KR20120044396A - Composition for improving atopic dermatitis comprising extract of quercus variabilis which is extracted from wood chip of quercus variabilis obtained by steam explosion treatment - Google Patents

Abstract

PURPOSE: A composition containing Quercus variabilis extract is provided to treat atopic dermatitis without toxicity. CONSTITUTION: A composition for treating atopic dermatitis contains Quercus variabilis extract as an active ingredient, prepared using solvent. Quercus variabilis is treated at 20-30 kg/cm for 2-15 minutes. The composition is a cosmetic, pharmaceutical, or functional food composition. The cosmetic composition is manufactured in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, or spray.

Description

Composition for improving atopic dermatitis comprising extract of Quercus variabilis which is extracted from wood chip of Quercus variabilis obtained by steam explosion treatment }

The present invention relates to a composition for improving atopic dermatitis, which comprises an oyster oak extract extracted from a fired oyster oak wood chip as a solvent.

Atopic dermatitis is a type of hypersensitivity that has recently become increasingly dangerous as the prevalence increases rapidly. From an immunological point of view, atopy is a major symptom due to the immune response of immunoglobulin E and allergin. It is defined as a group of allergic diseases with a strong inherited tendency.

In particular, atopic dermatitis, a typical symptom of atopic dermatitis, is estimated to be related to genetic factors and immune system deficiency, although 0.5% -1% of the population and 5-10% of children complain of severe symptoms. The exact cause has not been determined yet, but it is expected to be somewhat alleviated by improving the environment and diet, and there is no fundamental treatment.

However, atopic dermatitis, although showing individual differences, mostly involves severe itching, including dry skin, rashes, rashes, scabs, scaly skin (human scales), and emotional anxiety, stress, tension, and frustration. In addition to feelings of anger and other allergic diseases such as hives, metal allergies, asthma and allergic rhinitis, it is a serious social problem that threatens national health today.

Meanwhile, representative drugs known to be effective in atopic dermatitis to date include steroids, antihistamines, antibiotics, nonsteroidal and other sedatives and neuro stabilizers.

Of these, steroids have excellent effects on anti-inflammatory and immunosuppressive effects, but when used for long periods of time, the elasticity of the skin disappears and becomes thinner, resulting in unpleasant bleeding. In severe cases, hormonal systemic symptoms, adrenal function and immunity decrease. Appears, there are disadvantages that the symptoms explode worse when you stop the drug.

In addition, antihistamines are only temporary measures to alleviate the symptoms of itching by preventing histamine from being released from mast cells, and when used for a long time, side effects such as insomnia and anorexia appear.

In addition, antibiotics are intended to prevent secondary bacterial infections caused by scratching, rather than being a fundamental therapeutic drug for atopic dermatitis.In addition to increased resistance and decreased gastrointestinal function with prolonged use, hepatic damage and gastric colitis, Can cause aplastic anemia due to bone marrow function.

In addition, other nonsteroidal drugs are only moisturizers for the relief of symptoms of atopic dermatitis, and sedatives and neurostabilizers are only supplements for symptomatic relief, rather than fundamental treatment.

Moreover, these existing atopy therapies differ depending on the individual's constitution, and premise of the addition and use of artificial chemical compositions, especially during the composition and manufacturing process, and therefore, unlike the pharmacological components, they may act as serious allergens in atopic patients. This is very large and it is difficult to trust its safety.

Accordingly, the present inventors completed the present invention by confirming that while searching for a natural product useful as a therapeutic agent for atopic dermatitis, the oyster oak extract extracted from the crushed oyster oak wood chips as a solvent has excellent antioxidant activity and shows an improvement effect on atopic dermatitis. It was.

Accordingly, an object of the present invention is to provide a composition for improving atopic dermatitis, which contains an oyster oak extract extracted as a solvent from an exploded oyster oak wood chip that can exhibit an improvement effect on atopic dermatitis.

In addition, another object of the present invention is a cosmetic composition, pharmaceutical composition and functional for improving atopic dermatitis containing oyster oak extract extracted as a solvent an explosive treated oyster oak wood chip that can exhibit an improvement effect on atopic dermatitis It is to provide a food composition.

In order to achieve the above object, the present invention provides a composition for improving atopic dermatitis, which contains the oyster oak extract extracted from the oyster oak wood chip treated with a solvent as an active ingredient.

The explosive treatment may be steamed for 2 to 15 minutes at a pressure of 20 to 30 kg / cm, preferably steamed for 10 minutes at a pressure of 25 kg / cm. If the process is blasted out of the above conditions may cause problems such as carbonization of oyster oak sample and high temperature of the extract, change of the active ingredient by high pressure.

The oyster oak extract may be a hot water extract or an aqueous ethanol extract.

The oyster oak hot water extract is a step of adding distilled water to the crushed oak oak wood chips; Extracting hot water; Filtration; Concentrating; And it can be obtained through the step of lyophilization, the hot water extraction step may be hot water extraction for 30 to 120 minutes at 90 to 130 ℃, preferably at 121 ℃ for 30 minutes.

In addition, the oyster oak ethanol extract is a step of adding and extracting ethanol or an ethanol aqueous solution to the chopped oak wood chips; Filtration; Concentrating; And lyophilization, wherein the aqueous solution of ethanol uses 50 to 95% aqueous ethanol solution, and the extraction step is performed for 8 to 7 days at room temperature or boiling temperature, preferably at 25 ° C. for 24 hours. Can be extracted during.

The oyster oak extract may be extracted by adding 10 to 50 parts by weight of a solvent based on 1 part by weight of oyster oak. The solvent may be water, ethanol or an aqueous ethanol solution.

The composition according to the present invention may include 0.1 to 99.9% by weight of the oyster oak extract extracted with a fired oyster oak wood chips as a solvent. If included outside the content range, the atopic dermatitis improving effect may be insignificant or may cause other side effects.

The composition of the present invention is hyaluronic acid, cetearyl alcohol, beeswax, cetearyl glucoside, jojoba seed oil, apricot seed oil, burdock extract, lemon extract, hops extract, red pepper extract, sage leaf extract, calendula flower extract , Soap grass extract, Cinnabar fruit extract, Pasqueflower extract, Essonia extract, Disodium adenosine triphosphate, Algin, Papaya fruit extract, Portulaca extract, Polyacrylate-13, Polyisobutene, Polysorbate, Sodium polyacrylate , Ethylhexyl stearate, trideces-6, disodium id, potassium hydroxide, glyceryl stearate, fiji-100 stearate, squalane, dimethicone, niacinamide, tocopheryl acetate and dipotassium glyc It may further comprise any one or two or more selected from the group consisting of a series.

In addition, the composition of the present invention may be provided in various forms selected from cosmetic compositions, pharmaceutical compositions or functional food compositions.

When the composition of the present invention is a cosmetic composition, the formulation of the cosmetic composition may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, Powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like can be formulated as, but are not limited to. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

Preferably, the cosmetic composition may be in the form of a cream containing 0.1 to 10.0 parts by weight of the oyster oak extract extracted with a solvent crushed oak wood chips with respect to 100 parts by weight of a moisturizing agent.

When the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier components. Can be.

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and in the case of a spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizer or an emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, as a carrier component Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the cosmetic composition is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid. Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the composition of the present invention is a pharmaceutical composition, the pharmaceutical composition may include a pharmaceutically acceptable carrier in addition to the oyster oak extract, such a pharmaceutically acceptable carrier is commonly used in pharmaceutical preparations. , Lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methyl Hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like, but are not limited thereto. In addition, the pharmaceutical composition may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like as an additive.

The pharmaceutical composition is determined by the method of administration depending on the symptoms of atopic dermatitis, usually topical administration is preferred. In addition, the dosage of the active ingredient in the pharmaceutical composition may vary depending on the route of administration, the degree of the disease, the age, sex, and weight of the patient, and may be administered once to several times daily.

The pharmaceutical compositions may be prepared in unit dose form or formulated using pharmaceutically acceptable carriers and / or excipients or may be prepared within a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion, or may be in the form of an exercicide, extract, powder, granule, tablet, warning, lotion, ointment, or the like.

In addition, when the composition of the present invention is a functional food composition, the functional food composition may be prepared from gums, vitamin complexes, dietary supplements, special nutritional supplements, functional drinks and the like.

In addition to the green tea leaf extract as an active ingredient, the functional food composition of the present invention, glucose, monosaccharides such as fructose, disaccharides such as maltose, sucrose, or polysaccharides such as dextrin, cyclodextrin may be added, Sugar alcohols such as xylitol, sorbitol, erythritol and the like may also be added, and sweeteners such as taurine, stevia extract and the like may also be added.

When the composition of the present invention is added to food for the purpose of improving atopic dermatitis, it may generally be added at 1 to 20% by weight of the total food weight, and when added to a beverage, 0.1 to 100 mL based on 100 mL , Preferably in an amount of 10 to 100 mL.

On the other hand, oyster oak extract used in the composition of the present invention as a natural product, there is little toxicity and side effects can be used with confidence even for long-term use for the purpose of prevention.

According to the present invention, the composition containing the oyster oak extract extracted from the crushed oak oak wood chips as a solvent shows an effect of improving atopic dermatitis through clinical tests and histamine test, and only active ingredients without using chemicals Since the composition for improving atopic dermatitis, it can be usefully used in the development of cosmetics, drugs and foods for improving atopic dermatitis.

1 is a result of measuring the main polyphenol content of the oyster oak extract according to the present invention using HPLC,
Figure 2 is the result of measuring the antioxidant activity against DPPH of the oyster oak extract according to the present invention,
Figure 3 shows the age distribution of the subjects of atopic patients selected to examine the atopic dermatitis treatment effect of oyster oak extract according to the present invention,
Figure 4 shows the therapeutic effect of atopic dermatitis of oyster oak extract according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited by these examples.

Example 1 Preparation of Oyster Oak Extract

Extracted materials were steamed wood chips ( Quercus variabilis ) for 10 minutes at a pressure of 25 kg / cm, and 20 g of wood chips were used in 500 mL of solvent for hot water extraction and ethanol extraction, respectively. At this time, the hot water extraction was extracted for 30 minutes at 121 ℃ and ethanol extraction for 24 hours in a 25 ℃ shake incubator. Each extract was centrifuged at 3000 rpm for 30 minutes and the supernatant was separated, filtered through a 0.45 μm membrane filter (Sartorius ^® , Germany) and used as an HPLC sample. At this time, oyster oak wood chips were not prepared for comparison.

Example 2 Analysis of Main Polyphenol Content by HPLC

HPLC (Gilson ^® , France) analysis was performed to analyze the main polyphenols contained in each extract. Calibration curves of vanillic acid, ferulic acid, vanillin and cinnamic acid, which are the main polyphenols, were prepared and the retention time was confirmed. The analysis sample of each extract was used 20µl and the analyzed extract was quantified the main polyphenol content by using the calibration curve. At this time, the experiment was repeated three times to use the average value, the formula for quantifying the content of the main polyphenol is as shown in the following equation (1).

[Equation 1]

The main polyphenol content of unexploded oak oak wood chips was measured using HPLC, and no major polyphenols were detected in all extracts. On the other hand, the crushed oak oak wood chip extract showed the main polyphenols (vanillic acid, ferulic acid, vanillin, cinnamic acid) content as shown in Table 1 and FIG.

Extraction yield (%) Phenolic Acid Content, mg / g ^* Vanillic acid Ferulic acid vanillin Sinnamsan total Hydrothermal extract 17.23 3.60c 1.10c 1.10a - 5.80 25% Ethanol Extract 20.94 3.20c 1.20c 0.70a - 5.10 50% Ethanol Extract 28.75 17.30a 1.90b 1.40a - 20.60 75% Ethanol Extract 24.55 10.80b 1.20c 1.10a - 13.10 95% Ethanol Extract 15.33 10.90b 2.80a 0.80a - 14.50

The yields of hot water extract and ethanol extract were similar. Overall, the main polyphenol contents of oyster oak were high in 50%, 75% and 95% ethanol extracts and relatively low in hot water extract and 25% ethanol extract. Oyster oak extract showed higher content of vanillic acid than other major polyphenols. The content of vanillic acid was the highest at 17.30mg / g in 50% ethanol extract, which was statistically significant with other extraction conditions. The content of ferulic acid was most detected as 2.80mg / g in 95% ethanol extract, and statistically significant with other extraction conditions. The content of vanillin was the highest at 1.40mg / g in 50% ethanol extract, which was statistically significant with other extraction conditions. Cinnamic acid, on the other hand, was not detected in all extracts.

Example 3 Analysis of Volatile Compound Content Using GC-MS

Volatile compounds of wood vinegar and oyster oak wood chips (steamed at 25 kg / cm ² pressure for 10 minutes) were analyzed by GC-MS, and the identification of the compounds was carried out using MS library data. It is based on a compound already known as an extract component of wood. The extracts of wood are very diverse in their components, especially in the presence of large amounts of isomers, making it almost impossible to identify all of these compounds. Therefore, most of them are identified by comparing MS data with commercial products. In this experiment, it was identified using commercially-owned products and MS data. However, in the case of using MS data, confirmation using commercially available products is essential for reliable identification.

1. Volatile Compounds in Wood Vinegar

Volatile compounds of wood vinegar were 29 kinds, and total 79.98% of volatile compounds were measured (Table 2). The main volatile compounds of wood vinegar include phenol (21.71%), ρ-cresol (9.38%), 4-ethylphenol (6.96%), m-cresol (6.04%) and 4-ethylguaiacol (5.13%). Trace and other compounds were identified (Moon, Sung-Pil, Chang-Seop Koo. Volatile Components in Bamboo and Wood Vinegar by SPME Method, Wood Engineering, 2002; 30 (4): 80-86).

Peak NO. RT (min) compound % One 3.9 Acetone 0.50 2 5.3 2-butanone 3.19 3 14.1 Allyl alcohol 0.43 4 17.7 Cyclopentanone 0.51 5 25.9 1-hydroxy-2-propanone 1.24 6 36.8 Acetic acid 8.70 7 41.3 2,3-dimethyl-2-cyclopenten-l-one 1.15 8 44.1 5-methyl-2-furoate 1.80 9 46.5 Hexahydro-3-methylene-2 (3H) -benzofuranone 0.43 10 59.7 3-methyl-1,2-cyclopentanedione 0.35 11 61.6 Guayakol 4.98 12 62.2 4-methyl guayacol 0.56 13 64.6 2,6-dimethyl phenol 1.21 14 69.4 1-indanon 0.60 15 69.5 Trimethyl phenols 0.53 16 70.0 phenol 27.14 17 71.0 4-ethyl guayacol 6.41 18 73.5 2-ethyl phenol 1.21 19 73.8 2,5-xylenol 1.94 20 74.0 ρ-cresol 11.85 21 74.4 m-cresol 7.55 22 75.1 4-propyl guayacol 1.18 23 77.0 Ethylmethyl phenols 0.79 24 77.4 Dimethyl phenols 0.75 25 78.7 4-ethyl phenol 10.47 26 79.1 3-ethyl phenol 1.28 27 80.9 Dimethyl phenols 1.31 28 81.9 Siringol 1.44 29 83.1 4-propyl phenol 0.51 Total 100

2. Volatile Compounds of Extracted Oyster Oak Chips

Forty-five volatile compounds of the crushed oak wood chip extract were measured. The main volatile compounds of the explosive oyster oak wood chip extracts were 4-ethyl siringol (10.11%), 4-methyl guayacol (9.45%), 4-methyl shiringol (8.12%), and shiringol (6.88%). ), 4-propyl guayacol (6.86%), guayacol (5.87%), 4-propyl syringol (5.03%), and other compounds were identified in trace amounts.

Peak No. RT (min) compound % One 20.13   Acetic acid 2.67 2 20.69   Furfural 0.90 3 22.95   Tetrahydrofurfuryl alcohol 0.88 4 24.35   2,3-dimethyl cyclopenten-l-one 1.31 5 26.50   5-methyl-2-furfural 0.83 6 28.77   - 0.81 7 30.64   - 0.70 8 39.68   3,4-dimethoxy toluene 1.10 9 42.62   Guayakol 5.87 10 43.19   3 (or 6) -methyl guayacol 1.03 11 47.04   6 (or 3) -methyl guayacol 0.81 12 47.60   cis-5-butyldihydro-4-methyl-2 (3H) -furanone 1.82 13 47.85   4-methyl guayacol 9.45 14 48.17   Trimethyl phenols 0.71 15 48.38   3 (or 6) -ethyl guayacol 1.05 16 50.32   1-indanon 1.48 17 50.60 o -cresol 1.97 18 50.67   phenol 1.67 19 50.98   - 0.73 20 51.97   Phenols 1.27 21 52.84   3-ethyl siringol 1.87 22 53.05   Aceto Guayacon 0.66 23 53.43   Octanoic acid 1.16 24 54.17   2-ethyl phenol 0.73 25 54.42   2,5 (or 4) -xyleneol 1.72 26 54.64   2,4 (or 5) -xyleneol 2.74 27 55.04 p- cresol + m -cresol 2.66 28 55.67   Acetoshiringon 1.02 29 55.85   4-propyl guayacol 6.86 30 58.02   2,3-xylenol 0.77 31 58.64   2-phenyl-2-butenal 0.64 32 58.79   Eugenol 1.24 33 58.91   Ethylmethyl phenols 1.41 34 59.09   Ethylmethyl phenols 0.73 35 59.34   3,5-xylenol 2.87 36 59.70   3-ethyl phenol 0.78 37 61.01   Trimethyl phenols 0.83 38 61.58   3,4-xylenol 1.29 39 61.74   Trimethyl phenols 1.10 40 63.16   Trans-isoyugenol 0.98 41 63.53   Ethylmethyl phenols 0.74 42 63.68   Siringol 6.88 43 67.74   4-methyl siringol 8.12 44 70.43   4-ethyl siringol 10.11 45 73.58   4-profile shiringol 5.03 Total 100

In each of the two volatile compounds, acetic acid, 2,3-dimethyl cyclopenten-l-one, guayacol, trimethyl phenols, 1-indanone, phenol, 2-ethyl phenol, 2,5 (or 4) -xyl Knoll, p-cresol + m-cresol, ethyl methyl phenols, trimethyl phenols, cylingol, 3-ethyl phenol, trimethyl phenols, etc., were commonly included, and phenol (27.14%) and cresol, which are harmful substances, were contained in wood vinegar. (19.40%) contained a large amount of about 50%, and the crushed oak wood chip extract contained about 7% of phenol (3.04%) and cresol (3.64%), which are harmful substances. It contained. This means that wood liquor should not be used directly on the lesion and must be purified to remove impurities.

Example 4 Total Polyphenol Content Analysis (Folin-Ciocalteu Method)

In order to measure the total polyphenol content, dry powders of hot water extracts of oyster oak, 95%, 75%, 50% and 25% ethanol extracts were used, and Folins-Ciocalteu method (Acta Derm Venerol (Stockh) (1980); 92 (Suppl): 44-47). That is, 30 μl of the sample solution was mixed with 3 ml of distilled water, and then 100 μl of 2N Folin-Ciocalteu's phenol reagent (SIGMA, USA) was added thereto, left for 5-8 minutes, and then mixed with 300 μl of 20% sodium carbonate. After reacting at room temperature for 30 minutes, its absorbance was measured by a UV-spectroscopy analyzer (U-3000 ^® , Hitachi, Japan). The total polyphenol content of the sample was obtained by comparing the calibration curve prepared with gallinic acid (mg / g). At this time, the experiment was repeated three times to use the average value.

 Polyphenol contents according to the extraction conditions of the oyster oak extract are shown in Table 4. The polyphenol contents of 25.645m / g and 26.154mg / g were high in 25% and 50% ethanol oak extract, respectively, and the lowest polyphenol content was 14.801mg / g in hot water extract. All ethanol extracts and hot water extracts showed statistically significant results.

Test  Total Phenolic Content (mg / g Aerated Wood Chips) Hydrothermal extract  14.801 ± 5.767b 25% Ethanol Extract  25.645 ± 8.962a 50% Ethanol Extract  26.154 ± 0.756a 75% Ethanol Extract  18.426 ± 0.440ab 95% Ethanol Extract  19.441 ± 1.050ab

Example 5 Antioxidant Activity Assay (DPPH Radical Scavenging Activity Review)

Antioxidant activity of oyster oak extract was modified by Wang et al. (Sumor 1979; 17: 105-110) and the reducing power of each sample was measured by the electron donating effect of each extract. That is, dry water extract powder of oyster oak, 95%, 75%, 50% and 25% ethanol extract dry powder was used, BHT (Butylated Hydroxy Toluene, Sigma, USA), DPPH (2,2-diphenyl-1 -picryl-hydrazyl, Sigma, USA) and 100% ethanol were used.

In order to measure the antioxidant activity against DPPH, a DPPH sample was dissolved in ethanol to prepare 1.5 × 10 ^-4 M DPPH solution. A sample solution and a BHT solution were prepared by preparing powders and BHT in the same concentration in ethanol. The sample solution and the BHT solution were used as test strips and ethanol was used as a control strip. 50 μl of each sample solution was added to 200 μl of DPPH to give a final volume of 250 μl. After blocking the light for 30 minutes, the antioxidant activity was calculated by measuring the absorbance at 517 nm using an ELISA (Anthos ^® , Austria) instrument. At this time, the experiment was repeated three times to use the average value.

Experimental results for the antioxidant activity of the oyster oak extract by DPPH is shown in FIG. The 75% ethanol extract of oyster oak showed the highest antioxidant activity (82.4%), and the antioxidant activity of the synthetic antioxidant BHT was higher than 50.6%, which was statistically significant. On the other hand, hot water extract showed the lowest antioxidant activity (5.6%), and ethanol extraction was more suitable for extracting oyster oak than hot water extract.

Example 6 Review of Atopy Treatment Efficacy

1. Preparation of Atopic Treatment Formulations

In order to see the effect on atopic dermatitis, the preparation containing the 1% of each of the hydrolyzed oak hot water extract and 95%, 50% and 25% ethanol extract prepared in the examples prior to the commercial moisturizer (MD7 ^® , Kolmar, Korea) were prepared. Twenty patients with atopic dermatitis and other dermatitis patients (psoriasis patients, molecular eczema patients) were subjected to a pilot test. This oak extract formulation had an improvement effect only in patients with atopic dermatitis. Itching improved but had no effect on inflammation. Since there was no difference in the symptom improvement effect according to the formulation, it was decided to evaluate the efficacy on atopic dermatitis using 95% ethanol extract, which is easy to prepare.

2. Clinical efficacy evaluation

The patients who were diagnosed with atopic dermatitis according to the Korean Society of Dermatology Atopic Dermatitis criteria were visited from April 2008 to June 2008. Age distribution of atopic patients by age is shown in FIG. 3. A total of 21 patients with atopic dermatitis were included. There were 10 males and 11 females with an average age of 12.86 ± 8.47 years. The age distribution was 10 (47.62%) for 10 years or younger, 9 (42.86%) for 10 to 20 year olds, and 2 for 20 or older and 90% or more of the total number of patients.

The symptoms and itching of atopic dermatitis to determine the therapeutic effect of the atopic dermatitis treatment agent mixed with oyster oak extract were divided into three grades 1: mild, 2: moderate, 3: severe, and the same dermatologist questioned and recorded. The criteria for treatment results were divided into 5 grades: 0: no effect or worsening, 1: 20% or less, 2: 21-40%, 3: 41-60%, 4: 61-80%, 5 : Measured and recorded over 80% improvement.

Figure 4 shows the improvement of using a control moisturizer MD7 used as a treatment for atopic patients and MD7 to which the oyster oak extract as a test was added to men and women. The criteria for treatment results were divided into 5 grades: 0: no effect or worsening, 1: 20% or less, 2: 21-40%, 3: 41-60%, 4: 61-80%, 5 : Measured and recorded over 80% improvement.

In the case of erythema, male and female improvement was improved by 31.8% and 17.7%, respectively, with respect to general MD7, and male and female improvement was improved by 87.6% and 78.7%, respectively. Effect. In the case of skin scratching, the improvement of male and female improvement was 29.4% and 11.9% for the control MD7, respectively, and the improvement of male and female for MD7 with oyster oak extract was 60.0% and 66.5%, respectively. Improved effect. In the case of skin dryness, the improvement of male and female was improved by 21.9% and 8.0% for MD7, the control, respectively. The improvement of male and female was 60.0% and 70.6% for MD7 with oak oak extract, respectively. Improved effect. In the case of itching, the improvement of male and female for MD7 control was 31.8% and 17.7%, respectively. The improvement of male and female for MD7 with oyster oak extract was 63.6% and 82.4%, respectively. It showed an improvement effect. In the case of sleep disorders, the male and female improvements for the control group MD7 were 19.2% and 29.4%, respectively, and the male and female improvements for MD7 with oyster oak extract were 71.4% and 100%, respectively. Improved effect.

As a result of comprehensive evaluation on MD7 atopy lesions of Oyster oak extract added, it showed numerically higher alleviation effect than MD7, a control, and it could be used as an adjunct to existing treatments for sleep disorder caused by itching. In addition, the possibility of high effects was confirmed due to the possibility of long-term use. In addition, the effect of reducing atopic dermatitis and nodular pruritus was shown to be a good effect and higher than that of a single treatment.

Example 7 Histamine Reaction Test

To test the histamine response to atopic patients, a rectangular grid of 3 × 3 cm was marked on the inside of 40 volunteer arms (10 cm from the wrist) and divided into test and control groups. A solution of 3 g of 95% ethanol extract and 100 ml of 80% ethanol was applied to a square grid three times a day for 1 ml each, and the histamine reaction was examined. At this time, the control was 1 ml of 80% ethanol. After 15 minutes, the histamine solution (Bencard ^® , UK) and the control solution were applied to the lightly scraped area twice with an Allergy lancet (JN Eberle GmBH, Schwabmuenchen, Germany). The average value was evaluated.

Diameter, mm rash Erythema Control  6.95 ± 0.38a *  30.30 ± 1.36a * Test 5.52 ± 0.42b 24.68 ± 1.88b

Table 5 shows the results of a histamine reaction in atopic dermatitis patients. The histamine test induced artificial rashes and erythema on the skin, and the oyster oak extract was applied to the skin to observe the antihistamine effect. Skin rashes decreased by approximately 20.5% to 6.95 mm at the site where oak oak extract was not applied and to 5.52 mm at the site where oak oak extract was applied. The erythema was decreased by 18.5% at the oyster oak extract and statistically significant at 5%.

Hereinafter, various prescription examples containing oyster oak extract according to the present invention as an active ingredient will be described in detail, but are not intended to limit the present invention.

<Prescription Example 1> Prescription Example of Cosmetic Composition

Prescription Example 1-1 Preparation of Nutritional Lotion

3.0 parts by weight of propylene glycol, 0.1 parts by weight of carboxypolymer, a preservative amount and a residual amount of purified water were heated to 80-85 ° C. while mixing and stirring, and then an emulsifier was activated. Polysorbate 60 was 1.0 parts by weight, sorbitan. 0.5 parts by weight of sesquioleate, 10.0 parts by weight of paraffin, 1.0 parts by weight of sorbitan stearate, 0.5 parts by weight of lipophilic monostearic acid, 1.5 parts by weight of stearic acid, 1.0 parts by weight of glyceryl stearate / PEG-400 stearate, triethanol 0.2 parts by weight of amine was heated to 80 to 85 ° C. and emulsified. After emulsification, the mixture was thermally cooled to 50 ° C. while stirring using a stirrer, and then a small amount of flavor was added thereto, followed by cooling to 45 ° C., followed by a small amount of dye. After cooling down to aging.

<Prescription 1-2> Preparation of nutrition cream

0.3 parts by weight of carboxypolymer, 5.0 parts by weight of butylene glycol, 3.0 parts by weight of glycerin and the remaining amount of purified water were heated to 80 to 85 ° C. while stirring, and then added to the production part to act on an emulsifier, 2.0 parts by weight of stearic acid, cetyl alcohol 2.0 parts by weight, glyceryl monostearate 2.0 parts by weight, polyoxyethylene sorbitan monostearate 0.5 parts by weight, sorbitan sesquioleate 0.5 parts by weight, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 part by weight, 1.0 part by weight of wax, 4.0 parts by weight of liquid paraffin, 4.0 parts by weight of squalane, 4.0 parts by weight of caprylic / capric triglycerides were heated to 80 to 85 ° C., and 0.5 parts by weight of triethanolamine was added thereto to emulsify. . After emulsification, the mixture was cooled to 35 ° C. while stirring using a stirrer, and the oyster oak extract according to Example 1 was added thereto, cooled to 25 ° C., and aged.

<Prescription Example 1-3> Preparation of Wash Foam

30.0 parts by weight of TEA-cocoyl glutamate, 10.0 parts by weight of disodium laureth sulfosuccinate glycerin, 10.0 parts by weight of glycerin, 2.0 parts by weight of cocamide DEA, 1.0 parts by weight of PEG-120 methylglucose dioleate, methylgluse- 20 0.5 parts by weight, PEG-150 pentaerythryl tetrastearate, 0.5 parts by weight of tetrasodium EDTA and 0.05 parts by weight of preservative were sequentially added to the preparation portion, heated to 60 to 65 ℃ and stirred for 15 minutes. After stirring, a part of purified water was added and mixed for 30 minutes, and then a part of purified water was slowly added, stirred for 30 minutes, cooled to 35 ° C, and oyster oak extract and spices according to Example 1 were added to 25 After cooling to ℃ it was aged.

<Prescription Example 2> Prescription Example of Pharmaceutical Composition

<Prescription Example 2-1> Preparation of Tablet

Oyster oak extract according to Example 1, corn starch 100 mg, lactose 100 mg, magnesium stearate 2 mg and the remaining amount of purified water were mixed and then tableted according to the manufacturing method of the conventional tablets to prepare a tablet.

<Prescription Example 2-2> Preparation of an ointment

Preparation of a conventional ointment after mixing oyster oak extract 30 mg, PEG-4000 250 mg, PEG-400 650 mg, white petrolatum 10 mg, methyl paraoxybenzoic acid 1.44 mg, propyl paraoxybenzoic acid 0.18 mg and the remaining purified water An ointment was prepared according to the method.

<Prescription Example 2-3> Preparation of Liquid

Oyster oak extract 3,000 mg, dissolbitol solution (70%) 40,000 mg, sucralose 30 mg, acesulfame potassium 60 mg, propylene glycol 1,000 mg, polyoxy40 hydrogenated castor oil 500 mg, potassium sorbate 130 mg After mixing 25 mg of sodium chloride, 58 mg of citric acid, 26 mg of tutifuruti flavor and the remaining amount of purified water, 100 ml of a liquid formulation was prepared according to a conventional method of preparing a liquid formulation.

<Prescription Example 3> Prescription Example of Functional Food Composition

<Prescription Example 3-1> Preparation of Health Food

20 mg of oyster oak extract according to Example 1, vitamin mixture (70 μg of vitamin A acetate, 1.0 mg of vitamin E, 0.13 mg of vitamin B 1, 0.15 mg of vitamin B 2, 0.5 mg of vitamin B 6, 0.2 μg of vitamin B 12, vitamin C 10 mg, biotin 10 µg, nicotinic acid amide 1.7 mg, folic acid 50 µg, calcium pantothenate 0.5 mg, mineral mixture (ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, potassium monophosphate 15 mg , 55 mg of dibasic calcium phosphate, 90 mg of potassium citrate, 100 mg of calcium carbonate, 24.8 mg of magnesium chloride) were mixed, and then granules were prepared and health food was prepared according to a conventional method.

Prescription Example 3-2 Preparation of Health Beverage

20 mg of oyster oak extract according to Example 1, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate, 1 g of taurine and purified water were added to make the total 900 ml, and the above ingredients were mixed according to a conventional healthy beverage manufacturing method. After stirring and heating at 85 ° C. for about 1 hour, the resulting solution was filtered, taken in a sterile 2 L container, sealed sterilized and then refrigerated.

Claims (9)

A composition for improving atopic dermatitis, which comprises an oyster oak extract extracted from a fired oyster oak wood chip as a solvent. The composition for atopic dermatitis improvement of claim 1, wherein the explosive treatment is steamed at a pressure of 20 to 30 kg / cm for 2 to 15 minutes. The composition for improving atopic dermatitis according to claim 2, wherein the explosive treatment is steamed at a pressure of 25 kg / cm for 10 minutes. The composition for improving atopic dermatitis according to claim 1 or 2, wherein the oyster oak extract is a hot water extract or an aqueous ethanol extract. The composition for improving atopic dermatitis according to claim 4, wherein the oyster oak extract is extracted by adding 10 to 50 parts by weight of a solvent based on 1 part by weight of oyster oak. The composition of claim 1 or 2, wherein the composition is hyaluronic acid, cetearyl alcohol, beeswax, cetearyl glucoside, jojoba seed oil, apricot seed oil, burdock extract, lemon extract, hop extract, oleracena extract, sage leaf Extract, Calendula flower extract, Soap grass extract, Cinnabar tree fruit extract, Pasqueflower extract, Esony extract, Disodium adenosine triphosphate, Algin, Papaya fruit extract, Portulaca extract, Polyacrylate-13, Polyisobutene, Polysor Bates, Sodium Polyacrylate, Ethylhexyl Stearate, Trideces-6, Disodium ID, Potassium Hydroxide, Glyceryl Stearate, Fiji-100 Stearate, Squalane, Dimethicone, Niacinamide, Toco It further comprises any one or two or more selected from the group consisting of peryl acetate and dipotassium glycidase Atopic dermatitis improvement composition, characterized in that. The composition for improving atopic dermatitis according to claim 1 or 2, wherein the composition is selected from a cosmetic composition, a pharmaceutical composition or a functional food composition. The group of claim 7 wherein the cosmetic composition comprises a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Atopic dermatitis improving composition, characterized in that it has a formulation selected from. The composition for improving atopic dermatitis according to claim 8, wherein the cream comprises 0.1 to 10.0 parts by weight of oyster oak extract based on 100 parts by weight of a moisturizing agent.

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