KR101516063B1 - Skin moisturizing composition comprising Platycodin grandiflorum containing platycodin D - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing comprising an extract of Platycodin D (Platycodin D) as an active ingredient. Platycodin D according to the present invention is excellent in moisturizing power of the skin , And can be used as a cosmetic composition for moisturizing the skin, which keeps moisture constantly.

Description

[0001] The present invention relates to a skin moisturizing composition comprising Platycodin grandiflorum containing platycodin D as an active ingredient,

The present invention relates to a cosmetic composition for skin moisturizing comprising Platycodin D-containing bellflower extract as an active ingredient.

Skin is divided into three layers, epidermis, dermis and subcutaneous fat tissue in order from the outside. When we look at the structure of skin, outer epidermis is functionally divided from outside to inside in stratum corneum, transparent layer, granular layer and basal layer , The inner dermis (dermis) is a tight connective tissue, and hypodermis inside the dermis (hypodermis) to fix the skin to the internal organs. In addition, the skin has about 65 ~ 70% of the human body's moisture, which plays an important role in helping the skin maintain its soft and moist condition.

It has been reported that the skin layer of the epidermis must have an essential moisture content of 10% or more, and the thickness of the stratum corneum varies depending on the content.

The cells that make up this stratum corneum have a high concentration of Natural Moisturizing Factor (NMF), which is a water-soluble component, which helps the skin to maintain flexibility and maintain proper moisture. For example, The material is not only water-soluble, but also effectively combines with moisture to inhibit moisture from drying out of the skin.

Platycodon grandiflorum is a perennial plant that is a perennial plant with a height of about 1 m. It has no hairs, and its root is large, with one stem gathering or gathering and growing straight.

Flowers bloom in July or August in light blue or white, and bell-shaped flowers are a flower orchid with one petal on the tip of a stem or on the tip of a cracked branch and the flower corolla is made up of five petals. Calyxes are bell-shaped and are divided into 5 pieces. Each piece is triangular barcelial (lanceolate), and there are 5 stamens. The head of the stigma is divided into 5 branches and the lower ovary.

In the one room, the part made by removing the root or juniper is used as medicine and it is wild in mountains and fields, and it is distributed in Korea, Japan, China and so on.

The main pharmacological component of the bellflower is triterpenoid saponin. It has the effect of eliminating the phlegm by increasing the bronchial secretion. The saponin component which is observed only in the bellflower is calm, fever, pain, calmness, , Improvement of cholesterol metabolism, anticholinergic, anticancer effect and inhibition of gastric acid secretion.

The major cause of skin aging is known to be influenced by various factors such as the content of various polysaccharides in the skin and the immune action ability to the external environment. One of the major influences is skin moisture content have.

Conventionally, a method of increasing water retention has been widely used by using a humectant having a property of absorbing moisture as a humectant and an occlusive moisturizer preventing evaporation of water. In addition, the formulations containing the lamellar liquid crystals, which have been undergoing much research until recently, are known to have excellent moisture retention due to their similar merits to the keratinous structures of the skin, but they can be manufactured only in a specific composition of the raw materials and thus have problems in universal use.

As a conventional technical field corresponding to the present invention, technologies featuring skin moisturizing in the cosmetics industry have been published through numerous papers and patents, and have been industrialized into a wide variety of products.

In general, a skin moisturizing cosmetic material such as a skin moisturizing cosmetic using a substance obtained by extracting various plants such as cucumber extract, carrot extract, green tea extract, safflower seed extract, mashin extract, ginkgo biloba extract, Lotions, creams, essences, and packs.

Studies using platycodon extracts have been extensively studied, but research on cosmetics and skin moisturizers containing platycodin D-containing platycodon extracts has hardly been found. However, according to Korean Patent Registration No. 10-0564927, there is a patent for an anticancer agent containing platycodine as an active ingredient, and in accordance with Korean Patent Registration No. 10-0750333, there is provided a method for preventing hypercholesterolemia comprising platycodin D, There is a patent relating to a therapeutic agent, and according to Korean Patent Application No. 10-2012-0087125, there is a known activity for a composition for treating allergy comprising platycodin D-containing bellflower extract.

The present invention relates to a novel antiallergic composition having excellent antiallergic, anticancer and antiinflammatory activity, and a composition for prevention or treatment, which are suitable for foods, medicines and cosmetics, The study on the moisturizing effect of Platycodin D-containing bellflower extract is not known.

The moisture of the stratum corneum is decreased due to lifestyle factors such as cleansing and bathing, seasonal factors and pollutants, etc., and the skin becomes dry, the surface becomes rough, the skin becomes dull and the skin looks dull The problem concerning the skin moisturizing agent is getting larger and larger.

Various cosmetic raw materials are being developed in order to improve skin moisturization and elasticity degradation. As the importance of environmentally friendly products is increasing, there is a growing demand for safety of cosmetics for consumers. As a result, organic natural cosmetics are in the spotlight and demand for environmentally friendly cosmetics is expected to increase significantly regardless of price regardless of age and sex.

Accordingly, the present inventors have completed the present invention by confirming the skin moisturizing effect which can prevent the cause such as roughness of the skin, decrease in the skin luster and appearance of the fine lines as platycodin D-containing bellflower extract Respectively.

And a cosmetic composition for moisturizing the skin comprising Platycodin D-containing bellflower extract as an active ingredient.

In order to achieve the above object, the present invention is a natural cosmetic product capable of soothing the skin from various external factors, and is intended to alleviate the roughness of the skin, the decrease in the skin luster and the appearance of the appearance of fine lines, And a platycodin D-containing bellflower extract which is safe and maintains moisture continuously as an active ingredient.

It can be used as a cosmetic composition for skin moisturizing which is excellent in skin moisturizing power, containing platycodin D-containing bellflower extract according to the present invention as an active ingredient, safe, and maintains moisture continuously.

1 is a graph showing the skin moisturizing effect of a cream type cosmetic preparation containing platycodin D-containing bellflower extract according to the present invention measured by MY-808S Moisture Checker (Scalar, Japan).
FIG. 2 is a graph showing the skin moisturizing effect of a serum type cosmetic preparation containing platycodin D-containing bellflower extract according to the present invention measured by MY-808S Moisture Checker (Scalar, Japan).

The present invention provides a cosmetic composition for skin moisturizing comprising, as an active ingredient, Platycodin D-containing bellflower extract represented by the following formula (1):

[Chemical Formula 1]

.

.

Platycodin D is a kind of flavanone, and its IUPAC name is 3? - (? - D-glucopyranosyloxy) -2 ?, 16 ?, 23,24-tetrahydroxy-OD- -D-furanosyl- (1 → 3) -O- β-D-xylopyranosyl- (1 → 4) -O-6-deoxy-olean-12-en-28-oic acid. Molecular Formula ) Is C ₅₇ H ₉₂ O ₂₈ and the molecular weight is 1225.32.

In one embodiment according to the present invention, the bellflower extract powder containing Platycodin D is 0.00001 to 1 wt% of the total weight of the cosmetic composition.

In one embodiment according to the present invention, the bellflower extract powder containing Platycodin D is 0.0001 to 0.1% by weight of the total weight of the cosmetic composition.

In one embodiment according to the present invention, the bellflower extract powder containing Platycodin D is 0.001 to 0.01 wt% of the total weight of the cosmetic composition.

In one embodiment of the present invention, Platycodin D-containing bellflower extract is prepared by extracting bellflower with water or a lower alcohol having 1 to 4 carbon atoms, fermenting it, centrifuging it to remove solid components, concentrating, lyophilizing The present invention relates to a cosmetic composition for skin moisturizing.

The cosmetic composition for skin moisturizing of the present invention has no toxicity because it is an edible natural product extract or platycodine D isolated therefrom as an active ingredient and is harmless to human body.

In one embodiment according to the present invention, the composition of the cosmetic composition may be in the form of a skin, lotion, cream, essence, emulsion, gel, lipstick, cleansing foam, cleansing cream, cleansing water, spray, shampoo, rinse, , A pack, a massager, a face powder, a compact, a foundation, a two-way cake, and a makeup base.

In one embodiment according to the present invention, a method of moisturizing a skin that requires moisturization, comprising administering a cosmetic composition according to claim 1 on a skin surface.

In one embodiment according to the present invention, a method is provided wherein the skin requiring moisturizing is dry skin.

In one embodiment according to the present invention, the method is wherein the skin requiring moisturizing is a mild to moderate skin.

The skin moisturizing effect, which keeps the cracked skin such as dry skin, weakly dry and moderate dryness always in a soft and moist state, is remarkably improved, and acts to remove toxins from contaminated or damaged skin, There is a cleansing action effect that suppresses the occurrence of wrinkles and eliminates skin troubles and side effects.

In one embodiment according to the present invention, the step of extracting Platycodon grandiflorum with water or a lower alcohol having 1 to 4 carbon atoms and fermenting it, followed by centrifuging to remove solid matter; Concentrating, and lyophilizing the skin of the plant.

In one embodiment according to the present invention, the method is for producing a platycodine D-containing bellflower extract for skin moisturizing, wherein the lower alcohol is methanol or ethanol.

Hereinafter, the present invention will be described in more detail with reference to the following Examples, Experimental Examples and Preparation Examples. It should be understood, however, that the same is by way of illustration and example only and is not intended to limit the scope of the present invention.

[Example 1] Method for producing platycodin D-containing bellflower extract

Standardized 1% by weight, 3% by weight, 5% by weight platycodin D-containing bellflower extract was supplied from BN C Co., Ltd., and specific preparation was as follows.

The raw material of the bellflower was extracted more than twice with water (purified water), ethanol or methanol, and the obtained extract was centrifuged after fermentation to remove solid components, followed by concentration and lyophilization to obtain respective extracts from which the solvent was removed. In order to separate and purify the single active component from the extract, the extract obtained above was completely dissolved in water, and the activity of each fraction was confirmed using RP-18, Diaion HP-20, and ion exchange gel column Only the visible fractions were obtained, and each single active component was isolated and purified by further HPLC.

 ○ Analysis equipment

- HPLC system: Agilent 1260 series

Column: Kinetex C ₁₈ column (2.6 占 퐉, 4.6 mm 占 100 mm, Phenomenex, Germany)

- Detector: Alltech ELSD 2000

○ HPLC and ELSD conditions

Samples were analyzed using Agilent 1260 series HPLC.

The column used was 0.15% formic acid (A), acetonitrile (B), and methanol (C) as the mobile phase, and the following conditions were used for the column: C ₁₈ column (2.6 ㎛, 4.6 mm x 100 mm, Phenomenex, Germany) And the platycodine D, which is a target substance, was analyzed by a gradient flow method.

After completion of the slurry solvent elution, post-run was performed with 0.15% formic acid (A): acetonitrile (B): methanol (C) = 75: 20: 5 (v / v) for 10 minutes to stabilize the column. At this time, the injection amount and the flow rate of the sample were adjusted to 20 μl and 0.6 ml / min.

Platycodin D samples were detected by ELSD detector. Platycodin D was quantified by setting the flow rate and temperature at 1.5 L / min and 80 ° C using nitrogen as the drying gas.

○ Standard quantitative curve of platycodine D standard

Platycodin D was accurately weighed and dissolved in 50% methanol, and then platycodin D content was determined using a standard quantitative curve using HPLC / ELSD. Calibration curve of the standard quantitative curve obtained from the standard product of platycodine D showed good linearity at y = 17.757x-643.76 (R ² = 0.999).

For standardization of the test materials, each sample was dissolved in 50% methanol to a concentration of 20 mg / ml, and impurities were removed (0.45 μm filter) and HPLC / ELSD detection was performed.

From the chromatogram obtained, the peak area ratio of platidone D to the peak area of the internal standard material was determined, and the concentration of platycodin D in the extract was determined from a previously prepared standard curve to normalize the product.

Experimental Example 1 Skin moisturizing effect test of Platycodin D-containing bellflower extract

A cream and serum containing 0.008% of powder of Platycodin D-containing bellflower extract were made to make a skin moisturizing effect experiment. MY-808S Moisture Checker (Scalar, Japan) was used for the skin moisturizing effect and clinical examination was performed using the inside of the forearm.

In the method of measuring the moisture resistance, the skin moisturizing power before application was checked including the control group, and the skin moisturizing power after 1 hour, 2 hours, 3 hours, and 4 hours after each sample was applied at 0.25 g / cm ² was measured. The skin moisturizing ability was very sensitive to temperature and humidity, so it was conducted in a constant temperature and humidity room where the temperature was 20 and the humidity was 60%.

The subjects were tested in 20 male and 5 female subjects.

[Results of skin moisturizing test using MY-808S Moisture Checker]

First, the results of the test with the MY-808S Moisture Checker (MY-808S Moisture Checker) are shown in FIG. As shown in FIG. 1, the skin moisturizing effect was increased from 30.88% of the skin moisture measured before applying to the cream containing 0.008% of platycodamine D containing powder, to 38.02% of skin moisture measured after application, The skin moisturizing effect with time elapsed gradually after 4 hours, but it was higher than that of normal skin condition.

Also, as shown in FIG. 2, in the serum containing 0.008% of Platycodin D-containing extract powder, skin moisturizing effect was increased from 30.08% of the skin moisture before application to 37.96% of skin moisture content after application , And the skin moisturizing effect with time elapsed gradually after 4 hours, after the skin moisturizing effect was applied, but it was higher than that of the normal skin condition. The reason is that the platycodamine D-containing bellflower extract covers the surface of the stratum corneum and prevents moisture loss of the skin, and it is interpreted that the moisturizing effect is maintained due to the holding effect of the skin moisture.

On the other hand, as shown in FIG. 1 and FIG. 2, the serum containing 0.008% of platycodine D-containing bellflower extract powder had a higher moisturizing effect than the cream containing 0.008% of platycodamine D-containing bellflower extract powder.

 Production Example 1. Skin moisturizing skin preparation

These ingredients were used to make the skin according to the conventional method in the field of cosmetics for making a skin.

The above ingredients were used to prepare according to the conventional method in the field of cosmetic production for the production of serum.

The above ingredients were used to prepare according to a conventional method in the field of cosmetics for the manufacture of lotions

The above ingredients were used to prepare according to the conventional method in the field of cosmetic preparation for essence production

These ingredients were used to prepare the cream according to the conventional method in the field of cosmetic production.

The ingredients were prepared according to the conventional method in the field of cosmetic production for pack manufacture.

The above ingredients were used to prepare the cleansing lotion according to the conventional method in the field of cosmetic production.

These ingredients were used to make the makeup base according to the conventional method in the field of cosmetic production.

The above ingredients were used to make powders according to the conventional method in the field of cosmetic production for powder facts production.

The ingredients were used to make the usual methods in the field of cosmetics for the manufacture of two-way cakes.

Claims (11)

Which comprises as an active ingredient, a bellflower extract containing Platycodin D represented by the following formula (1)
0.00001 to 0.1 wt% of the total weight of the cosmetic,
Herein, Platycodin D-containing bellflower extract is prepared by extracting Platycodon grandiflorum with water or a lower alcohol having 1 to 4 carbon atoms, fermenting it, centrifuging it to remove solid content, and concentrating and lyophilizing it Cosmetic composition for skin moisturizing:
[Chemical Formula 1]

.
delete delete The method according to claim 1,
The platycodin D-containing platycodextrin powder is 0.001 to 0.01 wt% of the total weight of the cosmetic composition.
delete The method according to claim 1,
The cosmetic composition may be in the form of a skin, lotion, cream, essence, lotion, gel, lipstick, cleansing foam, cleansing cream, cleansing water, spray, shampoo, rinse, , A foundation, a two-way cake, and a make-up base.
A method for moisturizing skin requiring moisturization comprising administering a cosmetic composition according to claim 1 on a skin surface.
8. The method of claim 7,
Wherein the skin requiring moisturizing is dry skin.
8. The method of claim 7,
Wherein the skin requiring moisturizing is a mild to moderate skin.
delete delete

Images