KR102015300B1 - Cosmetic composition comprising actinidia polygama extract for absorbing ultraviolet or protecing skin from ultrviolet - Google Patents

Abstract

Disclosed herein is a cosmetic composition for absorbing ultraviolet rays comprising the extract of Actinidia polygama as an active ingredient. In addition, the present disclosure discloses a cosmetic composition comprising Actinidia polygama extract as an active ingredient and inhibiting skin damage caused by ultraviolet rays and regenerating skin cells damaged by ultraviolet rays to have a skin protection effect against ultraviolet rays.

Description

Cosmetic composition for absorbing ultraviolet rays or skin protection against ultraviolet rays comprising the extract of Lichen ostrich {COSMETIC COMPOSITION COMPRISING ACTINIDIA POLYGAMA EXTRACT FOR ABSORBING ULTRAVIOLET OR PROTECING SKIN FROM ULTRVIOLET}

Disclosed herein is a cosmetic composition for protecting the skin from ultraviolet rays or ultraviolet rays comprising the extract of the wormwood as an active ingredient.

In general, ultraviolet rays contained in sunlight may cause erythema formation or blemishes or blemishes when excessively irradiated directly to the skin, or may cause skin trouble by generating lipid peroxide in response to sebum secreted in the epidermis. If severe, it can also cause skin cancer. UV rays are classified into UV-A (320-400 nm), UV-B (280-320 nm), and UV-C (200-280 nm) depending on the wavelength. Are known as UV-A and UV-B.

The sunscreens used for the purpose of preventing skin damage by the above-mentioned ultraviolet rays are classified into chemical sunscreens and physical sunscreens. Chemical blocking agents that act as chemical absorption of ultraviolet rays are well known organic blocking agents such as cinnamic acid, salicylic acid, and benzophenone, and physical blocking agents that act as physical scattering and shielding of ultraviolet rays are inorganic such as titanium dioxide and zinc oxide. Blockers are well known.

Chemical blockers have the advantage of excellent UV protection, but has a disadvantage of causing skin irritation because the wavelength range is narrow and applied to cosmetics because they are absorbed into the skin in a sticky and greasy feeling and molecular form. In the case of physical blockers, skin irritation is relatively low compared to chemical blockers but has the disadvantage of causing heavy feeling of use, whitening and phototoxicity. On the other hand, the natural sunscreen ingredients with excellent skin safety has a low direct UV protection effect has a disadvantage that must be used by mixing the natural synthetic extract with conventional synthetic organic sunscreen components, not a single natural extract.

Korean Unexamined Patent Publication No. 10-2004-0080640

In one aspect, an object of the present specification is to provide a cosmetic composition for absorbing ultraviolet rays comprising the extract of Actinidia polygama as an active ingredient to improve the disadvantages of conventional sunscreens.

In another aspect, the present specification is to provide a cosmetic composition for protecting the skin against ultraviolet rays comprising the extract of Actinidia polygama as an active ingredient.

In one aspect, the technology disclosed herein provides a UV-absorbing cosmetic composition comprising an extract of Actinidia polygama as an active ingredient.

In another aspect, the technology disclosed herein provides a cosmetic composition for protecting the skin against ultraviolet rays comprising Actinidia polygama extract as an active ingredient.

In an exemplary embodiment, the extract may be a leaf, stem or a mixed extract thereof.

In an exemplary embodiment, the extract may be extracted with one or more solvents selected from the group consisting of water and a container solvent.

In an exemplary embodiment, the organic solvent may be at least one selected from the group consisting of methanol, ethanol, propanol, isopropanol and butanol.

In an exemplary embodiment, the extract may be included at a concentration of 10 to 10,000 ppm with respect to the composition.

In an exemplary embodiment, the extract may have a skin protective effect against ultraviolet rays by absorbing ultraviolet B and inhibiting skin cell damage induced by ultraviolet B irradiation.

In one aspect, the technology disclosed herein has the effect of providing a cosmetic composition for absorbing ultraviolet rays comprising the extract of Actinidia polygama as an active ingredient.

In another aspect, the technology disclosed herein comprises an Actinidia polygama extract as an active ingredient and inhibits the skin damage caused by ultraviolet rays and regenerates skin cells damaged by ultraviolet rays to have a skin protection effect against ultraviolet rays It has the effect of providing a composition.

In another aspect, the extract of Caesar's Efficacy has a significant effect without irritation to the skin, and also has excellent stability of the product after formulation, so that it is very suitable as a cosmetic composition derived from natural products.

Figure 1 is a graph showing the results of experiments in the ultraviolet absorbing ability of the extract Caesar in one test example of the present specification.
Figure 2 is a graph showing the results of testing the cytotoxicity of the extract of Caesar in one test example of the present specification.
Figure 3 is a graph showing the results of experiments on the inhibition of skin cell damage of the extract of Caesar extract in one test example of the present specification.
Figure 4 is a graph showing the results of experiments to promote the skin cell regeneration promoting effect damaged by the ultraviolet rays of the extract of the present invention in one test example of the present specification.
Figure 5 is a graph showing the results of experiments in the antioxidant component analysis of the extract of Caesar in one test example of the present specification.

Hereinafter, the present invention will be described in detail.

In one aspect, the technology disclosed herein provides a UV-absorbing cosmetic composition comprising an extract of Actinidia polygama as an active ingredient.

In another aspect, the technology disclosed herein provides a sunscreen cosmetic composition comprising Actinidia polygama extract as an active ingredient.

In another aspect, the technology disclosed herein provides a cosmetic composition for protecting the skin against ultraviolet rays, including Actinidia polygama extract as an active ingredient.

As used herein, the term "active ingredient" alone refers to a component that may exhibit the desired activity alone or together with a carrier having no activity.

Actinidia polygama is a deciduous broad-leaved shrub belonging to Actinidiaceae Actinidia , which is native to Korea, Siberia, and Japan and grows in mountains of 100m to 1,500m. The growing environment grows in good drainage. The height is 5m, the small branches have light brown hairs when they are young, the leaves are ovate, 8-14cm long, 3.5-8cm wide, and the surface is green at first but white or green and white at the time of flowering. It is together and when seeds are formed, it turns from red to green again. Flowers are white, 1.5cm in diameter, hang on the petiole of the upper part of branch, and bloom one to three in one flower stem and have scent. Fruits run in September-October, oval, about 3㎝ in length, and pulp is about aryl with no sweetness. Strange fruit with worms is used for medicinal purposes. The leaves of the forsythia are plants that show insects deceiving insects. The white leaves look like white paint before they bloom. The white leaves gather the pollinators, and the insects gather into the parasitic worm house. Bugs add medicinal effects.

In the present specification, A. polygama can be used without limitation over the entire range of plants such as roots, stems, leaves, berries, flowers and bark for the production of extracts, and the effect of UV absorption or skin protection against UV rays Stems or leaves may be preferred for promotion. In addition, the canine can be used without limitation, such as cultivated, harvested or commercially available.

In an exemplary embodiment, the extract may be an extract obtained through the extraction process itself or powder thereof, the extract. The extraction process can be carried out according to conventional methods used in the art.

In an exemplary embodiment, the extract is meant to include not only crude extracts but also all forms by processing of crude extracts, such as drying, concentration, fractionation, fermentation, and the like. In the case of fractionated extracts, the crude extracts are suspended in a specific solvent and then mixed with a solvent having a different polarity and left to stand. The crude extracts include all fractions obtained by dividing the crude extracts with a sequential solvent. Also obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). Fractions are also included in the extract of the present specification.

In an exemplary embodiment, the extract may be a hydrothermal extract, a polar solvent soluble extract or a non-polar solvent soluble extract.

In an exemplary embodiment, the extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (eg, methanol, ethanol, propanol, butanol, etc.), ethylene, acetone, hexane, ether, chloroform, ethyl acetate, Butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), methylene chloride, dimethyl sulfoxide (DMSO), glycerin, butylene glycol, propylene glycol, dipropylene glycol, methylene chloride, diethyl ether and mixtures thereof It means that it includes both the crude extract extracted by using one or more solvents selected from the group consisting of and fractions obtained by fractionating the crude extract. At this time, the fractions can be fractionated using the solvents listed above.

The method of preparing the extract may include hot water extraction method, dipping extraction method, steam distillation method, cold needle extraction method, reflux cooling method, ultrasonic extraction method, supercritical extraction method, subcritical extraction method, solvent extraction method, elution method, Any method such as compression, high temperature extraction, high pressure extraction, extraction with adsorption resins including XAD and HP-20, fermentation with microorganisms or natural fermentation metabolism can be applied. The number of extraction may be 1 to 5 times, it is preferable to extract three times, but is not limited thereto. In addition, after the extraction may be carried out a method such as distillation under reduced pressure, concentrated under reduced pressure, freeze drying or spray drying.

In an exemplary embodiment, the extract is ground 2 to 3 at 4 to 30 ℃ by pulverizing, and adding at least one extraction solvent selected from 1 to 50 times the volume of the dry weight of water, alcohol of 1 to 5 carbon atoms After stirring for 7 days to extract the active ingredient, the extract may be prepared by concentrating with a vacuum concentrator.

In an exemplary embodiment, the extract is 3 to 7 at 90 to 100 ℃ by pulverizing the leeks and adding 5 to 15 times, or 5 to 10 times, or 10 to 15 times the volume of water of the dry weight After extracting twice for 4 hours or 4 to 6 hours, the resultant is filtered, concentrated under reduced pressure with a rotary concentrator, and lyophilized to prepare a powder.

In an exemplary embodiment, the extract may be included in 0.00001 to 20% by weight based on the total weight of the composition. In another aspect, the extract is at least 0.001 wt%, at least 0.01 wt%, at least 0.1 wt% or at least 1 wt% and at most 15 wt%, at most 10 wt%, at most 5 wt% or 3 wt% based on the total weight of the composition It may be included in% or less. For example, the extract may be included in an amount of 0.01 to 10% by weight based on the total weight of the composition. By including the extract in the above range in the composition, it is possible to implement an excellent ultraviolet absorption and skin protection effect against ultraviolet rays, have a formulation and product stability, and can exert an excellent effect with an optimal content in terms of economics.

In another aspect, the technology disclosed herein provides an antioxidant composition comprising a compound of any one of the following Formula 1 to 3 as an active ingredient.

[Formula 1]

[Formula 2]

[Formula 3]

In an exemplary embodiment, the compound may have ABTS radical scavenging activity.

In an exemplary embodiment, the antioxidant composition may be a cosmetic composition or a food composition.

In addition to the active ingredient, the cosmetic composition of the present invention may further include functional additives and components included in a general cosmetic composition. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. In addition to the other components included, oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation And accelerators, cooling agents, limiting agents, purified water, and the like.

The cosmetic composition is not particularly limited in formulation, and may be appropriately selected as desired. For example, supple cosmetics, astringent cosmetics, nourishing cosmetics, astringent lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, eye cream, nourishing cream, moisturizing cream, hand cream, oil, foundation, essence, nutrition essence, gel Powder, spray, pack, soap, cleansing water, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser may be prepared in any one or more of the formulation selected from, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Example  One. A dog  Preparation of Extract

The leaves and stems of the Actinidia polygama (Siebold & Zucc.) Maxim. Were washed, dried and crushed finely using a grinder. Thereafter, 0.2 kg of pulverized powder was taken, 2,000 ml of distilled water was added thereto, extracted twice at 90 to 100 ° C. for 4 hours, filtered, concentrated under reduced pressure with a rotary concentrator, and lyophilized to prepare an extract.

Test Example 1. UV Absorption Capacity Test

The following experiment was conducted to confirm the ultraviolet absorption effect of the extract of Caesar.

Specifically, the optical density of the extract from Example 1 was measured in the spectral range of 280 to 700 nm using a UV / Vis spectrophotometer.

As a result, as can be seen in Figure 1, during the treatment of the litterworm extract showed a peak in the ultraviolet B (280 to 320 nm) region was confirmed to have a high absorbance. From the results, it can be seen that the extract of the sea breeze has the ability to absorb UV B and protects skin cells against UV B exposure.

Test Example 2 Cytotoxicity Test

The following experiment was conducted to determine whether the extract of Caesar larvae directly affects skin cell death.

Specifically, using DMEM (in 10% FBS, 1% Penicillin / Streptomysin) as a medium, HaCaT cells are seeded in 96 well plate so as to be 1.0 × 10 ⁴ per well, and after 24 hours the above Example Cabbage extract obtained in 1 was treated to the medium by concentration. After 72 hours, MTT reagent was treated with 100 μl of each well and incubated for 4 hours at 37 ° C. in a CO ₂ incubator, and then absorbance was measured at 570 nm.

As a result, as can be seen in Figure 2, as a result of treating the extract of Example 1 according to the present invention, it was confirmed that there is no direct effect on skin cell death even at 100 ppm concentration.

Test Example 3 Inhibition of Skin Cell Damage Induced by Ultraviolet Rays

In order to confirm the effect of inhibiting skin cell damage induced by ultraviolet irradiation of the extract of Caesar extract, the following experiment was conducted.

Specifically, using DMEM (in 10% FBS, 1% Penicillin / Streptomysin) as a medium, HaCaT cells are seeded in 96 well plate so that 1.0 × 10 ⁴ per well, and then the medium is removed after 24 hours The PBS buffer was treated with 50 μl of each well, and the extracts of Caesar extract obtained in Example 1 were treated for each concentration. In addition, after irradiating UV light B with 30 mJ / cm ² intensity, the PBS buffer was removed, the medium was treated by 100 μl of each well, and the extract of Cauliflower extract obtained in Example 1 was treated by concentration. After 24 hours, 50 μl of the LDH reagent was treated in 50 μl of the medium, and the optical density was measured at 490 nm after being allowed to stand at room temperature for 30 minutes.

As a result, as can be seen in Figure 3, as a result of treating the extract of Example 1 according to the present invention, it was confirmed that there is excellent skin cell damage inhibition effect against ultraviolet rays. From this, it was found that the cytotoxicity induced by ultraviolet irradiation is reduced by about 120% by including the extract of the seaweed as an active ingredient.

Test Example  4. Skin cell proliferation promotion test damaged by ultraviolet rays

In order to confirm the effect of promoting skin cell proliferation damaged by UV irradiation of the extract of Caesar extract, the following experiment was carried out.

Specifically, using DMEM (in 10% FBS, 1% Penicillin / Streptomysin) as a medium, HaCaT cells are seeded in 96 well plate so that 1.0 × 10 ⁴ per well, and then the medium is removed after 24 hours The PBS buffer was treated with 50 μl of each well, and the extracts of Caesar extract obtained in Example 1 were treated for each concentration. In addition, after irradiating UV light B with 30 mJ / cm ² intensity, the PBS buffer was removed, the medium was treated by 100 μl of each well, and the extract of Cauliflower extract obtained in Example 1 was treated by concentration. After 24 hours, MTT reagent was treated with 100 μl of each well and incubated for 4 hours at 37 ° C. in a CO ₂ incubator, followed by measurement of optical density at 570 nm.

As a result, as can be seen in Figure 4, as a result of treating the extract of Example 1 according to the present invention, it was confirmed that there is an excellent cell proliferation promoting effect against damage by ultraviolet rays. From this, it was found that the inclusion of the extract of the seaweed as an active ingredient, increased the proliferation of cells inhibited by ultraviolet irradiation by about 20%.

Test Example  5. Raw material stability test

The following experiment was conducted to confirm the stability of the raw material of the extract.

Specifically, the extract of Cauliflower extract obtained in Example 1 was prepared as a solution of 10,000 ppm concentration and stored for 3 months under conditions of 25 ℃, 42 ℃ and cycle (0 ℃ → 25 ℃ → 40 ℃, 8 hours each) After appearance, pH change and microbial contamination were measured.

As a result, the extract of Example 1 was maintained for 3 months in all storage conditions without aggregation, precipitation, discoloration, odor, microbial contamination and pH change. Therefore, it was found that the extract of Caesar's extract according to the present invention has high stability.

Test Example  6. Analysis of Antioxidant Components Using Online Antioxidant Device

The experiment was conducted as follows to identify the compounds having the antioxidant efficacy of the extracts.

The litter blade was dried at room temperature and then ground using a grinder. 100 g of the ground powder was placed in 1,000 ml of 70% EtOH and extracted under reflux for 5 hours. Finally, the extract was extracted three times in total, filtered and concentrated under reduced pressure to prepare 24.31 g of extracts. 20 mg of the prepared extract was dissolved in 1 ml of 70% EtOH and used as a sample of an online antioxidant component analysis system.

ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) reaction reagent was prepared in 2 mM ABTS solution containing 3.5 mM potassium persulfate and stored for 12 hours at room temperature under dark conditions. 734 nm was used as an appropriate wavelength to know the scavenging ability of free radicals, the column was a YMC ODS-A column (4.6 * 150 mm), the detector was UV (254, 280, 330 nm), and the mobile phase was Acetonitrile (A for HPLC grade). ) And water (B) 0-5 minutes, 5% A; 5-15 minutes, 15-5% A; 15-30 minutes, 25-50% A; 30-35 minutes, 50-100% A; 35 The linear gradient was performed at 100% A for ˜40 minutes, and the flow rates were 0.7 ml / min (Acetonitrile, water) and 0.35 ml / min (ABTS solution), and the sample injection amount was 10 μl. Activity was measured at 734 nm as negative peak and shown in FIG. 5.

As can be seen in Figure 5, it was confirmed that the compound with ABTS radical scavenging activity is present in the extract of Caesar. Compounds were identified through compound separation. The separated compounds were identified by chemical analysis using molecular ion analyzer (MS) and nuclear magnetic resonance spectroscopy (NMR).

As a result, the compound of formula 1 was kaempferol-3-O- [a-rhamnopyranosyl- (1-3) -aL-rhamnopyranosyl- (1-6) -aD-galactopyranoside], molecular weight 740, molecular formula C ₃₃ H ₄₀ O ₁₉ The compound of formula 2 is Kaempferol 3-O- [aL-rhamnopyranosyl- (1-3)-(4-O-acetyl) -OaL-rhamnopyranosyl- (1-6) -ObD-galactopyranoside], molecular weight 782, C ₃₅ H ₄₂ O ₂₀ , and the compound of Formula 3 is Kaempferol 3-O- [aL-rhamnopyranosyl- (1-3) -2,4-di-O-acetyl-aL-rhamnopyranosyl- (1-6) -bD -galactopyranoside] was identified as having a molecular weight of 824, molecular formula C ₃₇ H ₄₄ O ₂₁ .

[Formula 1]

[Formula 2]

[Formula 3]

Examples of the formulation of the composition according to one aspect of the present invention will be described below, but it is also applicable to various other formulations, which are intended to explain in detail only and not intended to limit the present invention.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

According to the composition shown in Table 1 below to prepare a flexible cosmetic water in a conventional manner.

Table 1.

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Nutritional longevity was prepared according to the composition described in Table 2 below in a conventional manner.

Table 2.

[Formulation Example 3] Pack

To prepare a pack in a conventional manner according to the composition described in Table 3.

Table 3.

As described above, specific portions of the present disclosure have been described in detail, and for those skilled in the art, these specific techniques are merely preferred embodiments, and the scope of the present disclosure is not limited thereto. Will be obvious. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

It contains water extract of Actinidia polygama as an active ingredient,
Cosmetic composition for absorbing ultraviolet light B that absorbs ultraviolet light B of 280 to 320 nm.
delete The method of claim 1,
The extract is a leaf, stem, or a mixed extract of the leaves of the fortune, ultraviolet B absorbing cosmetic composition.
delete delete The method of claim 1,
The extract is contained in a concentration of 10 to 10,000 ppm with respect to the composition, ultraviolet B absorbing cosmetic composition. delete

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