KR101930409B1

KR101930409B1 - Detergen composition for prevention of hair loss comprising iceplant as active ingredient

Info

Publication number: KR101930409B1
Application number: KR1020150128409A
Authority: KR
Inventors: 김예주
Original assignee: 김예주
Priority date: 2015-09-10
Filing date: 2015-09-10
Publication date: 2018-12-18
Also published as: KR20170030907A

Abstract

The present invention relates to a method for improving acne skin, cleansing of vulva, cooling, itching, odor removal, and hair loss by including any one selected from the group consisting of ice plant powder, ice plant extract, ice plant juice, , Anti-dandruff and itchy scalp.

Description

[0001] The present invention relates to a detergent composition for preventing hair loss,

The present invention relates to a detergent composition, and more particularly, to a detergent composition, which further comprises an ice plant as an active ingredient to provide a safe, anti-inflammatory, antibacterial, and antifungal effect without cytotoxicity and is effective for improving skin troubles caused by acne, It is possible to use it as a cleanser for women and is excellent in hair loss prevention, dandruff treatment and dandruff prevention effect.

In general, sebaceous glands in the skin, including the scalp and face, play a role in preventing moisturization and microbial invasion of the skin. However, excessive secretion of sebum promotes hair loss, worsens acne, promotes enlargement of pores caused by acne, And many other diseases are caused.

The hypersecretion of sebaceous glands is caused by various causes, and the most important one is the activation of sebaceous glands by the amount of dihydrotestosterone (DHT), which is one of the hormones involved in promoting sebaceous secretion in the activity of sebaceous glands, Is overdone. That is, testosterone (T) is converted into dihydrotestosterone, one of the male hormones, by 5-alpha-reductase type 2 in cells in the hair loss, (5-α-reductase type 1) in the skin or the sebaceous glands. The testosterone is converted to dihydrotestosterone by the action of 5-α-reductase type 1, (J. Invest Dermatol 105: 209-214 Diane et al.).

Acne and hair loss, which are skin troubles other than simple sebum hypersecretion, are further exacerbated by the minute inflammatory reaction of the skin. If you look at the development of acne, excess sebum accumulates in the hair follicle, activating acne bacteria and causing inflammation.

It has excellent antioxidative effect and anti - inflammatory effect. It inhibits the production of prostaglandin, especially prostaglandin E2 (PGE2), and inhibits the production of nitric oxide (NO) by iNOS. The same skin trouble can be alleviated.

However, as a skin-safe natural material, there are not many raw materials that can reduce sebum secretion through fundamental causes, and at the same time, relieve inflammation.

Women's vagina may also have some distinctive odors and secretions even under normal physiological conditions. However, when infected by bacteria from the outside, symptoms such as secretions, itching, and smell appear, and it is said to be the reason why it started to prevent the inflammation caused by the inadequate hygiene control and not to cause discomfort due to excessive body odor There is.

It has a good effect in relieving the causes of various women's diseases such as cervical intraepithelial neoplasia, uterine myoma, uterine cancer, as well as inflammation and itching.

From time immemorial, women have turned their backs for the purpose of treating the vaginosis and vaginitis in the vagina. Most of the materials used are wormwood and leaves, and these materials are used to make a bathing bath.

The vaginal pH of the female is maintained at pH 3.2 by the lactic acid secretion by the beneficial microorganism, and at the low acidity, the vaginal acidity regulator of pH 3.5, which is mainly composed of lactic acid, Its efficacy is limited.

Vaginitis is a female disease that a woman experiences once in a lifetime, and the vagina inside the vagina escapes the normal acidity category, resulting in the proliferation of bacteria. The cause of vaginitis is Trichomonas which cause itching, or Candida which causes a sudden and large amount of vaginal discharge.

Therefore, when used with soap, the vagina can be changed to alkaline to increase the risk of infection, rather than the environment where normal bacteria can not be consumed. Allergy can be caused by the irritant component of alkali.

Therefore, there is a need for a vaginal exclusive detergent which can alleviate allergies or side effects of a low-stimulant pH balance while maintaining a pH balance in the vagina, and capable of suppressing and killing harmful bacteria and alleviating irritation.

Also, dandruff is a keratinized mass formed by the dead or peeled cells of the scalp caused by the active metabolism of the human body, which are dried together with the secretions of the scalp sebaceous glands. Dandruff gradually has a clear boundary with the normal skin, and becomes reddish to increase the scar on the scalp, and it usually goes to the dermatitis with itching and inflammation.

The fundamental cause of such dandruff has not yet been clarified. However, it is generally considered to be caused by imbalance of endocrine hormone, imbalance of nutritional metabolism, neural factors such as stress, biochemical change of epithelial tissue due to excessive proliferation of scalp cells, The growth of microorganisms and the increase of activity, and the inflammation due to air pollution.

In particular, the proliferation and activity increase of microorganisms called Pityrosporum Ovalae, which are present in the unclean scalp, have been recognized for a long time as a main factor for aggravating dandruff and itching, and they have been widely accepted in recent years.

Therefore, the currently used dandruff treatment agent is focused on effectively inhibiting the proliferation and activity of the phytolosformovalle, which is a major cause of dandruff, and normalizing the abnormal metabolism activity of the scalp cell. On the other hand, selenium sulfide and zinc pyrithione are examples of components mainly used in a commercially available anti-dandruff composition.

Although studies on methods and components for preventing or treating dandruff have been continuing, studies on the components that are eco-friendly, harmless to the environment and humans, and capable of reducing the number of dandruff germs are still insufficient . In addition, components that can prevent hair loss while preventing dandruff are not yet known.

1. Korean Patent Publication No. 10-2006-0036290 2. Korean Patent Publication No. 10-2007-0105146 3. Korean Patent Publication No. 10-2008-0109440 4. Korean Patent Publication No. 10-2008-0108706

Accordingly, it is an object of the present invention to provide a method of controlling sebum and an antiinflammatory effect, that is, an anti-inflammatory effect containing a natural material as an active ingredient, by inhibiting the production of an inflammatory factor and thereby improving skin troubles including acne, The present invention is to provide a detergent composition which is suitable for use in women's cleansers and has excellent antimicrobial activity against dandruff, so that the dandruff treatment and anti-dandruff effect are excellent, thereby having a hair loss prevention effect.

In the detergent composition according to the present invention as described above, it is preferable that any one selected from the group consisting of the ice plant powder, the ice plant extract, the ice plant juice, and the mixture thereof is manufactured from a bud.

In the detergent composition according to the present invention as described above, any one selected from the group consisting of the ice plant powder, the ice plant extract, the ice plant juice, and the mixture thereof may be added in an amount of 0.5 to 15 wt. % Can be included.

According to the present invention as described above, it is possible to improve the sebum control effect and the anti-inflammatory effect, that is, to suppress the production of inflammatory factors, thereby improving the skin troubles including acne, The present invention provides a detergent composition which is suitable for use in a female cleanser and is excellent in antimicrobial activity against dandruff, thereby having excellent anti-dandruff effect and dandruff prevention effect and having a hair loss prevention effect.

The present invention provides a safe, anti-inflammatory, antibacterial and antifungal effect without cytotoxicity according to the present invention, and is excellent in improving acne skin, A detergent composition having excellent anti-hair loss, dandruff treatment and anti-dandruff effect The anti-hair loss and anti-dandruff skin external composition contains an ice plant as an active ingredient.

The iced plant according to the present invention is Mesembryanthemum crystallinum , a succulent plant having a low germination rate and a slow growth rate. It is originated in the Namib Desert of South Africa and its main distribution region is South Africa, East West, North, Australia It is the southwest, the arid region of the west of America.

Blister cells, which are crystals similar to ice crystals on stem and leaf surfaces, contain useful components and various minerals such as inositols and beta carotene. Especially, in the ice plant, pinitol, which lowers blood glucose level, (Myoinosito) that inhibits the growth of leaves and stems, or used as a juice diet is suitable for people with diabetes healing food (Healing food vegetable) is.

In Japan, it has been cultivated for many years and processed and utilized as edible and cosmetics. But in Korea, it is an unfamiliar vegetable and it is cultivated and distributed in only a few areas. The present inventor started to study the cultivation of ice plant in 2014 and cultivated in 2015 And succeeded to the present invention through various studies thereafter.

The present invention is characterized in that the ice plant is incorporated into the detergent composition as an active ingredient in various forms of powder, juice, extract, and mixture thereof. In the ice plant, However, according to the various test results of the present inventor, it is most effective to use a bud that comes up before the flower blooms.

The detergent composition according to the present invention preferably contains the ice plant in an amount of 0.5 to 15% by weight based on the total weight of the composition. If the content of the active ingredient is less than 0.5% by weight, the effect or effect is insufficient. If the content is more than 15% by weight, there is a problem in formulation stability.

In addition to the above ice plant, the present invention may further comprise a hot-water extract of liquorice, which is a roots of Disocorea japonica or Disocorea batatas, a perennial herbaceous perennial herb belonging to the family Disocoreaceae as an active ingredient, The acid hydrothermal extract is preferably contained in an amount of 0.5 to 15% by weight based on the total weight of the composition. If the content of the active ingredient is less than 0.5% by weight, the effect or effect is insufficient. If the content is more than 15% by weight, there is a problem in formulation stability.

The detergent composition according to the present invention is not particularly limited in formulation, but may be a composition having a formulation such as solid soap, liquid soap, hair rinse, hair shampoo, and the like. The composition of each of these formulations may contain various components that are formulated into a conventional composition according to the various formulations described above or suitable for the final purpose, and the kind and amount of these components may be readily selected by those skilled in the art.

Hereinafter, the composition according to the present invention will be described with respect to representative base components and additives, taking as an example a representative soap composition and a shampoo composition of the composition.

For example, in the case of a soap composition, in addition to a conventional soap base, it further includes a moisturizing agent, an antioxidant, a light shielding agent, a detergent, and purified water. The moisturizing agent is used for maintaining moisture and supplying various nutrients to the skin and hair after use, and a humectant that can be used in this field can be usually used. Preferred are glycerin, propylene glycol (PG), buthylene glycol (BG), hexylene glycol (HG), urea, dexpanthenol, isopentyldiol, A mixture of one or more selected from the group consisting of pyrrolidone carboxylic acid and its salt (sodium) is used.

The antioxidant can further increase the anti-dandruff effect and anti-dandruff effect of the soap composition. Antioxidants include tocopheryl acetate, butylated hydroxy anisole (BHA), ascorbyl palmitate, and etidronic acid.

The shading agent acts as a decolorizing agent to prevent the color change of the soap itself and to maintain the color of the soap inherently. Titanium dioxide, dipropylene glycol salicylate, and methyl salicylate are used as shading agents.

The detergent imparts a cleaning effect and a foaming power of the soap to improve the feeling of use of the soap and serves as a conditioner for the hair. Examples of the cleaning agent include cocamidopropyl betaine, coco-betaine, alkylbetaine, cocamide DEA, cocamide MEA, and the like, which contain an amphoteric surfactant C 8 -C 22 alkyl group. (cocamide MEA), and glyceryl cocoate.

Purified water or distilled water is used to dissolve the solid component in the additive and is added 2-4 times by weight of the solid component. In addition, preservatives, pH adjusters, perfumes, dyes, and other additives may be added to the soap composition by those skilled in the art.

Also, in the case of having a shampoo formulation, in a shampoo composition composed of a synthetic surfactant as a cleaning component, a preservative, a thickening agent and a viscosity adjusting agent, a pH adjusting agent, a perfume, a dye, a hair conditioning agent and water, May be any selected from anionic, amphoteric, and nonionic surfactants. The synthetic surfactant may be contained in an amount of 10 to 70% by weight, preferably 10 to 30% by weight, based on the total weight of the composition.

Examples of the synthetic anionic surfactant include alkyl and alkyl ether sulfates. Specific examples thereof include sodium lauryl sulfate, ammonium lauryl sulfate, triethanolamine lauryl sulfate, sodium polyoxyethylene lauryl sulfate and polyoxyethylene lauryl sulfate. have.

The synthetic amphoteric surfactants are alkyl betaines and alkylamidopropyl betaines. Specific examples thereof include cocodimethylcarboxymethylbetaine, lauryldimethylcarboxymethylbetaine, lauryldimethyl alpha-carboxyethylbetaine, cetyldimethylcarboxymethyl Betaine, cocamidopropyl betaine, and the like.

Examples of the synthetic nonionic surfactant include alkanolamide and amine oxide. Specific examples thereof include lauryldiethylamine oxide, palm oil alkyldimethylamine oxide, lauric acid diethanolamide, palm oil fatty acid diethanolamide, palm oil fatty acid monoethanolamide .

The above-mentioned surfactants can be selectively used alone or in combination in the composition of the present invention.

The optional ingredients used in the compositions of the present invention are well known to those skilled in the art as conventional optional ingredients to maintain basic properties and quality. Such optional ingredients include preservatives, thickeners, viscosity modifiers, pH adjusting agents, perfumes, dyes, hair conditioning agents, and the like.

The composition according to the present invention can be used according to a conventional method of use, and may be used in a number of times depending on a user's skin condition, inflammation condition, scalp condition or taste.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples. However, the present invention is not limited to these examples. In the following examples, an ice plant juice was used, but an ice plant powder, an ice plant extract, It is self-evident that the same effect is exhibited even when a mixture is used.

[ Example 1] Ice Plant Juice Produce

The ground portion except the roots of the ice plant directly grown on the farm of the present inventor was sampled and put into a green juice and juice was used for the following tests and preparation of the composition.

Test Example 1: immunological activity test

The effect of the cell-mediated immunity on the juice prepared in Example 1 was measured by the NF-kB inhibition test.

The NF-kB promoter has been reported to play an important role in the production of immune-related cytokines. Since the NF-kB factor is known to be an important factor in the expression of IL-8 and tumor necrosis factor-alpha (TNF-α), which are typical cytokines related to inflammation, Inhibiting the activity of NF-kB luciferase is highly likely to inhibit the activity of inflammation-related cytokines.

TNF-α is a cytotoxic monocaine produced by stimulated macrophages and plays an important role in the recovery of inflammation and wound. Therefore, we examined whether a substance stimulates macrophages to induce TNF-α biosynthesis, or test whether TNF-α affects the efficacy of TNF-α by culturing TNF-α and a sample to be tested in a TNF-α sensitive cell line The ability to regulate immunity can be investigated.

Test Methods

1 × ¹⁰ 6 cells were placed in 6 wells of human monocytic THP-1 cells, and NF-kB luciferase plasmid DNA (NF-kB luciferase) was prepared using a superfect transfection reagent reporter plasmid DNA) was transfected. Twenty-four hours after transfection, lipopolysaccharide (LPS) (100 ng / ml) was treated to activate THP-1 cells and each extract was treated at different concentrations. After 24 hours, the cells were collected and luciferase activity was measured using a luminometer.

The results of NF-kB promoter inhibition according to the concentration of each juice were shown in Table 1.

density NF-kB promoter inhibition result LPS 26,597 0.5 wt% 20,594 1.0 wt% 15,417 2.0 wt% 12,254 4.0 wt% 12,248 8.0 wt% 12,211

As shown in Table 1, it was confirmed that the ice plant juice according to the present invention exhibited excellent effects.

Test Example 2: Antibacterial effect test

The antimicrobial effect of the juice prepared in Example 1 was measured using a minimum inhibitory concentration (MIC) test.

MIC test This is a method to measure the growth of the test organism after the raw material having the antimicrobial effect is added in a stepwise concentration in the liquid medium, the inoculum of the various test organisms is inoculated thereto, the shake culture is carried out at 25 ° C for 24 hours.

Test Methods

Each microorganism to be tested is cultured in 10 ml of yeast peptone dextrose broth at 25 ° C for 48 hours, and then the microorganisms are inoculated so that each cultured microorganism becomes 10 ⁴ cells in 3 ml of medium. Add the juice to each medium by concentration. After culturing the prepared test tube at 25 ° C for 48 hours, the degree of growth of the microorganism is observed.

For each concentration of juice, acne bacterium Propionibacterium acnes ( Propionibacterium acnes) acnes KCTC3314), Candida albicans (ATCC 13018), vaginitis bacteria, Pityrosporum Ovalae (ATCC 12078)) showed the antimicrobial activity as shown in Table 2.

density Propionate bacterium acnes Candida albicans Pete Roth Formo Valle 0.5 wt% - - - 1.0 wt% - - - 2.0 wt% - - - 4.0 wt% - - - 8.0 wt% - - - +: Microbial growth appears
-: No microbial growth due to antimicrobial activity

As shown in Table 2, it was confirmed that the ice plant juice according to the present invention showed excellent antibacterial and antifungal effects even at 0.5 wt%.

Test Example 3: Toxicity test

The cytotoxic effect of the juice prepared in Example 1 was measured using cell culture.

Way

The medium used for the cytotoxicity test (Mosmann, T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays, J. Immunol. Meth. 1983, 65, 55-63) The human fibroblast cell line (ATCC, CRL-2076) is inoculated into DMEM at a concentration of 1 × 10 ⁵ cells / ml in a 24-well plate. After 24 hours of sowing, the medium is changed, and the juice is added by concentration. The cells are cultured in a 5% CO ² incubator at 37 ° C for 3 days. After MTT solution was added at a rate of 1 ml / well, the cells were cultured in a CO ² incubator at 37 ° C for about 4 hours. After removing the medium, 200 μl of DMSO was added thereto and the absorbance was measured at 550 nm.

result

From 0.5 wt% to 8 wt% tested, no cell growth inhibition was observed in the juice of Example 1, and it was found that it was safe to use in the detergent composition in the evaluation of cytotoxicity.

[ Formulation Example 1] Preparation of composition 1

Soaps were prepared with the composition shown in Table 3 below, and the manufacturing process was in accordance with a method widely known to those skilled in the art.

ingredient Formulation Example 1 (% by weight) Distilled water 15.60 Sodium palm oil 51.65 glycerin 16.00 Sorbitol 8.20 Sodium chloride 0.50 Ice plant juice 7.00 Sodium hyaluronate 1.00 Tocopherol 0.05 total 100

[ Formulation Example 2] Preparation of composition 2

Shampoos were prepared in the composition shown in Table 4 below, and the manufacturing process was in accordance with a method widely known to those skilled in the art.

ingredient Formulation Example 2 (% by weight) Ammonium lauryl sulfate 10 Ammonium polyoxyethylene uryl sulfate 5 Cocoamidopropyl betaine 2 Ethylene glycol distearate 1.5 Cottoyl monoethanolamide 0.8 Ice plant juice 5.0 Polyquaternium-10 0.2 Blue No. 1 0.0002 Yellow No. 4 0.0001 Methyl paraben 0.1 Spices 0.8 Citric acid 0.1 Dimethicone 1.0 Distilled water to 100

[ Test Example 4] Dandruff reduction test

Forty - eight males aged 19 to 35 years with a relatively large number of dandruff were selected and divided into two groups. Dandruff reduction rates were measured after each group for each month using the following method.

The dandruff accumulated for 2 days before the start of the test was collected, and the weight of collected dandruff was compared with the weight of the dandruce accumulated for 2 days after completion of the test. The accumulated dandruff was collected directly from the scalp using a vacuum suction device, and the dandruff reduction rate was calculated according to the following equation (1). The results are shown in Table 5 below.

&Quot; (1) "

Dandruff reduction rate (%) = pre-test dandruff weight (mg) -1 month later dandruff weight (mg) / pre-test dandruff weight (mg) x 100

Formulation Example 1 Formulation Example 2 90.1 89.9 90.1 77.8 90.5 90.1 89.8 85.2 99.2 89.5 95.2 90.2 91.3 89.0 90.5 81.9 89.0 90.2 91.9 89.0 93.2 90.2 92.0 88.3

As can be seen from the above Table 5, the excellent dandruff-preventing effect was confirmed, and in particular, the formulation according to the present invention showed a more excellent anti-dandruff effect in the soap composition.

[ Test Example 5] Scalp itching prevention effect test

A total of 36 men and women aged 25 to 45 years with severe scalp itching were selected and divided into two groups of 12 persons. Each formulation was used once every 3 days for 2 weeks. The results are shown in Table 6 below.

[Evaluation standard]

Very good - 5 points

Excellent - 4 points

Usually - 3 points

Poor - 2 points

Very bad - 1 point

division Formulation Example 1 Formulation Example 2 Scalp itching removal effect 4.6 4.3

As can be seen from Table 6, the formulation containing the ice plant juice according to the present invention had an excellent effect of preventing itching. In particular, the formulation 1 according to the present invention, which is a soap, It was found that it shows an effect of preventing itching.

[ Test Example 6] Anti-hair loss test

In order to confirm the effect of preventing hair loss, 10 of 40 male subjects with alopecia were given Formulation Examples 1 and 2, respectively, and the hair was applied once a day. The duration of use was 3 months, . The evaluation criteria were as follows: 5 points when the hair loss reduction effect was significant, 4 points when moderate hair loss, 3 points when the hair loss was severe, 2 points when the hair loss was unchanged, and 1 point when the hair loss symptom worsened And a comprehensive judgment was made as an average value.

division Formulation Example 1 Formulation Example 2 Anti-hair loss effect 4.6 4.5

As can be seen from the above Table 7, the formulation containing the aasplant juice according to the present invention was excellent in the effect of preventing hair loss. In particular, the formulation 1 according to the present invention, which is a soap, And it was found that it showed the effect of preventing it.

[ Test Example 7] Itching reduction test

Thirty women aged 25 to 45 years who were very sensitive to itching were selected and divided into two groups of 15 each. Each formulation was used once a day for two weeks each time, and the itching effect was evaluated through the following evaluation criteria. Are shown in Table 8 below.

[Evaluation standard]

Very good - 5 points

Excellent - 4 points

Usually - 3 points

Poor - 2 points

Very bad - 1 point

division Formulation Example 1 Formulation Example 2 Itching effect 4.5 4.1

As can be seen from Table 8, it was found that the itching-preventing effect of the formulation containing the ice plant of the present invention was excellent.

[ Example 2 and Formulation Example 3] Preparation of composition 3

The present embodiment and the formulation example are the same as the above-described Example 1 and Formulation Example 1, except that only the flower bud of the ice plant is used in producing the ice plant juice.

[ Test Example 8] Itching reduction test

The same tests as in Test Example 5 and Test Example 7 were conducted in Formulation Example 3, which was prepared using an ice plant flower bud juice, and the results are shown in Table 9 below.

division Scalp itching removal effect Itching effect Formulation Example 3 4.9 4.9

As shown in Table 9, it was found that the effect was excellent in relieving the itching of scalp and female vagina.

[ Formulation Example 4] Preparation of composition 4

As shown in the following Table 10, soaps were prepared with a composition further containing a hot water extract of Acacia japonica, and the manufacturing process was in accordance with a method widely known to those having ordinary skill in the art to which the present invention belongs.

ingredient Formulation Example 4 (% by weight) Distilled water 15.60 Sodium palm oil 44.65 glycerin 16.00 Sorbitol 8.20 Sodium chloride 0.50 Ice plant juice 7.00 Acid hydrothermal extract 7.00 Sodium hyaluronate 1.00 Tocopherol 0.05 total 100

[ Test Example 9] Hair loss prevention test

The anti-hair loss effect of Formulation Example 4 was measured. The results are shown in Table 11, and the measurement method was the same as in Test Example 6.

division Formulation Example 3 Hair loss prevention effect 4.9

Table 11 shows better effects than the case of containing only ice plant juice.

According to the above test results, the detergent composition containing the ice plant according to the present invention has excellent cytotoxicity and safety, and it can suppress sebum control effect and anti-inflammatory effect, that is, And is excellent in the antibacterial effect against dandruff, and is excellent in antidandruce treatment and dandruff prevention effect, and has a hair loss prevention effect.

Claims

And 7% by weight of an ice plant juice and 7% by weight of a hydrothermal extract of Sanssheniaceae, based on the total weight of the composition.

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