JPH01102011A - Skin cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin cosmetic, containing a fermentation product prepared by reacting a microorganism belonging to the genus Rhizopus with soybeans or a culture medium containing the soybeans and effective in preventing or treating liver-spot, ephelis, skin roughening, etc. CONSTITUTION:A skin cosmetic containing a fermentation product obtained by reacting a microorganism belonging to the genus Rhizopus, preferably Rhizopus.oligosporus, Rhizopus.oryzae, Rhizopus.arrhizus and/or Rhizopus.sto lonifer with soybeans (preferably processed or cooked soybeans or composition separated from soybeans) or a culture medium containing the soybeans, prefer ably fermentation produce, fermentation liquor or extract thereof, especially adjusted or processed so as to provide the fermentation product containing 4',6,7-trihydroxyisoflavone as an active ingredient, having the above-mentioned effect and capable of reviving the skin to a vivid state.

Description

Detailed Description of the Invention (Field of Industrial Application) This invention relates to new skin cosmetics (including medicinal cosmetics as quasi-drugs and external skin preparations as pharmaceuticals) that are effective in normalizing human skin. (same), that is, prevention of age spots, freckles, skin rashes, etc., relates to microbial preparations effective for treatment.

More specifically, the present invention relates to a skin cosmetic containing as an active ingredient a fermentation product obtained by allowing bacteria belonging to the genus Rhizopus to act on soybeans or a medium containing soybeans.

(Prior art and its problems) Conventionally, it has been proposed to incorporate various drugs into skin cosmetics for the prevention or treatment of age spots, freckles, flakes, etc. From this point of view, there are few that are fully satisfactory.

In recent years, vitamin C or its derivatives, aloe extract, placenta extract, glutathione, etc. have been used for this purpose, but these are still not satisfactory.

That is, vitamin C, placenta extract, and glutathione have problems with stability after preparation, and aloe extract has the disadvantage of browning over time.

(Progress of the Invention) As a result of intensive research to develop truly effective skin cosmetics, the inventor discovered that soybeans or a medium containing soybeans,
We discovered that a fermentation product obtained by treating bacteria belonging to the genus Rhizopus has an extremely effective effect on stains, freckles, skin rashes, etc., and based on this knowledge, we completed this invention. Ta.

Tempeh, a traditional fermented food in Indonesia, is a fermented product obtained by allowing bacteria belonging to the genus Rhizopus to act on soybeans. It is.

However, these are only used for food, and there are no examples of them being used as external preparations such as skin cosmetics.

The purpose of this invention is to provide a new material that is effective and safe for treating age spots, freckles, skin irritation, etc. by incorporating this fermentation product into skin cosmetics.

(Means for Achieving the Object) The present invention provides a skin cosmetic product characterized by containing a fermentation product obtained by allowing bacteria belonging to the Rhizobacterium to act on soybeans or a medium containing soybeans. This achieves the above objectives.

(Structure of the Invention) In this invention, the culture medium used when allowing the bacteria belonging to the genus Rhizopus to act is not particularly limited, but it must be one in which the bacteria used for carrying out the invention can propagate well, and must contain soybean or its components. Any medium may be used as long as it contains such a medium, and processed soybean products, cooked products, or compositions separated from soybeans are preferably used.

Usually boiled soybeans are used as is, but if necessary, soybean components such as soybeans or defatted soybeans or soymilk may be added to yeast extract, casein, whey protein, skim milk, protein decomposition products, various amino acids, inorganic nitrogen salts, etc. Nitrogen sources, carbon sources such as glucose, fructose, various starches, inorganic salts, vitamins, etc. can be added and used.

The method of fermentation by allowing bacteria belonging to the genus Rhizopus to act on a medium containing soybeans varies depending on the M type of the bacteria used and the type of medium, but the following methods are convenient and advantageous in practice: I can list some methods.

After carefully selecting and washing the soybeans, soak them in water to absorb enough water! At this time, bacteria may propagate, so add 0.0% to the soaking water.
Add lactic acid of about 1χ or use lactic acid bacteria such as Lactobacillus planklum (Lact
obacillus plantarum),
Lowering the pH can prevent the growth of bacteria.

After absorbing water, all the seed coats are removed from the soybeans and boiled at 100℃ for 1 hour.

After thoroughly draining the boiled soybeans, the spores of a fungus belonging to the genus Rhizopus are inoculated when the temperature has dropped to below 40°C.

A fermented product can be obtained by culturing in a fermentation chamber for 24 to 72 hours at a temperature of 30 to 40°C and a humidity of 70% or higher.

Any bacteria belonging to the genus Rhizorbus used in the fermentation of this invention may be used, and the fermentation product obtained by acting on soybeans or a medium containing soybeans can be used to treat stains, freckles, skin rashes, etc. The type of bacteria is not particularly limited as long as it has a preventive or therapeutic effect.

Preferably, any one of Rhizopus oligosporus, Rhizopus oryza, Rhizopus alizas, or Rhizopus stroniifa, which have been isolated and identified from the tempeh fermentation surface, or a combination thereof can be used. .

All of these bacterial species are known microorganisms, but the strains are not particularly limited, and those isolated from nature and their artificial or natural mutants can be used.

Furthermore, fungi belonging to the Rhizopus genus are parasitic on the leaves of broad-leaved trees such as bananas, teak, and laurel.
The leaves of these plants can also be dried in the shade and made into a powder, which can be used as a starter.

When actually using these fermented products, it is possible to use them as they are, but since they contain a large amount of insoluble solids such as soybeans, it is recommended to use fermented liquid or fermented liquid obtained by squeezing the fermented products. Solvent extracts or concentrates thereof may be used more effectively.

In the case of a squeezed fermentation liquid, since a large amount of the active ingredient may remain in the residue, it is more preferable to use it after extraction with an appropriate solvent.

The solvent to be used is not particularly limited as long as it is a solvent that can effectively extract components having a preventive or therapeutic effect on age spots, freckles, skin spots, etc. from the fermented product.

More preferably, water or ethanol or a mixture thereof may be used.

The fermented liquid or fermented extract obtained by allowing bacteria belonging to the genus Rhizopus to act on the thus obtained soybean or soybean-containing medium can be used alone as a skin cosmetic, but it may be used as a skin cosmetic. It can be used as a raw material for cosmetics by blending it into ordinary lotions, emulsions, creams, etc.

In this case, it is more preferable to use a concentrate or a dried product.

It has been found that the fermentation product thus obtained is extremely effective in preventing or treating age spots, freckles, skin rashes, etc., and rejuvenates the skin.

The active ingredient in this fermentation product and its mechanism of action are not yet clear and are currently under active research, but the following fact is considered to be one of the reasons.

That is, in the fermentation product obtained by the action of soybean Rhizopus succumbing bacteria, 4', which has not been detected in soybeans, is present.
, 6.7-1-lyhydroxyisoflavone is known to be detected.

This substance has a molecular formula of C11111@05 and a melting point of 319 (
decomp), UVJmax: 260.327 (m
/J), which has an extremely strong melanin production inhibitory effect in a melanin pigment production inhibition test, and is undoubtedly one of the important factors in its effectiveness against age spots, freckles, etc.

However, it is difficult to attribute the excellent effectiveness of the fermentation product obtained by treating soybeans with bacteria belonging to the genus Rhizopus solely to the presence of this substance.

The fermentation product contains many other unknown compounds, and these are thought to act additively or synergistically to exert the effect.

In addition, the fermentation product contains various amino acids, peptides, various sugars, various organic acids such as citric acid and pyroglutamic acid, various vitamins such as vitamin B6 and nicotinic acid, and various trace inorganic salts. This gives it more desirable properties as a skin cosmetic, such as prevention of flakes.

Therefore, when used in skin cosmetics, it is not necessarily necessary to identify and isolate the active ingredients.

Note that 4',6,7-trihydroxyisoflavone may be obtained by other means and incorporated into skin cosmetics.

(Effects of the Invention) This invention is a skin cosmetic containing a fermentation product obtained by allowing bacteria belonging to the genus Rhizopus to act on soybeans or a medium containing soybeans. It has an extremely effective effect on the prevention or treatment of skin rashes, etc., and can rejuvenate the skin.

Furthermore, this fermentation product contains various amino acids, peptides,
It contains various sugars, organic acids such as citric acid, vitamins, and various trace amounts of inorganic salts, and has an excellent moisturizing effect.Coupled with the above advantages, it is also an excellent ingredient in cosmetics. Has performance.

Next, this effect will be made clearer by describing embodiments and experimental examples of the present invention.

(Examples and Experimental Examples) Example 1 ~ (Preparation of extract of fermented product obtained by treating soybeans with Rhizopus oligosporus) 100 g of carefully selected soybeans
After washing thoroughly with water, immerse it in a 0.1x lactic acid aqueous solution to absorb enough water.

After absorbing water, all the seed coats are removed from the soybeans and boiled at 100°C for 1 hour.

Spread boiled soybeans quickly in a container and cool while removing as much water as possible.

When the temperature drops to 90°C, add about 1x of starch and mix well to gelatinize a portion.

When the temperature further drops to 40℃, add 3 ml to the defatted soybeans in advance.
Incubate at 7°C for 7 days, inoculate with Zopus oligosporus spores, mix well, and then let the mixture sit so that the temperature does not drop, and ferment for about 5 hours.

Next, the mixture is placed on a plastic tray to a thickness of about 2 cm, placed in a fermentation chamber, and cultured for 48 hours at a temperature of 37° C. and a humidity of 75%.

Approximately 180 g of lump-like fermented product was obtained.

This fermented product was quickly mixed well with 600 cc of a mixture of water and ethanol (9 parts water, 1 part ethanol) using a mixer, and then filtered under suction, and the residue was thoroughly washed with the mixed solution to obtain 1 l of a fermented product extract.

Furthermore, this filtrate was subjected to sterilization filtration using a membrane filter.

Example 2 (Preparation of extract of fermented product obtained by allowing Rhizopus oryza to act on soybeans) Example 1 Using Rhizopus oryza as a seed culture
Culture and extraction were performed in the same manner as above to obtain a fermented product extract.

However, the fermentation conditions were a fermentation chamber temperature of 30° C. and a fermentation time of 72 hours.

Example 3 (Preparation of extract of fermented product obtained by allowing Rhizopus alizas to act on soybeans) Example 2
Culture and extraction were performed in the same manner as above to obtain a fermented product extract.

Example 4 (Preparation of extract of fermented product obtained by treating soybeans with Rhizopus stoloniipha) Using Rhizopus stoloniifa as a seed culture and extraction in the same manner as in Example 2, a fermented product extract was prepared. I got it.

Example 5 (Preparation of l!11vI solution obtained by allowing Rhizopus oligosporus to act on a medium containing soybeans) To soy milk obtained from 100 g of carefully selected soybeans, 5 g of skim milk, glucose Ig, and 0.5 g of yeast extract were added. In addition, water is further added to prepare a liquid medium with a total volume of 11.

This is 21! The culture medium was then inoculated with Rhizopus oligosporus inoculum and cultured stationary at 30°C for 96 hours.

After suction filtration of this culture, the filtrate was further sterilized and filtered using a membrane filter to obtain about 950 cc of a fermentation liquid.

Experimental Example 1> (Melamine pigment production inhibition test of fermented product extract or fermentation liquid obtained in Example 1.2.3.4.5 and 4°, 6.7-trihydroxy inflavone) L-tyrosine (1,011g/cc) 0.5cc,
M/15 phosphate buffer (pH 6,8) 2.0cc, copper sulfate (lχ solution) 0.05cc, tyrosinase (1a
+g/cc) 1.0cc, Examples 1 and 2 as specimens
．． 2゜0cc of fermented product extract or fermented liquid obtained in 3.4.5
Or 4', 6.7-) Add 2.0cc of lyhydroxyisoflavone solution (0,0001χ) and mix.
Incubate for 30 minutes at 7°C, and test by spectrophotometry at 6°C.
Absorbance was measured at a wavelength of 70 nm.

Separately, the absorbance was determined using 2.0 cc of purified water instead of the sample as a blank, and by comparison with this,
The inhibition rate of melanin pigment production for each specimen was determined.

As shown in Table 1, these showed strong melanin pigment production inhibiting effects.

Table 1 Melanin pigment suppression test of the fermented product extract or fermentation liquid obtained in Example 1.2.3.4.5 and the 4°, 6.7-trihydroxyinflavone solution <Experimental Example 2> (Execution Effects of skin cosmetics containing the fermented product extract obtained in Example 1) Four types of lotions shown in Table 2 below were tested for their effects on age spots, freckles, and skin irritation as follows.

(1) Subjects: 30 girls with mini-stains (37-56
e) Freckles: 25 girls with freckles (18 years old and up)
42 years old) Hadaare: Girl 3 with subjectively and objectively recognized Hadaare symptoms
0 subjects (22 to 51 years old) (2) Test method For each symptom, subjects were divided into 5 groups, and the 4 groups were asked to apply lotion twice a day in the morning and evening after washing their faces with soap and warm water, and after 28 days and 56 days. The improvement effect was evaluated.

The remaining group served as a control.

(3) Evaluation stain 1: The color is quite dark and the border is clear.

2: The color is not very dark, but the border is quite clear.

3: The color is extremely pale and the border is not clear.

4 Symptoms of nijimi are not observed.

Freckles 1: Blackish spots spread across the cheeks, sometimes extending to the forehead.

2: Brown to grayish spots are spreading on the cheeks.

3: Light brown spots are observed on the cheeks, but they are not noticeable.

4. Symptoms of Nitzpacus are not observed.

Hadaare 1: The skin is dry and the dead skin cells come off easily, and when scratched with the tip of the toe, the dead skin cells come off easily. Always wiping white powder.

2: The skin is dry, the dead skin has peeled off in places, and there is a slight white powder.

3: My skin is dry, but there is no exfoliation or white powder - 4: My skin is moist and smooth.

The symptoms were evaluated as follows according to the classification of symptoms.

Effective: Symptoms improved from 1 to 3.4 and from 2 to 4.

Moderately effective: Symptoms 1 to 2, 2 to 3, and 3 to 4
improved on.

Ineffective: There is little change in symptoms or symptoms have worsened.

The evaluation results are shown in Tables 3 and 4.

As is clear from the results, all of the lotions containing the fermented product extract obtained in Example 1 have excellent effects.

Among them, the effect on skin rash was noticeable on the 28th day, and was fast-acting and long-lasting.

The effect on age spots was noticeable on the 56th day, and the effect was rather slow.

The effect on freckles appeared slightly on the 28th day, and 56
The effect is moderate even on the first day.

In comparison, in the control area, no effect was observed at all on age spots and freckles, and almost no effect was observed on skin spots.

(The following are blank spaces) Table 2 Table 3 Table 4 Experimental Example 3> (Preparation of cream containing the fermented product extract obtained in Example 1) Formula Weight % Stearic acid 8.0 Cetano 3.0 Beeswax
3.0 squalane
6.0 Octyldodecanol 6.0 Polyoxyethylene cetyl ether (251!, O,) 2.0
Lipophilic glyceryl monostearate 1.0 Preservative 0.2 Fragrance
0.5 Propylene glycol 3.0 Glycerin
3.0 borax
0.4 Fermented product extract obtained in Example 1
20.0 Purified water Remainder Cream prepared using the above formula for girls with visible stains 3
The product was used on 0 people (37-54 years old) (21 out of 30 had skin rash at the same time) and evaluated using the method used in Experimental Example 2, and the following evaluation results were obtained.

Effect on stains Effective on day 28 Effective on day 56? /30 19/30
Slightly effective 18/30 9/30 Ineffective 5/30 2/30 Effect on spider rash 28th day 56th day Effective 16/21 19/21 Slightly effective 4/21 2/21 Ineffective
Excellent effects were recognized as 1/21 and 0/21 or higher.

Claims (5)

[Claims] (1) A skin cosmetic product characterized by containing a fermentation product obtained by allowing bacteria belonging to the genus Rhizopus to act on soybeans or a medium containing soybeans. (2) The skin cosmetic according to claim 1, wherein the soybean is a processed soybean product, a cooked product, or a composition separated from soybean. (3) The fungus belonging to the genus Rhizopus is Rhizopus oligosporus.
rus), Rhizopus oryza
oryzae), Rhizopus
3. The skin cosmetic composition according to claim 1 or 2, characterized in that it is either Rhizopus arrhizus or Rhizopus stolonifer, or a combination thereof. (4) The skin cosmetic according to any one of claims 1 to 3, wherein the fermentation product is a fermented product, a fermented liquid, or an extract thereof. (5) Skin cosmetics according to claims 1 to 4, wherein the fermentation product is a fermented product that has been adjusted or processed to contain 4',6,7-trihydroxyisoflavone. fee.