JP2007084448A - Production-accelerating agent of hyaluronic acid and skin lotion - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a production-accelerating agent of hyaluronic acid having an excellent hyaluronic acid production-accelerating activity, and skin lotion blended with the same. <P>SOLUTION: This production-accelerating agent contains a plant selected from Solena amplexicaulis (Lam.) Gandhi, Laggera alata (D. Don) Sch.-Bip. ex Oliv, Pedilanthus tithymaloides (L.) Poit., Kalimeris indica (L.) Sch.-Bip., Anredera cordifolia (Tenore) Steen and Bougainvillea glabra Choisy, or its extract. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a hyaluronic acid production promoter having an action of promoting hyaluronic acid production ability in humans using a plant extract, and a skin external preparation containing the same.

  In recent years, research on aging has been promoted. As for the cause of skin aging, aging is an important factor when viewed macroscopically, but in addition to that, the effects of drying, oxidation, sunlight (ultraviolet rays) and the like are also directly related to skin aging. As specific phenomena of skin aging, reduction of mucopolysaccharides such as hyaluronic acid, cross-linking reaction of collagen, cell damage due to ultraviolet rays, and the like are known.

  Among them, hyaluronic acid retains moisture in the cell gap, retains cells based on the formation of a jelly-like matrix in the tissue, retains the lubricity and flexibility of the tissue, and resists external forces such as mechanical failure. And has many functions such as prevention of bacterial infection (see Non-Patent Document 1). For example, it is said that the amount of hyaluronic acid in the skin decreases with aging, and along with this, skin aging such as fine lines and roughness appears. For this reason, many cosmetics containing hyaluronic acid and collagen have been proposed as an ameliorating agent for such aging skin. However, these conventional cosmetics only exhibit a moisturizing effect on the skin surface, and cannot essentially improve aging skin. In addition, cosmetics containing various vitamins and herbal medicines as skin cell activators have been proposed, but these have not yet been able to improve and treat aging skin.

  Furthermore, the hyaluronic acid contained in the joint fluid covers the surface of the articular cartilage and is useful for smooth operation of the joint function. Hyaluronic acid concentration in normal human joint fluid is about 2.3 mg / ml, but in the case of rheumatoid arthritis, hyaluronic acid concentration in joint fluid is reduced to about 1.2 mg / ml and at the same time the viscosity of joint fluid is Remarkably reduced (see Non-Patent Document 2). In addition, it is known that hyaluronic acid content decreases in pyogenic arthritis and gouty arthritis as in the case of rheumatoid arthritis (see Non-Patent Document 3). In the above diseases, it is conceivable to increase the amount of hyaluronic acid in synovial fluid in order to improve lubrication function, cover / protect articular cartilage, suppress pain, and improve or normalize pathological joint fluid. For example, it has been reported that the above-mentioned improvement is observed when sodium hyaluronate joint injection therapy is performed on patients with rheumatoid arthritis (see Non-Patent Document 4). Similarly, in traumatic arthritis, osteoarthritis and osteoarthritis, the above-mentioned improvement effect has been reported by joint injection therapy with hyaluronic acid. (Refer nonpatent literature 5).

  However, treatment of the above diseases is long-lasting and requires a doctor's prescription. Therefore, an external preparation for skin and a therapeutic agent containing a hyaluronic acid production promoter that can be easily treated in daily life have been desired.

  In the healing process after burn injury, hyaluronic acid is known to increase significantly in the granulation during the period from the beginning of the granulation tissue growing from below the necrotic tissue until the entire tissue is replaced with granulation tissue. (See Non-Patent Document 6), hyaluronic acid production promoters are also expected as an early treatment for burns.

  Conventionally, drugs that produce hyaluronic acid in human cells include cytokines such as insulin-like growth factor-1, epidermal growth factor (see Non-Patent Document 7) and interleukin-1 (see Non-Patent Document 8), Ball esters (see Non-Patent Document 9) and the like are known, but none of them can be used simply and safely as cosmetics, bathing agents, pharmaceuticals and the like. Various plant-derived hyaluronic acid production promoters have been developed in the hope of safety and mild irritation to the skin (see, for example, Patent Document 1).

Japanese Patent Laid-Open No. 11-209261 "Bio Industry", vol.8, p.346 (1991) "Arthritis Rheumatism", vol.10, p.357 (1967) "Combined composition" (Kanehara Publishing), 481, 1984 "Inflammation" (Japan Inflammation Society), 11, 16, 1991 "Connective tissue and diseases" (Kodansha), 246, 1980 "Connective tissue and diseases" (Kodansha), 153, 1980 "Biochemica Biophysica Acta", 1014, p.305 (1989) “Japan Society of Obstetrics and Gynecology” magazine, 41, 1943, 1989 "Experimental Cell Research", vol.148, p.377 (1983)

  The present invention has been made in view of the above circumstances, and a hyaluronic acid production promoter that can be used safely and easily, has the action of promoting hyaluronic acid production ability in humans, and a skin external preparation containing the same. The purpose is to provide.

  The present invention relates to Solena amplexicaulis (Lam.) Gandhi, Laggera alata (D. Don) Sch.-Bip.ex Oliv, Pedilanthus tithymaloides (L.) Poit., Kalimeris indica (L.) Sch.-Bip., Anredera cordifolia (Tenore) A hyaluronic acid production promoter comprising one or more plants selected from Steen and Bougainvillea glabra Choisy, or an extract thereof.

  The present invention also relates to one or more plants selected from Solena amplexicaulis (Lam.) Gandhi, Laggera alata (D. Don) Sch.-Bip.ex Oliv and Kalimeris indica (L.) Sch.-Bip. An external preparation for skin characterized by blending the extract.

  The hyaluronic acid production promoter of the present invention has an excellent hyaluronic acid production promoting action, preventing human skin aging (skin agglomeration and elasticity retention), prevention and treatment of arthritis, etc., initial treatment of burns, etc. It can be effectively applied to.

  The external preparation for skin (including pharmaceuticals, quasi-drugs, and cosmetics) containing the hyaluronic acid production promoter of the present invention promotes the production of hyaluronic acid, which is one of the extracellular matrix components, and provides skin elasticity and elasticity. It has the effect of maintaining wrinkles and preventing wrinkles and maintaining a moist and youthful skin condition.

The best mode of the present invention will be described below.
The hyaluronic acid production promoter according to the present invention preferably consists essentially of the plant extract, but may contain other components.

  Solena amplexicaulis (Lam.) Gandhi used in the present invention is a plant belonging to the genus Cucurbitaceae and the genus Melothria, a perennial weak climbing herb that is 60 to 150 cm long and has no branching. I have a beard. Its distribution ranges from Guangdong, Guangxi, Yunnan and Fujian in China, and is used for conjunctivitis, mumps, swelling and pain in the throat, and tuberculosis of the lymph nodes.

  Laggera alata (D. Don) Sch.-Bip. Ex Oliv used in the present invention is a plant belonging to the genus Compositae (Laggera) and is a perennial sturdy upright herb. Distribution is eastern, southern and southwest of China, and is used for cold fever, vomiting and diarrhea, urethritis, vaginal discharge, hepatitis, gastritis swelling, poisonous snake bite, ulcer swelling.

  Pedilanthus tithymaloides (L.) Poit. Used in the present invention is a plant belonging to the genus Pedilanthus of the Euphorbiaceae family and is a semi-upright subshrub. Commonly cultivated in Guangzhou, Nanning, China. It has a cleansing and detoxifying effect, and is used for settling, hemorrhoids, hemorrhoids, swelling and traumatic bleeding.

  Kalimeris indica (L.) Sch.-Bip. Used in the present invention is a plant belonging to the genus Compositae (Kalimeris) and is a perennial herb. It is 30-50cm in height and has a fluffy stem. Distribution is in most parts of China and is used for colds, influenza, dysentery, gastroenteritis, urinary tract infections, bleeding, etc.

  Anredera cordifolia (Tenore) Steen used in the present invention is a plant belonging to the genus Basellaceae and Anredera, and is a succulent small climbing plant. The leaves are alternating, fleshy, oval, and glossy green with a length of 4-6 cm and a width of 4-5 cm. Used for rheumatic numbness, trauma, frailty after illness.

  The Bougainvillea glabra Choisy used in the present invention is a plant belonging to the genus Nyctaginaceae (Bougainvillea). It is native to Brazil and has a height of 4-5m. The leaves are round to broad egg-shaped, 10 to 15 cm long and have a glossy green color. Used externally and used for trauma.

  As a result of a literature survey on plants used for the hyaluronic acid production promoter of the present invention, there has been no report that any plant has a hyaluronic acid production promoting action so far.

  The plant extract of the present invention can be obtained by a conventional method. For example, the plant extract can be obtained by immersing or heating and refluxing a plant with an extraction solvent, followed by filtration and concentration. The extraction solvent can be arbitrarily used as long as it is a solvent that is usually used for extraction. For example, water, methanol, ethanol, propylene glycol, 1,3-butylene glycol, glycerin and other alcohols, hydrous alcohols, chloroform, Organic solvents such as dichloroethane, carbon tetrachloride, acetone, ethyl acetate, and hexane can be used alone or in combination. The extract obtained by extraction with the above solvent is adsorbed as it is, or the concentrated extract is adsorbed by an adsorption method, for example, a column from which impurities are removed using an ion exchange resin, or a column of porous polymer (for example, Amberlite XAD-2). Thereafter, a product eluted with methanol or ethanol and concentrated can also be used. Further, a partitioning method, for example, an extract extracted with water / ethyl acetate can be used.

  Solena amplexicaulis (Lam.) Gandhi used in the present invention preferably uses roots, but other sites can also be used.

  Laggera alata (D. Don) Sch.-Bip. Ex Oliv used in the present invention preferably uses whole plants or roots, but other parts can also be used.

  Pedilanthus tithymaloides (L.) Poit. Used in the present invention is preferably whole plant, but other sites can also be used.

  Kalimeris indica (L.) Sch.-Bip. Used in the present invention is preferably whole plant or root, but other sites can also be used.

  As for Anredera cordifolia (Tenore) Steen used in the present invention, it is preferable to use whole plants, but other sites can also be used.

  Bougainvillea glabra Choisy used in the present invention preferably uses a stem, but other parts can also be used.

  Any of the above-mentioned plants or extracts thereof thus obtained has an excellent hyaluronic acid production promoting action. Such a plant or an extract thereof is preferably used by blending with a skin external preparation.

  When the above-mentioned plant or extract thereof is blended and used in a skin external preparation, it is preferably blended in the total amount of the external preparation in a dry weight of 0.0005 to 20% by mass, more preferably 0.001 to 10% by mass. . If it is less than 0.0005% by mass, the hyaluronic acid production promoting effect of the present invention is not sufficiently exerted. On the other hand, if it exceeds 20% by mass, it is difficult to make a preparation. Moreover, even if it mixes exceeding 10 mass%, the improvement of the big effect is not recognized.

  The external preparation for skin using the hyaluronic acid production promoter of the present invention is a component that is usually used for external preparations for skin such as cosmetics and pharmaceuticals, in addition to the above components, as long as it does not impair the effects of the present invention. , For example, moisturizers, antioxidants, oils, UV protection agents, surfactants, thickeners, alcohols, powder components, color materials, aqueous components, water, various skin nutrients, etc., as appropriate be able to.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, preservatives such as methylparaben, ethylparaben, butylparaben, caffeine, tannin, Verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, Whitening agents such as ascorbic acid glucoside, arbutin and kojic acid, sugars such as glucose, fructose, mannose, sucrose and trehalose, vitamin A derivatives such as retinoic acid, retinol, retinol acetic acid and notinol palmitic acid It may be blended Domo.

  In addition, the external preparation for skin using the hyaluronic acid production promoter of the present invention can be widely applied to cosmetics, quasi-drugs and the like, particularly preferably cosmetics applied to the outer skin, and the agent As long as it is applicable to the skin, the mold may be any solution, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, ointment, lotion, Arbitrary dosage forms such as gels and aerosols are applied.

  The use form of the external preparation for skin is also optional, for example, facial cosmetics such as lotion, emulsion, cream, pack, makeup cosmetics such as foundation, lipstick, eye shadow, aromatic cosmetics, hair cosmetics, bath preparations. Etc. can be used.

  In addition, the form which the skin external preparation using the hyaluronic acid production promoter of this invention can take in said dosage form and usage form is not limited.

  In addition, since the hyaluronic acid production promoter of the present invention has an excellent hyaluronic acid production promoting ability, it can be used as a preventive treatment for arthritis, an initial treatment for burns, etc., in addition to being used as a skin external preparation as described above. Is possible.

When the hyaluronic acid production promoter of the present invention is administered to the human body as a drug, it may be taken orally so that the daily dose is usually about 100 μg to 10 g, preferably about 10 mg to 1 g. The dosage form is arbitrarily used as a powder, tablet, capsule or the like.
When the hyaluronic acid production promoter of the present invention is used as a pharmaceutical composition, the dosage form is not particularly limited and can be appropriately selected according to the administration route and the like. For example, tablets, capsules, powders, fine granules, granules, solutions, syrups and the like can be mentioned as preparations suitable for oral administration, and injections, drops, Suppositories, inhalants, transdermal absorbents, transmucosal absorbents, patches and the like can be mentioned. The injection may be used for any of intravenous injection, intramuscular injection, subcutaneous injection, infusion and the like.

EXAMPLES Next, although an Example is given and this invention is demonstrated further in detail, the technical scope of this invention is not limited at all by this. In addition, all compounding quantities are the mass%.
Prior to the examples, the test method for the hyaluronic acid production promoting effect of the plant-derived solvent extract of the present invention will be described.

1. Preparation of sample (plant extract) (Production Example 1)
316.4 mL of methanol was added to 31.64 g of Solena amplexicaulis (Lam.) Gandhi root, and the mixture was immersed for 7 days at room temperature and then filtered. The methanol in the filtrate was distilled off to obtain 1.00 g of extract (in terms of dry matter).

(Production Example 2)
318.00 g of methanol was added to 31.80 g of whole grass and roots of Laggera alata (D. Don) Sch.-Bip. Ex Oliv, and the mixture was immersed for 7 days at room temperature and then filtered. The methanol in the filtrate was distilled off to obtain 2.01 g of extract (in terms of dry matter).

(Production Example 3)
305.9 mL of methanol was added to 30.59 g of whole plant of Pedilanthus tithymaloides (L.) Poit., And after immersion for 7 days at room temperature, it was filtered. The methanol in the filtrate was distilled off to obtain 2.30 g of extract (in terms of dry matter).

(Production Example 4)
308.99 g of methanol was added to 30.89 g of whole plant and roots of Kalimeris indica (L.) Sch.-Bip. And immersed for 7 days at room temperature, followed by filtration. The methanol in the filtrate was distilled off to obtain 1.22 g of extract (in terms of dry matter).

(Production Example 5)
302.88g of methanol was added to 30.28g of whole plant of Anredera cordifolia (Tenore) Steen, and after immersion for 7 days at room temperature, it was filtered. The methanol in the filtrate was distilled off to obtain 2.18 g (in terms of dry matter) of the extract.

(Production Example 6)
412.6 mL of methanol was added to 41.26 g of Bougainvillea glabra Choisy stalk, and the mixture was immersed for 7 days at room temperature and filtered. The methanol in the filtrate was distilled off to obtain 1.18 g of extract (in terms of dry matter).

  The following experiments were performed using the plant extracts obtained in Production Examples 1 to 6.

2. Measurement of Hyaluronic Acid Production Promoting Action Human skin-derived immortalized epidermal cells were seeded per well in a 6-well petri dish and cultured for 4 days in a growth factor-containing KGB medium (Kurabo Co., Ltd.). Thereafter, the medium was replaced with 2 ml of the KGB medium containing the plant extract, and further cultured for 4 days. The plant extract concentration in the medium was 0.5 × 10 ^{−3 to} 5 × 10 ⁻³ mass%.

  After the culture, the medium was collected and hyaluronic acid was measured. The hyaluronic acid was measured using a commercially available hyaluronic acid measurement kit (manufactured by Chugai Pharmaceutical Co., Ltd.). Further, the amount of DNA in the petri dish was measured and used as an index of the number of cells. The amount of DNA was measured by a fluorescence measurement method using “Hoechst 33258” (manufactured by Hoechst).

  The hyaluronic acid production promoting action was evaluated by the hyaluronic acid production promoting rate. Hyaluronic acid production promotion rate (%) is the medium containing this extract when the amount of hyaluronic acid per DNA of human skin-derived immortalized epidermal cells (control) cultured in a medium not containing this extract is 100. It was defined as the amount of hyaluronic acid per DNA of cultured human skin-derived immortalized epidermal cells. The results are shown in Table 1.

  In addition, about the plant extract used in the present Example, cytotoxicity was not recognized by the said experimental density | concentration.

  As is apparent from Table 1, the plant extract according to the present invention has an excellent hyaluronic acid production promoting effect.

  Below, the formulation example of this invention is shown further. In addition, the solvent extract of the plant used by each formulation example was obtained by the conventional method. The amount of these extracts is shown by dry weight.

Example 1 Cream (formulation component) (mass%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 7.0
(3) Hydrogenated lanolin 2.0
(4) Squalane 5.0
(5) 2-Octyldodecyl alcohol 6.0
(6) Polyoxyethylene (25 mol)
Cetyl alcohol ether 3.0
(7) Glycerin monostearate ester 2.0
(8) Propylene glycol 5.0
(9) Methanol extract of Solena amplexicaulis (Lam.) Gandhi 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and dissolved, and heated to 70 ° C. (water phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Subsequently, the oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. with good stirring.

Example 2 Cream (formulation component) (mass%)
(1) Solid paraffin 5.0
(2) Beeswax 10.0
(3) Vaseline 15.0
(4) Glycerin monostearate ester 2.0
(5) Polyoxyethylene (20 mol)
Sorbitan monolaurate 3.0
(6) Soap powder 0.1
(7) Borax 0.2
(8) Acetone extract of Laggera alata (D. Don) Sch.-Bip. Ex Oliv 0.05
(9) Ethanol extract of Laggera alata (D. Don) Sch.-Bip. Ex Oliv 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(6) to (7) were added to (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (8) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Next, the oil phase was gradually added to the aqueous phase while stirring to carry out the reaction. After completion of the reaction, the mixture was uniformly emulsified with a homomixer and then cooled to 30 ° C. with good stirring.

Example 3 Emulsion (formulation component) (mass%)
(1) Stearic acid 2.5
(2) Cetyl alcohol 1.5
(3) Vaseline 5.0
(4) Liquid paraffin 10.0
(5) Polyoxyethylene (10 mol)
Monooleate 3.0
(6) Polyethylene glycol 1500 3.0
(7) Triethanolamine 1.0
(8) Carboxyvinyl polymer 0.05
(9) Pedilanthus tithymaloides (L.) Poit. Ethyl acetate extract 0.05
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.01
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was dissolved in a small amount of (13) (A phase). (6) to (7) were added to the remaining (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase for preliminary emulsification, and the A phase was further added and uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. with good stirring.

Example 4 Emulsion (formulation component) (mass%)
(1) Microcrystalline wax 1.0
(2) Beeswax 2.0
(3) Lanolin 20.0
(4) Liquid paraffin 10.0
(5) Squalane 5.0
(6) Sorbitan sesquioleate ester 4.0
(7) Polyoxyethylene (20 mol)
Sorbitan monooleate 1.0
(8) Propylene glycol 7.0
(9) Acetone extract of Kalimeris indica (L.) Sch.-Bip. 10.0
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and heated to 70 ° C. (aqueous phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The aqueous phase was gradually added while stirring the oil phase, and the mixture was uniformly emulsified with a homomixer.

Example 5 Jelly (blending component) (mass%)
(1) 95% ethyl alcohol 10.0
(2) Dipropylene glycol 15.0
(3) Polyoxyethylene (50 mol) oleyl alcohol ether 2.0
(4) Carboxyvinyl polymer 1.0
(5) Caustic soda 0.15
(6) L-arginine 0.1
(7) 50% 1,3-butylene glycol extract from Anredera cordifolia (Tenore) Steen 7.0
(8) Sodium 2-hydroxy-4-methoxybenzophenone sulfonate 0.05
(9) Ethylenediaminetetraacetate / trisodium / dihydrate 0.05
(10) Methylparaben 0.2
(11) Perfume appropriate amount (12) Purified water Residue (Production method)
(4) was uniformly dissolved in (12) (aqueous phase). On the other hand, (7) and (3) were dissolved in (1) and added to the aqueous phase. Next, (2) and (8) to (11) were added thereto, and then neutralized and thickened with (5) and (6).

Example 6 Cosmetic liquid (formulation component) (mass%)
(Phase A)
(1) 95% ethyl alcohol 10.0
(2) Polyoxyethylene (20 mol) octyldodecanol 1.0
(3) Pantothenyl ethyl ether 0.1
(4) Bougainvillea glabra Choisy methanol extract 1.5
(5) Methylparaben 0.15
(Phase B)
(6) Potassium hydroxide 0.1
(Phase C)
(7) Glycerin 5.0
(8) Dipropylene glycol 10.0
(9) Sodium bisulfite 0.03
(10) Carboxyvinyl polymer 0.2
(11) Purified water residue (production method)
The A phase and the C phase were uniformly dissolved, and the A phase was added to the C phase and solubilized. Then phase B was added and filled.

Example 7 Pack (blending ingredients) (mass%)
(Phase A)
(1) Dipropylene glycol 5.0
(2) Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
(Phase B)
(3) Solena amplexicaulis (Lam.) Gandhi methanol extract 0.01
(4) Olive oil 5.0
(5) Tocopherol acetate 0.2
(6) Ethylparaben 0.2
(7) Fragrance 0.2
(Phase C)
(8) Sodium bisulfite 0.03
(9) Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
(10) Ethanol 7.0
(11) Purified water residue (production method)
A phase, B phase, and C phase were uniformly dissolved, and B phase was added to A phase to solubilize. C phase was then added and filled.

Example 8 Solid foundation (blending component) (mass%)
(1) Talc 43.1
(2) Kaolin 15.0
(3) Sericite 10.0
(4) Zinc flower 7.0
(5) Titanium dioxide 3.8
(6) Yellow iron oxide 2.9
(7) Black iron oxide 0.2
(8) Squalane 8.0
(9) Isostearic acid 4.0
(10) POE sorbitan monooleate 3.0
(11) Isocetyl octanoate 2.0
(12) Ethanol extract of Laggera alata (D. Don) Sch.-Bip. Ex Oliv 1.0
(13) Preservative appropriate amount (14) Fragrance appropriate amount (production method)
(1)-(7) powder components are sufficiently mixed with a blender, (8)-(11) oily components, (12), (13), (14) are added to this and kneaded well. Filled and molded.

Example 9 Emulsification foundation (cream type)
(Compounding ingredients) (mass%)
(Powder part)
(1) Titanium dioxide 10.3
(2) Celiteite 5.4
(3) Kaolin 3.0
(4) Yellow iron oxide 0.8
(5) Bengala 0.3
(6) Black iron oxide 0.2
(Oil phase)
(7) Decamethylcyclopentasiloxane 11.5
(8) Liquid paraffin 4.5
(9) Polyoxyethylene-modified dimethylpolysiloxane 4.0
(Water phase)
(10) Purified water 50.0
(11) 1,3-butylene glycol 4.5
(12) Pedilanthus tithymaloides (L.) Poit.
30% 1,3-butylene glycol extract 1.5
(13) Preservative appropriate amount (14) Fragrance appropriate amount (production method)
After the aqueous phase was heated and stirred, the powder part sufficiently mixed and ground was added and homomixed. Furthermore, after adding the heat-mixed oil phase and carrying out a homomixer process, the fragrance | flavor was added while stirring and it cooled to room temperature.

Example 10 Cream (Compounding ingredient) (mass%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 7.0
(3) Hydrogenated lanolin 2.0
(4) Squalane 5.0
(5) 2-Octyldodecyl alcohol 6.0
(6) Polyoxyethylene (25 mol)
Cetyl alcohol ether 3.0
(7) Glycerin monostearate ester 2.0
(8) Propylene glycol 5.0
(9) Methanol extract of Kalimeris indica (L.) Sch.-Bip. 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and dissolved, and heated to 70 ° C. (water phase). On the other hand, (1) to (7) and (9) to (12) were mixed, dissolved by heating, and kept at 70 ° C. (oil phase). Subsequently, the oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. with good stirring.

Example 11 Cream (Compounding ingredient) (mass%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 7.0
(3) Hydrogenated lanolin 2.0
(4) Squalane 5.0
(5) 2-Octyldodecyl alcohol 6.0
(6) Polyoxyethylene (25 mol)
Cetyl alcohol ether 3.0
(7) Glycerin monostearate ester 2.0
(8) Propylene glycol 5.0
(9) Anredera cordifolia (Tenore) Steen methanol extract 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and dissolved, and heated to 70 ° C. (water phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Subsequently, the oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. with good stirring.

Example 12 Cream (formulation component) (mass%)
(1) Solid paraffin 5.0
(2) Beeswax 10.0
(3) Vaseline 15.0
(4) Glycerin monostearate ester 2.0
(5) Polyoxyethylene (20 mol)
Sorbitan monolaurate 3.0
(6) Soap powder 0.1
(7) Borax 0.2
(8) Bougainvillea glabra Choisy
100% 1,3-butylene glycol extract 0.05
(9) Ethanol extract of Bougainvillea glabra Choisy 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(6) to (7) were added to (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (8) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Next, the oil phase was gradually added to the aqueous phase while stirring to carry out the reaction. After completion of the reaction, the mixture was uniformly emulsified with a homomixer and then cooled to 30 ° C. with good stirring.

Example 13 Emulsion (formulation component) (mass%)
(1) Stearic acid 2.5
(2) Cetyl alcohol 1.5
(3) Vaseline 5.0
(4) Liquid paraffin 10.0
(5) Polyoxyethylene (10 mol) monooleate 3.0
(6) Polyethylene glycol 1500 3.0
(7) Triethanolamine 1.0
(8) Carboxyvinyl polymer 0.05
(9) Ethyl acetate extract of Solena amplexicaulis (Lam.) Gandhi 0.05
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.01
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was dissolved in a small amount of (13) (A phase). (6) to (7) were added to the remaining (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase for preliminary emulsification, and the A phase was further added and uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. with good stirring.

Example 14 Emulsion (formulation component) (mass%)
(1) Microcrystalline wax 1.0
(2) Beeswax 2.0
(3) Lanolin 20.0
(4) Liquid paraffin 10.0
(5) Squalane 5.0
(6) Sorbitan sesquioleate ester 4.0
(7) Polyoxyethylene (20 mol) sorbitan monooleate 1.0
(8) Propylene glycol 7.0
(9) Acetone extract of Laggera alata (D. Don) Sch.-Bip. Ex Oliv 10.0
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and heated to 70 ° C. (aqueous phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The aqueous phase was gradually added while stirring the oil phase, and the mixture was uniformly emulsified with a homomixer.

Example 15 Jelly (blending component) (mass%)
(1) 95% ethyl alcohol 10.0
(2) Dipropylene glycol 15.0
(3) Polyoxyethylene (50 mol)
Oleyl alcohol ether 2.0
(4) Carboxyvinyl polymer 1.0
(5) Caustic soda 0.15
(6) L-arginine 0.1
(7) Pedilanthus tithymaloides (L.) Poit. 50% ethanol extract 7.0
(8) 2-hydroxy-4-methoxy
Sodium benzophenone sulfonate 0.05
(9) Ethylenediaminetetraacetate
Trisodium dihydrate 0.05
(10) Methylparaben 0.2
(11) Perfume appropriate amount (12) Purified water Residue (Production method)
(4) was uniformly dissolved in (12) (aqueous phase). On the other hand, (7) and (3) were dissolved in (1) and added to the aqueous phase. Next, (2) and (8) to (11) were added thereto, and then neutralized and thickened with (5) and (6).

Example 16 Cosmetic liquid (formulation component) (mass%)
(Phase A)
(1) 95% ethyl alcohol 10.0
(2) Polyoxyethylene (20 mol) octyldodecanol 1.0
(3) Pantothenyl ethyl ether 0.1
(4) Methanol extract of Kalimeris indica (L.) Sch.-Bip. 1.5
(5) Methylparaben 0.15
(Phase B)
(6) Potassium hydroxide 0.1
(Phase C)
(7) Glycerin 5.0
(8) Dipropylene glycol 10.0
(9) Sodium bisulfite 0.03
(10) Carboxyvinyl polymer 0.2
(11) Purified water residue (production method)
The A phase and the C phase were uniformly dissolved, and the A phase was added to the C phase and solubilized. Then phase B was added and filled.

Example 17 Pack (blending component) (mass%)
(Phase A)
(1) Dipropylene glycol 5.0
(2) Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
(Phase B)
(3) Anredera cordifolia (Tenore) Steen methanol extract 0.01
(4) Olive oil 5.0
(5) Tocopherol acetate 0.2
(6) Ethylparaben 0.2
(7) Fragrance 0.2
(Phase C)
(8) Sodium bisulfite 0.03
(9) Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
(10) Ethanol 7.0
(11) Purified water residue (production method)
A phase, B phase, and C phase were uniformly dissolved, and B phase was added to A phase to solubilize. C phase was then added and filled.

Example 18 Solid foundation (blending component) (mass%)
(1) Talc 43.1
(2) Kaolin 15.0
(3) Sericite 10.0
(4) Zinc flower 7.0
(5) Titanium dioxide 3.8
(6) Yellow iron oxide 2.9
(7) Black iron oxide 0.2
(8) Squalane 8.0
(9) Isostearic acid 4.0
(10) POE sorbitan monooleate 3.0
(11) Isocetyl octanoate 2.0
(12) Bougainvillea glabra Choisy ethanol extract 1.0
(13) Preservative appropriate amount (14) Fragrance appropriate amount (production method)
(1)-(7) powder components are sufficiently mixed with a blender, (8)-(11) oily components, (12), (13), (14) are added to this and kneaded well. Filled and molded.

Example 19 Emulsification Foundation (Cream Type)
(Compounding ingredients) (mass%)
(Powder part)
(1) Titanium dioxide 10.3
(2) Celiteite 5.4
(3) Kaolin 3.0
(4) Yellow iron oxide 0.8
(5) Bengala 0.3
(6) Black iron oxide 0.2
(Oil phase)
(7) Decamethylcyclopentasiloxane 11.5
(8) Liquid paraffin 4.5
(9) Polyoxyethylene-modified dimethylpolysiloxane 4.0
(Water phase)
(10) Purified water 50.0
(11) 1,3-butylene glycol 4.5
(12) Ethanol extract of Solena amplexicaulis (Lam.) Gandhi 1.5
(13) Preservative appropriate amount (14) Fragrance appropriate amount (production method)
After the aqueous phase was heated and stirred, the powder part sufficiently mixed and ground was added and homomixed. Furthermore, after adding the heat-mixed oil phase and carrying out a homomixer process, the fragrance | flavor was added while stirring and it cooled to room temperature.

Example 20 Cream (Compounding ingredient) (mass%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 7.0
(3) Hydrogenated lanolin 2.0
(4) Squalane 5.0
(5) 2-Octyldodecyl alcohol 6.0
(6) Polyoxyethylene (25 mol)
Cetyl alcohol ether 3.0
(7) Glycerin monostearate ester 2.0
(8) Propylene glycol 5.0
(9) Methanol extract of Laggera alata (D. Don) Sch.-Bip. Ex Oliv 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and dissolved, and heated to 70 ° C. (water phase). On the other hand, (1) to (7) and (9) to (12) were mixed, dissolved by heating, and kept at 70 ° C. (oil phase). Subsequently, the oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. with good stirring.

Example 21 Cream (Compounding ingredient) (mass%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 7.0
(3) Hydrogenated lanolin 2.0
(4) Squalane 5.0
(5) 2-Octyldodecyl alcohol 6.0
(6) Polyoxyethylene (25 mol)
Cetyl alcohol ether 3.0
(7) Glycerin monostearate ester 2.0
(8) Propylene glycol 5.0
(9) Pedilanthus tithymaloides (L.) Poit. Methanol extract 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and dissolved, and heated to 70 ° C. (water phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Subsequently, the oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. with good stirring.

Example 22 Cream (Compounding ingredient) (mass%)
(1) Solid paraffin 5.0
(2) Beeswax 10.0
(3) Vaseline 15.0
(4) Glycerin monostearate ester 2.0
(5) Polyoxyethylene (20 mol) sorbitan monolaurate 3.0
(6) Soap powder 0.1
(7) Borax 0.2
(8) Kalimeris indica (L.) Sch.-Bip. Acetone extract 0.05
(9) Kalimeris indica (L.) Sch.-Bip. Ethanol extract 0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(6) to (7) were added to (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (8) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Next, the oil phase was gradually added to the aqueous phase while stirring to carry out the reaction. After completion of the reaction, the mixture was uniformly emulsified with a homomixer and then cooled to 30 ° C. with good stirring.

Example 23 Latex (formulation component) (mass%)
(1) Stearic acid 2.5
(2) Cetyl alcohol 1.5
(3) Vaseline 5.0
(4) Liquid paraffin 10.0
(5) Polyoxyethylene (10 mol)
Monooleate 3.0
(6) Polyethylene glycol 1500 3.0
(7) Triethanolamine 1.0
(8) Carboxyvinyl polymer 0.05
(9) Anredera cordifolia (Tenore) Steen ethyl acetate extract 0.05
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.01
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was dissolved in a small amount of (13) (A phase). (6) to (7) were added to the remaining (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase for preliminary emulsification, and the A phase was further added and uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. with good stirring.

Example 24 Emulsion (formulation component) (mass%)
(1) Microcrystalline wax 1.0
(2) Beeswax 2.0
(3) Lanolin 20.0
(4) Liquid paraffin 10.0
(5) Squalane 5.0
(6) Sorbitan sesquioleate ester 4.0
(7) Polyoxyethylene (20 mol)
Sorbitan monooleate 1.0
(8) Propylene glycol 7.0
(9) Bougainvillea glabra Choisy acetone extract 10.0
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and heated to 70 ° C. (aqueous phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The aqueous phase was gradually added while stirring the oil phase, and the mixture was uniformly emulsified with a homomixer.

  Each of the external preparations for skin of Examples 1 to 24 is excellent in hyaluronic acid production promoting effect, and by applying it to the skin, the skin's elasticity and elasticity are maintained, and the youth with moisture Can maintain a healthy skin condition.

Examples 25-30 (tablets)
Tablets were prepared with the compositions shown in Table 2 below.

(Manufacturing method)
The above ingredients were mixed uniformly and tableted to give 170 mg of 1 tablet according to a conventional method.

Examples 31-36 (injection)
The plant extract, sodium chloride and benzyl alcohol prepared above were dissolved in distilled water in the amounts shown in Table 3 below. The solution was filtered through a filter (pore size 0.2 μm) to produce an injection.

Claims (2)

  Solena amplexicaulis (Lam.) Gandhi, Laggera alata (D. Don) Sch.-Bip.ex Oliv, Pedianthus tithymaloides (L.) Poit., Kalimeris indica (L.) Sch.-Bip., Anredera cordifolia (Tenore) Steen And a hyaluronic acid production promoter comprising one or more plants selected from Bougainvillea glabra Choisy or an extract thereof. Contains one or more plants selected from Solena amplexicaulis (Lam.) Gandhi, Laggera alata (D. Don) Sch.-Bip.ex Oliv and Kalimeris indica (L.) Sch.-Bip. An external preparation for skin characterized by the above.