CN113940907A - Anti-dandruff composition containing synergistic antibacterial ingredients and application thereof - Google Patents
Anti-dandruff composition containing synergistic antibacterial ingredients and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
An anti-dandruff composition containing synergistic antibacterial components comprises the following components: purslane extract, clove flower extract, quinine and salts thereof; the anti-dandruff mixture comprises the following components: bacteriostatic components, auxiliary anti-dandruff components, polyhydric alcohol and deionized water; the preparation method of the mixture comprises the following steps: (1) dissolving polyalcohol, quinine and its salt, flos Caryophylli extract, and white willow bark extract under stirring; (2) adding herba Portulacae extract, Hamamelis virginiana extract and deionized water, stirring and dissolving to obtain mixture; the anti-dandruff mixture shampoo containing the synergistic antibacterial component comprises the following components: the composition comprises an anti-dandruff composition, sodium laureth sulfate, sodium lauroyl sarcosinate, decyl glucoside, cocamidopropyl betaine, a proper amount of citric acid, p-hydroxyacetophenone, 1, 2-hexanediol and deionized water. The invention utilizes the synergistic bacteriostasis among the components to achieve the effect of removing dandruff which is more than the simple addition of the components.
Description
Technical Field
The invention relates to the field of daily chemicals, in particular to an anti-dandruff composition containing a synergistic antibacterial component and application thereof.
Background
Dandruff is a common problem, and typical dandruff is flaky dandruff on or in the scalp and is mostly accompanied by marked itching. Various intrinsic and extrinsic environmental factors, such as sebum secretion, malassezia colonization, individual susceptibility, and the interaction between these factors, are responsible for the development of dandruff.
Malassezia is a lipotrophic fungus that parasitizes on the skin surfaces of humans and animals and secretes a variety of lipases with broad-spectrum activity that hydrolyze triglycerides in sebum to fatty acids. Malassezia has been considered to be a causative bacteria of dandruff, with malassezia restricta being the most prominent pathogenic bacterium, since it metabolizes saturated fatty acids and thereby produces an accumulation of proinflammatory unsaturated fatty acids, stimulating the skin barrier and causing the stratum corneum to shed.
The traditional dandruff removing agents for inhibiting malassezia are ketoconazole, ZPT, OCT and climbazole (climbazole), however, the chemical substances have certain side effects and are large in irritation, and scalp microorganisms are easy to generate drug resistance. For example, ZPT is easy to deposit on scalp, so that hair is withered and yellow and easy to break; therefore, many plant extract raw materials with anti-dandruff activity are emerging in the field of daily chemicals, but most of the plant extract raw materials have no ideal using effect.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide the plant extract with good anti-dandruff effect.
In order to solve the technical problems, the invention adopts the technical scheme that the anti-dandruff composition containing the synergistic antibacterial component comprises the following components in parts by mass:
1-5 parts of a purslane extract;
0.01-3 parts of a clove flower extract;
0.05-0.5 part of quinine and salts thereof.
The other technical scheme adopted by the invention is that the anti-dandruff mixture containing the synergistic antibacterial component comprises the following components in percentage by mass:
10-20% of antibacterial component;
2-10% of auxiliary anti-dandruff components;
20-40% of polyol;
the balance of deionized water.
The antibacterial component is the anti-dandruff composition containing the synergistic antibacterial component; the auxiliary anti-dandruff component is at least one of Hamamelis virginiana extract and white willow bark extract; the polyalcohol is at least one of propylene glycol and butanediol.
The invention also provides a preparation method of the anti-dandruff mixture containing the synergistic antibacterial component, which comprises the following steps:
(1) dissolving polyalcohol, quinine and its salt, flos Caryophylli extract, and white willow bark extract at 40-50 deg.C under stirring;
(2) adding herba Portulacae extract and Hamamelis virginiana extract, adding deionized water to 100%, stirring and dissolving to obtain mixture.
The invention adopts another technical scheme that the anti-dandruff composition shampoo containing the synergistic antibacterial component comprises the following components in percentage by mass:
1-20% of an anti-dandruff composition;
10-20% of sodium laureth sulfate;
1-4% of sodium lauroyl sarcosinate;
1-5% of decyl glucoside;
1-4% of cocamidopropyl betaine;
0.5-2% of sodium chloride;
proper amount of citric acid;
0.2-1% of p-hydroxyacetophenone;
0.2-1% of 1, 2-hexanediol;
the balance of deionized water.
The invention also provides a preparation method of the anti-dandruff composition shampoo containing the synergistic antibacterial component, which comprises the following steps:
(1) mixing sodium laureth sulfate, sodium lauroyl sarcosinate and decyl glucoside with deionized water, heating to 80-85 deg.C, stirring and dissolving;
(2) cooling to 60 deg.C, sequentially adding p-hydroxyacetophenone, 1, 2-hexanediol, and cocamidopropyl betaine, mixing and stirring;
(3) and continuously cooling to 45 ℃, adding a proper amount of citric acid and sodium chloride, uniformly stirring, sampling, detecting and discharging to obtain a finished product.
Compared with the prior art, the invention provides the plant extract consisting of the purslane extract, the clove flower extract, quinine and salts thereof, and achieves the anti-dandruff effect which is greater than the simply added anti-dandruff effect among the components by utilizing the synergistic antibacterial action among the components.
Detailed Description
The following examples and experiments are provided to further illustrate the embodiments of the present invention, but are not intended to limit the present invention.
An anti-dandruff composition containing synergistic antibacterial components comprises the following components in parts by mass
Table 1:
| components | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 |
| Purslane extract | 1 part of | 5 portions of | 3 portions of | 4 portions of | 1 part of |
| Clove flower extract | 0.01 part | 3 portions of | 2 portions of | 1 part of | 2.5 parts of |
| Quinine and its salts | 0.05 part | 0.5 portion | 0.1 part | 0.3 part | 0.25 part |
Purslane is an annual fleshy herbaceous plant of portulaca in the purslane family, is distributed in China except in alpine regions, has complex components, and mainly comprises alkaloid, coumarin compounds, flavonoid, phenol, terpenoid, sterol and other compounds. The purslane extract is prepared by taking overground whole-plant parts of purslane as raw materials, and is a natural cosmetic raw material with full water solubility. The purslane extract is a plant cosmetic raw material with various biological activities. The acne-removing cream is mainly used for resisting allergy, inflammation and inflammation, resisting external irritation to skin, has an acne-removing function, has an obvious relieving effect on skin allergy generated by long-term use of hormone cosmetics, can prevent and treat skin diseases such as skin eczema, allergic dermatitis, contact dermatitis and the like, and has whitening and anti-aging effects.
The flos Caryophylli extract is derived from flos Caryophylli of Myrtaceae, and mainly contains eugenol, acetyl eugenol, caryophyllene, vanillin and ester, and has antibacterial and antiinflammatory effects, and is suitable for various skin and scalp with high safety.
Quinine, also known as cinchona alkaloid, is a main alkaloid in the bark of madder plant cinchona, has the effects of inhibiting the reproduction of or killing plasmodium falciparum erythrocytic endophytic protozoa, is an important antimalarial drug, also has the anti-inflammatory effect, and can be used for treating rheumatoid arthritis.
In order to verify the synergistic antibacterial effect of the anti-dandruff composition containing the synergistic antibacterial component, the following experiments are set, and the steps are as follows:
(1) preparing a restriction malassezia suspension;
(2) leeming & Notman medium was incubated at 50 ℃ for future use. Marking 16 sterile culture dishes on a dish cover by using a marker pen, wherein 2 sterile culture dishes are A, B, C, A + B, A + C, B + C, A + B + C, and 2 negative control dishes are adopted;
(3)1 sterile test tube is taken, numbered 10-2 in sequence, and 4.5mL of sterile normal saline is added into the test tube. 0.5mL of the bacterial suspension was taken with 1-mL sterile pipette, added to a 10-1 tube, and shaken well. Another 1-1 mL sterile pipette is taken to suck 0.5mL deionized water sample from the 10-1 test tube to the 10-2 test tube and is mixed uniformly for standby;
(4) 1mL of the diluted sample in a 10-2 test tube was added to each sterile petri dish labeled A, B, C, A + B, A + C, B + C, A + B + C and negative controls, and 15g of Leeming & Notman medium incubated at 50 ℃ was immediately added, shaken up quickly and gently to avoid shaking the medium out of the petri dish. Wherein the dish labeled A is added with Leeming & Notman medium mixed with 0.075g of quinine and its salts, similarly, B is added with 0.75g of clove extract, C is added with 0.3g of purslane extract, A + B is a mixture of 0.075g of quinine and its salts and 0.75g of clove extract, A + C is a mixture of 0.075g of quinine and its salts and 0.3g of purslane extract, B + C is a mixture of 0.75g of clove extract and 0.3g of purslane extract, and A + B + C is a mixture of 0.075g of quinine and its salts, 0.75g of clove extract and 0.3g of purslane extract. The Leeming & Notman culture medium without the composition components is used as a negative control, and after the flat plate is well mixed, the flat plate is immediately laid on the table top until the flat plate is completely solidified;
(5) the completely solidified measuring plate is inverted and cultured for 72h at 28 ℃;
(6) taking out the plates, observing and counting the colony number of each plate, and calculating the bacteriostasis rate, wherein the formula for calculating the bacteriostasis rate is as follows: the bacteriostatic rate (%) is (number of colonies in negative control test-number of colonies in each group)/number of colonies in negative control test × 100%;
(7) the data obtained are reported in table 2 below:
| item | A | B | C | A+B | A+C | B+C | A+B+C |
| Bacteriostatic ratio (%) | 63.8 | 45.6 | 38.1 | 85.2 | 76.7 | 68.4 | 96.1 |
| ω | 0.362 | 0.544 | 0.619 | 0.148 | 0.233 | 0.316 | 0.039 |
The verification of the synergistic effect of the anti-dandruff composition containing the synergistic antibacterial component uses the formula and the principle described in the Enhanced identification of synthetic and antibacterial ingredients and the atmosphere or more drugs (DOI:10.1098/rsif.2016.0332), and concretely comprises the following steps:
in the formula:
e represents a measure of the interaction of the composition, the interaction being synergistic when E < -0.5 and antagonistic when E >0.5, a simple addition when E >0.5 < 0.5.
ω D represents the growth rate of malassezia restricta in the dishes of each group relative to the negative control after addition of D, where D ═ (a, B, C, AB, AC, BC, ABC), i.e. single material or mixture of several materials. That is, ω is the colony count of each group of petri dishes/the colony count of the negative control is 1-bacteriostasis rate.
min () represents the group with the smallest growth rate in parentheses, and the calculation formula based on the value of E yields E-0.85 < -0.5, indicating that the combination of three components, quinine and its salts (A), clove extract (B), and purslane extract (C), has synergistic inhibitory effect on Maraxella restricta.
An anti-dandruff mixture containing synergistic bacteriostatic components comprises the following components in percentage by mass as shown in examples 6 to 9 of the following table 3:
the Hamamelis virginiana extract is prepared from dried Hamamelis virginiana bark, is a natural cosmetic material with full water solubility, and is easily soluble in ethanol and glycerol. It can relieve irritated and allergic skin, fully astringe follicular orifice, prevent blackhead and acne, and has antiinflammatory, antiallergic, and astringent effects. Meanwhile, the skin care product has deep cleaning and anti-aging skin care effects and has long and wide application in the cosmetic market.
The white willow bark extract is extracted from dried bark of white willow of Salix of Salicaceae, and contains salicin and dextran as main active ingredients. Salicin has aspirin-like properties, is an effective anti-inflammatory ingredient, and has been traditionally used to heal wounds and relieve muscle pain. The glucan has the effects of improving the immunity of the organism, activating the cell activity and achieving the anti-inflammation and anti-wrinkle effects. Studies find that salicin is an oxidase inhibitor and has the effects of resisting wrinkles, increasing skin glossiness and elasticity, reducing pigmentation, increasing skin moisture and the like. The white willow bark extract has the skin care effects of resisting aging, removing cutin and removing acne in cosmetics.
The method for preparing the anti-dandruff mixture containing the synergistic bacteriostatic component in the embodiments 6 to 9 comprises the following steps:
(1) dissolving polyalcohol, quinine and its salt, flos Caryophylli extract, and white willow bark extract at 40-50 deg.C under stirring;
(2) adding herba Portulacae extract and Hamamelis virginiana extract, adding deionized water to 100%, stirring and dissolving to obtain mixture.
Example 10:
an anti-dandruff composition shampoo containing a synergistic antibacterial component comprises the following components in percentage by mass:
20% of an anti-dandruff composition;
10% of sodium laureth sulfate;
4% of sodium lauroyl sarcosinate;
decyl glucoside 1%;
cocamidopropyl betaine 4%;
0.5 percent of sodium chloride;
proper amount of citric acid;
0.2 percent of p-hydroxyacetophenone;
1% of 1, 2-hexanediol;
the balance of deionized water.
Example 11:
an anti-dandruff composition shampoo containing a synergistic antibacterial component comprises the following components in percentage by mass:
10% of an anti-dandruff composition;
15% of sodium laureth sulfate;
2% of sodium lauroyl sarcosinate;
decyl glucoside 3%;
2% of cocamidopropyl betaine;
1.2 percent of sodium chloride;
proper amount of citric acid;
0.5 percent of p-hydroxyacetophenone;
0.5 percent of 1, 2-hexanediol;
the balance of deionized water.
Example 12:
an anti-dandruff composition shampoo containing a synergistic antibacterial component comprises the following components in percentage by mass:
1% of an anti-dandruff composition;
20% of sodium laureth sulfate;
1% of sodium lauroyl sarcosinate;
decyl glucoside 5%;
1% of cocamidopropyl betaine;
2% of sodium chloride;
proper amount of citric acid;
1% of p-hydroxyacetophenone;
0.2% of 1, 2-hexanediol;
the balance of deionized water.
The method for preparing the anti-dandruff composition shampoo containing the synergistic bacteriostatic components of the above examples 10 to 12 comprises the following steps:
(1) mixing sodium laureth sulfate, sodium lauroyl sarcosinate and decyl glucoside with deionized water, heating to 80-85 deg.C, stirring and dissolving;
(2) cooling to 60 deg.C, sequentially adding p-hydroxyacetophenone, 1, 2-hexanediol, and cocamidopropyl betaine, mixing and stirring;
(3) and continuously cooling to 45 ℃, adding a proper amount of citric acid and sodium chloride, uniformly stirring, sampling, detecting and discharging to obtain a finished product.
In order to verify the efficacy of the anti-dandruff composition shampoo containing the synergistic antibacterial component, comparative experiments of examples and comparative examples were carried out:
volunteers were recruited to use the samples of examples 13-16 and comparative examples 5-8 described above.
And (3) inclusion standard: has obvious dandruff and exuberant scalp oil secretion.
Discharge criteria:
(1) scalp skin diseases such as eczema, psoriasis, tinea capitis, asbestos-like pityriasis or obvious seborrheic dermatitis on the head;
(2) participating in clinical drug test or other testers within 30 days;
(3) the patients with external or internal antifungal drugs or glucocorticoids within 1 month.
Basic conditions are as follows: a total of 80 subjects, 46 males and 34 females, aged between 20-51 years, were enrolled in 8 groups and 10 individuals/group using the samples from examples 13-16 and comparative examples 5-8, respectively.
The condition of dandruff and scalp oil was classified into 5 grades (observed after 48 hours without washing hair), 0: substantially free of; 1: slightly; 2: moderate; 3: severe; 4: it is very serious. Observations were recorded before and 4 weeks after the test product application, respectively, and the results are shown in the following table:
it is understood from example 13 and comparative example 5 that the bacteriostatic component is important for the anti-dandruff effect, and it is understood from example 13 and comparative example 8 that the anti-dandruff assisting component has a great synergistic effect on the anti-dandruff effect.
Compared with the prior art, the invention provides the plant extract consisting of the purslane extract, the clove flower extract, quinine and salts thereof, and achieves the anti-dandruff effect which is greater than the simply added anti-dandruff effect among the components by utilizing the synergistic antibacterial action among the components.
The embodiments of the present invention have been described in detail with reference to the examples, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention.
Claims (5)
1. An anti-dandruff composition containing a synergistic antibacterial component is characterized by comprising the following components in parts by mass:
1-5 parts of a purslane extract;
0.01-3 parts of a clove flower extract;
0.05-0.5 part of quinine and salts thereof.
2. An anti-dandruff mixture containing a synergistic antibacterial component is characterized by comprising the following components in percentage by mass:
10-20% of antibacterial component;
2-10% of auxiliary anti-dandruff components;
20-40% of polyol;
the balance of deionized water;
the bacteriostatic component is the anti-dandruff composition containing the synergistic bacteriostatic component of claim 1; the auxiliary anti-dandruff component is at least one of Hamamelis virginiana extract and white willow bark extract; the polyalcohol is at least one of propylene glycol and butanediol.
3. A method of preparing an antidandruff mixture containing synergistic bacteriostatic components according to claim 2, comprising the steps of:
(1) dissolving polyalcohol, quinine and its salt, flos Caryophylli extract, and white willow bark extract at 40-50 deg.C under stirring;
(2) adding herba Portulacae extract and Hamamelis virginiana extract, adding deionized water to 100%, stirring and dissolving to obtain mixture.
4. An anti-dandruff mixture shampoo containing the synergistic antibacterial component as claimed in claim 2, which comprises the following components in percentage by mass:
1-20% of an anti-dandruff composition;
10-20% of sodium laureth sulfate;
1-4% of sodium lauroyl sarcosinate;
1-5% of decyl glucoside;
1-4% of cocamidopropyl betaine;
0.5-2% of sodium chloride;
proper amount of citric acid;
0.2-1% of p-hydroxyacetophenone;
0.2-1% of 1, 2-hexanediol;
the balance of deionized water.
5. The preparation method of the anti-dandruff mixture shampoo containing the synergistic bacteriostatic component as claimed in claim 4, comprising the following steps:
(1) mixing sodium laureth sulfate, sodium lauroyl sarcosinate and decyl glucoside with deionized water, heating to 80-85 deg.C, stirring and dissolving;
(2) cooling to 60 deg.C, sequentially adding p-hydroxyacetophenone, 1, 2-hexanediol, and cocamidopropyl betaine, mixing and stirring;
(3) and continuously cooling to 45 ℃, adding a proper amount of citric acid and sodium chloride, uniformly stirring, sampling, detecting and discharging to obtain a finished product.
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| CN115671014A (en) * | 2022-10-24 | 2023-02-03 | 广州市圣莎拉化妆品有限公司 | Plant dandruff-removing and itching-relieving composition and shampoo |
| CN115813841A (en) * | 2023-01-10 | 2023-03-21 | 广东迪美新材料科技有限公司 | Anti-dandruff and itching-relieving composition, preparation method and application thereof, shampoo and preparation method |
| CN115813841B (en) * | 2023-01-10 | 2023-09-05 | 广东迪美新材料科技有限公司 | Anti-dandruff itching-relieving composition, preparation method and application thereof, shampoo and preparation method |
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