulative skin irritation test was performed. The formulation containing 0.1%, 0.5% and 1% of apple mint extracts was prepared and used in a healthy 30 adults for 9 times every 24 hours in the upper forearm area to obtain apple mint extract And whether the skin was stimulated or not was measured. A pin chamber (Finn chamber, Epitest Ltd, Finland) was used as the deposition method. 15 [mu] l of each of the above external preparations for skin was dropped into the chamber, followed by applying a patch. The degree of reaction on the skin each time was scored using the following Equation 1, and the results are shown in Table 3 below.

A score of ±, a +, and a ++ is given as 1, 2, and 4, respectively, and a safe composition is determined when the average degree of reaction is less than 3.

Test substance Number of subjects who responded Average
Reactivity 1 week 2 weeks 3 weeks Primary Secondary Third Fourth 5th 6th Seventh 8th car 9th ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ Control group
(Squalene) 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Apple mint
extract
(0.1%)
[Test group 1] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Apple mint
extract
(0.5%) [Test group 2] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Apple mint extract
(One%)
[Test group 3] 1 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.09

In Table 3, the numbers of persons corresponding to ±, + and ++ in all of test groups 1, 2 and 3 were 0, and the average reactivity was also 0.00. Therefore, the apple mint extract was judged to be a skin-safe substance which does not exhibit a distinct cumulative stimulation pattern, since the average degree of reactivity is 3 or less.

The composition of the present invention can be prepared in the form of a formulation as described below, but is not limited thereto.

Formulation Example 1: Cosmetic preparation

1-1. Softening longevity

ingredient weight% Apple mint extract 0.01 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 ethanol 10.0 Triethanolamine 0.1 Preservatives, micronutrients, trace fragrances and trace water 82.79 sum 100.0

1-2. Nutritional lotion

ingredient weight% Apple mint extract 0.01 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water 69.69 sum 100.0

1-3. Nourishing cream

ingredient weight% Apple mint extract 0.01 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water 56.79 sum 100.0

1-4. Massage cream

ingredient weight% Apple mint extract 0.01 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water 27.49 sum 100.0

1-5. pack

ingredient weight% Apple mint extract 0.01 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonyl phenyl ether 0.3 Preservatives, micronutrients, trace fragrances and trace water 81.39 sum 100.0

Formulation Example 2: Pharmaceutical preparation

2-1. Manufacture of Powder

ingredient Content (g) Apple mint extract 2 Lactose One

The above components were mixed and packed in airtight bags to prepare powders.

2-2. Manufacture of tablets

ingredient Content (mg) Apple mint extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

2-3. Preparation of capsules

ingredient Content (mg) Apple mint extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

As described above, the composition comprising the apple mint extract of the present invention as an active ingredient not only improves skin hypochromatosis by promoting Nrf-2 (Nuclear erythroid 2-related factor) activation, but also has an effect of protecting skin from ultraviolet rays . In addition, the apple mint extract has no cytotoxicity and skin side effects and can be used safely in cosmetic, pharmaceutical and food compositions.

Claims (7)

A cosmetic composition for improving or preventing hypocholesterolemia, which comprises an apple mint extract as an active ingredient. A pharmaceutical composition for treating or preventing skin hypochlorite characterized by comprising an apple mint extract as an active ingredient. A food composition for improving or preventing skin hypochlorite characterized by containing an apple mint extract as an active ingredient. The method according to claim 1,
Wherein the improvement or prevention of skin hypochlorite is improved or prevented by promoting activation of Nrf-2 (Nuclear erythroid 2-related factor). The method according to claim 1,
Wherein the skin hypochromatosis is vitiligo or white moth. The method according to claim 1,
Wherein the apple mint extract is prepared by drying apple mint, pulverizing it, extracting it with water, an organic solvent or a mixture thereof, and then filtering it under reduced pressure. The method according to claim 1,
Wherein the apple mint extract is contained in an amount of 0.00001 to 50% by weight based on the total weight of the composition.

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