KR102197123B1 - Composition Containing Absolute of Patrinia scabiosaefolia Flower - Google Patents

Abstract

The present invention relates to a method for extracting essential oil from Matari flower (Patrinia scabiosaefolia flower), and a composition for external application for skin and a cosmetic composition comprising the essential oil prepared therefrom, and the essential oil extracted from the Matari flower of the present invention is applied to epidermal keratinocytes. While not showing toxicity, it has improved the proliferation and migration of epidermal keratinocytes by controlling extracellular/internal signaling, and has the effect of promoting collagen formation in epidermal keratinocytes, so the essential oil is used for epidermal keratinocytes. It can be used as an external preparation for skin composition and a cosmetic composition for skin regeneration and wound healing by inducing proliferation and migration of

Description

Composition Containing Essential Oil Derived from Matari Flower {Composition Containing Absolute of Patrinia scabiosaefolia Flower}

The present invention relates to a composition comprising essential oil derived from Matari flowers ( Patrinia scabiosaefolia flower), and specifically, to a method for extracting essential oil from Matari flowers, and a composition for external application for skin and a cosmetic composition comprising the essential oil prepared therefrom. will be.

The skin is the tissue that covers the outermost part of the human body. It protects the human body and acts as a barrier to protect the body from external chemical and physical shocks. The skin is composed of three layers: epidermis, dermis, and subcutis, and 90% of the epidermis located in the outermost layer is composed of keratinocytes. When the skin is aged by ultraviolet rays or stress or damaged by wounds, its function is weakened, and the skin regeneration process begins. The skin regeneration process begins with an acute inflammatory reaction, proliferation, migration, and proliferation of epidermal cells, the formation of new blood vessels, and the contraction of connective tissue through the synthesis of collagen. At this time, the migration and proliferation of keratinocytes, which occupy more than 90% of the epidermis, not only provides wound healing and skin regeneration, but also plays an important role in enhancing the elasticity of the skin and forming the basement membrane by inducing collagen synthesis.

On the other hand, plant-derived natural essential oils are volatile chemicals that exhibit pharmacological effects on the human body among secondary metabolites of plants, and are mainly composed of various organic compounds such as terpenes, alcohols, aldehydes, esters, and ketones. The aromatic components of these natural essential oils not only relieve stress, recover fatigue, and relax muscles through the skin, circulatory system, and olfactory system, but also exhibit antibacterial, immunity, and anti-allergic therapeutic effects. Not only has it been used for treatment and prevention, but it is also used as an ingredient in food flavors and cosmetics.

On the other hand, Matari ( Pat r inia scabiosaefolia ) is a perennial herbaceous plant belonging to the Matari family. It grows wildly in mountains and fields in Korea, Japan, China, and eastern Siberia, and blooms in July-August. In oriental medicine, Matari is said to be excellent in anti-inflammatory action against eye diseases, purulent streptococcus, edema, and hypoplasia, and Matari outpost has been used as a herbal medicine for a long time. Matari's outposts, roots, and rhizomes contain a large amount of essential oil, and it has been reported that various active ingredients such as saponin, coumarin, carbohydrates and trace alkaloids are contained. Studies on the physiological activity of Matari have been reported to have antioxidant activity, immune system regulation activity, anti-cell proliferation, anti-microbial activity, anti-tumor activity, anti-viral, and skin atopy improvement activity.

However, until now, studies on skin physiological activity using matari have been insufficient, and in particular, there are no studies using essential oils contained in matari. While studying the in vitro skin restoration effect by extracting essential oil from Matari flowers, the present inventors confirmed that the essential oil derived from Matari flowers induces the proliferation and migration of keratinocytes in the skin epidermis to show a skin restoration effect, and completed the present invention. I did.

KR 10-2011-0001538 A

In order to solve the above problems, the present invention has completed the present invention by identifying the constituents of the essential oil derived from Matari flowers, and confirming its skin elasticity enhancement, skin regeneration, and wound healing efficacy. In addition, the present invention is to provide a method of manufacturing the essential oil of Matari flowers.

In order to solve the above problems, the present invention provides a composition for external application for skin comprising essential oil extracted from Patrinia scabiosaefolia flower.

In one embodiment of the present invention, the essential oil may be extracted by an organic solvent extraction method using a volatile solvent, and the concrete extracted by the organic solvent extraction method is extracted with alcohol to remove insoluble wax. It may be essential oil (Absolute).

In one embodiment of the present invention, the matari flower may not be dried.

In one embodiment of the present invention, the essential oil contains a volatile organic compound, for example, Ursa-9(11),12-dien-3-one, Ursa-9(11),12-dien-3 -ol, beta-sitosterol (β-Sitosterol), aristolone (Aristolone), may include any one or more volatile organic compounds selected from the group consisting of alpha-amirin (α-Amyrin).

In an embodiment of the present invention, the essential oil may be included in the range of 1 to 200 μg/mL, for example, may be included in the range of 10 to 200 μg/mL.

In one embodiment of the present invention, the essential oil may induce proliferation and migration of keratinocytes in the skin epidermis. Specifically, the essential oil may induce one or more phosphorylation processes or signaling processes selected from the group consisting of epidermal keratinocyte extracellular signal regulation kinase (ERK1/2) and intracellular serine/threonine protein kinase (Akt).

In addition, the present invention provides a cosmetic composition for skin regeneration comprising the essential oil extracted from Matari flowers as an active ingredient.

In addition, the present invention provides a method for producing a Matari flower essential oil.

The present invention identifies the components of essential oil extracted from Matari flowers, and the essential oil extracted from Matari flowers demonstrates its skin regeneration and wound healing efficacy without showing cytotoxicity to epidermal keratinocytes. It has an excellent effect on skin regeneration by inducing proliferation and/or migration. In addition, the essential oil extracted from the Matari flower according to the present invention has improved skin elasticity and wound healing effect, and thus can be variously applied as a cosmetic or an external preparation for skin.

1 is a view showing a process of extracting essential oil (absolute; Absolute) from matari flowers in an embodiment of the present invention.
2 is a diagram showing the results of component analysis of essential oil derived from Matari flowers in an embodiment of the present invention.
3 is a diagram illustrating the cytotoxicity of essential oils derived from Matari flowers in an embodiment of the present invention to skin epidermal keratinocytes.
4 is a diagram illustrating the effect of promoting the proliferation of epidermal keratinocytes of the essential oil derived from Matari flowers in an embodiment of the present invention.
5 is a diagram illustrating the effect of promoting the movement of skin epidermal keratinocytes by the essential oil derived from Matari flowers in an embodiment of the present invention.
6 is an essential oil derived from Matari flowers in an embodiment of the present invention
(A) An image showing the effect on the phosphorylation of epidermal keratinocytes extracellular signal regulation kinase (ERK 1/2) and intracellular serine/threonine protein kinase (Akt);
(B) the degree of phosphorylation of the quantified epidermal keratinocyte extracellular signaling kinase (ERK1/2); And
(C) It is a diagram confirming the degree of phosphorylation of serine/threonine protein kinase (Akt) in quantified epidermal keratinocytes.
7 is an image showing the effect of the essential oil derived from Matari flowers on the out growth of collagen sprouting in skin epidermal keratinocytes according to an embodiment of the present invention, and a diagram showing the quantification thereof.

Hereinafter, the present invention will be described in detail as an embodiment of the present invention with reference to the accompanying drawings. However, the following implementation examples are presented as examples of the present invention, and when it is determined that a detailed description of a technique or configuration well known to those skilled in the art may unnecessarily obscure the subject matter of the present invention, the detailed description may be omitted. However, the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of equivalents interpreted therefrom and description of the claims to be described later.

In addition, terms used in the present specification are terms used to properly express preferred embodiments of the present invention, which may vary according to the intention of users or operators, or customs in the field to which the present invention belongs. Therefore, the definitions of these terms should be made based on the contents throughout this specification. Throughout the specification, when a certain part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise stated.

Throughout this specification, the'%' used to indicate the concentration of a specific substance is (w/w)% for solids/solids, (w/v)% for solids/liquids, and Liquid/liquid is (v/v) %.

In one aspect, it relates to a composition for external application for skin comprising essential oil extracted from flowers of Matari flowers ( Patrinia scabiosaefolia flower).

In one embodiment, the essential oil may be prepared by extracting the matari flower by an organic solvent extraction method to obtain concrete, and dissolving it in an organic solvent and filtering to remove the insoluble wax to obtain absolute.

In one embodiment, the essential oil extracted from the matari flower of the present invention is immersed in the matari flower in an organic solvent; Evaporating and concentrating the immersion liquid to obtain concrete; Dissolving concrete in an organic solvent and filtering to remove insoluble wax; And it can be prepared by separating and purifying the absolute through evaporation.

The organic solvent extraction method and the method of separating the absolute from the extract may include all methods that can be modified by one of ordinary skill in the art.

In one embodiment, the essential oil extracted from Matari flowers is Ursa-9 (11), 12-dien-3-one, Ursa-9 (11), 12-dien-3-ol, beta-sitosterol (β-Sitosterol), It may contain at least one component selected from the group consisting of Aristolone and alpha-Amyrin.

In one embodiment, the matari flower may be a bud just before flowering, and may be an undried flower.

In one embodiment, the composition for external application for skin of the present invention may be for epidermal keratinocyte proliferation, regeneration, and collagen formation, which is the elasticity, regeneration, and/ Or it may be useful for wound healing.

In one embodiment, the composition for external application for skin of the present invention may be used in a concentration of 10 to 150 μg/mL of essential oil extracted from Matari flowers, and it is best to include it in a concentration of 10 to 150 μg/mL for skin regeneration and wound healing. It is more preferable because it has an effect.

The term "essential oil" as used in the present invention is a collective term for aromatic oils (volatile substances) extracted and separated from plants (plant leaves, stems, fruits, flowers, roots, etc.), and extracts, extraction methods, uses and purposes According to the extraction method, essential oils derived from natural products are classified into essential oils, absolutes, and concretes.

The term "extract" as used in the present invention means an active ingredient separated from a natural product, that is, a substance exhibiting a desired activity. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes a dry powder of the extract or any form formulated using the same. In addition, the extract includes fractions of the extract that has undergone the extraction process. The extraction method of the extract is not particularly limited, and may be extracted by, for example, stirring extraction, shaking extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction. As the extraction solvent, a polar solvent such as water or a C ₁ -C ₄ lower alcohol, a non-polar solvent such as hexane, chloroform, dichloromethane or ethyl acetate, or a mixture of two or more thereof may be used.

In one aspect, the present invention relates to a composition for external application for skin comprising essential oil extracted from Matari flowers.

In one aspect, the present invention relates to a cosmetic composition comprising essential oil extracted from Matari flowers.

The cosmetic composition of the present invention may be prepared by including a cosmetically effective amount of essential oil extracted from the Matari flower of the present invention and a cosmetically acceptable carrier.

As used herein, the term "cosmetically effective amount" means an amount sufficient to achieve the effect of skin regeneration through the proliferation of epidermal keratinocytes of the composition of the present invention described above.

The cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, such as solutions, gels, solids, dough anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, for example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing It can be formulated into cosmetics such as cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

When the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, as a carrier component, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be used.

The cosmetic composition of the present invention is skin, lotion, cream, essence, pack, foundation, color cosmetics, sun cream, two-way cake, face powder, compact, makeup base, skin cover, eye shadow, lipstick, lip gloss, lip fix, eyebrow pencil , It can be applied to cosmetics such as lotion and detergents such as shampoo and soap.

In addition to the essential oil extracted from the Matari flower, the cosmetic composition according to an embodiment of the present invention may additionally include functional additives and ingredients included in general cosmetic compositions. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

In addition to the above functional additives, the cosmetic composition of the present invention may further contain ingredients contained in a general cosmetic composition, if necessary. Other ingredients included include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation Examples include accelerators, cooling agents, restrictors, and purified water.

In one aspect, the present invention includes immersing the matari flower in an organic solvent, evaporating and concentrating the immersion liquid to obtain concrete, dissolving and filtering the concrete in an organic solvent to remove insoluble wax, and separating and purifying the absolute It relates to a method of manufacturing the essential oil of Matari flowers.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the examples or experimental examples described below in detail. Hereinafter, the present invention will be described in detail by examples or experimental examples. However, these examples or experimental examples are intended to specifically describe the present invention, and the scope of the present invention is not limited to these examples.

Example 1. Preparation of Matari Flower Essential Oil

Matari flowers were collected and washed, extracted by immersing in hexane for 60 minutes without drying, and then concentrated with a rotary evaporator to obtain solid concrete. Thereafter, alcohol was added to the solid concrete to react for 12 hours, and after removing the insoluble wax through a vacuum cooling filter, separation/purification was performed with a rotary evaporator to obtain a matari flower absolute (see FIG. 1 ). ). The prepared Matari flower absolute was stored in a low temperature storage at 4°C.

Example 2. Analysis of components of essential oil of Matari flowers

Using a gas chromatography/mass spectrometer (6890N GC/5975i MS, Agilent, USA), the components of the Matari flower absolute were determined by the retention indices (RI) of the volatiles, the retention time of the standard, and the mass spectrum of the volatiles. The mass spectrum of the Wiley 7Nist05 library was compared and analyzed. Specifically, DB5-MS (30m×50μm, 0.25μm) was used as the GC/MS column, and the carrier gas was analyzed by flowing at 1 mL/min using helium. The temperature of the oven was maintained at 40℃-2℃/min-230℃-5℃/min-300℃ (5 minutes), and the temperature of the injection was analyzed at 280℃. In addition, the temperature of the interface was analyzed at 300℃, the temperature of the ion source was 230℃, the temperature of the analyzer was 150℃, and the mass range was analyzed at 40~800m/z. RI was determined under the same conditions as the sample using C6 ~ C24 (n-alkane).

As a result, it was confirmed that the Matari flower absolute prepared in Example 1 was composed of a total of five components, alpha-Amyrin, Aristolone, and Ursa-9(11). ,12-dien-3-ol, beta-sitosterol, Ursa-9(11), 12-dien-3-one showed the highest content in the order.

Example 3. Confirmation of Cytotoxicity of Essential Oil Derived from Matari Flower

It was confirmed whether the Matari flower essential oil (absolut) obtained in Example 1 exhibits toxicity to the skin epidermal keratinocytes. Specifically, human skin-derived epidermal keratinocytes (HaCat) were cultured in DMEM medium containing 10% fetal bovine serum, and used in the experimental examples of the present invention. Skin epidermal keratinocytes were injected into a 96 well microtiter plate so that the number of cells per well was 5 X 10 ³ cells/100 μL/well, and cultured in a 5% CO ₂ incubator at 37°C. Thereafter, the essential oil derived from Matari flowers was treated with 100 μL in each well at a concentration of 0 to 250 μg/mL and incubated for 24 hours. Ez-cytox was treated with 10 μL per well and reacted for 30 minutes in an incubator, and then absorbance was measured at 450 nm using an ELISA plate reader (Synerge 2, BioTek, USA). The measurement results were converted into percentages to calculate the survival rate of skin epidermal keratinocytes of essential oil derived from Matari flowers.

As a result, no significant cytotoxicity was observed in the essential oil derived from Matari flowers at a concentration of 1 to 250 μg/mL (see FIG. 3).

Example 4. Effect of Matari Flower Essential Oil on Improving Epidermal Keratinocyte Proliferation

The effect of the essential oil derived from Matari flowers obtained in Example 1 (absolute) on the proliferation of epidermal keratinocytes was confirmed. Specifically, human skin-derived epidermal keratinocytes (HaCat) were cultured in DMEM medium containing 10% fetal bovine serum, and used in experimental examples of the present invention. Skin epidermal keratinocytes were injected into a 96 well microtiter plate so that the number of cells per well was 3 X 10 ³ cells/100 μL/well, and cultured in a 37°C, 5% CO ₂ incubator. Thereafter, 100 μL of matari flower absolute was treated at a concentration of 0 to 200 μg/mL in each well, and incubated for 48 hours. Here, the negative control group is the untreated group without treatment with Matari flower absolute, and the positive control group is the epithermal growth factor (EGF 50 ng/mL), which is conventionally used in skin regeneration and/or wound healing agents. I did.

Thereafter, 10 μL of Ez-cytox was treated in each well and then reacted for 30 minutes in an incubator. After measuring the absorbance at 450 nm using an ELISA plate reader (Synerge2, BioTek, USA), the result of the measurement was converted into a percentage to confirm the result of the proliferation rate of skin epidermal keratinocytes of essential oil derived from Matari flowers.

4 is a graph showing the induction rate of skin epidermal keratinocyte proliferation of essential oils derived from Matari flowers extracted according to Example 1 of the present invention,

As shown in Figure 4, it was found that the essential oil derived from Matari flowers according to the present invention induces the proliferation of skin epidermal keratinocytes in a concentration-dependent manner.

Example 5. Effect of Matari Flower Essential Oil on Improving Skin Epidermal Keratinocyte Migration

In order to confirm the effect of the essential oil derived from Matari flowers obtained in Example 1 on the migration of epidermal keratinocytes, the concentration of the essential oil derived from Matari flowers suggested in Example 2 was investigated as follows.

Specifically, human-derived epidermal keratinocytes (HaCat) were cultured using DMEM medium containing 10% fetal bovine serum and used in the experiment of the present invention. The migration rate of epidermal keratinocytes was investigated using a 48 well chemotactic boyden chamber. First, the essential oil derived from Matari flowers was treated at a concentration of 1 to 200 μg/ml, respectively, and an 8 μm pore poly-carbonate membrane coated with collagen was put on the lower chamber. Then, the epidermal keratinocytes were treated in the upper chamber at 5×10 ⁴ cells/50 μl per well. After incubating the Chamber in an incubator at 37°C for 210 minutes, the membrane is stained and fixed with Diff-Quick solution, and the number of cells that have passed through the membrane is counted and converted into a percentage. The results were obtained.

5 is a graph showing the migration effect of the skin epidermal keratinocytes of the essential oil derived from Matari flowers extracted according to Example 1 of the present invention, the negative control was not using the essential oil derived from Matari flowers, the positive control was conventional It uses an epithermal growth factor that is used for wound healing and/or skin regeneration.

As a result, as shown in FIG. 5, it was confirmed that the essential oil derived from Matari flowers induces the migration of skin epidermal keratinocytes in a concentration-dependent manner, and in particular, exhibits a maximum migration rate of 214.18±7.40% at a concentration of 150 μg/ml. , Has been shown to induce movement very strongly.

Example 6. Effect of Matari flower essential oil on enhancing phosphorylation of signaling factors in epidermal keratinocytes

The essential oil derived from Matari flowers obtained in Example 1 above is the main signaling process that regulates the proliferation and migration of epidermal keratinocytes, of extracellular signal regulation kinase (ERK1/2) and intracellular serine/threonine protein kinase (Akt). In order to measure the effect on the phosphorylation process, the concentration of the essential oil derived from Matari flowers suggested in Example 2 was treated, and each specific antibody (P-ERK1/2, ERK1/2, P-Akt, Akt,) was used. Thus, the immunoblot (Immuno Blot) method was used.

Figure 6 (A) is the phosphorylation process of epidermal keratinization extracellular signal-regulating kinase (ERK1/2) and intracellular serine/threonine protein kinase (Akt) of the essential oil derived from Matari flowers extracted by Example 1 of the present invention. This is a result showing the effect on, and the negative control was not using the essential oil derived from Matari flowers, and the positive control was the epithermal growth factor (EGF 5 ng/mL) used for conventional wound healing and/or skin regeneration. ).

In addition, as shown in (B) and (C) of Fig. 6, the essential oil derived from Matari flowers is a concentration-dependently of epidermal keratinocyte extracellular signal regulation kinase (ERK1/2) and intracellular serine/threonine protein kinase (Akt). Phosphorylation was induced.

Example 7. Effect of promoting out growth of collagen sprouting of Matari flower essential oil

In order to measure the effect of the essential oil derived from Matari flowers obtained in Example 1 on the out growth of epidermal keratinocyte collagen sprouting, it was investigated as follows. Specifically, after mixing Type I collagen and epidermal keratinocytes to a concentration of 2.5×10 ⁷ cells/mL, neutralizing with NaOH (1 N) solution, spotting 5 μL each on a 24 well plate, and 5 to 10 It dried at room temperature for about a minute to form an artificial skin tissue. The samples were processed and incubated in a 37° C., 5% CO ₂ incubator for 72 to 90 hours. The culture medium was removed, cells and tissues were fixed and stained using Diff-Quik solution, and the stained cells and tissues were photographed to obtain the results.

Figure 7 is a result showing the effect on the growth (out growth) of the epidermal keratinocyte collagen sprout (sprouting) of the essential oil derived from Matari flowers extracted by Example 1 of the present invention, the negative control is using the essential oil derived from Matari flowers It was not, and the positive control was the use of epithermal growth factor (EGF 50 ng/mL) that is used for conventional wound healing and/or skin regeneration.

As a result, as shown in Fig. 7 (A), it was found that the essential oil derived from Matari flowers induces out growth of collagen sprouts of epidermal keratinocytes in a concentration dependent manner. That is, compared with the positive control group, it was found to effectively induce out growth of keratinocyte collagen sprouting at a concentration of 150 μg/mL of Matari flower essential oil.

So far, the present invention has been looked at around its preferred embodiments.

Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the above description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (12)

In a composition for external application for skin for skin regeneration or wrinkle improvement, comprising essential oil extracted from Matari flower (Patrinia scabiosaefolia flower) at a concentration of 50 to 200 μg/mL,
The essential oil is a first step of immersing the Matari flowers in an organic solvent; A second step of evaporating and concentrating the immersion liquid of step 1 to obtain concrete; A third step of removing the insoluble wax by dissolving and filtering the concrete of step 2 in an organic solvent; And After removing the insoluble wax in the third step, essential oils prepared in four steps of separating and purifying the absolute, skin external composition for skin regeneration or wrinkle improvement. delete delete The method of claim 1,
Essential oils extracted from the Matari flowers are Ursa-9(11),12-dien-3-one, Ursa-9(11),12-dien-3-ol, beta-sitosterol, and aristolone ( Aristolone), alpha-amyrin (α-Amyrin) comprising any one or more components selected from the group consisting of, skin regeneration or wrinkle improvement composition for external application for skin. delete The method of claim 1,
The composition is to induce proliferation and migration of epidermal keratinocytes, skin regeneration or wrinkle improvement composition for external application. The method of claim 1,
The composition induces one or more phosphorylation processes or signaling processes selected from the group consisting of epidermal keratinocyte extracellular signal regulation kinase (ERK1/2) and intracellular serine/threonine kinase (Akt), skin for skin regeneration or wrinkle improvement Composition for external application. The method of claim 1,
The composition has an effect of promoting the growth of epidermal keratinocyte collagen sprout, skin regeneration or wrinkle improvement composition for external application. In a cosmetic composition for skin regeneration or wrinkle improvement, comprising essential oil extracted from Matari flower (Patrinia scabiosaefolia flower) at a concentration of 50 to 200 μg/mL,
The essential oil is a first step of immersing the Matari flowers in an organic solvent; A second step of evaporating and concentrating the immersion liquid of step 1 to obtain concrete; A third step of removing the insoluble wax by dissolving and filtering the concrete of step 2 in an organic solvent; And After removing the insoluble wax in the third step, essential oils prepared in four steps of separating and purifying the absolute, a cosmetic composition for skin regeneration or wrinkle improvement. The method of claim 9,
Essential oils extracted from the Matari flowers are Ursa-9(11),12-dien-3-one, Ursa-9(11),12-dien-3-ol, beta-sitosterol, and aristolone ( Aristolone), alpha-amyrin (α-Amyrin), comprising any one or more components selected from the group consisting of, skin regeneration or wrinkle improvement cosmetic composition. delete delete

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