KR20090078084A - The cosmetic composition for improvement of the skin wrinkles containing adenosine and eucommia ulmoides extract - Google Patents

Abstract

A cosmetic composition for improving antiwrinkle, which contains Eucommia ulmoides OLIVER extract and adenosine is provided to promote collagen synthesis and synergically improve antiwrinkle and elasticity. A cosmetic composition for antiwrinkle comprises 0.001-20.0 weight% of Eucommia ulmoides OLIVER extract and 0.001-5.0 weight% of adenosine as an active ingredient. The Eucommia ulmoides OLIVER extract is isolated using water, anhydrous or hydrous low alcohol of 1-4 carbon atoms, mixture solvent of low alcohol and water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol. The Eucommia ulmoides OLIVER extract is pulverized through additional process such as compression distillation, freeze drying or spray drying. The Eucommia ulmoides OLIVER extract has the effects of cell proliferation promotion, collagen synthesis promotion, and collagenase inhibition. The composition is used in a form of skin softener, essence, lotion, cream, pack, gel, ointment, patch, or spray.

Description

The cosmetic composition for improvement of the skin wrinkles containing adenosine and Eucommia ulmoides extract}

The present invention relates to a cosmetic composition for improving skin wrinkles, comprising a worm extract and adenosine, more specifically, the worm extract promotes collagen synthesis in the skin and inhibits degradation to improve skin wrinkles and skin elasticity, and adenosine is a dermal layer. It relates to a cosmetic composition that can effectively prevent skin aging by promoting the synthesis of DNA and protein in synergistic effect to improve skin wrinkles and skin elasticity.

The skin is the largest organ of the human body, occupying about 16% of the body's volume and is important in protecting the body from many deadly harmful factors, such as temperature, humidity, and ultraviolet radiation, which are in direct contact with the external environment and are likely to enter the body. Act as a shield. However, as they age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111: 31-37 (1996)). Endogenous aging is a aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. Clinically, the elasticity is reduced, the skin texture is rough, deep wrinkles are formed, and pigments are deposited. Dermatol., 130: 87-95 (1994). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a marked decrease in elasticity.

Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, when the skin is exposed to harmful free radicals or free radicals, lipid peroxide is produced by oxidative action in the body and causes modification of various proteins constituting the skin. Meanwhile, collagen and elastic elastin proteins, which make up most of the dermis (about 70-80% of the total dry weight of the skin), are the major proteins produced by fibroblasts and are involved in the skin's mechanical firmness, tissue adhesion, and elasticity in the dermal layer. (Journal of the american academy of dermatology, 21: 610-613 (1989)). Collagen decreases with age and with increasing exposure to ultraviolet light. Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen types I and III constitute the constituents of the cytoplasm of the dermis, and collagen type IV is the main component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermal and dermal layers of the skin to regulate the permeation and transport of substances between the two layers, to control the differentiation of adjacent skin cells and to maintain the firmness of the skin. Collagen and elastin, the major structural components of the skin, are either denatured or reduced in biosynthesis by natural or external stimuli, thereby accelerating skin aging. In addition, the expression and activity of collagenase is also a major factor in accelerating skin aging.

Retinoic acid, epidermal growth factor (EGF), TGF (trans-forming growth factor), and carcinogenic factors (Cardinale G. et al., Adv.Enzymol., 41) , p. 425, 1974), animal placental proteins (JP8-231370), betulinic acid (betulinic acid (JP8-208424)), chlorella extracts (JP9-40523, JP10-36283, fibroblast proliferation) Known. In particular, epidermal growth factor acts as a potent promoter of epithelial cell, endothelial cell and fibroblast cell proliferation, and promotes epithelial cell migration and proliferation during epithelial cell defects. Retinol is also described in US Pat. Nos. 4,603,146 and 4,877,805 to effectively improve skin wrinkles on collagen synthesis and degradation inhibition. However, in the case of retinoic acid is unstable, there is a limit to the amount of use due to stability problems such as irritation, redness, etc. when applying the skin, chlorella extracts, etc. are insignificant effect, and can not effectively improve the skin function by promoting the collagen synthesis. Therefore, there is an urgent need for the development of new collagen synthesis promoters which are safe for living bodies and are more effective than conventional collagen synthesis ability promoting substances.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing the larvae extract and adenosine to improve skin wrinkles and promote skin elasticity to effectively prevent skin aging.

In order to easily achieve the above objectives as well as other objectives that are easily expressed, by preparing a cosmetic composition for improving skin wrinkles containing the larvae extract and adenosine, the larvae extract promotes the synthesis of collagen in the skin and inhibits degradation, thereby reducing skin wrinkles and skin It improves the elasticity and adenosine promotes the synthesis of DNA and protein in the dermal layer, thereby synergistically improving skin wrinkles and skin elasticity, thereby effectively preventing skin aging.

In other words, the inventors of the present invention, such as worm extract extracts to promote collagen synthesis in the skin and inhibit degradation to improve skin wrinkles and promote skin elasticity to prevent skin aging, adenosine is an intracellular component, so stability and sustainability during skin penetration The present invention has been found to be excellent and promotes the synthesis of DNA and protein in the dermis, thus helping to improve wrinkles.

The cosmetic composition for improving skin wrinkles containing the worm extract and adenosine according to the present invention improves skin wrinkles and skin elasticity by promoting the synthesis of collagen in the skin and inhibiting degradation, and adenosine synthesizes DNA and protein in the dermal layer. By promoting the synergistic effect on the improvement of skin wrinkles and skin elasticity can be effectively prevented skin aging.

The cosmetic composition for improving skin wrinkles according to the present invention is characterized by containing the worm extract and adenosine as active ingredients.

Eucommia ulmoides OLIVER, also known as worms, beetles, gold, two-sided or sugar worms, is a unique medicinal plant from ancient times, and is a deciduous tree belonging to the family Dermis, and is a single plant in genus 1 and 1. In China, it was called a fantasy herb because it was more precious than ginseng, and it has been known as a timber line. The larva is a male and female tree like a ginkgo tree, the larva is elliptical, similar to the elm leaf, and small white flowers bloom in spring, and when the bark is cut, a white rubbery fluid flows like a rubber tree. It is a plant that is resistant to pests, bacteria, and bacteria, grows well without spraying pesticides such as insect repellents, and hates acidic soils, but does not need to be used except for soil improvement.

The effects of the larvae have been mentioned from ancient literature. In the `` Main Herb '', the two larvae strengthen the muscles while watching the liver and gods, and if the larvae are eaten for a long time, the body becomes light and does not grow old. It is written that it gives birth, and the `` Cock-up Cluster '' states that larvae produce back and leg pain, protect the inside, and strengthen tendons and bones.

The larvae extract used in the present invention is preferably extracted by using the extraction solvent of the larvae leaves or the bark of the larvae stem. It can be obtained using a variety of extraction methods known in the art to which the present invention belongs, preferably, (a) water, (b) anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol) Etc.), (c) the mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol can be obtained as an extraction solvent. have.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvents, extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the extract of the present invention.

Extracts of the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, the larvae extract is effective to contain 0.001 to 20.0% by weight based on the total cosmetic composition. When the amount of the extract of the larvae is less than 0.001 weight, the effect of promoting the synthesis of collagen, inhibiting collagen degradation, and preventing skin aging of the cosmetic composition is insignificant, and when used in excess of 20.0% by weight, the synergistic effect due to the excessive input is not so economical. . Preferably from 0.01 to 10.0% by weight, more preferably from 0.1 to 5.0% by weight.

Adenosine is one of the elements that make up DNA, which is a gene, and is a compound that combines a base called adenine and a sugar called ribose, and is defined as a cosmetic ingredient that helps to improve skin wrinkles by the Food and Drug Administration. In addition, the attachment of three phosphates to sugar becomes ATP (adenosine 5'-triphosphate), the main source of energy in the cell, and adenosine is an important factor that plays a crucial role in all metabolism progressing smoothly in the body. Known. Because adenosine is an intracellular component, it is known to be highly stable and durable when penetrating the skin, and to help improve skin wrinkles by promoting DNA and protein synthesis in the dermis.

The present invention effectively improves the skin wrinkles by the synergistic effect of the worm extract and adenosine, the cosmetic composition containing the worm extract and adenosine can be prepared in any formulation commonly prepared in the art to which the present invention belongs, For example, flexible cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, pack, etc. are not particularly limited in the formulation or use of cosmetics It can be manufactured according to the purpose.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident. Compounding amount is shown by weight%.

Production Example  1: Preparation of Tobacco Extract

1 kg of the mixture of the leaves of the larvae and the chopped larvae stems in a weight ratio of 1: 1 was added 15 times by volume of water as an extraction solvent, heated at 70 ° C. for 1 hour, filtered, and then cooled to room temperature, and then cooled to 4 ° C. Aged at low temperature for 7 days and filtered and used as a larvae extract.

Experimental Example  1: Cytotoxicity Test of Tobacco Extract

In order to verify the primary safety as a raw material used in cosmetics, MTT test (Mossman T. (Ref .: Mossman T.) by culturing V79-4 cells (Chinese hamster, continuous cell line of lung tissue fibroblasts) to the worm extract of the present invention. 1983) Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays.Journal of Immunological Methods, 65, 55-63) was performed to test the cytotoxicity, the results are shown in Table 1.

sample ^a IC ₅₀ (%, W / V) Sodium Lauryl Sulfate (SLS) 0.005 Tofu extract 5.0

^a IC ₅₀ : Inhibitory Concentration 50, 50% cell killing concentration

As can be seen in Table 1, the IC ₅₀ value of the larvae extract according to the present invention is 5.0, it can be seen that the safety is excellent because the cytotoxicity 1000 times or more lower than sodium lauryl sulfate.

Experimental Example  2: skin cell proliferation effect

In order to examine the effect of larvae extract on skin cell proliferation, human normal fibroblasts (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate, and DMEM medium (Sigma) was incubated for 24 hours at 37 ℃. Subsequently, the experimental group was replaced with serum-free DMEM medium containing the final concentration of 0.01%, 0.05%, 0.1%, 0.5% and 1% and the control group replaced with serum-free DMEM medium containing no worm extract Was further incubated for 24 hours. Then, 10 μl each of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added thereto, followed by incubation for 4 hours. The medium was removed, shaken for 20 minutes by addition of 100 μl of DMSO solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured value was substituted into the following formula to calculate the cell proliferation effect, and the results are shown in Table 2 below.

Cell proliferation effect (%) = [(absorbance of experimental group-absorbance of control group) / absorbance of control group] x 100

Tofu extract (%) Absorbance (570nm) Cell proliferation effect (%) Control 0.631 - 0.01 0.765 21.2 0.05 0.854 35.3 0.10 0.981 55.5 0.50 1.064 68.6 1.00 1.245 97.3

As can be seen from Table 2, compared to the control group not treated with the larvae extract, the experimental group treated with the larvae extract shows a very high fibroblast proliferation effect.

Experimental Example  3: collagen synthesis promoting effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the experimental group in which the prepared worm extract was replaced with serum-free DMEM medium containing final concentrations of 0.01, 0.05, and 0.10% and the control group replaced with serum-free DMEM medium containing no worm extract for an additional 48 hours. Incubated. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen type I C-peptide (PICP) using a kit (Takara, Japan). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

Tofu extract (%) Collagen Synthesis (ng / 2 x 10 ⁴ ) Control 120 0.01 135 0.05 171 0.10 216

As can be seen from Table 3, the synthesis of collagen increased as the concentration of the worm extract of the present invention was increased, it can be seen that the worm extract of the present invention shows excellent collagen synthesis promoting effect.

Experimental Example  4 : Collagenase  Inhibitory effect

Inhibition of collagenase production of the larvae extract was measured. The test was performed using 2 x 10 ⁴ cells / well of human fibroblasts in 96-well microtiter plates containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum. It was added to, and incubated until 70 ~ 80% growth. In addition, after treatment for 24 hours for each concentration of the larvae extract, the cell culture was collected. The cultured cell culture solution was measured by using a commercially available collagenase measuring instrument (Amersham Pharmacia Co., Catalog #: RPN 2610). First, the cultured cells were placed in a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours. After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, a color-causing substance is added to cause color development at room temperature for 15 minutes. Then, 1M sulfuric acid is added to stop the reaction (color development). The color of the reaction solution is yellow and the degree of yellow color varies depending on the progress of the reaction. The absorbance of the yellowish 96-well plate was measured at 405 nm using an absorbometer and the degree of synthesis of collagenase was calculated. At this time, the reaction absorbance of the prepared cell culture medium of the group not treated with the composition was used as a control. As a result of measuring the inhibition of collagenase expression in the cell, it was confirmed that the substance inhibits collagenase expression as shown in Table 4 in vitro. Expression inhibition capacity is prepared by setting the synthetic ability of the untreated group to 100.

Tofu extract (%) Collagenase Expression (%) Control 100 0.01 85 0.05 78 0.10 72

From Table 4 it was confirmed that the collagenase expression inhibitory effect of the worm extract in the cell is very excellent compared to the control.

Production Example  2 : Example  And Comparative example

Examples and comparative examples were prepared in order to conduct a clinical trial on the skin wrinkle and elasticity improvement effect of the worm extract and adenosine-containing cosmetics of the present invention.

ingredient Example (% by weight) Comparative Example 1 (% by weight) Comparative Example 2 (% by weight) vaseline 7.0 7.0 7.0 Liquid paraffin 5.0 5.0 5.0 Beeswax 2.0 2.0 2.0 Polysorbate-60 2.0 2.0 2.0 Sorbitan sesquioleate 2.5 2.5 2.5 Squalane 3.0 3.0 3.0 Propylene glycol 6.0 6.0 6.0 glycerin 4.0 4.0 4.0 Triethanolamine 0.5 0.5 0.5 Carboxy Vinyl Polymer 0.5 0.5 0.5 Tocopherol Acetate 0.1 0.1 0.1 Tofu Extract 2.0 2.0 - Adenosine 0.04 - - Incense, preservative a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount

Experimental Example  5: skin wrinkle improvement

The skin wrinkle improvement effect of the cosmetic composition containing the larvae extract of the present invention and adenosine was measured in healthy women aged 35 to 50 years old. First, it was divided into three groups, Example A was applied to Group A, Comparative Example 1 to Group B, and the formulation of Comparative Example 2 to Group C was applied twice a day (morning and evening) on the face for three months. After 3 months, the degree of wrinkle improvement was evaluated by subject's questionnaire and image analysis of wrinkles.

The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in Table 6.

Image analysis of wrinkles was performed by taking a replica of the eye under the eye before the experiment was started, and replicas immediately after the experiment was completed at the same area under the eye. Was measured. The results of the measurement of wrinkle density by image analysis are shown in Table 7 as a reduction ratio against wrinkle density before use.

sample No improvement Minor improvements Moderate improvement A significant improvement Example One 2 3 4 Comparative Example 1 2 3 3 2 Comparative Example 2 8 2 - -

sample Wrinkle Density Reduction (%) Example 45 Comparative Example 1 35 Comparative Example 2 10

From Tables 6 and 7, it can be seen that the skin wrinkle improvement effect of the Example containing the larvae extract and adenosine was very excellent.

Experimental Example  6: skin elasticity effect

The skin elasticity enhancing effect of the cosmetic composition containing the tofu extract and adenosine of the present invention was measured. Thirty healthy women (average age 37 years old) of 20 years or older (mean age 37 years old) were divided into three groups under the conditions of the temperature of 25 degreeC, and the humidity of 75, The example of A group, the comparative example 1 to the B group, and the comparative example 2 of the C group After the formulation was applied twice a day (morning and evening) around the eyes for 12 weeks, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, Germany). The test results are described as R2 (R2 (12 weeks) -R2 (0 weeks)) values of the cutometer MPA580, where R2 values represent the properties of gross elasticity, and the results are shown in Table 8 below.

Experimental Formulation Skin elasticity effect Example 0.074 Comparative Example 1 0.065 Comparative Example 2 0.029

From Table 8, it was confirmed that the skin elasticity effect of the example containing the worm extract and adenosine increased compared to the comparative example.

Experimental Example  7: Stability test of formulation

The stability test for the cosmetics containing the tofu extract and adenosine of the present invention was carried out by the following method.

In order to test the stability of the cosmetics, Examples and Comparative Examples 1 and 2 were kept in an opaque glass container in a constant temperature bath at a constant temperature of 45 ° C. and stored for 12 weeks in a completely shaded refrigerator kept at a constant temperature of 4 ° C. After storing for 12 weeks in an opaque glass container, discoloration and deodorization, the degree of separation was measured by comparison results are shown in Table 9 below. At this time, the product discoloration, odor and separation degree was classified into six grades and evaluated.

0: No change 1: Very little separation (discoloration)

2: Slightly separated (discolored) 3: Slightly separated (discolored)

4: severely separated (discolored) 5: extremely severely separated (discolored).

division Formulation Stability Example Comparative Example 1 Comparative Example 2 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0 0 0 0 0 0 Deodorant 0 0 0 0 0 0 detach 0 0 0 0 0 0

As can be seen from Table 9, the Examples and Comparative Examples were stable at 45 ° C. with little discoloration, discoloration, and separation, indicating that the larvae extract and adenosine were present in a stable state in the formulation.

Hereinafter, on the basis of the results of the above experimental example, to present a cosmetic composition containing the tofu extract and adenosine of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation example  1: Flexible Cosmetic (Skin Lotion)

To the flexible composition according to the composition shown in Table 10 was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Tofu extract 0.5 Adenosine 0.04 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water Remaining amount

Formulation example  2: nutritional cosmetics ( Milk Croissant )

Nutrients according to the composition shown in Table 11 was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Tofu extract 1.0 Adenosine 0.04 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water Remaining amount

Formulation example  3: nutrition cream

To the nutrition cream was prepared according to the composition described in Table 12 in a conventional manner.

Compounding ingredients Content (% by weight) Tofu extract 2.0 Adenosine 0.04 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water Remaining amount

Formulation example  4 : Massage cream

Massage cream was prepared according to the conventional method in the composition described in Table 13.

Compounding ingredients Content (% by weight) Tofu extract 0.5 Adenosine 0.04 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water Remaining amount

Formulation example  5: pack

Packs were prepared according to conventional methods in the compositions shown in Table 14 below.

Compounding ingredients Content (% by weight) Tofu extract 1.0 Adenosine 0.04 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water Remaining amount

Claims (5)

Cosmetic composition for improving skin wrinkles, characterized in that it contains a worm extract and adenosine as an active ingredient. The cosmetic composition according to claim 1, wherein the worm extract is contained in an amount of 0.001 to 20.0% by weight and adenosine in an amount of 0.001 to 5.0% by weight based on the total weight of the composition. The method of claim 1, wherein the worm extract is extracted using water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol Cosmetic composition, characterized in that. The cosmetic composition according to claim 1, wherein the worm extract is effective in promoting skin cell growth, improving skin wrinkles and skin elasticity by promoting collagen synthesis, and inhibiting collagenase and a combination thereof. The cosmetic composition of claim 1, wherein the composition has a formulation of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.