KR101330411B1 - Composition for improving atopic dermatitis comprising extract of steamed green tea - Google Patents

Abstract

The present invention relates to a composition for improving atopic dermatitis, which comprises steamed green tea extract as an active ingredient, in particular, the steamed green tea extract exhibits an atopic dermatitis improving effect through histamine test, and is non-toxic even for long time use. Therefore, the composition with this as an active ingredient can be usefully used in the development of cosmetics, drugs and foods for improving atopic dermatitis.

Description

Composition for improving atopic dermatitis comprising extract of steamed green tea}

The present invention relates to a composition for improving atopic dermatitis comprising steamed green tea extract as an active ingredient.

Atopy is a type of hypersensitivity that has recently become increasingly dangerous as the prevalence increases rapidly. From an immunological point of view, atopy is caused by the immune response of immunoglobulin E and allergin. It is defined as a group of allergic diseases with strong genetic tendencies with major symptoms.

In particular, atopic dermatitis, a typical symptom of atopic dermatitis, is estimated to be related to genetic factors and immune system deficiency, although 0.5% -1% of the population and 5-10% of children complain of severe symptoms. The exact cause has not been determined yet, but it is expected to be somewhat alleviated by improving the environment and diet, and there is no fundamental treatment.

However, atopic dermatitis, although showing individual differences, mostly involves severe itching, including dry skin, rashes, rashes, scabs, scaly skin (human scales), and emotional anxiety, stress, tension, and frustration. In addition to feelings of anger and other allergic diseases such as hives, metal allergies, asthma and allergic rhinitis, it is a serious social problem that threatens national health today.

Meanwhile, representative drugs known to be effective in atopic dermatitis to date include steroids, antihistamines, antibiotics, nonsteroidal and other sedatives and neuro stabilizers.

Of these, steroids have excellent effects on anti-inflammatory and immunosuppressive effects, but when used for long periods of time, the elasticity of the skin disappears and becomes thinner, resulting in unpleasant bleeding. In severe cases, hormonal systemic symptoms, adrenal function and immunity decrease. Appears, there are disadvantages that the symptoms explode worse when you stop the drug.

In addition, antihistamines are only temporary measures to alleviate the symptoms of itching by preventing histamine from being released from mast cells, and when used for a long time, side effects such as insomnia and anorexia appear.

In addition, antibiotics are intended to prevent secondary bacterial infections caused by scratching, rather than being a fundamental therapeutic drug for atopic dermatitis.In addition to increased resistance and decreased gastrointestinal function with prolonged use, hepatic damage and gastric colitis, Can cause aplastic anemia due to bone marrow function.

In addition, other nonsteroidal drugs are only moisturizers for the relief of symptoms of atopic dermatitis, and sedatives and neurostabilizers are only supplements for symptomatic relief, rather than fundamental treatment.

Moreover, these existing atopy therapies differ depending on the individual's constitution, and premise of the addition and use of artificial chemical compositions, especially during the composition and manufacturing process, and therefore, unlike the pharmacological components, they may act as serious allergens in atopic patients. This is very large and it is difficult to trust its safety.

Therefore, the present inventors have 5 to 8 times more vitamin C than lemon, and thus, among the green tea extracts that have been reported to be effective in preventing skin aging by inhibiting blemishes and freckles and sedating the skin and removing harmful oxygen. The present invention was completed by confirming that the green tea extract obtained by different steaming pretreatment and extraction solvent before extraction showed an improvement effect on atopic dermatitis in histamine test.

Accordingly, it is an object of the present invention to provide a composition for improving atopic dermatitis, which contains a steamed green tea extract as an active ingredient that can exhibit an improvement effect on atopic dermatitis.

In addition, another object of the present invention is to provide a cosmetic composition, pharmaceutical composition and functional food composition for improving atopic dermatitis, which contains steamed green tea extract as an active ingredient, which may exhibit an improvement effect on atopic dermatitis.

In order to achieve the above object, the present invention provides a composition for improving atopic dermatitis containing an extract selected from the group consisting of hot water extract of steamed green tea, ethanol extract of steamed green tea and a mixture of these extracts as an active ingredient. .

The mixture may further include any one or two or more extracts of hot water extract of steam untreated green tea or ethanol extract of steam untreated green tea, wherein the green tea is any one of the leaves of unused green tea or the branches of green tea or It can contain more than one.

Hot water extract of green tea according to the present invention comprises the steps of steaming leaves or branches of unused green tea; Adding distilled water to the steamed green tea; Hydrothermal extraction; Filtering; Concentrating; And it can be obtained through a step of lyophilization, the hot water extraction step may be hot water extraction for 30 to 120 minutes at 100 to 130 ℃, preferably at 121 ℃ for 30 minutes.

In addition, the ethanol extract of green tea according to the present invention comprises the steps of steaming leaves or branches of unused green tea; Adding and extracting ethanol or an aqueous ethanol solution to steamed green tea; Filtering; Concentrating; And lyophilizing, wherein the aqueous ethanol solution is used in an aqueous 70-99% ethanol solution, and the extraction step is performed for 8 to 7 days at room temperature or boiling temperature, preferably at 25 ° C. for 48 hours. While extracting at 100 rpm.

The steaming treatment is preferably steamed at 121 ° C. for 30 to 60 minutes. If the steam treatment condition is out of the temperature range and the time range, problems of destruction of the active ingredient, energy efficiency, and lowering of extraction yield may be caused.

The composition according to the present invention may include 0.1 to 99.9% by weight of any one or two or more of the hot water extract of steamed green tea or the ethanol extract of steamed green tea, wherein the hot water extract or steam of green tea not steamed It may further include an extract selected from the ethanol extract of untreated green tea, and if the extract is included outside the content range, atopic dermatitis improvement effect may be insignificant or other side effects may be caused.

According to one embodiment of the invention, the composition is a hot water extract of steamed green tea, ethanol extract of steamed green tea, steamed untreated hot water extract and ethanol extract of steamed green tea, respectively 1: 1 It may be included in a weight ratio of 1: 1.

The composition according to the present invention is hyaluronic acid, cetearyl alcohol, beeswax, cetearyl glucoside, jojoba seed oil, apricot seed oil, burdock extract, lemon extract, hops extract, red pepper extract, sage leaf extract, calendula flower Extract, Soap Extract, Cinnabar Fruit Extract, Pasqueflower Extract, Essonia Extract, Disodium Adenosine Triphosphate, Algin, Papaya Fruit Extract, Portulaca Extract, Polyacrylate-13, Polyisobutene, Polysorbate, Sodium Polyacrylic Ethyl, Ethyl Hexyl Stearate, Trideces-6, Disodium Ithithiate, Potassium Hydroxide, Glyceryl Stearate, PIG-100 Stearate, Squalane, Dimethicone, Niacinamide, Tocopheryl Acetate and Dipotassium It may further comprise any one or two or more selected from the group consisting of glycyrize.

In addition, the composition of the present invention may be provided in various forms selected from cosmetic compositions, pharmaceutical compositions or functional food compositions.

When the composition of the present invention is a cosmetic composition, the formulation of the cosmetic composition may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, Powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like can be formulated as, but are not limited to. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

Preferably, the cosmetic composition may be in the form of a cream containing 0.1 to 10.0 parts by weight of an extract selected from the group consisting of hot water extract of steamed green tea, ethanol extract of steamed green tea, and a mixture of these extracts, based on 100 parts by weight of a moisturizing agent. Can be.

When the formulation of the cosmetic composition is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizer or an emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid. Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the composition of the present invention is a pharmaceutical composition, the pharmaceutical composition may include a pharmaceutically acceptable carrier in addition to the green tea extract, such a pharmaceutically acceptable carrier is commonly used in pharmaceutical formulations, Lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methyl hydride Hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like, but are not limited thereto. In addition, the pharmaceutical composition may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. as an additive.

The pharmaceutical composition may be administered according to the severity of symptoms of atopic dermatitis. Typically, topical administration is preferred. The dosage of the active ingredient in the pharmaceutical composition may vary depending on the route of administration, the severity of the disease, the age, sex, and weight of the patient, and may be administered once to several times per day.

The pharmaceutical compositions may be prepared in unit dose form or formulated using pharmaceutically acceptable carriers and / or excipients or may be prepared within a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion, or may be in the form of an exercicide, extract, powder, granule, tablet, warning, lotion, ointment, or the like.

In addition, when the composition of the present invention is a functional food composition, the functional food composition may be prepared from gums, vitamin complexes, dietary supplements, special nutritional supplements, functional drinks and the like.

In addition to the green tea extract as an active ingredient, the functional food composition of the present invention, glucose, monosaccharides such as fructose, disaccharides such as maltose, sucrose, or polysaccharides such as dextrin, cyclodextrin may be added, xylitol Sugar alcohols such as sorbitol, erythritol and the like can also be added, and sweeteners such as taurine, stevia extract and the like can also be added.

When the composition of the present invention is added to food for the purpose of improving atopic dermatitis, it may generally be added at 1 to 20% by weight of the total food weight, and when added to a beverage, 0.1 to 100 mL based on 100 mL , Preferably in an amount of 10 to 100 mL.

On the other hand, hot water extract or ethanol extract of steamed green tea used in the composition of the present invention is a natural product, there is little toxicity and side effects can be used safely even when taking long-term for the purpose of prevention.

According to the present invention, the composition of the present invention containing the steamed green tea extract as an active ingredient shows an atopic dermatitis improvement effect through histamine test, etc., a composition for improving atopic dermatitis using only natural products as an active ingredient without using chemicals Therefore, it can be usefully used in the development of cosmetics, drugs and foods for improving atopic dermatitis.

1 is a flowchart illustrating a process of obtaining each green tea extract according to the present invention;
Figure 2 shows the gallic acid standard calibration curve based on the Folin-Ciocalteau method,
Figure 3 is an analysis of the antioxidant activity of each green tea extract according to the present invention,
4 shows a model for assessing histamine response,
Figure 5 shows the atopic improvement effect of the cream containing each green tea extract according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited by these examples.

Example 1 Preparation of Green Tea Extract

1. Disclosure Material

The tea leaves and branches used in this experiment were harvested from the tea plant at Hadong Green Tea Research Institute, 539, Buchun-ri, Hwagae-myeon, Hadong-gun, Gyeongnam, Korea, in September 2009, and stored at -75 ℃ cold storage.

2. Steam pretreatment

The tea leaves and branches stored in the cold storage were dried at room temperature and steamed at 121 ° C. for 30 to 60 minutes. At this time, tea leaves and branches without steaming were used as a control.

3. Extract Manufacturer

In order to separate the effective extracts of green tea, hot water extracts and ethanol extracts were prepared using hot water extraction and ethanol extraction, respectively. In other words, the control and the previously steamed steam treatment sample was added to 50 g (dry weight basis) of each 1000 mL of distilled water and reacted for 30 minutes at 121 ℃ using 2G3 Glass-filter (PYREX ^® 32940 FNL, JAPAN) After filtration, hot water extracts were obtained.

In addition, 20% of 95% ethanol was added to 20 g of the control and the steaming treatment samples previously steamed, and extracted for 48 hours in a 25 ° C shaking incubator, followed by filtering with 2G3 Glass-filter to obtain an ethanol extract ( 1).

These four extracts were dried to a certain level in a rotary concentrator (BUCHI, SWITZERLAND) at 50 ° C., and then lyophilized using a freeze dryer (Ilshin, Korea), and then each extract powder was obtained. The yield was measured based on Equation 1 below.

[Equation 1]

As a result, the hot water extract of green tea showed yellow color, the ethanol extract of green tea showed dark brown color, and the hot water extract of the sample without steam treatment showed the highest yield of 7.5%, even in the steamed hot water extract. 6.9% yielded similar yields. On the other hand, the steamed ethanol extract showed the lowest yield of 1.7%, and the ethanol extract of the sample without steam treatment showed 2.2%, which was lower than the two hot water extracts. Therefore, as an optimum extraction method for obtaining a high extract amount, it was confirmed that hot water extract is advantageous.

extract Yield (%) Hot water extract - 7.5 Ethanol extract - 2.2 Hot water extract Steam pretreatment 6.9 Ethanol extract Steam pretreatment 1.7

Example 2 Content Analysis of Polyphenols

The polyphenol content of the green tea extract prepared in Example 1 was analyzed according to the Folin-Ciocalteu method (Analytica Chimica Acta 2007; 592 (2): 193-201). 30 μl of gallic acid (SIGMA, USA) (1 mg / mL) is mixed with 3 mL of distilled water, then 100 μl of Folin-Ciocalteu (SIGMA, USA) is added for 5-8 minutes and then 300 μl of sodium carbonate The mixture was reacted for 30 minutes and the absorbance was measured at 765 nm using a UV-spectrometry (U-3000 ^® , Hitachi, Japan) to prepare a calibration curve (FIG. 2). The extract powders prepared in Example 1 were also measured for absorbance in the same manner and analyzed for the content of polyphenols in each green tea extract compared to the calibration curve (Table 2).

As a result, as shown in Table 2, the highest polyphenol content was found to be 354.1 mg / g in the hot water extract of the sample without steaming, and the polyphenol content of 262.1 mg / g in the ethanol extract of the steaming sample. Although the ethanol extract of the sample that was not steamed was 251.4 mg / g, it showed the lowest polyphenol content.

To obtain the maximum polyphenol content, it is advantageous to extract hot water from un Steamed natural leaves and branches. To obtain a large amount of polyphenols by ethanol extraction, it is better to steam and then extract the tea leaves and branches beforehand. Was judged.

extract Polyphenol Content (mg / g) Hot water extract - 354.1 ± 0.2 Ethanol extract - 251.4 ± 0.1 Hot water extract Steam pretreatment 281.2 ± 0.2 Ethanol extract Steam pretreatment 262.1 ± 0.2

Example 3 Antioxidant Activity Assay (DPPH Radical Scavenging Activity Review)

Dilute (100 ppm) each green tea extract prepared in Example 1 to a 96 well plate (FALCON ^® , USA) and add 50 μl of 0.2 mM DPPH (2,2-diphenyl-1-picryl-hydrazyl, Sigma, USA). ) 200 μl of the solution was reacted at room temperature for 30 minutes, and the absorbance was measured at 517 nm by ELISA (Anthos ^® , Austria). At this time, the control was measured using the same method using BHT (Butylated Hydroxy Toluene, Sigma, USA) or 100% ethanol as an antioxidant.

As a result, as shown in FIG. 3, 48% of the antioxidant activity was observed in the hot water extract of the sample that was not steamed, and this value was the highest in the four extracts. The extract also showed 45% antioxidant activity.

When the four extracts were compared with the control group BHT and the antioxidant activity, it was found that the antioxidant activity was slightly lower. The ethanol extracts of the steamed samples and the ethanol extracts of the steamed samples were 37% and 34%, respectively. It showed antioxidant activity.

In other words, it was confirmed that the hot water extract showed higher antioxidant activity than the ethanol extract, and the hot water extract obtained from the leaves and branches of the natural state and the hot water extract of the steamed sample were expected to have a similar bioactive effect as the synthetic antioxidant. Could.

Example 4 Histamine Response Evaluation

In order to evaluate the histamine response to atopic patients, a circle with a diameter of 3 cm was marked on the inside of the arm of the clinical volunteer (10 cm away from the wrist), and then divided into a control group and a control group (FIG. 4). 5 g of each green tea extract prepared in Example 1 was dissolved in 100 mL of 80% ethanol and applied to the prototype three times a day 1 mL. At this time, the control group used 80% ethanol without the extract, and the extract mixture was the hot water extract of steamed green tea, the ethanol extract of steamed green tea, the hot water extract of steamed green tea and the ethanol of uncooked green tea. It means that the extract is included in a weight ratio of 1: 1: 1: 1, respectively.

The longest and shortest diameters of the rash and erythema were measured after 15 minutes by dropping the histamine solution (Ben card ^® , UK) and the control solution on the site where the skin reaction test was scratched twice. (FIG. 4).

As a result, skin rashes of 6.55 ± 1.42 mm were formed at the site where 80% ethanol was applied as a control in skin rashes as shown in Table 3 below, but 6.07 ± 1.04 mm in the hot water extract of steamed samples The skin rash of 5.68 ± 1.20 mm was formed in the ethanol extract of, and it can be seen from these results that the hydrothermal and ethanol extracts of the steamed samples showed anti-inflammatory effects.

In skin erythema, 25.83 ± 6.34 mm of skin erythema was formed at the site of 80% ethanol applied as a control, and 19.72 ± 6.18 mm at the site of ethanol extract of steamed sample, and skin erythema was about 6.11. The percentage was reduced and significant at the 5% level. From these results, it can be seen that the ethanol extract of the steamed sample is effective in reducing skin erythema.

extract Diameter (mm) rash Erythema Hot water extract 6.43 ± 1.49 24.75 ± 5.10 Ethanol extract 6.99 ± 1.21 25.06 ± 5.91 Steam pretreatment hot water extract 6.07 ± 1.04 25.88 ± 6.84 Pretreated Ethanol Extract 5.68 ± 1.20 19.72 ± 6.18 Extract mixture 6.24 ± 1.10 24.85 ± 7.35 80% ethanol 6.55 ± 1.42 25.83 ± 6.34 Negative control group 4.30 ± 1.10 11.79 ± 6.43

In addition, as a result of examining the antihistamine effect according to the sex of men and women, as shown in Table 4, it was not judged as effective in comparison with the control 80% ethanol (6.18 mm) in the skin rash in men, 80 in the skin erythema Compared to% ethanol (25.12 mm), the ethanol extract of steamed samples was 17.88 ± 5.53 mm, which reduced skin erythema by 28.8%.

In the skin rash of women, the rash remission effect was shown to be 5.85 mm and 5.40 mm in the hot water extract and ethanol extract of steamed sample when comparing 80% ethanol (6.78 mm) with each extract. These figures were significant at the 5% level. In erythema of women, the ethanol extract of steamed sample showed 20.85 mm erythema reduction effect compared to the control 80% ethanol (26.26 mm), which was significant at 5%.

Therefore, it was found that the ethanol extracts of the steamed samples commonly exhibited the antihistamine effect in the male and female histamine reactions.

extract Diameter (mm) male female rash Erythema rash Erythema Hot water extract 6.67 ± 0.92 26.08 ± 5.16 6.29 ± 1.77 23.93 ± 5.87 Ethanol extract 7.43 ± 1.61 25.47 ± 5.89 6.72 ± 0.83 24.80 ± 6.14 Steam pretreatment hot water extract 6.41 ± 0.94 26.46 ± 7.11 5.85 ± 1.07 25.52 ± 6.93 Pretreated Ethanol Extract 6.14 ± 0.74 17.88 ± 5.53 5.40 ± 1.36 20.85 ± 6.50 Extract mixture 6.80 ± 1.26 24.76 ± 8.74 5.89 ± 0.86 24.91 ± 6.74 80% ethanol 6.18 ± 0.93 25.12 ± 6.41 6.78 ± 1.64 26.26 ± 6.51 Negative control group 4.84 ± 1.09 10.13 ± 5.86   3.96 ± 0.99 12.82 ± 6.77

In addition, as a result of examining the antihistamine effect according to the smoking, as shown in Table 5.

That is, in the experiments with smokers, the five extracts did not show a rash inhibiting effect compared to the control 80% ethanol (5.59 mm).

In the skin erythema experiment, the ethanol extract (20.36 mm) of the steamed sample had an inhibitory effect on erythema compared to the control group, 80% ethanol (25.89 mm).

In non-smoker experiments, hot water extract and ethanol extract of steamed samples were 6.10 mm and 5.64 mm, respectively, compared with the control group, 80% ethanol (6.85 mm). Was significant in.

In skin erythema experiments in non-smokers, the steamed ethanol extract was 19.52 mm, which was effective compared to the control 80% ethanol (25.81 mm).

Therefore, it was found that the steamed ethanol extract showed an inhibitory effect on skin rash and erythema except for the rash of the smoker experiment compared to the control.

extract Diameter (mm) smoker Non-smoker rash Erythema rash Erythema Hot water extract 6.36 ± 0.82 27.92 ± 5.02 6.46 ± 1.66 23.76 ± 4.85 Ethanol extract 6.69 ± 1.39 26.71 ± 4.26 7.08 ± 1.17 24.54 ± 6.36 Steam pretreatment hot water extract 5.95 ± 0.41 28.91 ± 5.21 6.10 ± 1.18 24.93 ± 7.14 Pretreated Ethanol Extract 5.83 ± 0.73 20.36 ± 5.40 5.64 ± 1.33 19.52 ± 6.56 Extract mixture 6.17 ± 0.99 26.61 ± 7.52 6.26 ± 1.16 24.30 ± 7.45 80% ethanol 5.59 ± 0.28 25.89 ± 3.89 6.85 ± 1.50 25.81 ± 7.04 Negative control group 4.47 ± 0.83 9.17 ± 6.47 4.24 ± 1.19 12.61 ± 6.40

< Example  5> Preparation and Clinical Experiment of Atopy Cream

In order to prepare atopy creams, each of the green tea extracts prepared in Example 1 was concentrated at a ratio of 1g / 10 mL, and each of the commercially available moisturizers (purified water, glycerin, isohexadecane, triethylhexa) was used with a total of five extracts. Elderly, sodium hyaluronate, stearic acid, cetyl alcohol, ethyl alcohol, sorbitan stearate, sucrose cocoate, glyceryl acrylate, acrylic acid copolymer, propylene glycol, dimethicone, polysorb 100 g of bait 60, tocopheryl acetate, carbomer, sorbitan sesquioleate, triethanolamine, allantoin, methylparaben, imidazolidinyl urea, disodium ethane, dapotassium glycyriate, propylparaben) An atopy formulation containing 1 g of each extract was prepared.

The five atopy creams prepared in this way were commissioned by W dermatologist located in C, Gyeongnam, and examined the effect of improving atopic dermatitis in a total of 100 atopic patients between the ages of 10 and 50. At this time, 20 persons were classified into one experimental group for each treatment group of atopy cream, and the application period of the atopy cream was applied in 6 to 8 weeks. The evaluation of atopic dermatitis improvement effect was based on a survey of subjects and the gross findings of two dermatologists, and the results were (i) very good, (ii) good, (iii) moderate, and (iv) insufficient. Each was analyzed by dividing.

As a result, the green tea ethanol extract steamed as shown in Figure 5 and Table 6 showed the most excellent atopic dermatitis improvement effect.

extract Atopic dermatitis improvement Very good Good usually Inadequate Hot water extract 6 10 4 - Ethanol extract One 5 10 4 Steam pretreatment hot water extract 9 7 4 - Pretreated Ethanol Extract 10 6 4 - Extract mixture 8 8 4 -

Hereinafter, various prescription examples using steamed green tea ethanol extract according to the present invention will be described, which is not intended to limit the present invention but merely to explain in detail.

<Prescription Example 1> Prescription Example of Cosmetic Composition

Prescription Example 1-1 Preparation of Nutritional Lotion

3.0 parts by weight of propylene glycol, 0.1 part by weight of carboxy polymer, purified water of a small amount of preservative and remaining amount were heated to 80 to 85 ° C while stirring and mixing, and then charged into a manufacturing part. Then, an emulsifying agent was allowed to act. 1.0 part by weight of Polysorbate 60, 0.5 parts by weight of sesquioleate, 10.0 parts by weight of liquid paraffin, 1.0 part by weight of sorbitan stearate, 0.5 parts by weight of glycerin monostearate as a parent type, 1.5 parts by weight of stearic acid, 1.0 part by weight of glyceryl stearate / PEG-400 stearate, And 0.2 parts by weight of amine were heated to 80 to 85 DEG C and then emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then a small amount of fragrance was added. The mixture was cooled to 45 ° C., followed by a small amount of pigment. After aging.

<Prescription 1-2> Preparation of nutrition cream

0.3 parts by weight of carboxy polymer, 5.0 parts by weight of butylene glycol, 3.0 parts by weight of glycerin and the remaining amount of purified water were heated to 80 to 85 캜 while stirring and mixing, 2.0 parts by weight, glyceryl monostearate 2.0 parts by weight, polyoxyethylene sorbitan monostearate 0.5 part by weight, sorbitan sesquioleate 0.5 part by weight, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 part by weight of wax, 4.0 parts by weight of liquid paraffin, 4.0 parts by weight of squalane, and 4.0 parts by weight of caprylic / capric triglyceride were heated to 80 to 85 ° C. and 0.5 part by weight of triethanolamine was added thereto to emulsify . After emulsification, the mixture was cooled to 35 ° C. while stirring using a stirrer, and steamed green tea ethanol extract was added thereto, cooled to 25 ° C., and aged.

<Prescription Example 1-3> Preparation of Wash Foam

30.0 parts by weight of TEA-cocoyl glutamate, 10.0 parts by weight of disodium laureth sulfosuccinate glycerin, 10.0 parts by weight of glycerin, 2.0 parts by weight of cocamide DEA, 1.0 parts by weight of PEG-120 methylglucose dioleate, methylgluse- 20 0.5 parts by weight, PEG-150 pentaerythryl tetrastearate, 0.5 parts by weight of tetrasodium EDTA and 0.05 parts by weight of preservative were sequentially added to the preparation portion, heated to 60 to 65 ℃ and stirred for 15 minutes. After stirring, part of purified water was added and mixed for 30 minutes. Then, a part of purified water was slowly added, stirred for 30 minutes, cooled to 35 ° C, steamed green tea ethanol extract and flavored were added to 25 ° C. After cooling it was aged.

<Prescription Example 2> Prescription Example of Pharmaceutical Composition

<Prescription Example 2-1> Preparation of Tablet

50 mg of steamed green tea ethanol extract, 100 mg of corn starch, 100 mg of lactose, 2 mg of magnesium stearate and the remaining amount of purified water were mixed and tableted according to a conventional method for preparing tablets.

<Prescription Example 2-2> Preparation of an ointment

Steamed green tea ethanol extract 30mg, PEG-4000 250mg, PEG-400 650mg, white petrolatum 10mg, paraoxybenzoic acid methyl 1.44mg, paraoxybenzoic acid propyl 0.18mg and the remaining purified water after mixing the conventional ointment preparation method Thus an ointment was prepared.

<Prescription Example 2-3> Preparation of Liquid

Steamed green tea ethanol extract 3,000mg, dissolbitol solution (70%) 40,000mg, sucralose 30mg, acesulfame potassium 60mg, propylene glycol 1,000mg, polyoxy40hydrogenated castor oil 500mg, potassium sorbate 130mg, sodium chloride After mixing 25 mg, citric acid 58 mg, tutifuruti flavor 26 mg and the remaining amount of purified water, 100 ml of a liquid formulation was prepared according to a conventional method for preparing a liquid formulation.

<Prescription Example 3> Prescription Example of Functional Food Composition

<Prescription Example 3-1> Preparation of Health Food

20 mg of steamed green tea ethanol extract, vitamin mixture (70 μg of vitamin A acetate, 1.0 mg of vitamin E, 0.13 mg of vitamin B 1, 0.15 mg of vitamin B 2, 0.5 mg of vitamin B 6, 0.2 μg of vitamin B 12, vitamin C 10 Mg, biotin 10 μg, nicotinic acid amide 1.7 mg, folic acid 50 μg, calcium pantothenate 0.5 mg, mineral mixture (ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, potassium monophosphate 15 mg, agent 55 mg of calcium diphosphate, 90 mg of potassium citrate, 100 mg of calcium carbonate, 24.8 mg of magnesium chloride) were mixed, and then granules were prepared and health food was prepared according to a conventional method.

&Lt; Prescription Example 3-2 > Preparation of health drink

20 mg of steamed green tea ethanol extract, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate, 1 g of taurine, and purified water were added to make it total 900 ml, and the above ingredients were mixed according to a conventional healthy beverage manufacturing method. After stirring and heating at 85 ° C. for about 1 hour, the resulting solution was collected by filtration into a sterilized 2 L container, sealed, sterilized, and refrigerated.

Claims (9)

A pharmaceutical composition for improving atopic dermatitis, containing ethanol extract of steamed green tea treated at 121 ° C. for 30 to 60 minutes as an active ingredient. The method according to claim 1, wherein the pharmaceutical composition is any one selected from the group consisting of hot water extract of steamed green tea steamed for 30 to 60 minutes at 121 ℃, hot water extract of steamed green tea and ethanol extract of steamed green tea Atopic dermatitis improving pharmaceutical composition, characterized in that it further comprises an extract. The method of claim 2, wherein the pharmaceutical composition is hot water extract of steamed green tea, ethanol extract of steamed green tea, steamed untreated hot water extract and ethanol extract of steamed green tea, respectively, 1: 1: 1: Atopic dermatitis improvement pharmaceutical composition, characterized in that it comprises a weight ratio of 1. The pharmaceutical composition for improving atopic dermatitis according to claim 1 or 2, wherein the green tea comprises any one or two or more of leaves or green tea branches. delete The pharmaceutical composition of claim 1, wherein the pharmaceutical composition is hyaluronic acid, cetearyl alcohol, beeswax, cetearyl glucoside, jojoba seed oil, apricot seed oil, burdock extract, lemon extract, hop extract, red pepper extract, sage Leaf Extract, Calendula Flower Extract, Soap Grass Extract, Cinnabar Fruit Extract, Pasqueflower Extract, Essony Extract, Disodium Adenosine Triphosphate, Algin, Papaya Fruit Extract, Portulaca Extract, Polyacrylate-13, Polyisobutene, Poly Sorbate, Sodium Polyacrylate, Ethylhexyl Stearate, Trideces-6, Disodium IDT, Potassium Hydroxide, Glyceryl Stearate, Fiji-100 Stearate, Squalane, Dimethicone, Niacinamide, It further comprises any one or two or more selected from the group consisting of tocopheryl acetate and dipotassium glycidase Atopic dermatitis improvement pharmaceutical composition, characterized in that. delete delete delete

Images