KR20100028256A - Skin protective cosmetic composition comprising extracts from complex medical herbs - Google Patents

Abstract

PURPOSE: A cosmetic composition for skin protection containing medicinal herb is provided to protect skin from toxic oxygen and to ensure elasticity and anti-aging. CONSTITUTION: A cosmetic composition for skin protection contains medicinal herb extract having Angelica gigas, Scrophularia buergeriana, Panax ginseng, Astragalus membranaceus, Polygonatum odoratum, and Liliope platyphylla. The cosmetic composition for skin protection contains cosmetically acceptable carrier. The cosmetic composition contains 15-25 weight parts of Agelica gigas Nakai or Scrophularia buergerizna, 5-20 weight parts of Panax ginseng, 10-25 weight parts of Astragalus membranaceus, 25-35 weight parts of polybonatum, 25-30 weight parts of Liriope platyphylla.

Description

Skin Protective Cosmetic Composition Comprising Extracts from Complex Medical Herbs}

The present invention relates to a cosmetic composition for skin protection comprising a complex herbal extract, more specifically, a cosmetic composition for skin protection comprising at least one selected from tangui and Hyunsam, ginseng, Astragalus, Hwangjeong and Macmundong as an active ingredient It is about.

Skin is the largest organ of body tissues, and it is always in contact with the outside and acts as a protective shield to protect the living body from various external stimuli or dry environment. It also plays an important role in preventing the loss of moisture in the body and protecting it from harmful ultraviolet rays from sunlight.

In living cells, various free radicals occur during ultraviolet irradiation or during normal metabolic processes. These free radicals are mostly harmful free radicals. These harmful free radicals are mostly destroyed by self-protective mechanisms in vivo, but some of the free radicals attack unsaturated fatty acids to form lipid peroxide and damage biopolymers such as proteins. As a result, the cells are damaged and their vitality is reduced.

In addition, continuous exposure to oxidative stress from harmful environments such as air pollution, UV exposure, stress, or disease increases the free radicals in the body and destroys the dermal connective tissue collagen, elastin, hyaluronic acid, and soothes certain parts of the skin. Symptoms (wrinkles), and can oxidize the lipid portion of the cell membrane can cause cell destruction, leading to diseases such as dermatitis, acne or skin cancer. In addition, free radicals are involved in the melanin formation process, causing blemishes, freckles and wrinkles.

Therefore, anti-oxidants such as substances capable of scavenging free radicals and lipid peroxidation inhibitors are expected as therapeutic agents for preventing various diseases caused by free radicals and inhibitors for aging.

Conventionally, ascorbic acid, di-alpha-tocopherol or beta-carotene are active oxygen scavenging substances, which are used in cosmetics and medicines to prevent wrinkles and other skin diseases. However, they are not only expensive, but also have poor chemical stability during formulation, making it difficult to expect a substantial effect. For this reason, the development of safer and more active oxygen scavenging effect has emerged as an important task not only in the pharmaceutical and food sectors but also in the cosmetics industry.

In order to enhance skin moisturizing and antioxidant activity, cosmetics containing mainly chemically prepared ingredients have been used. However, in the case of the cosmetics there is a problem that the skin is damaged by the side effect occurs when used for a long time. In order to prevent the above problems, there have been attempts to manufacture cosmetics based on extracts extracted from various plants, fruits, and herbal medicines. Such cosmetics have the advantage of not only reducing the side effects of the skin but also being easily absorbed by the skin to provide a fresh texture and moisturizing and whitening effects. In particular, recently, there have been attempts to manufacture cosmetics having antioxidant effects to prevent skin aging as well as whitening and moisturizing effects. For example, the following techniques are disclosed in the art.

Korean Patent Registration No. 10-0820009 discloses an herbal composition for antioxidant and skin soothing containing persimmon leaf, licorice, baeknyeoncho and baekryepi and its manufacturing method.

Republic of Korea Patent No. 10-0846738 No. Pokryeong ( Poria cocos wolf ) extract and a manufacturing method and a cosmetic composition containing the Fukryeong extract containing 0.0001 to 30% by weight of Bokryeong extract and a cosmetic having excellent antioxidant and skin cell protection effect The composition is disclosed.

As described above, a cosmetic composition using various herbal extracts as an active ingredient is disclosed, but in most cases, there is a problem that the antioxidant activity is insufficient or such effects are not clearly demonstrated.

Thus, the present inventors are looking for a herbal medicine that can improve the moisturizing and antioxidant function of the skin, at least one selected from Angelica and Hyunsam, and a cosmetic composition for skin protection comprising a herbal medicine extract consisting of ginseng, Astragalus, Hwangjeong and Macmundong as an active ingredient As a result of applying to the skin confirmed that the skin moisturizing effect and antioxidant effect is excellent and came to complete the present invention.

The present invention relates to a cosmetic composition for skin protection comprising at least one selected from Angelica tang and Hyunsam, and ginseng, Astragalus, Yellow Jung and Macmundong extract as an active ingredient.

First, it will be described in detail with respect to the oriental medicine medicinal herbs constituent extracts, tangsam, ginseng, ginseng, Astragalus, Hwangjeong and Mcmundong.

Angelica gigas is a 2-3-year-old herb that uses roots and has a single shot of blood, colloquial blood, and landscaping. It is used for diseases such as physical weakness, joint pain, headache, dizziness, abdominal pain, constipation, and bruises.It is used for decursin, decursinol, nodakenin, and alpha-pinene ( α-pinene, limonen, elemol and the like have been reported.

' Scrophularia buergeriana ' is a perennial plant with Korean ginseng, grows well in the mountains, uses roots, and is used to treat pharyngitis, boils and lymphadenitis with antipyretics in Chinese medicine.

Panax ginseng is a perennial herb of the elderaceae family. It is known to elevate the yang of the five intestines, ie, the liver, heart, lungs, kidneys and spleen, to stabilize the mind and brighten the eyes, and to treat various cancers and diabetes. It is also used. It has been reported to contain various types of saponins, polyacetylene compounds, polyphenol compounds and acidic polysaccharides.

' Astragalus membranaceus ' is a perennial herb belonging to the legume, and its roots are used, and the roots of Astragalus are reported to contain astragalaloside, a kind of saponin, and hepatic, diuretic, hypotensive, and antibacterial. It is known to have pharmacological effects such as hepatoprotective action.

' Polygonatum odoratum ' is a perennial herbaceous herbaceous herb that grows around the slopes of wild wasteland, in the surrounding areas of shrubs and shrubs, and has antibacterial, antifungal and blood pressure-lowering effects. Used for intestinal life, rhizome contains mucus, starch and sugar, and rhizome contains azetidine-2-carboxylic acid, aspartic acid, homeserine, It contains diaminobutylic acid, digitalis glicoside and various andandraquinone compounds, and leaves contain vitexin and xyloside. It is reported.

'Liliope platyphylla' is a perennial herb of the family Liliaceae, and is effective in relieving limbs, neuralgia and rheumatism, which is useful for the elderly, recovery patients, and weak constitutions. In addition, it is effective in relieving cough, cooling phlegm and lowering heat, and is used for pulmonary tuberculosis, chronic bronchitis, and chronic sore throat. It has been reported to contain steroidal saponins such as β-sitosterol and stigmasterol.

According to an aspect of the present invention, the present invention provides a cosmetic composition for skin protection comprising at least one selected from Angelica tang and Hyunsam, and ginseng, Astragalus, Hwangjeong and Macmundong extract as an active ingredient.

According to another aspect of the present invention, the cosmetic composition for skin protection is 15 to 25 parts by weight, 5 to 20 parts by weight, ginseng 5 to 20 parts by weight, 10 to 25 parts by weight, yellow gold 25 to 35 parts by weight based on the total weight of the herbal extracts It may be characterized by containing 25 to 30 parts by weight of the portion and pulmonary dong.

The present invention may also be characterized in that the herbal extracts are contained in an amount of 0.05% to 30% by weight based on the total weight of the cosmetic composition for protecting the skin.

Included in the composition of the present invention Herbal extracts may be prepared according to conventional methods known in the art.

Included in the composition of the present invention The solvent for preparing the herbal extracts is (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol with water, (d) acetone, (e) ethyl acetate, (f) chloroform and (g) 1,3-butylene glycol. Preferably, water is used as the extraction solvent. In addition, the extraction temperature for producing the herbal medicine extract of the present invention is preferably 50 to 85 ℃, more preferably about 70 ℃.

Referring to the embodiment of the present invention, the preparation method of the herbal medicine extract is as follows. After distilled water is added to at least one selected from Angelica vulgaris and Hyunsam, and ginseng, Astragalus, Hwangjeong and McMoon-Dong, and extracted by heating at 85 ° C. for about 150 minutes, the extract is filtered under reduced pressure and the filtrate is concentrated.

On the other hand, the herbal extracts included in the composition of the present invention includes not only the extract by the above-described extraction method, but also extracts that have undergone conventional processes. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extracts included in the composition of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of the herbal extract is 0.05 to 30% by weight, preferably 0.1 to 10% by weight based on the total weight of the total composition. If the content of the herbal extract is less than 0.05% by weight can not expect a clear skin protection effect .

The cosmetic composition for skin protection of the present invention includes components commonly used in cosmetic compositions in addition to the natural herbal extract as an active ingredient, and includes, for example, conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. Include.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powders It may be formulated as a foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may be aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Cosmetic composition for skin protection comprising at least one selected from Angelica and Hyunsam according to the present invention, ginseng, Astragalus, Hwangjeong and Macmundong as an active ingredient has excellent skin moisturizing effect and antioxidant effect to prevent skin from harmful free radicals By protecting, skin aging can be prevented.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1. Preparation of Herbal Extracts

The scientific names and herbal names of the herbal medicines used in the present invention are shown in Table 1.

Table 1.

Korean name Scientific name Herbal medicine Donkey Angelica gigas Angelica Gigantis Radix Ginseng Panax ginseng Gingeng Radix Astragalus Astragalus membranaceus Astragali radix Imperial Polygonatum Odoratum Polygonati Rhizoma Macmundong Liriope platyphylla Liriopis Tuber figwort Scrophularia buergeriana Scrophulariae Radix

At least one selected from tangui and hyeonsam, as shown in Table 2 below to prepare a herbal extracts included as an active ingredient in the composition according to the invention, 90 g of a sample consisting of ginseng, Astragalus, hwangjeong and Mcmundong were put together with 1000 mL of primary distilled water in an electric bath and extracted by heating at 85 ° C. for 150 minutes. The extract was filtered under reduced pressure with a filter paper and the filtrate was concentrated using a rotary vacuum evaporator (Eyela, Japan). This concentrate was dried using a lyophilizer and used as a sample.

Table 2.

Example 2. Cytotoxicity Test of Herb Extract Sample

(1) cell culture

The cells used in the experiment are human skin fibroblasts. Human dermal fibroblasts were treated with Delbecco's Modified Eagle Medium (DMEM, WelGENE, supplemented with 10% Fetal Bovine serum (FBS, Gibco, CA), 100 U / ml penicillin and 100 μg / ml streptomycin at 37 ° C. and 5% CO ₂ ). Korea) cultured in the medium. Cells were grown in 75 cm 2 flasks (Falcon, USA) and washed at 3 days of culture with phosphate buffered saline (PBS, Gibco, CA) solution. The cells were washed with 1 mL of 0.25% tyrosin-EDTA solution per 50 ml flask. After treatment for 1 minute at room temperature, the tyrosin solution was discarded and stored at 37 ° C. for 5 minutes to detach and passage cells. The detached cells were suspended in 10 ml of DMEM medium added with 10% FBS, and then transferred to a new culture vessel and cultured in a CO ₂ incubator (37 ° C., 5% CO ₂ ) at a ratio of 1: 2.

(2) cytotoxicity test

1) test principle

In order to test the cell proliferation ability of the test substance (symbiose I, II sample) using human skin fibroblast cells, the test substance was treated and the cells were cultured to determine the cell survival rate. The MMT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) assay utilizes mitochondrial dehydrogenase activity, which is active only in living cells, to form a pigment product called formazan. By measuring the degree of cytotoxicity.

2) Sample Preparation

The herbal extract samples prepared above were diluted with DMSO (dimethyl sulfoxide, Duchefa biochemicals, Netherlands) and refrigerated after dispensing. The MMT reagent was dissolved in PBS (pH 7.4) at a concentration of 1 mg / ml, filtered through a 0.2um syringe filter, and then aliquoted and stored in a refrigerator.

3) test method

In order to confirm whether the herbal extract prepared in Example 1 can show an improvement effect without skin irritation even after long-term use, it was tested for cytotoxicity against human skin fibroblasts. Cultured human dermal fibroblasts were put into 100 ml of 2 × 10 ⁴ cells / well in 96 well plate (Nunc, NY) at 37 ℃, 5% CO ₂ After incubation for 24 hours in the incubator, the cell adhesion was confirmed, then changed to serum-free media and each test material (Simbiose I, II samples) treated in each concentration of 1ppm to 100ppm as shown in Table 3. After incubation for 72 hours. 4 hours before the end of the incubation period, the MMT (Sigma, MI) solution dissolved in PBS at a concentration of 1 mg / mL was added in an amount of one-tenth of the total medium for each well. Shade and incubated at the same conditions for 4 remaining hours. After removing all of the culture solution, 100 µl of dimethyl sulfoxide (DMSO, Duchefa biochemicals, Netherlands) was treated in each well to dissolve intracellular formazan. Absorbance was measured at 540 nm using an ELISA reader (Powerwave X, Bio-tec INC, VT). Cell viability was calculated by the following formula.

(AC-absorbance of control, AT-absorbance of tested extract solution)

Table 3.

Treated substance (concentration) 1 time Episode 2 Average S.D % AV % SD Negative control 0.609 0.585 0.597 0.017 100 3 Symbioz I (1 ppm) 0.643 0.624 0.636 0.018 107 3 Symbioz I (10 ppm) 0.615 0.623 0.619 0.006 104 One Symbioz I (50 ppm) 0.621 0.639 0.630 0.013 106 2 Symbioz I (100 ppm) 0.704 0.633 0.668 0.05 112 8 Symbiose II (1 ppm) 0.556 0.566 0.561 0.007 94 One Symbiose II (10 ppm) 0.565 0.513 0.539 0.037 90 6 Symbioz II (50 ppm) 0.521 0.502 0.511 0.013 86 2 Symbioz II (100 ppm) 0.616 0.557 0.586 0.042 98 7

As a result, there was a statistically significant increase in the number of cells in the group treated with the Simobis I sample compared to the negative control group that did not process anything, but the statistically significant value was not significant. No cell number change was observed. Therefore, after 72 hours of incubation of human skin fibroblasts in a medium containing a test substance (Symbiose I, II samples), there was no decrease in specific cell viability, and as a result, the herbal medicine included as an active ingredient in the composition according to the present invention. The extract was found to be cytotoxic to human skin fibroblasts.

Example 3. Antioxidant Test of Herbal Extracts

(1) test principle

DPPH (1,1-diphenyl-2-picrylhydrazil) initiates oxidation with a purple radical. When the sample to be tested in the DPPH solution was treated together, the scavenging ability of the sample was directly confirmed through color change.

(2) test method

In order to investigate the antioxidant activity of the herbal extract prepared in Example 1 as shown in Table 3 below to see how much the amount of free radicals produced by mixing DPPH (Sigma, MI) solution and herbal extracts by concentration Confirmed. After dissolving the herbal extract sample prepared in Example 1 in distilled water or a solvent to make a liquid phase sample of the final concentration of 0.01% to 1%, put 20μL into 96well (Nunc, NY) and mix 180μL of 100uM DPPH solution at room temperature After incubation for 30 minutes, the amount of DPPH remaining was measured for absorbance at 540 nm using an ELISA reader (Powerwave X, Bio-tec INC, VT). The degree of antioxidant power was based on the negative control group consisting of only DPPH, distilled water or solvent without adding a sample. In comparison, the lower the absorbance and the lower the converted value, the better the free radical scavenging ability.

Table 4.

* O.D: absorbance value (O.D. value is the value corrected by subtracting the sample value without DPPH from the O.D value of the sample reacted with DPPH)

* Converted value: The negative control set to 100 and converted into percentage

As a result, as shown in Table 4 above, when 0.1% of the herbal medicine extract symbiose I according to the present invention was treated as compared to 100% of the negative control group, the converted value was lowered to 64 ± 3%, and 1% treatment. It was found that the time was lowered to 60 ± 11%. In addition, it was found that the converted value was reduced to 77 ± 3% for 0.1% treatment and 15 ± 2% for 1% treatment. Therefore, it was determined that both symbiose I and II had antioxidant power through DPPH free radical scavenging experiment.

1 is a graph showing the effect of a test substance (symbios I, II samples) on the proliferation of human skin fibroblasts.

Figure 2 is a graph showing the effect of the test substance (Symbios I, II samples) on DPPH free radical scavenging.

Claims (4)

At least one selected from Dong-Gi and Hyunsam, Cosmetic composition for skin protection comprising a herbal extract consisting of ginseng, Astragalus, Hwangjeong and Macmundong as active ingredients. The method of claim 1, The skin protective cosmetic composition is a skin protective cosmetic composition, characterized in that it contains a cosmetically acceptable carrier. The method of claim 1, The cosmetic composition for skin protection contains 15 to 25 parts by weight of Angelica or Hyunsam, 5 to 20 parts by weight, 10 to 25 parts by weight, yellow to 25 parts by weight, 25 to 35 parts by weight, and 25 to 30 parts by weight of Macmundong based on the total weight of the herbal medicine extract Cosmetic composition for skin protection, characterized in that. The method of claim 1, The cosmetic composition for skin protection is a cosmetic composition for skin protection, characterized in that the herbal extract is contained in an amount of 0.05 to 30% by weight based on the total weight of the composition.

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