KR102573041B1 - Composition for skin moisturizing comprising Capsosiphon fulvescens extract as effective component - Google Patents

Abstract

The present invention relates to a composition for moisturizing skin containing an extract of Maesaengi as an active ingredient. Since the extract of Maesaengi of the present invention has excellent skin moisturizing and skin barrier strengthening effects, cosmetics and health functions for restoring the stratum corneum of the skin damaged by external stimuli. It can be usefully used as a food development material.

Description

Composition for skin moisturizing comprising Capsosiphon fulvescens extract as effective component}

The present invention relates to a skin moisturizing composition comprising an extract of Maesaengi as an active ingredient.

The most important function of the epidermis, located in the outermost layer of the skin, is to defend against various external stimuli (physical and chemical stimulants such as chemicals, air pollutants, dry environment, and ultraviolet rays) and to release excessive moisture in the body through the skin. This protective function can maintain its function when the stratum corneum composed of keratinocytes is normally formed. The outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes and is composed of keratinocytes with complete differentiation and a lipid layer surrounding them. Keratinocytes are cells that ascend to the surface of the skin through morphological and functional changes step by step as basal cells in the lowermost layer of the epidermis continuously proliferate. After a certain period of time, old keratinocytes are eliminated from the skin and new Keratinocytes take over their functions, and this repetitive series of changes is called 'differentiation of epidermal cells' or 'keratinization'. In addition, during the keratinization process, keratinocytes form the stratum corneum through the production of natural moisturizing factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acid) to maintain the firmness and flexibility of the stratum corneum, thereby maintaining the skin barrier (skin barrier). function as a barrier).

This stratum corneum can easily lose its function due to lifestyle factors such as excessive washing or bathing, environmental factors such as dry air pollutants, and endogenous diseases such as atopic skin or senile skin. Therefore, in order to maintain proper skin moisture, various studies have been conducted to supply moisture from the outside or to prevent moisture loss from the body, and various moisturizers having the ability to actually retain moisture have been developed. As a skin moisturizer, it is common to use substances capable of increasing water retention in the stratum corneum, such as lipid components such as ceramide, essential fatty acids, and lipid complexes.

Recently, the risk factors for the skin are gradually increasing, and the function of keratinocytes is reduced due to changes in dietary habits, and the amount of moisturizing factors and lipids in the stratum corneum is reduced. This is a growing trend. Disruption of this skin barrier function causes various skin diseases such as xeroderma, atopic dermatitis, contact dermatitis, and psoriasis. Although these diseases can be expected to relieve symptoms through conventional moisturizers having a water retention function, fundamental healing is difficult, and recently, attention has been focused on restoring the most important skin barrier function in the skin rather than simple moisture supplementation.

Maesaengi ( Capsosiphon fulvescens ) is a green algae plant of the order of sea buckthorn, and its body is tubular or flat and dark green. It is a single body with a length of 15 cm and a thickness of 2 to 5 mm . In addition, maesaengi contains a lot of iron, potassium, protein, etc., and has a unique aroma and taste, so it is widely used for food.

On the other hand, Korean Patent Registration No. 0542446 discloses a "cosmetic composition containing Maesaengi extract as a main active ingredient" having whitening, antioxidant, collagen synthesis, and skin cell death inhibitory effects, but containing the Maesaengi extract of the present invention as an active ingredient. There is no description about the composition for moisturizing the skin.

The present invention has been derived from the above needs, and the present inventors prepared an extract using ethanol for falconer, and as a result of treating keratinocytes, the survival rate of cells reduced by ultraviolet irradiation and the skin The present invention was completed by confirming that the expression levels of filaggrin and involucrin proteins related to moisturizing and skin barrier functions were increased.

In order to solve the above problems, the present invention provides a cosmetic composition for moisturizing the skin containing an extract of Capsosiphon fulvescens as an active ingredient.

In addition, the present invention provides a topical skin preparation for preventing or treating skin damage caused by ultraviolet rays, which includes an extract of Maesaengi as an active ingredient.

In addition, the present invention provides a health functional food composition for moisturizing the skin containing an extract of Maesaengi as an active ingredient.

Maesaengi extract of the present invention is a naturally-derived substance, has no cytotoxicity, has low possibility of side effects, and increases the expression of proteins related to cell protection effect against ultraviolet rays and skin moisturizing and skin barrier function reduced by ultraviolet irradiation. is excellent, so it can be usefully used as a material for cosmetics and health functional foods for skin moisturizing and skin barrier reinforcement.

Figure 1 is a result of treating skin keratinocytes (HaCaT) with an ethanol extract of Maesaengi by concentration (25, 50, or 100 μg / ml) and measuring cell viability. is a graph measuring, and B is a graph measuring cell viability after pre-treatment of HaCaT cells with an ethanol extract of Maesaengi and irradiation with ultraviolet (UVB).
Figure 2 is a Western blot result confirming the expression levels of filaggrin (A) and involucrin (B) proteins after pretreatment of HaCaT cells with an ethanol extract of Maesaengi and irradiation with ultraviolet (UVB).

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for moisturizing the skin containing an extract of Capsosiphon fulvescens as an active ingredient.

In the cosmetic composition for moisturizing skin of the present invention, the maesaengi extract may be extracted using water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably a lower alcohol having 1 to 4 carbon atoms, More preferably, it may be extracted using ethanol, but is not limited thereto.

In the cosmetic composition for moisturizing the skin according to one embodiment of the present invention, the extract of Maesaengi is added with 800 to 1,000㎖ of 40 to 60% (v/v) ethanol to 100 to 150g of dried Maesaengi and 38 to 42 It may be prepared by extracting at ° C for 5 to 7 hours, preferably prepared by adding 900 ml of 50% (v / v) ethanol to 150 g of drained raw material and extracting at 40 ° C for 6 hours. It may, but is not limited thereto.

The maesaengi extract may be an extract obtained by extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product or purified product, but is not limited thereto.

In the cosmetic composition for moisturizing skin of the present invention, the maesaengi extract can increase the survival rate of cells reduced by ultraviolet (UVB) irradiation and the expression of filaggrin and involucrin, It is excellent in moisturizing enhancement effect and skin barrier strengthening effect of damaged skin by irradiation.

The term "skin barrier" refers to the upper layer of the epidermis, which is the outermost layer of the skin, and refers to the stratum corneum composed of keratinocytes. It is the most important first line of defense against toxic substances, microorganisms, mechanical stimulation, and ultraviolet rays, and functions to provide an environment in which the skin can perform normal functions by suppressing electrolyte or water loss through the skin. In addition, "strengthening the skin barrier" refers to strengthening the barrier function of the stratum corneum, which is located at the outermost layer of the skin, by increasing the expression of involucrin or filaggrin, thereby increasing the skin moisturizing effect, thereby improving the skin barrier. It means to strengthen the function.

The cosmetic composition of the present invention is any one formulation selected from solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, foundations, wax foundations and sprays. Preferably, skin, skin softener, skin toner, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, eye cream, moisture cream, hand cream, essence, nutrient essence, pack, cleansing It may have any one formulation selected from foam, cleansing water, cleansing cream, body lotion, body cleanser, soap and powder, but is not limited thereto. The cosmetic composition composed of each of these dosage forms may contain various carriers and additives necessary and appropriate for formulation of the dosage form, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, and sarcosinate , fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may further contain excipients including fungicides, hydrotropes, humectants, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.

The present invention also provides an external skin preparation for preventing or treating skin damage caused by ultraviolet rays (ultraviolet) comprising an extract of Maesaengii as an active ingredient.

The external skin preparation of the present invention may be applied to the human body with the extract of the present invention in various ways or forms, but preferably, it may be prepared as a formulation of an external skin preparation that can be applied topically to the skin. For example, powders, ointments, gels, creams, liniments, lotions, liquids, or aerosols may be mentioned, but are not limited thereto.

When formulated as the external skin preparation, it can be prepared by appropriately blending additives and the like in addition to the extract of the present invention. The above additives are commonly used in the art, and may be formulated within a range not impairing the objects and effects of the present invention.

The present invention also provides a health functional food composition for moisturizing the skin containing the extract of Maesaengi as an active ingredient.

In the health functional food composition of the present invention, the maesaengi extract is as described above.

The health functional food for moisturizing the skin of the present invention may provide various types of food additives or functional foods. The health functional food for moisturizing the skin may be processed into, for example, liquid tea, beverages, fermented milk, cheese, jelly, yogurt, juice, probiotic preparations and health supplements, and may be in the form of various food additives depending on other purposes. can be used as In addition, the dosage form of health functional food may be in various forms such as solutions, emulsions, viscous mixtures, tablets, powders, and pills.

Preferably, the health functional food for moisturizing the skin has the advantage of having a more excellent effect by ingesting it in the form of inner beauty food. The inner beauty food is a food referred to as 'eating cosmetics or beauty food', and refers to food that changes the skin constitution to be healthy by absorbing various ingredients good for the skin into the body, and cosmetics suitable for the skin type. You can select and consume inner beauty food that suits each individual by considering skin condition and lifestyle. More preferably, when the cosmetics containing the extract of Maesaengi and the inner beauty food containing the extract are mixed, the cell viability reduced by UV irradiation and proteins related to skin moisturizing and skin barrier (pillar) compared to using only cosmetics Green and involucrin) have the advantage of being able to see the effect of strengthening the skin barrier more effectively because the effect of increasing the expression level is significantly increased.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

Materials and Methods

1. Preparation of Maesaengi Ethanol Extract

After washing and drying the fresh celery (purchased from Wando) twice with distilled water and removing the water, 900 ml of 50% (v/v) ethanol was added to 150 g of the washed celery, and incubated at room temperature or 40 ° C for 6, 12 or 24 hours. After extraction, it was concentrated in a vacuum concentrator and lyophilized. As a result of comparing the yield according to various extraction conditions, it was confirmed that the highest yield was obtained when the maesaengi and ethanol mixture was extracted at 40 ° C. for 6 hours (Table 1).

2. Cell culture

The keratinocytes (HaCaT) used in the experiment were prepared in DMEM medium (Dulbecco's Modified Eagle Medium) containing 10% fetal bovine serum (FBS) and 1% antibiotics (penicillin/streptomycin) at 37°C and 5% CO ₂ cultured under the condition. The medium was exchanged every 2 days and subcultured by treatment with trypsin (trypsin-EDTA).

3. MTT assay

HaCaT cells were dispensed at 1×10 ³ cells/well in a 96-well culture vessel and cultured for 24 hours, and then treated with the extract of Maesaengi by concentration (25, 50 or 100 μg/ml) and cultured for 24 hours. The cultured cells were treated with MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tertrazolium bromide, 2mg/ml)] solution and allowed to react for 4 hours, then the MTT solution was removed and treated with DMSO. After reacting for 30 minutes in a dark room, absorbance was measured at 540 nm using an absorbance meter. Cell viability was calculated as a percentage (%) compared to the value of the control group in which the sample was not treated.

In addition, when measuring the cell viability according to ultraviolet (UVB) treatment, the HaCaT cell cultured 96-well culture container was pretreated for 1 hour by concentration, and UVB (300mJ) was added in PBS (phosphate buffered saline). / cm ² ) After irradiation, a serum-free medium was added and incubated for 24 hours, and then treated with MTT solution and reacted for 4 hours. After the reaction was completed, absorbance was measured in the same manner as described above.

4. Western blot

In order to perform Western blotting on HaCaT cells treated with ultraviolet (UVB), HaCaT cells were dispensed at 5×10 ⁴ cells/well in a 6-well culture vessel and cultured for 24 hours, and then the extracts of Maesaengi were prepared by concentration ( 25, 50 or 100 μg/ml) and pre-treated for 1 hour, irradiated with UVB (300mJ/cm ² ) in PBS, and then cultured for 24 hours in serum-free medium and then with PBS. After washing twice, protein was extracted by adding lysis buffer (RIPA buffer, 5% protease inhibitor cocktail), and centrifugation was performed at 13,000 rpm and 4° C. for 20 minutes to recover the layer solution. In the collected supernatant, proteins were quantified using Breadford reagent, and the quantified proteins were loaded on a 10% SDS-polyacrylamide gel, electrophoresed, and then nitrocellulose membrane ) was transferred to. After transfer, blocking with 5% skim milk, primary antibodies (NovusBio, USA) and secondary antibodies (Jackson ImmunoResearch, USA) of filaggrin and involucrin After reacting using the ECL kit, the protein expression level was confirmed.

Example 1. Analysis of cell viability of the extract of Maesaengi in HaCaT cells

To confirm the cytotoxicity of Maesaengi extract, HaCaT cells were treated with Maesaengi extract at different concentrations (25, 50, or 100 μg/ml) and MTT assay was performed. It was confirmed that the cell viability of was at a similar level (FIG. 1A). Through this, it was found that the ethanol extract of Maesaengi of the present invention was not toxic to skin cells.

In addition, in order to confirm the cytoprotective effect of Maesaengi extract, HaCaT cells were pretreated with Maesaengi extract by concentration, irradiated with UVB, and then MTT assay was performed. It was recovered, and in particular, it was confirmed that the cell viability increased up to 83.2% in the group treated with 100 μg/ml of Maesaengii extract (FIG. 1B). Through this, it was found that the ethanol extract of Maesaengi of the present invention has an excellent skin cell protection effect against skin damage caused by ultraviolet rays.

Example 2. Analysis of the expression level of skin moisturizing and skin barrier related proteins in HaCaT cells

As a result of analyzing the expression levels of filaggrin and involucrin through Western blot to examine the effect of Maesaengi extract on skin moisturizing and skin barrier function, the UVB alone treatment group compared to the control group (UVB and Maesaengi extract untreated) Although the expression levels of filaggrin and involucrin were significantly reduced by about 60%, it was confirmed that the expression level of filaggrin and involucrin was higher in the group irradiated with UVB after pretreatment with the extract of Maesaengi, compared to the group treated with UVB alone. In particular, in the group treated with 100 μg / ml of Maesaengi extract, it was found that the expression level of filaggrin and involucrin was about 92-98%, which was not significantly different from the normal level (control group) (FIG. 2).

Filaggrin is a type of epidermal protein, and is processed into forms such as profilaggrin, filaggrin, and natural moisturizing factor (NMF) in the process of generating the stratum corneum, and finally forms NMF. It constitutes 20 to 30% of the stratum corneum (based on dry weight) and has excellent water absorption properties, so it acts as a humectant to control the moisture in the stratum corneum. In addition, involucrin is a major structural component of the epidermis of keratinized cells. In normal epidermis, it begins to appear in the upper spinous layer and the granular layer, and after being synthesized, it is located close to the cell membrane and is converted to a membrane protein by transglutamidase. attached to serve as a support.

Therefore, the ethanol extract of Maesaengi of the present invention suppresses the loss of filaggrin and involucrin by ultraviolet irradiation and is excellent in moisturizing the skin and strengthening the skin barrier function, so cosmetics for improving skin damaged by external stimuli and It was thought that it could be used as a material for health functional food.

Claims (6)

A cosmetic composition for enhancing moisturizing of skin damaged by UV irradiation, consisting of an ethanol extract of Capsosiphon fulvescens as an active ingredient. delete delete delete A health functional food composition for enhancing moisturizing of skin damaged by UV irradiation, consisting of an ethanol extract of Capsosiphon fulvescens as an active ingredient. The functional food composition for moisturizing the skin according to claim 5, wherein the composition is an inner beauty food.