KR102242340B1 - Composition containing essential oil of Zea mays Flower - Google Patents

Abstract

The present invention relates to a method for extracting essential oil from corn flowers and a composition for external application for skin and a cosmetic composition comprising the essential oil prepared therefrom, wherein the essential oil extracted from corn flowers of the present invention does not exhibit cytotoxicity to epidermal keratinocytes. , It has improved its proliferation and has the effect of promoting the migration of epidermal keratinocytes and the formation of collagen, so it can be used as an external preparation for skin composition and cosmetic composition for proliferation of epidermal keratinocytes, improvement of skin regeneration wrinkles and wound healing. .

Description

Composition containing essential oil derived from corn flowers {Composition containing essential oil of Zea mays Flower}

The present invention relates to a composition comprising essential oil derived from corn flowers, and specifically, to a method for extracting essential oil from corn flowers, and to a composition for external application for skin and a cosmetic composition comprising the essential oil prepared therefrom.

Skin is the tissue that covers the outermost part of the human body. It protects the human body and acts as a barrier to protect the body from external chemical and physical shocks. The skin is composed of three layers: epidermis, dermis, and subcutis, and 90% of the epidermis located in the outermost layer is composed of keratinocytes. When the skin is aged by UV rays or stress or damaged by wounds, its function is weakened, and the skin regeneration process begins. The skin regeneration process begins with an acute inflammatory reaction, proliferation, migration, and proliferation of epidermal cells, and then the formation of new blood vessels and the contraction of connective tissue through the synthesis of collagen. At this time, the migration and proliferation of keratinocytes, which occupy more than 90% of the epidermis, not only provides wound healing and skin regeneration, but also plays an important role in enhancing the elasticity of the skin and forming the basement membrane by inducing collagen synthesis.

On the other hand, plant-derived natural essential oils are volatile chemicals that exhibit pharmacological effects on the human body among secondary metabolites of plants, and are mainly composed of various organic compounds such as terpenes, alcohols, aldehydes, esters, and ketones. The aromatic components of these natural essential oils not only relieve stress, relieve fatigue, and relax muscles through the skin, circulatory system, and olfactory system, but also exhibit antibacterial, immunity, and anti-allergic therapeutic effects. Not only has it been used for treatment and prevention, but it is also used as an ingredient in food flavors and cosmetics.

In addition, maize ( Zea mays ) is a herbaceous plant born in the first year of the Graamineae family, and the corn fruits are rich in nutrients such as dietary fiber, protein, fat, vitamins, lecithin, and carotenoids. to be. Corn fruits are not only used for food, but as research technologies for producing biofuels have recently been developed, research to use them as alternative energy for gasoline is being actively conducted. In addition, among the by-products of corn cultivation, corn beard is used to inhibit radiation-induced oxidative stress, inhibit the production of final glycated products related to diabetes and aging complications, inhibit diabetic nephropathy, antioxidant, diuretic effect, anti-obesity activity studies, etc. Various studies have been conducted on corn beard.

However, research using corn flowers has not been conducted in the field of research on human physiological activity, including skin.

Therefore, the inventors of the present invention confirmed that while studying the in vitro skin regeneration effect by extracting essential oil from corn flowers, the essential oil derived from corn flowers induces the proliferation and migration of skin epidermal keratinocytes to exhibit skin regeneration effects. Completed.

It is an object of the present invention to provide a composition for external application for skin comprising essential oils extracted from corn flowers.

In addition, it is an object of the present invention to provide a cosmetic composition for skin regeneration and wrinkle improvement comprising essential oil extracted from corn flowers as an active ingredient.

In addition, it is an object of the present invention to provide a method for preparing corn flower essential oil.

In order to solve the above problems, the present invention provides a composition for external application for skin comprising essential oil extracted from corn flowers.

In addition, the present invention provides a cosmetic composition for skin regeneration and wrinkle improvement comprising the essential oil extracted from corn flowers as an active ingredient.

In addition, the present invention provides a method for producing corn flower essential oil.

The essential oil extracted from the corn flower of the present invention improves the proliferation of epidermal keratinocytes without exhibiting cytotoxicity to the epidermal keratinocytes, and has the effect of promoting the migration of epidermal keratinocytes and the formation of collagen, so that the epidermal keratinocytes proliferate. , It can be used as a skin external composition and cosmetic composition for skin regeneration wrinkle improvement and wound healing.

1 is a flowchart schematically illustrating a process of extracting essential oil (absolute) from corn flowers of the present invention.
2 is a diagram showing the results of component analysis of essential oil (absolute) extracted from corn flowers.
Figure 3 is a diagram confirming the cytotoxicity of the essential oil (absolute) extracted from corn flowers on the epidermal keratinocytes.
4 is a diagram illustrating the effect of promoting epidermal keratinocyte proliferation of essential oil (absolute) extracted from corn flowers.
5 is a diagram illustrating the effect of promoting epidermal keratinocyte migration of essential oil (absolute) extracted from corn flowers.
6 is a diagram showing an image showing the effect of corn flower-derived essential oil (absolute) on the out growth of collagen sprouting in skin epidermal keratinocytes and a graph showing the quantification thereof.

Hereinafter, the present invention will be described in detail as an embodiment of the present invention with reference to the accompanying drawings. However, the following embodiments are presented as examples of the present invention, and if it is determined that a detailed description of a technique or configuration well known to those skilled in the art may unnecessarily obscure the subject matter of the present invention, the detailed description may be omitted. However, the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of equality interpreted from the description of the claims to be described later and therefrom.

In addition, terms used in the present specification are terms used to properly express preferred embodiments of the present invention, which may vary depending on the intention of users or operators, or customs in the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the contents throughout the present specification. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

Throughout this specification,'%' used to indicate the concentration of a specific substance is (w/w)% for solids/solids, (w/v)% for solids/liquids, and Liquid/liquid is (v/v) %.

In one aspect, it relates to a composition for external application for skin comprising essential oils extracted from corn flowers (Zea mays flower).

In one embodiment, the essential oil may be prepared by extracting corn flowers by an organic solvent extraction method to obtain concrete, and dissolving and filtering it in an organic solvent to remove insoluble wax to obtain absolute.

In one embodiment, the essential oil extracted from the corn flower of the present invention is immersed in the corn flower in an organic solvent; Evaporating and concentrating the immersion liquid to obtain concrete; Dissolving concrete in an organic solvent and filtering to remove insoluble wax; It can be prepared by separating and purifying the absolute through evaporation.

The organic solvent extraction method and the method of separating the absolute from the extract may include all methods that can be modified by one of ordinary skill in the art.

In one embodiment, the essential oil extracted from corn flowers is Heneicosane, Alterungsschutzmittel BKF, pentacosane, Trichloroacetic acid, hexadecyl ester, tetra Tetratriacontane, 17-Pentatriacontene, 1-Docosene, 1-Nonadecene, Cyclooctacosane, Stigmasterol ), beta-sitosterol, ergosta-7,24(28)-dien-3-ol,4-methyl-,(3-β,4-α,5-α)-(Ergosta-7, 24(28)-dien-3-ol,4-methyl-),(3-β,4-α,5-α)-), Viminalol may contain one or more components selected from the group consisting of have.

In one embodiment, the corn flower may be a flower bud just before flowering, and may be a flower that has not been dried.

In one embodiment, the composition for external application for skin of the present invention may be for skin epidermal keratinocyte proliferation, regeneration, and collagen formation, which is elasticity, regeneration, and skin whose physiological activity is reduced due to aging and wrinkles, wounds, and skin lesions. / Or it can be useful for wound healing, etc.

In one embodiment, the composition for external application for skin of the present invention may contain essential oil extracted from corn flowers at a concentration of 1 to 200 μg/mL, and it is best to include the essential oil at a concentration of 50 to 200 μg/mL for skin regeneration and wounding. It is more preferable because it has a healing effect.

The term "essential oil" used in the present invention is a term collectively referring to aromatic oils (volatile substances) extracted and separated from plants (plant leaves, stems, fruits, flowers, roots, etc.), extracts, extraction methods, uses and purposes It is classified in various ways according to. Essential oils derived from natural products are classified into essential oils, absolutes, and concretes according to the extraction method.

The term "extract" as used in the present invention means an active ingredient separated from a natural product, that is, a substance exhibiting a desired activity. The extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes a dry powder of the extract or any form formulated using the same. In addition, the extract includes a fraction obtained by fractionating the extract that has undergone the extraction process. The extraction method of the extract is not particularly limited, and may be extracted by, for example, stirring extraction, shaking extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction. As the extraction solvent, a _{polar solvent such as water or a C 1} -C ₄ lower alcohol, a non-polar solvent such as hexane, chloroform, dichloromethane or ethyl acetate, or a mixture of two or more thereof may be used.

In one aspect, the present invention relates to a wound healing composition comprising essential oil extracted from corn flowers.

In one aspect, the present invention relates to a cosmetic composition for skin regeneration and wrinkle improvement comprising essential oil extracted from corn flowers.

The cosmetic composition of the present invention can be prepared including a cosmetically effective amount of the essential oil extracted from the corn flower of the present invention and a cosmetically acceptable carrier.

As used herein, the term "cosmetically effective amount" refers to an amount sufficient to achieve the effect of skin regeneration through the proliferation of epidermal keratinocytes of the composition of the present invention described above.

The cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, such as solutions, gels, solids, dough anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, for example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing It can be formulated into cosmetics such as cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, as a carrier component, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be used.

The cosmetic composition of the present invention is skin, lotion, cream, essence, pack, foundation, color cosmetics, sun cream, two-way cake, face powder, compact, makeup base, skin cover, eye shadow, lipstick, lip gloss, lip fix, eyebrow pencil , It can be applied to cosmetics such as lotion and detergents such as shampoo and soap.

In addition to the essential oil extracted from the corn flower, the cosmetic composition according to an embodiment of the present invention may further include functional additives and ingredients included in a general cosmetic composition. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

In addition to the above functional additives, the cosmetic composition of the present invention may further contain components contained in a general cosmetic composition, if necessary. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation Examples include accelerators, cooling agents, restrictors, and purified water.

In one aspect, the present invention includes immersing corn flowers in an organic solvent, evaporating and concentrating the immersion liquid to obtain concrete, dissolving and filtering the concrete in an organic solvent to remove insoluble wax, and separating and purifying the absolute. It relates to a method of manufacturing the essential oil of corn flowers.

The present invention will be described in more detail through the following examples. However, the following examples are only for embodiing the contents of the present invention, and the present invention is not limited thereto.

Example 1. Preparation of corn flower essential oil

Hoseo University (Asan, Korea) The whole flower buds just before flowering were collected and washed in July in the vicinity of the practice site, and then extracted by immersing in hexane for 60 minutes without drying, and then using a rotary evaporator. It was concentrated to obtain solid concrete. Thereafter, alcohol was added to the solid concrete to react for 12 hours, and after removing the insoluble wax through a vacuum cooling filter, the corn flower absolute was obtained by separating/purifying again with a rotary evaporator (FIG. 1). . The prepared corn flower absolute was stored at a low temperature of 4°C.

Example 2. Analysis of components of essential oil of corn flowers

Using a gas chromatography/mass spectrometer (6890N GC/5975i MS, Agilent, USA), the components of the corn flower absolute were determined by the retention indices (RI) of the volatile component, the retention time of the standard, and the mass spectrum of the volatile substance. The mass spectrum of the Wiley 7Nist05 library was compared and analyzed. Specifically, DB5-MS (30m×50μm, 0.25μm) was used for the GC/MS column, and the carrier gas was analyzed by flowing at 1 mL/min using helium. The temperature of the oven was maintained at 40℃-2℃/min-230℃-5℃/min-300℃ (5 minutes), and the temperature of the injection was analyzed at 280℃. In addition, the temperature of the interface was analyzed at 300℃, the temperature of the ion source was 230℃, and the temperature of the analyzer was 150℃, and the mass range was analyzed at 40~800m/z. RI was determined under the same conditions as the sample using C6 ~ C24 (n-alkane).

As a result, it was confirmed that the corn flower absolute prepared in Example 1 was composed of a total of 13 components, 1-Nonadecene, Cyclooctacosane, Ergosta-7,24(28)-dien-3-ol,4- methyl-,(3-β,4-α,5-α)-, 1-Docosene, β-Sitosterol, Pentacosane, Viminalol, Heneicosane, Stigmasterol, Tetratriacontane, Trichloroacetic acid, hexadecyl ester, 17-Pentatriacontene, Alterungsschutzmittel BKF in that order It showed a high content (1-Nonadecene 30.37% by weight, Cyclooctacosane 16.14% by weight, Ergosta-7,24(28)-dien-3-ol,4-methyl-,(3-β,4-α,5-α) -11.07 wt%, 1-Docosene 10.27 wt%, β-Sitosterol 8.66 wt%, Pentacosane 5.05 wt%, Viminalol 4.10 wt%, Heneicosane 3.80 wt%, Stigmasterol 2.87 wt%, Tetratriacontane 2.38 wt%, Trichloroacetic acid, hexadecyl ester 2.35 Wt%, 17-Pentatriacontene 1.79 wt%, Alterungsschutzmittel BKF 1.16 wt%) (Figure 2)

Example 3. Confirmation of Cytotoxicity of Corn Flower Essential Oil

It was confirmed whether the corn flower essential oil (absolut) obtained in Example 1 exhibits toxicity to the skin epidermal keratinocytes. Specifically, human skin-derived epidermal keratinocytes (HaCat) were cultured in DMEM medium containing 10% fetal bovine serum, and dispensed into a 96-well plate at 5×10 ³ cells/100 μL/well. Incubated in a 5% CO ₂ incubator at 37°C. Then, 100 μL of corn flower-derived essential oil was treated in each well at a concentration of 0 to 250 μg/mL, and incubated for 24 hours. Ez-cytox was treated with 10 μL per well and reacted for 30 minutes in an incubator, and absorbance was measured at 450 nm using an ELISA plate reader (Synerge 2, BioTek, USA). The measurement results were converted into percentages to calculate the survival rate of the epidermal keratinocytes of the corn flower-derived essential oil.

As a result, no significant cytotoxicity was observed in the corn flower-derived essential oil at a concentration of 1 to 250 μg/mL (FIG. 3).

Example 4. Effect of corn flower essential oil on improving epidermal keratinocyte proliferation

The effect of the corn flower essential oil (absolute) obtained in Example 1 on the proliferation of epidermal keratinocytes was confirmed. Specifically, human skin-derived epidermal keratinocytes (HaCat) were cultured using DMEM medium containing 10% fetal bovine serum, and epidermal keratinocytes were 3×10 ^{3 in a 96-well microplate.} Cells/100 μL/well were dispensed and cultured in _{a 5% CO 2 incubator at 37°C.} Then, 100 μL of corn flower absolute was treated in each well at a concentration of 0 to 200 μg/mL, and incubated for 48 hours. Here, the negative control group was the untreated group without corn flower absolute, and the positive control group used epithermal growth factor (EGF 50 ng/mL), which is conventionally used as a skin regeneration and wound healing agent. Thereafter, 10 μL of Ez-cytox was treated in each well, and then reacted in an incubator for 30 minutes. After measuring the absorbance at 450 nm using an ELISA plate reader (Synerge2, BioTek, USA), the measurement result was converted to a percentage to confirm the result of epidermal keratinocyte proliferation of corn flower Absolut.

As a result, the corn flower-derived essential oil showed a proliferation induction rate of 223.44±7.31% at a concentration of 100 μg/mL, and a proliferation induction rate of 245.56±10.11% at a concentration of 150 μg/mL. It was found to induce proliferation of keratinocytes in a concentration-dependent manner, and in particular, the concentration of 150 μg/mL was found to be higher than 163.69±3.55%, which is the rate of induction of proliferation of epidermal keratinocytes by the epidermal growth factor (Fig. 4). .

Example 5. Effect of corn flower essential oil on improving epidermal keratinocyte migration

The effect of the corn flower essential oil obtained in Example 1 on the migration of epidermal keratinocytes was confirmed. Specifically, in the lower chamber of the 48 well chemotactic boyden chamber, the corn flower absolute was treated with a concentration of 1 to 200 μg/mL (negative control: untreated group and positive control: epidermal growth factor treatment group) and collagen coating was applied. After loading the 8 μm pore polycarbonate membrane, human skin-derived epidermal keratinocytes (HaCat) were dispensed into the upper chamber at 5×10 ⁴ cells/50 μL per well. After incubating for 210 minutes in a 5% CO ₂ incubator at 37°C, the membrane is stained and fixed with Diff-Quick solution, and the number of cells that have moved through the membrane is counted and converted into a percentage. The migration rate of epidermal keratinocytes was calculated.

As a result, it was found that corn flower absolute induces the migration of skin epidermal keratinocytes in a concentration-dependent manner, and in particular, showed a maximum migration rate of 177.32±10.36% at a concentration of 150 μg/mL (FIG. 5).

Example 6. Out growth promoting effect of collagen sprouting of corn flower essential oil

The effect of the corn flower essential oil obtained in Example 1 on the out growth of collagen sprouting was confirmed. Specifically, after mixing Type I collagen and epidermal keratinocytes to a ^{concentration of 2.5×10 7} cells/mL, neutralize by treating with NaOH (1 N) solution, and dispense 5 μL each into a 24-well plate for 5 to 10 minutes. It dried at room temperature to form artificial skin tissue. The formed artificial skin tissue was treated with corn flowers or epithermal growth factor (EGF 50 ng/mL) (positive control) and cultured in a _{37° C., 5% CO 2 incubator for 72 to 90 hours.} Thereafter, the culture medium was removed, cells and tissues were fixed and stained using Diff-Quik solution, and the stained cells and tissues were photographed.

As a result, corn flower Absolut induced out growth of keratinocyte collagen sprouting at 174.73±14.30% at 50 μg/mL concentration and 211.00±11.56% at 150 μg/mL concentration, thereby inducing out growth of keratinocyte collagen sprouting. It was found to be effectively induced and was found to be concentration dependent (Fig. 6).

So far, the present invention has been looked at around its preferred embodiments.

Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the above description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (10)

Contains essential oil extracted from Zea mays flower,
The essential oil is characterized in that it is an absolute solution to remove the insoluble wax after obtaining concrete from the hexane (n-hexane) extract of corn flowers, dissolving it in alcohol, filtering it
The essential oil is characterized in that it comprises a concentration of 50 to 200 μg / mL, wound treatment composition for external application for skin. delete delete The method of claim 1, wherein the essential oil extracted from corn flowers is Heneicosane, Alterungsschutzmittel BKF, Pentacosane, Trichloroacetic acid, hexadecyl ester, and tetratriacontane. ), 17-Pentatriacontene, 1-Docosene, 1-Nonadecene, Cyclooctacosane, Stigmasterol, Beta Sitosterol ( β-Sitosterol), Ergosta-7,24(28)-dien-3-ol,4-methyl-,(3-β,4-α,5-α)-(Ergosta-7,24(28)- Skin for wound treatment, including any one or more components selected from the group consisting of dien-3-ol,4-methyl-),(3-β,4-α,5-α)-), and viminalol Composition for external application. The composition of claim 1, wherein the composition improves the proliferation and migration of epidermal keratinocytes, and induces out growth of collagen sprouting. delete Contains essential oil extracted from Zea mays flower,
The essential oil is characterized in that it is an absolute solution to remove the insoluble wax after obtaining concrete from the hexane (n-hexane) extract of corn flowers, dissolving it in alcohol, filtering it
The essential oil is characterized in that containing 50 to 200 μg / mL concentration, skin regeneration and wrinkle improvement cosmetic composition. The method of claim 7, wherein the essential oil extracted from corn flowers is Heneicosane, Alterungsschutzmittel BKF, Pentacosane, Trichloroacetic acid, hexadecyl ester, Tetratriacontane, 17-Pentatriacontene, 1-Docosene, 1-Nonadecene, Cyclooctacosane, Stigmasterol Stigmasterol), β-Sitosterol, Ergosta-7,24(28)-dien-3-ol,4-methyl-,(3-β,4-α,5-α)-(Ergosta-7 ,24(28)-dien-3-ol,4-methyl-),(3-β,4-α,5-α)-), Viminalol containing one or more components selected from the group consisting of , Cosmetic composition for skin regeneration and wrinkle improvement. delete Immersing corn flowers in hexane (n-hexane) for 50 to 70 minutes;
Evaporating and concentrating the immersion liquid to obtain solid concrete;
Adding the solid concrete to alcohol and reacting for 10 to 12 hours; And
A method for producing a corn flower essential oil comprising a step of removing the insoluble wax to obtain a corn flower absolute (absolute).

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