KR20210091986A - Cosmetic composition having a whitening and moisturizing effect containing honey, schisandra fruit, Lycium chinese and green tea far-infrared mixed extract as active ingredients - Google Patents

Abstract

The present invention relates to a cosmetic composition having moisturizing and whitening effects, and more particularly, to a composition excellent in skin efficacy with a mixed extract obtained by processing honey, Schisandra chinensis, Lycium chinense and green tea by far-infrared extraction (F-IRE).

Description

Cosmetic composition having a whitening and moisturizing effect containing honey, schisandra fruit, Lycium chinese and green tea far-infrared mixed extract as active ingredients}

The present invention relates to a cosmetic composition having excellent skin moisturizing and whitening effects by containing a far-infrared mixed extract obtained through far-infrared extraction after drying and mixing honey, omija, goji berry and green tea in equal amounts.

The moisture in the stratum corneum of the skin decreases due to various factors such as damage caused by UV rays and external harmful environmental components, and the skin becomes dry and cracked as the surface becomes rough. When moisture is lost, dead skin cells are exfoliated and exposed to the outside, causing irritation and skin diseases. Dry skin weakens the immune function, making it easier to cause allergies such as itchiness and atopic dermatitis. Therefore, it is important to prevent moisture loss in the skin.

To prevent this, moisturizers are used in cosmetics, and ingredients often used include substances such as polyols such as glycerin, propylene glycol, 1,3-butylene glycol and polyethylene glycol as moisturizing ingredients, and lipid components such as ceramides as sealing ingredients. However, essential fatty acids and lipid complexes are used. However, when applied to the skin, polyol has a disadvantage in that it is sticky or feels soggy due to its inherent viscosity and physical properties. And, in the case of sealing components, there is a difficulty in disturbing the structure of the emulsified interfacial film in the emulsified formulation to inhibit stability, and there is a disadvantage in that it is not suitable for producing a transparent gel-like product.

Formulations containing lamellar liquid crystals, which have been studied a lot until recently, are known to have excellent moisture and moisturizing power due to advantages similar to those of the keratinous structure of the skin.

Modern people are exposed to the risk of disease contamination with various oxidizing substances generated in the body due to various factors such as environmental pollution and stress. A free radical or reactive oxygen species, which is one of the oxidizing material species, is an atom or group of atoms having odd electrons, and because it does not form a pair, it is very unstable, has high energy, and thus has a high reactivity. Examples of such free radicals include radicals such as superoxide anion radicals (O2-) and hydroxy radicals (OH-), non-radicals such as hydrogen peroxide (H2O2), and the like. It induces lipid peroxidation in vivo and damages all tissues including cells and skin. And it attacks DNA, causing genetic modification, etc., causing various diseases.

On the other hand, free radicals stimulate pigment-producing cells to produce melanin, causing pigmentation. In melanin, an abnormal inflammatory substance is expressed in keratinocytes by free radicals, and the expressed inflammatory substance activates melanocytes, which are melanin-producing cells, and promotes the synthesis of melanin in melanosomes. .

In addition, as studies on tyrosinase and TRP-1 (Tyrosinase related protein-1), TRP-2 enzymes and their roles are progressing, substances that inhibit their activity in melanin synthesis or have the effect of inhibiting the expression Research is actively underway.

Representative raw materials used as whitening cosmetics include arbutin, kojic acid, niacinamide, and hydroquinone. However, these raw materials have problems in efficacy and safety.

In order to solve this problem, we have recently developed natural bioactive materials from animals and plants to microorganisms and introduced them into cosmetics to maintain and improve the skin's own unique defense function, thereby alleviating skin irritation and using it safely, and at the same time whitening the skin. As it is necessary to develop a new raw material that has two effects of moisturizing and moisturizing, R&D is being promoted.

Therefore, in this study, it is essential to use raw materials derived from nature to solve the above problems and develop a cosmetic with excellent skin whitening and moisturizing effects.

Republic of Korea Patent Publication No. 10-1823036 (2018.01.31. Announcement), "Melanin production inhibitor and cosmetic composition containing the same"

The present invention is characterized by containing honey, omija, goji berry, and green tea far-infrared mixed extract as an active ingredient, thereby increasing moisture content and suppressing moisture loss in the epidermal layer, and does not cause side effects such as skin irritation, allergies and troubles, and skin whitening An object of the present invention is to provide a cosmetic composition having excellent moisturizing effect and greatly improved formulation stability.

According to the present invention in order to achieve the above object, there is provided a cosmetic composition containing as an active ingredient a mixed extract obtained through far-infrared extraction after drying and mixing honey, omija, goji berry and green tea in equal amounts, and the total weight of the cosmetic composition 0.001 to 20.0% by weight with respect to the content.

The far-infrared rays are to be treated with a wavelength of 2 to 20 μm at 80 to 130° C. for 30 to 180 minutes, and more preferably, to be treated with a wavelength of 2 to 20 μm at 90 to 120° C. for 60 to 120 minutes. In addition, extraction is performed by treatment at a temperature of 60 to 90° C. for 2 to 12 hours.

At this time, the far-infrared mixed extract is selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, and ethylene glycol. It is characterized in that the extraction is performed using any one or a mixture of two or more extraction solvents. It is a cosmetic composition having a skin moisturizing effect.

The cosmetic composition may be used for antioxidant, skin aging prevention, skin wrinkle improvement and skin elasticity improvement, skin whitening, skin damage suppression due to ultraviolet rays, skin irritation alleviation, or skin moisturizing.

The cosmetic composition is a sheet mask, water-soluble liquid, lotion, cream, gel, pack, essence, oil-in-water (O/W), water-in-oil (W/O) type and water-in-silicone (W/Si) type powder. It is a cosmetic composition having a skin whitening and moisturizing effect comprising honey, omija, goji berry and green tea far-infrared mixed extract as an active ingredient, characterized in that it has any one formulation selected from.

The cosmetic composition for whitening or moisturizing containing honey, omija, goji berry and green tea far-infrared mixed extract of the present invention as an active ingredient provides moisture retention effect by inhibiting tyrosinase activity, inhibiting melanin production, and enhancing skin moisture and skin barrier. It can be used as a cosmetic composition for whitening or moisturizing because it has no side effects such as skin allergies, troubles, and toxicity.

1 is a graph showing the cell proliferation effect for Examples 1 to 6.
Figure 2 is a graph showing the tyrosinase activity inhibitory effect of Examples 1 to 6.
Figure 3 is a graph showing the inhibition rate of melanin synthesis of Examples 1 to 6.
4 is a graph showing the skin moisturizing effect of Preparation Examples 1 to 6 and Comparative Examples 1 to 2;
5 is a graph showing the moisture loss rate of Preparation Examples 1 to 6 and Comparative Examples 1 to 2;

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown as examples of the embodiments. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

The present invention is characterized by containing 0.001 to 20.0% by weight of the mixed extract extracted by mixing honey, omija, goji berry and green tea in a dry weight ratio of 1: 1: 1: 1 and extracted by far-infrared extraction (F-IRE). It provides a cosmetic composition having a whitening and moisturizing effect.

The far-infrared radiation is a kind of electromagnetic wave and has a wavelength of 3.0 to 1,000 μm, and has a characteristic of evenly transferring heat to the center of a material by radiant energy that is transmitted stronger and faster than visible light by heating or non-heating methods. In addition, nutrient components and biological activity may be increased due to thermal decomposition, and may affect the enhancement of the activity of polyphenols or flavonoids exhibiting antioxidant activity.

The honey is one of the health foods obtained from nature and is rich in glucose and fructose protein. Honey is known to be a very effective wound treatment for patients with severe skin wounds or burns. In addition, Manuka honey is used not only as a raw material for pharmaceuticals but also as a raw material for various types of cosmetics. In Korea, honey is added to cosmetics and soaps to improve moisturizing power.

When the tyrosinase activity inhibition and melanin production inhibitory effects of a total of four honeys including chestnut honey and acacia honey were measured, it was confirmed that positive effects occurred in all honeys. In addition, honey is used in cosmetic formulations as a moisturizing ingredient, and it is used in various formulations such as sun products for its unique moisturizing and skin soothing functions.

The green tea refers to a material made to drink by picking young leaves of tea trees, and as it grows in high mountains, the quality is excellent. According to the processing method of tea leaves, it is divided into green tea, ohryong, cheolguaneum, yellow tea, black tea, puer tea, matcha tea, and hwacha. Depending on the shape of the tea leaves, it is divided into Jakseolcha (because the shape of the tea leaves resembles a sparrow's tongue) and Eungdo (because the shape of the leaves of tea resembles the nails of a hawk).

Green tea is made raw (baked tea) or steamed (steamed) before the fermentation process takes place. In the case of green tea, depending on the time of harvesting the leaves or the size of the tea leaves, Ujeon (first water tea, between early May), Sejak (Doomul tea, late May-June), medium crop (Semul tea, July-August), and Daejak ( End-water tea (late August), etc. Since it is made by directly picking tea leaves, there is little change in the composition and the content of vitamin C is higher than that of oolong and black tea.

Many studies have been conducted on the antioxidant activity of green tea. As one of them, as a result of a study on the antioxidant and tyrosinase inhibitory effects of a mixture of Eosinaceae, perilla leaf, and green tea plants, the antioxidant activity was more than 80% when mixed treatment, and in the case of whitening effect, perilla leaf and green tea extract showed a concentration-dependent whitening effect. was shown, and similarly to the mixture, it had an inhibitory effect on tyrosinage activity. Green tea has been shown to have whitening and antioxidant functions as well as moisturizing effects. It was confirmed that skin roughness and moisturizing ability were improved when the formulation containing 6% green tea extract was applied to 24 panelists for 30 days.

Goji berry is a woody plant belonging to the Solanaceae family, and it is a herbal medicine that grows or grows wild in Korea, China, Taiwan, Japan, and Europe. Goji berries, leaves, and roots are used for each part, and the Goji berries, leaves, roots, etc. include betaine, rutin, kukoamine A, beta-sitosterol, etc. It contains a lot of functional ingredients. In addition, vitamins A, B1, B2, C calcium, phosphorus, iron, zinc, and nicotinic acid are rich in nutrients. In terms of efficacy, there was a report on the effect of regulating the pigmentation of the skin, restoring the lipid composition change and the decrease in collagen synthesis, and in the study on the effect of collagen biosynthesis, the effect of Goji berry is the best. It has been reported that it is effective in inhibiting the activity of enzymes capable of inhibiting melanin production and degrading collagen.

As a result of measuring the melanin inhibitory activity using Goji berry extract, the whitening efficacy was confirmed to be 98.36, 82.34, and 81.86%, respectively, compared to the group treated with α-MSH alone at concentrations of 250, 500, and 750 μg/mL. This effect was also confirmed in other experimental results. Hwang et al. confirmed that the tyrosinase inhibitory activity was increased in a concentration-dependent manner when the skin whitening action of the ethanol extract of Angelica asiatica and Goji berry was confirmed.

Omija is named because it has five flavors (sour, salty, spicy, bitter, and sweet), and has been used as a tonic for thousands of years, and has been used as a drink to relax nerves and clear the head. Omija is rich in vitamin C, minerals such as calcium and iron, and contains lignans, which are plant estrogens, which have various antioxidant effects.

A study was conducted on the inhibition of melanogenesis and the whitening effect of Schisandra, which significantly inhibited melanogenesis and tyrosinase activity. In the study of Yan et al., the whitening efficacy of Schisandra powder was tested, and the inhibitory ability of B16 melanoma cells was confirmed.

Accordingly, the present inventors searched for and studied raw materials having both whitening and moisturizing effects, and as a result of research, the mixed extract extracted by irradiating honey, omija, goji berry, and green tea with far-infrared rays, as well as skin whitening and moisturizing effects, as well as active oxygen removal and cell protection The present invention was completed by finding that it has an excellent synergistic effect showing the effect.

In particular, far-infrared rays are irradiated to honey, Schisandra, Goji berry, and green tea that contain natural organic substances such as glucose, fructose, protein, carbohydrate, mineral, organic acid, and enzyme in order to provide skin whitening and moisturizing effects. The mixed extract extracted afterward was used as an existing active ingredient.

According to a preferred embodiment of the present invention, the cosmetic composition having a whitening and moisturizing effect containing honey, omija, goji berry and green tea far-infrared mixed extract according to the present invention as an active ingredient contains honey and dried omija, goji berry and green tea by dry weight. Contrast It would be appropriate to contain 0.001 to 20% by weight of the mixed extract extracted after mixing in a weight ratio of 1: 1: 1: 1 and irradiated with far-infrared rays, based on the total weight of the cosmetic composition.

When the content is less than the above content, the effect on skin whitening and moisturizing is difficult to appear properly, and even if used in excess of the content, the effect does not increase any more.

The cosmetic composition for skin whitening and moisturizing effect according to the present invention is a combination based on scientific basic data on skin, and when honey, omija, goji berry, and green tea far-infrared mixed extract are used as active ingredients, it is substantially long-term in terms of skin whitening and moisturizing. effectiveness can be realized.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the honey, omija, goji berry and green tea far-infrared mixed extract according to the present invention, for example, antioxidants, stabilizers, solubilizers, vitamins , conventional adjuvants such as pigments, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, water-soluble liquid, lotion, cream, gel, pack, essence, oil-in-water (O/W), water-in-oil ( It may be formulated as a powder of W/O) type or water-in-silicone (W/Si) type, but is not limited thereto.

The composition of each formulation may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

The efficacy of the cosmetic composition containing honey, omija, goji berry and green tea far-infrared mixed extract having skin whitening and moisturizing effects according to the present invention as an active ingredient depends on the individual's skin condition and condition, external environmental conditions, taste, or common usage method. The number and amount of use can be adjusted accordingly.

In addition, the use efficacy of the cosmetic composition according to an embodiment of the present invention can be appropriately adjusted according to the individual's skin condition and condition, and external environmental conditions.

Hereinafter, to help the understanding of the present invention will be described in detail by presenting examples and experimental examples. However, the following examples and experimental examples are only provided for easier understanding of the present invention, and should not be construed as limiting the technical level and scope of the present invention thereby. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of honey extract

1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of honey, and the mixture was extracted for 2 to 12 hours at 60 to 90 ° C. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 2> Schisandra extract

1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dried omija, and the mixture was extracted at 60 to 90° C. for 2 to 12 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 3> Goji berry extract

1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dried goji berries, and extracted for 2 to 12 hours at 60 to 90 ° C. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 4> Green tea extract

1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dried green tea, and the mixture was extracted at 60 ~ 90 °C for 2 ~ 12 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 5> Mixed extract

1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of the raw materials of Examples 1 to 4 mixed in a ratio of 1:1 to 1:1 by weight, and the mixture was extracted at 60 to 90° C. for 2 to 12 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 6> Far-infrared treatment mixed extract

100 g of the raw materials of Examples 1 to 4 were mixed in a ratio of 1: 1: 1: 1 by weight, treated with far infrared rays having a wavelength of 2 to 20 μm at a temperature of 90 to 120 ° C. for 60 to 120 minutes, and then 70% ethyl alcohol 1 kg of aqueous solution was added, and extraction was performed at 60 to 90° C. for 2 to 12 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

As in the above-described examples, honey extract, omija extract, goji berry extract, green tea extract, honey, omija, wolfberry and green tea mixed extract, far-infrared-treated honey, omija, wolfberry and green tea mixed extract were prepared as shown in Table 1, respectively.

Ingredient name Sample name Preparation Example 1 Preparation Example 2 Preparation 3 Preparation 4 Production Example 5 Preparation 6 Comparative Example 1 Comparative Example 2




A honey extract One Schisandra extract One Goji berry extract One green tea extract One Mixed extracts (honey, omija, goji berry, green tea) One Far-infrared treatment mixed extract (honey, omija, goji berry, green tea) One glycerin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Butylene Glycol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 hexanediol 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 EDTA-2NA 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100



B Methylglucose sesquistearate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl Stearate 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 beeswax 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Caprylic/Capric Triglycerides 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 shea butter 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 dimethicone 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Tocopheryl Acetate 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 fragrance, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

< test example 1 > Cell viability test: MTT assay

1) Experimental method

For the samples prepared in Examples 1 to 6, the effect on cell proliferation was confirmed. In order to confirm cell proliferation and toxicity, the experiment was performed as follows.

Cells being cultured 3T3 (fibroblast cell line) were dispensed in a 96-well microplate at 5,000 cells/well and incubated in a thermostat for 30 minutes, and the samples were incubated in a thermostat for each concentration of 0.01, 0.05, 0.1, 0.5 (%, W/ V)) and cultured for 72 hours.

After 72 hours of incubation, Thiazolyl Blue was administered and further incubated for 4 hours. After discarding all of the culture medium, a reaction stop solution was added to each well of the microplate, stirred for 5 minutes, and absorbance was measured at 570 nm. The control group was co-cultured under optimal conditions for cell growth by administering 10% Fetal Bovine Serum (FBS) medium as much as the sample injection amount, and the cell proliferation of the control group was set to 100% and the cell proliferation rate of the sample-injected experimental group was calculated. .

The cell proliferation effect was calculated by Equation 1 below, and the results are shown in Table 2 below.

Sample name Sample treatment concentration (㎍/㎖) 0 50 100 200 500 1,000 Example 1 100 101.7 103.6 105.9 109.8 111.2 Example 2 100 100.3 101.7 101.8 102.3 103.7 Example 3 100 101.5 102.5 103.8 106.6 108.1 Example 4 100 101.6 102.8 104.2 107.3 110.5 Example 5 100 102.2 105.3 108.7 114.6 116.2 Example 6 100 104.6 108.6 115.5 123.9 127.2

As shown in Table 2, cell viability was confirmed for the cosmetic compositions prepared according to Examples 1 to 6.

As a result, it was confirmed that all of Examples 1 to 6 had no cytotoxicity, and in particular, it could be confirmed that the far-infrared irradiation had an excellent effect on cell promotion through Example 6.

< test example 2 > Inhibition of tyrosinase activity

1) Experimental method

Tyrosinase (Tyrosinase) activity inhibition experiment was an experiment to confirm the melanin activity inhibitory effect of the substance, and was measured by applying a method such as Yagi, which measures DOPA chrome by a colorimetric method.

In this experiment, a reaction solution was prepared by adding 40 μl of 1.5 mM L-Tyrosine solution to 120 μl of 0.1M phosphate buffer pH 6.5 in a 96-well plate, and sample solution 10 was added to the reaction solution. After putting in μl and reacting for 5 minutes, 10 μl of mushroom tyrosinase (100 unit/ml) was added and the enzymatic reaction was performed at 37° C. for 10 minutes. After 10 minutes, dopachrome generated in the reaction solution was measured at 490 nm using a microplate counter, and vitamin C (Ascorbic acid) was used at the same concentration as a positive control as a comparison target for the test results.

The tyrosinase activity inhibition rate was calculated by Equation 2 below, and the results are shown in Table 3 below.

Sample name Sample treatment concentration (㎍/㎖) 50 100 200 500 1,000 Example 1 16.3 22.6 36.3 44.8 53.7 Example 2 15.0 19.4 34.7 42.6 51.7 Example 3 15.5 20.9 35.1 43.5 51.9 Example 4 16.1 21.3 35.8 44.3 52.5 Example 5 22.7 28.5 44.6 50.9 57.4 Example 6 35.7 55.9 66.5 69.2 72.1 Ascorbic acid 41.4 58.6 66.8 68.9 70.2

In all of Examples 1 to 6, it was confirmed that the inhibition rate gradually increased in a concentration-dependent manner, and when the raw material was mixed as in Example 5, it was confirmed that the effect increased than in Examples 1 to 4.

In particular, when irradiating far-infrared rays to the raw material mixed as in Example 6, it was confirmed that the effect was further increased as shown in Table 3.

Thus, it can be seen that the mixing of honey, omija, goji berry, and green tea raw materials and the conditions of far-infrared irradiation treatment show a synergistic effect that maximizes the efficacy, and is effective in inhibiting tyrosinase enzyme activity.

<Test Example 3> Inhibition of melanin synthesis

1) Experimental method

B16F10 mouse melanoma cells were aliquoted in a 6-well plate at a ^{concentration of 3.5 × 10 5} cells/well and cultured for 24 hours, and then each well was treated with α-MSH (melanocyte stimulating hormone) and the samples prepared for each concentration. After culturing for 48 hours, the medium was removed by washing twice with phosphate buffer saline (PBS), and the adhered cells were transferred to an EP tube and centrifuged to obtain a cultured cell pellet. The shape of the precipitated B16F10 melanoma cells was photographed, and after dissolving melanin in DMSO-added 1N NaOH solution at 60° C. for 1 hour, 200 μl of each was dispensed into a 96-well plate and absorbance was measured at 405 nm.

The inhibition of melanin synthesis was calculated by Equation 3 below, and the results are shown in Table 4 below.

Sample name Sample treatment concentration (㎍/㎖) 50 100 200 500 1,000 Example 1 4.1 5.4 8.5 12.9 16.8 Example 2 3.2 4.0 5.7 7.8 10.9 Example 3 3.5 4.3 6.8 10.7 14.5 Example 4 3.6 4.6 7.0 11.0 15.3 Example 5 6.8 10.5 14.8 16.7 20.6 Example 6 10.3 16.4 24.1 31.5 38.5 Arbutin 11.6 20.1 29.2 36.8 41.7

In Examples 1 to 6, it was confirmed that the inhibition rate of melanin synthesis was increased in a concentration-dependent manner, and Example 6 showed an inhibitory rate most similar to that of arbutin, a positive control, as compared with Examples 1 to 5. Confirmed.

In particular, when irradiating far-infrared rays to the mixed raw material as in Example 6, it can be confirmed that the inhibition of melanin synthesis increases as the activity of inhibiting melanin synthesis increases.

< test example 4 > Skin moisturizing effect

1) Experimental method

Cosmetics prepared in Preparation Examples and Comparative Examples of Table 1 were applied to 30 test subjects (male and female aged 20-50 years) twice a day for 4 weeks on both sides of the face and in the first half of Preparation Examples 1 to 6 and Comparative Examples 1 and 2, respectively, the skin moisture improvement state before, after 2 weeks, and after 4 weeks of application was measured using a skin moisture meter Corneometer CM 820 (Courage + Khazaka, Germany), The unit is expressed as an arbitrary unit (Arbitrary Unit, AU) given by the device, and the higher the measured value, the higher the moisture content on the skin surface. The experimental results on skin moisture improvement are shown in Table 5 below.

2) Experiment result

Sample name Skin moisturizing effect (AU) before application after 2 weeks after 4 weeks Comparative Example 1 54.7 56.3 58.5 Comparative Example 2 54.5 54.9 55.7 Preparation Example 1 54.7 59.3 64.6 Preparation Example 2 55.1 60.7 66.8 Preparation 3 54.8 58.7 65.1 Preparation 4 54.9 59.9 66.3 Production Example 5 55.4 61.6 71.6 Preparation 6 54.8 72.5 83.4

As shown in Table 5 above, the moisturizing effect was measured for the cosmetic compositions prepared according to Preparation Examples 1 to 6 and Comparative Examples 1 to 2, and as a result, the moisturizing effect was higher in Preparation Examples 1 to 6 than Comparative Examples 1 to 2 could check

In particular, as a result of comparing Preparation Example 5 and Preparation Examples 1 to 4, it was confirmed that the skin moisturizing improvement effect was increased when the raw material was mixed, and when irradiated with far infrared rays to the mixed raw material as in Preparation Example 6, the moisturizing effect was more increase could be observed.

< test example 5 > transdermal water loss evaluation

1) Experimental method

For each of the cosmetic compositions of Preparation Examples 1 to 6 and Comparative Examples 1 to 2 in Table 1, 30 subjects (male and female aged 20-50 years) set the upper arm as the measurement site, Measurements were made after marking the test site with a size of 2 cm ^{2 .} After applying the cosmetic composition to the test site, it was rubbed well, and the amount of transdermal moisture loss was measured using TEWAMETER TM210 (C+K eletronic GmhH. Germany). Short-term moisturizing effect was evaluated by measuring the moisture loss rate (%) 30 minutes after application, 4 days after application, and 8 days after application, based on the water retention amount of 0 hours before application. During the measurement, the amount of transdermal moisture loss was measured three times consecutively while keeping the probe vertically for 60 seconds. Measurement was carried out in the same temperature and humidity room, and the higher the value, the greater the loss of moisture. The results for the amount of transdermal water loss are shown in Table 6 below.

2) Experiment result

Sample name Moisture loss rate (%) 30 minutes after application 1 day after application 4 days after application 8 days after application Comparative Example 1 34.76 33.48 32.71 32.03 Comparative Example 2 38.33 40.02 39.47 38.96 Preparation Example 1 29.65 27.66 25.47 21.68 Preparation Example 2 31.74 29.11 26.26 23.35 Preparation 3 31.21 28.39 25.92 22.51 Preparation 4 30.94 28.09 25.54 22.24 Production Example 5 27.73 24.52 22.49 20.59 Preparation 6 24.71 18.44 15.32 12.67

As shown in Table 6, as a result of comparing the amount of transdermal water loss in Preparation Examples 1 to 6 and Comparative Examples 1 to 2 for 8 days, it was confirmed that the improvement in water loss was the highest in Preparation Example 6 over time. In particular, even by comparing the individual extracts of Preparation Examples 1 to 4 and Preparation Example 5, which is a mixed extract, better moisture loss could be improved when the raw material was mixed. It was confirmed that the effect was more increased than that of the mixed extract.

< Formulation Example 1> Preparation of lotion containing honey, omija, goji berry and green tea far-infrared mixed extract according to the present invention

Example 6 Prescription examples of lotion (skin lotion) among cosmetics containing honey, omija, goji berry and green tea far-infrared mixed extract are shown in Table 7.

number Raw material content (wt%) One Example 6
Honey, omija, goji berry and green tea far-infrared mixed extract 1.0 2 glycerin 3.0 3 butylene glycol 2.0 4 propylene glycol 2.0 5 Polyoxyethylene hydrogenated castor oil 1.0 6 ethanol 10.0 7 triethanolamine 0.1 8 antiseptic a very small amount 9 pigment a very small amount 10 Spices a very small amount 11 Purified water remaining amount

<Production method>

After putting No. 2, 3, 4, and 8 into No. 12 in this order and stirring to dissolve, No. 5 is heated to about 60℃ to dissolve, No. 10 is put in to dissolve, and then No. 11 is added. Finally, add Nos. 6, 7, and 9, but add No. 1, stir thoroughly, and then ripen.

< Formulation Example 2> Preparation of nutritional lotion containing honey, omija, goji berry and green tea far-infrared mixed extract according to the present invention

Example 6 Prescription examples of nutritional lotion among cosmetics containing honey, omija, goji berry and green tea far-infrared mixed extract are shown in Table 8.

number Raw material content (wt%) One Example 6
Honey, omija, goji berry and green tea far-infrared mixed extract 1.0 2 beeswax 1.0 3 Polysorbate 60 1.5 4 Sorbitan Sesquiolate 0.5 5 liquid paraffin 10.0 6 Sorbitan Stearate 1.0 7 lipophilic monostearate glycerin 0.5 8 stearic acid 1.5 9 Glyceryl Stearate / PEG-400 Stearate 1.0 10 propylene glycol 3.0 11 carboxy polymer 0.1 12 triethanolamine 0.2 13 antiseptic a very small amount 14 pigment a very small amount 15 Spices a very small amount 16 Purified water remaining amount

<Production method>

10, 11, 13, and 16 are mixed and stirred at 80 ~ 85 ℃, heated to 80 ~ 85 ℃, put into the production unit, the emulsifier works, 2, 3, 4, 5, 6, 7, 8, 9, 12 at 80 ~ 85 ℃ Emulsify after melting by heating. After the emulsification is finished, while stirring using a stirrer, cool to 50°C, add No. 16, cool to 45°C, add Nos. 14 and 15, put No. 1 at 35°C, cool to 25°C, and then mature.

< Formulation Example 3> Preparation of nutritional cream containing honey, omija, goji berry and green tea far-infrared mixed extract according to the present invention

Example 6 A prescription example of a nourishing cream among cosmetics containing honey, omija, goji berry and green tea far-infrared mixed extract is shown in Table 9.

number Raw material content (wt%) One Example 6
Honey, omija, goji berry and green tea far-infrared mixed extract 1.0 2 stearic acid 2.0 3 cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 8 wax 1.0 9 liquid paraffin 4.0 10 squalane 4.0 11 Caprylic/Capric Triglycerides 4.0 12 Carboxyvinyl Polymer 0.3 13 butylene glycol 5.0 14 glycerin 3.0 15 triethanolamine 0.1 16 Purified water remaining amount

<Production method>

12, 13, 14, and 16 are mixed and stirred, heated to 80 ~ 85 ℃, put into the manufacturing unit, and then the emulsifier works, and 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 are heated to 80 ~ After dissolution by heating to 85°C, 15 is added and stirred to put into the manufacturing unit and emulsified. When the emulsification is finished, it is cooled to 35°C while stirring using a stirrer, and then cooled to 25°C by adding No. 1 and then aged.

< Formulation Example 4> Preparation of essence containing honey, omija, goji berry and green tea far-infrared mixed extract according to the present invention

Example 6 Table 10 shows the formulation examples of essence in cosmetics containing honey, omija, goji berry and green tea far-infrared mixed extract.

number Raw material content (wt%) One Example 6
Honey, omija, goji berry and green tea far-infrared mixed extract 1.0 2 sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 dicetyl phosphate 0.4 8 concentrated glycerin 5.0 9 macadamia oil 15.0 10 Carboxyvinyl Polymer 0.2 11 xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water remaining amount

<Production method>

2, 3, 4, 5 and 6 are homogenized at a constant temperature and are referred to as nonionic amphiphilic lipids. Mix 7, 8, and 14 with the nonionic amphiphilic lipids, but add No. 1 to mix, homogenize at a constant temperature, pass through a microfluidizer, and then slowly add 9 at a constant temperature to homogenize and then re-micro Pass it through the fluidizer again, and add 10, 11, 12, and 13 to disperse, stabilize and mature.

Although the present invention has been described with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be construed by the following claims, and all technical ideas within the equivalent range by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (4)

Honey, omija, wolfberry and green tea are mixed in a weight ratio of 1: 1: 1: 1, and a mixed extract extracted by far-infrared extraction (F-IRE) is an active ingredient. Honey, omija, wolfberry and green tea far-infrared blend A cosmetic composition containing an extract as an active ingredient and having a whitening and moisturizing effect.
According to claim 1,
The cosmetic composition,
A cosmetic composition having a whitening and moisturizing effect comprising honey, omija, goji berry and green tea far-infrared mixed extract as an active ingredient, characterized in that it contains 0.001 to 20.0% by weight of the mixed extract based on the total weight.
According to claim 1,
The mixed extract is prepared by extraction with at least one extraction solvent selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, butylene glycol Honey, Schisandra, A cosmetic composition having a whitening and moisturizing effect containing a mixed extract of Goji berry and green tea far-infrared as an active ingredient.
4. The method according to any one of claims 1 to 3,
The cosmetic composition is composed of sheet mask, water-soluble liquid, lotion, cream, gel, pack, essence, oil-in-water (O/W), water-in-oil (W/O) type and water-in-silicone (W/Si) type powder. A cosmetic composition having a whitening and moisturizing effect comprising honey, omija, goji berry, and green tea far-infrared mixed extract as an active ingredient, characterized in that it has any one formulation selected from the group.

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