CN114209744A - Cream for treating mastitis of dairy cows and preparation method thereof - Google Patents

Cream for treating mastitis of dairy cows and preparation method thereof Download PDF

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CN114209744A
CN114209744A CN202210026141.5A CN202210026141A CN114209744A CN 114209744 A CN114209744 A CN 114209744A CN 202210026141 A CN202210026141 A CN 202210026141A CN 114209744 A CN114209744 A CN 114209744A
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raspberry
accounts
cream
mastitis
extract
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CN114209744B (en
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王云生
薛挺
张妞妞
汪晖
钱薇
周祺芳
周欣月
钱一萱
汪岳
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Anhui Agricultural University AHAU
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/194—Carboxylic acids, e.g. valproic acid having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0041—Mammary glands, e.g. breasts, udder; Intramammary administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Abstract

The invention provides a cream for treating mastitis of a dairy cow, and belongs to the technical field of treatment of mastitis of dairy cows. The cream for treating the mastitis of the dairy cows comprises a raspberry leaf extract, wherein the preparation method of the raspberry leaf extract comprises the steps of drying and crushing the raspberry leaves, mixing the crushed raspberry leaves with tartaric acid, adding an ethanol solution for extraction, filtering insoluble substances after the extraction is finished, concentrating, extracting with ethyl acetate for decoloration, and extracting an extracted water phase with n-butyl alcohol. And finally, performing rotary evaporation on the n-butanol phase to obtain a solid substance, namely the raspberry leaf extract. The cream can quickly cure mastitis of dairy cows which can not be cured by antibiotics, inhibits multidrug-resistant staphylococcus aureus for a long time, does not find the situation of drug resistance after long-time tracking, and is obviously better than the existing antibiotic products on dairy cows with recurrent mastitis.

Description

Cream for treating mastitis of dairy cows and preparation method thereof
Technical Field
The invention relates to the technical field of treatment of cow mastitis, and particularly relates to a cream for treating cow mastitis and a preparation method thereof.
Background
Mastitis is the most common and expensive disease of cows. It affects the health of cows and causes economic losses by reducing milk production, reducing milk quality, premature killing, veterinary treatment and treatment with antibiotics. Statistically, about one third of the cows worldwide suffer from various types of mastitis, and worldwide losses due to mastitis amount to several billion dollars each year. Staphylococcus aureus is a main pathogenic bacterium causing cow mastitis, antibiotics are mainly used for treating cow mastitis at present, but long-term use of the antibiotics causes the drug resistance of cow-derived staphylococcus aureus to be more serious.
Rubi fructus is an ancient Chinese medicinal material, and is dried fruit of Rubus chingii Ching of Rosaceae. Is distributed in Jiangsu, Zhejiang, Anhui, Fujian, Jiangxi, Guangxi, Guizhou and other places. Has the functions of invigorating kidney, stopping nocturnal emission, reducing urination, nourishing liver and improving eyesight. It is commonly used for seminal emission, spermatorrhea, enuresis, frequent micturition, impotence, premature ejaculation, dim eyesight and dim flower. The compendium of materia medica records that the raspberry is a medicine for nourishing true yin, has slightly sour flavor and can collect and control dissipated yin qi to generate essence liquid, so the kouzong 22893 is called tonifying kidney and reducing urine, and the drowning device is covered when the raspberry is taken, and the raspberry is reasonable even if the raspberry is attached to the drowning device. In Benjing, the five zang organs are mainly regulated, and yin is also the zang organ. All herbs are firm, can tonify middle energizer, and can tonify yin of five zang organs to replenish essence and qi. All herbs are heavy and can tonify kidney, and they are specialized in kidney yin to strengthen kidney qi and strengthen will and strengthen strength, so they are also effective in tonifying kidney yin.
The raspberry is rattan shrub with a height of 1.5-3 m; thin branches with skin pricks and no hairs. Single leaf, nearly circular, diameter 4-9 cm, with vellus or few vellus along the veins on both sides, heart-shaped base, 5 deep cleft palm-shaped edge, 3 or 7 thin clefts, oval or rhomboid oval cleft, tapered tip, narrow contracted base, nearly equal length or slightly longer top and side clefts, heavy sawtooth, 5 veins on palm; the leaf stalk is 2-4 cm long, has little or no hair, and has small skin prick; the shape of the supporting leaves is like a needle. Single flower axilla is grown, and the diameter is 2.5-4 cm; the flower stalk is 2-3.5(4) cm long and has no hair; the calyx barrel hair is more dilute or nearly hairless; the sepal is oval or egg-shaped and long round, the top end is provided with a convex tip, and the outside is densely covered with short and soft hair; the petals are oval or oval and are white, the top ends of the petals are round and blunt, the length of the petals is 1-1.5 cm, and the width of the petals is 0.7-1.2 cm; most stamens and filaments are wide and flat; most pistils have soft hair. The fruits are nearly spherical, red, 1.5-2 cm in diameter and dense, grey and soft; the nucleus had wrinkles. The flowering period is 3-4 months, and the fruit period is 5-6 months.
The applicant extracts and purifies the effective components in the leaves of the raspberry to obtain the raspberry leaf extract, and researches various activities of the raspberry leaf extract to find that the raspberry extract has a very good bacteriostatic action on staphylococcus aureus and is matched with synergistic auxiliary materials, so that the obtained composition can be used for quickly treating mastitis of dairy cows.
Disclosure of Invention
The invention aims to provide cream for treating bovine mastitis, which comprises a raspberry leaf extract, wherein the preparation method of the raspberry leaf extract comprises the steps of drying and crushing raspberry leaves, mixing the crushed raspberry leaves with tartaric acid, adding an ethanol solution with the volume fraction of 50-75%, extracting for 10-120 min at the temperature of 60-65 ℃, filtering out insoluble substances after extraction, concentrating, extracting with ethyl acetate for decolorization, and extracting the extracted water phase with n-butyl alcohol. And finally, performing rotary evaporation on the n-butanol phase to obtain a solid substance, namely the raspberry leaf extract.
Wherein the cream for treating the mastitis of the dairy cattle comprises the components of raspberry leaf extract, ethylene diamine tetraacetic acid, vitamin C, glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol; wherein the water phase comprises raspberry leaf extract, tartaric acid, disodium ethylene diamine tetraacetate and vitamin C, wherein the raspberry leaf extract accounts for 1-10%, the tartaric acid accounts for 1-3%, the disodium ethylene diamine tetraacetate accounts for 0.1-0.5% and the vitamin C accounts for 0.1-0.5%; the oil phase comprises 15-30% of glycerol, 20-30% of white vaseline, 30-40% of liquid paraffin, 3-6% of salicylic acid, 2-5% of vitamin E, 2-5% of ethylparaben, 1-4% of glyceryl monostearate and 1-3% of hexadecanol; the mixing ratio of the oil phase to the water phase is 6: 1-2: 1.
The mass volume ratio of the crushed raspberry leaves to the ethanol water solution is 40-60 g/L, and the mass volume ratio of the tartaric acid to the ethanol water solution is 2.667-4 g/L.
The mass volume ratio of the raspberry leaf powder to pure water is 166.67-300 g/L, and the mass volume ratio of tartaric acid to pure water is 6.667-20 g/L.
Meanwhile, the invention also provides a preparation method of the cream for treating the mastitis of the dairy cattle, which comprises the following specific steps:
drying the raspberry leaves, crushing the raspberry leaves into powder, adding 50-75% ethanol solution by volume fraction, performing ultrasonic-assisted extraction at the temperature of 60-65 ℃, filtering insoluble substances after extraction, concentrating, extracting and decoloring with ethyl acetate, and extracting the extracted water phase with n-butyl alcohol. And finally, performing rotary evaporation on the n-butanol phase to obtain a solid substance, namely the raspberry leaf extract.
Dissolving the extract of the raspberry leaves in aqueous solution of tartaric acid according to a formula, adding disodium ethylene diamine tetraacetate and vitamin C after the extract is completely dissolved, and obtaining treated extract of the raspberry leaves; wherein, the extract of the raspberry leaves accounts for 1-10%, the tartaric acid accounts for 1-3%, the disodium edetate accounts for 0.1-0.5%, and the vitamin C accounts for 0.1-0.5%.
Mixing glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol to obtain a mixed emulsion; wherein, the glycerin accounts for 15-30%, the white vaseline accounts for 20-30%, the liquid paraffin accounts for 30-40%, the salicylic acid accounts for 3-6%, the vitamin E accounts for 2-5%, the ethylparaben accounts for 2-5%, the glyceryl monostearate accounts for 1-4%, and the cetyl alcohol accounts for 1-3%.
Mixing the treated extract of the cotyledon of the raspberry with the mixed emulsion according to the proportion of 1:4, and emulsifying at the temperature of 35-50 ℃ to obtain the cream for treating the mastitis of the dairy cows.
Advantageous effects
The research of the research subject group finds that substances extracted by ethanol after the raspberry leaves and the tartaric acid are mixed have strong bacteriostasis to staphylococcus aureus and propionibacterium acnes, the bacteriostasis component has good bacteriostasis effect under the action of the tartaric acid, and the analysis reason may be that certain groups of the tartaric acid and flavonoid or polyphenol substances in the raspberry leaves have complexation or synergism, so that the raspberry leaves generate a strong bacteriostasis substance.
The extract of the cotyledon of the raspberry has good antibacterial effect, and particularly has good antibacterial effect on gram-positive bacteria such as propionibacterium acnes and staphylococcus aureus. The result shows that the antibacterial activity of the extract of the raspberry leaf is stronger than that of antibiotics, so that the applicant prepares the cream for treating the mastitis of the milk cow, the cream for treating the mastitis of the milk cow has better emulsion stability, the extract of the raspberry leaf serving as the antibacterial component can be kept on the skin of the milk cow and absorbed for a long time, the long-time antibacterial effect is achieved, the mastitis of the milk cow caused by staphylococcus aureus is eradicated, the cure effect is very good, experiments show that the cream for treating the mastitis of the milk cow is coated on the skin around the mammary gland of the milk cow for 2 times after 24 hours, the cream is continuously coated for 2-6 days after 12 hours of coating every time, the mastitis of the milk cow completely disappears, and the mastitis of the milk cow which can not be cured after 30-time administration of the antibiotics can be rapidly cured by the cream prepared by the invention, and after long-time tracking, the situation of drug resistance is not found, and the antibiotic product is obviously better than the existing antibiotic product on the dairy cattle with repeated mastitis.
Drawings
FIG. 1 is a graph of the effect of raspberry leaf extract on the growth curve of a bovine-derived Staphylococcus aureus;
FIG. 2 is a graph showing the effect of ethanol extract of Rubus idaeus on the biofilm of Staphylococcus aureus SA 2;
FIG. 3 is a graph of the effect of ethanol extract of raspberry leaves on the biofilm of Staphylococcus aureus SA 3;
FIG. 4 is a graph of the effect of aqueous extract of raspberry cotyledons on the biofilm of Staphylococcus aureus SA 2;
Detailed Description
The dairy cow-derived staphylococcus aureus strains SA2 and SA3 used in the embodiment are multiple drug-resistant strains and generally resistant to antibiotics such as penicillins and erythromycins.
Example 1
This example is a bacteriostatic test of extract of cotyledons of raspberry.
Drying the raspberry leaves, smashing the raspberry leaves into powder, weighing 30g of leaves and 2g of tartaric acid, adding 750mL of ethanol solution with the volume fraction of 60%, carrying out ultrasonic assisted extraction for 30min at the temperature of 60-65 ℃ by using 200w of power, filtering out insoluble substances after extraction is finished, carrying out rotary evaporation concentration on filtrate, extracting and decoloring the filtrate to 20mL by using equal-volume ethyl acetate, and extracting the extracted water phase by using equal-volume n-butyl alcohol. And finally, carrying out rotary evaporation on the n-butanol phase to obtain a solid substance, thus obtaining the raspberry leaf extract.
Determining OD of Staphylococcus aureus cultured for 12h by two-fold dilution method600Post determination of OD6001, 1 again: 100 are respectively diluted in 10 EP tubes, 20mg/mL of raspberry leaf extract or antibiotics are added into a first tube, the first tube is fully and uniformly mixed, 500uL of the mixture is absorbed and transferred to a second tube, the rest is carried out until the ninth tube and the tenth tube are used as controls, the fifth tube and the tenth tube are placed in a constant temperature incubator for standing and culturing for 24 hours, and the experimental result is observed. The experiment was repeated three times.
TABLE 1 minimum inhibitory concentration of extract of cotyledon of Rubi fructus and penicillin antibiotic against Staphylococcus aureus
Figure BDA0003463985800000041
TABLE 24 MIC (mg/mL) result interpretation standards for antimicrobial agents
Interpretation standards Amp PEN OXA Amo
Drug resistance (R) ≥32 ≥32 ≥4 ≥32
Moderately sensitive (I) 16 16 3 16
Sensitive (S) ≤8 ≤8 ≤2 ≤8
And (4) analyzing results: from table 1 table 2 it can be derived: the staphylococcus aureus of milk origin is resistant to all four penicillin antibiotics. Compared with penicillin antibiotics, the raspberry leaf alcohol extract has the antibacterial effect obviously superior to the four antibiotics, and the water extract has the antibacterial effect lower than the four antibiotics but also has a certain antibacterial effect.
Example 2
Drying the raspberry leaves, smashing the raspberry leaves into powder, weighing 30g of leaves and 2g of tartaric acid, adding 750mL of 75% ethanol solution by volume fraction, carrying out ultrasonic assisted extraction at the temperature of 60-65 ℃ for 60min by using 150w of power, filtering insoluble substances after extraction is finished, carrying out rotary evaporation concentration on filtrate, extracting and decoloring the filtrate to 20mL by using equal-volume ethyl acetate, and extracting the extracted water phase by using equal-volume n-butyl alcohol. And finally, carrying out rotary evaporation on the n-butanol phase to obtain a solid substance, thus obtaining the raspberry leaf extract.
Determining growth curve of Staphylococcus aureus by using the extracted folium Rubi extract, and performing three passages of cow-derived Staphylococcus aureus by OD600Initial value of 0.05 transferred to 50mL TSB, OD measured every 2 hours600The experimental results obtained are shown in FIG. 1: the left graph in fig. 1 is the growth curve of the raspberry leaf extract prepared in this example on the staphylococcus aureus of dairy cow source, and the right graph in fig. 1 is the effect on the CFU of the staphylococcus aureus of dairy cow source, and it can be seen from the graph: the Rubi fructus extract can effectively inhibit the two kinds of bacteria even under rich liquid culture medium conditionAnd (4) growing the staphylococcus aureus. Ethanol as a control group showed: it only can delay the growth of the staphylococcus aureus of milk cow source and can not cause the inhibition effect of the bacterial strain.
FIG. 2 is a graph showing the effect of ethanol extract of Rubus idaeus on the biofilm of Staphylococcus aureus SA 2; as shown in FIG. 2, the ethanol extract of raspberry leaves has a good inhibitory effect on SA 2. When the concentration of the ethanol extract of the raspberry leaves reaches 66.08ug/mL, the inhibition rate of the ethanol extract on the staphylococcus aureus SA2 biofilm reaches 92.3%, and the inhibition effect is obvious. And the inhibition effect of the antibiotic Amp has no obvious change under the condition of the same concentration.
FIG. 3 is a graph of the effect of ethanol extract of raspberry leaves on the biofilm of Staphylococcus aureus SA 3; as can be seen from FIG. 3, the ethanol extract of raspberry leaves has a good inhibitory effect on SA 3. When the concentration of the ethanol extract of the raspberry leaves is 66.08ug/mL, the inhibition rate of the ethanol extract on the staphylococcus aureus SA3 biofilm reaches 84.7%, and the inhibition effect is obvious. And the inhibition effect of the antibiotic Amp has no obvious change under the condition of the same concentration.
FIG. 4 is a graph of the effect of aqueous extract of raspberry cotyledons on the biofilm of Staphylococcus aureus SA 2; as can be seen from FIG. 4, the aqueous extract of raspberry leaves had a certain inhibitory effect against SA 2. When the concentration of the added raspberry cotyledon reaches 871.19ug/mL, the inhibition rate of the raspberry to the staphylococcus aureus SA2 biofilm reaches 88.76%, and the inhibition effect is obvious. And the inhibition effect of the antibiotic Amp has no obvious change under the condition of the same concentration.
Example 3
The embodiment provides a cream for treating mastitis of dairy cows, which mainly comprises raspberry leaf extract, tartaric acid, disodium ethylene diamine tetraacetate, vitamin C, glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol. Wherein the water phase comprises folium Rubi extract, tartaric acid, disodium edetate, and vitamin C, wherein folium Rubi extract 5%, tartaric acid 1%, disodium edetate 0.2%, and vitamin C1.5%. The oil phase comprises glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline, and cetyl alcohol, wherein glycerol accounts for 25%, white vaseline accounts for 30%, liquid paraffin accounts for 30%, salicylic acid accounts for 6%, vitamin E accounts for 3%, ethylparaben accounts for 3%, glyceryl monostearate accounts for 2%, and cetyl alcohol accounts for 1%.
The specific preparation method of the cream for treating the mastitis of the dairy cattle comprises the following steps:
drying the raspberry leaves, smashing the raspberry leaves into powder, weighing 20g of leaves, adding 4g of tartaric acid into 1000mL of 50% ethanol solution, performing ultrasonic assisted extraction for 40min at the temperature of 60-65 ℃ by using 250w of power, filtering insoluble substances after extraction is completed, and taking filtrate to obtain the extract of the raspberry leaves.
② the extract of the cotyledon of the raspberry is concentrated by rotary evaporation, the extract is extracted and decolored by equal volume of ethyl acetate after being concentrated to 60mL, and the extracted water phase is extracted by equal volume of n-butanol. And finally, carrying out rotary evaporation on the n-butanol phase at the temperature of 40-50 ℃ to obtain a solid substance, thus obtaining the raspberry leaf extract.
③ dissolving 0.5g of the extract of the raspberry leaf in 10mL of tartaric acid aqueous solution with the mass volume fraction of 1 percent, and adding 0.02g of disodium ethylene diamine tetraacetate and 0.15g of vitamin C after the extract is completely dissolved to obtain the treated extract of the raspberry leaf.
Mixing glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol according to the proportion of 25% of glycerol, 30% of white vaseline, 30% of liquid paraffin, 6% of salicylic acid, 3% of vitamin E, 3% of ethylparaben, 2% of glyceryl monostearate and 1% of hexadecanol to obtain the mixed emulsion.
Fifthly, mixing the treated extract of the raspberry leaves with the mixed emulsion according to the proportion of 1:4, and emulsifying at the temperature of 35-50 ℃ to obtain the cream for treating the mastitis of the dairy cows.
The using method comprises the following steps:
when the cream is used, the cream for treating the mastitis of the dairy cow is coated on the skin around the mammary gland of the dairy cow, the cream is coated for 2 times in 24 hours, the coating interval is 12 hours each time, the cream is continuously coated for 2-6 days, the mastitis of the dairy cow completely disappears, and through the test of 30 dairy cows which cannot be cured by taking antibiotics, the cream prepared by the invention can quickly cure the mastitis of the dairy cow which cannot be cured by the antibiotics, and through long-time tracking, the condition of drug resistance is not found, and the cream is remarkably better than the existing antibiotic products on dairy cows with the mastitis repeatedly.
Example 4
The embodiment provides a cream for treating mastitis of dairy cows, which mainly comprises raspberry leaf extract, tartaric acid, disodium ethylene diamine tetraacetate, vitamin C, glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol.
Wherein the water phase comprises folium Rubi extract, tartaric acid, disodium edetate, and vitamin C, wherein the folium Rubi extract is 10%, tartaric acid is 3%, disodium edetate is 0.5%, and vitamin C is 0.5%. The oil phase comprises glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and cetyl alcohol, wherein the glycerol accounts for 15%, the white vaseline accounts for 20%, the liquid paraffin accounts for 40%, the salicylic acid accounts for 8%, the vitamin E accounts for 5%, the ethylparaben accounts for 5%, the glyceryl monostearate accounts for 4% and the cetyl alcohol accounts for 3%.
The mixing ratio of the oil phase and the water phase is 6: 1.
The preparation method is the same as that of example 3, and only the addition amount of each component is different.
Example 5
The embodiment provides a cream for treating mastitis of dairy cows, which mainly comprises raspberry leaf extract, tartaric acid, disodium ethylene diamine tetraacetate, vitamin C, glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol.
Wherein the water phase comprises folium Rubi extract, tartaric acid, disodium edetate, and vitamin C, wherein the folium Rubi extract accounts for 1%, tartaric acid accounts for 2%, disodium edetate accounts for 0.1%, and vitamin C accounts for 0.1%. The oil phase comprises glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and cetyl alcohol, wherein the glycerol accounts for 30%, the white vaseline accounts for 25%, the liquid paraffin accounts for 35%, the salicylic acid accounts for 3%, the vitamin E accounts for 2%, the ethylparaben accounts for 2%, the glyceryl monostearate accounts for 1% and the cetyl alcohol accounts for 2%.
The mixing ratio of the oil phase and the water phase is 2: 1.

Claims (6)

1. A cream for treating mastitis of dairy cows is characterized in that: the cream for treating the mastitis of the dairy cows comprises a raspberry leaf extract, wherein the preparation method of the raspberry leaf extract comprises the steps of drying and crushing the raspberry leaves, mixing the crushed raspberry leaves with tartaric acid, adding an ethanol solution with the volume fraction of 50-75%, extracting for 10-120 min at the temperature of 60-65 ℃, filtering out insoluble substances after extraction, concentrating, extracting and decoloring with ethyl acetate, and extracting the extracted water phase with n-butyl alcohol. And finally, performing rotary evaporation on the n-butanol phase to obtain a solid substance, namely the raspberry leaf extract.
2. A cream for the treatment of bovine mastitis according to claim 1, wherein: the cream for treating the mastitis of the dairy cattle comprises components of raspberry leaf extract, ethylene diamine tetraacetic acid, vitamin C, glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol; wherein the water phase comprises raspberry leaf extract, tartaric acid, disodium ethylene diamine tetraacetate and vitamin C, wherein the raspberry leaf extract accounts for 1-10%, the tartaric acid accounts for 1-3%, the disodium ethylene diamine tetraacetate accounts for 0.1-0.5% and the vitamin C accounts for 0.1-0.5%; the oil phase comprises 15-30% of glycerol, 20-30% of white vaseline, 30-40% of liquid paraffin, 3-6% of salicylic acid, 2-5% of vitamin E, 2-5% of ethylparaben, 1-4% of glyceryl monostearate and 1-3% of hexadecanol; the mixing ratio of the oil phase to the water phase is 6: 1-2: 1.
3. A cream for the treatment of bovine mastitis according to claim 1, wherein: the mass volume ratio of the crushed raspberry leaves to the ethanol water solution is 40-60 g/L, and the mass volume ratio of the tartaric acid to the ethanol water solution is 2.667-4 g/L.
4. A cream for the treatment of bovine mastitis according to claim 1, wherein: the mass volume ratio of the raspberry leaf powder to the pure water is 166.67-300 g/L, and the mass volume ratio of the tartaric acid to the pure water is 6.667-20 g/L.
5. A method of making a cream for treating bovine mastitis according to claim 2, wherein the method of making the cream for treating bovine mastitis comprises:
drying the raspberry leaves, crushing the raspberry leaves into powder, adding 50-75% ethanol solution by volume fraction, performing ultrasonic-assisted extraction at the temperature of 60-65 ℃, filtering insoluble substances after extraction, concentrating, extracting and decoloring with ethyl acetate, and extracting the extracted water phase with n-butyl alcohol. Finally, performing rotary evaporation on the n-butanol phase to obtain a solid substance which is the raspberry leaf extract;
dissolving the extract of the raspberry leaves in aqueous solution of tartaric acid according to a formula, adding disodium ethylene diamine tetraacetate and vitamin C after the extract is completely dissolved, and obtaining treated extract of the raspberry leaves; wherein, the extract of the raspberry leaves accounts for 1 to 10 percent, the tartaric acid accounts for 1 to 3 percent, the disodium ethylene diamine tetraacetate accounts for 0.1 to 0.5 percent, and the vitamin C accounts for 0.1 to 0.5 percent;
mixing glycerol, salicylic acid, vitamin E, ethylparaben, glyceryl monostearate, white vaseline and hexadecanol to obtain a mixed emulsion; wherein, the glycerin accounts for 15-30%, the white vaseline accounts for 20-30%, the liquid paraffin accounts for 30-40%, the salicylic acid accounts for 3-6%, the vitamin E accounts for 2-5%, the ethylparaben accounts for 2-5%, the glyceryl monostearate accounts for 1-4%, and the cetyl alcohol accounts for 1-3%;
mixing the treated extract of the cotyledon of the raspberry with the mixed emulsion according to the proportion of 1:4, and emulsifying at the temperature of 35-50 ℃ to obtain the cream for treating the mastitis of the dairy cows.
6. Use of an extract of raspberry leaves, characterized in that: the raspberry leaf extract is used for inhibiting staphylococcus aureus, and is prepared by the steps of drying and crushing the raspberry leaves, mixing the crushed raspberry leaves with tartaric acid, adding 50-75% ethanol solution by volume fraction, extracting for 10-120 min at the temperature of 60-65 ℃, filtering out insoluble substances after extraction, concentrating, extracting and decoloring with ethyl acetate, and extracting the extracted water phase with n-butyl alcohol. And finally, performing rotary evaporation on the n-butanol phase to obtain a solid substance, namely the raspberry leaf extract. .
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