KR20050073918A - Cosmetic composition comprising plant extract having anti-aging effect - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed plant extract of Angelica tang, Sukji-hwang, Cheongung, Jigija, Sewao, Astragalus, Higae, Licorice, and Baekjak as an active ingredient, in detail a composition comprising the mixed plant extract of the present invention Has an excellent antioxidant effect, elastase inhibitory effect, lipoxygenase enzyme activity inhibitory effect and wrinkle relief effect can be usefully used as a functional cosmetic composition for preventing and preventing skin aging.

Description

Cosmetic composition containing a mixed plant extract having an anti-aging effect

The present invention relates to a cosmetic composition containing a mixed plant extract of Angelica tang, Sukji-hwang, Cheonggungja, Schisandra, Shou-o, Astragalus, Hyeonggae, Licorice, Baekjak as an active ingredient.

One of the causes of aging is free, caused by free radicals such as superoxide anion radicals, hydroxy radicals, singlet oxygen and hydrogen peroxide (H ₂ O ₂ ) derived from oxygen. Radical reactions destroy major macromolecules of cells, including lipids, which are known to be highly reactive and cause abnormal metabolism by reacting with cellular components or cell membranes. Such abnormal metabolism, exposure to physical and chemical external stimuli can lead to imbalances in oxidation and antioxidants, resulting in peroxidation or incapacity of defense mechanisms in vivo, oxidation and degeneration of proteins, impaired cell function, Eventually, skin aging, characterized by decreased elasticity, wrinkles, blemishes and freckles, is accelerated ( J. Soc. , Cosmet. Sci. Kor. , 23 , pp75-132, 1997). In particular, one of the many causes of aging and disease in living organisms can be found in active oxygen, which occurs during oxidative metabolism, and these are found in healthy tissues by white blood cells. Although it is essential for non-specifically removing non-magnetic substances, it is known to cause peroxidation of lipids by being involved in free radical reactions mainly in biological membranes rich in unsaturated fatty acids. Although there are known active oxygen inhibitory and antioxidant substances used as raw materials for general cosmetics, medicines and foods, this is a synthetic product and its use is limited because it is not secured when applied to human skin for a long time. Natural products are mostly weak in inhibiting free radicals, but the safety of human skin is guaranteed. Therefore, a substance having strong active oxygen inhibitory effect and antioxidant effect and having another effect on human skin is desired.

The most common irritant of skin is ultraviolet light. The action of proteolytic enzymes, such as elastase, released from PMNs (polymorphonuclear leukocytes) by the stimulation of ultraviolet rays, which damage tissues and cells, and excess harmful oxygen species generated during phagocytosis. Inflammation is amplified. In the skin, the elastic fibers, along with the collagen fibers, form crosslinks inside the epidermis. As we age, the elasticity of our skin is drastically reduced by the action of elastinase, an elastin degrading enzyme, and sagging occurs. Histologically, the infiltration of inflammatory cells is increased, the deficiency and aggregation of elastin fibers, the reduction of collagen fibers are shown, and biochemically, the activity of elastase is significantly increased. Elastase is the only enzyme known so far to break down elastin, and its inhibition can fundamentally reduce skin aging.

The mechanism of skin aging is as follows. Free radicals or free radicals are generated by UV rays or external environmental factors. Cell membranes are attacked by the generated free radicals or free radicals, resulting in inflammation of the skin and inflammated tissues. The aging process leads to skin aging that destroys proteins and genetic material and forms wrinkles. Therefore, only inhibiting and preventing all steps involved in the skin aging mechanism can fundamentally prevent skin aging. Due to the disadvantage that the raw material having a conventional anti-aging effect is not fundamentally inhibited, research has been attempted to find new functional materials. Various natural products, which have been conventionally used as herbal medicines, have not only been excellent in safety, but also contain various beneficial drug substances, and have been a good search target.

Angelica acutiloba (S. et Z.) KITAGAWA of the present invention is a naturalized plant that is cultivated in Korea, and is a perennial herb that is native to Japan, and is 60 to 90 cm high, and the flowers are white and end to branch in August to September. Running fruit is long, flat oval with narrow wings at edge. The root is called Angelica, which has the effect of blood, so there is no blood on the face, dizziness is frequent, and it is known to be effective for people who have no gloss on eyes and lips. Headache is weak body, women's menstrual control effect is excellent, so it is often used in various prenatal and postpartum diseases, and it treats menstrual irregularities and abdominal pain. It is effective in treating constipation due to weak gastrointestinal function (Bae Ki-hwan), color guidebook for health guidelines according to folk remedies and herbal remedies, pp102-103, 2003.

Rehmannia glutinosa (GAERTN.) LIBOSCH. Is a perennial medicinal plant accompanying the Scrophulariaceae, which is dried Chinese roots and dried in the roots of a cucurbita. Sukjihwang is sweet and slightly warm, and it is due to catalpol that blacks when dried. In addition, luteolin and chrysoriol (3'-methyl ester of luteolin) were separated from the leaves and roots of the cucurbita. In motion therapy, weak body, back pain symptoms, dysmenorrhea, dizziness, blood donation medicine, tonic medicine, blood poisoning medicine, urinary medicine, and kidney remedy medicine, anemia, disease weakness, hemorrhage, uterine bleeding, tuberculosis Healing the debilitating (Moon et al., Composition and Use of Herbs, Encyclopedia Press, pp656-657, 1999).

Cnidium officinale MAKINO is a perennial herb of the Umbelliferae, native to China and grown as a medicinal crop. The height is 30 to 60 cm, and the components are known as cnidilide, ligustilide, neoocnidilide, butylphtalide, sedananoic acid, and the like. Cheongung has uterine sedation, blood pressure control, antibacterial action, and inhibitory effects on skin reproductive bacteria. It is used for headache, anemia, and gynecological diseases. Metabolism, Yeonglimsa, pp418-419, 1998).

Jilja ( Tribulus terrestris L.) is a fruit of lead bird, also called silly. It is a thorny polygon. The tired person is very tasty and warm. Yellow flowers bloom in summer and grow south of central part. Ingredients include saponin 1.5%, essential oils and alkaloids. There is resin, oil. It causes spear disease, pruritus, vitiligo, and cramps, enters the cirrhosis, not only lowers blood pressure significantly, but also softens blood vessels and dissolves cholesterol to improve blood circulation. Get rid of the wind and make sure you have a good time. In oriental medicine, it is used as an anti-inflammatory medicine to cure itching and to treat eye diseases.

Sewage ( Polygonum multiflorum THUNB.) Is a perennial vine grass with stems growing about 1 to 3 meters and running like beads in the shape of cylinders or scales. It is 5 to 15 centimeters long and 1 to 3.5 centimeters thick. The fruit is light brown in September, and when the fruit ripens, the fruit peels off and the seeds with long white hairs blow in the wind. Hasuo is said to have blackened hair that was white in the old days with a nourishing tonic. Sewage strengthens the kidneys, liver, and heart, lowers blood pressure, prevents cholesterol from accumulating in the liver, strengthens the brain, and replenishes blood. It also strengthens your back and knees and strengthens your fitness. Nervous breakdown, insomnia, forgetfulness, heart palpitations (Jin Kyu Choi, our grass, flowers, trees, 1 , pp242-253, 2001).

Astragalus membranaceus (FISCH.) BGE. Is called Astragalus. The outside is light brown or yellowish brown and the inside is yellowish white with a soft sweet scent. Ingredients include sucrose, glucuronic acid, mucus, amino acid, glutinous substance, choline, betaine, etc., and it is known that the medicinal effect of Astragalus has the effects of cold, diuretic, and liver, and lowers blood pressure. In addition, the New Farming Menarche is particularly effective for the elderly and has a prolonged lifespan, which is called Astragalus (No Seung-hyun, Hwang Puppet, TV Main Herb, p332, 1999; Bae Ki-hwan), Health guidelines based on folk remedies and herbal remedies. Color Book, p445, 2003).

The hedgehog ( Schizonepeta tenuifolia var. Japonica KITAGAWA) is an annual herb, native to China, 60cm high, with its main stems standing upright, square and facing each other. The flowers bloom in August-September, and the outposts with flowers are called hyunggae, which are effective when the fever is cold, cold and shivering, headache, but no sweating, and itching, scabies, eczema, and swelling. Also, it is known that nosebleeds often burst or stop urinary bleeding and uterine bleeding (Bae Ki-hwan, color guidebook for health guidelines according to folk remedies and herbal remedies, p437, 2003).

Licorice ( Glycyrrhiza uralensis FISCH.) Is a perennial herb that is 30 to 80 cm tall. The shell is thin and reddish, straight, long and very sweet. In addition, the curved side should have hard yellow fiber. The main components of licorice include licorice saponin, flavonoids and glycyrrhizin. Roots and rhizomes contain 5-14% glycyrrhizin and glabraic acid, which are very helpful for cough medicine and bronchial asthma. Licorice is also used for gastroduodenal ulcers, jaundice, eczema and other skin diseases. It is known to relieve pain and urgency caused by rapid muscle tension, and has detoxification, antitussive and expectorant effects. Lee Yong, Encyclopedia of Science, pp. 387-393, 1999; Roh Seung-hyun, Hwang Puppet, TV Primrose, p297, 1999).

Paeonia japonica (MAKINO) MIYABE et TAKEDA is a perennial herb that is less than 50cm tall and blooms in May-June. In autumn, care roots and beard roots are removed, washed and dried. Root contains 1.8 ~ 7.3% of paeoniflorin and root bark contains 1 ~ 1.1%. In addition, it contains essential oils, fatty oils, resins, tannins, sugars, and mucus. Paeniflorin of Count is effective in antifungal and menstrual pain in addition to soothing analgesic and anti-inflammatory effects (Bo Bo-seop, Shin Min-gyo, Hyangjeomsa, Younglimsa, pp524-525, 1998)

Recently, in order to develop a relatively toxic and naturally friendly cosmetics, it has been discovered that several plants are effective in preventing skin aging and wrinkles by searching for plants having radical scavenging action. As an example, Korean Patent Publication No. 1994-35688 discloses a radical scavenger containing an extract of three hundred s, and Korean Patent Publication No. 1994-36642 discloses a radical scavenger containing an extract of a plant of the genus Pheasant. , Korean Patent Publication No. 1997-58952 discloses skin wrinkle improvement cosmetics containing Yulpi extract, and Korean Patent Publication No. 2002-44266 discloses anti-aging of skin containing 38 herbal extracts such as Megmun-dong, health and root skin And whitening cheongung, licorice, Schisandra chinensis and Earl cosmetic composition have been disclosed, but until now the skin aging of the composition containing the mixed plant extracts of Angelica, Sukji Hwang, Cheongung, Gigija, Sewao, Astragalus, hyungae, licorice, Earl Prevention and anti-wrinkle action is not known.

Therefore, the present inventors searched for a substance that can prevent skin aging fundamentally by using a substance derived from natural products, and as a result, the mixture of Angelica, Sukjihwang, Cheonggung, Jigija, Sewao, Astragalus, Hyeonggi, Licorice, Baekja The plant extract was confirmed to have an excellent antioxidant effect, elastase inhibitory effect, lipoxygenase enzyme activity inhibitory effect and wrinkle relief effect to prevent skin aging to complete the present invention.

It is an object of the present invention to provide a functional cosmetic composition for preventing and preventing skin aging having excellent antioxidant effect, elastase inhibitory effect, lipoxygenase enzyme activity inhibitory effect and wrinkle relief effect, and having no safety problems.

In order to achieve the above object, the present invention provides a functional cosmetic composition for preventing and preventing skin aging containing a mixed plant extract of Angelica, Sukji Hwang, Cheonggung, Zilja, Sewage, Astragalus, Hyeonggi, Licorice, Baekjak as an active ingredient do.

The above donkey: Sukjiwang: Cheongung: Tired: Sewao: Astragalus: Hundred: Licorice: White Pear: 1-30: 1-10: 1-20: 1: 10: 1-20: 1-15: 1-20: 1 20:30 to 70 dry weight ratio, preferably 1 to 10: 1 to 5: 5 to 10: 1 to 5: 5 to 15: 3 to 10: 1 to 10: 5 to 10:40 to 60, more preferably Preferably 5: 5: 5: 5: 10: 5: 5: 5: 55 can be used for the preparation of extracts, and each extract is 1-30: 1-10: 1-20-20: 1-10: Dry weight ratio of 1-20: 1-15: 1-20: 1-20: 30-70, Preferably 1-10: 1-5: 5-10: 1-5: 5-15: 3-10: 1-10: 5-10: 40-60, More preferably, it can mix and use to 5: 5: 5: 5: 10: 5: 5: 5: 55.

In addition, the mixed plant extracts include those extracted with one or more solvents selected from the group consisting of C ₁ to C ₄ lower alcohols such as water, methanol, ethanol, propylene glycol, butylene glycol, glycerin, and mixed solvents thereof. .

The mixed plant extract is characterized in that it is deposited by 1 to 15 days at 5 to 50 ℃, or heated for 2 to 20 hours at 50 to 100 ℃.

The mixed plant extract is characterized in that it contains 0.001 to 20% by weight, preferably 0.01 to 10% by weight relative to the total weight of the composition.

Hereinafter, the present invention will be described in detail.

The mixed plant extract of the present invention is dried, chopped or pulverized after the complete drying of the Angelica, Sukjihwang, Cheonggung, Jigija, Sewage, Astragalus, hyeonggae, licorice, Baekjak, to each pulverized or 1 ~ 10%, Sukju sulfur 1 ~ 5%, Cheongung 5 ~ 10%, Tired 1 ~ 5%, Sewage 5 ~ 15%, Astragalus 3 ~ 10%, Hunggi 1 ~ 10%, Licorice 5 ~ 10%, Earl 40 ~ 60% In one mixture, about 1 to 30 times the weight (kg), preferably about 5 to 15 times water, C ₁ to C ₄ lower alcohol, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof, Preferably, distilled water is added and extracted using a cold, hot water extraction, ultrasonic extraction, reflux cooling extraction method for about 0.5 hours to 15 days at an extraction temperature of 0 ° C. to 100 ° C., and the extract is thus filtered, concentrated under reduced pressure or Drying can produce the extract.

Preferred extraction method is a cooling condenser is installed in the state to prevent the evaporation of the solvent is 50 to 100 ℃, extracted by heating for 2 to 20 hours or 5 to 50 ℃ for 1 to 15 days to extract the active ingredient The method may be used, and the mixed plant extract thus extracted may be used by filtration or may be concentrated and completely concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser.

The mixed plant extract of the present invention prepared by the above is preferably an extract containing a certain solid content, showed excellent antioxidant and elastase inhibitory effect, and when applied to the skin has an effect of increasing skin elasticity and relieving wrinkles It can be used as a functional cosmetic composition for preventing and preventing skin aging.

The present invention contains a mixed plant extract of Angelica, Sukji Hwang, Cheonggungja, Sigija, Sewao, Astragalus, Higae, Licorice, Baekjak in 0.001-20% by weight, preferably 0.01-10% by weight of the total cosmetic composition According to a conventional method in the present invention provides a cosmetic composition for preventing and preventing skin aging mixed with a cosmetically applicable substrate.

In addition, the cosmetic composition of the present invention is applied and formulated as necessary components, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., which are formulated in general skin cosmetics. It is possible.

The cosmetic composition containing the mixed plant extract of the present invention as an active ingredient may be used in a variety of moisturizing cosmetics, face wash, body cosmetics and the like.

Examples of products to which the present composition can be added include cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and cleansing agents, face washes, soaps, treatments, and essences. .

Specific examples of the cosmetic of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, Shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder, eye shadow and the like.

Hereinafter, the present invention will be described in detail by Examples, Formulation Examples and Experimental Examples.

However, the following Examples, Formulation Examples and Experimental Examples are merely illustrative of the present invention, the contents of the present invention is not limited by the following Examples, formulation examples and experimental examples.

Example 1 Preparation of Mixed Plant Extract

1 kg of dried dried Angelicae 5%, Sukjihwang 5%, Cheongung 5%, Jilja 5%, Sewao 10%, Astragalus 5%, Hyungae 5%, Licorice 5%, Earl 55%, in 10 kg of distilled water Extracted for 3 hours at 100 ℃ in an extractor with a cooling condenser, filtered with a 400 mesh (mesh) filter cloth, cooled to room temperature and left to mature at 5-15 ℃ for 7 days, then filtered with a 0.45 ㎛ filter mixed plant extract 8.7 kg (3.3% solids) were obtained.

Examples 2-4. Preparation of Mixed Plant Extracts

Extracting in the same manner as in Example 1 except for the solvent conditions only to obtain a mixed plant extract of Examples 2 to 4, the yield and solid content are described in Table 1 below.

menstruum Yield (kg) Solid content (%) Example 2 30% hydrous alcohol 8.4 2.8 Example 3 30% water propylene glycol 8.5 2.5 Example 4 30% water butylene glycol 8.8 3.0

Example 5. Preparation of Mixed Plant Extracts

1kg of the dried dried Angelica 5%, Sukjihwang 5%, Cheongung 5%, Jilja 5%, Sewage 10%, Astragalus 5%, Hyungae 5%, Licorice 5%, Earl of 55% in 10 kg of distilled water at room temperature After aging for 7 days, the mixture was filtered through a 400 mesh filter cloth and filtered through a 0.45 μm filter to obtain 8.5 kg of mixed plant extract (2.1% solids).

Examples 6 to 8. Preparation of Mixed Plant Extracts

Extracting the mixed plant extracts of Examples 6 to 8 by the same method as in Example 5 except for the solvent conditions only, the yield and solid content are described in Table 2 below.

menstruum Yield (kg) Solid content (%) Example 6 30% hydrous alcohol 8.4 2.0 Example 7 30% water propylene glycol 8.6 1.8 Example 8 30% water butylene glycol 8.7 3.2

Example 9. Preparation of Angelica Extract

1 kg of completely dried Angelica was added to 10 kg of 30% aqueous butylene glycol, aged for 7 days at room temperature, extracted, filtered through a 400 mesh filter cloth and filtered through a 0.45 μm filter to obtain 8.6 kg (2.9% of solid content) of Angelica Extract. .

Examples 10 to 17. Preparation of extract for each raw material

Extracted in the same manner as in Example 9 using the raw material of Table 3 below to obtain an extract for each raw material.

Raw material Yield (kg) Solid content (%) Example 10 Hungjiwang 8.1 2.6 Example 11 Cheongung 8.3 1.9 Example 12 Tired 8.4 1.6 Example 13 Shouao 8,8 2.5 Example 14 Astragalus 8.3 1.7 Example 15 Brother-in-law 8.4 1.6 Example 16 licorice 8.8 3.3 Example 17 Earl 8.1 2.3

Experimental Example 1. Measurement of antioxidant effect

The superoxide radical scavenging effect of the mixed plant extracts was measured by Wako et al. (Murakami, A. et. Al, Oncology, 53 , pp386-391).

That is, the concentrations of the extracts of Examples 1 to 17 in 2.4 ml of 0.05 M Na ₂ CO ₃ solution, 0.1 ml of 3 mM xanthine (Xanthine, Sigma, x4002), 0.1 ml of 3 mM EDTA and 0.1 ml of 0.75 mM NBT were mixed. 0.1 ml each was added and allowed to stand at 25 ° C. for 10 minutes. Subsequently, 0.1 ml of Xanthine oxidase (Sigma, × 4375) was added and reacted at 25 ° C. for 20 minutes, and then 0.1 ml of 6 mM CuCl ₂ was added to absorb the absorbance at 560 nm of the UV-visible spectrum. Vitamin C (Vitamin C) was used as a control. Peroxide radical scavenging activity SC ₅₀ is the concentration of the mixed plant extract required for 50% scavenging of the peroxide radical, the SC ₅₀ value of the control was 253, SC ₅₀ measurement results for the extracts of Examples 1 to 17 are shown in the following table 4 is shown.

Example SC ₅₀ (μg / ml) Example 1 277 Example 2 310 Example 3 273 Example 4 268 Example 5 400 Example 6 301 Example 7 527 Example 8 263 Example 9 372 Example 10 960 Example 11 532 Example 12 820 Example 13 738 Example 14 > 1,000 Example 15 871 Example 16 > 1,000 Example 17 663

Experimental Example 2. Cell Membrane Protection Experiment

The extracts of Examples 1 to 17 were measured according to the method of Park (Park, S. N, Journal of Korean Society of Cosmetic Products, 23 (1) , pp75-132, 1997).

The red blood cells (white blood cells removed) used in the experiment were collected from Albino rabbits from New Zealand. The red blood cell suspension had an OD of 0.6 at 700 nm and a red blood cell count of 1.5 × 10 ⁷ cells / ml. 50 μl of the extract was added to the red blood cell suspension, pre-incubated for 30 minutes in the dark, and then irradiated with a light sensitizer (rose-bengal). After irradiation for 15 minutes, the degree of destruction of erythrocytes by post-incubation was determined from light transmittance (% transmittance) at 700 nm at 15 minute intervals. The effect of the extract on photohemolysis was expressed as τ ₅₀ indicating post-culture time and degree of hemolysis, and τ ₅₀ value was the time (minutes) required for 50% hemolysis of red blood cells. 1,3-butylene glycol was used as a control, and green tea extract having cell membrane protection was used as a positive control. The τ ₅₀ value of the green tea extract was 320 minutes, and the experimental results are shown in Table 5 below.

Example Membrane protection (τ ₅₀₎ : minute)   Control 20  Example 1 295  Example 2 175  Example 3 285  Example 4 271  Example 5 163  Example 6 236  Example 7 250  Example 8 305  Example 9 265  Example 10 210  Example 11 225  Example 12 255  Example 13 211  Example 14 175  Example 15 225  Example 16 163  Example 17 236

Experimental Example 3. Determination of the effect of elastase inhibitory activity

The inhibitory effect of elastase activity was measured on the extracts of Examples 1 to 17 (Lee et al., J. Cosmetic Science , 49 , pp 285-297, 1998).

That is, 20 μl of substrate solution (elastase substrate Succ-Ala-Ala-Ala-p-nitroanilide standard, 8.8 mM) in 60 μl of a 0.267 M Tris solution adjusted to 0.267 M hydrochloric acid to pH 8.0 and the above Example 100 μl of the sample prepared by diluting each extract of 1 to 17 to a predetermined concentration (0.1%) in purified water was added to the mixture, and 20 μl of the enzyme solution which made the Porcine Pancreatic Elastase standard to 10 μg / ml. After the addition, the mixture was reacted at 25 ° C. for 15 minutes, and the absorbance B was measured at a wavelength of 410 nm for the amount of p-nitroaniline produced. 20 μl of the substrate solution, 20 μl of purified water, and 100 μl of the sample were added to 60 μl of the buffer solution, and the absorbance C was measured using the solution obtained by the same method as the control solution. Separately, the absorbance A was measured using 100 μl of purified water instead of the sample as a blank solution, and the absorbance A was measured using 120 μl purified water instead of the enzyme solution and the sample solution. The absorbance D was measured and the elastase inhibition rate (%) was measured according to the following equation (1). Elastase inhibitory activity IC ₅₀ was expressed as the concentration (μg / ml) of the extract required to inhibit 50% of the substrate of elastase, and the results are shown in Table 6 below.

% Elastase inhibition = [1-(B-C) / (A-D)] X 100

Example IC ₅₀ (μg / ml) Example 1 466 Example 2 525 Example 3 396 Example 4 401 Example 5 443 Example 6 492 Example 7 388 Example 8 351 Example 9 870 Example 10 > 1,000 Example 11 > 1,000 Example 12 576 Example 13 673 Example 14 559 Example 15 733 Example 16 801 Example 17 587

Experimental Example 4. Lipoxygenase Enzyme Activity Inhibitory effect measurement

The inhibitory effect of lipoxygenase enzyme activity on the extracts of Examples 1 to 17 was measured using the TBARS method (Ohkawa et. Al , Anal. Biochem., 95 , p351, 1979).

That is, lipoxygenase was prepared at a concentration of 6,000 units / ml in sodium phosphate buffer containing 1.12 mM linolenic acid, and then 1 ml of linolenic acid solution, 0.9 ml of enzyme solution, and Examples 1 to 17. 100 μl of each extract sample was added and reacted at 25 ° C. for 10 minutes, and 20% trichloroacetic acid and 0.6% TBA (thiobarbituric acid) were added to measure absorbance at 535 nm. At this time, the control group used sodium phosphate buffer instead of the enzyme solution, and each extract of Examples 1 to 17 at the same concentration as the experimental group was added. The results are shown in Table 7 below.

Example Inhibition Rate (%) Example 1 52 Example 2 48 Example 3 45 Example 4 39 Example 5 21 Example 6 44 Example 7 51 Example 8 59 Example 9 46 Example 10 42 Example 11 12 Example 12 33 Example 13 36 Example 14 35 Example 15 27 Example 16 19 Example 17 32

Preparation Example 1

An example of preparation of a lotion in cosmetics containing plant extracts obtained from a mixture of Angelica, Sukji-hwang, Cheonggung, Jigija, Sewao, Astragalus, Higae, Licorice and Baekjak is as follows. The mixed plant extracts used herein are those of Example 8 above.

number Raw material Formulation Example 1 Comparative Formulation Example 1 One   Mixed plant extracts 50.0 - 2 Butylene glycol 7.0 7.0 3 glycerin 5.0 5.0 4 Polyoxyethylene (60) Cured Castor Oil 0.2 0.2 5 ethanol 5.0 5.0 6 Betaine 2.0 2.0 7 Citric acid 0.02 0.02 8 Sodium citrate 0.06 0.06 9 antiseptic a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Remaining amount Remaining amount

<Production method>

Formulation Example 1 and Comparative Formulation Example 1: Raw materials 2, 3, 4, 6, 7, 8, and 9 were added to the purified water of raw material No. 11, stirred to dissolve (A), and then dissolved by adding 10 times to raw material No. 5 This is poured into A and stirred. To this was added 1 and stirred sufficiently before aging.

Preparation Example 2 Lotion

Examples of the preparation of lotions in cosmetics containing plant extracts obtained from the mixtures of Angelica tang, Sukji-hwang, Cheonggung, Jigija, Sewao, Astragalus, Higae, Licorice and Baekjak are as follows. The mixed plant extracts used herein are those of Example 8 above.

number      Raw material Formulation Example 2 Comparative Formulation Example 2 One   Mixed Plant Extract 40.0 - 2 Butylene glycol  8.0  8.0 3 glycerin  5.0  5.0 4 Squalane 10.0 10.0 5 Butylene Glycol Dicaprylate / Dicaprate  5.0  5.0 6 Sorbitan stearate  1.5  1.5 7 Polysorbate 60  1.0  1.0 8 Glyceryl Stearate  0.5  0.5 9 Stearyl glycyrrhetinate  0.2  0.2 10 Carboxy Vinyl Polymer  0.1  0.1 11 Arginine  0.1  0.1 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Production method>

Formulation Example 2 and Comparative Formulation Example 2: Raw materials 1, 2, 3, 10, 11, 12, and 14 were mixed by heating to 70-75 ° C. while stirring (A). Raw materials 4, 5, 6, 7, 8, and 9 were heated to 75 to 80 ° C. while stirring (B), and then added to A to emulsify and then cooled to 45 ° C. while stirring, and 13 times were added thereto. After stirring, the solution is cooled to 30 ° C. and aged.

Preparation Example 3 Essence

An example of preparation of an essence in cosmetics containing a plant extract obtained from a mixture of Angelica, Sukji-hwang, Cheonggung, Jigija, Sewao, Astragalus, Higae, Licorice and Baekjak is as follows. The mixed plant extracts used herein are those of Example 8 above.

number Raw material Formulation Example 3 Comparative Formulation Example 3 One   Mixed plant extracts 10.0 - 2 Cytostolol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Carboxy Vinyl Polymer 0.2 0.2 10 Xanthan Gum 0.2 0.2 11 antiseptic a very small amount a very small amount 12 Spices a very small amount a very small amount 13 Purified water Remaining amount Remaining amount

<Production method>

Formulation Example 3 and Comparative Formulation Example 3: Raw materials 2, 3, 4, 5 and 6 were stirred and mixed (A), and raw materials 1, 7, 8 and 13 were separately stirred and mixed (B). After adding B to emulsification and emulsifying, when it is cooled to about 45 ° C. while stirring, 12 times is added, stirred, cooled to 30 ° C., and aged. The raw materials 9, 10 and 11 are added to disperse to stabilize and mature.

Preparation Example 4 Facial Cleanser (Cleansing Foam)

Examples of preparation of face washes in cosmetics containing plant extracts obtained from a mixture of Angelica, Sukji Hwang, Cheonghung, Cheonggija, Sewao, Astragalus, Higae, Licorice and Baekjak are as follows. The mixed plant extracts used herein are those of Example 8 above.

number      Raw material Formulation Example 4 Comparative Formulation Example 4 One  Mixed Plant Extract 20.0 - 2  N-acyl glutamate 20.0 20.0 3  glycerin 10.0 10.0 4  PEG-400 15.0 15.0 5  Propylene glycol 10.0 10.0 6  POE (15) oleyl alcohol ether 3.0 3.0 7  Lauric derivatives 2.0 2.0 8  Methyl paraben 0.2 0.2 9  EDTA-4Na 0.03 0.03 11  Spices 0.2 0.2 12  Purified water Remaining amount Remaining amount

<Production method>

Formulation Example 4 and Comparative Formulation Example 4 After mixing 3, 4, and 5 times with raw material No. 12, 1 time is added in small portions. Heat and dissolve at 80 ° C. while stirring 9 times (A), and heat 6 and 7, 8 (B) separately at 80 ° C., dissolve and add to A, stir and mix, and then add 11 times. Cool slowly.

Preparation Example 5 Shampoo

Examples of the preparation of shampoos in cosmetics containing plant extracts obtained from the mixtures of Angelica tang, Sukji-hwang, Cheonggung, Jigija, Sewao, Astragalus, Higae, Licorice and Baekjak are as follows. The mixed plant extracts used herein are those of Example 8 above.

number      Raw material Formulation Example 4 Comparative Formulation Example 4 One  Mixed plant extracts 20.0 - 2  TEA Lauryl Sulfate 20.0 20.0 3  Sodium Lauryl Ether Sulfate 30.0 30.0 4  Lauryl Amidopropyl Betaine 2.0 2.0 5  Lauryl acid diethanol amide 3.0 3.0 6  glycerin 2.0 52.0 7  EDTA-2Na 0.05 0.05 8  Methyl paraben 0.2 0.2 9  Citric acid 0.03 0.03 10  Zinc pyrithione 0.02 0.02 11  Spices 0.2 0.2 12  Purified water Remaining amount Remaining amount

<Production method>

Formulation Example 4 and Comparative Formulation Example 4: 6, 7 times were mixed with raw material No. 12, heated and dissolved at 80 ° C., and 2, 3, 4, 5, 6 times were added and stirred. After checking dissolution, neutralize by adding 9 times at 50 ° C, and then stirring and mixing 1 and 10 times at 45 ° C.

Test Example 1 Anti-Aging Confirmation Experiment on Skin

1-1. Increased elasticity test on the skin

In order to measure the effect of increasing the elasticity of the skin when the lotion of Formulation Example 2 and Comparative Formulation Example 2 of Preparation Example 2 is applied to the skin, a female layer of 20 to 55 years of age, normal, oily, dry, complex skin 20 owners were asked to apply the lotion of Formulation Example 2 on the left side of the face and the lotion of Comparative Formulation Example 2 on the right side of the face twice a day, morning and evening, followed by 1, 2, 3 and 4 weeks. After weeks, the elasticity of the skin was measured using a skin elasticity analyzer (Ballistometer). The measurement results are shown in Table 13 below.

Cosmetic formulation of Formulation Example 2 Cosmetics of Comparative Formulation Example 2 Remarks T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄ Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 amplitude first 1.9 4.8 5.0 5.1 5.6 1.9 3.0 3.3 3.6 3.6 second 1.3 4.6 5.2 5.2 5.8 1.6 2.0 2.3 2.5 2.9 third 0.0 2.4 3.6 3.7 3.7 0.0 0.0 1.9 2.5 2.5 fourth 0.0 1.0 2.9 3.3 3.5 0.0 0.0 0.0 0.0 0.0 Average 0.80 3.20 4.17 4.33 4.65 0.88 1.25 1.88 2.15 2.25

T ₀ ; Before use

T ₁ ; After 1 week

T ₂ ; After 2 weeks of use

T ₃ ; After 3 weeks of use

T ₄ ; After 4 weeks of use

As can be seen from the above results, compared to the lotion of Comparative Example 2 prepared without the lotion of Formulation Example 2 containing the mixed plant extract according to the present invention, the skin elasticity effect by the skin condition improvement effect is very It was confirmed that excellent.

1-2. Elasticity improvement effect test by measuring wrinkle depth

To test the effect of mixed plant extracts on skin elasticity, 20 females aged 20 to 55 years were compared with the essence of Formulation Example 3 at the left eye edge compared to the right eye edge to 20 owners of normal, oily, dry and combination skin. Essence of Formulation Example 3 was to be applied twice a day. After 14 days of each treatment, the skin elasticity of the skin surface was measured using a cutometer SEM 474. Skin elasticity measurement by the cutometer SEM 474 is a measure of the depth of wrinkles, the smaller the value is evaluated to be good elasticity, the elasticity value is expressed as a percentage decrease compared to the control. The average value of 20 subjects is shown in Table 14 below, and the control group shows the measured value before the sample is treated.

sample Skin elasticity (wrinkle depth reduction) effect (%) 15th 30 days Formulation Example 3 6.3 7.8 Comparative Formulation Example 3 3.8 4.5

As described above, the functional cosmetic composition using the mixed plant extracts of Angelica, Sukjiwang, Cheongung, Cheunggi, Sewage, Astragalus, Hyeonggi, Licorice, and Baekjak of the present invention have excellent antioxidant effect, elastase inhibitory effect, and lipoxygenase. By having an enzyme activity inhibitory effect it can fundamentally prevent skin aging, there is no problem in safety and can be usefully used as a functional cosmetic for preventing and preventing skin aging.

1 is a view showing the skin elasticity improvement effect by reducing the skin wrinkle depth of the essence containing the mixed plant extract of the present invention.

Claims (7)

Functional cosmetic composition for preventing and preventing skin aging, which contains a mixed plant extract of Angelica tang, Sukji-hwang, Cheonggung, Jigija, Sewao, Astragalus, hedgehog, licorice and Earl as active ingredients. The method according to claim 1, wherein the donkey: Sukkuji: Cheongung: Tired: Sewao: Astragalus: Hundred: Licorice: White peony is 1-30: 1-10: 1-20: 1-10: 1-20: 1-15: 1 Cosmetic composition which is a dry weight ratio of -20: 1-20: 30-70. The method according to claim 2, wherein the donkey: Sukjihwang: Cheongung: Tired: Sewao: Astragalus: Hundred: Licorice: White Peony: 1-10: 1-5: 5-10: 1-5: 5-15: 3-10: 1 Cosmetic composition which is a dry weight ratio of -10: 5-10: 40-60. The cosmetic composition according to claim 1, wherein the extract is extracted with water, C ₁ to C ₄ lower alcohols, butylene glycol, propylene glycol, glycerin, or a mixed solvent thereof. The cosmetic composition according to claim 1, wherein the extract is extracted by dipping 1 to 15 days at 5 to 50 ° C, or by heating at 50 to 100 ° C for 2 to 20 hours. The cosmetic composition according to claim 1, wherein the mixed plant extract is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition. The composition of claim 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap Cosmetic composition, characterized in that it has at least one formulation selected from the group consisting of shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.