KR101370113B1 - Cosmetics Compositions for Skin-lightening comprising Extracts of Eriobotrya japonica Lindl. Leaf - Google Patents

Abstract

The present invention relates to a whitening cosmetic composition containing an extract of Eriobotrya japonica Lindl. Leaf having skin whitening activity as an active ingredient, and this cosmetic composition has a whitening effect and specifically, solvent-extracted dry napa leaves 0.05 When cosmetics are produced in an amount of ˜20 parts by weight, it shows an excellent whitening effect such as suppressing melanin production.

Description

 A cosmetic composition for whitening containing bipar leaf extract as an active ingredient. Leaf}

The present invention relates to a whitening cosmetic composition containing an extract of Eriobotrya japonica Lindl. Leaf having an skin whitening activity as an active ingredient. The cosmetic composition has a whitening effect and specifically, solvent-extracted dry napa leaves 0.05. It relates to a cosmetic composition for whitening that exhibits a whitening effect such as suppressing melanin production when a cosmetic is made in an amount of ˜20 parts by weight.

As human needs become more diverse, the demand for white and clean skin is increasing day by day, and the market for whitening cosmetics is growing day by day, and the interest in its efficacy is growing, and as its functionality is emphasized, The tendency to obtain from friendly and natural ones is increasing and the demand for the use and research of natural raw materials is increasing.

The present invention relates to a cosmetic composition for whitening containing Eriobotrya japonica Lindl Leaf extract having the skin whitening activity as an active ingredient, so that the whitening effect on the napa leaf extract has not been reported so far, organic mixing such as ethanol It was confirmed through the inhibition of melanin production in the melanocytes of the extract as a solvent, it can be useful as a cosmetic or whitening agent for skin whitening because there is no cytotoxicity.

However, in the melanin synthesis process, the keratinocytes received ultraviolet light are signaled to melanocytes. It is converted into (DOPAquinone), and from the dopaquinone, a copolymer of melanin is produced through dopachrome through automatic oxidation reaction and enzyme reaction. Therefore, some whitening products have developed a component that has a whitening effect by controlling this signal transmission.

Prevention of pigmentation on the skin is mainly used for the step of inhibiting signal transmission to melanocytes by receiving ultraviolet rays, the component that inhibits tyrosinase activity, which is the main enzyme of melanin synthesis, and the substrate of tyrosinase. An antagonist is also developed, and a dopa reducing agent is developed to prevent oxidation of dopa, an intermediate metabolite of the melanin synthesis pathway.

Nowadays, arbutin, vitamin C derivatives and niacinamide are used in functional cosmetics, and many extracts such as ellagic acid are used as plant extracts, and hydroquinone is used as medicines, but it is used for skin side effects and safety problems. This is prohibited or used in extremely limited amounts. However, the above ingredients are limited in their use due to skin side effects, solubility, stability and the like. Vitamin C and its derivatives are difficult to use as raw materials for cosmetics due to their oxidation resistance, and arbutin is a derivative of glucopyranoside combined with hydroquinone. It is widely used as an ingredient for whitening, but it is reported that there is a risk of pruritus and the like.

On the other hand, loquat (Eriobotrya japonica Lindl.) Is a deciduous arborescent belonging to the family Rosaceae, reaching a height of 10m, small branches are rugged, strong and light brown, single leaves are regenerated, fruits are clustered, and flowering period is from September to October. The fruiting season is from May to June of the following year. It is cultivated for fruit or ornamental purposes in the southern province of Jeju Island, and the fruit of loquat is used for edible, sweet, sour and cold in nature.

As a related art related to loquat trees, shimizu et al. Revealed that loquat leaves have anti-inflammatory effects {Chem. Pharm. Bull. (Tokyo) 1986 Jun: 34 (6): 2614-7}, Jung et al. Reported the antioxidant activity of Methanol extracts from the leaves of the locust tree {Arch Pharm Res 1999 Apr: 22 (2). ): 213-8}. Shaw et al. Analyzed the organic acids and sugar components of the extracts of the loquat leaves using high pressure liquid chromatography. {J Sci Food Agrix 1981 Dec: 32 (12): 1242-6}, Godoy et al. Carotene content and vitamin A concentrations of loquat leaves were measured {Arch Latinoam Nutr 1995 Dec: 45 (4): 336-9}.

Currently, loquat leaves are widely used as a potted tea for drinking. According to Dongbogam, these loquat leaching liquids clear the lungs, act as antitussive expectorants, strengthen the stomach, and promote metabolism. (Agreement liquidation version).

As a prior art related to loquat, Korean Patent Publication No. 96-4026 describes a blood vessel suppressing composition containing ursolic acid obtained from loquat leaves, and Korean Patent Publication No. 92-8844 and Korean Patent Publication No. 2000-37680 Discloses a method for producing a bipa tea. However, it has not yet been found that loquat leaves have a beneficial effect on skin whitening that inhibits the deposition of skin pigments.

Therefore, the development of a whitening agent with excellent efficacy derived from nature is urgently required.

The inventors of the present invention, while studying natural medicinal plants for the whitening cosmetic composition, the extract extracted from the loquat leaf, which is a plant native to the high mountains of China, is safe for the skin, less discoloration and excellent melanin production inhibitory activity The present invention has been completed by revealing that it can be usefully used as a cosmetic composition for whitening.

Accordingly, an object of the present invention is to whitening cosmetics containing bifa leaf extract having a whitening activity that is safe, high solubility, easy to use, with little irritation to the skin to solve the problems of the prior art as described above as an active ingredient. It is to provide a composition.

The present invention provides a cosmetic composition for skin whitening, characterized in that it contains Ebibotrya japonica Lindl. Leaf extract as an active ingredient.

In addition, the cosmetic composition for skin whitening of the present invention is characterized in that the bifa leaf extract contains 0.05 to 20 parts by weight as an active ingredient based on the total weight of the composition.

In addition, the cosmetic composition for skin whitening of the present invention is characterized in that the bifa leaf extract is extracted with water, ethanol or a mixture thereof.

In addition, the cosmetic composition for skin whitening of the present invention is characterized in that the bipa leaf extract is extracted 1-2 times for 3 to 7 days at 30 ℃ to 50 ℃.

Finally, the cosmetic composition for skin whitening of the present invention is characterized by having a formulation selected from astringent cosmetics, softening cosmetics, creams, essences, foundations, soaps.

The whitening cosmetic composition containing the bipa leaf extract of the present invention as an active ingredient is effectively used in the whitening cosmetic composition because it suppresses excessive melanin production.

1 is a diagram showing the melanin production inhibitory by concentration of loquat extract compared with arbutin

   Hereinafter, the present invention will be described in detail.

The inventors of the present invention have no specific odor, no problems such as discoloration and discoloration, and at the same time, researching cosmetics having excellent whitening effect, confirming that the extract of Ebibotrya japonica Lindl. Leaf has excellent safety and excellent whitening effect. The invention was completed.

   Bipa leaf extract according to the present invention can be prepared as follows.

First, the loquat leaves are washed with water, and then dried in the shade. The dried loquat leaves are placed in an extraction container, and then the solvent is added with water, alcohol or a mixture thereof, and then extracted at a suitable temperature for a predetermined time. The extract is then filtered and concentrated.

It is preferable to use a solvent selected from water, alcohol or a mixed solvent thereof as the extraction solvent. Loquat dry leaves in the extraction to 10 to 50 parts by weight based on the solvent, preferably 10 to 20 parts by weight, less than 10 parts by weight of the excess solvent to use the economical and difficult to concentrate In case of more than 50 parts by weight, the loquat dried leaves are hardly sufficiently impregnated with the solvent.

The extraction method may be an extraction method such as heat extraction, reflux cooling extraction, ultrasonic extraction, and the like, and preferably may be extracted once or twice as an extraction method of the heat extraction, three or more times by reducing the active ingredient There is no meaning as a potent ingredient.

   The deposition temperature is 30 ℃ to 50 ℃, more preferably 45 ℃, the most effective in the prevention of discoloration and discoloration in this temperature range, below 30 ℃ takes a long extraction period, at 50 ℃ or more discoloration and There is a problem of malodor.

The immersion time is 3 to 7 days, preferably 5 days, less than 3 days the extraction of the desired component is insufficient, if more than 7 days there is no change in the extraction component and the extract discolors.

After obtaining the above-mentioned loquat leaf extract, the solids are removed using a filter paper or the like, filtered through a 0.2-0.5 μm filter, and the supernatant is filtered under reduced pressure. As described above, the loquat leaf extract may be provided as an extract having an excellent melanin production inhibitory activity.

In addition, the bipa leaf extract of the present invention provides a cosmetic composition for skin whitening containing 0.05 to 20 parts by weight based on the total weight of the composition as an active ingredient. At this time, when the content of the bipa leaf extract is less than 0.05 parts by weight relative to the total weight of the composition, it is difficult to achieve the expected efficacy, and when it exceeds 20 parts by weight, it is difficult to expect an increase in additional efficacy.

Finally, the present invention is a converging lotion, flexible lotion, cream, nutrition essence, foundation, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, prepared from cosmetic composition for skin whitening containing bifa leaf extract as an active ingredient, Provided is a cosmetic composition for skin whitening comprising a formulation such as a body lotion, a body cleanser, an emulsion, and the like.

The present invention having the configuration as described above may be better understood by the following examples, the examples are for illustrative purposes of the present invention and the present invention is not limited by these examples.

 Preparation of Loquat Leaf Extract for Skin Whitening

Bibi leaf (Eriobotrya japonica Lindl. Leaf) of the present embodiment was used in Korea, and washed the collected bipa leaf to remove foreign matters and dried. 100 g of the dried plant was immersed in 1000 ml of solvent (ethanol: purified water = 75: 25) and leached in a 45 ° C. thermostat for 5 days. The obtained extract was cooled to room temperature, filtered through a strainer, the filtrate was collected, and the filtrate was concentrated with a rotary vacuum concentrator to obtain 200 g of a loquat leaf extract.

Test Example 1 Primary stimulation test on human body (closed cloth test)

   In order to determine the safety of the loquat extract used in the cosmetic composition for skin whitening of the present invention to the human body, the stimulation test was performed through a closed patch test as follows. Subjects selected 30 adults and the test site was forearm.

After making samples with each concentration (control (distilled water), loquat extract 0.1%, loquat extract 0.5%, loquat extract 5%}), 15 µl of the sample was added dropwise to the fin chamber and then placed on the upper part of the test site. Put on and fix with test tape. After 24 hours, the sample was removed, and then the skin reaction was determined after 4 hours with respect to the indication of the test site. The criterion is as shown in [Table 1], and the result is shown in [Table 2].

Criteria -  No unusual phenomenon ±  Fine erythema +  Erythema +  Erythema abnormalities, rashes

In addition, the stimulus degree, which is one of the determination results, is calculated according to the following equation.

Stimulus = {(-x) + (± x) + (+ x) + (++ x)} / Number of Subjects

Judgment result sample - ± + + Irritation degree Control (Distilled Water) 27 2 One - 0.1 Loquat extract 0.1% 28 One - One 0.1 Loquat extract 0.5% 30 - - - 0 Loquat extract 5% 25 3 - 2 0.3

Looking at the result of the determination, it can be seen that the irritation degree is finely increased as the content is higher than the control, but it can be seen that there is almost no skin reaction at a certain content. Therefore, it can be seen that the cosmetic composition for skin whitening of the present invention is suitable as a raw material of the external preparation for skin.

Test Example 2 Measurement of Melanin Inhibition

 <1-1> Culture of B16 melanoma cells

   B16 melanoma cells were purchased from ATCC (The American Type Culture Collection), and the above test method was used to measure the degree of inhibition of melanin synthesis by sample treatment when cultured in B16 melanoma cells.

The sample used in the test was prepared by dissolving the non-leaf extract in Dimethyl Sulfoxide (DMSO), and the sample concentration was prepared by diluting 1000 times the concentration to be treated in the cells. Forskoline (Fk) used in the test was melanin through the cAMP route. As a representative substance that induces production, it is used to treat the whitening candidate material with the whitening candidate material to see if it significantly blocks the production of melanin induced by Fk. The Fk used for the test was prepared by dissolving in DMSO at a concentration of 2 mM.

To culture the cells, B16 melanoma cells were added to Dulbeco "s Modified Eagle" s Medium (DMEM) medium with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37 ° C and 5% CO ₂ . The sample was treated under the following conditions.

1) B16 melanoma cells were divided into 6 wall plates at ⁴ × 10 ⁴ each.

   2) After the cells were attached, the test substances were treated by concentration, and then treated with Fk (2 μM), which is known as a melanin synthesis inducer, and cultured for 72 hours.

3) After 72 hours, the cells were washed twice with Phosphate buffered sallne (PBS) and then recovered by treatment with trypsin. The recovered cells were centrifuged at 5000 g for 10 minutes to remove the supernatant, and then cell pellets were obtained.

<1-2> Protein quantification test

   The total protein content of the recovered cells was measured using a Bio-Rad protein assay kit.

   1) After adding the protein sxtraction brffer to the recovered cell pellet, loosen the pellet well and react for 40 minutes on ice.

   2) After the cell lysis, centrifuge at 12,000rpm for 10 minutes to recover the supernatant, dilute it appropriately, add the protein assay kit solution and react for 15 minutes at room temperature. At this time, bovine albumin serum (BAS) was used as a standard.

   3) After the reaction is completed, absorbance is measured at 750 nm using an ELISA reader (BioTek Inc.).

4) Insert the measured value into the standard curve to find the amount of protein.

<1-3> Melanin synthesis inhibition test by absorbance measurement

   In order to measure the whitening effect of bifa leaf extract, the test material was treated to measure the amount of melanin in cultured cells to determine whether melanin synthesis was inhibited by the sample treatment.

   1) After protein determination of the recovered cells is completed, they are reacted at 60 ° C for 30 minutes to obtain intracellular melanin.

   2) After completion of the reaction, the absorbance was measured at 490 nm using an ELISA reader (Bio-tek Inc.).

   3) Draw a standard curve of synthetic melanin (Sigma Aldrich. USA) at a concentration of 0 to 0.25 mg / mL, and calculate the amount of melanin by substituting absorbance values obtained from the sample.

   The melanin inhibition rate of each measured sample can be calculated by the following formula from the melanin production, the results are shown in the following [Table 3] and [FIG. 1].

    Melanin Formation (%) = Treatment Absorbance / No Treatment Absorbance × 100

% Melanin inhibition = 100-melanin production

 Effect of Loquat Leaf Extract on Melanin Synthesis in B16 melanoma Cells Name of sample Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 No treatment Arbutin Melanin synthesis
Inhibition rate (%) 30.0 35.4 39.1 48.0 37.3 33.7 8.0 32.0

Note 1: Sample 1: 0.001 parts by weight of non-leaf extract, Sample 2: 0.05 parts by weight of non-leaf extract

     Sample 3: 0.2 parts by weight of non-leaf extract, Sample 4: 1.0 parts by weight of non-leaf extract

Sample 5: 20.0 parts by weight non-leaf extract Sample 6: 21.0 parts by weight non-leaf extract

   As can be seen from the result of [Table 3], when the bipa leaf extract is contained within the range of 0.05 parts by weight to 20 parts by weight, the sample 1 and the sample 6 outside the above range, compared with the non-treated, arbutin when compared to Since it suppresses excessive melanin production, it can be effectively used in the cosmetic composition for whitening.

Cosmetics prepared with a cosmetic composition containing the bipa leaf extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the non-leaf extract of the present invention by containing a dermatologically acceptable medium or base can be prepared in the form of adjuvants that can be topically or systemically applied commonly used in the field of dermatology.

   Cosmetic formulations are, for example, in the form of emulsions, suspensions, microemulsions, creams, skins, lotions, powders, ointments, sprays or conceal sticks obtained by dispersing an oil phase in a solution, gel, solid or water phase. Can be provided.

In addition, the cosmetic composition of the present invention, in addition to napa leaf extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Production Example

Preparation Example 1

Convergence Cosmetics ingredient content(%) function  1,3-butylene glycol 4.0  Moisturizer  Polyoxyethylene (60) hardening castor oil 0.5  Surfactants  glycerin 2.0  Moisturizer  Preservative 0.15  Preservative  Spices 0.1  air freshener  ethanol 4.0  Barrier strengthening agent  Sodium hyaruronate 0.2  Skin protection  EDTA-2Na 0.02  Skin Conditioning Agent  Allantoin 0.1  Skin protection  Purified water Remainder  Skin Conditioning Agent  Loquat Leaf Extract 0.3  Whitening agent

Preparation Example 2

Flexible lotion ingredient content(%) function  1,3-butylene glycol 4.0  Moisturizer  Cetostearyl alcohol 0.1  Emollients  Polyoxyethylene lauryl ether 0.5  Surfactants  Monostearyl polyoxyethylene sorbitan 1.5  glycerin 5.0  Moisturizer  Glyceryl monostearate 1.5  Emollients  Preservative 0.15  Preservative  Sunflower oil 8.0  Emollients  Spices 0.1  air freshener  Loquat Leaf Extract 0.5  Barrier Enhancer  cholesterol 0.5  Skin protection  Ceramide 0.2  Stearic acid 0.8  Skin Conditioning Agent  KOH 0.05  corrector  Carbomer 0.1  Incrementer  Purified water Remainder  Skin Conditioning Agent

Preparation Example 3

essence ingredient content(%) function  1,3-butylene glycol 4.0  Moisturizer  Polyoxyethylene lauryl ether 0.5  Surfactants  Monostearyl polyoxyethylene sorbitan 1.5  glycerin 15.0  Moisturizer  Preservative 0.15  Preservative  Squalane 3.0  Emollients  Spices 0.1  air freshener  Loquat Leaf Extract 0.5  Barrier Enhancer  Ceramide 0.2  Skin protection  Beta Glucan 1.0  Skin protection  KOH 0.2  corrector  Carbomer 0.2  Incrementer Hyaluronic acid (1.0%) 5.0  Skin protection  Purified water Remainder  Skin Conditioning Agent

Production Example 4

cream ingredient content(%) function  Squalane 5.0  Emollients  vaseline 2.0  Emollients  Self-emulsifying glycerin monostearate 2.5  Ancillary surfactant  glycerin 8.0  Moisturizer  Spices Suitable amount  air freshener  Preservative  Preservative  Liquid paraffin 3.0  Emollients  beeswax 1.0  Microstellin wax 1.0  Skin protection  Cetearyl alcohol 2.0  Emollients  Glyceryl stearate 2.0  Loquat Leaf Extract 1.0  Barrier Enhancer  Cetearylglucoside 2.0  Surfactants  Ceramide 0.3  Skin protection  Stearic acid 1.0  Skin Conditioning Agent  KOH 0.3  corrector  Magnesium aluminum silicate 0.4  Thickener

Claims (5)

In the cosmetic composition for skin whitening containing Eriobotrya japonica Lindl. Leaf extract as an active ingredient,
The bipa leaf extract contains 0.05 to 20 parts by weight as an active ingredient based on the total weight of the cosmetic composition for skin whitening, and is extracted with water, ethanol or a mixture thereof at a temperature of 45 ° C. for 1 to 2 times for 5 days. Whitening cosmetic composition.
delete delete delete The method of claim 1,
The cosmetic composition for skin whitening is characterized in that it has a formulation selected from astringent makeup, softening cream, cream, nutrient essence, foundation, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion Cosmetic composition for skin whitening.

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