KR101220850B1 - Composition of cosmetic crude drug compositeness and anti-bacterial activity - Google Patents

Abstract

The present invention relates to a herbal medicine composite cosmetic composition having an antioxidant effect and an antimicrobial effect, and comprises seven herbal medicines of Salvia Militiorrhiza, Angelica, Scrub, Cheongung, Sukjiwang, Baekjak, and Golden, and the herbal medicine composite cosmetic composition extracted with hot water or ethanol as the herbal medicine More specifically, the present invention relates to a herbal medicine composite cosmetic composition having an antioxidant effect and an antimicrobial effect with the natural extract of 0.1 to 40wt% in a conventional cosmetic composition.

The present invention is a herbal composition cosmetic composition with excellent antioxidant effect is a cosmetic composition that can give antioxidant effect, antibacterial effect, moisturizing effect to the skin by using a natural extract having double antioxidant effect and excellent skin affinity and skin compatibility. .

The present invention has an excellent effect on electron donating ability, SOD-like activity, xanthine oxidase inhibitory effect.

Antioxidant, cosmetics, electron donating ability, SOD-like activity

Description

Composition of cosmetic crude drug compositeness and anti-bacterial activity with antioxidant and antimicrobial effects

The present invention relates to a herbal medicine composite cosmetic composition having an antioxidant effect and an antimicrobial effect, and more specifically, a herbal medicine composite material (aka: DanSam Complex) using only seven herbal medicines of Salvia, Angelica, Scrub, Cheongung, Sukjiwang, Baekjak, and Gold. )) The extract is extracted, and the cosmetic composition containing the herbal compound extract is to be given to the skin, such as antioxidant properties, antimicrobial effect excellent skin compatibility, excellent skin compatibility.

Antioxidants are added into the lipid system to prevent the formation of various undesirable compounds due to oxidation.

Various oxidation products produced by oxidation are known to be related to human aging by damaging DNA or causing cancer.

Butylated hydroxy anisole (BHA) and butylated hydroxy toluene (BHT), which are widely used as synthetic phenolic antioxidants, have been widely used because of their effectiveness, economics, and safety. It is known to cause serious toxic effects on the gastrointestinal mucosa, lungs, kidneys, circulatory system, etc., and therefore, development of safe alternative antioxidants is required.

Therefore, researches on natural antioxidants that are harmless to humans and excellent in antioxidant power have been conducted for a long time, and most of the natural antioxidants reported so far are plant-derived.

The antioxidant compounds of most plants are primarily polyphenols, and natural antioxidants have some of their primary activity, but their primary function is their free redical scavenger.

Therefore, natural antioxidants can be developed by searching for redical scavenging function from plant extracts.

The scientific name of Salvia is Saliva miltiorrhiza Bunge, a herb made from root and used in Korea and China.

The characteristics of Dansam (Leeaceae, generic name red ginseng, sheep oil, red muscle, hemoglobin) help blood circulation, remove fish blood, relieve limb joint pain, menstrual irregularity, menstrual pain, postpartum abdominal pain, motility abdominal pain, Treat bruises, etc.

Heals mental fever, bullshit, annoyance, insomnia, skin rash, palpitations due to high fever.

In the field of cosmetics, it has the effect of preventing skin aging by the antioxidant and skin regeneration effect, and it keeps the skin transparent and shiny by smoothly supplying nourishment to the skin.

It clears blood and strengthens blood vessels, thus preventing skin problems.
The scientific name of Angelica is Angelica gigas Nakai, and remove the fibrous roots collected in late autumn and the umbel a perennial herbaceous plants belonging to dry, wait a bit when the water evaporates slowly fumigation smoke.
Angelica is used as a medicine for women's diseases. It has pain, sedation, antiarrhythmia, antithrombosis, blood circulation, blood circulation, tonic, blood pressure strengthening, antiviral and antibacterial effects. It can be kept clean, and it has an excellent effect of inhibiting tyrosinase activity and makes skin clear and elastic.
Antioxidant It absorbs UV rays and prevents skin aging and maintains skin elasticity.
The scrubber 's scientific name is Lufa cylindrica, and it is classified as a perennial vine plant of the dicotyledonous plant, the herbaceous gourd, and its distribution is distributed in Korea, Japan, and China, originating in tropical Asia. (天 羅), loofah cucumber, also called guaru.
It functions to regulate water metabolism and intracellular fluids, and has excellent water retention, which serves to transport and absorb nutrients through water in the body.
Excellent beauty work besides scrubber is also recorded in Lee Si-jin's Herb Herb.
According to this record, healed blood vessels other than loofah and healed meridians to treat blood loss.
It nourishes the skin smoothly and keeps it clear and shiny.

Is the scientific name of the firmament is Cnidium officinale, is a perennial plant that belongs to the oxidation, the rhizome in the hope that dry and used as a medicinal uses that were collected from September to November, water action, hypotensive action, vasodilator action, It has been reported to have antimicrobial, antifungal and pharmacological effects such as vitamin E deficiency treatment.
Suk Ji Hwang 's scientific name is Steamed Rehmannia Root, which is a perennial plant belonging to Hyunsam family and has high potency in immune action and anti-aging such as remedy, hemostatic drug, hemostasis, constipation, libido and diabetes.
Efficacy effect has a soothing effect that makes skin wounds and troubles safe, and has an excellent antibacterial effect and gives skin a smooth and soft feeling.
It also has antioxidant and moisturizing effects.

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The scientific name of Paeonia japonica is Paeonia japonica (Makino) Miyabe & Takeda, and will dry the root of herbaceous perennial of jeokjakyak or dongsok closely related plants that belong to minariah swallow there is antispasmodic, pain, headache, anti-bacterial and anti-thrombotic action are used as therapeutic medicines, and the main component It contains peaoniflorin, benzoic acid, essential oil, fatty acid, tennin, sugar, triterpenoid and β-sitosterol.

Its excellent anti-oxidant action inhibits and delays the skin's aging effect and gives the skin a refreshing feeling and safety through excellent astringent action.

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The scientific name of gold and Scutellaria baicalensis Georgi, belongs to the Lamiaceae origin of the Nago nation everywhere in the Asian continent of origin in northern China, and is geographically distributed in East Siberia, Mongolia, China, Manchuria.

Buribaricin 4-5%, bicarrain, wogonin, wogonoside, 7-methoxy-baikalne, neobicalane, 7-methoxynorwogonin, oroxylon A, school capflavone, etc. .

In addition, there are pyrocatechin-based tannins, beta sitosterol, resins, and essential oils.

The leaves include wogonoside, cartamidine, and isocartamidine.

Therefore, due to the rich Baikal, Baikallein shows an excellent active oxygen scavenging ability, there is an effect to prevent skin aging due to ultraviolet rays.

It is excellent for skin whitening effect and prevents skin damage by sedating skin, excellent anti-inflammatory, antibacterial effect, tyrosinase activity inhibitory effect, and sun protection.

As such, many studies have been conducted on substances having excellent antioxidant and other effects, but it is difficult to make safety such as discoloration, discoloration, pH, viscosity of formulation, and sunlight, which are disadvantages of natural materials, and it is practical to increase the content of additives. There is a difficult point to reap.

The present invention has been provided to efficiently solve the problems of the prior art as described above as a basic experiment of the present invention to explore the cosmetic natural material with excellent antioxidant effect as aloe, yugeunpi, black soybeans, gamguk, white weight, wormwood, cheonmundong, Golden, machi, Eoseongcho, Dansam, Ganoderma lucidum, San Ewha, Pogongyoung, Yulpi, Camellia oil, White peony, Phytoncide, Sukjiwang, Willow, Birch, Potato, Licorice, Red ginseng, Brown seaweed, Guar gum, Cheongung, Sangbaekpi, Sage, Loofah Natural and herbal ingredients such as irises, gardenia, rhododendron, and Angelica were selected to complete the herbal composition cosmetic composition under optimal conditions. Antioxidant experiments were applied to the cosmetics, resulting in a cosmetic composition with excellent antioxidant and antibacterial properties. .

1 is a manufacturing process diagram shown in the present invention.

Extracts of the herbal medicine complex extract by using hot water, ethanol, etc., using seven kinds of herbal medicines such as sweet ginseng, Angelica, loofah, cheongguk, sukjihwang, earl, and gold, to prepare a cosmetic composition containing the herbal medicine complex.

The extract of the herbal medicine of the present invention shows an excellent effect on the antioxidant, such as electron donating ability, SOD (Superoxide dismutase) -like activity, xanthine oxidase inhibitory effect, and is used as a natural antioxidant by showing safe and non-irritating properties compared to synthetic materials It is possible to obtain an excellent antioxidant cosmetics when added to cosmetics.

In addition, it shows growth inhibitory activity against skin flora, and when added to skin aging and atopy-related products, it removes oxidative and dermatological bacteria, one of the causes of skin aging and atopic dermatitis, to provide effective and innovative antioxidant and antimicrobial cosmetic composition. Can be.

The present invention provides a cosmetic composition containing an extract having an antioxidant activity to achieve the above object by extracting from seven leaves, root, fruit, flower part of Salvia, Angelica, Scrub, Cheongung, Sukjihwang, Baekjak, Golden Include.

Extraction solvents include the use of hot water, ethanol and the like.

In addition, the cosmetic composition of the present study includes the basic cosmetic lotion, skin, lotion, cream, pack and hair products such as shampoo, rinse, toner, spray and the like.

After drying the seven herbal medicines of the present invention sufficiently and finely pulverized, a volume of hot water and ethanol of 1 to 50 times, preferably about 1 to 20 times, of the sample is used as a solvent, and preferably 70 to 100% Reflux extraction with ethanol at an extraction temperature of about 10 ° C. to 100 ° C., preferably 50 ° C. to 90 ° C. for about 3 hours to 5 days, preferably 12 hours to 3 days, or 1 to 7 days at 10 ° C. to 40 ° C. After extracting by dipping for 1 day, preferably 1 to 3 days, the extract was cooled and then filtered, and the filtrate was concentrated using a rotary vacuum concentrator, and then lyophilized to prepare a herbal composition having excellent antioxidant composition.

The herbal compound extract of the present invention is a cosmetic composition constituting 0.1wt% to 40wt%, preferably 1wt% to 20wt% based on the total weight of the composition.

If the herbal compound extract is less than 0.01wt%, there is almost no antioxidant effect, and if it is more than 20wt%, it cannot impose cosmetic taste due to the color of the herbal compound cosmetic composition itself. It has the disadvantage of changing.

In order to measure the antioxidant effect of the extract of the herbal medicine complex of the present invention, the electron donating ability, SOD-like activity (Superoxide Dismutase-like Activity), Xanthineoxidase inhibitory effect was confirmed, and used in various cosmetics, hair products and color products for the antioxidant effect Can be.

Herbal compound extracts can be added to the basic cosmetics such as skin lotion, skin, lotion, cream, packs and hair products such as shampoo, rinse, toner, spray and the like.

As described above, the embodiment of the present invention describes a part of the present invention in more detail, but the content of the present invention is not limited thereto.

Example 1. Manufacture of herbal medicine composite extract

end. Hot water extract  Produce

Completely dry seven kinds of herbal medicines of Salvia, Angelica, Scrubber, Cheonghwang, Sukjiwang, Baekjak and Golden, and finely pulverize, 100g of mixed herbal medicine mixture was put in 1,000kg of purified water, boiled in an extractor with cooling condenser and filtered through 300mesh filter cloth. After repeating three times, the mixture was cooled to room temperature, filtered, and the filtrate was concentrated using a rotary depressurizer, and then freeze-dried to obtain an extract of 40 g (hereinafter referred to as 'DCW').

I. Ethanol Extract Preparation

After drying completely, finely pulverized and homogenously mixed 100 g of the herbal mixture was put in 1,000 kg of 70% ethanol, extracted by immersion for 3 days at room temperature, filtered through 300 mesh filter cloth, and repeated three times. After freeze-drying it can be obtained 42g of extract (hereinafter referred to as 'DCE').

Example 2 . Electron donating ability  exam

Example 1 Measurement of the electron donating ability of the sample was performed for DPPH (1, 1-Diphenyl-2-pycryl-hydrazyl), a relatively safe free radical of each extract according to the method of Blois (Blois, MS Nature 181, 1190 (1958)). The hydrogen donating effect was measured.

1 ml of 2 * 10⁴M DPPH (Dissloved in 99% ethanol) was added to 2 ml of the sample at a constant concentration, and the mixture was reacted at 37 ° C. for 30 minutes by vortex mixing for about 10 seconds.

The reaction solution was measured for absorbance at 517 nm using an absorption spectrophotometer.

The electron donating ability effect expressed the difference in absorbance before and after sample addition as a percentage.

Table 1 Electron donating ability  It shows the measurement result. Electron donating ability  You can see that the ability is high.

Vit   C; vitamine

DCW  : Water extrats from Dansam - Complex

DCW  : ethanol extract from Dansam - Complex

Date Shown are mean Values with bars indication the SD of  mean (n = 3)

Example 3 . SOD Similar activity

Example 1 SOD-like activity of the sample was measured by measuring the amount of pyrogallol that catalyzes the conversion of hydrogen peroxide (H 2 O 2) according to Marklund's method.

To 0.2ml of sample, add 3mld of tris-HCI buffer (50mM tris (hydrozymethyl) amino-methane + 10mM EDTA, pH8.5) and 0.2ml of 7.2mM pyrogallol adjusted to pH8.5 for 10 minutes at 25 ℃ Thereafter, 1 ml of 1N HCI was added to stop the reaction.

The amount of oxidized pyrogallol in the reaction solution was measured at 420 nm using a UV / Vis spectrophotometer.

SOD-like activity was expressed as a percentage (%) of the difference in absorbance between the addition and no addition of the sample solution.

Table 2 SOD Quasi-active  It shows the measurement result. SOD  The effect of similar activity can be confirmed.

Vit   C; vitamine

DCW  : Water extrats from Dansam - Complex

DCW  : ethanol extract from Dansam - Complex

Date Shown are mean Values with bars indication the SD of mean (n = 3)

Example 4 . Xanthine oxidase ( Xanthine oxidase Inhibition activity

100 μl of sample and 200 μl of substrate solution in 600 μl of 2 mM Xanthine in 0.1 M phosphate buffer pH7.5 were added and then xanthine oxidase (0.2 u / ml, SIGMA, USA) 100 μl was added.

Xanthine oxidase activity was measured for absorbance at 295 nm of uric acid (uric acid) produced in response to xanthine at 307 ℃ for 15 minutes.

Table 3 shows the results of measuring xanthine oxidase inhibitory activity. The excellent xanthine oxidase inhibitory activity of the herbal compound can be confirmed.

Vit   C; vitamine

DCW  : Water extrats from Dansam - Complex

DCW  : ethanol extract from Dansam - Complex

Date Shown are mean Values with bars indication the SD of mean (n = 3)

Example 5 . How to measure Clear Zone  Antibacterial activity

Staphylococcus aureus, an oral bacterium, Staphylococcus epidermidis, and Escherichia coli, Escherichia coli, were cultured for the antimicrobial effect. .

All strains were incubated at 37 ° C. in a BOD incubator.

Antibacterial activity was measured by a paper disc method.

Incubation was performed at 35 ° C. for 18-24 hours to determine the diameter of the clear zone (mm) around the disc.

As shown in Table 4, the hydrothermal extracts against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli strain did not show antimicrobial effect, and ethanol extract. The antimicrobial effect of 20ug / disc at 17.3 ± 0.1, 22.0 ± 0.2, 18.7 ± 0.1mm, respectively, showing very high activity, the ethanol extract of the herbal compound of the present invention can confirm the excellent antimicrobial effect.

Example 6. Experiment of hair growth promoting effect using mouse

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1) Experimental animals

Twenty-five male 6-week-old males were purchased from the Hard Science Co., Ltd. and used for experiments after adapting to breeding conditions for one week.

The maintenance and management of the experimental animals until the test period were maintained under optimal conditions in the independent animal laboratory.

2) hair growth measurement

Carefully remove hair from the back of the animal using an animal clipper one day before the start of the experiment, and extract 0.2 ml of the herbal compound extract once daily to the hair removal site of five groups of animals (5 animals / group) from the start of the experiment. Apply using a daily brush.

The same coating was applied using 2% minoxyl as a control.

The measurement was carried out daily and observed the hair growth state, the hair growth length was determined at 30 days.

상기 실험에서 Fig5 및 표5에서와 같이 본원발명의 생약복합재 추출물은 항산화 및 항균효과가 탁월하여 모발 성장효과가 높게 나타나는 효과가 있음을 확인할 수 있다.

In the experiments, as shown in Fig. 5 and Table 5, the herbal extracts of the present invention can be confirmed that the hair growth effect is high due to the excellent antioxidant and antibacterial effects.

Example 7. Skins manufacturer

Example 8. Lotion manufacturers

Example 9. Cream manufacturing

실시예10. 샴푸 제조

Example 10. Shampoo manufacturers

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Example 11. Stimulation test

Example 5, 6, 7, 8, 9 was used to perform a skin irritation test.

For skin stimulation test, Waggoner et al., Matsumura et al., Aberer et al. Apply patch body and skin absorbing device to the skin inside the arm and apply it for 48 hours and then check the skin reaction visually. The judgment was made based on the judgment of the contact dermatitis study group (Table 5).

The experiment was conducted on 20 men and women in their 20s and 30s.

As a result of the experiment, the dermatological problems such as redness, mixing, and itching did not occur among 20 volunteers.

1 is a manufacturing process diagram of the present invention

Claims (3)

Herbal composite composition with antioxidant and antimicrobial effects extracted from the group consisting of hot water or ethanol and mixtures of seven herbal medicines of sweet ginseng, Angelica, scrubber, cheonggung, sukjiwang, earl, and gold delete delete

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