KR102327946B1 - Cosmetic omposition including hydrolysate of paeonia lactiflora extracts for anti-oxidation, anti-inflammatory or anti-wrinkle, and manufacturing method thereof - Google Patents

Abstract

The present invention provides a cosmetic composition comprising hydrolysate prepared by acidolysis of a Paeonia lactiflora flower extract as an active ingredient so that an excellent anti-oxidation, anti-inflammation, or anti-wrinkle effect can be implemented; and a method for manufacturing the same.

Description

COSMETIC OMPOSITION INCLUDING HYDROLYSATE OF PAEONIA LACTIFLORA EXTRACTS FOR ANTI-OXIDATION, ANTI-INFLAMMATORY OR ANTI-WRINKLE, AND ME MANUFACTURING

The present invention relates to a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing a hydrolyzate of a peony flower extract, and a method for preparing the same.

Peony ( Paeonia lactiflora , Paeonia albiflora ) is a dicotyledonous perennial plant belonging to the peony and peony genus, and is mainly cultivated in China.

The peony is a perennial herbaceous plant that grows in mountainous areas. Several stems come out of a single stem and stand upright, and the height is about 60 cm, and the leaves and stems are hairless. Although there are several roots of the peony, it is thick and thick in the shape of a long, pointed cylinder at both ends. The leaves are alternate, and the veins and petioles are red. The flower of the peony is large, and the cultivated one has a size of about 10 cm in diameter and grows in the shape of an upside down egg.

The root of the peony contains a large amount of active ingredients such as benzoic acid and aspiragin, so it is mainly used as medicine for pain relief, abdominal pain, menstrual pain, amenorrhea, hematemesis, anemia, and bruises.

The flowers of the peony are large in size and have a good color, so they were mainly used for horticulture. For example, in Korean Patent Registration No. 10-0993603, a peony flower is collected, the flower is pre-treated as it is or lyophilized, ethanol is extracted, followed by filtration and concentration, and an extract having a whitening function is selected for analysis and cytotoxicity. Disclosed is a peony flower extract having a whitening function, prepared according to the experimental steps.

On the other hand, in the case of peony flower, as disclosed in the above registered patent, it is mainly known only for having a skin whitening effect, and its utility is low for purposes such as antioxidant, anti-inflammatory or anti-wrinkle. In addition, until now, only the degree of skin improvement by using the extract of the peony flower is considered, and there is a lack of research on the technique for effectively maximizing the efficacy of the peony flower.

Accordingly, the present applicant solves the above problems and implements skin improvement effects such as antioxidant, anti-inflammatory or anti-wrinkle using peony flower, but maximizes the efficacy of peony flower, so that the above effects are significantly improved By doing so, the present invention was completed.

Republic of Korea Patent No. 10-0993603

The first object of the present invention is to use the hydrolyzate of the peony flower extract, which is remarkably excellent in antioxidant, anti-inflammatory or anti-wrinkle effect, as an active ingredient by maximizing the efficacy of the peony flower by acid hydrolysis of the peony flower extract. To provide a cosmetic composition comprising.

A second object of the present invention is to provide a method for preparing a cosmetic composition comprising, as an active ingredient, a hydrolyzate of a peony flower extract, which is remarkably excellent in antioxidant, anti-inflammatory or anti-wrinkle effect, by acid hydrolysis of the peony flower extract. is to do

The object of the present invention is not limited to the technical problem as described above, and another technical problem may be derived from the following description.

In order to achieve the first object, the present invention provides a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle, comprising a hydrolyzate of peony flower extract as an active ingredient.

The hydrolyzate of the peony flower extract may be obtained by acid hydrolysis (Acidolysis) of the peony flower extract with an acidic substance.

The acidic material may include at least one selected from the group consisting of hydrochloric acid, citric acid, and vinegar.

The hydrolyzate of the peony flower extract may contain anthocyanidin.

The anthocyanidin may include peonidin.

In order to achieve the second object, the present invention comprises the steps of: (a) extracting a dried peony flower to obtain a peony flower extract; and (b) hydrolyzing the peony flower extract to obtain a hydrolyzate of the peony flower extract; It provides a method for preparing a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing a hydrolyzate of a peony flower extract, comprising a.

The cosmetic composition of the present invention includes a hydrolyzate obtained by acid hydrolysis of the peony flower extract, thereby achieving excellent antioxidant, anti-inflammatory or anti-wrinkle effects.

In particular, according to the acid hydrolysis of the peony flower extract in the present invention, better antioxidant, anti-inflammatory or anti-wrinkle effects can be realized than when the peony flower extract itself or the peony flower extract is hydrolyzed with an enzyme. .

In addition, according to the manufacturing method of the present invention, a cosmetic composition having a remarkably excellent antioxidant, anti-inflammatory or anti-wrinkle effect can be prepared by extracting the peony flower and performing acid hydrolysis of the extract.

1 is a view showing the HPLC test results of the hydrolyzate and pionidine prepared according to (1) of Experimental Example 2;
2 is a peony (flower) extract obtained according to Experimental Example 1, an acid hydrolyzate of a peony (flower) extract obtained according to a) of Experimental Example 2 using a 6N HCl aqueous solution and HPLC test results of peonidine it is one drawing
3 is a result of evaluation of DPPH radical scavenging ability according to Experimental Example 3.
4 is a cytotoxicity evaluation according to Experimental Example 4 (Test method: Raw264,7 MTT assay) results.
5 is an evaluation result of NO production inhibitory ability according to Experiment 4;
6 is a cytotoxicity evaluation according to Experimental Example 5 (test method: CCD-986sk MTT assay) results.
7 is an evaluation result of wrinkle improvement efficacy according to Experimental Example 5.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily implement them with reference to the accompanying drawings. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted.

The terms or words used in the specification and claims of the present invention are not to be construed as limited in their ordinary or dictionary meanings, and the inventor may appropriately define the concept of the term in order to best describe his invention. Based on the principle, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

In the entire specification of the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components unless otherwise stated. .

Throughout the specification of the present invention, "A and/or B" means A or B, or A and B.

Although the present invention has been specifically described below, the present invention is not limited thereto.

The present invention provides a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing a hydrolyzate of peony flower extract.

In an embodiment of the present invention, there is provided a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle, comprising a hydrolyzate of peony flower extract as an active ingredient.

Specifically, the cosmetic composition of this example extracts a peony flower to obtain a peony flower extract, and contains an acid hydrolyzate of the peony flower extract obtained by acid hydrolysis (Acidolysis) of the peony flower extract as an active ingredient Accordingly, more excellent antioxidant, anti-inflammatory or anti-wrinkle effects can be realized than when it contains a peony flower extract or an enzymatic hydrolyzate of a peony flower extract.

In particular, the acid hydrolyzate of the peony flower extract of this example contains a relatively high concentration of anthocyanidin, compared to the peony flower extract or the enzymatic hydrolyzate of the peony flower extract, so that it has significantly superior antioxidant, anti-inflammatory or The anti-wrinkle effect can be realized at the same time, and the overall skin condition can be improved.

Hereinafter, the hydrolyzate of the peony flower extract will be described in detail.

Peony ( Paeonia lactiflora , Paeonia albiflora ) is a dicotyledonous perennial plant belonging to the peony and peony genus, and is mainly cultivated in China. The peony is a perennial herbaceous plant that grows in mountainous areas. Several stems come out of a single stem and stand upright, and the height is about 60 cm, and the leaves and stems are hairless. Although there are several roots of the peony, it is thin and thick in the shape of a sharp cylinder with long ends. The leaves are alternate, and the veins and petioles are red. The flower of the peony is large, and the cultivated one has a size of about 10 cm in diameter and grows in the shape of an upside down egg.

The root of the peony contains a large amount of active ingredients such as benzoic acid and aspiragine, and is mainly used as medicine for pain relief, abdominal pain, menstrual pain, amenorrhea, hematemesis, anemia, and bruises. , mainly used for horticultural purposes.

The peony flower extract of this embodiment is an extract of the peony flower, and the peony flower extract is an extract obtained by extracting peony flowers, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared product of the extract, It includes extracts of all formulations that can be formed using the extract itself and the extract, such as purified products or mixtures thereof. Specifically, the peony flower extract of this embodiment may be prepared in the form of a dry powder after extraction, but is not limited thereto.

The extraction of the peony flower may be carried out by any known method, for example, hot water extraction, ethanol extraction, microwave extraction, supercritical extraction, and ultrasonic extraction by any one method selected from the group consisting of may be, but is not limited thereto.

The peony flower extract of this embodiment is a group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, dichloromethane, ethyl ether, hexane and 1,3-butylene glycol. It may mean extraction with an extraction solvent containing one or more selected from.

Specifically, the peony flower extract may be extracted with ethanol as an extraction solvent.

More specifically, the peony flower extract may be extracted with 70% (v/v) ethanol as an extraction solvent, but is not limited thereto.

The hydrolyzate of the peony flower extract of this embodiment may mean that obtained by hydrolyzing the peony flower extract by a known method, specifically, acid hydrolysis of the peony flower extract using an acidic substance (Acidolysis) It may mean that obtained by

In particular, the acid hydrolyzate of the peony flower extract according to this embodiment may contain anthocyanidin at a higher concentration than the peony flower extract or the enzymatic hydrolyzate of the peony flower extract, and thereby significantly excellent antioxidant , it is possible to implement an anti-inflammatory or anti-wrinkle effect.

In this case, the anthocyanidin may include peonidin, specifically, a peonidin-3-O-glucoside component, which is a glycoside contained in the peony flower extract. By being decomposed by this acid hydrolysis, the acid hydrolyzate of the peony flower extract can contain a high concentration of pionidine.

In this embodiment, for acid hydrolysis, the acidic material may include one or more selected from the group consisting of hydrochloric acid, citric acid and vinegar, but is not limited thereto.

Specifically, in this embodiment, hydrochloric acid may be included as the acidic material, and in this case, the concentration of anthocyanidins may be effectively increased by acid hydrolysis.

More specifically, in this embodiment, hydrochloric acid is included as the acidic material, but an aqueous hydrochloric acid solution having a normal concentration of 1 to 10 N may be included.

Most specifically, in this embodiment, hydrochloric acid is included as the acidic material, but an aqueous hydrochloric acid solution having a normal concentration of 5 to 7 N may be included.

In particular, when an aqueous solution having a normal concentration of 5 to 7 N of hydrochloric acid is included as the acidic material in this embodiment, the acid hydrolyzate of the peony flower extract of this embodiment may contain a high concentration of pionidine, Examples of cosmetic compositions can implement remarkably excellent antioxidant, anti-inflammatory or anti-wrinkle effects.

The cosmetic composition contains the hydrolyzate of the peony flower extract as an active ingredient, and along with excipients or additives that can be dermatologically used on the tongue, softening lotion, nutrient lotion, astringent lotion, skin, lotion, essence, cream, massage cream, pack, It may be formed in any one formulation selected from the group consisting of makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream, and cleansing water, but is not limited thereto, and may be formed in any known formulation.

In this case, the cosmetic composition may be implemented in various formulations by further including an excipient or additive.

For example, the excipients or additives include surfactants, emulsifiers, emulsifying aids, silicone polymers, pigments, pigments, fragrances, preservatives, bactericides, oxidation stabilizers, organic solvents, thickeners, emollients, antioxidants, free radical scavengers, opacity Topicals, stabilizers, emollients, defoamers, humectants, vitamins, insect repellents, preservatives, anti-inflammatory agents, fillers, polymers, propellants, basifying agents, acidifying agents, colorants, thickening agents, skin conditioning agents, whitening agents, antioxidants, anti-wrinkle agents and It may include one or more selected from the group consisting of solubilizers, but is not limited thereto.

In addition, there is provided a method for preparing a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing a hydrolyzate of peony flower extract.

In another embodiment of the present invention, (a) extracting the dried peony flower, obtaining a peony flower extract; and (b) hydrolyzing the peony flower extract to obtain a hydrolyzate of the peony flower extract; It provides a method for producing a cosmetic composition for anti-oxidation, anti-inflammatory or anti-wrinkle, comprising a.

Hereinafter, the manufacturing method will be described in detail step by step.

(a) extracting the dried peony flower to obtain a peony flower extract

In step (a) of this example, the dried peony flower can be extracted to obtain a peony flower extract. Specifically, in step (a) of this example, the dried peony flower is extracted with an extraction solvent, concentrated under reduced pressure, and then freeze-dried to obtain a peony flower extract in powder form.

The extraction of the peony flower may be carried out by any known method, for example, hot water extraction, ethanol extraction, microwave extraction, supercritical extraction, and ultrasonic extraction by any one method selected from the group consisting of may be, but is not limited thereto.

The extraction solvent includes at least one selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, dichloromethane, ethyl ether, hexane and 1,3-butylene glycol can do.

Specifically, the peony flower extract may be extracted with ethanol as an extraction solvent.

More specifically, the peony flower extract may be extracted with 70% (v/v) ethanol as an extraction solvent, but is not limited thereto. For example, the peony flower extract may be obtained by adding 70% (v/v) ethanol of 20 to 40 times the weight of the dried peony flower as an extraction solvent.

Step (a) of this embodiment may be carried out at room temperature conditions, but is not limited thereto.

(b) hydrolyzing the peony flower extract to obtain a hydrolyzate of the peony flower extract

In step (b) of this example, the hydrolyzate of the peony flower extract obtained in step (a) can be hydrolyzed to obtain a hydrolyzate of the peony flower extract, and specifically, in step (b) of this example, step (a) Acid hydrolysis of the peony flower extract obtained in

In particular, the acid hydrolyzate of the peony flower extract may contain anthocyanidin at a higher concentration than the enzymatic hydrolyzate of the peony flower extract or the peony flower extract, and thereby significantly excellent antioxidant, anti-inflammatory or anti-wrinkle effect can be implemented.

In this case, the anthocyanidin may include peonidin, specifically, a peonidin-3-O-glucoside component, which is a glycoside contained in the peony flower extract. By being decomposed by this acid hydrolysis, the acid hydrolyzate of the peony flower extract can contain a high concentration of pionidine.

In step (b) of this embodiment, acid hydrolysis of the peony flower extract is carried out by mixing and reacting the peony flower extract and an acidic substance, and as the acidic substance used for the acid hydrolysis, hydrochloric acid, citric acid ( Citric acid) and may include one or more selected from the group consisting of vinegar (vinegar), but is not limited thereto.

Specifically, hydrochloric acid may be included as the acidic material, and in this case, the concentration of anthocyanidins may be effectively increased by acid hydrolysis.

More specifically, in this embodiment, hydrochloric acid is included as the acidic material, but an aqueous hydrochloric acid solution having a normal concentration of 1 to 10 N may be included.

Most specifically, in this embodiment, hydrochloric acid is included as the acidic material, but an aqueous hydrochloric acid solution having a normal concentration of 5 to 7 N may be included. In particular, in this embodiment, when an aqueous solution having a normal concentration of hydrochloric acid of 5 to 7 N as the acidic material is included, the acid hydrolyzate of the peony flower extract obtained according to step (b) contains a high concentration of pionidine. Thus, the cosmetic composition of the present embodiment can implement a remarkably excellent antioxidant, anti-inflammatory or anti-wrinkle effect.

Step (b) of this embodiment may be carried out at 40 ~ 95 ℃, specifically 70 ~ 90 ℃, more specifically 80 ~ 90 ℃. In particular, when step (b) of this example is carried out at 80 ~ 90 ℃, it is possible to obtain an acid hydrolyzate of the peony flower extract having an increased content of peonidine by acid hydrolysis, so that excellent antioxidant, anti-inflammatory or An anti-wrinkle effect can be realized.

If step (b) of this embodiment is carried out at less than 40 ℃, hydrolysis is not sufficiently carried out, the effect of increasing the content of pionidine is insignificant, and thus the improvement of the antioxidant, anti-inflammatory or anti-wrinkle effect is also insignificant. can On the other hand, when step (b) of this example is carried out at more than 95 ° C., a side reaction is carried out or moisture evaporation occurs, so that hydrolysis may not be performed properly, and thus, the effect of increasing the content of pionidine is reduced. As it is insignificant, the antioxidant, anti-inflammatory or anti-wrinkle effect may be reduced.

Step (b) of this embodiment is carried out at 40 ~ 95 ℃, it may be carried out for 30 ~ 120 minutes, specifically 50 ~ 70 minutes. In particular, when step (b) of this example is carried out at 40 to 95 ° C., and is carried out for 50 to 70 minutes, an acid hydrolyzate of the peony flower extract having an increased content of peonidine by acid hydrolysis can be obtained. Therefore, excellent antioxidant, anti-inflammatory or anti-wrinkle effects can be realized.

If step (b) of this embodiment is carried out for less than 30 minutes, hydrolysis is not sufficiently performed, the effect of increasing the content of pionidine is insignificant, and the antioxidant, anti-inflammatory or anti-wrinkle effect may be reduced. On the other hand, when step (b) of this embodiment is carried out for more than 120 minutes, a side reaction may occur, or the degree of improvement of the effect over time may be insignificant, which may not be economical.

In addition, in addition to the steps (a) and (b), a known pre-treatment or post-treatment may be carried out, which is not particularly limited.

In addition, by mixing with an excipient or additive as necessary after performing step (b), the step of forming a cosmetic composition having a specific formulation may be carried out.

The manufacturing method of the cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing the hydrolyzate of the peony flower extract follows all the contents of the cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing the hydrolyzate of the peony flower extract.

Hereinafter, through Examples, Comparative Examples, and Experimental Examples, a cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle containing a hydrolyzate of the peony flower extract of the present invention and a manufacturing method thereof will be described in detail. These examples are only for illustrating the present invention, and therefore, the scope of the present invention should not be construed as being limited by these examples.

[Experimental example]

Experimental Example 1: Preparation of peony flower extract

20 g of dried flowers of peony and 600 g of 70% ethanol were mixed, and extraction was performed by repeating immersion at room temperature 3 times. Thereafter, the extracted product was concentrated under reduced pressure and then freeze-dried (condition: 5 Torr, 72 hr) to obtain a peony (flower) extract.

Experimental Example 2: Preparation of hydrolyzate of peony flower extract

(1) Preparation of hydrolyzate

The peony (flower) extract prepared in Experimental Example 1 was hydrolyzed according to each of the following a) to e) to obtain a hydrolyzate.

a) Acid hydrolysis using 6N HCl aqueous solution: After mixing 1 g of peony (flower) extract and 10 ml of 6 N HCl, the mixture was reacted at about 90° C. for 1 hour to obtain a hydrolyzate.

b) Acid hydrolysis using 1N HCl: After mixing 1 g of peony (flower) extract and 10 ml of 1 N HCl, the mixture was reacted at about 90° C. for 1 hour to obtain a hydrolyzate.

c) Acid hydrolysis using citric acid: After mixing 1 g of peony (flower) extract, 1 g of citric acid and 10 ml of purified water, the mixture was reacted at about 90° C. for 1 hour to obtain a hydrolyzate.

d) Acid hydrolysis using vinegar: 1 g of peony (flower) extract, 5 ml of vinegar, and 4 ml of purified water were mixed and reacted at about 90° C. for 1 hour to obtain a hydrolyzate.

e) Acid hydrolysis using enzyme: 1 g of peony (flower) extract, 1 ml of Cellulase, and 10 ml of purified water were mixed and reacted at about 40° C. for 1 hour to obtain a hydrolyzate.

(2) Result of component analysis of hydrolyzate

Components a) to e) each of the hydrolyzate and pionidine (concentration: 100 ppm) were analyzed, and the results are shown in FIG. 1 .

The component analysis was performed by HPLC analysis under the following conditions.

At this time, in FIG. 1, substances used for hydrolysis are described for each corresponding graph.

- Column : Phenomenon Luna C18 250 x 4.6mm

- Solvent A : 1% Formic acid in water

- Solvent B : 1% Formic acid in acetonitrile

- Injection volume: 10 μl

- Oven temperature : 35 ℃

- Flow rate: 1 ml/min

- Microwave: 525 nm

Referring to FIG. 1 , it can be confirmed that the same peak as that of pionidine is analyzed when acid hydrolysis is performed using 6N HCl aqueous solution.

On the other hand, it can be seen that when an enzyme is used, the same peak as that of pionidine is not analyzed. This means that when the peony flower extract is enzymatically hydrolyzed rather than acid hydrolyzed, the content of pionidine cannot be increased. means difficulty.

(3) Result of component analysis of extract and hydrolyzate

Comparison of the acid hydrolyzate and peonidine (concentration: 100 ppm) of the peony (flower) extract obtained according to Experimental Example 1 and the 6N HCl aqueous solution obtained according to a) of Experimental Example 2 For this purpose, component analysis results were performed, and the results are shown in FIG. 2 and Table 1.

At this time, the component analysis proceeded in the same manner as above.

2 and Table 1, it can be confirmed that the same peak as pionidine is analyzed when acid hydrolysis is performed using 6N HCl aqueous solution, but the peony flower extract itself, which is not hydrolyzed, is It can be confirmed that the same peak is not analyzed.

In particular, if you look at the peak analysis result of the peony flower extract, the glycoside peonidine-3-O-glucoside component is analyzed. It is confirmed that the content of onidine is increased.

Specifically, referring to document 1 (Parker, Shefton, et al. "A pharmacological review of bioactive constituents of Paeonia lactiflora Pallas and Paeonia veitchii Lynch." Phytotherapy Research 30.9 (2016): 1445-1473.), peony extract in peony It can be seen that, as Peonin, a glycoside of Dean, a peonidin-3,5-diglucoside component is contained, and document 2 (Jia, Ni, et al. "Identification and characterization of anthocyanins by high-performance liquid chromatography-electrospray ionization-mass spectrometry in herbaceous peony species." Journal of the American Society for Horticultural Science 133.3 (2008): 418-426.) As it is confirmed that anthocyanin components including din-3-O-glucoside are detected, in view of the above, sugar is separated from pionidine-3-O-glucoside by acid hydrolysis, and pionidine It can be judged that the content of

That is, it can be confirmed that the content of pionidine is increased through acid hydrolysis using 6N HCl aqueous solution. -Since it has a smaller molecular size than glucoside, it can show a relatively high skin absorption rate, so it can have an excellent skin improvement effect.

Through Experimental Examples 1 and 2, it was confirmed that the acid hydrolyzate of the peony flower extract using 6N HCl aqueous solution could contain a higher content of peonidine than the peony flower extract.

Therefore, in the following, using the acid hydrolyzate of the peony flower extract using a 6N HCl aqueous solution, the efficacy of antioxidant, anti-inflammatory and anti-wrinkle was evaluated, and the results were shown.

Experimental Example 3: Antioxidant efficacy evaluation

The acid hydrolyzate of the peony (flower) extract obtained according to Experimental Example 1 and the 6N HCl aqueous solution obtained according to a) of Experimental Example 2 was prepared by concentration, and then 0.5 mM DPPH solution and a sample were prepared. After mixing 1:1, the mixture was reacted at room temperature for 30 minutes. After that, absorbance at 540 nm was measured using ELISA (Bio-Rad, USA), and the antioxidant effect (DPPH radical scavenging ability _{) was '100 - [{for each sample (OD DPPH -OD ethanol} )}/ {dH ₂ O (OD) _DPPH -OD _ethanol )} X 100]', and the results are shown in FIG. 3 and Table 2.

In this case, the concentration unit in Table 2 is μg/mL.

Referring to Table 2 and Figure 3, it can be seen that in the case of the acid hydrolyzate of the peony (flower) extract using 6N HCl aqueous solution, the DPPH radical scavenging ability is superior to that of the simple extract.

In particular, the DHHP test is a colorimetric test that quantifies the degree to which a purple DPPH solution is reduced by an antioxidant and changes from its intrinsic purple to yellow. As a result of converting absorbance into DPPH scavenging ability, It can be seen that the acid hydrolyzate exhibits almost 100% scavenging ability from the point in time when the sample concentration is 78.125 μg/mL or more.

That is, it can be seen that the acid hydrolyzate of peony (flower) extract using 6N HCl aqueous solution can realize excellent antioxidant effect as the content of pionidine increases.

Experimental Example 4: Evaluation of anti-inflammatory efficacy

(1) Assessment of cytotoxicity

The cytotoxicity test of the peony (flower) extract obtained according to Experimental Example 1 and the acid hydrolyzate of the peony (flower) extract obtained according to a) of Experimental Example 2 using 6N HCl aqueous solution was performed according to Raw264,7 MTT assay. carried out, and the results are shown in FIG. 4 .

Specifically, the Raw264,7 MTT assay was performed as follows. First, 100 μL of the RAW264.7 cell line was dispensed at an appropriate concentration per well in a 96-well plate, and cultured for 24 hours at _{37° C. and 5% CO 2 incubator conditions.} Then, after processing the samples in the cultured cells by concentration, 37 ° C and 5% CO ₂ After incubation for 24 hours under the incubator conditions, 3 hours before the completion of the culture, 10 μL of 5 mg / mL MTT treatment, the same conditions for 3 hours cultured.

Following the completion of the culture, the culture solution was discarded, 100 μL of DMSO was dispensed, stirred at room temperature for 10 minutes, and absorbance was measured at 570 nm using ELISA (Bio-Rad, USA), and the viability was calculated as ‘(Experimental group absorbance/control group). Absorbance) X 100' was derived according to the formula, and the results are shown in FIG. 4 .

In this case, gray shades in FIG. 4 indicate toxicity with the naked eye.

4, it can be seen that the peony extract does not show cytotoxicity at a concentration of 100 μg/mL or less, and it can be seen that the acid hydrolyzate of the peony extract does not show cytotoxicity at a concentration of 100 μg/mL or less.

(2) Anti-inflammatory evaluation

The NO production inhibitory effect of the acid hydrolyzate of the peony (flower) extract obtained according to Experimental Example 1 and the 6N HCl aqueous solution obtained according to a) of Experimental Example 2 was evaluated, and the results are shown in Table 3 and FIG. 5 . At this time, FIG. 5 shows the NO concentration evaluated as follows as a graph, and in Table 3, the measured NO concentration is calculated according to the following formula, and the anti-inflammatory effect is described.

Specifically, a NO assay was performed to evaluate the NO production inhibitory ability. First, 100 μL of RAW264.7 cell line was dispensed into a 96-well plate at an appropriate concentration per well, and cultured for 24 hours at _{37° C. and 5% CO 2 incubator conditions.} Then, in order to induce the production of NO in the cultured cells, the medium was exchanged with a medium containing 1 μg/mL of lipopolysaccharide (LPS), and the sample was diluted by concentration using the cell medium, and the cells were treated , and cultured for 24 hours.

For the amount of NO produced, 100 μL of cell culture supernatant and 100 μL of Griess reagent were mixed, reacted for 10 minutes in a 96-well plate, and absorbance was measured at 540 nm using ELISA (Bio-Rad, USA), and the amount of NO was measured with nitric acid. After calculating by comparison with the standard curve for each concentration of sodium (NaNO2), '100 - [{NO conc. (μM) comparison group 2 or experimental group}/ { NO conc. (μM) comparison group 1} X 100]' formula with anti-inflammatory effect.

Referring to Table 3 and Figure 5, it can be seen that in the case of the acid hydrolyzate of the peony (flower) extract using the 6N HCl aqueous solution, the NO production inhibitory effect is more excellent than that of the simple extract.

Specifically, when the processing concentration of the sample is 50 μg/mL or more, more specifically, when the processing concentration of the sample is 50 to 100 μg/mL, the acid hydrolyzate of the peony (flower) extract using 6N HCl aqueous solution is simple It can be seen that the extract shows significantly better NO removal efficiency than the extract. In particular, when the treatment concentration is 100 μg/mL, it can be seen that the acid hydrolyzate of the peony (flower) extract using the 6N HCl aqueous solution shows a NO removal efficiency that is about 1.5 times higher than that of the simple extract.

That is, it can be seen that the acid hydrolyzate of peony (flower) extract using 6N HCl aqueous solution can realize excellent anti-inflammatory effect as the content of pionidine increases.

Experimental Example 5: Anti-wrinkle evaluation

(1) Assessment of cytotoxicity

The cytotoxicity test of the peony (flower) extract obtained according to Experimental Example 1 and the acid hydrolyzate of the peony (flower) extract obtained according to a) of Experimental Example 2 using 6N HCl aqueous solution was performed according to CCD-986sk MTT assay. was carried out, and the results are shown in FIG. 6 .

The CCD-986sk MTT assay was performed as follows. First, 200 μL of the CCD-986sk cell line was dispensed at an appropriate concentration per well in a 96-well plate, and _{cultured at 37 ° C., 5% CO 2 in an} incubator condition for 24 hours. Then, the cultured cells were treated with samples by concentration, 37 ° C., 5% CO ₂ Incubated for 24 hours under incubator conditions, and 20 μL of 5 mg/mL MTT was treated 3 hours before completion of culture, and then cultured under the same conditions for 3 hours. . After the culture was completed, the culture solution was discarded, and 150 μL of DMSO was dispensed, stirred at room temperature for 10 minutes, and absorbance was measured at 570 nm using ELISA (Bio-Rad, USA).

Thereafter, the survival rate was derived as '(experimental group absorbance/control group absorbance) X 100 formula', and the results are shown in FIG. 6 .

In this case, gray shades in FIG. 6 indicate toxicity with the naked eye.

Referring to FIG. 6 , it can be seen that the peony extract does not show cytotoxicity at a concentration of 100 μg/mL or less, and the acid hydrolyzate of the peony extract does not exhibit cytotoxicity at a concentration of 6.25 μg/mL or less.

(2) Anti-wrinkle evaluation

Using the acid hydrolyzate of the peony (flower) extract obtained according to Experimental Example 1 and the peony (flower) extract obtained according to a) of Experimental Example 2 using 6N HCl aqueous solution, according to CCD-986sk UVA assay, wrinkles Efficacy was evaluated, and the results are shown in Table 4 and FIG. 7 .

Specifically, the CCD-986sk UVA assay was performed as follows. First, 200 μL of the CCD-986sk cell line was dispensed at an appropriate concentration per well in a 96-well plate, and cultured for 24 hours at _{37° C. and 5% CO 2 in an incubator condition.} Subsequently, the cultured cells were irradiated with UVA 30 J/cm ² using a UV irradiation system, and samples were treated with each concentration in the UV-treated cells, and incubated for 24 hours at _{37° C. and 5% CO 2 incubator conditions.} At this time, 3 hours before the completion of the culture, 10 μL of 5 mg/mL MTT was treated, and then cultured under the same conditions for 3 hours.

After the culture was completed, the culture solution was discarded, and 150 μL of DMSO was dispensed, followed by stirring at room temperature for 10 minutes. Thereafter, absorbance was measured at 570 nm using ELISA (Bio-Rad, USA), and the survival rate was derived as '(absorbance of experimental group / absorbance of control group) X 100', and the results are shown in Table 4 and FIG. 7 .

Referring to Table 4 and Figure 7, in the case of the acid hydrolyzate of the peony (flower) extract using 6N HCl aqueous solution, it can be seen that the anti-wrinkle effect is superior to that of the simple extract. Specifically, when the treatment concentration is 6.25 μg/mL, it can be seen that the acid hydrolyzate of the peony (flower) extract using a 6N HCl aqueous solution shows an anti-wrinkle effect about 10 times higher than that of the simple extract. In particular, since UV rays cause wrinkles of the skin, reduce cell division, and decrease the amount of collagen and elastin to reduce anti-wrinkle efficacy, according to CCD-986sk UVA assay, through improvement of dermal cell proliferation damaged by UVA , it is possible to implement skin wrinkle improvement.

That is, it can be seen that the acid hydrolyzate of the peony (flower) extract using a 6N HCl aqueous solution can realize an excellent anti-wrinkle effect as the content of pionidine increases.

Considering the above contents, the acid hydrolyzate of peony flower extract contains peonidine in a higher content than the enzymatic hydrolyzate of peony flower extract or peony flower extract, and peonidine-3-O-glucoside It can be seen that pionidine, which has a smaller molecular size, is effectively absorbed into the skin, and realizes better antioxidant, anti-inflammatory and anti-wrinkle effects.

As described above, the description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can easily transform into other specific forms without changing the technical spirit or essential features of the present invention. You will understand. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims rather than the above detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention. do.

Claims (6)

As an antioxidant, anti-inflammatory or anti-wrinkle cosmetic composition comprising a hydrolyzate of peony flower extract as an active ingredient,
The peony flower extract is an ethanol extract of peony flower, and the hydrolyzate of the peony flower extract is obtained by acid hydrolysis of the peony flower extract with an acidic substance,
The acidic material is an antioxidant, anti-inflammatory or anti-wrinkle cosmetic composition, characterized in that it comprises an aqueous hydrochloric acid solution having a normal concentration of 5 to 7 N.
delete delete The method of claim 1,
The hydrolyzate of the peony flower extract is
A cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle, characterized in that it contains anthocyanidin.
5. The method of claim 4,
The anthocyanidins are
A cosmetic composition for antioxidant, anti-inflammatory or anti-wrinkle, characterized in that it contains peonidin.
(a) extracting the dried peony flower to obtain a peony flower extract; and
(b) hydrolyzing the peony flower extract to obtain a hydrolyzate of the peony flower extract; including,
The peony flower extract is an ethanol extract of peony flower, and the hydrolyzate of the peony flower extract is obtained by acid hydrolysis of the peony flower extract with an acidic substance,
The acidic material is a method for producing an antioxidant, anti-inflammatory or anti-wrinkle cosmetic composition comprising a hydrolyzate of peony flower extract, characterized in that it comprises an aqueous hydrochloric acid solution having a normal concentration of 5 to 7 N.

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