KR100887294B1 - Cosmetic compositions containing hollyhock extracts - Google Patents

Abstract

The present invention is a cosmetic composition containing the extract of the root muscle, the extract of the root of the root and the extract of 5 to 30 times the volume of the extraction solvent with respect to the dried root of the root muscle, and the extractor 4 to 48 at 30 to 70 ℃ Extraction by heating for a time, or may be prepared by depositing the mucus component by depositing the mycoccal muscle at 5 to 40 ℃ for 1 to 15 days and then precipitated in a solvent. The mucus extracted and isolated from the muscles and products containing it have moisturizing, cell proliferative effect, anti-inflammatory, itching suppression, and anti-atopic effect, which enhances and prevents various inflammatory problems and diseases of the skin to strengthen skin barrier function. It can be mix | blended and used for various cosmetic compositions.

Tectonic muscles, moisturizing, anti-inflammatory, skin improvement, cosmetic composition

Description

Cosmetic composition containing kokkukeun extract {Cosmetic compositions containing hollyhock extracts}

Figure 1 shows the weight average molecular weight of the chokgyu muscle extract according to an embodiment of the present invention.

Figure 2 shows the IR spectrum of the chokgyukeun extract according to an embodiment of the present invention.

Figure 3 is a graph showing the inhibition rate of foot edema by each sample according to an embodiment of the present invention.

Figure 4 is a graph showing the inhibition rate of foot edema by the applied product according to an embodiment of the present invention.

5 is a graph showing the foot edema suppression effect according to an embodiment of the present invention.

6 is a graph showing an atopy improvement effect according to an embodiment of the present invention.

Figure 7 shows the clinical picture of the atopy improvement effect when using the chokkukeun extract according to an embodiment of the present invention.

8 is a graph showing the satisfaction results for the skin improvement effect according to an embodiment of the present invention.

Figure 9 is a graph showing the water evaporation rate at 15% relative humidity conditions for each sample according to an embodiment of the present invention.

10 is a graph showing the water evaporation rate at 55% relative humidity for each sample according to an embodiment of the present invention.

11 is a graph showing the water evaporation rate at 15% relative humidity for each applied product according to an embodiment of the present invention.

12 is a graph showing the water evaporation rate at 55% relative humidity for each applied product according to an embodiment of the present invention.

13 is a graph showing the increase rate of the moisturizing power for each sample according to an embodiment of the present invention.

Figure 14 is a graph showing the increase in moisturizing power for each applied product according to an embodiment of the present invention.

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cosmetic composition comprising an extract of Chok-Gyu-Keun, and more particularly to a cosmetic composition containing mucus obtained by extracting Chok-Gyu-Keun as a moisturizing and anti-inflammatory component.

The outermost layer of skin, the stratum corneum, has a barrier against moisture evaporation and absorption of the skin and the penetration of external substances such as chemicals, toxic substances and bacteria. In 1969 Schreiner and Wolff found that the stratum corneum acts as a barrier to the epidermis. Lipids in the stratum corneum are mainly present in the intercellular space, and they are derived from lamellar bodies, and these small membranous discoid structures are fused to form intercellular lamellar structures. Through this membrane fusion process, a wide and non-fatty fat layer is formed, which acts as a barrier to water evaporation. The intercellular lipids alone cannot act as a barrier to water loss, and the keratinocytes with a large fat double layer The correct combination of is essential. The monomolecular layer of the fat layer located on top of keratinocytes and the protein membrane layer of keratinocytes are covalently linked to each other to achieve this exact bonding.

The stratum corneum is filled with flat corneocytes and intercelluler lipids between them, forming a lamellar form. The stratum corneum of the skin contains about 20% water, and it is well known that the moisture content is an important factor in determining the flexibility and moisture of the skin. Epidermal lipids correspond to 10-30% of the total volume of the stratum corneum. There are 100 to 200 times more material filling between cells than in other tissues. These are intercellular lipids that hold water. The stratum corneum is an effective permeable barrier and has two important functions. First, the stratum corneum prevents the invasion of microorganisms, chemicals and allergens. It also minimizes transepidermal water loss (TEWL) so that our bodies are not dehydrated. When the stratum corneum is removed, the skin's moisture is better out with the epidermal lipids (increasing TEWL), and other substances, such as toxins and allergens, can easily enter. Due to this, the stratum corneum of the skin is damaged, and the protective barrier function decreases gradually, and various inflammatory reactions such as increased water loss, itching, erythema, edema, allergy, contact dermatitis, and sensory dermatitis occur.

The ability to store water in the skin depends largely on the lipid barrier of the stratum corneum. The keratin protein structure of keratinocytes contains natural moisturizing factors (NMF), such as amino acids, and affects the skin's ability to bind water.

To maintain 30% normal moisture in the stratum corneum, the following three factors must be maintained. ① Sebum ② Natural moisturizing factor ③ If any one of intercellular lipids, sebum, NMF, and intercellular lipids is lowered, that is, if they do not have a healthy keratin cycle, the skin's ability to hold water also dries up and the skin dries and dehydrates. In the external preparations for skin, the development of safe, high usability and high moisturizing effect like natural moisturizing factor is one of the important research tasks in cosmetics. Recently, it has been found that the factors influencing the function of the stratum corneum (transdermal absorption, water retention, dropping of the stratum corneum) are keratinous lipids.

As the skin ages, its skin gradually deteriorates, and aging occurs. Typical examples are wrinkle formation and melanin production. The main cause of such aging is photoaging due to sunlight. The free radicals generated by the ultraviolet rays of sunlight stimulate the oxidative cleavage of collagen and elastin, the connective tissue of the skin, and consequently cause wrinkles by abnormal crosslinking of connective tissue. In addition, it promotes the cleavage and low molecular weight of hyaluronic acid, which plays a major role in the skin's water retention ability, thereby reducing the water retention capacity of the stratum corneum. In general, when the moisture of the stratum corneum is 10% or less, the physiological action of the skin is lowered, the skin loses its original function, becomes rough, and causes various skin problems.

Currently used moisturizers include polyols such as glycerin, propylene glycol and butylene glycol, natural moisturizing factors such as amino acids, pyrrolidone carbonates and lactates, biopolymers such as hyaluronic acid salts, chondroitin sulfates and chitosan, etc. There is this. Some of these are moisturizers that, although exhibiting excellent moisturizing properties, are synthetic compounds or animal-derived moisturizers, which, when used in excess, can not exclude skin irritation or contamination-induced infections. In addition, a variety of vegetable moisturizers have been developed, but the bark or root bark of the tree in general has a limitation of mass production due to the time required to secure the material and the problems associated with the purchase. In addition, the material used as an anti-inflammatory agent having an effect of improving skin inflammation is a drug or a synthetic substance such as indomethacin, and thus its usage is limited, and it is difficult to use because of high cost.

Therefore, it does not have cytotoxicity and skin irritation, is easy to cultivate and extract, and has excellent skin barrier function such as moisturizing and anti-inflammatory, anti-itch, itching and atopic improvement of skin, and it has excellent compatibility with cosmetic raw materials and contains the material. We need development of cosmetics to say.

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic composition containing mucus obtained by extracting and separating from a muscle muscle as a moisturizer and having no skin irritation and having excellent moisturizing power.

In order to achieve the above object, the present invention

Provided is a cosmetic composition containing a choksu muscle extract.

The present invention relates to a cosmetic composition extracted, separated, and purified from the muscle root, and after drying the muscle root, 5-30 times the volume of the extraction solvent is added to the dried muscle root, and the extractor is 4 to 48 at 30 to 70 ℃. It can be prepared by heating and extracting for a time. In addition, the extract may be prepared by depositing the mucus to extract the mucus component for 1 to 15 days at 5 to 40 ℃ to precipitate in a solvent.

One or more solvents selected from the group consisting of water, phosphate buffer solution, sodium carbonate aqueous solution, glycerin, ethylene glycol, propylene glycol, and 1,3-butylene glycol may be used to extract the mucus component.

The inventors of the present invention searched for a variety of natural substances in order to find a substance having excellent skin moisturizing power, it was confirmed that the mucus material extracted and separated from Tsukyu muscle (dish root) shows very good skin moisturizing power. It has no safety problems as a raw material used as a plant and used as a herbal medicine as a conventional food, and also has the effect of maintaining the skin's moisturizing power for a certain period of time even in the results of experiments applied directly to human skin by combining a certain concentration in the cosmetic composition. I found it to work out.

Chok Kyu-Geun is the root of the mallow family Althaea rosea CAV. It is also known as Tak-Gyu, Duk-Du-Jung, Jun-Jung-Kyu, Tak-Gyu, Tak-O-Gum. It is native to China and grows on roadside glade. The root is called Kyu-Kyu-Keun, and the flower is called Kyu-Kyu-hwa. Ornamental and medicinal for leaves, stems and roots. The root contains a large amount of mucus, and the mucus contains pentose, pentosan, methyl pentosan, and uronic acid. There are two kinds of plants of the family, Hwang Chok-gyu, aka Dakmul, and have similar effect to Chok-gyu. According to Chinese doctors, Chok Keun-Geun was used to lower heat, remove inflammation, and treat swelling. Also, in the domestic civilian use roots and flowers diarrhea, diuretic and other drugs. (Complementary Chinese Medicine Dictionary, Chinese Medicine Dictionary Committee, Book Publishing Confidence, Ingredients and Utilization of Herbs, Science Encyclopedia, Encyclopaedia, Ilwol Seomyeon, Therapeutic Medicine Illustrated for Herbal Medicine Foundation, Information Sub, Shin Mingyo, Medicinal Plant, P. 561)

The cosmetic composition of the present invention can obtain a safe and moisturizing vegetable mucus that is not toxic to the stratum corneum from the skin muscle, and improves the skin moisturizing ability using the vegetable mucus and at the same time skin inflammation, itching, atopic skin, etc. Trouble can be reduced.

The present invention relates to a mucus extracted and separated from the muscle muscle, the manufacturing method of extracting and separating the mucus from the muscle muscle is as follows.

First, the extract is dried and then 5 to 30 times the volume of the extraction solvent is added to the dried root. The extraction solvent uses at least one solvent selected from the group consisting of water, phosphate buffer, sodium carbonate aqueous solution, glycerin, ethylene glycol and propylene glycol. Subsequently, in order to prevent the solvent from evaporating, extraction is carried out by heating at 30 to 70 ° C. for 4 to 48 hours in an extractor equipped with a cooling condenser or by dipping at 5 to 37 ° C. for 1 to 15 days, followed by methanol and ethanol. It is prepared by precipitation in one or more solvents selected from the group consisting of, propanol, butanol and acetone. The mucus prepared in this way is contained as a moisturizing and anti-inflammatory effect component.

In the cosmetic composition of the present invention, the mucus is added in an amount of 0.01 to 10% by weight, preferably 0.1 to 5% by weight as dry weight.

Cosmetics containing the mucus of the present invention have a formulation of conventional basic cosmetics such as soft lotion, nourishing lotion, nourishing cream, massage cream, essence and pack. Excipients, thickeners and the like may be used to prepare the formulations.

Examples, prescription examples and comparative examples below describe the contents of the present invention, but the content of the present invention is not limited thereto, and a cosmetic composition other than the following examples and prescription examples may be prepared by containing general cosmetic components. Can be.

Example

Example 1

After washing the pickled roots (dish flower roots) well without any foreign substances, semi-dry them in a well-ventilated shade, and add 10kg of purified water to 1kg of finely cut roots, and use an extractor for 24 hours at 40 ~ 50 ℃ bath water. After extraction, the filtrate was filtered through a 400 mesh filter cloth, and the filtrate was centrifuged at 3000 rpm. The supernatant from which impurities were removed was taken and lyophilized to obtain 220 g of solid content. 1 kg of ethanol was added to 220 g of the solid, and the mixture was slowly stirred to remove substances and pigments other than the mucus component present in the solid, followed by filtration and drying with a 1 µm filter to obtain 182.7 g of solid.

The solids were analyzed using a MALDI-TOF device (Perseptive Biosystems, Voyager RP) and FT-IR (Jasco, FT / IR 410) to analyze the weight average molecular weight and the function group of solids.

It was 4800 when the weight average molecular weight was confirmed by MALDI-TOF. The conditions of the analysis are as follows. Mode is Linear, Scans averaged is 47, Gride Voltage is 99.00%, Low Mass Gate is 800, Guide wire voltage is 0.25%, Pressure is 2.08 e-07, Negative Ions is OFF, and FT-IR is resolution 4cm-1, Codine was used for apodization and 2mm / sec for scan speed. The results are shown in FIGS. 1 and 2.

Example 2

After washing the pickled roots (dish flower roots) well without foreign substances, semi-dry them in a well-ventilated shade, and then put 10kg of purified water into 1kg of finely cut tentacles, immersion extraction at room temperature for 3 days, and filter them with 400 mesh filter cloth. After the filtrate was centrifuged at 3000rpm, the supernatant from which impurities were removed was taken and lyophilized to obtain 235g solid. 1 kg of ethanol was added to 235 g of the solid, and the mixture was slowly stirred to remove substances and pigments other than the mucus component present in the solid, followed by filtration and drying with a 1 µm filter to obtain 184.9 g of solid.

Example 3

After washing the pickled roots (dish flower roots) well without any foreign substances, semi-dry them in a well-ventilated shade, and add 10kg of purified water to 1kg of finely cut roots, and use an extractor for 24 hours at 40 ~ 50 ℃ bath water. After extraction, the filtrate was filtered through a 400 mesh filter cloth, precipitated in 15 kg of ethanol, filtered, and dried to obtain 143 g of solid content. 12 kg of purified water was added to 143 g of this solid, and after removing particulate impurities using a diatomaceous earth filter and a glass filter, an aqueous solution of 10 kg was obtained. And precipitated in 15kg of ethanol to obtain 110g of solid content.

Example 4

After washing the dried Cucumber roots (Dish Root) well without any foreign matters, semi-dry them in a well-ventilated shade, and add 10 kg of purified water to 1 kg of fine Cucumber roots, immersion extraction at room temperature for 3 days, and filter with 400 mesh filter cloth. Precipitated, filtered and dried in 15 kg of ethanol to obtain 150 g of solid content. 15 kg of purified water was added to 150 g of this solid, and after removing particulate impurities using a diatomaceous earth filter and a glass filter, an aqueous solution of 12.1 kg was obtained. And precipitated in 15kg of ethanol to obtain a solid content of 118g.

Example 5

After washing the picked roots (dish root) well without any foreign matters, semi-dry them in a well-ventilated shade, and add 10kg of purified water to 1kg of fine roots, and adjust the pH to 8 using an aqueous solution of sodium carbonate. The mixture was extracted at room temperature for 3 days, filtered through a 400 mesh filter cloth, precipitated in 15 kg of ethanol, filtered and dried to obtain 101 g of solid content. 15 kg of purified water was added to 101 g of this solid, and after removing particulate impurities using a diatomaceous earth filter and a glass filter, an aqueous solution of 9.5 kg was obtained.

Example 6

After washing the dried Cucumber root (Dish Root) well without any foreign matter, semi-dry it in a ventilated shade, and then put 1kg of fine Cucumber root in 20kg of phosphate buffer solution with pH = 7.4 and extract it at room temperature for 3 days. Thereafter, the filtrate was filtered through a 400 mesh filter cloth, precipitated in 15 kg of ethanol, filtered, and dried to obtain 113 g of solid content. 12 kg of purified water was added to 113 g of this solid, and the resultant was filtered through a glass filter to remove particulate impurities, thereby obtaining 11 kg of an aqueous solution.

Example 7

9.0 kg of purified water was added to 1 kg of the solid content obtained by the method of Example 1, followed by stirring and dissolving with a stirrer to obtain 10 kg of an extract of mucoid muscle extract solution containing 10% by weight of Takyu root extract solid.

Prescription Example  One

The composition of the flexible lotion (skin) in the cosmetic containing the aqueous solution of the myocardium root extract of Example 7 is shown in Table 1 below:

number Raw material Content (% by weight) One Cucumber extract mucus solution 10.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Polyoxyethylene Cured Castor Oil 1.0 5 ethanol 10 6 antiseptic 0.2 7 Spices 0.2 8 Purified water 73.6

<Production method>

Referring to Table 1, in the process of preparing the flexible lotion containing the chylochome extract slime, 8 and 2 were added in order and stirred to dissolve, followed by heating to 60 ° C. for 4 to dissolve it. Dissolve in 7 times and dissolve in 8 times. Then, add 6 times to 5 to dissolve, and then to 8 times. Finally, add 1 to 8 times and stir enough.

Prescription Example  2

The composition of the nutrient lotion (loshon) in the cosmetic containing the aqueous solution of the myocardium root extract of Example 7 is shown in Table 2 below:

number ingredient Content (% by weight) One Cucumber extract mucus solution 10.0 2 Stearic acid 1.0 3 Cetyl alcohol 1.0 4 Glyceryl Monostearate 1.0 5 Polyoxyethylene sorbitan monostearate 1.0 6 Sorbitan sesquioleate 1.0 7 Liquid paraffin 4.0 8 Squalane 3.0 9 Carlylic / Capric Triglycerides 4.0 10 Carboxy Vinyl Polymer 0.1 11 Butylene glycol 5.0 12 Triethanolamine 0.2 13 antiseptic 0.4 14 Spices 0.1 15 Purified water Remaining amount system 100

<Production method>

Referring to Table 2, the process for preparing the nutrient lotion containing the extract of mucosa muscle was heated to 80 ~ 85 ℃ while stirring and mixing 10, 11, 13, and 15 and put into the manufacturing unit and then actuated the emulsifier , 2, 3, 4, 5, 6, 7, 8, and 9 were dissolved after heating to 80 ~ 85 ℃. After emulsification, cool down to 50 ℃ with stirring using a stirrer, add 12 times, cool to 45 ℃, add 14 times, add 1 to 35 ℃, and cool to 25 ℃ before aging.

Prescription Example  3

The composition of the nourishing cream in the cosmetic composition containing the aqueous solution of the myocardium root extract of Example 7 is shown in Table 3 below:

number Raw material Content (% by weight) Prescription Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 One Cucumber extract mucus solution 10 - - - - - 2 water - One - - - - 3 Indomethacin - - One - - - 4 Machi Prefecture Extract - - - One - - 5 Hyaluronic acid sodium salt - - - - One 6 1,3-butylene glycol - - - - - One 7 Beeswax 8 8 8 8 8 8 8 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 1.5 9 Sorbitan sesquioleate 0.5 0.5 0.5 0.5 0.5 0.5 10  Liquid paraffin 20 20 20 20 20 20 11  Sorbitan stearate One One One One One One 12  Lipophilic Monostearate Glycerin 0.5 0.5 0.5 0.5 0.5 0.5 13  Stearic acid 1.5 1.5 1.5 1.5 1.5 1.5 14 Glyceryl Stearate / Fiji 400 Stearate One One One One One One 15 antiseptic 0.2 0.2 0.2 0.2 0.2 0.2 16  Triethanolamine 0.2 0.2 0.2 0.2 0.2 0.2 17  Propylene glycol 6 6 6 6 6 6 18 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount 19 Spices 0.1 0.1 0.1 0.1 0.1 0.1

Referring to Table 3, the manufacturing process of the nutrient cream containing the extract of mucoid muscle muscle was heated to 80 ~ 85 ℃ while mixing and stirring No. 17 and No. 18 put into the manufacturing unit, and then actuated emulsifier 7, 8, 9 , 10, 11, 12, 13, 14, and 15 times to 80 ~ 85 ℃ heated to emulsify. After emulsification, 16 times were administered and neutralized and 19 times cooled to 50 ° C. were added while stirring using a stirrer.

Comparative example  1 to 3

Instead of containing the aqueous solution of the myocardium extract mucosa of Formulation Example 3, water (Control 1), sodium hyaluronate salt (Comparative Example 2), and 1,3-butylene glycol (Comparative Example 3) were used at 10% by weight. Except that the nutrition cream was prepared in the same composition.

Experimental Example  One - Cilia  Cell Proliferation Effect of Extracted Mucus

For Example 1 was measured for cell proliferation and toxic effects using cell culture.

1) Experiment Method

Cell proliferation and toxicity experiments were carried out in the following manner. Incubate the cells (fibroblast, 3T3) in 96-well microplates at 5,000 cells / well and incubate in a thermostat for 30 minutes, and sample is 0.01, 0.05, 0.1, 0.5, 1.0 (%, W / V) for each concentration. )) And incubated for 72 hours. Thiazoline blue was administered and further cultured for 4 hours. All cultures were discarded, the reaction stopper was added to each well of the microplate, and stirred for 5 minutes, and then absorbance was measured at 570 nm. As the control group, 10% Fetal Bovine Serum (FBS) medium was used as the sample injection amount to co-culture the optimal conditions for cell growth. .

2) Experiment result

The cell proliferation effect was calculated by Equation 1 and the results are shown in Table 4 and plotted in the graph of FIG. 3:

density(%) Control 0.001 0.005 0.01 0.05 0.1 0.5 1.0 Proliferation rate (%) 100 103.7 110.5 132.9 145.3 161.7 169.6 175.3

Referring to Table 4, it was confirmed that the extract of Chok-Geun muscle was very excellent in the proliferation effect of the cells. In addition, results confirming that there is no toxicity of the cells.

Experimental Example  2 - Cilia  Anti-inflammatory of extractive mucus

Carrageenin is a polysaccharide extracted from edible seaweed (Irish moss) and is widely used as an edema inducing agent. Serotonin and histamine are released from mast cells within 1 hour after carrageenan administration to induce an allergy reaction. Increase permeability After 2 hours of administration, prostaglandin-based substances are secreted to enhance edema and reach the peak. This is an inflammation-related reaction, confirming the anti-inflammatory effect of Takyu muscle extract mucus (Ref. 참고 物 抽出 物 の 抗 ア レ ル ギ-作用. 西部 幸 修, 島 島 小, Fragrance Journal臨 時 增刊 (16): 109-112, 1999, Studies on the phramcological action of cactus: Identification of its anti-inflammatory effect.Eun-Hee Park et al., Arch Pharm Res 21 (1): 30-34, 19)

1) Experiment Method

Two hours and one hour before the injection of 2% carrageenan saline, 100 mg of the sample was applied to the right foot and rubbed about 50 times with the index finger. The thickness of the foot was measured before applying the sample to calculate the foot edema rate. The experiment was divided into the efficacy test of the sample itself and the test of the application of the sample to the product.The negative control in the sample self-efficacy experiment was water, the positive control was indomethacin, and cosmetics were generally used for anti-inflammatory effects. March extract was applied, the negative control in the efficacy test of the product applied in the control example 1, the positive control was applied to Comparative Example 2, and Comparative Example 1 in Comparative Example 1 in the same manner.

25 μl of 2% carrageenan saline was injected into the sole of the foot, and the thickness of the foot was measured in units of 1 hour for 6 hours. Foot thickness was measured using an electronic digital thickness gauge CHICAGO BRAND, USA.

2) Experiment result

The anti-inflammatory effect was calculated by the equations (2) and (3). Foot swelling (%) was calculated by equation (2). By comparing the average foot swelling rate of the mouse with the foot swelling rate of the control group, the inhibition rate (%) of foot edema was calculated by Equation 3:

Ec: thickness of foot before injection, E: thickness of foot after injection

The results of foot swelling (%) and foot swelling inhibition (%) according to the sample itself and applied products are shown in Table 2 and Table 3 below. 4 and 5 graphically depicted:

Time (hours) Incidence and inhibition of foot edema in the sample itself (%) N. Control Water P. Control Indomethacine Cilia March Incidence Suppression rate Incidence Suppression rate Incidence Suppression rate 1 hr 50.37 ± 7.8 30.22 ± 4.98 40 ± 8.00 33.73 ± 5.77 33.04 ± 9.8 47.83 ± 6.39 5.04 ± 10.85 2 hr 60.89 ± 7.21 39.89 ± 9.14 34.49 ± 13.95 41.97 ± 4.63 31.07 ± 7.2 60.32 ± 5.47 0.94 ± 8.51 3 hr 62.32 ± 4.99 32.33 ± 4.37 48.12 ± 6.61 40.32 ± 5.14 35.3 ± 8.18 55.73 ± 6.22 10.57 ± 9.89 4 hr 55.31 ± 5.48 25.72 ± 2.59 53.5 ± 4.42 31.98 ± 2.4 42.18 ± 4.37 57.46 ± 7.44 3.89 ± 13.56 5 hr 54.21 ± 5.00 18.39 ± 2.83 66.08 ± 3.94 28.14 ± 1.58 48.09 ± 2.72 52.38 ± 6.38 -3.38 ± 10.97 6 hr 56.29 ± 5.68 14.66 ± 2.04 73.96 ± 3.48 22.52 ± 2.45 59.99 ± 4.35 49.83 ± 5.59 11.48 ± 9.93

Time (hours) Incidence and inhibition rate of foot edema of applied product (%) Control Example 1 (N. Control) Control Example 2 (P. Control) Prescription Example 3 Comparative Example 1 Incidence Suppression rate Incidence Suppression rate Incidence Suppression rate 1 hr 38.21 ± 5.07 31.24 ± 2.36 18.24 ± 6.66 35.33 ± 5.25 7.54 ± 10.32 30.75 ± 4.76 19.52 ± 3.97 2 hr 42.94 ± 3.84 34.65 ± 3.12 19.31 ± 7.09 31.67 ± 3.07 26.25 ± 6.76 36.97 ± 5.69 13.90 ± 7.49 3 hr 49.67 ± 4.86 28.23 ± 4.38 43.16 ± 10.21 29.81 ± 5.88 39.98 ± 5.38 39.09 ± 3.56 21.30 ± 6.58 4 hr 51.47 ± 4.9 23.86 ± 5.78 53.64 ± 8.34 25.42 ± 2.32 50.61 ± 8.12 35.68 ± 6.33 30.68 ± 2.08 5 hr 55.33 ± 4.56 19.44 ± 5.09 64.87 ± 9.09 22.86 ± 4.88 58.68 ± 6.9 30.37 ± 2.03 45.11 ± 5.71 6 hr 52.31 ± 4.45 15.89 ± 3.9 69.62 ± 5.78 17.49 ± 3.89 66.56 ± 4.21 28.95 ± 3.22 44.66 ± 9.01

Referring to Table 5 and Table 6, the edema inhibitory effect of the extract was weaker than the positive control group indomethacin, but has a much better edema inhibitory effect than the March extract currently used in the cosmetic industry. Could confirm. In addition, when applied to the product it was confirmed that there is an excellent effect of the same aspect as when only the sample itself was tested.

Experimental Example  3-improve skin itching and Anti-atopic dermatitis  effect

1) Experiment Method

The clinical evaluation of the atopy skin improvement effect on the cosmetics of Formulation Example 3 was measured. Prescription Example 3 The following experiment was performed on 40 children and adults who visited a Korean medical center and a hospital with atopic skin to evaluate the improvement effect of the atopic skin. The test sample was applied to the whole body by right and left, respectively, by double-blinded test, but the use of other moisturizers that could affect the effect of the test sample was prohibited as much as possible. The effects after 1, 2, 4, 8, and 12 weeks of application of the test samples were evaluated by SCORAD (SCORAD: SCORing Atopic Dermatitis) measurement.

2) criteria

① Extent criteria: Area = damage area / 100

② intensity criteria

   -Erythema (1/2/3)

   -Edema (1/2/3)

   -Exudate (1/2/3)

   -Skin peeling (1/2/3)

   Taekwondo degree (1/2/3)

   Drying degree (1/2/3)

Subjective criteria

   -Itching (1-10)

   Insomnia (1-10)

* Scorad calculation = (accuracy criterion / 5) + (strength criterion / 7) + subjective criterion

3) Experiment result

The results of measuring the clinical evaluation of the atopic skin improvement effect on the cosmetic formulations of Formulation Example 3, Comparative Example 1 and Control Example 1 are shown in Table 7:

Classification Week 0 2 weeks 4 Weeks 8 Weeks 12 Weeks Prescription Example 3 70.3 36.3 15.9 10.2 4.1 0.00% 48.36% 77.38% 85.49% 94.17% Comparative Example 1 69.5 57.1 49.5 38.1 31.2 0.00% 17.84% 28.78% 45.18% 55.11% Comparative Example 1 71.4 59.5 53.6 44.8 36.2 0.00% 16.67% 24.93% 37.25% 49.30%

Referring to Table 7, it can be seen that the cosmetic composition of Formulation Example 3, which contains the extract of Cucumis root, has a very good atopic skin improvement effect compared to Comparative Example 1 and Comparative Example 1. In particular, the results after 12 weeks showed a very good atopic skin improvement effect of 94.17%. The improvement rate of atopic skin is shown in FIG. 6. In addition, the clinical results of the before and after use of the cosmetic containing the extract of Chokkeukgi are shown in Figure 7.

Meanwhile, in order to examine the atopic dermatitis alleviating effect felt by the caregiver, after 12 weeks of using the cosmetics of Formulation Example 3, Comparative Example 1, and Control Example 1, the parent or the user of the dermatitis itching, erythema change, It was shown in Table 9 to evaluate the degree of improvement of the dermatitis and the side effects caused by the test sample along with the satisfaction with the dry skin, and the results are shown in the graph in FIG.

(Criteria)

One 2 3 4 5 no effect Some usually Great Very good Very dissatisfied dissatisfaction usually satisfied very good

Classification Itching of the skin Erythema change Dry skin Overall improvement Side Effect Prescription Example 3 4.01 3.96 3.70 4.36 4.04 Comparative Example 1 2.55 3.27 3.65 3.22 3.97 Comparative Example 1 3.00 3.31 3.52 3.36 3.94

Referring to Table 9, it can be confirmed that the cosmetic composition of Formulation Example 3 according to the present invention has an improvement effect in all evaluation items for a user or a protector having atopic skin as a test subject.

Experimental Example  4 - Cilia  Extract mucus moisture Ability  Measure

The moisture retention capacity of the sample was confirmed by measuring the rate at which the mucus extracted from the cecum muscle absorbed or dried at a constant temperature and constant humidity. In order to confirm the water retention, experiments were carried out using water, sodium hyaluronate salt and 1,3 butylene glycol and products containing them as comparative examples. This is an experiment to check the water evaporation rate at 15% and 55% relative humidity, and the more the water evaporates, the lower the ability of the sample to contain water.

Relative humidity of 15% is to check the water retention of the sample under very low humidity conditions, and relative humidity of 55% is the average humidity condition in our daily lives. will be. This is the effect that is linked to moisturizing in cosmetics, so the lower the evaporation of moisture, the better the effect. In addition, each sample was applied to the same formulation to confirm the moisture retention in the product. (Reference: Moisturizing effect of 北山 雅 一, Polypeptide, Fragrance J. January 1995, pp. 99-103, Patent 10-0445430, Moisturizing cosmetics containing extracted polysaccharides)

1) Experiment Method

Prepare four sets of glass chambers (with lids) with a chamber inside dimension of 240 × 130 × 220 mm and a thickness of 30 mm. Fill the two glass chambers with silica gel (Blue medium granular) to a certain height. Two glass chambers were charged with 2.5 g of magnesium chloride to 100 g of purified water to fill a saturated aqueous magnesium chloride solution, and the relative humidity (RH) was measured using an electronic thermo-hygrometer at 25 ° C. RH) is 15% glass chamber containing silica gel blue, 55% glass chamber containing a saturated aqueous solution of magnesium chloride.

Beakers to store samples in the chamber should measure the inside diameter and height, and prepare four 10 ml beakers per chamber with no significant differences.

Each concentration of the sample used for the measurement is as follows.

<Moisturizing power of the sample itself>

① water.

② 1.0% aqueous solution of mucus muscle extract mucus solids.

③ 1.0% aqueous solution of sodium hyaluronate salt.

④ 1.0% aqueous 1,3 butylene glycol solution.

<Moisturizing power of the product which applied a sample>

① Product that 10% by weight of water is applied to basic composition.

② The product to which 10% by weight of 1.0% aqueous solution of chylomuscle extract mucus solids is applied.

③ Hyaluronic acid sodium salt 1.0% 10% by weight aqueous solution product is applied.

④ 10% by weight of 1,3 butylene glycol 1.0% aqueous solution.

The measurement was made in hours (hr), and the measurement time was 5, 10, 20, 30, 40, 50, 62 hours. The time was taken as the end time of the complete evaporation of the sample, the sample amount was tested taking 5g. The method is a modification of the experiments of the previously registered patent 10-0445430.

The weight was measured at a time interval determined by the above method. The evaporation amount was calculated by calculating the evaporation rate by subtracting the weight of the sample by time from the initial sample weight. The samples themselves were measured five times for each condition of 15% and 55% of R.H. The samples were used five times for each condition of 15% and 55% of R.H.

2) Experiment result

The water evaporation rate was calculated by Equation 4, and the results are shown in Tables 6, 7, 8, and 9, and the water evaporation rates at 15% and 55% relative humidity of each sample were plotted in FIGS. 9 and 10. Water evaporation rates at 15% and 55% relative humidity for each application are plotted in FIGS. 11 and 12:

Water evaporation rate (%) for each sample at 15% relative humidity Time (min) Water (control) Cilia Hyaluronic Acid Sodium Salt 1,3-butylene glycol 0 0 0 0 0 5 10.01 ± 1.25 7.83 ± 0.38 9.75 ± 0.44 9.49 ± 0.71 10 25.71 ± 0.33 17.58 ± 1.65 22.93 ± 2.89 23.87 ± 1.77 20 47.79 ± 0.69 39.05 ± 2.74 44.61 ± 2.57 45.72 ± 2.89 40 72.08 ± 1.57 60.48 ± 3.14 67.57 ± 2.96 70.11 ± 2.73 62 98.82 ± 1.84 87.69 ± 3.07 91.39 ± 3.88 94.98 ± 3.35

Water evaporation rate (%) for each sample at 55% relative humidity Time (min) Water (control) Cilia Hyaluronic Acid Sodium Salt 1,3-butylene glycol 0 0 0 0 0 5 5.86 ± 0.98 4.52 ± 1.22 5.73 ± 0.33 5.91 ± 0.39 10 13.76 ± 1.07 10.13 ± 3.06 12.89 ± 1.18 12.37 ± 1.47 20 28.29 ± 1.99 20.57 ± 2.78 27.56 ± 2.49 25.88 ± 2.05 40 50.89 ± 3.23 38.83 ± 2.09 48.75 ± 3.58 48.69 ± 1.28 62 59.83 ± 2.51 47.39 ± 3.75 58.74 ± 1.96 56.93 ± 1.99

Water evaporation rate (%) by product at Relative humidity 15% Time (min) Comparative Example 1 Prescription Example 3 Comparative Example 2 Comparative Example 3 0 0 0 0 0 5 7.21 ± 0.27 5.88 ± 0.73 7.04 ± 0.22 8.05 ± 0.55 10 16.92 ± 2.09 12.74 ± 1.78 15.29 ± 1.49 14.42 ± 2.81 20 30.91 ± 0.98 26.59 ± 2.64 31.67 ± 2.08 29.29 ± 2.32 40 50.34 ± 1.75 44.36 ± 3.02 51.3 ± 2.17 48.07 ± 1.29 62 64.92 ± 2.97 56.78 ± 4.33 64.38 ± 3.69 63.26 ± 3.25

Water evaporation rate (%) by product at 55% Relative humidity Time (min) Comparative Example 1 Prescription Example 3 Comparative Example 2 Comparative Example 3 0 0 0 0 0 5 4.35 ± 0.14 3.42 ± 0.42 4.07 ± 0.41 4.55 ± 0.54 10 12.09 ± 0.47 8.9 ± 1.03 11.73 ± 0.93 10.96 ± 0.66 20 25.98 ± 1.94 20.24 ± 1.02 24.79 ± 1.35 25.02 ± 1.48 40 43.36 ± 2.01 36.83 ± 2.05 43.71 ± 1.81 42.67 ± 2.36 62 54.68 ± 1.55 49.45 ± 1.22 55.24 ± 2.09 54.02 ± 1.39

Referring to Table 10, in the case of hyaluronic acid and 1,3-butylene glycol as a control group, the water evaporated more than 90% after 62 hours at a relative humidity of 15%, while 87% of the extract of Takmus root. It was confirmed that there was considerable water retention by evaporation of. Referring to Table 11, it can be seen that even in the relative humidity of 55%, the extract of Chok-kyun-ku is superior in water retention by 10% than the control and the comparison group.

In addition, referring to Table 12 and Table 13, it was confirmed that the product to which the Tak Kyu-Geun extract, which is the result of water retention in the product to which the Tak Kyu-Geun extract and the comparative material were applied, has better water retention than the comparative group.

Experimental Example  5- Cilia  Extracting mucous skin Moisturizing  Measure

Moisturizing power was measured when the mucus extracted from the muscle muscle was applied to the skin. Experiments using water as a control example, sodium salt of hyaluronic acid and 1,3-butylene glycol as a comparative example, and products containing them (prescription example 3, control example 1, comparative example 2, 3) to confirm skin moisturizing power It was. The instrument used a Corneometer CM820. (Manufacturer: COURAGE + KHAZAKA West Germany)

1) Experiment Method

Samples were prepared using the same sample as Experimental Example 3, and the area of 40mm circle with the left and right arms as the measurement site for 10 subjects in the skin equipment measuring room maintained at a room temperature of 20-25 ° C and a relative humidity of 45-55%. When measuring the sample itself, measure 0.05ml / circle and 0.05g / circle when measuring the product. Pressing the probe (probe) of the corneometer in close contact with the skin surface of the measurement site, the value is displayed, and after the measurement was measured at regular intervals while wiping the probe surface with a soft tissue. Note that the Corneometer CM 820 should be measured with the same force at all times. A certain amount of each sample was taken to confirm the pattern and the time of complete evaporation, and the initial measurement time and the measurement time interval were determined. The chamber was kept at constant temperature and humidity (temperature 20-25 ° C, relative humidity 45-55%) and stayed in the chamber at least 30 minutes before the subject was measured. A circle with a diameter of 40 mm was marked on a certain part of both arms, and the initial skin humidity was measured and recorded using the device. Five measurements were made per circle. Skin moisturizing power of the sample itself was measured after the initial skin humidity was measured by applying a 0.05ml per circle sample to the skin humidity after 2 minutes. A total of five measurements were recorded and recorded at 2 minute intervals. The number of measurements in each period was also recorded by measuring five times. A total of 20 minutes was measured and the results were statistically processed. In the case of the sample itself, it is difficult to see the effect for a long time because the evaporation rate is fast. Therefore, skin humidity change of 20 minutes was observed. In addition, the skin moisturizing power of the product to which each sample was applied was measured after the initial skin humidity was measured and the skin humidity was measured after 5 minutes by applying 0.05g per circle. A total of five measurements were recorded and recorded at 20 minute intervals. The number of measurements in each period was also recorded by measuring five times. A total of 100 minutes were measured and the results were statistically processed.

2) Experiment result

The moisturizing power increase rate was calculated by Equation 5, the results are shown in Table 10 and Table 11, the moisturizing power increase rate for each sample and the moisturizing power increase rate for each applied product is plotted in the graph of FIG.

Moisture increase rate for each sample (by cutometer) Minutes Water (control) Cilia Hyaluronic Acid Sodium Salt 1,3-butylene glycol 0 0.00 0.00 0.00 0.00 2 17.37 ± 0.35 38.92 ± 1.34 24.43 ± 1.04 25.66 ± 1.02 4 8.28 ± 0.93 27.08 ± 0.91 18.16 ± 0.31 17.39 ± 1.37 6 6.38 ± 0.27 19.75 ± 1.85 13.88 ± 0.52 12.06 ± 1.19 8 5.07 ± 1.09 14.79 ± 1.02 9.47 ± 0.66 9.47 ± 0.97 10 4.46 ± 0.47 9.01 ± 0.82 9.86 ± 1.49 5.82 ± 1.05 12 5.76 ± 0.66 8.27 ± 0.24 8.53 ± 1.27 4.93 ± 1.29 14 2.96 ± 1.39 9.00 ± 0.73 5.92 ± 1.00 4.88 ± 0.57 16 1.73 ± 0.58 7.11 ± 1.55 2.79 ± 1.07 5.03 ± 1.04 18 1.85 ± 0.21 7.95 ± 0.84 2.23 ± 0.85 4.21 ± 0.67 20 0.86 ± 0.88 5.88 ± 0.27 2.47 ± 0.98 2.06 ± 1.03

Moisture Growth Rate by Application (by Cutometer) Minutes Comparative Example 1 Prescription Example 3 Comparative Example 2 Comparative Example 3 0 0.00 0.00 0.00 0.00 5 21.35 ± 1.03 27.35 ± 1.22 20.32 ± 1.94 23.27 ± 1.87 25 27.03 ± 1.06 33.65 ± 0.73 30.77 ± 1.01 28.66 ± 0.85 45 23.51 ± 0.94 29.37 ± 1.11 21.79 ± 0.86 21.71 ± 1.05 65 15.87 ± 0.97 25.91 ± 0.86 17.09 ± 1.20 16.85 ± 1.33 85 12.39 ± 0.75 22.48 ± 1.05 16.47 ± 0.99 13.96 ± 0.96 100 11.96 ± 0.37 24.56 ± 0.99 14.22 ± 1.61 14.29 ± 1.49

Referring to Table 14 and Table 15, the product to which the extract was applied to the extract and the extract of the extract was excellent hyaluronic acid, 1,3-butylene glycol compared to the control group water and excellent moisturizing power compared to the rate of increase in moisturizing power and It was confirmed that there was a maintenance effect.

According to the present invention, the mucus extracted and isolated from the muscle muscle and the product containing it are excellent in moisturizing and skin trouble improvement effect. In addition, there is a cell proliferation effect, anti-inflammatory, itching inhibition, and anti-atopic effect can be used in various cosmetic compositions because it enhances the skin barrier function by improving and preventing various inflammatory problems and diseases of the skin.

Claims (6)

delete delete A cosmetic composition for moisturizing skin containing tactile root extract of the mucus component prepared by immersing the endospertic muscle in water at 5 to 40 ° C. for 1 to 15 days to extract the mucus component and then precipitating it in ethanol. delete The method of claim 3, Cosmetic composition, characterized in that the chokeun muscle extract is formulated in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. The method of claim 3, Cosmetic composition characterized in that it has a formulation of softening cream, nutrient cream, nutrition cream, massage cream, essence, or pack.

Images