JP2013155139A - Composition and cosmetic - Google Patents

Composition and cosmetic Download PDF

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JP2013155139A
JP2013155139A JP2012017338A JP2012017338A JP2013155139A JP 2013155139 A JP2013155139 A JP 2013155139A JP 2012017338 A JP2012017338 A JP 2012017338A JP 2012017338 A JP2012017338 A JP 2012017338A JP 2013155139 A JP2013155139 A JP 2013155139A
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JP6208407B2 (en
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Seiichi Kitamura
整一 北村
Masahito Tsubata
仁人 鍔田
Kazuya Yamaguchi
和也 山口
Kinya Takagaki
欣也 高垣
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Toyo Shinyaku Co Ltd
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Abstract

PROBLEM TO BE SOLVED: To provide a composition for inhibiting collagenase activity having an excellent collagenase activity inhibiting action, a composition for improving wrinkles or sagging, a composition for improving the tension of skin, a composition for improving the texture of skin, a composition for maintaining the elasticity of skin, a composition for beauty and an anti-aging composition, and cosmetics including the compositions of them.SOLUTION: A composition for inhibiting collagenase activity, a composition for improving wrinkles or sagging, a composition for improving the tension of skin, a composition for improving the texture of skin, a composition for maintaining the elasticity of skin, a composition for beauty and an anti-aging composition include an extract extracted from a plant body of the Aizoaceae. Cosmetics include the compositions of them. Preferably, the plant body of the Aizoaceae is the plant body included in an ice plant.

Description

本発明は、ハマミズナ科の植物体から得られた抽出物を含有する新規なコラゲナーゼ活性阻害用組成物、シワ又はタルミの改善用組成物、皮膚のハリ改善用組成物、皮膚のキメ改善用組成物、皮膚の弾力性維持用組成物、美容用組成物、及び抗老化用組成物、並びに、これらの組成物を含有する化粧料に関する。   The present invention relates to a novel collagenase activity-inhibiting composition containing an extract obtained from a plant of the genus Camellia, a composition for improving wrinkles or tarmi, a composition for improving skin firmness, and a composition for improving skin texture The present invention relates to a product, a composition for maintaining skin elasticity, a cosmetic composition, an anti-aging composition, and a cosmetic containing these compositions.

皮膚の表皮及び真皮は、表皮細胞、線維芽細胞及びこれらの細胞の外にあって皮膚構造を支持するエラスチン、コラーゲン等の細胞外マトリックスにより構成されている。若い皮膚においては、これら皮膚組織の相互作用が恒常性を保つことにより水分保持、柔軟性、弾力性等が確保され、肌は外見的にも張りや艶があってみずみずしい状態に維持される。   The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin and collagen that are outside these cells and support the skin structure. In young skin, moisture retention, flexibility, elasticity and the like are ensured by maintaining the constancy of the interaction between these skin tissues, and the skin is maintained in a fresh and fresh state.

ところが、紫外線の照射、空気の著しい乾燥、過度の皮膚洗浄等、ある種の外的因子の影響があったり、加齢が進んだりすると、細胞外マトリックスの主要構成成分であるエラスチンは分解又は変質を起こし、また、コラーゲンの産生量が減少するとともに架橋による弾力低下を起こす。その結果、皮膚は保湿機能や弾力性が低下し、角質は異常剥離を始めるため、肌は張りや艶を失い、荒れ、シワ等の老化症状を呈するようになる。   However, elastin, which is a major component of the extracellular matrix, degrades or deteriorates when affected by certain external factors such as UV irradiation, significant drying of the air, excessive skin cleansing, and so on. In addition, as the amount of collagen produced decreases, the elasticity decreases due to crosslinking. As a result, the skin's moisturizing function and elasticity are lowered, and the horny layer begins to exfoliate abnormally, so that the skin loses its tension and gloss, and exhibits aging symptoms such as roughness and wrinkles.

このように、皮膚の老化に伴う変化、即ち、シワ、くすみ、きめの消失、弾力性の低下等には、コラーゲン、エラスチン等の真皮細胞外マトリックス成分の減少、変性が関与している。   As described above, changes associated with skin aging, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, and the like involve reduction and degeneration of dermal extracellular matrix components such as collagen and elastin.

近年、この変化を誘導する因子として、特にマトリックス系プロテアーゼの関与が指摘されている。マトリックス系プロテアーゼの中でも、コラゲナーゼ、即ちMMP−1(マトリックスメタロプロテアーゼ)は、皮膚の真皮細胞外マトリックスの主な構成成分であるタイプI、IIIコラーゲンを分解する酵素として知られるが、その発現は紫外線の照射により大きく増加し、紫外線によるコラーゲンの減少・変性の一因となり、皮膚のシワの形成等の大きな要因となることが考えられる。したがって、コラゲナーゼ活性の阻害は、シワやタルミの形成阻止、肌荒れ改善等の美容、皮膚の老化防止及び改善に重要である。   In recent years, involvement of matrix proteases has been pointed out as a factor inducing this change. Among the matrix proteases, collagenase, that is, MMP-1 (matrix metalloprotease) is known as an enzyme that degrades type I and III collagens, which are main components of the dermal extracellular matrix of the skin. It is considered that it is greatly increased by irradiation of UV rays, contributes to decrease / denaturation of collagen by ultraviolet rays, and is a major factor such as the formation of wrinkles on the skin. Therefore, inhibition of collagenase activity is important for the prevention of wrinkles and tarmi formation, beauty such as improvement of rough skin, prevention of skin aging and improvement.

そのため、コラーゲンの分解を抑制し、シワ又はタルミの形成を防止し、皮膚の老化を防止できるコラゲナーゼ活性阻害用組成物となり得る種々の物質が研究されている。   Therefore, various substances that can be used as a collagenase activity-inhibiting composition that can suppress collagen degradation, prevent formation of wrinkles or tarmi, and prevent skin aging have been studied.

現在までに、コラゲナーゼ活性阻害作用を有する植物の代表的なものとして、コーヒー酸又はその塩(特許文献1参照)、グアバの葉、西河柳、菱実(特許文献2参照)、ハマメリス抽出物(特許文献3参照)、コケモモ、シラカバ、ハマスゲ、ボダイジュ、緑茶(特許文献4参照)、シモツケソウ、ヤドリギ、ホホバ葉、蓮葉、レッドクローバー(特許文献5参照)、松樹皮抽出物(特許文献6参照)、発芽ケール(特許文献7)、カムカム種子の抽出物(特許文献8参照)、アセロラ種子の抽出物(特許文献9参照)などが知られている。このように、コラゲナーゼ活性阻害作用を有するものとして従来より植物由来の組成物が、その安全性や刺激の穏やかさを期待して種々開発されているが、今までにコラゲナーゼ活性阻害作用が見出されていなかった新しい植物を用いたコラゲナーゼ活性阻害用組成物、シワ又はタルミの改善用組成物、皮膚のハリ改善用組成物、皮膚のキメ改善用組成物、皮膚の弾力性維持用組成物、美容用組成物、及び抗老化用組成物の更なる開発が求められている。   To date, representative examples of plants having an inhibitory activity on collagenase activity include caffeic acid or a salt thereof (see Patent Document 1), guava leaf, Nishikawayanagi, Rishimi (see Patent Document 2), Hamamelis extract ( Patent Document 3), Cowberry, Birch, Hamasge, Bodaiju, Green Tea (refer to Patent Document 4), Shimotsuke, Mistletoe, Jojoba Leaf, Lotus Leaf, Red Clover (refer to Patent Document 5), Pine Bark Extract (refer to Patent Document 6) Germination kale (patent document 7), camcam seed extract (see patent document 8), acerola seed extract (see patent document 9) and the like are known. As described above, various plant-derived compositions have been developed as having an activity of inhibiting collagenase activity, with the expectation of safety and mildness of stimulation. A composition for inhibiting collagenase activity using a new plant that has not been prepared, a composition for improving wrinkles or tarmi, a composition for improving skin firmness, a composition for improving skin texture, a composition for maintaining skin elasticity, There is a need for further development of cosmetic compositions and anti-aging compositions.

特開平5−117145号公報JP-A-5-117145 特開平7−291873号公報JP-A-7-291873 特開平8−283133号公報JP-A-8-283133 特開2003−12531号公報JP 2003-12531 A 特開2003−48846号公報JP 2003-48846 A 特開2003−238426号公報JP 2003-238426 A 特開2010−037320号公報JP 2010-037320 A 特許第3635081号公報Japanese Patent No. 3635081 特開2007−314552号公報JP 2007-314552 A

本発明は、上記事情に鑑みてなされたものであり、以下の目的を達成することを課題とする。即ち、本発明は、優れたコラゲナーゼ活性阻害作用を有するコラゲナーゼ活性阻害用組成物、シワ又はタルミの改善用組成物、皮膚のハリ改善用組成物、皮膚のキメ改善用組成物、皮膚の弾力性維持用組成物、美容用組成物、及び抗老化用組成物、並びに、これらの組成物を含有する化粧料を提供することを目的とする。   This invention is made | formed in view of the said situation, and makes it a subject to achieve the following objectives. That is, the present invention relates to a collagenase activity inhibiting composition having an excellent collagenase activity inhibiting action, a composition for improving wrinkles or tarmi, a composition for improving skin firmness, a composition for improving skin texture, and skin elasticity. It is an object of the present invention to provide a maintenance composition, a cosmetic composition, an anti-aging composition, and a cosmetic containing these compositions.

本発明者らは、前記目的を達成するため鋭意検討を行った結果、ハマミズナ科の植物体の抽出物が優れたコラゲナーゼ活性阻害作用を有することを見出し、本発明の完成に至った。   As a result of intensive studies to achieve the above-mentioned object, the present inventors have found that an extract of a plant of the family Vaccinaceae has an excellent collagenase activity inhibitory action, leading to the completion of the present invention.

本発明は、本発明者らによる前記知見に基づくものであり、前記課題を解決するための手段としては、以下の通りである。即ち、
<1> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とするコラゲナーゼ活性阻害用組成物である。
<2> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とするシワ又はタルミの改善用組成物である。
<3> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とする皮膚のハリ改善用組成物である。
<4> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とする皮膚のキメ改善用組成物である。
<5> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とする皮膚の弾力性維持用組成物である。
<6> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とする美容用組成物である。
<7> ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とする抗老化用組成物である。
<8> 抽出物が、ハマミズナ科の植物体を細分又は圧搾したものから抽出されたものである前記<1>から<7>のいずれかに記載の組成物である。
<9> ハマミズナ科の植物体が、メセンブリアンテマ属の植物体である前記<1>から<8>のいずれかに記載の組成物である。
<10> ハマミズナ科の植物体が、アイスプラントに含まれる植物体である前記<1>から<9>のいずれかに記載の組成物である。
<11> ハマミズナ科の植物体が、前記植物体の地上部である前記<1>から<10>のいずれかに記載の組成物である。
<12> ハマミズナ科の植物体が、養液栽培されたものである前記<1>から<11>のいずれかに記載の組成物である。
<13> 前記<1>から<12>のいずれかに記載の組成物を含有することを特徴とする化粧料である。
The present invention is based on the above findings by the present inventors, and means for solving the above problems are as follows. That is,
<1> A collagenase activity-inhibiting composition characterized by containing an extract extracted from a plant body of the family Ciraceae.
<2> A composition for improving wrinkles or talmi, which contains an extract extracted from a plant body of the family Ciraceae.
<3> A composition for improving the elasticity of skin, comprising an extract extracted from a plant of the family Occidentalis.
<4> A composition for improving skin texture, which comprises an extract extracted from a plant body of the family Ciraceae.
<5> A composition for maintaining the elasticity of the skin, comprising an extract extracted from a plant body of the family Ciraceae.
<6> A cosmetic composition characterized by containing an extract extracted from a plant body of the family Ciraceae.
<7> An anti-aging composition characterized by containing an extract extracted from a plant body of the family Ciraceae.
<8> The composition according to any one of <1> to <7>, wherein the extract is extracted from a submerged or squeezed plant of the family Alaska.
<9> The composition according to any one of the items <1> to <8>, wherein the plant of the family Hydromyceae is a plant of the genus Mesembriantema.
<10> The composition according to any one of <1> to <9>, wherein the plant body of the family Ciraceae is a plant body contained in an ice plant.
<11> The composition according to any one of <1> to <10>, wherein a plant body of the family Ciraceae is an above-ground part of the plant body.
<12> The composition according to any one of <1> to <11>, wherein the plant body of the family Hydonicaceae is one that has been hydroponically cultivated.
<13> A cosmetic comprising the composition according to any one of <1> to <12>.

本発明によれば、従来における前記諸問題を解決し、前記目的を達成することができ、優れたコラゲナーゼ活性阻害作用を有するコラゲナーゼ活性阻害用組成物、シワ又はタルミの改善用組成物、皮膚のハリ改善用組成物、皮膚のキメ改善用組成物、皮膚の弾力性維持用組成物、美容用組成物、及び抗老化用組成物、並びに、これらの組成物を含有する化粧料を提供することができる。   ADVANTAGE OF THE INVENTION According to this invention, the said problems in the past can be solved, the said objective can be achieved, the composition for collagenase activity inhibition which has the outstanding collagenase activity inhibitory effect, the composition for wrinkle or talmi improvement, skin To provide a firmness improving composition, a skin texture improving composition, a skin elasticity maintaining composition, a cosmetic composition, an anti-aging composition, and a cosmetic containing these compositions Can do.

図1は、実施例1におけるアイスプラント(バラフ)抽出物のコラゲナーゼ阻害率を示したグラフである。1 is a graph showing the collagenase inhibition rate of an ice plant (barafu) extract in Example 1. FIG.

(コラゲナーゼ活性阻害用組成物、シワ又はタルミの改善用組成物、皮膚のハリ改善用組成物、皮膚のキメ改善用組成物、皮膚の弾力性維持用組成物、美容用組成物、及び抗老化用組成物)
本発明のコラゲナーゼ活性阻害用組成物、シワ又はタルミの改善用組成物、皮膚のハリ改善用組成物、皮膚のキメ改善用組成物、皮膚の弾力性維持用組成物、美容用組成物、及び抗老化用組成物(以下では、これらを単に「組成物」と呼ぶこともある)は、少なくとも、ハマミズナ科の植物体から抽出した抽出物を含有してなり、更に必要に応じて、その他の成分を含有してなる。前記組成物によれば、後述する実施例で示すように、優れたコラゲナーゼ活性阻害作用を有するため、コラゲナーゼ活性阻害用、シワ又はタルミの改善用、皮膚のハリ改善用、皮膚のキメ改善用、皮膚の弾力性維持用、美容用、及び抗老化用のいずれの用途にも有効である。
(Composition for inhibiting collagenase activity, composition for improving wrinkles or tarmi, composition for improving skin firmness, composition for improving skin texture, composition for maintaining skin elasticity, cosmetic composition, and anti-aging Composition)
Composition for inhibiting collagenase activity, composition for improving wrinkles or tarmi, composition for improving skin firmness, composition for improving skin texture, composition for maintaining skin elasticity, cosmetic composition, and The composition for anti-aging (hereinafter, sometimes simply referred to as “composition”) contains at least an extract extracted from a plant of the family Apoaceae, and, if necessary, other Contains ingredients. According to the composition, as shown in the examples described later, since it has an excellent collagenase activity inhibitory action, for collagenase activity inhibition, wrinkle or tarmi improvement, skin firmness improvement, skin texture improvement, It is effective for any use for maintaining skin elasticity, cosmetics, and anti-aging.

ここで、ハマミズナ科(Aizoaceae)とは、双子葉植物の科で、約126属1,100種を含む。なお、ハマミズナ科は、呼び名に混乱があり、ツルナ科と呼ばれることも多いが、ツルナ属(Tetragonia)とTribulocarpus属を狭義のツルナ科(Tetragoniaceae)として分ける場合には、他の属の植物にはハマミズナ科の名が用いられる(ただし、現在は、ツルナもハマミズナ科に含めるのが普通である)。ハマミズナ科は、別名としてマツバギク科、メセン科などの通称も使われる。学名もTetragoniaceaeFicoideaeなどの旧称が使われることがある。また、かつてはザクロソウ科(Molluginaceae)と同じ分類とされていたため、古い図鑑には「ザクロソウ科 Aizoaceae」などの表記もみられる。本明細書では、これらの用語の混乱を避けるために、ハマミズナ科(Aizoaceae)とは、ツルナ科、マツバギク科、メセン科を含み、かつてはザクロソウ科に分類されていた属乃至種を含む概念であるものとする。 Here, the family Azoaceae is a family of dicotyledonous plants and includes about 1,100 species of about 126 genera. It should be noted, aizoaceae is, there is confusion in the nickname, but it is also often referred to as Crna family, if the divide as Crna genus (Tetragonia) and Tribulocarpus genus narrow sense of Crna Department (Tetragoniaceae) is, in the other plant of the genus The name of the clamaceae is used (but now it is common to include tsuruna in the clamaceae). As for the family Occidentalis, common names such as pineapple family and mesenaceae are used as aliases. Scientific names are sometimes used with old names such as Tetragoniasae and Ficoideae . Moreover, since it was once classified into the same class as Pomegranateaceae ( Molulinaceae ), notations such as “Azaaceaeaceae Azoaceae ” are also seen in the old pictorial book. In this specification, to avoid confusion of terminology, an aizoaceae (Aizoaceae), Crna family, Matsubagiku family comprises Looking family, once a concept including a genus or species that have been classified into molluginaceae It shall be.

また、メセンブリアンテマ属(Mesembryanthemum)とは、ハマミズナ科に含まれる多肉植物の属であり、約50種を含む。このメセンブリアンテマ属には、例えば、Mesembryanthemum aitonisMesembryanthemum barklyiMesembryanthemum cryptanthumMesembryanthemum crystallinumMesembryanthemum excavatumMesembryanthemum fastigiatumMesembryanthemum gariusanumMesembryanthemum guerichianumMesembryanthemum hypertrophicumMesembryanthemum inachabenseMesembryanthemum lomgistylumMesembryanthemum nodiflorumMesembryanthemum pellitumMesembryanthemum stenandrumMesembryanthemum subtruncatumMesembryanthemum teretifoliumMesembryanthemum teretiusculumMesembryanthemum ventricosumMesembryanthemum violenseなどの種が含まれる。 The genus Mesembryanthema is a genus of succulents included in the family Auricularia, and includes about 50 species. The Mesenburiantema genus, for example, Mesembryanthemum aitonis, Mesembryanthemum barklyi, Mesembryanthemum cryptanthum, Mesembryanthemum crystallinum, Mesembryanthemum excavatum, Mesembryanthemum fastigiatum, Mesembryanthemum gariusanum, Mesembryanthemum guerichianum, Mesembryanthemum hypertrophicum, Mesembryanthemum inachabense, Mesembryanthemum lomgistylum, M esembryanthemum nodiflorum, Mesembryanthemum pellitum, Mesembryanthemum stenandrum , Mesembryanthemum subtruncatum, Mesembryanthemum teretifolium, Mesembryanthemum teretiusculum, Mesembryanthemum ventricosum, includes species such as Mesembryanthemum violense.

前記アイスプラントは、ハマミズナ科メセンブリアンテマ属に含まれる植物の種名である。表皮に塩を隔離するための細胞があるため葉の表面が凍ったように見えることが、名前の由来となっている。アイスプラントは、南アフリカ原産、ヨーロッパ、西アジア等に自生の種である。アイスプラントは、乾燥に耐えるとともに、耐塩性が高い塩生植物の一つであり、海水と同程度の塩化ナトリウム水溶液中でも水耕栽培が可能である。更に、アイスプラントは、生活環が半年程度と比較的短く、栽培も容易なため、植物の耐塩性研究におけるモデル生物と考えられ、学術的な注目も集まっている。しかしながら、アイスプラントは、水耕栽培等の養液栽培ではなく土耕栽培を行った場合はエグ味成分が蓄積しやすく、また地中にカドミウムなどの有害な重金属が含まれている場合、一般作物以上に吸収、蓄積する場合があるため、養液栽培されたものが好ましい。   The ice plant is a species name of a plant included in the genus Mesembrianta. The name is derived from the fact that the surface of the leaves look frozen because there are cells in the epidermis to isolate salt. Ice plants are native to South Africa, native to Europe and West Asia. The ice plant is one of halophytic plants that resists drying and has high salt tolerance, and can be hydroponically cultivated even in an aqueous sodium chloride solution similar to seawater. Furthermore, the ice plant is considered to be a model organism in research on salt tolerance of plants because its life cycle is relatively short, about half a year, and easy to grow, and has attracted academic attention. However, ice plants tend to accumulate savory components when soil cultivation is performed instead of hydroponics such as hydroponics, and when the ground contains harmful heavy metals such as cadmium, Since it may absorb and accumulate more than crops, it is preferable to use hydroponics.

前記養液栽培に用いられる養液及び栽培方法としては、例えば、特開2009−254246号公報に記載されたものを用いることができる。   As the nutrient solution and the cultivation method used for the nutrient solution cultivation, for example, those described in JP 2009-254246 A can be used.

前記アイスプラントは、非常に特徴的なことに、乾燥や塩ストレスを与えないと、一般的な光合成経路であるC3光合成を行い、乾燥又は塩ストレスを与えるとCAM型光合成へ移行することができる。また、表皮には塩嚢細胞(ブラッダー細胞、英語:Bladder Cell)と呼ばれる体内に侵入した塩類を隔離するための細胞が発達する。この塩嚢細胞の大きさは2mm前後に達し、透明でキラキラと輝くため、種名のクリスタリナムの由来となっている。   It is very characteristic that the ice plant performs C3 photosynthesis, which is a general photosynthesis route, when it is not subjected to drying or salt stress, and can shift to CAM type photosynthesis when subjected to drying or salt stress. . In the epidermis, cells that sequester salts that have entered the body, called capsular cells (Bladder cells, English: Blader Cell), develop. The size of this sac cell is about 2 mm, and it is transparent and shining, so it is the origin of the name Crystallinam.

アイスプラントは、日本でも佐賀大学農学部が栽培化し、塩味のする新野菜として紹介されたことをきっかけに、近年、全国各地でも栽培されはじめている。国立ファームはソルトリーフ(登録商標)、佐賀大学発ベンチャーの株式会社農研堂はバラフ(登録商標)とクリスタルリーフ(登録商標)、アグリ社はプッチーナ(登録商標)、滋賀県長浜市の日本アドバンストアグリはツブリナ(登録商標)という商標を用いている。また、静岡県ではソルティーナやシオーナの名で販売されている。本明細書では、これらの用語の混乱を避けるために、アイスプラント(学名:Mesembryanthemum crystallinum)には、バラフ、クリスタルリーフ、プッチーナ、ツブリナ、ソルティーナ、シオーナなどが含まれるものとする。 In recent years, ice plants have been cultivated in Japan and have been cultivated by the Faculty of Agriculture of Saga University and introduced as a new salty vegetable. National Farm is Salt Reef (registered trademark), Saga University's Venture Co., Ltd., Nara Kendo Co., Ltd. is Baraf (registered trademark) and Crystal Leaf (registered trademark), Agri is Puccina (registered trademark), Japan Advanced Aguri in Nagahama City, Shiga Prefecture Uses the trademark Tubulina (registered trademark). In Shizuoka Prefecture, it is sold under the names of Soltina and Siona. In this specification, in order to avoid confusion with these terms, an ice plant (scientific name: Mesebryanthem crystallinum ) includes baraph, crystal leaf, puccina, tubulina, saltina, siona, and the like.

ここで、「バラフ」とは、佐賀大学農学部で研究開発されている、南アフリカ原産の植物「アイスプラント」を野菜化した佐賀大学ブランド商品であり、市販されている。葉や茎の表面に宝石のようにキラキラと光る水滴のような細胞がついているのが、大きな特徴である。その外観から、アフリカのスワヒリ語で「水晶」や「氷」を意味する「バラフ」と名付けられている。バラフは、佐賀大学発ベンチャー企業(株)農研堂と契約農家で栽培を行っており、佐賀大学農学部で開発された技術によって品質安定を図り、安全性・安心に配慮して栽培されている。   Here, “barafu” is a Saga University brand product that is researched and developed by the Faculty of Agriculture of Saga University and made into a vegetable from the plant “ice plant” native to South Africa, and is commercially available. The main feature is that the surface of leaves and stems have sparkling water droplets like jewels. Because of its appearance, it is named “Baraf” meaning “crystal” or “ice” in Swahili. Barraf is cultivated in a contract farm with Saga University's venture company, Kenkendo Co., Ltd., and is cultivated in consideration of safety and security by stabilizing the quality with the technology developed by the Faculty of Agriculture of Saga University.

なお、プチプチとした食感で、塩味の新野菜として注目を集めているアイスプラントの栽培について、商品化した佐賀大学農学部の研究グループが、土での栽培に注意を呼び掛けている。主要な県産品は養液栽培だが、土耕栽培の場合、土に重金属が含まれると吸収、蓄積する可能性があるためである。したがって、ハマミズナ科の植物体を用いる場合には、養液栽培されたものを用いることが好ましい。   A research group from the Faculty of Agriculture of Saga University, which has been attracting attention as a new salty vegetable with a soft texture, calls for attention on soil cultivation. The main prefectural product is hydroponics, but in the case of soil cultivation, if heavy metals are contained in the soil, it can be absorbed and accumulated. Therefore, in the case of using a plant of the genus Chironomidae, it is preferable to use one that has been hydroponically cultivated.

佐賀大のグループは、もともと干拓地の塩害改善作物としてアイスプラントの研究をしていた。佐賀大のグループは、アイスプラントの食用も開発し「バラフ」の名称で商標登録をした。佐賀大のグループは、栽培方法としては、「地中にカドミウムなどの有害な重金属が含まれている場合、一般作物以上に吸収、蓄積する特性があった」(野瀬昭博教授)として養液栽培を選択している。また、栽培に使用する培地についても分析を行い、栽培に使用する水は飲料適水に限定している。同教授は、土耕栽培をする場合には、「重金属の有無など、精密に土壌分析したうえで栽培すべき」と注意を喚起している。   The group at Saga University originally studied ice plants as a crop for improving salt damage in the reclaimed land. A group at Saga University developed an edible ice plant and registered a trademark under the name “Baraf”. The group at Saga University has developed a hydroponic cultivation method as follows: “When the ground contains toxic heavy metals such as cadmium, it absorbs and accumulates more than general crops” (Prof. Akihiro Nose) Is selected. Moreover, it analyzes also about the culture medium used for cultivation and the water used for cultivation is limited to drink suitable water. The professor has cautioned that when cultivating soil, it should be cultivated after precise soil analysis, such as the presence or absence of heavy metals.

これらのハマミズナ科の植物体(例えば、アイスプラント)を用いる場合には、前期植物体の地上部を用いることが好ましい。ハマミズナ科の植物体(例えば、アイスプラント)のうち、地上部が特に食用として適しているため、本発明の組成物に用いる抽出物も食履歴などに基づく安全性及び健康によい成分を多く含むことが期待されることなどの観点から地上部を用いることが好ましいためである。ここで、本明細書において、植物体の「地上部」とは、地表よりも上部に位置する部位(葉、茎など)を含む概念である。   When these plant species (for example, ice plant) are used, it is preferable to use the above-ground part of the plant body. Among the plants (for example, ice plants) of the family Auricularia, the above-ground part is particularly suitable for food, so the extract used in the composition of the present invention also contains many components that are good for safety and health based on food history and the like. This is because it is preferable to use the above-ground part from the standpoint of what is expected. Here, in the present specification, the “ground part” of the plant body is a concept including a part (a leaf, a stem, etc.) located above the ground surface.

<抽出物>
前記抽出物は、前記ハマミズナ科の植物体から抽出されてなる。抽出方法としては、特に制限はなく、目的に応じて適宜選択することができるが、ハマミズナ科の植物体を細分又は圧搾したものから抽出されたものであることが、本発明の組成物の品質向上及び製造安定性の観点から好ましい。このように、前記ハマミズナ科の植物体を、例えばミキサーなどを用いて、或いは手作業によるせん断力によって物理的に細分したか、又は機械を用いて或いは手作業などによって圧搾したものから抽出することによって、熱水抽出又はエタノール抽出などの場合のようにハマミズナ科の植物体に含まれる有効成分が変質、失活などしてしまう危険性を低減することができる。
<Extract>
The said extract is extracted from the plant body of the Amaranthaceae. The extraction method is not particularly limited and can be appropriately selected according to the purpose. However, the quality of the composition of the present invention is that it is extracted from a submerged or squeezed plant of the family Auriculariaceae. It is preferable from the viewpoint of improvement and production stability. In this way, the plant of the genus Sphagnum is extracted from what is physically subdivided by, for example, a mixer or by a manual shearing force, or squeezed by a machine or by manual labor. Therefore, the risk that the active ingredient contained in the plant body of the family A. thaliana will be altered or deactivated as in hot water extraction or ethanol extraction can be reduced.

また、前記ハマミズナ科の植物体をせん断力によって機械的乃至物理的に細分する際の細分時間としては、特に制限はなく、目的に応じて適宜選択することができるが、1時間以内であることが本発明の組成物の品質向上及び製造安定性の観点から好ましい。   In addition, the subdivision time for mechanically or physically subdividing the plant of the genus Phylumaceae is not particularly limited and can be appropriately selected according to the purpose, but is within one hour. Is preferable from the viewpoints of quality improvement and production stability of the composition of the present invention.

前記細分又は前記圧搾の際には他の溶媒(例えば水等)、助剤(例えば0.1質量%程度の微量のジエチレングリコール、アルコール等)などを加えてもよい。また、前記ハマミズナ科の植物体を手作業などによって圧搾する際には、細分化したハマミズナ科の植物体を、てこ、ネジ、水圧、油圧などを用いた圧搾機などで圧搾してもよい。このようにしても、ハマミズナ科の植物体を効率よく細分又は圧搾することが可能だからである。   In the subdivision or the squeezing, another solvent (for example, water or the like), an auxiliary agent (for example, a small amount of diethylene glycol or alcohol of about 0.1% by mass) or the like may be added. In addition, when squeezing the plant of the genus Sphagnum by hand, the subdivided plant of the sphagnum family may be squeezed with a press using a lever, a screw, hydraulic pressure, hydraulic pressure, or the like. Even if it does in this way, it is because it is possible to subdivide or squeeze efficiently the plant body of the genus Alaska.

前記ハマミズナ科の植物体を例えばミキサーなどを用いて或いは手作業によるせん断力によって物理的に細分、又は機械を用いて或いは手作業などによって圧搾した後に濾過する方法としては、特に制限はなく、任意の公知の濾過法を用いることができ、例えば濾布、フィルター、メンブレン、金属濾材、セラミック濾材などを好適に用いることができる。   There is no particular limitation as to the method of filtering the plant of the genus Occidentalis using a mixer or the like and physically subdividing it by a shearing force by hand or using a machine or by hand or the like. For example, filter cloths, filters, membranes, metal filter media, ceramic filter media and the like can be suitably used.

また、前記濾過する際には、圧力をかけて濾過を行ってもよい。前記圧力としては、特に制限はなく、目的に応じて適宜選択することができるが、0.1MPa〜10MPa程度の圧力で濾過を行うことが本発明の組成物の品質向上及び製造安定性の観点から好ましい。   Moreover, when filtering, you may filter by applying a pressure. There is no restriction | limiting in particular as said pressure, Although it can select suitably according to the objective, It is a viewpoint of the quality improvement of the composition of this invention, and manufacturing stability to perform filtration by the pressure of about 0.1 MPa-10 MPa. To preferred.

前記抽出物は、抽出した溶液のまま用いてもよく、必要に応じて、濃縮、希釈及び濾過処理、活性炭等による脱色、脱臭処理等をして用いてもよい。更には、抽出した溶液を濃縮乾固、噴霧乾燥、凍結乾燥等の処理を行い、乾燥物として用いてもよい。これらの中でも、凍結乾燥することが、抽出物の品質安定性及び取扱容易性の観点から好ましい。   The extract may be used as it is, or may be used after concentration, dilution and filtration treatment, decolorization with activated carbon, deodorization treatment, or the like, if necessary. Furthermore, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product. Among these, lyophilization is preferable from the viewpoint of the quality stability of the extract and ease of handling.

凍結乾燥時間としては、特に制限はなく、目的に応じて適宜選択することができるが、24時間以内であることが抽出物の品質安定性及び取扱容易性の観点から好ましい。   The lyophilization time is not particularly limited and may be appropriately selected depending on the intended purpose, but is preferably within 24 hours from the viewpoint of the quality stability of the extract and ease of handling.

前記抽出物を含有する組成物を調製する際には、前記抽出物をそのまま使用してもよく、単に水(精製水など)で希釈して用いてもよく、或いは抽出物の効果を損なわない範囲内で、本発明の組成物に用いられる成分である油脂類、ロウ類、炭化水素類、脂肪酸類、アルコール類、エステル類、界面活性剤、金属石鹸、pH調整剤、防腐剤、香料、粉体、紫外線吸収剤、増粘剤、色素、酸化防止剤、美白剤、キレート剤等の成分を配合することができる。   When preparing the composition containing the extract, the extract may be used as it is, or may be used by simply diluting with water (purified water or the like), or the effect of the extract is not impaired. Within the scope, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, perfumes, which are components used in the composition of the present invention, Components such as powders, ultraviolet absorbers, thickeners, pigments, antioxidants, whitening agents and chelating agents can be blended.

本発明の組成物に含まれる前記抽出物の含有量としては、特に制限はなく、目的に応じて適宜選択することができ、用いる剤型、使用対象等の様々の条件に応じて、広範囲でその含有量を適宜設定できる。なお、上述のように本発明の組成物は、前記抽出物そのもの(含有量:100質量%)でもよい。   There is no restriction | limiting in particular as content of the said extract contained in the composition of this invention, According to the objective, it can select suitably, According to various conditions, such as a dosage form to be used, a usage object, etc., it is in a wide range. The content can be set as appropriate. As described above, the composition of the present invention may be the extract itself (content: 100% by mass).

(化粧料)
本発明の化粧料は、前記組成物を含有してなり、必要に応じて更にその他の成分を含有してなる。この組成によれば、後述する実施例で示すように、優れたコラゲナーゼ活性阻害作用を有する組成物を含有するため、優れたシワ又はタルミの改善作用、皮膚のハリ改善作用、皮膚のキメ改善作用、皮膚の弾力性維持作用、美容作用、並びに抗老化作用を有する化粧料が得られる。
(Cosmetics)
The cosmetic of the present invention contains the above composition, and further contains other components as necessary. According to this composition, as shown in the examples to be described later, since it contains a composition having an excellent collagenase activity inhibitory action, it has an excellent wrinkle or tarmi improving action, a skin firmness improving action, and a skin texture improving action. Thus, a cosmetic having a skin elasticity maintaining effect, a cosmetic effect, and an anti-aging effect can be obtained.

前記化粧料は、前記組成物をそのまま使用してもよく、単に水(精製水など)で希釈して用いてもよく、或いは組成物の効果を損なわない範囲内で、組成物に用いられる成分である油脂類、ロウ類、炭化水素類、脂肪酸類、アルコール類、エステル類、界面活性剤、金属石鹸、pH調整剤、防腐剤、香料、保湿剤、粉体、紫外線吸収剤、増粘剤、色素、酸化防止剤、キレート剤等の成分を配合することができる。   The cosmetic may be used as it is, or may be used after simply diluted with water (purified water or the like), or within the range not impairing the effect of the composition. Oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, thickeners , Pigments, antioxidants, chelating agents, and other components can be blended.

本発明の化粧料は、外皮等に適用される化粧料であり、皮膚用、頭皮用、毛髪用、爪用、髭剃用、デオドラント用、香料用のいずれの用途にも用いることができる。
前記化粧料の剤型としては、特に制限はなく、アンプル状、カプセル状、粉末状、顆粒状、固形状、液状、ゲル状、気泡状、乳液状、クリーム状、軟膏状、シート状、ムース状、粉末分散状、多層状、エアゾール状等の剤型とすることができる。具体的には、化粧水、美容液、乳液、クリーム、軟膏、ローション、オイル、パック等の基礎化粧料;石鹸、クレンジングクリーム、クレンジングローション、クレンジングミルク、洗顔料等の皮膚洗浄料;シャンプー、リンス、トリートメント等の洗髪用化粧料や、ヘアクリーム、ヘアスプレー、ヘアトニック、ヘアジェル、ヘアローション、ヘアオイル、ヘアエッセンス、ヘアウォーター、ヘアワックス、ヘアフォーム等の整髪料、育毛・養毛料、1剤式染毛剤や2剤式染毛剤、ヘアカラー等の染毛料、パーマネントウェーブ剤や縮毛矯正剤等のパーマ剤やウエーブ保持剤等の頭髪化粧料、ファンデーション、白粉、おしろい、口紅、頬紅、アイシャドウ、アイライナー、マスカラ、眉墨、まつ毛等のメークアップ化粧料;美爪料等の仕上げ用化粧料;香水類、浴用剤、その他、歯磨き類、口中清涼剤、含嗽剤、ガム、キャンデー、タブレット等の口腔用組成物、抗炎症剤、腋臭防止剤、ふけ又はかゆみ予防用毛髪化粧料、脱毛抑制剤、除毛剤、髭剃り用剤、日焼け止め剤、にきび予防乃至改善剤、油性肌用化粧料、衛生用品、衛生綿類、ウエットティシュ等が挙げられる。
The cosmetic of the present invention is a cosmetic applied to the outer skin and the like, and can be used for any application for skin, scalp, hair, nail, shaving, deodorant, and fragrance.
The dosage form of the cosmetic is not particularly limited, and includes ampoules, capsules, powders, granules, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, mousses. , Powder dispersion, multilayer, aerosol and the like. Specifically, basic cosmetics such as skin lotion, cosmetic liquid, milky lotion, cream, ointment, lotion, oil, pack; skin cleansing agents such as soap, cleansing cream, cleansing lotion, cleansing milk, facial cleanser; shampoo, rinse , Hair-washing cosmetics such as treatments, hair creams, hair sprays, hair tonics, hair gels, hair lotions, hair oils, hair essences, hair water, hair wax, hair forms, hair straighteners, hair growth and hair restorations, 1 agent formula Hair dyes, two-component hair dyes, hair colorings such as hair colors, permanent hair preparations such as permanent wave agents and hair straighteners, and hair cosmetics such as wave retention agents, foundations, white powder, white hair, funny, lipsticks, blushers, Makeup cosmetics such as eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc .; Cosmetics for baldness; perfumes, bath preparations, other toothpastes, mouth fresheners, gargles, gums, candy, tablets and other oral compositions, anti-inflammatory agents, anti-odor agents, dandruff or itching hair makeup , Hair removal inhibitors, hair removal agents, shaving agents, sunscreen agents, acne preventing or improving agents, cosmetics for oily skin, hygiene products, sanitary cotton, wet tissue and the like.

本発明の化粧料に含まれる前記組成物の含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、品質向上及び製造安定性の観点から、乾燥質量として0.0001質量%〜50質量%が好ましく、0.01質量%〜10質量%が更に好ましいが、用いる剤型、使用対象等の様々の条件に応じて、広範囲でその含有量を適宜設定できる。   There is no restriction | limiting in particular as content of the said composition contained in the cosmetics of this invention, Although it can select suitably according to the objective, From a viewpoint of quality improvement and manufacturing stability, it is 0.0001 as dry mass. The content is preferably from 50% by mass to 50% by mass, and more preferably from 0.01% by mass to 10% by mass.

<飲食品>
本発明の組成物は、前記化粧料の他、飲食品に含有してもよい。この組成によれば、後述する実施例で示すように、優れたコラゲナーゼ活性阻害作用を有する組成物を含有するため、優れたコラゲナーゼ活性阻害作用、シワ又はタルミの改善作用、皮膚のハリ改善作用、皮膚のキメ改善作用、皮膚の弾力性維持作用、美容作用、及び抗老化作用を有する飲食品が得られる。
<Food &Drink>
You may contain the composition of this invention in food-drinks other than the said cosmetics. According to this composition, as shown in the examples described later, because it contains a composition having an excellent collagenase activity inhibitory action, it has an excellent collagenase activity inhibitory action, wrinkle or tarmi improving action, skin firmness improving action, A food / beverage product having a skin texture improving effect, a skin elasticity maintaining effect, a cosmetic effect, and an anti-aging effect is obtained.

前記飲食品は、前記組成物をそのまま使用してもよく、単に水(精製水など)で希釈して用いてもよく、或いは前記組成物の効果を損なわない範囲内で、種々の栄養成分を加えて、食用に適した形態、例えば、粉末状、粒状、顆粒状、液状、ペースト状、クリーム状、タブレット状、カプセル状、カプレット状、ソフトカプセル状、錠剤状、棒状、板状、ブロック状、丸薬状、固形状、ゲル状、ゼリー状、グミ状、ウエハース状、ビスケット状、飴状、チュアブル状、シロップ状、スティック状などに成形して食品素材として提供することができる。また、水、牛乳、豆乳、果汁飲料、乳清飲料、清涼飲料、青汁、ヨーグルトなどに添加して使用してもよい。   The food or drink may use the composition as it is, or may be used by simply diluting with water (purified water or the like), or various nutritional components within a range not impairing the effect of the composition. In addition, edible forms such as powder, granules, granules, liquids, pastes, creams, tablets, capsules, caplets, soft capsules, tablets, rods, plates, blocks, It can be formed into a pill shape, solid shape, gel shape, jelly shape, gummy shape, wafer shape, biscuit shape, bowl shape, chewable shape, syrup shape, stick shape, etc., and provided as a food material. Further, it may be used by adding to water, milk, soy milk, fruit juice drink, whey drink, soft drink, green juice, yogurt and the like.

前記飲食品に含まれる前記組成物の含有量としては、特に制限はなく、目的に応じて適宜選択することができるが、品質向上及び製造安定性の観点から、乾燥質量として0.0001質量%〜50質量%が好ましく、0.01質量%〜10質量%が更に好ましいが、用いる剤型、使用対象等の様々の条件に応じて、広範囲でその含有量を適宜設定できる。   There is no restriction | limiting in particular as content of the said composition contained in the said food-drinks, Although it can select suitably according to the objective, 0.0001 mass% as dry mass from a viewpoint of quality improvement and manufacturing stability. It is preferably ˜50% by mass, and more preferably 0.01% by mass to 10% by mass, but the content can be appropriately set in a wide range according to various conditions such as a dosage form to be used and a target to be used.

以下、本発明の実施例について説明する。なお、本発明は、下記の実施例に限定して解釈すべきではなく、特許請求の範囲における記載の範囲内で種々の変更が可能である。   Examples of the present invention will be described below. It should be noted that the present invention should not be construed as being limited to the following examples, and various modifications can be made within the scope of the claims.

<製造例1:抽出物1の調製>
4℃〜10℃で2時間以上冷蔵保存したアイスプラント(バラフ)の地上部を、室温(15℃〜25℃)下においてミキサーで20分間粉砕した。粉砕されたアイスプラント(バラフ)を濾布を用いて濾過し、濾液を凍結乾燥(18時間)させた。これを抽出物1とし、試験に供するまで−80℃で遮光保存した。
<Production Example 1: Preparation of Extract 1>
The above-ground part of the ice plant (barafu) refrigerated at 4 ° C. to 10 ° C. for 2 hours or more was pulverized with a mixer at room temperature (15 ° C. to 25 ° C.) for 20 minutes. The crushed ice plant (barafu) was filtered using a filter cloth, and the filtrate was freeze-dried (18 hours). This was designated as Extract 1 and stored at -80 ° C. in the dark until it was used for testing.

(実施例1:コラゲナーゼ活性阻害の評価試験)
アイスプラント(バラフ)から得られた抽出物のコラゲナーゼ活性阻害作用を、以下のようにして、試験した。
(Example 1: Evaluation test of collagenase activity inhibition)
The collagenase activity inhibitory action of the extract obtained from the ice plant (barafu) was tested as follows.

前記抽出物1を、Dulbecco’s Phosphate Buffered Saline(D−PBS(−)、タカラバイオ株式会社製)1錠を100mLの精製水に溶解させたD−PBS(−)溶液で希釈して得られたサンプル溶液を、0.2μmフィルターで濾過した。終濃度は、それぞれ1,500μg/mL、750μg/mL、375μg/mL、187.5μg/mL、93.8μg/mLとした。
コラゲナーゼB(ロッシュ社製、CatNo.1088 807)を0.1質量%BSA(ウシ血清アルブミン)含有PBS(−)溶液を用いて10μg/mLとなるように調製し、酵素溶液とした。ジメチルスルホキシド(DMSO)で溶解した蛍光基質(MOCAc−Pro−Leu−Gly−Leu−Apr(Dnp)−Ala−Arg−NH、Code 3163−v、ペプチド研究所製)を0.1質量%BSA含有PBS(−)溶液を用いて5μmol/Lとなるように調製し、蛍光基質溶液とした。
The extract 1 is obtained by diluting 1 tablet of Dulbecco's Phosphate Buffered Saline (D-PBS (-), manufactured by Takara Bio Inc.) in 100 mL of purified water. The sample solution was filtered through a 0.2 μm filter. The final concentrations were 1,500 μg / mL, 750 μg / mL, 375 μg / mL, 187.5 μg / mL, and 93.8 μg / mL, respectively.
Collagenase B (manufactured by Roche, Cat No. 1088 807) was prepared to a concentration of 10 μg / mL using a PBS (−) solution containing 0.1% by mass BSA (bovine serum albumin) to prepare an enzyme solution. Fluorescent substrate (MOCAc-Pro-Leu-Gly -Leu-A 2 pr (Dnp) -Ala-Arg-NH 2, Code 3163-v, manufactured by Peptide Institute) was 0.1 mass dissolved in dimethyl sulfoxide (DMSO) % BSA-containing PBS (−) solution was used to prepare a fluorescent substrate solution at 5 μmol / L.

<コラゲナーゼ活性阻害率の測定>
下記表1の処方に従い、各試料を準備した。具体的には、サンプルテスト用溶液の調製は次のようにして行った。96ウェルブラックプレートに各サンプル溶液を50μL/ウェルで添加した。その後、酵素溶液を100μL/ウェルで添加し、37℃で10分間インキュベートを行った。更に、蛍光基質溶液を50μL/ウェルで添加し、37℃で60分間インキュベートを行った後、分光光度計(サーモフィッシャーサイエンティフィック株式会社製)を用いて320nmで励起し405nmにおける蛍光強度を測定した。
<Measurement of collagenase activity inhibition rate>
Each sample was prepared according to the prescription in Table 1 below. Specifically, the sample test solution was prepared as follows. Each sample solution was added to a 96-well black plate at 50 μL / well. Thereafter, the enzyme solution was added at 100 μL / well and incubated at 37 ° C. for 10 minutes. Further, after adding a fluorescent substrate solution at 50 μL / well and incubating at 37 ° C. for 60 minutes, it was excited at 320 nm using a spectrophotometer (manufactured by Thermo Fisher Scientific Co., Ltd.), and the fluorescence intensity at 405 nm was measured. did.

前記サンプルテスト用試料の調製において、酵素溶液をD−PBS(−)に変更した試料をサンプルブランク用試料とした。また、前記サンプルテスト用試料及び前記サンプルブランク用試料において、サンプルを等量のD−PBS(−)に変更した試料をそれぞれ対照テスト用試料及び対照ブランク用試料とした。   In the preparation of the sample test sample, a sample blank sample was obtained by changing the enzyme solution to D-PBS (−). Moreover, in the sample test sample and the sample blank sample, samples obtained by changing the sample to an equal amount of D-PBS (−) were used as a control test sample and a control blank sample, respectively.

コラゲナーゼ活性阻害の測定は、次の式から求められる阻害率で表した。また、IC50の値は、希釈系列から近似曲線を作成して求めた。結果を図1及び表2に示す。
コラゲナーゼ活性阻害率(%)=[1−{(Sampletest−Sampleblank)/(Controltest−Controlblank)}]×100
Sampletest:サンプルテスト用試料の波長405nmにおける蛍光強度
Sampleblank:サンプルブランク用試料の波長405nmにおける蛍光強度
Controltest:対照テスト用試料の波長405nmにおける蛍光強度
Controlblank:対照ブランク用試料の波長405nmにおける蛍光強度
The measurement of collagenase activity inhibition was expressed by the inhibition rate obtained from the following formula. The IC 50 value was determined by preparing an approximate curve from the dilution series. The results are shown in FIG.
Collagenase activity inhibition rate (%) = [1-{(Sample test- Sample blank ) / (Control test- Control blank )}] × 100
Sample test : fluorescence intensity of the sample test sample at a wavelength of 405 nm Sample blank : fluorescence intensity of the sample blank sample at a wavelength of 405 nm Control test : fluorescence intensity of the control test sample at a wavelength of 405 nm Control blank : control blank sample at a wavelength of 405 nm Fluorescence intensity

図1及び表2から、アイスプラント(バラフ)の抽出物は、コラゲナーゼ活性阻害作用を有することが分かった。本発明の組成物は、このように高いコラゲナーゼ活性阻害作用を有するため、シワ又はタルミの改善、皮膚のハリ改善、皮膚のキメ改善、皮膚の弾力性維持、美容、抗老化の用途においても有用であると考察される。   From FIG. 1 and Table 2, it was found that the extract of ice plant (barafu) has a collagenase activity inhibitory action. Since the composition of the present invention has such a high collagenase activity inhibitory effect, it is also useful in applications such as improving wrinkles or tarmi, improving skin firmness, improving skin texture, maintaining skin elasticity, beauty, and anti-aging. It is considered that.

本発明の組成物は、ハマミズナ科の植物体から抽出した抽出物を含有することを特徴としており、優れたコラゲナーゼ活性阻害作用を有するため、コラゲナーゼ活性阻害用、シワ又はタルミの改善用、皮膚のハリ改善用、皮膚のキメ改善用、皮膚の弾力性維持用、美容用、及び抗老化用等の用途に有効である。本発明の組成物は、そのまま又は種々の成分を加えて、化粧料として好適に用いることができる。   The composition of the present invention is characterized in that it contains an extract extracted from a plant of the genus Phyllidae, and has an excellent collagenase activity inhibitory action. Therefore, it is used for inhibiting collagenase activity, for improving wrinkles or tarmi, It is effective for applications such as elasticity improvement, skin texture improvement, skin elasticity maintenance, cosmetics, and anti-aging. The composition of the present invention can be suitably used as a cosmetic as it is or with various components added.

Claims (4)

ハマミズナ科の植物体から抽出した抽出物を含有することを特徴とするコラゲナーゼ活性阻害用組成物。   A composition for inhibiting collagenase activity, which comprises an extract extracted from a plant of the family Occidentalis. ハマミズナ科の植物体から抽出した抽出物を含有し、抗老化、シワ又はタルミの改善、皮膚のハリ改善、皮膚のキメ改善、皮膚の弾力性維持、及び美容のいずれかの用途で用いられることを特徴とする組成物。   It contains an extract extracted from the plant body of the genus Camellia, and is used for any of the purposes of anti-aging, improvement of wrinkles or tarmi, improvement of skin firmness, improvement of skin texture, maintenance of skin elasticity, and beauty. A composition characterized by the above. ハマミズナ科の植物体が、アイスプラントに含まれる植物体である請求項1から2のいずれかに記載の組成物。   The composition according to any one of claims 1 to 2, wherein the plant body of the family Ciraceae is a plant body contained in an ice plant. 請求項1から3のいずれかに記載の組成物を含有することを特徴とする化粧料。   A cosmetic comprising the composition according to any one of claims 1 to 3.
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KR101574464B1 (en) 2015-02-02 2015-12-03 제주대학교 산학협력단 Natural dyeing with Tetragonia tetragonioides
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KR101574464B1 (en) 2015-02-02 2015-12-03 제주대학교 산학협력단 Natural dyeing with Tetragonia tetragonioides
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KR101930409B1 (en) * 2015-09-10 2018-12-18 김예주 Detergen composition for prevention of hair loss comprising iceplant as active ingredient

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