CN104083473A - Application of raspberry extract in preparation of drug for treating perimenopausal syndrome - Google Patents
Application of raspberry extract in preparation of drug for treating perimenopausal syndrome Download PDFInfo
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Abstract
The invention relates to an application of raspberry extract in preparation of a drug for treating perimenopausal syndrome. The raspberry extract is used for effectively solving the drug problem of treating the perimenopausal syndrome. A preparation method of the raspberry extract comprises the following steps: crushing raspberry into coarse powder, carrying out reflux extraction and degreasing by using petroleum ether and discarding the petroleum ether; volatilizing the petroleum ether in the raspberry residue, soaking by using ethyl alcohol, carrying out reflux extraction, filtering, recovering the ethyl alcohol from the filtrate under reduced pressure, adding distilled water to disperse, carrying out adsorption on a dispersion liquid by using macroporous adsorption resin, eluting by using distilled water firstly and discarding the water eluent; eluting by using ethyl alcohol and discarding the eluent; eluting by using ethyl alcohol again, recovering the ethyl alcohol from the ethyl alcohol eluent under reduced pressure till no alcohol smell, carrying out freeze drying and crushing. The raspberry extract has the effects of remarkably increasing thymus, spleen and uterus indexes and is effectively used for preparing the drug for treating the perimenopausal syndrome; and the application of the raspberry extract in preparation of the drug for treating the perimenopausal syndrome is realized.
Description
Technical field
The present invention relates to medicine, the particularly application of a kind of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine.
Background technology
Perimenopausal syndrome claims again climacteric syndrome (MPS), refer to occur gonadal hormone fluctuation before and after postmenopausal women or reduce due to a series ofly take function disorder of autonomic nervous system as main, with one group of syndrome of neuropsychological symptom.Menopause can be divided into two kinds of natural menopause and artificial menopauses.Natural menopause refers in ovary that follicle is used up, or remaining follicle lost reaction to promoting sexual gland hormone, and estrogen is no longer grown and secrete to follicle, can not grow by stimulon Endometrium, causes menopause.
Fructus Rubi (formal name used at school: Rubus idaeus) having another name called Fructus Rubi, tree Wo Pao, Fructus Rubi, Mai Pao (harvesting wheat is result during season, therefore named) etc., is the xylophyta of a kind of Rosaceae rubus, belong to fruits, fruit taste is sweet, and plant is shrub, high 1-2 rice; Branch brown or bronzing, by fine hair shape pubescence, dredge rawhide thorn, a little as Fructus Fragariae Ananssae during children.
The fruit of Fructus Rubi is a kind of aggregate fruit, there is redness, gold and black, in China, distribute in a large number but little known, there is a small amount of cultivation in area northeastward, and also there is distribution in forest land, northeast, mountain region, one band mountain forest also has a large amount of distributions in Jiangsu and Zhejiang Provinces, belong to wild fruit, few people cultivate and peddle, and the mature period has market among a small circle northeastward.Fructus Rubi plant can be used as medicine, and has multi-medicament to be worth, and its fruit has the effect of kidney invigorating and YANG supporting.Raspberry seed oil belongs to unsaturated fatty acid, can promote prostate secreting hormone.Though the existing many-side research relevant for Fructus Rubi, the open report that so far there are no applies in preparation treatment perimenopausal syndrome medicine relevant for Fructus Rubi extract.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the present invention's object is just to provide the application of a kind of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, can effectively solve the medication problem for the treatment of perimenopausal syndrome.
The technical scheme that the present invention solves is, the application of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, and this Fructus Rubi extract is, Fructus Rubi is ground into coarse powder, with the defat of petroleum ether reflux, extract,, discards petroleum ether; Fructus Rubi medicinal residues volatilize petroleum ether, by soak with ethanol, reflux, extract,, filter, decompression filtrate recycling ethanol is extremely without alcohol taste, and adding distil water is dispersed to and is equivalent to, containing crude drug amount 0.3g/ml, obtain dispersion liquid, on dispersion liquid, AB-8 type macroporous adsorbent resin, first uses distilled water eluting, discards water liquid; The ethanol elution that to continue by volumetric concentration be 10%, discards eluent; The ethanol elution that is 80% by volumetric concentration again, collected volume concentration 80% ethanol elution, decompression recycling ethanol is extremely without alcohol taste, and lyophilization, pulverizes, and obtains Fructus Rubi extract, and in Fructus Rubi extract, Fructus Rubi total flavones mass content is more than 57%.
Preparation method of the present invention is simple, abundant raw materials, prepared Fructus Rubi extract has the effect that significantly improves thymus, spleen, uterus index, change the state of endometrium, body of gland, flesh layer, can be effective to preparation treatment perimenopausal syndrome medicine, realize the application of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, opened up medical value and the new purposes of Fructus Rubi.
The specific embodiment
Below in conjunction with concrete condition, the specific embodiment of the present invention is elaborated.
The present invention is in concrete enforcement, and Fructus Rubi extract is treated the application in perimenopausal syndrome medicine in preparation, and this Fructus Rubi extract is, it was the coarse powder of 20-30 mesh sieve that Fructus Rubi is pulverized, the petroleum ether reflux, extract, 1h that first adds 10 times of Fructus Rubi weight, defat, discards petroleum ether; Fructus Rubi medicinal residues volatilize petroleum ether, the soak with ethanol 0.5h that the volumetric concentration that adds 10 times of Fructus Rubi weight is 80%, reflux, extract, 2 times, each 1h, filter, merging filtrate, decompression recycling ethanol is extremely without alcohol taste, and adding distil water is dispersed to and is equivalent to containing crude drug amount 0.3g/ml, obtain dispersion liquid, AB-8 type macroporous adsorbent resin on dispersion liquid, first uses 2 times of column volume distilled water eluting, discards water liquid; The ethanol elution that the volumetric concentration continuing with 4 times of column volumes is 10%, discards eluent; The ethanol elution that is 80% by the volumetric concentration of 6 times of column volumes again, collected volume concentration 80% ethanol elution, decompression recycling ethanol is to without alcohol taste, lyophilization, pulverize, obtain Fructus Rubi extract, in Fructus Rubi extract, Fructus Rubi total flavones mass content is more than 57%.
Fructus Rubi extract of the present invention has the effect that significantly improves thymus, spleen, uterus index, change the state of endometrium, body of gland, flesh layer, can be effective to preparation treatment perimenopausal syndrome medicine, realize the application of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, and having obtained valid certificates through test, related tests data is as follows.
The impact of Fructus Rubi extract on mice climacteric model
1 experiment material
1.1 laboratory animal
SPF level mice, Kunming kind, female, 20-25g, is provided by Wuhan Biological Products Inst..Quality certification numbering: 42000400000611; Laboratory quality certification SYXK (Henan) 2010-001.
1.2 Experimental agents
Fructus Rubi extract of the present invention; Geng Nian An Capsules, commercially available; Soybean isoflavone dimension E soft capsule, commercially available.
1.3 experiment reagent
Sodium carboxymethyl cellulose, Tianjin star chemistry reagent Manufacturing Co., Ltd; Benzylpenicillin sodium for injection, North China pharmaceutical Co. Ltd; Formalin (analytical pure), Yantai City is Chemical Co., Ltd. in pairs; 0.9% sodium chloride injection, Cisen Pharmaceutical Co., Ltd.; Chloral hydrate, Tianjin recovery fine chemistry industry institute; Mice E2 ELISA detection kit, R & D company; Mice T ELISA detection kit, R & D company; Mice LH ELISA detection kit, R & D company; Mice FSH ELISA detection kit, R & D company.
1.4 experimental apparatus
Electronic scale, Shanghai Minqiao Medical Apparatus and Instruments Co., Ltd., model JY601; Electronic analytical balance, Ao Haosi (Shanghai) company, model AR1140/C; High speed tabletop centrifuge, Anting Scientific Instrument Factory, Shanghai, model TGL-168; Electric-heated thermostatic water bath, Shanghai Yiheng Scientific Instruments Co., Ltd, model HWS12; Mice autonomic activities tester, Chengdu TME Technology Co., Ltd., model ZZ-6; Mice is kept away dark instrument, Chengdu TME Technology Co., Ltd., model BA-200; High speed refrigerated centrifuge, Zhong Jia branch company of Keda Innovation Co., Ltd, model KDC-160HR; Adjustable pipette, Shanghai Lei Bo Analytical Instrument Co., Ltd; Microplate reader, U.S. BIO-RAD company, model 680; Electric microscope, Japanese OLYMPUS company, model BX61.
2 experimental techniques
2.1 modelings and administration
Modeling method: get 100 of the female kunming mices of body weight 23～25g, get at random outside blank group of 12 conducts, the surgical procedure that rehearses, all the other mices are made climacteric model.Mice weigh pneumoretroperitoneum inject 10% chloral hydrate (0.03ml/10g) anesthesia after abdomen position fix, then from mouse back most end rib, at midaxillary line with apart from about 1cm infall cropping outside spinal column, after sterilization, cut approximately 0.5～1cm of skin and dorsal muscles, the shinny liparitosis of a visible milky in the otch visual field, ovary be embedding wherein.With pincet, clamp gently liparitosis and pull out outside otch, fractionation of fatty group, can see a thin-line-shaped irregular ovary that is yellowish red color.During clip, first fallopian tube under ovary (comprising fat) is used to fine rule ligation, then extract ovary, postoperative taking advantage of a situation put back to cornua uteri in abdominal cavity, and suture muscles and skin are extractd bilateral ovaries with method.Postoperative meticulous raising, intramuscular injection penicillin 200,000 u/kg (every 0.1mL) are in case infect continuous 3d, every day 1 time.After operation 5d, start by only carrying out mouse vagina plate coating checking, every day 1 time, whether completely continuous 5d, determine ovary excision.The mice that smear presents emotionally reaction is discarded, selects 72 castrations completely mice be evenly divided at random 6 groups for testing, be respectively model group, Geng Nian An Capsules group, soybean isoflavone soft capsule group, large, medium and small dosage Fructus Rubi extract group.
Dosage: 0.5%CMC compound method: take sodium carboxymethyl cellulose 4g, be made into 800ml with distilled water.Large, medium and small dosage Fructus Rubi extract group dosage is respectively 1500mg/kg, 750mg/kg, 375mg/kg (administration volume 0.1ml/10g).Collocation method: take respectively Fructus Rubi extract 15,7.5g, 3.75g, first with a small amount of 0.5%CMC, dissolve, and then standardize solution is to 100ml, mixes.Geng Nian An Capsules (675mg/kg, is made into 67.5mg/ml with distilled water, and 0.1ml/10g is equivalent to 15 times of quantity); Collocation method: get 9 of Geng Nian An Capsules, first dissolve with a small amount of 0.5%CMC, and then standardize solution is to 40ml, mixes, and is the dosage (675mg/kg) of Geng Nian An Capsules suspension.Soybean isoflavone dimension E soft capsule (250mg/kg, is made into 25mg/ml with distilled water, and 0.1ml/10g is equivalent to 15 times of quantity); Collocation method: get 2 of soybean isoflavone capsules, first dissolve with a small amount of 0.5%CMC, and then standardize solution is to 40ml, mixes, and is the dosage (250mg/kg) of soybean isoflavone capsule suspension.
Storage method: cold preservation, used time water proof is warm.
Medication: each treated animal gives relative medicine on the 10th day after operation.Geng Nian An Capsules group gavages Geng Nian An Capsules suspension 675mgkg
-1, soybean isoflavone soft capsule group gavages soybean isoflavone soft capsule suspension 250mgkg
-1, large, medium and small dosage Fructus Rubi extract group gavages large, medium and small dosage Fructus Rubi extract 200mgkg by group respectively
-1, 10mgkg
-1, 50mgkg
-1, 0.1ml/10g.Blank group and model group gavage respectively same volume 0.5%CMC solution, and every day, gastric infusion was 1 time, successive administration 21d.
2.2 observation items and detection method
Each is organized mice and in administration, in the time of 18 days, measures autonomic activities number of times in 5min, measures mice and enter first in incubation period in darkroom and 5min and enter the number of times being shocked by electricity in darkroom in the time of administration 19-20 days.2h after last administration (water 12h is can't help in fasting), mice is plucked eyeball after weighing and gets blood, separation of serum, the content of E2, T, LH, FSH in mensuration serum; Then de-cervical vertebra is put to death mice, dissects mice, extracts thymus, spleen, uterine cancer cell, weighs its weight in wet base and calculates organ index (organ index=internal organs weight in wet base mg/ Mouse Weight g), then gets hypophysis; Thymus, spleen, uterus, hypophysis are fixed in 10% formalin solution, paraffin embedding, section, HE dyeing, the tectology of each group of light Microscopic observation changes.
2.2.1 autonomic activities number of times test
Each group mice is put into autonomic activities instrument, and the 1min that first conforms, then measures autonomic activities number of times in 5min.
2.2.2 darkness avoidance test test
Each group mouse tail is put into and measured box facing to the osculum that enters darkroom, train, after 24h, redeterminate mice and enter first in incubation period in darkroom and 5min and enter the number of times being shocked by electricity in darkroom.
2.2.3 kit measurement method
Obtaining of serum specimen: use containing pyrogen and endotoxic test tube, do not avoid any cytositimulation in operating process, collect after blood, 3000 to leave the heart 10 minutes careful separated rapidly by serum and erythrocyte.
Mouse E2 (E
2) ELISA detection kit assay method: from the aluminium foil bag equilibrium at room temperature 20min, take out required lath, residue lath is used from the sealing of seal bag and is put back to 4 ℃.Standard substance hole and sample aperture are set, and standard substance hole respectively adds the standard substance 50 μ L of variable concentrations.In sample aperture, first add sample to be tested 10 μ L, then add sample diluent 40 μ L (being 5 times of sample dilutions); Blank well does not add.Except blank well, in standard substance hole and sample aperture, every hole adds the detection antibody 100 μ L of horseradish peroxidase-labeled, with shrouding film, seals reacting hole.37 ℃ of water-baths or calorstat incubation 60min.Discard liquid, in absorbent paper, pat dry, cleaning mixture is filled it up with in every hole, standing 1min.Get rid of cleaning mixture, in absorbent paper, pat dry, so repeat to wash plate 5 times.Every hole first adds substrate A, each 50 μ L of B, and 37 ℃ of lucifuges are hatched 15min.Take out ELISA Plate, every hole adds stop buffer 50 μ L, in 15min, and the light absorption value (OD value) in each hole of 450nm wavelength measurement.According to the concentration of standard substance and corresponding OD value, draw out standard substance regression curve, by curvilinear equation, calculate each sample concentration value.Ultimate density is that practical measurement concentration is multiplied by extension rate.0,4,8,16,32,64pmol/L wherein, standard substance (S0-S5) concentration is followed successively by:.
0,50,100,200,400,800pg/mL mice (Mouse) testosterone (T) ELISA detection kit standard substance (S0-55) concentration is followed successively by:;
0,0.5,1,2,4,8mIU/mL mice (Mouse) lutropin (LH) ELISA detection kit standard substance (S0-55) concentration is followed successively by:;
0,5,10,20,40,80mIU/mL mice (Mouse) follicle stimulating hormone (FSH) ELISA detection kit standard substance (S0-55) concentration is followed successively by:.
2.3 statistical procedures methods
Data analysis carries out the statistical procedures of data information, average ± standard deviation for measurement data with the medical statistics bag of SPSS17.0
represent, between each group, relatively adopt one factor analysis of variance, the neat person of variance test uses LSD method, and heterogeneity of variance person adopts Ridit check with Games-Howell method check, ranked data.
3. result
3.1 impacts on mice climacteric model autonomic activities
Mice autonomic activities test result is respectively organized in experiment, in Table 1.
The impact of table 1 Fructus Rubi extract on climacteric model mice autonomic activities
Note: with model group comparison, * P<0.05, * * P<0.01
As seen from the above table, with blank group ratio, the movable number of times of model group mice, the number of times of standing all significantly reduce (P<0.01), illustrate that climacteric model mice reduces the curious degree of fresh environment.With model group ratio, soybean isoflavone soft capsule, Geng Nian An Capsules, big or middle dosage Fructus Rubi extract group all can significantly improve the movable number of times of mice and the number of times of standing (P<0.01), and low dose of Fructus Rubi extract group can obviously improve the movable number of times of mice and the number of times (P<0.05) of standing.
3.2 keep away the dark impact of testing incubation period and being electrically shocked number of times to mice climacteric model
Experiment is respectively organized mice and is kept away dark experimental results, in Table 2.
Table 2 Fructus Rubi extract is kept away the impact of dark experiment incubation period and number of shocks on mice climacteric model
Note: with model group comparison, * P<0.05, * * P<0.01
As seen from Table 2, with blank group ratio, the incubation period of model group mice, number of shocks all significantly reduce (P<0.01), have reflected mice climacteric model hypomnesis.With model group ratio, Geng Nian An Capsules, soybean isoflavone soft capsule, big or middle dosage Fructus Rubi extract all can significantly improve the incubation period of keeping away dark experiment of mice, reduce number of shocks (P<0.01), improve mouse memory power; Low dose of Fructus Rubi extract can obviously reduce number of shocks (P<0.05).
3.3 impacts on mice climacteric model organ index
Mouse thymus, spleen, uterus index are respectively organized in experiment, in Table 3.
The impact of table 3 Fructus Rubi extract on mice climacteric model thymus, spleen, uterus index
Note: with model group comparison, * P<0.05, * * P<0.01
As can be seen from Table 3, with blank group ratio, the uterus index of model group mice significantly reduces (P<0.01), illustrates that extracing ovary causes climacteric model mice metratrophia, thereby extract ovary estrogen secretion deficiency, causes the atrophy of climacteric mouse immune organ.With model group ratio, soybean isoflavone soft capsule, Geng Nian An Capsules, big or middle dosage Fructus Rubi extract all can significantly improve thymus, spleen, uterus index (P<0.01).
3.4 impacts on mice climacteric model serum sex hormone level
Each organizes E2 in mice serum, T, FSH, LH content.In Table 4-5.
The impact of table 4 Fructus Rubi extract on E2, T level in mice climacteric model serum
Note: with model group comparison, * P<0.05, * * P<0.01
The impact of table 5 lock capsule on FSH, LH level in mice climacteric model serum
Note: with model group comparison, * P<0.05, * * P<0.01
From table 4～5, can find out, with blank group ratio, E2, T level significantly decline (P<0.01) in model group mice serum, LH, FSH level be significantly rising (P<0.01) all, illustrate that extracing ovary causes the sex hormone level in climacteric model mice serum disorderly, climacteric mouse model copies successfully.Dosage Fructus Rubi extract big or middle and Geng Nian An Capsules, soybean isoflavone soft capsule can significantly raise E2 in mice climacteric model serum, T level (P<0.01) and reduce FSH, the LH level raising in serum.Low dose of Fructus Rubi extract can obviously reduce FSH level and the T level (P<0.05) in serum.
3.5 impacts on climacteric model mice organ-tissue form
The histopathology observed result that Mouse Uterus, thymus, spleen, hypophysis are respectively organized in experiment is as follows, and climacteric type mice pathology photo in appendix 1 is shown in by pathology photo.
4.1 impacts on climacteric model mice uterus histomorphology
According to experiment, respectively organize the change in various degree of endometrium of mouse, body of gland, the appearance of flesh layer, adopt semi-quantitative standards, Pathomorphologic is divided into four ranks, experiment is respectively organized to Mouse Uterus and measure, observed result is in Table 7.
The affect unit of table 7 Fructus Rubi extract on the uterus pathological change of climacteric model mice: only
"-" endometrial epithelial cell, body of gland, flesh layer, serous coat are all normal; "+" endometrial epithelial cell, the atrophy of body of gland small part, flesh layer serous coat is all normal; " ++ " endometrial epithelial cell, the atrophy of body of gland part, the slightly atrophy of flesh layer, serous coat is normal; " +++ " endometrial epithelial cell, the obvious atrophy of body of gland, serous coat is normal.
Through Ridit check, as can be known from the above table, with blank group ratio, there is significant pathological tissue pathological changes (P<0.01) in model group Mouse Uterus.With model group ratio, soybean isoflavone dimension E soft capsule, Geng Nian An Capsules, middle dosage Fructus Rubi extract all can improve the uterus pathology lesion tissue (P<0.01) of mice significantly, and heavy dose of Fructus Rubi extract can significantly improve the uterus pathology lesion tissue (P<0.05) of mice.
4.2 impacts on mice climacteric model thymus, spleen tissue form
Adopt micrometer, experiment is respectively organized to every Mouse Thymic Cortex thickness and the narrowest place and measure its thickness and try to achieve mean; Adopt the baseline of micrometer to drop on splenic nodule, centered by central artery, measure respectively the splenic nodule thickness size of both sides, ask mean; The results are shown in Table 7.
The impact of table 7 lock capsule on climacteric model mice thymus, spleen pathological change
Note: with model group comparison, * P<0.05, * * P<0.01
From upper table, can find out, with blank group ratio, model group Mouse Thymic Cortex thickness, splenic nodule volume significantly reduce (P<0.01), illustrate that mice makes thymus after climacteric model, the atrophy of spleen volume.With model group ratio, heavy dose of Fructus Rubi extract can significantly thicken thymic cortex thickness and splenic nodule volume (P<0.01), and middle dosage Fructus Rubi extract can obviously thicken thymic cortex thickness (P<0.05).
4.3 impacts on mice climacteric model pituitary tissue form
Under high power field, dropping on area is basophil quantity and gene expression quantity in 10000 μ m2 micrometer rectangle frames.The results are shown in Table 8.
The impact of table 8 Fructus Rubi extract on the pathological change of climacteric model mice hypophysis
Note: with model group comparison, * P<0.05, * * P<0.01
As can be seen from the above table, with blank group ratio, model group basophil quantity and gene expression quantity significantly reduce (P<0.01), illustrate that mice makes after climacteric model, and the cell source of secretion promoting sexual gland hormone reduces.With model group ratio, big or middle dosage Fructus Rubi extract group can significantly increase basophilic cell quantity and gene expression quantity (P<0.01).
Above-mentioned testing data fully shows that Fructus Rubi extract of the present invention has the thymus of significantly improving, spleen, the effect of uterus index, be effective to preparation treatment perimenopausal syndrome medicine, realize the application of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, and opened up the new way of perimenopausal syndrome medicine for treatment, and through clinical, the 55 routine Patients with Perimenopausal Syndromes way of taking medicine is routinely taken, every day 3 times, each 1.5-2.0g, serve on 20-30 days, perimenopausal syndrome all has improvement in various degree, show no matter be through animal experiment, or through clinical verification, all prove that Fructus Rubi extract has the effect for the treatment of perimenopausal syndrome, realize the application of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, new purposes and the medical value of Fructus Rubi have been opened up, there is great practical value, that one on Chinese medicine is innovated greatly.
Claims (1)
1. the application of Fructus Rubi extract in preparation treatment perimenopausal syndrome medicine, this Fructus Rubi extract is, by Fructus Rubi pulverizing, is the coarse powder of 20-30 mesh sieve, first adds the petroleum ether reflux, extract, 1h of 10 times of Fructus Rubi weight, defat, discards petroleum ether; Fructus Rubi medicinal residues volatilize petroleum ether, the soak with ethanol 0.5h that the volumetric concentration that adds 10 times of Fructus Rubi weight is 80%, reflux, extract, 2 times, each 1h, filter, merging filtrate, decompression recycling ethanol is extremely without alcohol taste, and adding distil water is dispersed to and is equivalent to containing crude drug amount 0.3g/ml, obtain dispersion liquid, AB-8 type macroporous adsorbent resin on dispersion liquid, first uses 2 times of column volume distilled water eluting, discards water liquid; The ethanol elution that the volumetric concentration continuing with 4 times of column volumes is 10%, discards eluent; The ethanol elution that is 80% by the volumetric concentration of 6 times of column volumes again, collected volume concentration 80% ethanol elution, decompression recycling ethanol is to without alcohol taste, and lyophilization, pulverizes, and obtains Fructus Rubi extract, and in Fructus Rubi extract, Fructus Rubi total flavones mass content is more than 57%.
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| CN106075007A (en) * | 2016-06-08 | 2016-11-09 | 河南中医学院 | The application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone |
| CN106138133A (en) * | 2016-09-21 | 2016-11-23 | 河南中医药大学 | The application in exhausted premenstrual syndrome medicine is treated in preparation of the Fructus Foeniculi total flavones |
| CN106138955A (en) * | 2016-09-21 | 2016-11-23 | 河南中医药大学 | The application in exhausted premenstrual syndrome medicine is treated in preparation of the Fructus Alpiniae Oxyphyllae total flavones |
| CN106236799A (en) * | 2016-09-21 | 2016-12-21 | 河南中医药大学 | The application in preparation treatment perimenopausal syndrome medicine of the Fructus Cnidii total flavones |
| CN106266476A (en) * | 2016-09-21 | 2017-01-04 | 河南中医药大学 | The application in exhausted premenstrual syndrome medicine is treated in preparation of the Semen Herba Alii fistulosi total flavones |
| CN106309543A (en) * | 2016-09-21 | 2017-01-11 | 河南中医药大学 | Application of psoralen total flavonoid in preparation of medicaments for treating perimenopausal syndromes |
| CN106420891A (en) * | 2016-09-21 | 2017-02-22 | 河南中医药大学 | Application of folium cortex eucommiae general flavone in preparation of medicine for treating perimenopausal syndrome |
| CN106266476B (en) * | 2016-09-21 | 2019-10-29 | 河南中医药大学 | Green onion seed general flavone treats the application in premenopausal syndrome drug in preparation |
| CN106138133B (en) * | 2016-09-21 | 2019-10-29 | 河南中医药大学 | Fennel seeds general flavone treats the application in premenopausal syndrome drug in preparation |
| CN114209744A (en) * | 2022-01-11 | 2022-03-22 | 安徽农业大学 | Cream for treating mastitis of dairy cows and preparation method thereof |
| CN114209744B (en) * | 2022-01-11 | 2023-10-13 | 安徽农业大学 | A cream for treating milk cow mastitis, and its preparation method |
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