CN106075007A - The application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone - Google Patents
The application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone Download PDFInfo
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Abstract
The present invention relates to the application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone, can effectively solve the medicine for treatment problem of study on perimenopausal depressive disorder, method is, leek seed is ground into coarse powder, adds petroleum ether reflux, extract, defat, discards petroleum ether；Leek seed medicinal residues volatilize petroleum ether, add soak with ethanol, reflux, extract, and extracting solution decompression recycling ethanol, to without alcohol taste, adds distilled water dispersion, obtains sample liquid；AB 8 type macroporous adsorbent resin in sample liquid, successively with distilled water, mass concentration 10% ethanol, mass concentration 80% ethanol elution, collects 80% ethanol elution, and decompression recycling ethanol, to without alcohol taste, adds equal-volume petroleum ether extraction, discards petroleum ether；Flinging to organic solvent, water layer lyophilization, pulverize, obtain leek seed total flavone, this leek seed total flavone is effective to the medicine of preparation treatment study on perimenopausal depressive disorder, it is achieved the application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone.
Description
Technical field
The present invention relates to medicine, a kind of leek seed total flavone is in preparation treatment study on perimenopausal depressive disorder medicine
Application.
Background technology
Leek seed comes from " Mingyi Bielu " the earliest, doing for liliaceous plant Folium Allii tuberosi Allium tuberosum Rottler
Dry mature seed, Henan is its main producing region；The most edible scalable human body Liver and kidney lacks, and leek seed lacks for Liver and kidney, kidney yang
Void declines caused sexual impotence, and soreness of the waist and knees is painful.Semen Allii Tuberosi has invigorating the liver and kidney, warm waist knee joint, the function of invigorating kidney-YANG.Can single use, or and Tu
SIZI, Fructus Psoraleae, the product of the kidney invigorating and YANG supporting such as Herba Epimedii are employed together, for the seminal emission caused by instability of kidney QI, frequent micturition, the enuresis, band
The dilutest.Semen Allii Tuberosi has the kidney invigorating warming YANG, the effect of controlling nocturnal emission with astringent drugs reducing urination, as " your daughter wants side " alone this product is ground into powder or makees honeyed pill clothes, suffers from a deficiency of the kidney
Seminal emission leukorrhagia.Controlling seminal emission, also normal and Os Draconis, Semen Cuscutae compatibility, such as " red platform jade case " SIMIAO WAN." Tibetan side of Wei Shi family " is then with Semen Allii Tuberosi
Compatibility Fructus Psoraleae, the kidney invigorating reducing urination medicine such as Fructus Alpiniae Oxyphyllae, control kidney and deficiency-cold in the urinary bladder, frequent micturition.
Study on perimenopausal depressive disorder is a kind of dysthymic disorder, and the several years before menopause have occurred slight emotion in many women
Obstacle.Mostly a couple of days before menstrual onset is appeared periodically in, and natural remission, i.e. " premenstrual syndrome " after menstrual onset.
The initiation potential of these patient's study on perimenopausal depressive disorders may increase, and drastically influence the healthy of patient.
Though the existing multi-medicament having treatment study on perimenopausal depressive disorder, but for various reasons, it uses and curative effect not to the utmost
People's will, therefore, improvement and innovation on treatment study on perimenopausal depressive disorder medicine are that those skilled in the art research and solve always
Technical problem, then can so far there are no is publicly reported by leek seed total flavone preparation treatment study on perimenopausal depressive disorder medicine.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, it is yellow that the purpose of the present invention is just to provide a kind of leek seed total
Ketone application in preparation treatment study on perimenopausal depressive disorder medicine, the medicine for treatment that can effectively solve study on perimenopausal depressive disorder is asked
Topic.
The present invention solves technical scheme, and a kind of leek seed total flavone is in preparation treatment study on perimenopausal depressive disorder medicine
Application, described leek seed total flavone is that leek seed is ground into coarse powder, adds petroleum ether reflux, extract, defat, discards oil
Ether；Leek seed medicinal residues volatilize petroleum ether, add soak with ethanol, reflux, extract, and extracting solution decompression recycling ethanol, to without alcohol taste, adds distillation
Water-dispersible, obtain sample liquid；AB-8 type macroporous adsorbent resin in sample liquid, successively by distilled water, mass concentration 10% ethanol, quality
Concentration 80% ethanol elution, collects 80% ethanol elution, and decompression recycling ethanol, to without alcohol taste, adds equal-volume petroleum ether extraction,
Discard petroleum ether；Fling to organic solvent, water layer lyophilization, pulverize, obtain leek seed total flavone.
This leek seed total flavone is effective to the medicine of preparation treatment study on perimenopausal depressive disorder, it is achieved leek seed total flavone exists
Application in preparation treatment study on perimenopausal depressive disorder medicine.
Detailed description of the invention
Below in conjunction with concrete condition, the detailed description of the invention of the present invention is elaborated.
The present invention, in being embodied as, is realized by following methods.
The application in preparation treatment study on perimenopausal depressive disorder medicine of a kind of leek seed total flavone, described leek seed total is yellow
Ketone is, leek seed was ground into the coarse powder of 20-30 mesh, and petroleum ether (the boiling range 60-90 DEG C) backflow adding 10 times of weight of coarse powder carries
Take 1h, defat, discard petroleum ether；Coarse powder adds commensurability petroleum ether reflux, extract, 1h, defat again, discards petroleum ether；Leek seed medicinal residues
Volatilize petroleum ether, add soak with ethanol 0.5h of 10 times of weight mass concentrations 80% of coarse powder, reflux, extract, 2 times, each 1h, filter,
Merging filtrate, decompression recycling ethanol, to without alcohol taste, adds distilled water and is dispersed to be equivalent to, containing crude drug amount 0.3g/ml, obtain sample liquid；Sample
AB-8 type macroporous adsorbent resin on product liquid, first with 2 times of column volume distilled water eluting, discards water liquid；Continue by 4 times of column volumes, quality
Concentration 10% ethanol elution, discards eluent；Again with 6 times of column volumes, mass concentration 80% ethanol elution, collect 80% ethanol and wash
De-liquid, decompression recycling ethanol, to without alcohol taste, adds equal-volume petroleum ether extraction 1 time, discards petroleum ether；Fling to organic solvent, water layer
Lyophilization, pulverizes, obtains leek seed total flavone.
Leek seed total flavone of the present invention, through site test, can be effectively used for the medicine of preparation treatment study on perimenopausal depressive disorder,
Realizing the application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone, relevant testing data is as follows:
1. materials and methods
1.1 experiment material
1.1.1 laboratory animal
KM mice, SPF level, female, 22～25g, Lukang Medical Co., Ltd., Shandong；The animal quality certification number:
37005400000048；Experimental Ethical is numbered: DWLL16020050；Henan College Of Traditional Chinese Medicine animal experimental center credit number:
SYXK (Henan) 2015-0005.
1.1.2 Experimental agents
Leek seed total flavone；
Geng Nian An Capsules, Shanxi Yellow River Traditional Chinese Medicine Co., Ltd. produces, lot number 150102；Soybean isoflavone dimension E soft capsule, prestige
Sea purple light biotechnology development corporation, Ltd. produces, lot number 14100301.
1.1.3 experiment reagent
Chloral hydrate, Ke Miou chemicals development centre, Tianjin, lot number 20140807；Sodium carboxymethyl cellulose, sky
Heng Xing chemical reagent Manufacturing Co., Ltd of Jinshi City, lot number 20140728；Benzylpenicillin sodium for injection, Huabei Pharmaceutic Co., Ltd
Produce, lot number F4046509；0.9% sodium chloride injection, limited point of Cologne, Henan Pharmaceutical department, lot number C2015041601-1；First
Aldehyde solution (analytical pure), Chemical Co., Ltd. in pairs of Yantai City, lot number 20150401；Ethanol: the big disinfectant preparation in Xinxiang City three has
Limit company；Product batch number 20140905；Mice E2ELISA detection kit, R&D company, lot number 20151101A；Mice T
ELISA detection kit, R&D company, lot number 20151101A；Mice 5-HT ELISA detection kit, R&D company, lot number
20151101A；Mice DA ELISA detection kit, R&D company, lot number 20151101A；Mice NE ELISA detectable
Box, R&D company, lot number 20151101A.
1.1.4 experimental apparatus
Electronic scale, Shanghai Minqiao Medical Apparatus and Instruments Co., Ltd. produces, model JY601；Electronic analytical balance, Ao Haosi (on
Sea) company's production, model AR1140/C；High speed tabletop centrifuge, Anting Scientific Instrument Factory, Shanghai produces, model TGL-168；Little
Mus autonomic activities tester, Chengdu TME Technology Co., Ltd. produces, model ZZ-6；Mice keeps away dark instrument, and Chengdu Tai Meng science and technology has
Limit company produces, model BA-200；Outstanding tail tester, Chengdu TME Technology Co., Ltd., model TS-200；Electric heating constant temperature water-bath
Pot, Shanghai Yiheng Scientific Instruments Co., Ltd produces, model HWS12；Adjustable pipette, the limited public affairs of the vigorous analytical tool of Shanghai thunder
Department；Microplate reader, BIO-RAD company of the U.S. produces, model 680；Electric microscope, OLYMPUS company of Japan, model: BX61.
1.2 experimental technique
1.2.1 modeling and administration
Modeling method: take body weight 22～25g female KM mice 84, takes 10 at random and is only used as blank group, do sham-operation
Processing, remaining mice makes climacteric model.After mouse weights pneumoretroperitoneum injects 10% chloral hydrate (0.03ml/10g) anesthesia
Abdomen position is fixed, then from mouse back most end rib, midaxillary line and outside spinal column about 1cm infall unhairing (use animal
Special hairclipper unhairing), cut skin and dorsal muscles about 0.5～1cm after iodine disinfection, in the otch visual field, a visible milky is shinny
Liparitosis, ovary i.e. embeds wherein.Clamp outside liparitosis pull-out otch gently with pincet, fractionation of fatty group, the most visible one
The thin-line-shaped irregular ovary in yellowish red color of group.First fallopian tube under ovary (including fat) fine rule is ligatured during clip, then pluck
Except ovary, after postoperative normal saline cotton balls wiping, take advantage of a situation and cornua uteri is put back in abdominal cavity, suture muscles and skin, pluck with method
Except bilateral ovaries.Careful postoperative is raised, and intramuscular injection penicillin 250,000 u/kg (every 0.02mL) is in case infecting, continuous 3d, often
It is 1 time.Starting by only carrying out mouse vagina plate coating checking after operation 5d, every day 1 time, continuous 5d, to determine that ovary is the most complete
Excision.Smear presents the mice emotionally reacted and is discarded, and selects 60 castration mices to be completely uniformly divided into 6 groups at random for real
Test use, respectively model group, Geng Nian An Capsules group, soybean isoflavone soft capsule group, large, medium and small dosage leek seed total flavone group.
After being administered 5d, 5/cage of naive mice is raised, and normal diet is drunk water, and does not give any stimulation, and 6 groups of modeling type is
1 mice raised by every cage, gives different stimulated at random every day.6 kinds of stress factors are applied in 18d by random method: 1. moist
Bedding and padding (1g bedding and padding, 1ml water), 2. frozen water swimming (4 DEG C, 5min), 3. heat stress (45 DEG C, 5min), 4. illumination (24h) all night,
5. fasting (24h), 6. prohibits water (24h),.Giving a kind of stimulation every day at random, every kind of stimulation can not occur continuously, continuous 18d.
Make up a prescription: 0.5%CMC compound method: weigh sodium carboxymethyl cellulose 4g, be made into 800ml with distilled water.
(1) large, medium and small dosage leek seed total flavone group dosage is respectively 300mg kg-1、150mg·kg-1、
75mg·kg-1(being equivalent to the 30 of quantity, 15,7.5 times, be administered volume 0.1ml/10g).Collocation method: take Folium Allii tuberosi respectively
Seed total flavones 4,2,1, be first suspended in a small amount of 0.5%CMC solution, constant volume is to 40ml, mixing the most again, is required
The large, medium and small dose suspension of leek seed total flavone.
(2) Geng Nian An Capsules (675mg kg-1, concentration 67.5mg mL-1, be equivalent to 15 times of quantity)；Configuration
Method: take Geng Nian An Capsules 9, is first suspended in a small amount of 0.5%CMC solution, and constant volume is to 40ml, mixing the most again, is institute
Need Geng Nian An Capsules suspension.
(3) soybean isoflavone dimension E soft capsule (250mg kg-1, concentration 25mg mL-1, be equivalent to the 15 of quantity
Times)；Collocation method: take soybean isoflavones capsules 2, is first suspended in a small amount of 0.5%CMC solution, and constant volume is to 40ml the most again,
Mixing, is required soybean isoflavone suspension.
Storage method: cold preservation, used time water proof warms
Medication: each treated animal gives relative medicine on the 10th day in Post operation.Geng Nian An Capsules group gavages GENGNIANAN glue
Capsule suspension 675mg kg-1, soybean isoflavone soft capsule group gavages soybean isoflavone soft capsule suspension 250mg kg-1,
The large, medium and small other group of dosage leek seed total flavonoid component gavages large, medium and small dosage leek seed total flavone 300mg kg-1、
150mg·kg-1、75mg·kg-1.Blank group and model group gavage same volume distilled water respectively, every day gastric infusion 1 time, continuously
It is administered 30d.
1.2.2 observation item and detection method
2h after last is administered, mice takes blood, measures the content of E2, T, DA, 5-HA, NE in serum；Then mice is put to death,
Extract thymus, spleen, uterine cancer cell, weigh its weight in wet base and calculate organ index, then taking brain, cut half, prepare cerebral tissue
Homogenate, exempts from method with enzyme and measures the content of monoamine neurotransmitter 5-HT, DA in brain tissue homogenate.By thymus, spleen, uterus, brain
Tissue is fixed in 10% formalin solution, and HE dyes, and observes the tectology change of each group.Under surveying with Immunohistochemical Method
In thalamus, hypophysis, uterus, ER expresses, and in hypothalamus, hypophysis, AR expresses；
1.2.2.1 forced swim test test
Chronic stress stimulates and within the 1st day, measures each group of mice 2-6 minute dead time in 6 minutes after terminating.(little by each group
Mus puts into the beaker of 2000mL, and 25 DEG C of animals of water temperature are in addition to keeping floating required necessary minimum movement, and limbs stop fortune
Dynamic be designated as motionless, in record 6min the 2nd～6min dead time summation.)
1.2.2.2 autonomic activities test
Chronic stress stimulate terminate after within the 2nd day, measure autonomic activities number of times in each group of mice 5min.
1.2.2.3 darkness avoidance test
Chronic stress stimulates the 3rd day after terminating, and puts in determination box by mouse tail facing to the osculum entering darkroom, enters
Row training, redeterminates after 24h in mice first enters into incubation period in darkroom and 5min and enters the number of times shocked by electricity in darkroom.
1.2.2.4 tail-suspention test
Chronic stress stimulates and within the 5th day, measures each group of mice 2-6 minute dead time in 6 minutes after terminating.
1.2.3 kit measurement method
The acquisition of serum specimen: use without pyrogen and endotoxic test tube, avoid any stimulation during taking blood, prevent
Haemolysis；After collecting blood, after 3000 leave the heart 10 minutes, by separation the most careful to serum and erythrocyte.
Mouse E2 (E2), testosterone (T), 5-hydroxy tryptamine (5-HT), dopamine (DA) ELISA detection kit mensuration side
Method, refers to description.
1.2.4 statistical procedures method
Data analysis SPSS17.0 medical statistics bag carries out the statistical procedures of data information, measurement data average
± standard deviationRepresenting, comparing employing one factor analysis of variance between each group, the neat person of variance test uses LSD method, heterogeneity of variance person
Checking by Games-Howell method, ranked data use Ridit inspection.
2 results
2.1 impacts on perimenopausal depression model mice autonomic activities
Test each group of mice autonomic activities test result, be shown in Table 1.
The impact on perimenopausal depression model mice autonomic activities of the table 1 leek seed total flavone
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, with blank group ratio, the activity of model group mice, number of times of standing substantially reduce (P < 0.01), instead
Reflect study on perimenopausal depressive disorder model mice the curious degree of fresh ambient is reduced.With model group ratio, large, medium and small dosage fragrant-flowered garlic
Semen Allii Tuberosi total flavones, soybean isoflavone soft capsule, Geng Nian An Capsules be remarkably improved the movable number of times of mice and number of times of standing (P <
0.01)。
2.2 on perimenopausal depression model mice darkness avoidance test Middle latency and are affected by number of shocks
Test each group of mice darkness avoidance test test result, be shown in Table 2.
Perimenopausal depression model mice is kept away the impact of dark incubation period and number of shocks by table 2 leek seed total flavone
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, with blank group ratio, substantially reduce, dramatically increased by number of shocks the incubation period of model group mice
(P < 0.01), reflects perimenopausal depression model mice memory and reduces.With model group ratio, each administration group all can significantly extend
The incubation period (P < 0.01) of mice, soybean isoflavone soft capsule group, GENGNIANAN group, heavy dose of leek seed total flavone group can be notable
Reduce mice by number of shocks (P < 0.01).
2.3 on perimenopausal depression model mice forced swimming and the impact of outstanding tail dead time
Test each group of mouse forced swimming test and outstanding tail dead time test result, be shown in Table 3.
Table 3 leek seed total flavone is on perimenopausal depression model mice forced swimming and the impact of outstanding tail dead time
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, and blank group ratio, forced swimming and the outstanding tail dead time of model group mice all dramatically increase
(P < 0.01), reflects study on perimenopausal depressive disorder model mice and raises the desperate degree of adverse environment.With model group ratio, big,
Middle dosage leek seed total flavone, soybean isoflavone soft capsule, that Geng Nian An Capsules can significantly substantially reduce mouse forced swimming test is motionless
Time and outstanding tail dead time (P < 0.01).
2.4 on the impact of monoamine neurotransmitter level in study on perimenopausal depressive disorder model mice brain
5-HT, DA level in each group mouse brain, the results are shown in Table 4.
Table 4 leek seed total flavone is on the impact of 5-HT, DA level in perimenopausal depression model mice brain
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, and blank group ratio, 5-HT, DA level in model group Mice Homogenate all significantly reduce (P <
0.01), illustrate to make the success of murine chronic Stress Depression Model.With model group ratio, heavy dose of leek seed total flavone, soybean isoflavone
5-HT and the DA level (P < 0.01) that soft capsule, Geng Nian An Capsules are remarkably improved in brain homogenate；Middle dosage leek seed total flavone
The 5-HT (P < 0.01) being remarkably improved in brain homogenate, hence it is evident that improve DA level (P < 0.05) in brain homogenate.
2.5 impacts on perimenopausal depression model mice organ index
Respectively organizing mouse thymus, spleen, uterus index, result is shown in 5.
Table 5 leek seed total flavone is on perimenopausal depression model mice thymus, spleen, the impact of uterus index
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, and blank group ratio, model group thymus index, index and spleen index, uterus index all significantly reduce (P <
0.01), illustrate that the atrophy of thymus, spleen and uterine cancer cell occurs in study on perimenopausal depressive disorder mouse model.With model group ratio, big,
Middle dosage leek seed total flavone, Geng Nian An Capsules, soybean isoflavone soft capsule be remarkably improved the thymus of animal pattern, spleen,
Uterus index (P < 0.01).
2.6 impacts on perimenopausal depression model mice Serum Sex Hormones
E in each group mice serum2, T content, the results are shown in Table 6.
Table 6 leek seed total flavone is to E in perimenopausal depression model mice serum2, the impact of T content
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As can be seen from the above table, with blank group ratio, E in model group mice serum2, T level be remarkably decreased (P < 0.01), say
Sex hormone level in bright mice serum is disorderly, and perimenopausal depression mouse model replicates successfully.With model group ratio, big or middle dose
Amount leek seed total flavone group, Geng Nian An Capsules, soybean isoflavone soft capsule group all can significantly increasing serum E2, T level (P <
0.01)。
2.7 impacts on SABC index
Climacteric is merged the expression of ER, AR in hypophysis and hypothalamus in depression model Mouse Uterus, hypophysis, hypothalamus
The impact expressed, is shown in Table 7, table 8.
The impact that perimenopausal depression model mice hypophysis, uterus, hypothalamus ER are expressed by table 7 leek seed total flavone is (flat
All optical density)
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
(average light is close in the impact that perimenopausal depression model mice hypophysis, hypothalamus AR are expressed by table 8 leek seed total flavone
Degree)
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, compare with blank group, model mice respectively organize the expression of ER, AR all significantly reduce (P <
0.01), illustrate to extract completely climacteric merging depressed mouse model body linked groups caused by ovary merging chronic stimulation to divide
The sex hormone receptor of cloth decreases, thus reduces the biological effect of estrogen and androgen.Compare with model group, heavy dose of leek seed
Total flavones, Geng Nian An Capsules and soybean isoflavone soft capsule can significantly raise ER in uterus, hypophysis, hypothalamic expression (P <
0.01), the notable AR that raises is in hypophysis, hypothalamic expression (P < 0.01).
2.8 impacts on perimenopausal depression model mice organ-tissue form
Test each group of Mouse Uterus, hypothalamus, thymus, spleen pathologic examination result as follows, pathological photograph
2.8.1 climacteric is merged the impact of depression model endometrium of mouse thickness
The impact on perimenopausal depression model mice endometrium thickness of the table 9 leek seed total flavone
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
As seen from the above table, with blank group ratio, model group endometrium of mouse thickness is remarkably decreased (P < 0.01), has illustrated
The notable metratrophia of climacteric mouse model caused by full excision ovary.With model group ratio, heavy dose of leek seed total flavone, Semen sojae atricolor
Isoflavone soft capsule group, Geng Nian An Capsules group all can significantly raise the thickness (P < 0.01) of endometrium.
2.8.2 climacteric is merged depression model mouse thymus, the impact of spleen tissue form
Use micrometer, each group every Mouse Thymic Cortex thickness of experiment and the narrowest place are measured its thickness and try to achieve all
Number；Then the lymphocyte number recording thymic cortex thickness and the narrowest place being pressed on baseline with the baseline of micrometer is tried to achieve all
Number；
The baseline using micrometer falls on splenic nodule, measures the splenic nodule thickness of both sides centered by central artery respectively
Size, seeks mean；Calculate the number of the lymphocyte number of both sides on baseline simultaneously, seek mean；
Table 10 leek seed total flavone is on perimenopausal depression model mice thymus, the impact of spleen pathological change
Note:ΔΔP < 0.01, represents and compares with blank group；* P < 0.05, * * P < 0.01, represents and compares with model group
Can be seen that from upper table, and blank group ratio, model group Mouse Thymic Cortex thickness, splenic nodule volume substantially reduce (P <
0.01), illustrate that mice makes thymus, spleen volume atrophy after perimenopausal depression model.With model group ratio, big or middle dosage Folium Allii tuberosi
Seed total flavones, soybean isoflavone soft capsule group, Geng Nian An Capsules group can significantly thicken thymic cortex and splenic nodule volume (P <
0.01)。
3. conclusion
Leek seed total flavone merges depression model to mice climacteric has fine improvement result, leek seed total flavone to show
Write and improve the movable number of times of mice and number of times of standing；Can significantly extend the incubation period of mice, can substantially reduce mice is subject to electricity
Hit number of times.Leek seed total flavone can significantly substantially reduce mouse forced swimming test dead time and outstanding tail dead time.Leek seed total
5-HT and the DA level that flavone is remarkably improved in brain homogenate.Leek seed total flavone is remarkably improved the thymus of animal pattern, spleen
Dirty, uterus index.Leek seed total flavone can significantly increasing serum E2, T level.Leek seed total flavone significantly raise ER in uterus,
Hypophysis, hypothalamic expression, the notable AR that raises is in hypophysis, hypothalamic expression.Leek seed total flavone can significantly raise intrauterine
The thickness of film, can significantly thicken thymic cortex and splenic nodule volume, is effective to the medicine of preparation treatment study on perimenopausal depressive disorder,
Open up the new way on treatment study on perimenopausal depressive disorder medicine, be the innovation on medicine, there is the using value of reality.
Claims (1)
1. the application in preparation treatment study on perimenopausal depressive disorder medicine of the leek seed total flavone, described leek seed total flavone
It is that leek seed was ground into the coarse powder of 20-30 mesh, adds petroleum ether reflux, extract, 1h of 10 times of weight of coarse powder, defat, discard stone
Oil ether；Coarse powder adds commensurability petroleum ether reflux, extract, 1h, defat again, discards petroleum ether；Leek seed medicinal residues volatilize petroleum ether, add coarse powder
Soak with ethanol 0.5h of 10 times of weight mass concentrations 80%, reflux, extract, 2 times, each 1h, filter, merging filtrate, recovered under reduced pressure second
Alcohol, to without alcohol taste, adds distilled water and is dispersed to be equivalent to, containing crude drug amount 0.3g/ml, obtain sample liquid；In sample liquid, AB-8 type macropore is inhaled
Attached resin, first with 2 times of column volume distilled water eluting, discards water liquid；Continue with 4 times of column volumes, mass concentration 10% ethanol elution, abandon
Remove eluent；Again with 6 times of column volumes, mass concentration 80% ethanol elution, collecting 80% ethanol elution, decompression recycling ethanol is to nothing
Alcohol taste, adds equal-volume petroleum ether extraction 1 time, discards petroleum ether；Fling to organic solvent, water layer lyophilization, pulverize, obtain leek seed
Total flavones.
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| CN111658724A (en) * | 2020-05-21 | 2020-09-15 | 安徽金龙源生物科技有限公司 | Preparation method and application of leek seed extract |
| RU2802977C1 (en) * | 2022-11-20 | 2023-09-05 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Use of patulythrin as an antidepressant |
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| CN111658724A (en) * | 2020-05-21 | 2020-09-15 | 安徽金龙源生物科技有限公司 | Preparation method and application of leek seed extract |
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