KR101817242B1 - Skin external composition containing Glehnia littoralis Fr. Schmidt extract - Google Patents

Abstract

The present invention relates to a gypsum- littoralis Fr. Schmidt extract, and more particularly, to a composition capable of providing a skin aging-preventing effect by containing a ginseng extract.

Description

[0001] The present invention relates to a skin external composition containing Glehnia littoralis Fr. Schmidt extract}

The present invention relates to a gypsum- littoralis Fr. Schmidt extract, and more particularly, to a composition capable of providing a skin aging-preventing effect by containing a ginseng extract.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the content of ultraviolet rays reaching the surface of the sunlight increases and environmental pollution is further intensified, free radicals and active noxious oxygen are increased, so that the thickness of the skin decreases, the wrinkles increase, the elasticity decreases Skin color is grayish, skin troubles occur frequently, and spots, freckles and black blots also increase.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid and glycoprotein, which constitute the skin, are changed or decreased, and oxidative stress due to free radicals and active noxious oxygen is experienced. In addition, most of the cells constituting the skin undergo aging or ultraviolet rays, Cox-2 (cyclooxygenase), an enzyme that produces a proinflammatory cytokine known to cause inflammation, And the biosynthesis of matrix metalloproteinase (MMP), which is an enzyme that degrades skin tissue, is increased by these inflammatory factors, and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase) Respectively. In other words, there is a decrease in cell activity due to natural endogenous aging and a decrease in the biosynthesis of substrate materials due to microinflammation, an increase in stress due to various harmful environments, and an increase in active coral species caused by sunlight As the degradation and denaturation are accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, it is a reality that many researches have been conducted on the active ingredients which can prevent and improve such aging phenomena.

Accordingly, the inventors of the present invention have found that the extract of Guanxifury mushroom extract can provide an anti-aging effect, and completed the present invention.

Accordingly, an object of the present invention is to provide a composition for external application for skin, which can contain an extract of Aspergillus oryzae to provide an anti-aging effect.

In order to accomplish the above object, the present invention provides a composition for external application for skin, comprising an extract of Aspergillus species as an active ingredient.

The present invention also provides a composition for external application for skin for anti-aging, which comprises a ginseng extract as an active ingredient.

The composition of the present invention can provide an excellent anti-aging effect by containing a ginseng extract.

The composition for external application for skin of the present invention is characterized in that, littoralis Fr. Schmidt ) extract as an active ingredient.

Glehnia littoralis Fr. Schmidt ) is a perennial herb that grows in the sandy beach of Korea. The growing environment is attached to the sandy beaches and coastal cliffs where sunlight enters well. The height is 5 ~ 20㎝, and the leaf length is 10 ~ 20㎝. Its roots are yellow in color and extend vertically in the ground. It is used as medicinal herb and is used for edible purposes.

The ginseng extract of the present invention can be used not only as a leach solution obtained by leaching and transferring in a windshield, but also as a concentrate obtained by partially or entirely concentrating the leach solution or a stagnant product prepared by re-drying the concentrate, And all of the plants themselves as well as chemical substances contained in the windshield and exhibiting the main effect. In the present invention, an extract from all parts of the stem, root, leaf, flower, and the like can be used, and the extract is not limited to any specific part.

A known method can be used as a method for producing the pseudo windproof extract used in the present invention. For example, after finely dividing the windshield of the present invention and drying it, water or an organic solvent is added, and the mixture is refluxed and extracted. After filtration and centrifugal separation, the residue and the filtrate are separated and the separated filtrate is concentrated under reduced pressure, An extract can be obtained. The organic solvent which can be used in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water. Preferably, 100% water, 70% Or Butylene Glycol is used. In this case, the extraction temperature is preferably 18 to 80 ° C in the case of water, and 18 to 40 ° C in the case of 70% ethanol and butylene glycol, and the extraction time is preferably 6 to 168 hours. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed. After obtaining the extract using a solvent, the extract can be obtained by cooling, heating and filtering at room temperature by a conventional method known in the art, or the solvent can be further evaporated, spray dried or freeze-dried.

The composition for external application for skin according to the present invention may contain 0.001 to 50% by weight of the above-described guar gangbang extract, based on the total weight of the composition. If the content of the extract is less than 0.001% by weight, the efficacy and the effect of the extract are insufficient. If the content is more than 50% by weight, skin safety or shape problems may occur.

The composition of the present invention can be used as a composition for external application for skin for anti-aging, which is excellent in improving skin elasticity and improving wrinkles.

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of ionic fibrin dispersions, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin aging improving effect.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Preparation of extract

After shredding, shade and shade were extracted by refluxing three times for 24 hours using 70% ethanol as an extraction solvent, followed by cooling and filtering with a Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 45 ° C or lower.

[Test Example 1] Elastase activity inhibition assay

The inhibition of elastase activity of pseudomonas aeruginosa extract was measured in comparison with phosphoramidon. The elastase and substrate used were commercially purchased (Cat. No. E0127) from Sigma-Aldrich, USA, and the bark extract of Example 1 was used.

The elastase activity inhibitory activity was tested by the following test method.

L of Tris-HCl buffer solution (pH 8.0) was prepared in a 96-well plate, and 50 μL of 20 μg / mL elastase type III solution was mixed so as to be 10 ppm of the above-described A. niger virus extract of Example 1. 10 ug / ml of phosphoramidon was used as a positive control and distilled water was used as a negative control group. Thereafter, 100 μL of 0.4514 mg / mL of N-SUCCINYL-ALA-ALA-ALA-p-NITROANILIDE prepared as the buffer solution was added and reacted at 25 ° C for 15 minutes. After completion of the reaction, the absorbance at a wavelength of 415 nm was measured. A blank test was carried out in the same manner and corrected.

The method for calculating the activity of inhibiting the activity of the elastase is shown in the following Equation 1, and the results are shown in Table 1 below.

A: absorbance at wavelength 415 nm in the absence of the test substance and addition of the enzyme

B: absorbance at a wavelength of 415 nm in the absence of the test substance and no enzyme addition

C: Absorbance at wavelength 415 nm in addition of test substance and enzyme addition

D: Absorbance at wavelength 415 nm in addition of test substance and enzyme-free addition

compound % Inhibition Untreated group 0 Phosphoramidone 35 Example 1 27

As shown in Table 1, the degree of inhibition of the elastase activity of the extract of the Aspergillus species was significantly inhibited as compared with that of the control group, and compared with the phosphoramidon, which is known as an inhibitor of elastase activity, It can be confirmed that the effect of inhibiting elastase activity of the extract of the present invention is excellent.

[Test Example 2] Collagenase (MMP-1)

The inhibitory activity of collagenase (MMP-1, substrate metalloprotease-1) production of the present invention was compared with that of retinoic acid.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing 2.5% fetal calf serum-containing DMEM (Dulbecco's Modified Eagle's Media) And incubated at 37 ° C in an incubator with CO _{2 of} 5% until it grew to about 80%. The ginseng extract was treated at a concentration of 10 μg / ml for 24 hours and then the cell culture was collected. For comparison, retinoic acid treated at the same concentration was used.

The degree of collagenase production in the cell culture was measured using a commercially available collagenase assay device (Amersham Pharmacia, USA, Catalog #: RPN 2610). First, the cell culture solution collected on a 96-well plate uniformly coated with primary collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. After 3 hours, the second collagen antibody bound to the chromophore was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the color development substance (3,3 ', 5,5'-tetramethylbenzidine, sigma) was added and color development was induced at room temperature for 15 minutes. When 1 M sulfuric acid was added to stop the color development reaction, the color of the reaction solution became yellow And the degree of yellowing was different according to the progress of the reaction.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of synthesis of collagenase was calculated by the following equation (2) Respectively. At this time, the reaction absorbance of the cell culture fluid collected from the non-treated group was used as a control.

compound Expression level (%) Untreated group 100 Retinoic acid 75 Example 1 70

As shown in Table 2 above, collagenase expression inhibitory effects of the extracts of Rhododendron spp. Are similar to those of retinoic acid, which is known as a collagenase expression inhibitor.

From these results, it can be confirmed that the present invention has the effect of inhibiting the substrate metalloprotease (MMP-1).

[Referential Example 1] Preparation of Formulation Example 1 and Comparative Formulation Example 1

Nutritive creams were prepared in a conventional manner according to the composition of Table 3 below (unit: wt%).

Compounding ingredient Formulation Example 1 Comparative Formulation Example 1 Purified water To 100 To 100 Windbreak 0.1 0 Vegetable hydrogenated oil 1.50 1.50 Stearic acid 0.60 0.60 Glycerol stearate 1.00 1.00 Stearyl alcohol 2.00 2.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 Cyclomedicone 6.00 6.00 Preservative, incense Suitable amount Suitable amount Triethanolamine 0.1 0.1

[Test Example 3] Confirmation of improving skin elasticity

In order to confirm the skin elasticity improvement effect in human, the formulations of Formulation Example 1 and Comparative Formulation Example 1 were evaluated as follows.

Twenty healthy women in the 30s to 40s were divided into two groups of 10 each for the two groups of Formulation Example 1 and Comparative Formulation Example 1, and the nutritional cream was applied to the face for one week for 12 weeks. Then, 575, C + K Electronic Co., Germany). The results are shown in Table 4 below. The results in Table 4 are reported as ΔR8 (R8 (left) -R8 (right)) of Cutometer SEM 575, where R8 values indicate the properties of viscoelasticity.

Experimental product Skin elasticity effect Formulation Example 1 0.15 Comparative Formulation Example 1 0.10

As shown in Table 4, Formulation Example 1 containing the extract of the present invention showed a greater skin elasticity than that of Comparative Formulation Example 1.

Therefore, it can be confirmed that the composition containing the extract of the present invention is very effective for improving skin elasticity.

[Test Example 4] Confirmation of skin wrinkle improving effect

Formulation Example 1 and Comparative Formulation Example 1 were used in order to confirm the wrinkle-reducing effect of the composition of the present invention in humans.

In order to confirm the effect of improving the wrinkles of Formulation Example 1 and Comparative Formulation Example 1, the following evaluation was made. Twenty healthy women in their 40s were divided into two groups of 10 each for Formulation Example 1 and Comparative Formulation Example 1 to apply the nutritional cream once a day for 12 weeks to the face, Were visually analyzed by viscometer (SV600, Courage + Khazaka electronic GmbH, Germany). The results are shown in Table 5 below. The values in Table 5 below are the average values obtained by subtracting the pre-application parameter values from the respective parameter values after 12 weeks of application.

After 8 weeks of use
Clinical outcome R1 R2 R3 R4 R5 Formulation Example 1 -0.30 -0.21 -0.14 -0.10 -0.09 Comparative Formulation Example 1 0.04 0.03 0.01 -0.01 0.00

R1: Difference between the maximum value and the minimum value of the contour line of the wrinkles

R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values

R3: the highest value of R1 divided by 5

R4: Average value obtained by subtracting the value of each apex and valley from the baseline of the wrinkle contour line

R5: Difference between the baseline of the wrinkle contour line minus the contour of each wrinkle

As shown in Table 5, it was found that the external preparation composition of Formulation Example 1 had a very excellent effect of improving skin wrinkles.

Claims (5)

A cosmetic composition for enhancing skin elasticity and improving skin wrinkles containing an extract of Glehnia littoralis Fr. Schmidt as an active ingredient. [2] The cosmetic composition according to claim 1, wherein the extract is in an amount of 0.001 to 50% by weight based on the total weight of the composition. The composition of claim 1 or 2, wherein the composition is in the form of a solution, a gel, a solid, a paste anhydrate, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule, The form of a non-ionic follicular dispersion; In the form of creams, skins, lotions, powders, ointments, sprays, or conical sticks; Or in the form of a foam or in the form of an aerosol composition further containing a compressed propellant; To improve skin elasticity and to improve skin wrinkles. delete delete